pycom12g01230

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010748
Physical Location & Seq
Reverse (-)
1057824 .. 1059267
1444 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 714 bp
ATGTTAGAAGTTGTTACCATTATTCTGCTTGATTGTGAAATTCTTTCATCGTGTCAGAACACGAATGCAGTAAACAAGCAGATCCTGTATTTGGTATACTGCATGGAGAATGCCATTCTGAATATGAGTTCAGATCAGGAACAGATGGTGTGGCTAATCGATTTTCAACGGTGGAACACATCGAGCATGTCGTTGAAGGCAACTAGGGAAACAGCAAAGATCCTGCAAAATCATTACCCTGAGAGATTGGGAATTGGCATCCTCTATAATCCACCAAAAGTATTTGAGTCCTTTTGGACGATGGTAAAACCATTTCTTGAGCCGAAAACACACAAGAAGGTGAAGTTTGTGTACTCGGACAACCCACAGAGCAAGAAGGTAATGGAAGATCTTTTCAACATGGACAAGTTGGAATCTGCATTTGGTGGAAGAAGTTCAGGTGGTTTTGACTATGAAGCTTACGGACAAAGAATGCGAGAAGATGACAAGAAAATGTCAAGTTTCGTTGACTCTGGCTGTTCTTCAGCGTACTCGCCATCAATCATGTCTGAGTTGCAGCGGTCGGAGTTGTCTTCTGACCAATGTTCCGAGGTCTCTGATGAAGGCGGTTGCTCGTCGTGCGACAAAGCAGCTTCGTCGAACTTGGAGGTTGCCGATGAGAAGGTACAAGGACAACCGCACGACATCAAAGATGTCGCAAATGGCAAGACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

238

Amino Acids

26.69

Weight (kDa)

4.94

Isoelectric Point (pI)

59.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 20 - 143 2e-27 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 96
AciI CCGC 3 cut(s) 559, 606, 677
AclWI GGATC 2 cut(s) 76, 214
AcsI RAATTY 1 cut(s) 39
AcuI CTGAAG 1 cut(s) 507
AfaI GTAC 3 cut(s) 353, 530, 666
AfiI CCNNNNNNNGG 1 cut(s) 91
AgsI TTSAA 3 cut(s) 167, 196, 397
AjuI GAANNNNNNNTTGG 2 cut(s) 405, 437
AluBI AGCT 2 cut(s) 458, 632
AluI AGCT 2 cut(s) 458, 632
Alw26I GTCTC 1 cut(s) 598
AlwI GGATC 2 cut(s) 76, 214
AlwNI CAGNNNCTG 1 cut(s) 85
ApeKI GCWGC 2 cut(s) 556, 629
ApoI RAATTY 1 cut(s) 39
Asp700I GAANNNNTTC 1 cut(s) 433
AsuHPI GGTGA 1 cut(s) 352
BarI GAAGNNNNNNTAC 2 cut(s) 335, 367
BbsI GAAGAC 1 cut(s) 564
BbvI GCAGC 2 cut(s) 568, 641
BccI CCATC 3 cut(s) 139, 295, 544
BcgI CGANNNNNNTGC 2 cut(s) 618, 652
BcoDI GTCTC 1 cut(s) 598
BfaI CTAG 1 cut(s) 204
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 557, 630
BlsI GCNGC 2 cut(s) 558, 631
BmsI GCATC 1 cut(s) 267
BpiI GAAGAC 1 cut(s) 564
BpuEI CTTGAG 1 cut(s) 338
Bsa29I ATCGAT 1 cut(s) 159
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 1 cut(s) 588
Bsc4I CCNNNNNNNGG 1 cut(s) 91
BseCI ATCGAT 1 cut(s) 159
BseDI CCNNGG 1 cut(s) 588
BseGI GGATG 1 cut(s) 258
BseLI CCNNNNNNNGG 1 cut(s) 91
BseMII CTCAG 2 cut(s) 231, 540
BseXI GCAGC 2 cut(s) 568, 641
Bsh1285I CGRYCG 1 cut(s) 563
BshVI ATCGAT 1 cut(s) 159
BsiEI CGRYCG 1 cut(s) 563
BslI CCNNNNNNNGG 1 cut(s) 91
BsmAI GTCTC 1 cut(s) 598
BsmI GAATGC 3 cut(s) 70, 115, 477
Bso31I GGTCTC 1 cut(s) 598
Bsp143I GATC 4 cut(s) 81, 133, 219, 388
BspACI CCGC 3 cut(s) 559, 606, 677
BspCNI CTCAG 2 cut(s) 232, 541
BspDI ATCGAT 1 cut(s) 159
BspPI GGATC 2 cut(s) 76, 214
BspTNI GGTCTC 1 cut(s) 598
BssECI CCNNGG 1 cut(s) 588
BssMI GATC 4 cut(s) 81, 133, 219, 388
BssNAI GTATAC 1 cut(s) 97
Bst1107I GTATAC 1 cut(s) 97
Bst4CI ACNGT 1 cut(s) 171
BstDEI CTNAG 2 cut(s) 240, 549
BstF5I GGATG 1 cut(s) 258
BstKTI GATC 4 cut(s) 84, 136, 222, 391
BstMAI GTCTC 1 cut(s) 598
BstMBI GATC 4 cut(s) 81, 133, 219, 388
BstMCI CGRYCG 1 cut(s) 563
BstMWI GCNNNNNNNGC 1 cut(s) 618
BstNSI RCATGY 1 cut(s) 190
BstV1I GCAGC 2 cut(s) 568, 641
BstV2I GAAGAC 1 cut(s) 564
BstX2I RGATCY 3 cut(s) 81, 219, 388
BstYI RGATCY 3 cut(s) 81, 219, 388
BstZ17I GTATAC 1 cut(s) 97
Bsu15I ATCGAT 1 cut(s) 159
BsuTUI ATCGAT 1 cut(s) 159
BtsCI GGATG 1 cut(s) 258
CaiI CAGNNNCTG 1 cut(s) 85
ClaI ATCGAT 1 cut(s) 159
Csp6I GTAC 3 cut(s) 352, 529, 665
CviAII CATG 4 cut(s) 103, 187, 400, 544
CviJI RGCY 5 cut(s) 154, 322, 458, 516, 632
CviKI_1 RGCY 5 cut(s) 154, 322, 458, 516, 632
CviQI GTAC 3 cut(s) 352, 529, 665
DdeI CTNAG 2 cut(s) 240, 549
DpnI GATC 4 cut(s) 83, 135, 221, 390
DpnII GATC 4 cut(s) 81, 133, 219, 388
Eco31I GGTCTC 1 cut(s) 598
Eco57I CTGAAG 1 cut(s) 507
FaeI CATG 4 cut(s) 106, 190, 403, 547
FaiI YATR 9 cut(s) 97, 104, 125, 188, 267, 401, 453, 545, 712
FatI CATG 4 cut(s) 102, 186, 399, 543
FblI GTMKAC 1 cut(s) 96
Fnu4HI GCNGC 2 cut(s) 557, 630
FokI GGATG 1 cut(s) 245
Fsp4HI GCNGC 2 cut(s) 557, 630
FspBI CTAG 1 cut(s) 204
GluI GCNGC 2 cut(s) 557, 630
Hin1II CATG 4 cut(s) 106, 190, 403, 547
HincII GTYRAC 1 cut(s) 508
HindII GTYRAC 1 cut(s) 508
HindIII AAGCTT 1 cut(s) 456
HinfI GANTC 3 cut(s) 287, 413, 509
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 4 cut(s) 73, 97, 352, 508
Hpy188I TCNGA 9 cut(s) 57, 120, 133, 358, 550, 565, 577, 589, 598
Hpy188III TCNNGA 2 cut(s) 137, 317
Hpy8I GTNNAC 4 cut(s) 73, 97, 352, 508
Hpy99I CGWCG 2 cut(s) 619, 640
HpyAV CCTTC 5 cut(s) 190, 331, 370, 596, 655
HpyCH4III ACNGT 1 cut(s) 171
HpyCH4V TGCA 5 cut(s) 68, 102, 226, 419, 556
HpyF10VI GCNNNNNNNGC 1 cut(s) 618
HpyF3I CTNAG 2 cut(s) 240, 549
Hsp92II CATG 4 cut(s) 106, 190, 403, 547
Kzo9I GATC 4 cut(s) 81, 133, 219, 388
LpnPI CCDG 6 cut(s) 98, 122, 236, 252, 423, 498
Lsp1109I GCAGC 2 cut(s) 568, 641
LweI GCATC 1 cut(s) 267
MaeI CTAG 1 cut(s) 204
MaeIII GTNAC 1 cut(s) 13
MalI GATC 4 cut(s) 83, 135, 221, 390
MboI GATC 4 cut(s) 81, 133, 219, 388
MboII GAAGA 5 cut(s) 398, 441, 491, 513, 564
MflI RGATCY 3 cut(s) 81, 219, 388
MluCI AATT 2 cut(s) 39, 252
MlyI GAGTC 2 cut(s) 296, 503
MmeI TCCRAC 2 cut(s) 390, 543
MnlI CCTC 3 cut(s) 272, 583, 640
MroXI GAANNNNTTC 1 cut(s) 433
MspA1I CMGCKG 1 cut(s) 559
Mva1269I GAATGC 3 cut(s) 70, 115, 477
MwoI GCNNNNNNNGC 1 cut(s) 618
NdeII GATC 4 cut(s) 81, 133, 219, 388
NlaIII CATG 4 cut(s) 106, 190, 403, 547
NspI RCATGY 1 cut(s) 190
PcsI WCGNNNNNNNCGW 1 cut(s) 188
PctI GAATGC 3 cut(s) 70, 115, 477
PdmI GAANNNNTTC 1 cut(s) 433
PfeI GAWTC 1 cut(s) 413
PkrI GCNGC 2 cut(s) 558, 631
PleI GAGTC 2 cut(s) 295, 503
PpsI GAGTC 2 cut(s) 295, 503
PstNI CAGNNNCTG 1 cut(s) 85
PsuI RGATCY 3 cut(s) 81, 219, 388
RsaI GTAC 3 cut(s) 353, 530, 666
RsaNI GTAC 3 cut(s) 352, 529, 665
SatI GCNGC 2 cut(s) 557, 630
Sau3AI GATC 4 cut(s) 81, 133, 219, 388
SchI GAGTC 2 cut(s) 296, 503
SetI ASST 8 cut(s) 342, 381, 442, 460, 594, 634, 651, 666
SfaNI GCATC 1 cut(s) 267
SmlI CTYRAG 1 cut(s) 317
SmoI CTYRAG 1 cut(s) 317
Sse9I AATT 2 cut(s) 39, 252
SsiI CCGC 3 cut(s) 559, 606, 677
SspMI CTAG 1 cut(s) 204
TaaI ACNGT 1 cut(s) 171
TaqI TCGA 3 cut(s) 159, 182, 638
TasI AATT 2 cut(s) 39, 252
TatI WGTACW 1 cut(s) 351
TfiI GAWTC 1 cut(s) 413
TseI GCWGC 2 cut(s) 556, 629
TspDTI ATGAA 3 cut(s) 36, 468, 615
TspGWI ACGGA 1 cut(s) 477
XapI RAATTY 1 cut(s) 39
XceI RCATGY 1 cut(s) 190
XmiI GTMKAC 1 cut(s) 96
XmnI GAANNNNTTC 1 cut(s) 433
XspI CTAG 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.