pycom12g03660

deoxyribonuclease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009395
Physical Location & Seq
Forward (+)
3543054 .. 3545577
2524 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 516 bp
ATGTTTCTGCATATGCGTGCAGCTGCTCCAGATTTCTGTGAAATTGTAGAGAGAAATAGGGGCAGATTCAGCGCTGGGGTGGCCCACTCATTTACTGGTTGTGCAGAAGATCGTGATAAACTTCTTTCCATCAACAACATGTATATAGGAGTAAATGGTTGCTCTCTGAAGACAACTGAGAATCTCGACGTTGTGAGGGACATTCCTATTGAGAGGATGATGATTGAGACAGACTCTCCATATTGTGGAATCAAGAACACAAGTGCTGGGATTAAATTTGTGAAGTCCATTTGGCCTTCGAAGAAGAAAGACAAGTATGATCAAGAGTGCATTGTTAAAGACCGCAATGAACCGTGTTTGGTTCGGCAAGTTCTTGAGGTGGTAGCTGGTTGTAAAGGTATCAATGACATAGGTCAGCTGAGCCGGACATTGTATCACAACACTTGCAGGCTTTTCTTTCCTCAGGATTTGGATTCTGCAGCAGATTCTCTACTTGATGGTCATGACCCTCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
TatD_DNase

Protein Analysis

172

Amino Acids

19.2

Weight (kDa)

6.08

Isoelectric Point (pI)

32.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TatD_DNase PF01026 2 - 152 2.7e-20 TatD related DNase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 245
AciI CCGC 1 cut(s) 343
AcsI RAATTY 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 188
AfeI AGCGCT 1 cut(s) 73
AfiI CCNNNNNNNGG 1 cut(s) 245
AflIII ACRYGT 1 cut(s) 138
AluBI AGCT 3 cut(s) 23, 386, 418
AluI AGCT 3 cut(s) 23, 386, 418
Alw26I GTCTC 1 cut(s) 221
Aor51HI AGCGCT 1 cut(s) 73
AoxI GGCC 2 cut(s) 81, 293
ApeKI GCWGC 3 cut(s) 20, 23, 479
ApoI RAATTY 1 cut(s) 275
AspLEI GCGC 1 cut(s) 74
AspS9I GGNCC 1 cut(s) 82
AsuII TTCGAA 1 cut(s) 299
AxyI CCTNAGG 1 cut(s) 462
BbsI GAAGAC 1 cut(s) 176
BbvI GCAGC 3 cut(s) 10, 32, 491
BccI CCATC 2 cut(s) 137, 491
BclI TGATCA 1 cut(s) 319
BcoDI GTCTC 1 cut(s) 221
BfmI CTRYAG 1 cut(s) 477
BfoI RGCGCY 1 cut(s) 75
BisI GCNGC 3 cut(s) 21, 24, 480
BlpI GCTNAGC 1 cut(s) 419
BlsI GCNGC 3 cut(s) 22, 25, 481
BmgT120I GGNCC 1 cut(s) 82
BoxI GACNNNNGTC 1 cut(s) 411
BpiI GAAGAC 1 cut(s) 176
BplI GAGNNNNNCTC 2 cut(s) 218, 250
BpmI CTGGAG 1 cut(s) 12
Bpu1102I GCTNAGC 1 cut(s) 419
Bpu14I TTCGAA 1 cut(s) 299
BpuEI CTTGAG 1 cut(s) 395
BsaXI ACNNNNNCTCC 2 cut(s) 220, 250
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse1I ACTGG 1 cut(s) 100
Bse21I CCTNAGG 1 cut(s) 462
Bse3DI GCAATG 1 cut(s) 352
BseGI GGATG 1 cut(s) 222
BseLI CCNNNNNNNGG 1 cut(s) 245
BseMI GCAATG 1 cut(s) 352
BseMII CTCAG 3 cut(s) 168, 410, 476
BseNI ACTGG 1 cut(s) 100
BseXI GCAGC 3 cut(s) 10, 32, 491
BseYI CCCAGC 2 cut(s) 74, 266
BsgI GTGCAG 2 cut(s) 39, 123
BshFI GGCC 2 cut(s) 83, 295
BsiSI CCGG 1 cut(s) 424
BslFI GGGAC 1 cut(s) 212
BslI CCNNNNNNNGG 1 cut(s) 245
BsmAI GTCTC 1 cut(s) 221
BsmFI GGGAC 1 cut(s) 212
BsnI GGCC 2 cut(s) 83, 295
Bsp119I TTCGAA 1 cut(s) 299
Bsp143I GATC 2 cut(s) 109, 319
Bsp1720I GCTNAGC 1 cut(s) 419
BspACI CCGC 1 cut(s) 343
BspANI GGCC 2 cut(s) 83, 295
BspCNI CTCAG 3 cut(s) 169, 411, 475
BspHI TCATGA 1 cut(s) 502
BspMAI CTGCAG 1 cut(s) 481
BspT104I TTCGAA 1 cut(s) 299
BsrDI GCAATG 1 cut(s) 352
BsrI ACTGG 1 cut(s) 100
BssMI GATC 2 cut(s) 109, 319
Bst4CI ACNGT 1 cut(s) 354
BstBI TTCGAA 1 cut(s) 299
BstC8I GCNNGC 2 cut(s) 18, 449
BstDEI CTNAG 4 cut(s) 177, 419, 462, 510
BstF5I GGATG 1 cut(s) 222
BstH2I RGCGCY 1 cut(s) 75
BstHHI GCGC 1 cut(s) 74
BstKTI GATC 2 cut(s) 112, 322
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 109, 319
BstMWI GCNNNNNNNGC 2 cut(s) 69, 80
BstNSI RCATGY 1 cut(s) 142
BstPAI GACNNNNGTC 1 cut(s) 411
BstSFI CTRYAG 1 cut(s) 477
BstV1I GCAGC 3 cut(s) 10, 32, 491
BstV2I GAAGAC 1 cut(s) 176
Bsu36I CCTNAGG 1 cut(s) 462
BsuRI GGCC 2 cut(s) 83, 295
BtsCI GGATG 1 cut(s) 222
Cac8I GCNNGC 2 cut(s) 18, 449
CciI TCATGA 1 cut(s) 502
CfoI GCGC 1 cut(s) 74
Cfr13I GGNCC 1 cut(s) 82
CviAII CATG 2 cut(s) 139, 503
CviJI RGCY 7 cut(s) 23, 83, 295, 386, 418, 423, 451
CviKI_1 RGCY 7 cut(s) 23, 83, 295, 386, 418, 423, 451
DdeI CTNAG 4 cut(s) 177, 419, 462, 510
DpnI GATC 2 cut(s) 111, 321
DpnII GATC 2 cut(s) 109, 319
Eco47III AGCGCT 1 cut(s) 73
Eco57I CTGAAG 1 cut(s) 188
Eco81I CCTNAGG 1 cut(s) 462
FaeI CATG 2 cut(s) 142, 506
FaiI YATR 9 cut(s) 12, 14, 140, 144, 146, 241, 318, 410, 504
FaqI GGGAC 1 cut(s) 212
FatI CATG 2 cut(s) 138, 502
FauNDI CATATG 1 cut(s) 12
FbaI TGATCA 1 cut(s) 319
Fnu4HI GCNGC 3 cut(s) 21, 24, 480
FokI GGATG 1 cut(s) 229
Fsp4HI GCNGC 3 cut(s) 21, 24, 480
GlaI GCGC 1 cut(s) 73
GluI GCNGC 3 cut(s) 21, 24, 480
GsaI CCCAGC 2 cut(s) 78, 270
GsuI CTGGAG 1 cut(s) 12
HaeII RGCGCY 1 cut(s) 75
HaeIII GGCC 2 cut(s) 83, 295
HapII CCGG 1 cut(s) 424
HhaI GCGC 1 cut(s) 74
Hin1II CATG 2 cut(s) 142, 506
Hin6I GCGC 1 cut(s) 72
HinP1I GCGC 1 cut(s) 72
HinfI GANTC 6 cut(s) 66, 181, 233, 249, 473, 485
HpaII CCGG 1 cut(s) 424
Hpy188I TCNGA 1 cut(s) 168
Hpy188III TCNNGA 8 cut(s) 29, 113, 185, 253, 323, 374, 464, 503
Hpy99I CGWCG 1 cut(s) 191
HpyAV CCTTC 1 cut(s) 306
HpyCH4III ACNGT 1 cut(s) 354
HpyCH4IV ACGT 1 cut(s) 189
HpyCH4V TGCA 6 cut(s) 10, 20, 104, 330, 447, 479
HpyF10VI GCNNNNNNNGC 2 cut(s) 69, 80
HpyF3I CTNAG 4 cut(s) 177, 419, 462, 510
HpySE526I ACGT 1 cut(s) 189
Hsp92II CATG 2 cut(s) 142, 506
HspAI GCGC 1 cut(s) 72
Ksp22I TGATCA 1 cut(s) 319
Kzo9I GATC 2 cut(s) 109, 319
LmnI GCTCC 1 cut(s) 31
LpnPI CCDG 8 cut(s) 42, 60, 81, 252, 372, 433, 437, 449
Lsp1109I GCAGC 3 cut(s) 10, 32, 491
MaeII ACGT 1 cut(s) 189
MalI GATC 2 cut(s) 111, 321
MboI GATC 2 cut(s) 109, 319
MboII GAAGA 4 cut(s) 119, 181, 313, 316
MluCI AATT 2 cut(s) 42, 275
MlyI GAGTC 1 cut(s) 227
MnlI CCTC 4 cut(s) 189, 207, 370, 471
MseI TTAA 2 cut(s) 273, 336
MslI CAYNNNNRTG 1 cut(s) 15
MspA1I CMGCKG 2 cut(s) 23, 418
MspI CCGG 1 cut(s) 424
MwoI GCNNNNNNNGC 2 cut(s) 69, 80
NdeI CATATG 1 cut(s) 12
NdeII GATC 2 cut(s) 109, 319
NlaIII CATG 2 cut(s) 142, 506
NspI RCATGY 1 cut(s) 142
NspV TTCGAA 1 cut(s) 299
PagI TCATGA 1 cut(s) 502
PciI ACATGT 1 cut(s) 138
PfeI GAWTC 5 cut(s) 66, 181, 249, 473, 485
PflMI CCANNNNNTGG 1 cut(s) 245
PkrI GCNGC 3 cut(s) 22, 25, 481
PleI GAGTC 1 cut(s) 227
PpsI GAGTC 1 cut(s) 227
PscI ACATGT 1 cut(s) 138
PshAI GACNNNNGTC 1 cut(s) 411
PspFI CCCAGC 2 cut(s) 74, 266
PspPI GGNCC 1 cut(s) 82
PstI CTGCAG 1 cut(s) 481
PvuII CAGCTG 2 cut(s) 23, 418
RseI CAYNNNNRTG 1 cut(s) 15
SaqAI TTAA 2 cut(s) 273, 336
SatI GCNGC 3 cut(s) 21, 24, 480
Sau3AI GATC 2 cut(s) 109, 319
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 1 cut(s) 227
SetI ASST 7 cut(s) 25, 192, 381, 388, 400, 415, 420
SfcI CTRYAG 1 cut(s) 477
SfuI TTCGAA 1 cut(s) 299
SmiMI CAYNNNNRTG 1 cut(s) 15
SmlI CTYRAG 1 cut(s) 374
SmoI CTYRAG 1 cut(s) 374
Sse9I AATT 2 cut(s) 42, 275
SsiI CCGC 1 cut(s) 343
TaaI ACNGT 1 cut(s) 354
TaiI ACGT 1 cut(s) 192
TaqI TCGA 2 cut(s) 186, 299
TasI AATT 2 cut(s) 42, 275
TfiI GAWTC 5 cut(s) 66, 181, 249, 473, 485
Tru1I TTAA 2 cut(s) 273, 336
Tru9I TTAA 2 cut(s) 273, 336
TseI GCWGC 3 cut(s) 20, 23, 479
TspDTI ATGAA 1 cut(s) 363
Van91I CCANNNNNTGG 1 cut(s) 245
XapI RAATTY 1 cut(s) 275
XceI RCATGY 1 cut(s) 142
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.