Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0005602

Physical Location & Seq

Genomic Coordinates Forward (+)

708775 .. 714180

Gene Structure 5406 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom12g00820.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1932 bp

ATGGGTTTATTGGTTGATTCAAAAGGGGAAGGGGGCGGCGGAGGAAGTGGAAGAAACGGCGGTTCATGGGGCGGCCGGCATAGTTTGATAAGGCGGAAACAGGTGGATTCTGTTCATGTGAAGTCAGGGGGTCATCAATTGGCCAAGGAGTTGACTGTTCCCCATCTTGTTGCAATTGGGGTTGGCTCAACGATTGGAGCAGGAGTCTATATTCTTGTTGGAACAGTTGCTAGAGAGCATTCTGGACCAGCACTAGCAATTTCCTTTCTAATAGCTGGAATTGCGGCAGCCCTTTCTGCATTTTGTTATGCTGAACTTGCAAGTCGGTGCCCTTCTGCAGGGAGTGCCTATCACTATACATATATATGCATTGGAGAAGGTGTCGCTTGGTTAGTTGGTTGGGCTTTGATTCTAGAATATACAATTGGTGGCTCAGCTGTTGCTCGGGGCATATCACCTAATTTGGCATTGCTTTTTGGAGGAGAGGACAGTCTGCCTGCTATTATAGCCCGGCAGCATATTCCTGCACTTGGTCTTGTGGTTGACCCATGTGCAGCAATTCTAGTATTTATTGTTACAGGGCTCTTGTGTGTGGGAATCAAGGAGAGTACAGTAGCACAAGGCATTGTGACGATAGCGAATGTATGTGCAATGATATTTGTTGTAGTAGCTGGTACATACTTGGGTTTCAAGTCTGGCTGGACTGGATATGAACTTCCTACTGGGTACTTTCCCTTTGGGGTGAATGGTATGCTTGCTGGGTCTGCAACTGTCTTCTTTGCATACATTGGTTTTGATTCAGTTGCCAGCACTGCTGAAGAGGTGAAGAATCCTCAACGAGATTTGCCACTTGGTATTGGTGCTGCACTATCTATTTGCTGTGCGTTGTACATGTTGGTTTCGATGGTCATTGTTGGTTTAGTGCCTTATTATGCAATGGATCCTGATACACCAATTTCCAGTGCATTTGCTAGTCATGGGATGCAATGGGCAGCGTATGTGATAACTGTTGGAGCTGTTACTGCTCTCTGCTCAACATTGATGGGCTCTATTCTTCCTCAGCCACGAATCCTGATGGCCATGTCTAGAGATGGATTGCTGCCACCATTTTTTGCAGATATTAGTACAAAAACTCAGGTTCCTGTCAAGAGTACAATAGCAACTGGGATTTGTTGTGCAGTCTTGGCCTTCTTCATGGATGTTTCACAGTTGGCAGGAATGGTTAGTGTGGGTACACTTGTTGCATTCACTATGGTAGCAATATCTGTACTGATACTCAGATATGTTCCGCCAGATGAGGTGCCTATGCCTTCATCTCTTCAGGAGTCAATAGATTCAGTGTGGTTACGACATGGAAGGAATTCTCAAGAGATCAATGTTGACGATCCTAAGACTAGTGTTACCAAACCTCTACTCGAGGAAGTGAATATCACGGTTGAACTTCCTCTGATAAAAAAGCAACTGCCTCTTATTAACTACACACTAAGTGAAGCTAAAAGGCGTAAAATTGCTGGCTGGACCATAATGTTCACATGTGTCGGCGTGCTTCTCCTGACATATTCAGCTTCAGATTTGGCCATTCCCAGCTTTCTTCGCTATACATTTTGTGGAGGTGGCGGTATTCTTCTTTTGTTAGGTCTGATTGTACTCACATGTATTGATCAAGATGATGCACGACACACCTTTGGGCACACAGGAGGTTTCTTATGCCCATTTGTCCCATTGCTACCTATCATCTGTATTCTCATCAACGTCTACCTACTGATAAATCTTGGAGCGGACACCTGGGCGCGTGTTGCTGTATGGCTGCTAATAGGAGTGGTGGTTTACGTATTTTATGGCCGATCACACAGCTCATTGGAGGATGCAGTTTACGTGCCAGTAGCTCACGCTGATGAGATCTTTCGGAGCTCAGAAACTTATGTAGCGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000325 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005737 GO:0005773 GO:0005774 GO:0005783 GO:0008150 GO:0009705 GO:0012505 GO:0016020 GO:0031090 GO:0031967 GO:0031975 GO:0042592 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044428 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0048878 GO:0050801 GO:0055081 GO:0065007 GO:0065008 GO:0080144 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko05206 map05206

Pfam Domains

Protein Families

AA_permease_2 AA_permease_C

Protein Analysis

644

Amino Acids

67.97

Weight (kDa)

6.01

Isoelectric Point (pI)

35.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
AA_permease_2	PF13520	53 - 433	8.9e-53	Amino acid permease
AA_permease	PF00324	55 - 425	7.3e-40	Amino acid permease
AA_permease_C	PF13906	568 - 618	6.6e-22	C-terminus of AA_permease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0005602)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G58030 AT5G36940
fragaria_vesca	FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560 FvH4_1g28560
malus_domestica	MD12G1010500.v1.1 MD14G1007600.v1.1
prunus_persica	Prupe.7G012500_v2.0.a1 Prupe.7G012500_v2.0.a1 Prupe.7G012500_v2.0.a1
pyrus_communis	pycom12g00820 pycom14g00610
rosa_chinensis	RchiOBHm_Chr3g0496211
rosa_laevigata	RLG00000022551
rosa_roxburghii	Rroxscaffold_6G00389450
rosa_rugosa	Rorug03G0280500
rosa_samantha	Rh3AG327600 Rh3BG363200 Rh3BG363600 Rh3CG360600 Rh3DG363600
rosa_wichuraiana	Rw3G028690

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	327, 1300
AccBSI	CCGCTC	1 cut(s)	1778
AccI	GTMKAC	1 cut(s)	1755
AccII	CGCG	1 cut(s)	1792
AciI	CCGC	9 cut(s)	36, 39, 60, 72, 94, 284, 1289, 1617, 1778
AclWI	GGATC	3 cut(s)	935, 948, 1379
AcoI	YGGCCR	5 cut(s)	73, 141, 1077, 1575, 1840
AcsI	RAATTY	1 cut(s)	1360
AcuI	CTGAAG	3 cut(s)	837, 1304, 1551
AdeI	CACNNNGTG	1 cut(s)	1487
AfaI	GTAC	9 cut(s)	610, 676, 728, 890, 1126, 1153, 1234, 1269, 1647
AfiI	CCNNNNNNNGG	2 cut(s)	338, 530
AflIII	ACRYGT	3 cut(s)	891, 1532, 1652
AgsI	TTSAA	3 cut(s)	21, 691, 1439
AhlI	ACTAGT	1 cut(s)	1394
AjnI	CCWGG	1 cut(s)	1784
AjuI	GAANNNNNNNTTGG	2 cut(s)	408, 440
Alw21I	GWGCWC	1 cut(s)	1913
AlwI	GGATC	3 cut(s)	935, 948, 1379
Ama87I	CYCGRG	2 cut(s)	444, 1415
AoxI	GGCC	6 cut(s)	73, 141, 1077, 1185, 1575, 1840
ApeKI	GCWGC	7 cut(s)	287, 514, 554, 863, 992, 1099, 1807
ApoI	RAATTY	1 cut(s)	1360
Asp700I	GAANNNNTTC	1 cut(s)	1360
AspLEI	GCGC	1 cut(s)	1792
AspS9I	GGNCC	2 cut(s)	245, 1518
AsuC2I	CCSGG	1 cut(s)	511
AsuHPI	GGTGA	3 cut(s)	447, 754, 835
AvaI	CYCGRG	2 cut(s)	444, 1415
AvaII	GGWCC	2 cut(s)	245, 1518
BaeGI	GKGCMC	2 cut(s)	332, 1692
BaeI	ACNNNNGTAYC	2 cut(s)	1224, 1257
BalI	TGGCCA	3 cut(s)	143, 1079, 1577
BamHI	GGATCC	1 cut(s)	940
BanI	GGYRCC	2 cut(s)	327, 1300
BanII	GRGCYC	3 cut(s)	585, 1049, 1913
BbsI	GAAGAC	1 cut(s)	766
Bbv12I	GWGCWC	1 cut(s)	1913
BbvCI	CCTCAGC	1 cut(s)	1059
BbvI	GCAGC	7 cut(s)	299, 526, 566, 850, 1004, 1086, 1794
BccI	CCATC	5 cut(s)	171, 898, 1036, 1069, 1085
BceAI	ACGGC	1 cut(s)	73
BciT130I	CCWGG	1 cut(s)	1786
BclI	TGATCA	1 cut(s)	1660
BcnI	CCSGG	1 cut(s)	511
BcuI	ACTAGT	1 cut(s)	1394
BfaI	CTAG	7 cut(s)	231, 254, 413, 563, 972, 1086, 1395
BfmI	CTRYAG	1 cut(s)	336
BglII	AGATCT	1 cut(s)	1899
BlpI	GCTNAGC	1 cut(s)	433
Bme1390I	CCNGG	2 cut(s)	511, 1786
Bme18I	GGWCC	2 cut(s)	245, 1518
BmeT110I	CYCGRG	2 cut(s)	444, 1415
BmgT120I	GGNCC	2 cut(s)	245, 1518
BmiI	GGNNCC	4 cut(s)	329, 942, 1140, 1302
BmrFI	CCNGG	2 cut(s)	511, 1786
BmrI	ACTGGG	2 cut(s)	732, 1173
BmsI	GCATC	3 cut(s)	972, 1660, 1855
BmuI	ACTGGG	2 cut(s)	732, 1173
BpiI	GAAGAC	1 cut(s)	766
Bpu10I	CCTNAGC	1 cut(s)	1059
Bpu1102I	GCTNAGC	1 cut(s)	433
BpuEI	CTTGAG	1 cut(s)	1350
BpuMI	CCSGG	1 cut(s)	511
BsaAI	YACGTR	2 cut(s)	1831, 1876
BsaJI	CCNNGG	2 cut(s)	144, 1785
BsaXI	ACNNNNNCTCC	6 cut(s)	189, 219, 366, 396, 1809, 1839
Bsc4I	CCNNNNNNNGG	2 cut(s)	338, 530
Bse118I	RCCGGY	1 cut(s)	75
Bse1I	ACTGG	5 cut(s)	709, 727, 960, 1168, 1880
Bse3DI	GCAATG	5 cut(s)	467, 657, 942, 992, 1721
BseBI	CCWGG	1 cut(s)	1786
BseDI	CCNNGG	2 cut(s)	144, 1785
BseGI	GGATG	3 cut(s)	987, 1204, 1870
BseLI	CCNNNNNNNGG	2 cut(s)	338, 530
BseMI	GCAATG	5 cut(s)	467, 657, 942, 992, 1721
BseMII	CTCAG	5 cut(s)	447, 1073, 1148, 1291, 1926
BseNI	ACTGG	5 cut(s)	709, 727, 960, 1168, 1880
BseRI	GAGGAG	1 cut(s)	495
BseSI	GKGCMC	2 cut(s)	332, 1692
BseX3I	CGGCCG	1 cut(s)	73
BseXI	GCAGC	7 cut(s)	299, 526, 566, 850, 1004, 1086, 1794
BseYI	CCCAGC	2 cut(s)	758, 1583
BsgI	GTGCAG	4 cut(s)	510, 573, 849, 1197
Bsh1236I	CGCG	1 cut(s)	1792
Bsh1285I	CGRYCG	1 cut(s)	76
BshFI	GGCC	6 cut(s)	75, 143, 1079, 1187, 1577, 1842
BshNI	GGYRCC	2 cut(s)	327, 1300
BsiEI	CGRYCG	1 cut(s)	76
BsiHKAI	GWGCWC	1 cut(s)	1913
BsiHKCI	CYCGRG	2 cut(s)	444, 1415
BsiSI	CCGG	2 cut(s)	76, 511
BslFI	GGGAC	1 cut(s)	1703
BslI	CCNNNNNNNGG	2 cut(s)	338, 530
BsmFI	GGGAC	1 cut(s)	1703
BsmI	GAATGC	2 cut(s)	238, 1244
BsnI	GGCC	6 cut(s)	75, 143, 1079, 1187, 1577, 1842
BsoBI	CYCGRG	2 cut(s)	444, 1415
Bsp1286I	GDGCHC	5 cut(s)	332, 585, 1049, 1692, 1913
Bsp1407I	TGTACA	1 cut(s)	888
Bsp143I	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
Bsp1720I	GCTNAGC	1 cut(s)	433
BspACI	CCGC	9 cut(s)	36, 39, 60, 72, 94, 284, 1289, 1617, 1778
BspANI	GGCC	6 cut(s)	75, 143, 1079, 1187, 1577, 1842
BspCNI	CTCAG	5 cut(s)	446, 1072, 1147, 1290, 1925
BspFNI	CGCG	1 cut(s)	1792
BspLI	GGNNCC	4 cut(s)	329, 942, 1140, 1302
BspMAI	CTGCAG	1 cut(s)	340
BspPI	GGATC	3 cut(s)	935, 948, 1379
BspT107I	GGYRCC	2 cut(s)	327, 1300
BsrBI	CCGCTC	1 cut(s)	1778
BsrDI	GCAATG	5 cut(s)	467, 657, 942, 992, 1721
BsrFI	RCCGGY	1 cut(s)	75
BsrGI	TGTACA	1 cut(s)	888
BsrI	ACTGG	5 cut(s)	709, 727, 960, 1168, 1880
BssAI	RCCGGY	1 cut(s)	75
BssECI	CCNNGG	2 cut(s)	144, 1785
BssMI	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
BssT1I	CCWWGG	1 cut(s)	144
Bst2UI	CCWGG	1 cut(s)	1786
Bst4CI	ACNGT	8 cut(s)	157, 226, 491, 613, 772, 1009, 1209, 1435
Bst6I	CTCTTC	2 cut(s)	813, 1323
BstAPI	GCANNNNNTGC	1 cut(s)	344
BstAUI	TGTACA	1 cut(s)	888
BstBAI	YACGTR	2 cut(s)	1831, 1876
BstC8I	GCNNGC	6 cut(s)	77, 498, 756, 808, 1513, 1544
BstDEI	CTNAG	7 cut(s)	433, 1059, 1134, 1277, 1389, 1484, 1912
BstENI	CCTNNNNNAGG	1 cut(s)	336
BstF5I	GGATG	3 cut(s)	987, 1204, 1870
BstFNI	CGCG	1 cut(s)	1792
BstHHI	GCGC	1 cut(s)	1792
BstKTI	GATC	6 cut(s)	943, 1374, 1387, 1663, 1847, 1902
BstMBI	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
BstMCI	CGRYCG	1 cut(s)	76
BstNI	CCWGG	1 cut(s)	1786
BstNSI	RCATGY	3 cut(s)	895, 1536, 1656
BstSCI	CCNGG	2 cut(s)	509, 1784
BstSFI	CTRYAG	1 cut(s)	336
BstSLI	GKGCMC	2 cut(s)	332, 1692
BstSNI	TACGTA	1 cut(s)	1831
BstUI	CGCG	1 cut(s)	1792
BstV1I	GCAGC	7 cut(s)	299, 526, 566, 850, 1004, 1086, 1794
BstV2I	GAAGAC	1 cut(s)	766
BstX2I	RGATCY	2 cut(s)	940, 1899
BstYI	RGATCY	2 cut(s)	940, 1899
BstZI	CGGCCG	1 cut(s)	73
BsuRI	GGCC	6 cut(s)	75, 143, 1079, 1187, 1577, 1842
BtsCI	GGATG	3 cut(s)	987, 1204, 1870
BtsI	GCAGTG	1 cut(s)	810
BtsIMutI	CAGTG	3 cut(s)	810, 967, 1344
Cac8I	GCNNGC	6 cut(s)	77, 498, 756, 808, 1513, 1544
CfoI	GCGC	1 cut(s)	1792
Cfr10I	RCCGGY	1 cut(s)	75
Cfr13I	GGNCC	2 cut(s)	245, 1518
Csp6I	GTAC	9 cut(s)	609, 675, 727, 889, 1125, 1152, 1233, 1268, 1646
CviQI	GTAC	9 cut(s)	609, 675, 727, 889, 1125, 1152, 1233, 1268, 1646
DdeI	CTNAG	7 cut(s)	433, 1059, 1134, 1277, 1389, 1484, 1912
DpnI	GATC	6 cut(s)	942, 1373, 1386, 1662, 1846, 1901
DpnII	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
DraIII	CACNNNGTG	1 cut(s)	1487
EaeI	YGGCCR	5 cut(s)	73, 141, 1077, 1575, 1840
EagI	CGGCCG	1 cut(s)	73
Eam1104I	CTCTTC	2 cut(s)	813, 1323
EarI	CTCTTC	2 cut(s)	813, 1323
EciI	GGCGGA	3 cut(s)	54, 109, 1278
Ecl136II	GAGCTC	1 cut(s)	1911
EclXI	CGGCCG	1 cut(s)	73
Eco105I	TACGTA	1 cut(s)	1831
Eco130I	CCWWGG	1 cut(s)	144
Eco24I	GRGCYC	3 cut(s)	585, 1049, 1913
Eco47I	GGWCC	2 cut(s)	245, 1518
Eco52I	CGGCCG	1 cut(s)	73
Eco53kI	GAGCTC	1 cut(s)	1911
Eco57I	CTGAAG	3 cut(s)	837, 1304, 1551
Eco88I	CYCGRG	2 cut(s)	444, 1415
EcoICRI	GAGCTC	1 cut(s)	1911
EcoNI	CCTNNNNNAGG	1 cut(s)	336
EcoRI	GAATTC	1 cut(s)	1360
EcoRII	CCWGG	1 cut(s)	1784
EcoT14I	CCWWGG	1 cut(s)	144
EcoT22I	ATGCAT	1 cut(s)	371
EcoT38I	GRGCYC	3 cut(s)	585, 1049, 1913
ErhI	CCWWGG	1 cut(s)	144
FaqI	GGGAC	1 cut(s)	1703
FbaI	TGATCA	1 cut(s)	1660
FblI	GTMKAC	1 cut(s)	1755
FokI	GGATG	3 cut(s)	994, 1211, 1877
FriOI	GRGCYC	3 cut(s)	585, 1049, 1913
FspBI	CTAG	7 cut(s)	231, 254, 413, 563, 972, 1086, 1395
GlaI	GCGC	1 cut(s)	1791
GsaI	CCCAGC	2 cut(s)	762, 1587
HaeIII	GGCC	6 cut(s)	75, 143, 1079, 1187, 1577, 1842
HapII	CCGG	2 cut(s)	76, 511
HhaI	GCGC	1 cut(s)	1792
Hin6I	GCGC	1 cut(s)	1790
HinP1I	GCGC	1 cut(s)	1790
HincII	GTYRAC	3 cut(s)	153, 544, 1381
HindII	GTYRAC	3 cut(s)	153, 544, 1381
HpaII	CCGG	2 cut(s)	76, 511
HphI	GGTGA	3 cut(s)	447, 754, 835
Hpy166II	GTNNAC	8 cut(s)	153, 544, 1235, 1381, 1530, 1756, 1828, 1873
Hpy188I	TCNGA	6 cut(s)	1280, 1449, 1570, 1641, 1908, 1915
Hpy8I	GTNNAC	8 cut(s)	153, 544, 1235, 1381, 1530, 1756, 1828, 1873
HpyAV	CCTTC	6 cut(s)	23, 342, 371, 1198, 1320, 1350
HpyCH4III	ACNGT	8 cut(s)	157, 226, 491, 613, 772, 1009, 1209, 1435
HpyCH4IV	ACGT	3 cut(s)	1752, 1830, 1875
HpyF3I	CTNAG	7 cut(s)	433, 1059, 1134, 1277, 1389, 1484, 1912
HpySE526I	ACGT	3 cut(s)	1752, 1830, 1875
HspAI	GCGC	1 cut(s)	1790
KroI	GCCGGC	1 cut(s)	75
KroNI	GCCGGC	1 cut(s)	77
Ksp22I	TGATCA	1 cut(s)	1660
Kzo9I	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
LmnI	GCTCC	4 cut(s)	197, 1013, 1775, 1908
Lsp1109I	GCAGC	7 cut(s)	299, 526, 566, 850, 1004, 1086, 1794
LweI	GCATC	3 cut(s)	972, 1660, 1855
MaeI	CTAG	7 cut(s)	231, 254, 413, 563, 972, 1086, 1395
MaeII	ACGT	3 cut(s)	1752, 1830, 1875
MaeIII	GTNAC	5 cut(s)	574, 628, 1018, 1344, 1399
MalI	GATC	6 cut(s)	942, 1373, 1386, 1662, 1846, 1901
MbiI	CCGCTC	1 cut(s)	1778
MboI	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
MboII	GAAGA	9 cut(s)	63, 766, 830, 838, 1046, 1183, 1310, 1583, 1616
MfeI	CAATTG	3 cut(s)	137, 174, 423
MflI	RGATCY	2 cut(s)	940, 1899
MhlI	GDGCHC	5 cut(s)	332, 585, 1049, 1692, 1913
MlsI	TGGCCA	3 cut(s)	143, 1079, 1577
MluNI	TGGCCA	3 cut(s)	143, 1079, 1577
MlyI	GAGTC	2 cut(s)	213, 1334
MmeI	TCCRAC	2 cut(s)	199, 991
Mox20I	TGGCCA	3 cut(s)	143, 1079, 1577
Mph1103I	ATGCAT	1 cut(s)	371
MroNI	GCCGGC	1 cut(s)	75
MroXI	GAANNNNTTC	1 cut(s)	1360
MscI	TGGCCA	3 cut(s)	143, 1079, 1577
MseI	TTAA	1 cut(s)	1473
MslI	CAYNNNNRTG	2 cut(s)	364, 1893
Msp20I	TGGCCA	3 cut(s)	143, 1079, 1577
MspA1I	CMGCKG	1 cut(s)	437
MspI	CCGG	2 cut(s)	76, 511
MspR9I	CCNGG	2 cut(s)	511, 1786
MunI	CAATTG	3 cut(s)	137, 174, 423
Mva1269I	GAATGC	2 cut(s)	238, 1244
MvaI	CCWGG	1 cut(s)	1786
MvnI	CGCG	1 cut(s)	1792
NaeI	GCCGGC	1 cut(s)	77
NciI	CCSGG	1 cut(s)	511
NdeII	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
NgoMIV	GCCGGC	1 cut(s)	75
NlaIV	GGNNCC	4 cut(s)	329, 942, 1140, 1302
NmuCI	GTSAC	1 cut(s)	628
NsiI	ATGCAT	1 cut(s)	371
NspI	RCATGY	3 cut(s)	895, 1536, 1656
PaeR7I	CTCGAG	1 cut(s)	1415
PciI	ACATGT	3 cut(s)	891, 1532, 1652
PctI	GAATGC	2 cut(s)	238, 1244
PdiI	GCCGGC	1 cut(s)	77
PdmI	GAANNNNTTC	1 cut(s)	1360
PfeI	GAWTC	8 cut(s)	17, 107, 409, 597, 797, 829, 1068, 1334
PleI	GAGTC	2 cut(s)	212, 1333
PpsI	GAGTC	2 cut(s)	212, 1333
Ppu21I	YACGTR	2 cut(s)	1831, 1876
PscI	ACATGT	3 cut(s)	891, 1532, 1652
Psp124BI	GAGCTC	1 cut(s)	1913
Psp6I	CCWGG	1 cut(s)	1784
PspFI	CCCAGC	2 cut(s)	758, 1583
PspGI	CCWGG	1 cut(s)	1784
PspN4I	GGNNCC	4 cut(s)	329, 942, 1140, 1302
PspPI	GGNCC	2 cut(s)	245, 1518
PspXI	VCTCGAGB	1 cut(s)	1415
PstI	CTGCAG	1 cut(s)	340
PsuI	RGATCY	2 cut(s)	940, 1899
PvuII	CAGCTG	1 cut(s)	437
RsaI	GTAC	9 cut(s)	610, 676, 728, 890, 1126, 1153, 1234, 1269, 1647
RsaNI	GTAC	9 cut(s)	609, 675, 727, 889, 1125, 1152, 1233, 1268, 1646
RseI	CAYNNNNRTG	2 cut(s)	364, 1893
SacI	GAGCTC	1 cut(s)	1913
SaqAI	TTAA	1 cut(s)	1473
Sau3AI	GATC	6 cut(s)	940, 1371, 1384, 1660, 1844, 1899
Sau96I	GGNCC	2 cut(s)	245, 1518
SchI	GAGTC	2 cut(s)	213, 1334
ScrFI	CCNGG	2 cut(s)	511, 1786
SduI	GDGCHC	5 cut(s)	332, 585, 1049, 1692, 1913
SfaNI	GCATC	3 cut(s)	972, 1660, 1855
SfcI	CTRYAG	1 cut(s)	336
Sfr274I	CTCGAG	1 cut(s)	1415
SinI	GGWCC	2 cut(s)	245, 1518
SlaI	CTCGAG	1 cut(s)	1415
SmiMI	CAYNNNNRTG	2 cut(s)	364, 1893
SmlI	CTYRAG	2 cut(s)	1365, 1415
SmoI	CTYRAG	2 cut(s)	1365, 1415
SnaBI	TACGTA	1 cut(s)	1831
SpeI	ACTAGT	1 cut(s)	1394
SsiI	CCGC	9 cut(s)	36, 39, 60, 72, 94, 284, 1289, 1617, 1778
SspMI	CTAG	7 cut(s)	231, 254, 413, 563, 972, 1086, 1395
SstI	GAGCTC	1 cut(s)	1913
StyD4I	CCNGG	2 cut(s)	509, 1784
StyI	CCWWGG	1 cut(s)	144
TaaI	ACNGT	8 cut(s)	157, 226, 491, 613, 772, 1009, 1209, 1435
TaiI	ACGT	3 cut(s)	1755, 1833, 1878
TaqI	TCGA	2 cut(s)	902, 1416
TatI	WGTACW	6 cut(s)	608, 888, 1124, 1151, 1267, 1645
TauI	GCSGC	3 cut(s)	39, 75, 287
TfiI	GAWTC	8 cut(s)	17, 107, 409, 597, 797, 829, 1068, 1334
Tru1I	TTAA	1 cut(s)	1473
Tru9I	TTAA	1 cut(s)	1473
TscAI	CASTG	3 cut(s)	817, 967, 1344
TseFI	GTSAC	1 cut(s)	628
TseI	GCWGC	7 cut(s)	287, 514, 554, 863, 992, 1099, 1807
Tsp45I	GTSAC	1 cut(s)	628
TspDTI	ATGAA	5 cut(s)	54, 104, 726, 1183, 1302
TspRI	CASTG	3 cut(s)	817, 967, 1344
VpaK11BI	GGWCC	2 cut(s)	245, 1518
XagI	CCTNNNNNAGG	1 cut(s)	336
XapI	RAATTY	1 cut(s)	1360
XbaI	TCTAGA	2 cut(s)	412, 1085
XceI	RCATGY	3 cut(s)	895, 1536, 1656
XhoI	CTCGAG	1 cut(s)	1415
XmiI	GTMKAC	1 cut(s)	1755
XmnI	GAANNNNTTC	1 cut(s)	1360
XspI	CTAG	7 cut(s)	231, 254, 413, 563, 972, 1086, 1395
Zsp2I	ATGCAT	1 cut(s)	371

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.