Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr12

Orthogroup ID

OG0017976

Physical Location & Seq

Genomic Coordinates Forward (+)

1140514 .. 1142104

Gene Structure 1591 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 630 bp

ATGCCCGTCTCTTCAGTCTCCCGACCAAGGCTCTTACCAACTCTCTCTCATCTCACTTTCTCGACACACTCTCTCACTTCTCTCTCTCTGCATCCATCCATGGCGACGATCGATCAGAGCATTAAGGCTAGGCAGATCTTCGACAGCCGTGGCAATCCCACCGTCGAGGTCGATATCATACTGTCCGATGGCACTTTAGCCAGAGCCGCCGTTCCAAGTGGTGCATCCACCGGAGTTTACGAGGCCCTTGAGTTGCGCGATGGAGGTAAAGACTACCTCGGAAAGGGAGTATCGAAGGCTGTGAACAATGTCAACTCAATCATCGGCCCTGCTTTGATCGGCAAGGACCCAAGTGAACAGACTGCTATTGACAACTTCATGGTTCAACAGCTCGATGGAACTGTTAACGAGTGGGGTTGGTGCAAACAAAAGCTTGGTGCAAATGCCATTTTGGCCGTGTCTCTTGCAGTCGCCAAGGCTGGCGCTAGCGTCAAGAAAATTCCTCTGTACAAGCATATCGCCAATCTTGCTGGCAACAAGAACTTAGTTTTGCCCGTTCCTGCTTTCAATGTCATCAACGGTGGATCGATCGACATTCAGGAAACAAGCTTGCAATGCAGGAGTTCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001101 GO:0003674 GO:0003676 GO:0003677 GO:0003824 GO:0004634 GO:0005488 GO:0005507 GO:0005575 GO:0005576 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005739 GO:0005740 GO:0005829 GO:0005886 GO:0005911 GO:0006950 GO:0006970 GO:0008150 GO:0009266 GO:0009314 GO:0009409 GO:0009416 GO:0009506 GO:0009507 GO:0009536 GO:0009628 GO:0009651 GO:0009719 GO:0009725 GO:0009737 GO:0010033 GO:0010035 GO:0010038 GO:0016020 GO:0016829 GO:0016835 GO:0016836 GO:0030054 GO:0031967 GO:0031975 GO:0033993 GO:0042221 GO:0043167 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0046686 GO:0046872 GO:0046914 GO:0048046 GO:0050896 GO:0055044 GO:0071944 GO:0097159 GO:0097305 GO:1901363 GO:1901700

KEGG Pathways

Metabolic & Signaling

ko00010 ko00680 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko03018 ko04066 map00010 map00680 map01100 map01110 map01120 map01130 map01200 map01230 map03018 map04066

Pfam Domains

Protein Families

Enolase_C Enolase_N

Protein Analysis

210

Amino Acids

22.01

Weight (kDa)

8.91

Isoelectric Point (pI)

36.9

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Enolase_N	PF03952	39 - 173	2.1e-53	Enolase, N-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0017976)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g30481 FvH4_1g30560 FvH4_c4g00030
pyrus_communis	pycom05g12520 pycom12g01350
rosa_chinensis	RchiOBHm_Chr7g0214251
rosa_multiflora	Rmu_sc0000410.1_g000007 Rmu_sc0001154.1_g000014
rosa_roxburghii	Rroxscaffold_4G00313870 Rroxscaffold_4G00323230 Rroxscaffold_5G00382810

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	258
AciI	CCGC	1 cut(s)	207
AclWI	GGATC	1 cut(s)	592
AcoI	YGGCCR	1 cut(s)	453
AcsI	RAATTY	1 cut(s)	498
AfaI	GTAC	1 cut(s)	509
AfiI	CCNNNNNNNGG	2 cut(s)	27, 283
AgsI	TTSAA	2 cut(s)	386, 568
AluBI	AGCT	3 cut(s)	391, 433, 609
AluI	AGCT	3 cut(s)	391, 433, 609
Alw26I	GTCTC	3 cut(s)	13, 22, 465
AlwI	GGATC	1 cut(s)	592
AoxI	GGCC	3 cut(s)	243, 325, 453
ApoI	RAATTY	1 cut(s)	498
AspLEI	GCGC	2 cut(s)	258, 485
AspS9I	GGNCC	3 cut(s)	244, 326, 346
AsuNHI	GCTAGC	1 cut(s)	485
AvaII	GGWCC	1 cut(s)	346
BccI	CCATC	4 cut(s)	103, 182, 254, 389
BceAI	ACGGC	3 cut(s)	132, 194, 440
BcoDI	GTCTC	3 cut(s)	13, 22, 465
BfaI	CTAG	2 cut(s)	129, 486
BfoI	RGCGCY	1 cut(s)	486
BglI	GCCNNNNNGGC	1 cut(s)	452
BglII	AGATCT	1 cut(s)	135
BisI	GCNGC	1 cut(s)	207
BlsI	GCNGC	1 cut(s)	208
Bme18I	GGWCC	1 cut(s)	346
BmgT120I	GGNCC	3 cut(s)	244, 326, 346
BmiI	GGNNCC	1 cut(s)	348
BmsI	GCATC	2 cut(s)	100, 233
BmtI	GCTAGC	1 cut(s)	489
BpuEI	CTTGAG	1 cut(s)	269
Bsa29I	ATCGAT	2 cut(s)	111, 587
BsaJI	CCNNGG	5 cut(s)	26, 99, 148, 277, 474
BsaWI	WCCGGW	1 cut(s)	230
Bsc4I	CCNNNNNNNGG	2 cut(s)	27, 283
Bse3DI	GCAATG	1 cut(s)	620
BseCI	ATCGAT	2 cut(s)	111, 587
BseDI	CCNNGG	5 cut(s)	26, 99, 148, 277, 474
BseGI	GGATG	3 cut(s)	91, 95, 224
BseLI	CCNNNNNNNGG	2 cut(s)	27, 283
BseMI	GCAATG	1 cut(s)	620
Bsh1236I	CGCG	1 cut(s)	258
Bsh1285I	CGRYCG	2 cut(s)	111, 591
BshFI	GGCC	3 cut(s)	245, 327, 455
BshVI	ATCGAT	2 cut(s)	111, 587
BsiEI	CGRYCG	2 cut(s)	111, 591
BsiSI	CCGG	1 cut(s)	231
BslI	CCNNNNNNNGG	2 cut(s)	27, 283
BsmAI	GTCTC	3 cut(s)	13, 22, 465
BsmBI	CGTCTC	1 cut(s)	13
BsnI	GGCC	3 cut(s)	245, 327, 455
Bsp1407I	TGTACA	1 cut(s)	507
Bsp143I	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
Bsp19I	CCATGG	1 cut(s)	99
BspACI	CCGC	1 cut(s)	207
BspANI	GGCC	3 cut(s)	245, 327, 455
BspDI	ATCGAT	2 cut(s)	111, 587
BspFNI	CGCG	1 cut(s)	258
BspHI	TCATGA	1 cut(s)	626
BspLI	GGNNCC	1 cut(s)	348
BspOI	GCTAGC	1 cut(s)	489
BspPI	GGATC	1 cut(s)	592
BsrDI	GCAATG	1 cut(s)	620
BsrGI	TGTACA	1 cut(s)	507
BssECI	CCNNGG	5 cut(s)	26, 99, 148, 277, 474
BssMI	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
BssT1I	CCWWGG	3 cut(s)	26, 99, 474
Bst4CI	ACNGT	4 cut(s)	163, 183, 403, 581
Bst6I	CTCTTC	1 cut(s)	16
BstAUI	TGTACA	1 cut(s)	507
BstC8I	GCNNGC	4 cut(s)	481, 487, 532, 611
BstDEI	CTNAG	1 cut(s)	544
BstDSI	CCRYGG	2 cut(s)	99, 148
BstENI	CCTNNNNNAGG	1 cut(s)	281
BstF5I	GGATG	3 cut(s)	91, 95, 224
BstFNI	CGCG	1 cut(s)	258
BstH2I	RGCGCY	1 cut(s)	486
BstHHI	GCGC	2 cut(s)	258, 485
BstKTI	GATC	6 cut(s)	111, 115, 138, 339, 587, 591
BstMAI	GTCTC	3 cut(s)	13, 22, 465
BstMBI	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
BstMCI	CGRYCG	2 cut(s)	111, 591
BstMWI	GCNNNNNNNGC	4 cut(s)	206, 452, 527, 615
BstUI	CGCG	1 cut(s)	258
BstX2I	RGATCY	1 cut(s)	135
BstYI	RGATCY	1 cut(s)	135
Bsu15I	ATCGAT	2 cut(s)	111, 587
BsuRI	GGCC	3 cut(s)	245, 327, 455
BsuTUI	ATCGAT	2 cut(s)	111, 587
BtgI	CCRYGG	2 cut(s)	99, 148
BtgZI	GCGATG	1 cut(s)	273
BtsCI	GGATG	3 cut(s)	91, 95, 224
Cac8I	GCNNGC	4 cut(s)	481, 487, 532, 611
CciI	TCATGA	1 cut(s)	626
CfoI	GCGC	2 cut(s)	258, 485
Cfr13I	GGNCC	3 cut(s)	244, 326, 346
ClaI	ATCGAT	2 cut(s)	111, 587
CseI	GACGC	1 cut(s)	478
Csp6I	GTAC	1 cut(s)	508
CviAII	CATG	3 cut(s)	100, 379, 627
CviQI	GTAC	1 cut(s)	508
DdeI	CTNAG	1 cut(s)	544
DpnI	GATC	6 cut(s)	110, 114, 137, 338, 586, 590
DpnII	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
EaeI	YGGCCR	1 cut(s)	453
Eam1104I	CTCTTC	1 cut(s)	16
EarI	CTCTTC	1 cut(s)	16
Eco130I	CCWWGG	3 cut(s)	26, 99, 474
Eco32I	GATATC	1 cut(s)	175
Eco47I	GGWCC	1 cut(s)	346
EcoNI	CCTNNNNNAGG	1 cut(s)	281
EcoO109I	RGGNCCY	2 cut(s)	244, 346
EcoRV	GATATC	1 cut(s)	175
EcoT14I	CCWWGG	3 cut(s)	26, 99, 474
ErhI	CCWWGG	3 cut(s)	26, 99, 474
Esp3I	CGTCTC	1 cut(s)	13
FaeI	CATG	3 cut(s)	103, 382, 630
FaiI	YATR	5 cut(s)	101, 179, 380, 516, 628
FatI	CATG	3 cut(s)	99, 378, 626
Fnu4HI	GCNGC	1 cut(s)	207
FokI	GGATG	3 cut(s)	78, 82, 211
Fsp4HI	GCNGC	1 cut(s)	207
FspBI	CTAG	2 cut(s)	129, 486
GlaI	GCGC	2 cut(s)	257, 484
GluI	GCNGC	1 cut(s)	207
HaeII	RGCGCY	1 cut(s)	486
HaeIII	GGCC	3 cut(s)	245, 327, 455
HapII	CCGG	1 cut(s)	231
HgaI	GACGC	1 cut(s)	478
HhaI	GCGC	2 cut(s)	258, 485
Hin1II	CATG	3 cut(s)	103, 382, 630
Hin6I	GCGC	2 cut(s)	256, 483
HinP1I	GCGC	2 cut(s)	256, 483
HincII	GTYRAC	2 cut(s)	313, 406
HindII	GTYRAC	2 cut(s)	313, 406
HindIII	AAGCTT	2 cut(s)	431, 607
HpaI	GTTAAC	1 cut(s)	406
HpaII	CCGG	1 cut(s)	231
Hpy166II	GTNNAC	5 cut(s)	238, 304, 313, 356, 406
Hpy188I	TCNGA	3 cut(s)	117, 187, 281
Hpy188III	TCNNGA	5 cut(s)	21, 61, 493, 599, 627
Hpy8I	GTNNAC	5 cut(s)	238, 304, 313, 356, 406
Hpy99I	CGWCG	2 cut(s)	109, 167
HpyAV	CCTTC	1 cut(s)	289
HpyCH4III	ACNGT	4 cut(s)	163, 183, 403, 581
HpyCH4V	TGCA	7 cut(s)	91, 224, 423, 440, 467, 613, 618
HpyF10VI	GCNNNNNNNGC	4 cut(s)	206, 452, 527, 615
HpyF3I	CTNAG	1 cut(s)	544
Hsp92II	CATG	3 cut(s)	103, 382, 630
HspAI	GCGC	2 cut(s)	256, 483
KspAI	GTTAAC	1 cut(s)	406
Kzo9I	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
LpnPI	CCDG	8 cut(s)	214, 244, 342, 465, 516, 573, 584, 604
LweI	GCATC	2 cut(s)	100, 233
MaeI	CTAG	2 cut(s)	129, 486
MalI	GATC	6 cut(s)	110, 114, 137, 338, 586, 590
MboI	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
MboII	GAAGA	2 cut(s)	3, 130
MflI	RGATCY	1 cut(s)	135
MluCI	AATT	1 cut(s)	498
MnlI	CCTC	5 cut(s)	160, 235, 257, 287, 513
MseI	TTAA	2 cut(s)	123, 405
MspI	CCGG	1 cut(s)	231
MvnI	CGCG	1 cut(s)	258
MwoI	GCNNNNNNNGC	4 cut(s)	206, 452, 527, 615
NcoI	CCATGG	1 cut(s)	99
NdeII	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
NheI	GCTAGC	1 cut(s)	485
NlaIII	CATG	3 cut(s)	103, 382, 630
NlaIV	GGNNCC	1 cut(s)	348
PagI	TCATGA	1 cut(s)	626
PkrI	GCNGC	1 cut(s)	208
Ple19I	CGATCG	2 cut(s)	111, 591
PpuMI	RGGWCCY	1 cut(s)	346
Psp5II	RGGWCCY	1 cut(s)	346
PspN4I	GGNNCC	1 cut(s)	348
PspPI	GGNCC	3 cut(s)	244, 326, 346
PspPPI	RGGWCCY	1 cut(s)	346
PsuI	RGATCY	1 cut(s)	135
PvuI	CGATCG	2 cut(s)	111, 591
RsaI	GTAC	1 cut(s)	509
RsaNI	GTAC	1 cut(s)	508
SaqAI	TTAA	2 cut(s)	123, 405
SatI	GCNGC	1 cut(s)	207
Sau3AI	GATC	6 cut(s)	108, 112, 135, 336, 584, 588
Sau96I	GGNCC	3 cut(s)	244, 326, 346
SetI	ASST	6 cut(s)	171, 268, 279, 393, 435, 611
SfaNI	GCATC	2 cut(s)	100, 233
SinI	GGWCC	1 cut(s)	346
SmlI	CTYRAG	1 cut(s)	248
SmoI	CTYRAG	1 cut(s)	248
Sse9I	AATT	1 cut(s)	498
SsiI	CCGC	1 cut(s)	207
SspMI	CTAG	2 cut(s)	129, 486
StyI	CCWWGG	3 cut(s)	26, 99, 474
TaaI	ACNGT	4 cut(s)	163, 183, 403, 581
TaqI	TCGA	9 cut(s)	62, 111, 141, 165, 171, 293, 393, 587, 591
TasI	AATT	1 cut(s)	498
TatI	WGTACW	1 cut(s)	507
TauI	GCSGC	1 cut(s)	209
Tru1I	TTAA	2 cut(s)	123, 405
Tru9I	TTAA	2 cut(s)	123, 405
TspDTI	ATGAA	2 cut(s)	367, 615
VpaK11BI	GGWCC	1 cut(s)	346
XagI	CCTNNNNNAGG	1 cut(s)	281
XapI	RAATTY	1 cut(s)	498
XspI	CTAG	2 cut(s)	129, 486

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.