pycom12g07360

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0016934
Physical Location & Seq
Forward (+)
7682083 .. 7682694
612 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g07360.1

Sequence Viewer

Length: 612 bp
ATGATGAACTTTTACCGCCAAGGACATGAAGGGAAGGGTGTAGATATTGGTGAAGCGACCGACATTCTTCAGGAAATGGAGAAGCGTGGGAAACACCCAACTGTTGTGATATACGCCAAATATATTCATGCACTTTGTAAAGTTGGATGGGTTGAGTTTGTTTTGGACTTTATTCGAAACTTGAGATGCAGAAATCAACCTCGTAATAGTTACTGTTATAACGCCATACTTCTTGGATTTTGCCAAAAAGGTGAAACGTATGAAGCTTGGAAGATTTTGGAAGAAATGAAGACGTATGGGACAATACCAGATGTTTATTGCTACACCATTTTAATAGGTGGATTTTGCAAGGAGGGAGATATTGAGATTGCTCTCACTTTATTTGAAGAAATGGAGCATTATAAAATTGAACCGTCTTTAGTTAGCTATAGCTCACTGATATACGGCCTTTGTAAGATTGGGTTGGTGGATGACTCACTGGATATTTTCCTTGATCTTGAAGCTGCTGGGGTGTGCCCTGATGCCATCACTTGTACTCTCATTGTTGAAGGGTACTGTAGGGAAGGACTGTTGAAGGGTACTGTAGGGAAGGACGCTTGGAGGAAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

204

Amino Acids

23.08

Weight (kDa)

5.19

Isoelectric Point (pI)

27.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_1 PF12854 68 - 97 4.6e-06 PPR repeat
PPR_2 PF13041 69 - 117 2.2e-16 PPR repeat family
PPR_long PF17177 85 - 174 7.4e-08 Pentacotripeptide-repeat region of PRORP
PPR_3 PF13812 91 - 147 2.2e-11 Pentatricopeptide repeat domain
PPR_2 PF13041 103 - 152 1.6e-16 PPR repeat family
PPR_1 PF12854 103 - 132 1.4e-11 PPR repeat
PPR PF01535 107 - 133 1.8e-07 PPR repeat
PPR_1 PF12854 136 - 163 2.8e-06 PPR repeat
PPR_2 PF13041 138 - 187 1.2e-08 PPR repeat family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016934)

Species Orthologous Gene IDs
malus_domestica MD14G1081200.v1.1
pyrus_communis pycom12g07360 pycom14g07050
rosa_chinensis RchiOBHm_Chr3g0484571
rosa_laevigata RLG00000023219
rosa_multiflora Rmu_co8455711.1_g000001 Rmu_sc0000295.1_g000038 Rmu_sc0032195.1_g000001
rosa_roxburghii Rroxscaffold_6G00397630
rosa_rugosa Rorug03G0211700
rosa_samantha Rh3AG262500 Rh3BG297800 Rh3CG294800 Rh3DG291100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 219, 402
AciI CCGC 1 cut(s) 16
AcuI CTGAAG 1 cut(s) 53
AfaI GTAC 3 cut(s) 535, 554, 580
AgsI TTSAA 5 cut(s) 386, 410, 500, 548, 574
AluBI AGCT 4 cut(s) 266, 426, 432, 503
AluI AGCT 4 cut(s) 266, 426, 432, 503
AoxI GGCC 1 cut(s) 445
ApeKI GCWGC 1 cut(s) 503
AsuHPI GGTGA 2 cut(s) 62, 263
AsuII TTCGAA 1 cut(s) 175
BaeGI GKGCMC 1 cut(s) 518
BbsI GAAGAC 1 cut(s) 296
BbvI GCAGC 1 cut(s) 490
BccI CCATC 2 cut(s) 141, 533
BceAI ACGGC 1 cut(s) 460
BfmI CTRYAG 3 cut(s) 427, 556, 582
BisI GCNGC 1 cut(s) 504
BlsI GCNGC 1 cut(s) 505
BmsI GCATC 2 cut(s) 176, 511
BpiI GAAGAC 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 175
BpuEI CTTGAG 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 19
Bse1I ACTGG 1 cut(s) 483
BseDI CCNNGG 1 cut(s) 19
BseGI GGATG 2 cut(s) 152, 475
BseNI ACTGG 1 cut(s) 483
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 1 cut(s) 490
BseYI CCCAGC 1 cut(s) 506
Bsh1285I CGRYCG 1 cut(s) 60
BshFI GGCC 1 cut(s) 447
BsiEI CGRYCG 1 cut(s) 60
BslFI GGGAC 1 cut(s) 313
BsmFI GGGAC 1 cut(s) 313
BsnI GGCC 1 cut(s) 447
Bsp119I TTCGAA 1 cut(s) 175
Bsp1286I GDGCHC 1 cut(s) 518
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 1 cut(s) 16
BspANI GGCC 1 cut(s) 447
BspT104I TTCGAA 1 cut(s) 175
BsrI ACTGG 1 cut(s) 483
BssECI CCNNGG 1 cut(s) 19
BssMI GATC 1 cut(s) 493
BssT1I CCWWGG 1 cut(s) 19
Bst4CI ACNGT 6 cut(s) 103, 215, 414, 557, 570, 583
BstBI TTCGAA 1 cut(s) 175
BstF5I GGATG 2 cut(s) 152, 475
BstKTI GATC 1 cut(s) 496
BstMBI GATC 1 cut(s) 493
BstMCI CGRYCG 1 cut(s) 60
BstSFI CTRYAG 3 cut(s) 427, 556, 582
BstSLI GKGCMC 1 cut(s) 518
BstV1I GCAGC 1 cut(s) 490
BstV2I GAAGAC 1 cut(s) 296
BsuRI GGCC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 152, 475
BtsIMutI CAGTG 2 cut(s) 434, 476
CseI GACGC 1 cut(s) 602
Csp6I GTAC 3 cut(s) 534, 553, 579
CviAII CATG 2 cut(s) 26, 128
CviJI RGCY 6 cut(s) 266, 426, 432, 447, 503, 607
CviKI_1 RGCY 6 cut(s) 266, 426, 432, 447, 503, 607
CviQI GTAC 3 cut(s) 534, 553, 579
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 19
Eco57I CTGAAG 1 cut(s) 53
EcoT14I CCWWGG 1 cut(s) 19
ErhI CCWWGG 1 cut(s) 19
FaeI CATG 2 cut(s) 29, 131
FaqI GGGAC 1 cut(s) 313
FatI CATG 2 cut(s) 25, 127
Fnu4HI GCNGC 1 cut(s) 504
FokI GGATG 2 cut(s) 159, 482
Fsp4HI GCNGC 1 cut(s) 504
GluI GCNGC 1 cut(s) 504
GsaI CCCAGC 1 cut(s) 510
HaeIII GGCC 1 cut(s) 447
HgaI GACGC 1 cut(s) 602
Hin1II CATG 2 cut(s) 29, 131
HindIII AAGCTT 1 cut(s) 264
HinfI GANTC 1 cut(s) 473
HphI GGTGA 2 cut(s) 62, 263
Hpy188III TCNNGA 2 cut(s) 71, 497
HpyAV CCTTC 6 cut(s) 23, 28, 542, 557, 568, 583
HpyCH4III ACNGT 6 cut(s) 103, 215, 414, 557, 570, 583
HpyCH4IV ACGT 2 cut(s) 257, 293
HpyCH4V TGCA 3 cut(s) 131, 189, 348
HpySE526I ACGT 2 cut(s) 257, 293
Hsp92II CATG 2 cut(s) 29, 131
Kzo9I GATC 1 cut(s) 493
LmnI GCTCC 1 cut(s) 394
LpnPI CCDG 5 cut(s) 56, 321, 464, 492, 531
Lsp1109I GCAGC 1 cut(s) 490
LweI GCATC 2 cut(s) 176, 511
MaeII ACGT 2 cut(s) 257, 293
MaeIII GTNAC 1 cut(s) 209
MalI GATC 1 cut(s) 495
MboI GATC 1 cut(s) 493
MboII GAAGA 5 cut(s) 59, 283, 293, 301, 398
MhlI GDGCHC 1 cut(s) 518
MluCI AATT 1 cut(s) 405
MlyI GAGTC 1 cut(s) 467
MmeI TCCRAC 1 cut(s) 124
MnlI CCTC 3 cut(s) 210, 346, 594
MseI TTAA 1 cut(s) 332
NdeII GATC 1 cut(s) 493
NlaIII CATG 2 cut(s) 29, 131
NspV TTCGAA 1 cut(s) 175
PkrI GCNGC 1 cut(s) 505
PleI GAGTC 1 cut(s) 467
PpsI GAGTC 1 cut(s) 467
PsiI TTATAA 2 cut(s) 219, 402
PspFI CCCAGC 1 cut(s) 506
RsaI GTAC 3 cut(s) 535, 554, 580
RsaNI GTAC 3 cut(s) 534, 553, 579
SaqAI TTAA 1 cut(s) 332
SatI GCNGC 1 cut(s) 504
Sau3AI GATC 1 cut(s) 493
SchI GAGTC 1 cut(s) 467
SduI GDGCHC 1 cut(s) 518
SetI ASST 9 cut(s) 202, 253, 260, 268, 296, 340, 428, 434, 505
SfaNI GCATC 2 cut(s) 176, 511
SfcI CTRYAG 3 cut(s) 427, 556, 582
SfuI TTCGAA 1 cut(s) 175
SmlI CTYRAG 1 cut(s) 181
SmoI CTYRAG 1 cut(s) 181
Sse9I AATT 1 cut(s) 405
SsiI CCGC 1 cut(s) 16
StyI CCWWGG 1 cut(s) 19
TaaI ACNGT 6 cut(s) 103, 215, 414, 557, 570, 583
TaiI ACGT 2 cut(s) 260, 296
TaqI TCGA 1 cut(s) 175
TaqII GACCGA 1 cut(s) 74
TasI AATT 1 cut(s) 405
TatI WGTACW 1 cut(s) 533
Tru1I TTAA 1 cut(s) 332
Tru9I TTAA 1 cut(s) 332
TscAI CASTG 2 cut(s) 441, 483
TseI GCWGC 1 cut(s) 503
TspDTI ATGAA 5 cut(s) 20, 42, 116, 276, 302
TspRI CASTG 2 cut(s) 441, 483
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.