pycom12g04410

BTB POZ domain-containing protein At3g05675-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0003442
Physical Location & Seq
Forward (+)
4261780 .. 4263276
1497 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1380 bp
ATGCTTATGTACAGATTCCAACATGTGTTCAAGGTATACTCATTAGTGCTTAACGCTCCAATGGACTATCTTGGTTCTGAAAAAAGTACGTCTAGCCGGATTGGGGACCGATCAACCAGTGATGTTGTTGTGAGGATACGCACAGAGGATGGGCGGGATGATCGGCTATATTGTCATTCCCAGATCCTCATGGAAAAGAGCAAGTATTTCGCTGATCGCCTATCTGAAAATTGGCCAACATGTCAGATTCTTGACTCACGCAACTGCGTTGACGTATACTGCCAAGAATCAGAATTTGACCATCATGTCATTGTTCTCCGCCTTTTTTACGTCGCAGTAGATGGCTTAGTTGGTAACTTGTGGCATGGTGTAAAAAACGCCATTGGAATTCTCCAGGTGGCTGTCAAGCTCGGATGCCCACAAATCATAACTGCTTGTGTGAACTACTTAGAAGCAGTTTCCTGGGAAGAGTCCGAGGAGGAGGACATTTTAAAGATCGTACCACGCCTGGGATCACAAGTGGAGCCAATTCTTGCCCGTCTTCTTCCAGTCAATCACTCATCAATTATGGGGATATTTTTGTCAACCATCCGATTTGCCACATCGTGCCCTCCTCCAATCATGAATGATCTCAAGTCTTCTGCCCAGGAGCAGCTTGAGTACATGATTACTGAAGATGATGATGCCCCGCTCTTAACAGCCGATGATGAGATCAAATCCCAGGTGAAAGAATGTGTGAATAGACTATTTGGAAGATTTAGTAATCTATTAGAAGCCTTGTTATGTGAAGTAGAATTATCTTCTGATGGAGGGAAGATGCAGTCGTTTCAAACGTTTTTATCAGATCTGTCGTGGACATGCCAGATATTAACTAAGTTGGAAACTATGAGGGAACTTGTCTGCAATTGGATGGATGCATCGGATAAAATAGTTAACGTTGTAAAGCAAGCAAGGCCGACAGCTGAACTAATTGAGACAAAGCTTAGGATTGTTGAGGTTGCAGCAAAGATTCTAGAGGCAATAGGTTACGGCACTGTCATTTTGCCAACTGCAAAACGGCTTCATATGGTTAAGGTGTGGCTTCCTTTTGTGAGGGTTGCAAAACCGGTGATCGATTCTGCCACAACTACCGGTGATGACGCTCCTGCGCTAAAGATGGATAGTGAACTGTGGCAATCCTTGGAATCCACATTTGTTTCCATAATTCTTACGCTTCCATCCGTCGAGCAGGCGGAGATATTGACTGAATGGTTGGAGAATGAACATATTCAATATCCTGACCTGACCGAGGCATTTGAGGTATGGTGTTACAGATCCAAGGTTTCCAAAAGAAGACTATCATTTATATCGGTGAACCATGAGAAAATCGATACACATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB

Protein Analysis

460

Amino Acids

52.15

Weight (kDa)

5.05

Isoelectric Point (pI)

47.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BTB-POZ_ANK-like PF25553 236 - 440 3.2e-06 BTB/POZ Ankyrin-like repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 305
AccBSI CCGCTC 1 cut(s) 691
AccI GTMKAC 2 cut(s) 36, 276
AciI CCGC 4 cut(s) 154, 319, 689, 1232
AclI AACGTT 2 cut(s) 833, 936
AclWI GGATC 3 cut(s) 178, 520, 1308
AcoI YGGCCR 1 cut(s) 233
AcsI RAATTY 2 cut(s) 293, 387
AcuI CTGAAG 1 cut(s) 693
AdeI CACNNNGTG 1 cut(s) 606
AfaI GTAC 4 cut(s) 11, 88, 501, 662
AfiI CCNNNNNNNGG 3 cut(s) 103, 509, 1288
AflIII ACRYGT 2 cut(s) 22, 239
AgeI ACCGGT 2 cut(s) 1105, 1130
AgsI TTSAA 3 cut(s) 31, 830, 1271
AjnI CCWGG 5 cut(s) 393, 461, 507, 645, 720
AluBI AGCT 4 cut(s) 409, 655, 962, 982
AluI AGCT 4 cut(s) 409, 655, 962, 982
Alw26I GTCTC 1 cut(s) 968
AlwI GGATC 3 cut(s) 178, 520, 1308
AoxI GGCC 2 cut(s) 233, 953
ApeKI GCWGC 2 cut(s) 652, 1001
ApoI RAATTY 2 cut(s) 293, 387
AsiGI ACCGGT 2 cut(s) 1105, 1130
Asp700I GAANNNNTTC 1 cut(s) 1266
AspLEI GCGC 1 cut(s) 1150
AspS9I GGNCC 1 cut(s) 106
AsuHPI GGTGA 4 cut(s) 736, 1120, 1145, 1363
AvaII GGWCC 1 cut(s) 106
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 235
BbsI GAAGAC 3 cut(s) 533, 630, 1339
BbvI GCAGC 2 cut(s) 664, 1013
BccI CCATC 8 cut(s) 143, 309, 335, 596, 800, 904, 1150, 1225
BceAI ACGGC 2 cut(s) 1045, 1073
BcgI CGANNNNNNTGC 2 cut(s) 190, 224
BciT130I CCWGG 5 cut(s) 395, 463, 509, 647, 722
BciVI GTATCC 1 cut(s) 129
BcoDI GTCTC 1 cut(s) 968
BfaI CTAG 2 cut(s) 93, 1013
BfuI GTATCC 1 cut(s) 129
BglII AGATCT 1 cut(s) 844
BisI GCNGC 2 cut(s) 653, 1002
BlsI GCNGC 2 cut(s) 654, 1003
Bme1390I CCNGG 5 cut(s) 395, 463, 509, 647, 722
Bme18I GGWCC 1 cut(s) 106
BmgT120I GGNCC 1 cut(s) 106
BmiI GGNNCC 2 cut(s) 107, 525
BmrFI CCNGG 5 cut(s) 395, 463, 509, 647, 722
BmsI GCATC 5 cut(s) 404, 673, 807, 904, 926
BpiI GAAGAC 3 cut(s) 533, 630, 1339
BpmI CTGGAG 1 cut(s) 377
Bpu10I CCTNAGC 1 cut(s) 983
BpuEI CTTGAG 2 cut(s) 617, 677
Bsa29I ATCGAT 2 cut(s) 1113, 1368
BsaJI CCNNGG 8 cut(s) 462, 474, 508, 645, 720, 1179, 1287, 1317
BsaWI WCCGGW 2 cut(s) 1105, 1130
Bsc4I CCNNNNNNNGG 3 cut(s) 103, 509, 1288
Bse118I RCCGGY 2 cut(s) 1105, 1130
Bse1I ACTGG 2 cut(s) 117, 548
BseBI CCWGG 5 cut(s) 395, 463, 509, 647, 722
BseCI ATCGAT 2 cut(s) 1113, 1368
BseDI CCNNGG 8 cut(s) 462, 474, 508, 645, 720, 1179, 1287, 1317
BseGI GGATG 7 cut(s) 154, 163, 419, 588, 915, 919, 1217
BseLI CCNNNNNNNGG 3 cut(s) 103, 509, 1288
BseNI ACTGG 2 cut(s) 117, 548
BseRI GAGGAG 3 cut(s) 491, 494, 603
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 2 cut(s) 664, 1013
BshFI GGCC 2 cut(s) 235, 955
BshTI ACCGGT 2 cut(s) 1105, 1130
BshVI ATCGAT 2 cut(s) 1113, 1368
BsiSI CCGG 3 cut(s) 97, 1106, 1131
BslFI GGGAC 1 cut(s) 119
BslI CCNNNNNNNGG 3 cut(s) 103, 509, 1288
BsmAI GTCTC 1 cut(s) 968
BsmFI GGGAC 1 cut(s) 119
BsnI GGCC 2 cut(s) 235, 955
Bsp1286I GDGCHC 1 cut(s) 611
Bsp1407I TGTACA 1 cut(s) 9
BspACI CCGC 4 cut(s) 154, 319, 689, 1232
BspANI GGCC 2 cut(s) 235, 955
BspDI ATCGAT 2 cut(s) 1113, 1368
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 2 cut(s) 107, 525
BspPI GGATC 3 cut(s) 178, 520, 1308
BsrBI CCGCTC 1 cut(s) 691
BsrFI RCCGGY 2 cut(s) 1105, 1130
BsrGI TGTACA 1 cut(s) 9
BsrI ACTGG 2 cut(s) 117, 548
BssAI RCCGGY 2 cut(s) 1105, 1130
BssECI CCNNGG 8 cut(s) 462, 474, 508, 645, 720, 1179, 1287, 1317
BssNAI GTATAC 2 cut(s) 37, 277
BssT1I CCWWGG 2 cut(s) 1179, 1317
Bst1107I GTATAC 2 cut(s) 37, 277
Bst2UI CCWGG 5 cut(s) 395, 463, 509, 647, 722
Bst4CI ACNGT 2 cut(s) 1036, 1170
Bst6I CTCTTC 1 cut(s) 462
BstAUI TGTACA 1 cut(s) 9
BstC8I GCNNGC 2 cut(s) 948, 1230
BstDEI CTNAG 4 cut(s) 346, 448, 873, 983
BstENI CCTNNNNNAGG 1 cut(s) 1286
BstF5I GGATG 7 cut(s) 154, 163, 419, 588, 915, 919, 1217
BstHHI GCGC 1 cut(s) 1150
BstMAI GTCTC 1 cut(s) 968
BstMWI GCNNNNNNNGC 1 cut(s) 952
BstNI CCWGG 5 cut(s) 395, 463, 509, 647, 722
BstNSI RCATGY 3 cut(s) 26, 243, 861
BstSCI CCNGG 5 cut(s) 393, 461, 507, 645, 720
BstSLI GKGCMC 1 cut(s) 611
BstV1I GCAGC 2 cut(s) 664, 1013
BstV2I GAAGAC 3 cut(s) 533, 630, 1339
BstX2I RGATCY 3 cut(s) 183, 844, 1313
BstYI RGATCY 3 cut(s) 183, 844, 1313
BstZ17I GTATAC 2 cut(s) 37, 277
Bsu15I ATCGAT 2 cut(s) 1113, 1368
BsuI GTATCC 1 cut(s) 129
BsuRI GGCC 2 cut(s) 235, 955
BsuTUI ATCGAT 2 cut(s) 1113, 1368
BtsCI GGATG 7 cut(s) 154, 163, 419, 588, 915, 919, 1217
BtsIMutI CAGTG 2 cut(s) 124, 1032
Cac8I GCNNGC 2 cut(s) 948, 1230
CciI TCATGA 1 cut(s) 621
CfoI GCGC 1 cut(s) 1150
Cfr10I RCCGGY 2 cut(s) 1105, 1130
Cfr13I GGNCC 1 cut(s) 106
ClaI ATCGAT 2 cut(s) 1113, 1368
CseI GACGC 1 cut(s) 1148
Csp6I GTAC 4 cut(s) 10, 87, 500, 661
CspAI ACCGGT 2 cut(s) 1105, 1130
CviAII CATG 9 cut(s) 23, 190, 240, 305, 365, 622, 664, 858, 1358
CviQI GTAC 4 cut(s) 10, 87, 500, 661
DdeI CTNAG 4 cut(s) 346, 448, 873, 983
DraI TTTAAA 1 cut(s) 492
DraIII CACNNNGTG 1 cut(s) 606
DrdI GACNNNNNNGTC 1 cut(s) 305
DseDI GACNNNNNNGTC 1 cut(s) 305
EaeI YGGCCR 1 cut(s) 233
Eam1104I CTCTTC 1 cut(s) 462
EarI CTCTTC 1 cut(s) 462
EciI GGCGGA 2 cut(s) 308, 1247
Eco130I CCWWGG 2 cut(s) 1179, 1317
Eco47I GGWCC 1 cut(s) 106
Eco57I CTGAAG 1 cut(s) 693
EcoNI CCTNNNNNAGG 1 cut(s) 1286
EcoRI GAATTC 1 cut(s) 387
EcoRII CCWGG 5 cut(s) 393, 461, 507, 645, 720
EcoT14I CCWWGG 2 cut(s) 1179, 1317
EcoT22I ATGCAT 1 cut(s) 919
ErhI CCWWGG 2 cut(s) 1179, 1317
FaeI CATG 9 cut(s) 26, 193, 243, 308, 368, 625, 667, 861, 1361
FaqI GGGAC 1 cut(s) 119
FatI CATG 9 cut(s) 22, 189, 239, 304, 364, 621, 663, 857, 1357
FauI CCCGC 2 cut(s) 147, 696
FauNDI CATATG 1 cut(s) 1065
FblI GTMKAC 2 cut(s) 36, 276
Fnu4HI GCNGC 2 cut(s) 653, 1002
FokI GGATG 7 cut(s) 161, 170, 426, 575, 922, 926, 1204
Fsp4HI GCNGC 2 cut(s) 653, 1002
FspBI CTAG 2 cut(s) 93, 1013
GlaI GCGC 1 cut(s) 1149
GluI GCNGC 2 cut(s) 653, 1002
GsuI CTGGAG 1 cut(s) 377
HaeIII GGCC 2 cut(s) 235, 955
HapII CCGG 3 cut(s) 97, 1106, 1131
HgaI GACGC 1 cut(s) 1148
HhaI GCGC 1 cut(s) 1150
Hin1II CATG 9 cut(s) 26, 193, 243, 308, 368, 625, 667, 861, 1361
Hin6I GCGC 1 cut(s) 1148
HinP1I GCGC 1 cut(s) 1148
HincII GTYRAC 3 cut(s) 271, 585, 934
HindII GTYRAC 3 cut(s) 271, 585, 934
HindIII AAGCTT 1 cut(s) 980
HinfI GANTC 8 cut(s) 15, 247, 254, 287, 470, 1009, 1115, 1184
HpaI GTTAAC 1 cut(s) 934
HpaII CCGG 3 cut(s) 97, 1106, 1131
HphI GGTGA 4 cut(s) 736, 1120, 1145, 1363
Hpy166II GTNNAC 9 cut(s) 37, 271, 277, 442, 585, 855, 934, 1166, 1354
Hpy188III TCNNGA 4 cut(s) 251, 622, 1013, 1277
Hpy8I GTNNAC 9 cut(s) 37, 271, 277, 442, 585, 855, 934, 1166, 1354
Hpy99I CGWCG 2 cut(s) 335, 1226
HpyCH4III ACNGT 2 cut(s) 1036, 1170
HpyCH4IV ACGT 5 cut(s) 89, 273, 330, 833, 936
HpyCH4V TGCA 6 cut(s) 820, 903, 917, 1001, 1052, 1100
HpyF10VI GCNNNNNNNGC 1 cut(s) 952
HpyF3I CTNAG 4 cut(s) 346, 448, 873, 983
HpySE526I ACGT 5 cut(s) 89, 273, 330, 833, 936
Hsp92II CATG 9 cut(s) 26, 193, 243, 308, 368, 625, 667, 861, 1361
HspAI GCGC 1 cut(s) 1148
KspAI GTTAAC 1 cut(s) 934
LmnI GCTCC 4 cut(s) 61, 523, 649, 1147
Lsp1109I GCAGC 2 cut(s) 664, 1013
LweI GCATC 5 cut(s) 404, 673, 807, 904, 926
MaeI CTAG 2 cut(s) 93, 1013
MaeII ACGT 5 cut(s) 89, 273, 330, 833, 936
MaeIII GTNAC 3 cut(s) 353, 1025, 1307
MbiI CCGCTC 1 cut(s) 691
MboII GAAGA 9 cut(s) 479, 533, 536, 630, 686, 765, 792, 826, 1344
MfeI CAATTG 1 cut(s) 904
MflI RGATCY 3 cut(s) 183, 844, 1313
MhlI GDGCHC 1 cut(s) 611
MlsI TGGCCA 1 cut(s) 235
MluCI AATT 9 cut(s) 229, 293, 387, 528, 564, 794, 904, 969, 1203
MluNI TGGCCA 1 cut(s) 235
MlyI GAGTC 2 cut(s) 248, 479
MmeI TCCRAC 3 cut(s) 43, 858, 1233
Mox20I TGGCCA 1 cut(s) 235
Mph1103I ATGCAT 1 cut(s) 919
MroXI GAANNNNTTC 1 cut(s) 1266
MscI TGGCCA 1 cut(s) 235
MseI TTAA 7 cut(s) 51, 491, 695, 869, 933, 1071, 1378
Msp20I TGGCCA 1 cut(s) 235
MspA1I CMGCKG 1 cut(s) 962
MspI CCGG 3 cut(s) 97, 1106, 1131
MspR9I CCNGG 5 cut(s) 395, 463, 509, 647, 722
MunI CAATTG 1 cut(s) 904
MvaI CCWGG 5 cut(s) 395, 463, 509, 647, 722
MwoI GCNNNNNNNGC 1 cut(s) 952
NdeI CATATG 1 cut(s) 1065
NlaIII CATG 9 cut(s) 26, 193, 243, 308, 368, 625, 667, 861, 1361
NlaIV GGNNCC 2 cut(s) 107, 525
NsiI ATGCAT 1 cut(s) 919
NspI RCATGY 3 cut(s) 26, 243, 861
PagI TCATGA 1 cut(s) 621
PciI ACATGT 2 cut(s) 22, 239
PcsI WCGNNNNNNNCGW 1 cut(s) 830
PdmI GAANNNNTTC 1 cut(s) 1266
PfeI GAWTC 6 cut(s) 15, 247, 287, 1009, 1115, 1184
PinAI ACCGGT 2 cut(s) 1105, 1130
PkrI GCNGC 2 cut(s) 654, 1003
PleI GAGTC 2 cut(s) 248, 478
PpsI GAGTC 2 cut(s) 248, 478
PscI ACATGT 2 cut(s) 22, 239
Psp1406I AACGTT 2 cut(s) 833, 936
Psp6I CCWGG 5 cut(s) 393, 461, 507, 645, 720
PspGI CCWGG 5 cut(s) 393, 461, 507, 645, 720
PspN4I GGNNCC 2 cut(s) 107, 525
PspPI GGNCC 1 cut(s) 106
PsuI RGATCY 3 cut(s) 183, 844, 1313
PvuII CAGCTG 1 cut(s) 962
RsaI GTAC 4 cut(s) 11, 88, 501, 662
RsaNI GTAC 4 cut(s) 10, 87, 500, 661
SaqAI TTAA 7 cut(s) 51, 491, 695, 869, 933, 1071, 1378
SatI GCNGC 2 cut(s) 653, 1002
Sau96I GGNCC 1 cut(s) 106
SchI GAGTC 2 cut(s) 248, 479
ScrFI CCNGG 5 cut(s) 395, 463, 509, 647, 722
SduI GDGCHC 1 cut(s) 611
SfaNI GCATC 5 cut(s) 404, 673, 807, 904, 926
SinI GGWCC 1 cut(s) 106
SmlI CTYRAG 2 cut(s) 632, 656
SmoI CTYRAG 2 cut(s) 632, 656
Sse9I AATT 9 cut(s) 229, 293, 387, 528, 564, 794, 904, 969, 1203
SsiI CCGC 4 cut(s) 154, 319, 689, 1232
SspMI CTAG 2 cut(s) 93, 1013
StyD4I CCNGG 5 cut(s) 393, 461, 507, 645, 720
StyI CCWWGG 2 cut(s) 1179, 1317
TaaI ACNGT 2 cut(s) 1036, 1170
TaiI ACGT 5 cut(s) 92, 276, 333, 836, 939
TaqI TCGA 3 cut(s) 1113, 1224, 1368
TaqII GACCGA 2 cut(s) 123, 1301
TasI AATT 9 cut(s) 229, 293, 387, 528, 564, 794, 904, 969, 1203
TatI WGTACW 2 cut(s) 9, 660
TfiI GAWTC 6 cut(s) 15, 247, 287, 1009, 1115, 1184
Tru1I TTAA 7 cut(s) 51, 491, 695, 869, 933, 1071, 1378
Tru9I TTAA 7 cut(s) 51, 491, 695, 869, 933, 1071, 1378
TscAI CASTG 2 cut(s) 124, 1039
TseI GCWGC 2 cut(s) 652, 1001
TspDTI ATGAA 3 cut(s) 638, 1052, 1275
TspGWI ACGGA 1 cut(s) 1210
TspRI CASTG 2 cut(s) 124, 1039
VpaK11BI GGWCC 1 cut(s) 106
XagI CCTNNNNNAGG 1 cut(s) 1286
XapI RAATTY 2 cut(s) 293, 387
XbaI TCTAGA 1 cut(s) 1012
XceI RCATGY 3 cut(s) 26, 243, 861
XmiI GTMKAC 2 cut(s) 36, 276
XmnI GAANNNNTTC 1 cut(s) 1266
XspI CTAG 2 cut(s) 93, 1013
Zsp2I ATGCAT 1 cut(s) 919
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.