pycom12g02650

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0014060
Physical Location & Seq
Reverse (-)
2381184 .. 2384073
2890 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g02650.2

Sequence Viewer

Length: 1434 bp
ATGTCGGAGTCGGATGATCAAAACCCCAACCCACCAGCAATGCCGGAGCCCAAGACCCGACCCGTCGGCGGGACTGAGCTCAGCTGGTGCAAAGCCGTTCCTAGCGGCACCGGCATAACAGTCCTAGCCCTCCTCCTCTCGAGACCCCCAAACTTCTCAAATCTCCAAACTGCCCTCCACAACCTCCAAAATTCCCACCCCATCCTCCGCTCGAAACACCACTTCGACCCCGCCACCGGTACCTACTCCTTCCTCACTCCCCCCACCCCTCACCTCCAAATCCAGCCGTTCGATCTCCCATCAACGGCTCCCATTCTCCAAATCAACCCCGACAATATCGCCCCGTTGCATCAGATCCTTGAGCACGAGATGAACTTGAACACGTGGCAAAACCTCGACCCCTCGTTGGAATCCGACACGGACGTGATATATGCGAGCACGTACGCGATAAGCGAGTCGCGGTGGGTACTGGTGCTCCGCCTGCACACGTCAGCATGCGACCGAGCGGCGGCCGTGGCCGTGCTGAAGGAGCTGCTGCGGGAGTTGAAGTCAACGGGGGGAGGAGGGGCAGAGAAGGAATTAAAGGGAGACGAGGAGGTGAGTTTGGGAATAGAAGATTTGATCCCGAACGGGAAGGCGAATAAGCCGTTTTGGGCGCGTGGGGTGGACGTGCTGGGGTACTCTTTGAATTCGCTGAGGCTGTCGAACCTGGAGTTCCAAGACGTGAGTTCAGAAAGGCGCTCCCAGGTGGTGAAATTACAGTTGAGCCCAGAAGATACCGACAGGCTGCTCGCTGGTTGTAAGTCTAGAGATATAAAATTATGCGGAGCCTTGGCAGCTGCTGGAATGATTGCTGCCCGTGCCTCTAAGCAGCTTCCCGATCATCAATGGGAGAAGTATGGTGTTGTAACCCTACTCGATTGTCGGCCAATTCTTGATCCACCCCTCTCTATCAATCATCTCGGGTTTTATCATTCTGCCATCATGAACACGCATGACATCAACGGAGAAGGCAATACATTGTGGGATCTGGCAAAGAGATGCTACACGTCCTTTGCCGGTGCCAAGAACTCTAACAAGCATTTCACGGACATGGCAGACCTCAACTTTCTCATGTGCAAGGCTATTGAAAACCCTGGTTTGACCCCATCATCGGCAATGAGAACTGCCTTTATCTCAGTGTTTGAGGACACCGTAATTGATGAGTCTAGTGAAGTCAACAAAGAGCTTGGGGTTGAGGACTATGAGGGTTGCGCTTCAGTCCATGGAGTTGGCCCCTCCATAGCCATTTTCGACACCATACGTGATGGATGCTTGAACTGTGCTTGTGTTTATCCGTCTCCATTGCATTCGAAGGAACAAATGCAGGACTTGATGGACCGCATGAAGAGCATTCTTGTGGACGGTTGTAAGGATGTTGAGAGTGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AATase

Protein Analysis

478

Amino Acids

52.25

Weight (kDa)

5.16

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014060)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G52610
fragaria_vesca FvH4_6g19190
malus_domestica MD12G1027700.v1.1
prunus_persica Prupe.7G103700_v2.0.a1 Prupe.7G103700_v2.0.a1 Prupe.7G103900_v2.0.a1
pyrus_communis pycom12g02650
rosa_chinensis RchiOBHm_Chr3g0474311
rosa_laevigata RLG00000023933
rosa_multiflora Rmu_sc0004507.1_g000012
rosa_roxburghii Rroxscaffold_6G00407370
rosa_rugosa Rorug03G0140000
rosa_samantha Rh3AG190700 Rh3BG219900 Rh3CG215100 Rh3DG215600
rosa_wichuraiana Rw3G017400

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 239
AccB1I GGYRCC 3 cut(s) 107, 239, 1061
AccBSI CCGCTC 2 cut(s) 210, 506
AccII CGCG 3 cut(s) 446, 460, 658
AclWI GGATC 4 cut(s) 349, 616, 932, 1035
AcoI YGGCCR 3 cut(s) 510, 516, 926
AcsI RAATTY 2 cut(s) 190, 688
AcuI CTGAAG 2 cut(s) 545, 1242
AcvI CACGTG 1 cut(s) 384
AfaI GTAC 4 cut(s) 241, 443, 468, 680
AfiI CCNNNNNNNGG 7 cut(s) 68, 69, 236, 304, 406, 508, 1153
AflIII ACRYGT 3 cut(s) 381, 486, 1047
AgeI ACCGGT 1 cut(s) 236
AgsI TTSAA 5 cut(s) 379, 547, 688, 1130, 1318
AjiI CACGTC 5 cut(s) 424, 489, 670, 724, 1050
AjnI CCWGG 3 cut(s) 708, 744, 1135
AleI CACNNNNGTG 1 cut(s) 422
AluBI AGCT 7 cut(s) 79, 84, 532, 839, 874, 1228, 1431
AluI AGCT 7 cut(s) 79, 84, 532, 839, 874, 1228, 1431
Alw21I GWGCWC 4 cut(s) 81, 366, 440, 477
Alw26I GTCTC 3 cut(s) 136, 582, 1344
AlwI GGATC 4 cut(s) 349, 616, 932, 1035
AlwNI CAGNNNCTG 1 cut(s) 842
Ama87I CYCGRG 2 cut(s) 139, 962
AoxI GGCC 4 cut(s) 510, 516, 926, 1273
ApeKI GCWGC 7 cut(s) 532, 535, 787, 836, 839, 854, 871
ApoI RAATTY 2 cut(s) 190, 688
AsiGI ACCGGT 1 cut(s) 236
Asp718I GGTACC 1 cut(s) 239
AspLEI GCGC 3 cut(s) 658, 741, 1256
AspS9I GGNCC 2 cut(s) 1274, 1378
AsuHPI GGTGA 3 cut(s) 263, 610, 763
AsuII TTCGAA 1 cut(s) 1352
AvaI CYCGRG 2 cut(s) 139, 962
AvaII GGWCC 1 cut(s) 1378
BanI GGYRCC 3 cut(s) 107, 239, 1061
BanII GRGCYC 3 cut(s) 51, 81, 770
BauI CACGAG 1 cut(s) 365
BbrPI CACGTG 1 cut(s) 384
Bbv12I GWGCWC 4 cut(s) 81, 366, 440, 477
BbvCI CCTCAGC 1 cut(s) 695
BbvI GCAGC 7 cut(s) 519, 522, 774, 826, 841, 848, 883
BccI CCATC 6 cut(s) 209, 307, 989, 1156, 1301, 1369
BceAI ACGGC 6 cut(s) 80, 271, 321, 497, 503, 631
BciT130I CCWGG 3 cut(s) 710, 746, 1137
BclI TGATCA 1 cut(s) 16
BcoDI GTCTC 3 cut(s) 136, 582, 1344
BfaI CTAG 5 cut(s) 102, 125, 807, 1209, 1432
BfoI RGCGCY 1 cut(s) 742
BlpI GCTNAGC 1 cut(s) 80
Bme1390I CCNGG 3 cut(s) 710, 746, 1137
Bme18I GGWCC 1 cut(s) 1378
BmeT110I CYCGRG 2 cut(s) 139, 962
BmgBI CACGTC 5 cut(s) 424, 489, 670, 724, 1050
BmgT120I GGNCC 2 cut(s) 1274, 1378
BmiI GGNNCC 7 cut(s) 48, 109, 241, 309, 829, 1063, 1276
BmrFI CCNGG 3 cut(s) 710, 746, 1137
BmsI GCATC 3 cut(s) 358, 1031, 1301
BpmI CTGGAG 1 cut(s) 731
Bpu10I CCTNAGC 1 cut(s) 695
Bpu1102I GCTNAGC 1 cut(s) 80
Bpu14I TTCGAA 1 cut(s) 1352
BpuEI CTTGAG 1 cut(s) 380
BsaAI YACGTR 3 cut(s) 384, 441, 1304
BsaI GGTCTC 1 cut(s) 136
BsaJI CCNNGG 5 cut(s) 513, 744, 831, 1135, 1264
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 4 cut(s) 459, 489, 533, 563
Bsc4I CCNNNNNNNGG 7 cut(s) 68, 69, 236, 304, 406, 508, 1153
Bse118I RCCGGY 3 cut(s) 110, 236, 1058
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 3 cut(s) 45, 1164, 1343
BseBI CCWGG 3 cut(s) 710, 746, 1137
BseDI CCNNGG 5 cut(s) 513, 744, 831, 1135, 1264
BseGI GGATG 4 cut(s) 19, 201, 1316, 1420
BseLI CCNNNNNNNGG 7 cut(s) 68, 69, 236, 304, 406, 508, 1153
BseMI GCAATG 3 cut(s) 45, 1164, 1343
BseMII CTCAG 4 cut(s) 66, 94, 686, 1191
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 4 cut(s) 122, 125, 576, 608
BseX3I CGGCCG 1 cut(s) 510
BseXI GCAGC 7 cut(s) 519, 522, 774, 826, 841, 848, 883
BseYI CCCAGC 1 cut(s) 673
BsgI GTGCAG 1 cut(s) 467
Bsh1236I CGCG 3 cut(s) 446, 460, 658
Bsh1285I CGRYCG 2 cut(s) 502, 513
BshFI GGCC 4 cut(s) 512, 518, 928, 1275
BshNI GGYRCC 3 cut(s) 107, 239, 1061
BshTI ACCGGT 1 cut(s) 236
BsiEI CGRYCG 2 cut(s) 502, 513
BsiHKAI GWGCWC 4 cut(s) 81, 366, 440, 477
BsiHKCI CYCGRG 2 cut(s) 139, 962
BsiSI CCGG 4 cut(s) 44, 111, 237, 1059
BsiWI CGTACG 1 cut(s) 441
BslFI GGGAC 1 cut(s) 85
BslI CCNNNNNNNGG 7 cut(s) 68, 69, 236, 304, 406, 508, 1153
BsmAI GTCTC 3 cut(s) 136, 582, 1344
BsmBI CGTCTC 2 cut(s) 582, 1344
BsmFI GGGAC 1 cut(s) 85
BsmI GAATGC 2 cut(s) 1348, 1392
BsnI GGCC 4 cut(s) 512, 518, 928, 1275
Bso31I GGTCTC 1 cut(s) 136
BsoBI CYCGRG 2 cut(s) 139, 962
Bsp119I TTCGAA 1 cut(s) 1352
Bsp1286I GDGCHC 6 cut(s) 51, 81, 366, 440, 477, 770
Bsp143I GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
Bsp1720I GCTNAGC 1 cut(s) 80
Bsp19I CCATGG 1 cut(s) 1264
BspANI GGCC 4 cut(s) 512, 518, 928, 1275
BspCNI CTCAG 4 cut(s) 67, 93, 687, 1190
BspFNI CGCG 3 cut(s) 446, 460, 658
BspHI TCATGA 1 cut(s) 984
BspLI GGNNCC 7 cut(s) 48, 109, 241, 309, 829, 1063, 1276
BspPI GGATC 4 cut(s) 349, 616, 932, 1035
BspQI GCTCTTC 1 cut(s) 1382
BspT104I TTCGAA 1 cut(s) 1352
BspT107I GGYRCC 3 cut(s) 107, 239, 1061
BspTNI GGTCTC 1 cut(s) 136
BsrBI CCGCTC 2 cut(s) 210, 506
BsrDI GCAATG 3 cut(s) 45, 1164, 1343
BsrFI RCCGGY 3 cut(s) 110, 236, 1058
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 3 cut(s) 110, 236, 1058
BssECI CCNNGG 5 cut(s) 513, 744, 831, 1135, 1264
BssMI GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
BssSI CACGAG 1 cut(s) 365
BssT1I CCWWGG 2 cut(s) 831, 1264
Bst2BI CACGAG 1 cut(s) 365
Bst2UI CCWGG 3 cut(s) 710, 746, 1137
Bst4CI ACNGT 5 cut(s) 121, 762, 1195, 1322, 1406
Bst6I CTCTTC 1 cut(s) 1382
BstBAI YACGTR 3 cut(s) 384, 441, 1304
BstBI TTCGAA 1 cut(s) 1352
BstC8I GCNNGC 4 cut(s) 436, 482, 496, 792
BstDEI CTNAG 5 cut(s) 75, 80, 695, 867, 1177
BstDSI CCRYGG 2 cut(s) 513, 1264
BstF5I GGATG 4 cut(s) 19, 201, 1316, 1420
BstFNI CGCG 3 cut(s) 446, 460, 658
BstH2I RGCGCY 1 cut(s) 742
BstHHI GCGC 3 cut(s) 658, 741, 1256
BstKTI GATC 7 cut(s) 19, 295, 357, 624, 883, 940, 1030
BstMAI GTCTC 3 cut(s) 136, 582, 1344
BstMBI GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
BstMCI CGRYCG 2 cut(s) 502, 513
BstMWI GCNNNNNNNGC 7 cut(s) 111, 481, 515, 529, 836, 860, 1389
BstNI CCWGG 3 cut(s) 710, 746, 1137
BstNSI RCATGY 1 cut(s) 498
BstSCI CCNGG 3 cut(s) 708, 744, 1135
BstUI CGCG 3 cut(s) 446, 460, 658
BstV1I GCAGC 7 cut(s) 519, 522, 774, 826, 841, 848, 883
BstX2I RGATCY 2 cut(s) 354, 1027
BstXI CCANNNNNNTGG 1 cut(s) 1271
BstYI RGATCY 2 cut(s) 354, 1027
BstZI CGGCCG 1 cut(s) 510
BsuRI GGCC 4 cut(s) 512, 518, 928, 1275
BtgI CCRYGG 2 cut(s) 513, 1264
BtrI CACGTC 5 cut(s) 424, 489, 670, 724, 1050
BtsCI GGATG 4 cut(s) 19, 201, 1316, 1420
BtsIMutI CAGTG 1 cut(s) 1185
Cac8I GCNNGC 4 cut(s) 436, 482, 496, 792
CaiI CAGNNNCTG 1 cut(s) 842
CciI TCATGA 1 cut(s) 984
CfoI GCGC 3 cut(s) 658, 741, 1256
Cfr10I RCCGGY 3 cut(s) 110, 236, 1058
Cfr13I GGNCC 2 cut(s) 1274, 1378
Csp6I GTAC 4 cut(s) 240, 442, 467, 679
CspAI ACCGGT 1 cut(s) 236
CviAII CATG 7 cut(s) 495, 985, 995, 1093, 1114, 1265, 1384
CviQI GTAC 4 cut(s) 240, 442, 467, 679
DdeI CTNAG 5 cut(s) 75, 80, 695, 867, 1177
DpnI GATC 7 cut(s) 18, 294, 356, 623, 882, 939, 1029
DpnII GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
EaeI YGGCCR 3 cut(s) 510, 516, 926
EagI CGGCCG 1 cut(s) 510
Eam1104I CTCTTC 1 cut(s) 1382
EarI CTCTTC 1 cut(s) 1382
EciI GGCGGA 1 cut(s) 467
Ecl136II GAGCTC 1 cut(s) 79
EclXI CGGCCG 1 cut(s) 510
Eco130I CCWWGG 2 cut(s) 831, 1264
Eco24I GRGCYC 3 cut(s) 51, 81, 770
Eco31I GGTCTC 1 cut(s) 136
Eco47I GGWCC 1 cut(s) 1378
Eco52I CGGCCG 1 cut(s) 510
Eco53kI GAGCTC 1 cut(s) 79
Eco57I CTGAAG 2 cut(s) 545, 1242
Eco72I CACGTG 1 cut(s) 384
Eco88I CYCGRG 2 cut(s) 139, 962
EcoICRI GAGCTC 1 cut(s) 79
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 3 cut(s) 708, 744, 1135
EcoT14I CCWWGG 2 cut(s) 831, 1264
EcoT38I GRGCYC 3 cut(s) 51, 81, 770
ErhI CCWWGG 2 cut(s) 831, 1264
Esp3I CGTCTC 2 cut(s) 582, 1344
FaeI CATG 7 cut(s) 498, 988, 998, 1096, 1117, 1268, 1387
FaqI GGGAC 1 cut(s) 85
FatI CATG 7 cut(s) 494, 984, 994, 1092, 1113, 1264, 1383
FauI CCCGC 3 cut(s) 62, 238, 531
FbaI TGATCA 1 cut(s) 16
FokI GGATG 4 cut(s) 26, 188, 1323, 1427
FriOI GRGCYC 3 cut(s) 51, 81, 770
FspBI CTAG 5 cut(s) 102, 125, 807, 1209, 1432
GlaI GCGC 3 cut(s) 657, 740, 1255
GsaI CCCAGC 1 cut(s) 677
GsuI CTGGAG 1 cut(s) 731
HaeII RGCGCY 1 cut(s) 742
HaeIII GGCC 4 cut(s) 512, 518, 928, 1275
HapII CCGG 4 cut(s) 44, 111, 237, 1059
HhaI GCGC 3 cut(s) 658, 741, 1256
Hin1II CATG 7 cut(s) 498, 988, 998, 1096, 1117, 1268, 1387
Hin6I GCGC 3 cut(s) 656, 739, 1254
HinP1I GCGC 3 cut(s) 656, 739, 1254
HincII GTYRAC 2 cut(s) 552, 1219
HindII GTYRAC 2 cut(s) 552, 1219
HinfI GANTC 4 cut(s) 8, 410, 455, 1205
HpaII CCGG 4 cut(s) 44, 111, 237, 1059
HphI GGTGA 3 cut(s) 263, 610, 763
Hpy166II GTNNAC 4 cut(s) 552, 667, 1219, 1402
Hpy188I TCNGA 5 cut(s) 7, 13, 354, 415, 733
Hpy188III TCNNGA 7 cut(s) 139, 141, 625, 807, 878, 935, 985
Hpy8I GTNNAC 4 cut(s) 552, 667, 1219, 1402
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 6 cut(s) 259, 520, 568, 628, 1004, 1348
HpyCH4III ACNGT 5 cut(s) 121, 762, 1195, 1322, 1406
HpyCH4IV ACGT 8 cut(s) 383, 423, 440, 488, 669, 723, 1049, 1303
HpyCH4V TGCA 6 cut(s) 90, 349, 484, 1119, 1348, 1366
HpyF10VI GCNNNNNNNGC 7 cut(s) 111, 481, 515, 529, 836, 860, 1389
HpyF3I CTNAG 5 cut(s) 75, 80, 695, 867, 1177
HpySE526I ACGT 8 cut(s) 383, 423, 440, 488, 669, 723, 1049, 1303
Hsp92II CATG 7 cut(s) 498, 988, 998, 1096, 1117, 1268, 1387
HspAI GCGC 3 cut(s) 656, 739, 1254
KpnI GGTACC 1 cut(s) 243
Ksp22I TGATCA 1 cut(s) 16
Kzo9I GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
LguI GCTCTTC 1 cut(s) 1382
LmnI GCTCC 6 cut(s) 46, 313, 480, 529, 746, 827
Lsp1109I GCAGC 7 cut(s) 519, 522, 774, 826, 841, 848, 883
LweI GCATC 3 cut(s) 358, 1031, 1301
MaeI CTAG 5 cut(s) 102, 125, 807, 1209, 1432
MaeII ACGT 8 cut(s) 383, 423, 440, 488, 669, 723, 1049, 1303
MaeIII GTNAC 1 cut(s) 907
MalI GATC 7 cut(s) 18, 294, 356, 623, 882, 939, 1029
MbiI CCGCTC 2 cut(s) 210, 506
MboI GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
MboII GAAGA 3 cut(s) 626, 785, 1399
MflI RGATCY 2 cut(s) 354, 1027
MhlI GDGCHC 6 cut(s) 51, 81, 366, 440, 477, 770
MluCI AATT 7 cut(s) 190, 578, 688, 755, 818, 930, 1197
MlyI GAGTC 3 cut(s) 17, 464, 1214
MmeI TCCRAC 2 cut(s) 387, 438
MseI TTAA 1 cut(s) 581
MslI CAYNNNNRTG 3 cut(s) 422, 1091, 1397
MspA1I CMGCKG 2 cut(s) 84, 839
MspI CCGG 4 cut(s) 44, 111, 237, 1059
MspR9I CCNGG 3 cut(s) 710, 746, 1137
Mva1269I GAATGC 2 cut(s) 1348, 1392
MvaI CCWGG 3 cut(s) 710, 746, 1137
MvnI CGCG 3 cut(s) 446, 460, 658
MwoI GCNNNNNNNGC 7 cut(s) 111, 481, 515, 529, 836, 860, 1389
NcoI CCATGG 1 cut(s) 1264
NdeII GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
NlaIII CATG 7 cut(s) 498, 988, 998, 1096, 1117, 1268, 1387
NlaIV GGNNCC 7 cut(s) 48, 109, 241, 309, 829, 1063, 1276
NspI RCATGY 1 cut(s) 498
NspV TTCGAA 1 cut(s) 1352
OliI CACNNNNGTG 1 cut(s) 422
PaeI GCATGC 1 cut(s) 498
PaeR7I CTCGAG 1 cut(s) 139
PagI TCATGA 1 cut(s) 984
PciSI GCTCTTC 1 cut(s) 1382
PcsI WCGNNNNNNNCGW 1 cut(s) 450
PctI GAATGC 2 cut(s) 1348, 1392
PfeI GAWTC 1 cut(s) 410
Pfl23II CGTACG 1 cut(s) 441
PinAI ACCGGT 1 cut(s) 236
PleI GAGTC 3 cut(s) 16, 463, 1213
PmaCI CACGTG 1 cut(s) 384
PmlI CACGTG 1 cut(s) 384
PpsI GAGTC 3 cut(s) 16, 463, 1213
Ppu21I YACGTR 3 cut(s) 384, 441, 1304
Psp124BI GAGCTC 1 cut(s) 81
Psp6I CCWGG 3 cut(s) 708, 744, 1135
PspCI CACGTG 1 cut(s) 384
PspFI CCCAGC 1 cut(s) 673
PspGI CCWGG 3 cut(s) 708, 744, 1135
PspLI CGTACG 1 cut(s) 441
PspN4I GGNNCC 7 cut(s) 48, 109, 241, 309, 829, 1063, 1276
PspPI GGNCC 2 cut(s) 1274, 1378
PstNI CAGNNNCTG 1 cut(s) 842
PsuI RGATCY 2 cut(s) 354, 1027
PvuII CAGCTG 2 cut(s) 84, 839
RsaI GTAC 4 cut(s) 241, 443, 468, 680
RsaNI GTAC 4 cut(s) 240, 442, 467, 679
RseI CAYNNNNRTG 3 cut(s) 422, 1091, 1397
SacI GAGCTC 1 cut(s) 81
SapI GCTCTTC 1 cut(s) 1382
SaqAI TTAA 1 cut(s) 581
Sau3AI GATC 7 cut(s) 16, 292, 354, 621, 880, 937, 1027
Sau96I GGNCC 2 cut(s) 1274, 1378
SchI GAGTC 3 cut(s) 17, 464, 1214
ScrFI CCNGG 3 cut(s) 710, 746, 1137
SduI GDGCHC 6 cut(s) 51, 81, 366, 440, 477, 770
SfaNI GCATC 3 cut(s) 358, 1031, 1301
Sfr274I CTCGAG 1 cut(s) 139
SfuI TTCGAA 1 cut(s) 1352
SinI GGWCC 1 cut(s) 1378
SlaI CTCGAG 1 cut(s) 139
SmiMI CAYNNNNRTG 3 cut(s) 422, 1091, 1397
SmlI CTYRAG 2 cut(s) 139, 359
SmoI CTYRAG 2 cut(s) 139, 359
SphI GCATGC 1 cut(s) 498
Sse9I AATT 7 cut(s) 190, 578, 688, 755, 818, 930, 1197
SspMI CTAG 5 cut(s) 102, 125, 807, 1209, 1432
SstI GAGCTC 1 cut(s) 81
StyD4I CCNGG 3 cut(s) 708, 744, 1135
StyI CCWWGG 2 cut(s) 831, 1264
TaaI ACNGT 5 cut(s) 121, 762, 1195, 1322, 1406
TaiI ACGT 8 cut(s) 386, 426, 443, 491, 672, 726, 1052, 1306
TaqI TCGA 9 cut(s) 140, 212, 225, 291, 396, 704, 918, 1293, 1352
TaqII GACCGA 1 cut(s) 516
TasI AATT 7 cut(s) 190, 578, 688, 755, 818, 930, 1197
TauI GCSGC 3 cut(s) 108, 509, 512
TfiI GAWTC 1 cut(s) 410
Tru1I TTAA 1 cut(s) 581
Tru9I TTAA 1 cut(s) 581
TscAI CASTG 1 cut(s) 1185
TseI GCWGC 7 cut(s) 532, 535, 787, 836, 839, 854, 871
TspDTI ATGAA 3 cut(s) 386, 1001, 1400
TspGWI ACGGA 4 cut(s) 434, 1020, 1103, 1326
TspRI CASTG 1 cut(s) 1185
VpaK11BI GGWCC 1 cut(s) 1378
XapI RAATTY 2 cut(s) 190, 688
XbaI TCTAGA 1 cut(s) 806
XceI RCATGY 1 cut(s) 498
XhoI CTCGAG 1 cut(s) 139
XspI CTAG 5 cut(s) 102, 125, 807, 1209, 1432
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.