pycom12g02430

Bifunctional monodehydroascorbate reductase and carbonic anhydrase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0010395
Physical Location & Seq
Forward (+)
2109266 .. 2114784
5519 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 753 bp
ATGGCCCTTAGAGTTTCCTTCTCCTTCCTTGTGATAGCCTTGCTGCTCGTCTTCGGCCCTTCGGCTTCTGTCCAAGATGGTTTATCTACGTGCATGCAGACACACTCTCATTTAGCTACACTGGTCAAACTGGCTCAAATGGGAAGATGCAGTCTCCGGTTGACGTCGTCAAGATCTACCGTACTTCAAATGCCACATTGTTTAACAACGGCTTTAACCTTGGGGAAGTTGCGTTTCGAAGGAAATGGGGGAACACTAGATGTGGATGGTAAGAACTACAACTTGATGCAGTTGCACTGGCACACTCCCTCTGAGCATCGCCTTAATGGAATCCAATTTCCAGCGGAGCTTCATCTGGTTCACCAGGCAGCTGATCAAAGCTTCGCAGTTGTGGCGATCCTCTTAAATTTCGGCAAGGAAGATATCATCCTCTTACAGATTAAGAACAAGCTGGCCGAGCTGGCCAAGGAGGCGTGCAAGAAAGATGAAGATGCTATCATTCCCGTTGGAACTGTTGATCTGAACGAGTTACAGAAGAAGAGTCGCAAATATTACAGATACGTTGGCTCTCTCACCGTTCCTCCATGCACCGAAAATGTTGTCTGGAGCATTCTCGGAAAGGTGAGGACTATTTCCAAACATCAGTTAGATGCACTAAAAGCACCATTGGATGCATCTTGCAAAAACAACTCAAGACCTCTTCAATCTCCAAATGGACGCAAGATTGAGCTCTACGATGAGCTTATGCACTAG

Protein Analysis

251

Amino Acids

27.74

Weight (kDa)

9.0

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Carb_anhydrase PF00194 78 - 243 6.7e-29 Eukaryotic-type carbonic anhydrase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0010395)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52720 AT3G52720 AT3G52720 AT3G52720
fragaria_vesca FvH4_6g18830 FvH4_6g18830
malus_domestica MD12G1024600.v1.1
prunus_persica Prupe.7G109300_v2.0.a1 Prupe.7G109300_v2.0.a1
pyrus_communis pycom12g02430
rosa_chinensis RchiOBHm_Chr3g0473841
rosa_laevigata RLG00000023979
rosa_multiflora Rmu_sc0005561.1_g000003
rosa_roxburghii Rroxscaffold_6G00407730
rosa_rugosa Rorug03G0135900 Rorug03G0136000
rosa_samantha Rh3AG186400 Rh3BG215000 Rh3CG211700 Rh3DG210800
rosa_wichuraiana Rw3G017050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 167
AciI CCGC 1 cut(s) 344
AclWI GGATC 1 cut(s) 391
AcoI YGGCCR 2 cut(s) 453, 462
AcsI RAATTY 1 cut(s) 406
AcyI GRCGYC 1 cut(s) 164
AfaI GTAC 1 cut(s) 183
AgsI TTSAA 2 cut(s) 188, 704
AjnI CCWGG 1 cut(s) 363
AluBI AGCT 8 cut(s) 116, 349, 371, 381, 451, 460, 730, 742
AluI AGCT 8 cut(s) 116, 349, 371, 381, 451, 460, 730, 742
Alw21I GWGCWC 1 cut(s) 732
Alw26I GTCTC 1 cut(s) 158
AlwI GGATC 1 cut(s) 391
AoxI GGCC 4 cut(s) 3, 55, 453, 462
ApeKI GCWGC 2 cut(s) 43, 368
ApoI RAATTY 1 cut(s) 406
ArsI GACNNNNNNTTYG 2 cut(s) 585, 617
AspS9I GGNCC 2 cut(s) 4, 56
AsuHPI GGTGA 3 cut(s) 353, 565, 634
AsuII TTCGAA 1 cut(s) 237
BalI TGGCCA 1 cut(s) 464
BanII GRGCYC 1 cut(s) 732
BbsI GAAGAC 1 cut(s) 43
Bbv12I GWGCWC 1 cut(s) 732
BbvI GCAGC 2 cut(s) 30, 380
BccI CCATC 2 cut(s) 71, 260
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 365
BclI TGATCA 1 cut(s) 373
BcoDI GTCTC 1 cut(s) 158
BfaI CTAG 2 cut(s) 257, 751
BglI GCCNNNNNGGC 2 cut(s) 461, 470
BglII AGATCT 1 cut(s) 173
BisI GCNGC 2 cut(s) 44, 369
BlsI GCNGC 2 cut(s) 45, 370
Bme1390I CCNGG 1 cut(s) 365
BmgT120I GGNCC 2 cut(s) 4, 56
BmrFI CCNGG 1 cut(s) 365
BmsI GCATC 7 cut(s) 137, 276, 325, 481, 640, 661, 683
BpiI GAAGAC 1 cut(s) 43
BpmI CTGGAG 1 cut(s) 625
Bpu14I TTCGAA 1 cut(s) 237
BpuEI CTTGAG 1 cut(s) 676
BsaAI YACGTR 1 cut(s) 90
BsaHI GRCGYC 1 cut(s) 164
BsaJI CCNNGG 2 cut(s) 219, 465
BsaWI WCCGGW 1 cut(s) 156
BsaXI ACNNNNNCTCC 2 cut(s) 565, 595
Bse1I ACTGG 3 cut(s) 126, 135, 302
BseBI CCWGG 1 cut(s) 365
BseDI CCNNGG 2 cut(s) 219, 465
BseGI GGATG 3 cut(s) 271, 426, 676
BseMII CTCAG 1 cut(s) 303
BseNI ACTGG 3 cut(s) 126, 135, 302
BseXI GCAGC 2 cut(s) 30, 380
BshFI GGCC 4 cut(s) 5, 57, 455, 464
BsiHKAI GWGCWC 1 cut(s) 732
BsiSI CCGG 1 cut(s) 157
BsmAI GTCTC 1 cut(s) 158
BsmI GAATGC 1 cut(s) 609
BsnI GGCC 4 cut(s) 5, 57, 455, 464
Bsp119I TTCGAA 1 cut(s) 237
Bsp1286I GDGCHC 1 cut(s) 732
Bsp143I GATC 4 cut(s) 173, 373, 396, 517
BspACI CCGC 1 cut(s) 344
BspANI GGCC 4 cut(s) 5, 57, 455, 464
BspCNI CTCAG 1 cut(s) 304
BspPI GGATC 1 cut(s) 391
BspT104I TTCGAA 1 cut(s) 237
BsrI ACTGG 3 cut(s) 126, 135, 302
BssECI CCNNGG 2 cut(s) 219, 465
BssMI GATC 4 cut(s) 173, 373, 396, 517
BssNI GRCGYC 1 cut(s) 164
BssT1I CCWWGG 2 cut(s) 219, 465
Bst2UI CCWGG 1 cut(s) 365
Bst4CI ACNGT 3 cut(s) 181, 514, 577
Bst6I CTCTTC 2 cut(s) 533, 705
BstACI GRCGYC 1 cut(s) 164
BstBAI YACGTR 1 cut(s) 90
BstBI TTCGAA 1 cut(s) 237
BstC8I GCNNGC 4 cut(s) 95, 453, 462, 475
BstDEI CTNAG 2 cut(s) 8, 312
BstF5I GGATG 3 cut(s) 271, 426, 676
BstKTI GATC 4 cut(s) 176, 376, 399, 520
BstMAI GTCTC 1 cut(s) 158
BstMBI GATC 4 cut(s) 173, 373, 396, 517
BstMWI GCNNNNNNNGC 5 cut(s) 392, 457, 461, 470, 659
BstNI CCWGG 1 cut(s) 365
BstNSI RCATGY 1 cut(s) 97
BstSCI CCNGG 1 cut(s) 363
BstV1I GCAGC 2 cut(s) 30, 380
BstV2I GAAGAC 1 cut(s) 43
BstX2I RGATCY 1 cut(s) 173
BstYI RGATCY 1 cut(s) 173
BsuRI GGCC 4 cut(s) 5, 57, 455, 464
BtgZI GCGATG 1 cut(s) 302
BtsCI GGATG 3 cut(s) 271, 426, 676
BtsIMutI CAGTG 2 cut(s) 119, 295
Cac8I GCNNGC 4 cut(s) 95, 453, 462, 475
Cfr13I GGNCC 2 cut(s) 4, 56
CseI GACGC 1 cut(s) 726
Csp6I GTAC 1 cut(s) 182
CviAII CATG 2 cut(s) 94, 585
CviQI GTAC 1 cut(s) 182
DdeI CTNAG 2 cut(s) 8, 312
DpnI GATC 4 cut(s) 175, 375, 398, 519
DpnII GATC 4 cut(s) 173, 373, 396, 517
EaeI YGGCCR 2 cut(s) 453, 462
Eam1104I CTCTTC 2 cut(s) 533, 705
EarI CTCTTC 2 cut(s) 533, 705
Ecl136II GAGCTC 1 cut(s) 730
Eco130I CCWWGG 2 cut(s) 219, 465
Eco24I GRGCYC 1 cut(s) 732
Eco32I GATATC 1 cut(s) 424
Eco53kI GAGCTC 1 cut(s) 730
EcoICRI GAGCTC 1 cut(s) 730
EcoRII CCWGG 1 cut(s) 363
EcoRV GATATC 1 cut(s) 424
EcoT14I CCWWGG 2 cut(s) 219, 465
EcoT22I ATGCAT 1 cut(s) 676
EcoT38I GRGCYC 1 cut(s) 732
ErhI CCWWGG 2 cut(s) 219, 465
FaeI CATG 2 cut(s) 97, 588
FaiI YATR 3 cut(s) 95, 586, 746
FatI CATG 2 cut(s) 93, 584
FbaI TGATCA 1 cut(s) 373
Fnu4HI GCNGC 2 cut(s) 44, 369
FokI GGATG 3 cut(s) 278, 413, 683
FriOI GRGCYC 1 cut(s) 732
Fsp4HI GCNGC 2 cut(s) 44, 369
FspBI CTAG 2 cut(s) 257, 751
GluI GCNGC 2 cut(s) 44, 369
GsuI CTGGAG 1 cut(s) 625
HaeIII GGCC 4 cut(s) 5, 57, 455, 464
HapII CCGG 1 cut(s) 157
HgaI GACGC 1 cut(s) 726
Hin1I GRCGYC 1 cut(s) 164
Hin1II CATG 2 cut(s) 97, 588
HincII GTYRAC 1 cut(s) 162
HindII GTYRAC 1 cut(s) 162
HindIII AAGCTT 1 cut(s) 379
HinfI GANTC 2 cut(s) 330, 541
HpaII CCGG 1 cut(s) 157
HphI GGTGA 3 cut(s) 353, 565, 634
Hpy166II GTNNAC 2 cut(s) 162, 361
Hpy188I TCNGA 3 cut(s) 313, 522, 617
Hpy188III TCNNGA 3 cut(s) 171, 604, 693
Hpy8I GTNNAC 2 cut(s) 162, 361
Hpy99I CGWCG 1 cut(s) 169
HpyAV CCTTC 4 cut(s) 28, 34, 69, 233
HpyCH4III ACNGT 3 cut(s) 181, 514, 577
HpyCH4IV ACGT 3 cut(s) 89, 164, 561
HpyF10VI GCNNNNNNNGC 5 cut(s) 392, 457, 461, 470, 659
HpyF3I CTNAG 2 cut(s) 8, 312
HpySE526I ACGT 3 cut(s) 89, 164, 561
Hsp92I GRCGYC 1 cut(s) 164
Hsp92II CATG 2 cut(s) 97, 588
Ksp22I TGATCA 1 cut(s) 373
Kzo9I GATC 4 cut(s) 173, 373, 396, 517
LmnI GCTCC 2 cut(s) 346, 606
Lsp1109I GCAGC 2 cut(s) 30, 380
LweI GCATC 7 cut(s) 137, 276, 325, 481, 640, 661, 683
MaeI CTAG 2 cut(s) 257, 751
MaeII ACGT 3 cut(s) 89, 164, 561
MaeIII GTNAC 1 cut(s) 528
MalI GATC 4 cut(s) 175, 375, 398, 519
MboI GATC 4 cut(s) 173, 373, 396, 517
MboII GAAGA 7 cut(s) 43, 156, 431, 500, 547, 550, 692
MflI RGATCY 1 cut(s) 173
MhlI GDGCHC 1 cut(s) 732
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 2 cut(s) 335, 406
MluNI TGGCCA 1 cut(s) 464
MlyI GAGTC 1 cut(s) 550
MmeI TCCRAC 1 cut(s) 487
MnlI CCTC 7 cut(s) 319, 410, 440, 463, 591, 618, 708
Mox20I TGGCCA 1 cut(s) 464
Mph1103I ATGCAT 1 cut(s) 676
MscI TGGCCA 1 cut(s) 464
MseI TTAA 5 cut(s) 203, 215, 324, 404, 441
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 2 cut(s) 344, 371
MspI CCGG 1 cut(s) 157
MspR9I CCNGG 1 cut(s) 365
Mva1269I GAATGC 1 cut(s) 609
MvaI CCWGG 1 cut(s) 365
MwoI GCNNNNNNNGC 5 cut(s) 392, 457, 461, 470, 659
NdeII GATC 4 cut(s) 173, 373, 396, 517
NlaIII CATG 2 cut(s) 97, 588
NmeAIII GCCGAG 1 cut(s) 481
NsiI ATGCAT 1 cut(s) 676
NspI RCATGY 1 cut(s) 97
NspV TTCGAA 1 cut(s) 237
PaeI GCATGC 1 cut(s) 97
PctI GAATGC 1 cut(s) 609
PfeI GAWTC 1 cut(s) 330
PflFI GACNNNGTC 1 cut(s) 166
PkrI GCNGC 2 cut(s) 45, 370
PleI GAGTC 1 cut(s) 549
PpsI GAGTC 1 cut(s) 549
Ppu21I YACGTR 1 cut(s) 90
Psp124BI GAGCTC 1 cut(s) 732
Psp6I CCWGG 1 cut(s) 363
PspGI CCWGG 1 cut(s) 363
PspPI GGNCC 2 cut(s) 4, 56
PsuI RGATCY 1 cut(s) 173
PsyI GACNNNGTC 1 cut(s) 166
PvuII CAGCTG 1 cut(s) 371
RsaI GTAC 1 cut(s) 183
RsaNI GTAC 1 cut(s) 182
SacI GAGCTC 1 cut(s) 732
SaqAI TTAA 5 cut(s) 203, 215, 324, 404, 441
SatI GCNGC 2 cut(s) 44, 369
Sau3AI GATC 4 cut(s) 173, 373, 396, 517
Sau96I GGNCC 2 cut(s) 4, 56
SchI GAGTC 1 cut(s) 550
ScrFI CCNGG 1 cut(s) 365
SduI GDGCHC 1 cut(s) 732
SfaNI GCATC 7 cut(s) 137, 276, 325, 481, 640, 661, 683
SfiI GGCCNNNNNGGCC 1 cut(s) 461
SfuI TTCGAA 1 cut(s) 237
SmlI CTYRAG 1 cut(s) 691
SmoI CTYRAG 1 cut(s) 691
SphI GCATGC 1 cut(s) 97
Sse9I AATT 2 cut(s) 335, 406
SsiI CCGC 1 cut(s) 344
SspI AATATT 1 cut(s) 551
SspMI CTAG 2 cut(s) 257, 751
SstI GAGCTC 1 cut(s) 732
StyD4I CCNGG 1 cut(s) 363
StyI CCWWGG 2 cut(s) 219, 465
TaaI ACNGT 3 cut(s) 181, 514, 577
TaiI ACGT 3 cut(s) 92, 167, 564
TaqI TCGA 1 cut(s) 237
TasI AATT 2 cut(s) 335, 406
TfiI GAWTC 1 cut(s) 330
Tru1I TTAA 5 cut(s) 203, 215, 324, 404, 441
Tru9I TTAA 5 cut(s) 203, 215, 324, 404, 441
TscAI CASTG 2 cut(s) 126, 302
TseI GCWGC 2 cut(s) 43, 368
TspDTI ATGAA 2 cut(s) 341, 501
TspRI CASTG 2 cut(s) 126, 302
Tth111I GACNNNGTC 1 cut(s) 166
XapI RAATTY 1 cut(s) 406
XceI RCATGY 1 cut(s) 97
XspI CTAG 2 cut(s) 257, 751
ZraI GACGTC 1 cut(s) 165
Zsp2I ATGCAT 1 cut(s) 676
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.