pycom14g07050

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016934
Physical Location & Seq
Forward (+)
7024076 .. 7026091
2016 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07050.1

Sequence Viewer

Length: 2016 bp
ATGTTCAAATCATGTTTCGTTGATTCGGGTTTTCTCCGAAACTCTCTGTTTCTTGTGGAGAAGGGGTATTTTAGAGCGTATTACGCCACTGCATCTGCGCTGTTGACGACAGATGCCCATGTGTGTGAGGAAAACCCATTGCCGGTTGATGCCTATGTGATTCAGGAGATTAAAGCTGTGAGAGAAAGGTCCAGCATTTGGGAAAAGCGGAAACTTTATCCAGTTGTTACCAGAGTGTTTAAATCTTTGGATTGGGAGGTTGCATGGGACCTGAGGTTCTCCAGGTCTGTGAAGCAGTATGGCTTTTCCAATTCTTTAAGTGCATTTAGTGTTATTGTTCATGTCTTTGCATTGGCGGGGATGAAAATGGAAGTGCAGTCTTTGCTCAGTGATATTGTTTGCTATTATCGAGATGCTAAGTATGATGCATTTGGGTTGTTCCCATATTTATTTGATTCACCTCACAATGGGGTGAGAACTCTTGTATTTGATGCCCTCATTATGACTTTTGCTGCCAACTCGATGCTTGAGAATGCTGTCGATGTTTTTGTGCAGACCAAAAACATGCAGCTTGAACCACACATTGGGTCTTGTAATTTTTTGCTCAAGTGTTTGGCTGAAGCAAACAAATTGGAATCTGTTAGAAGTTTATTCGAATGTTTAAGAAAGTATGGTCCTTCACCTAATGTTTACAGTTACACTATTATGATGAACTTTTACTGCCAAGGACATGAAGGGAAGGGTGTAGATATAGATGAAGCGACTGACATTCTTCAGGAAATGGAGAAGAGTGGGAAACAACCAACTGTTGTGATATACACCAAATATATTCATGCACTTTGTAACGTTGGATGGGTTGAGTTTGCTTTGGACTTTATTCGGAACTTGAGATGCAGAAATCAACCTCTTAATAGTTACTGTTATAACGCCATACTTTTTGGTTTTTGCCAAAATGGCAAAACATATGAAGCTTTGAAGGTTTTGGAAGAAATGAAGACGTATGGGACACTACCAGATGTTTATTGCTATACCATTTTGATAGATGGACTTTGCAAGGAAGGAGATATTGAGATTGCTCTCACTTTGTTTGAAGAAATGGAGCATTATAAAATTAAACCGTCTTTGGTTAGCTATAGCTCACTGATATGTGGTCTTTGTAAGATTGGGTTGGTGGATGACTCATTGGATATTTTTCGTGATCTTGTGGCTGATGGGATGCGCCCTGATGCCATCACTTGCACTCTCATTGTTGAAGGGTACTGTAGGGAAGGACGCTTGAAGGAAGCCTTGAAATTTATGTATGAGATGCACAACCAAGGCATAAACCTTAACTCATATACTTATAATGCAGTCATCAAGAGTTTGTGCAAGGAAAGGCAACCCGAAAAAGCATGGGAATTTTTCCCTCAAATGCTCAAGAGGAATATACATCCAGAAGTTGCAAATTATAATACTCTTATGAATGGTTTTGCAAAGAAGTTAAGTACAAAGAAGGCTTTGATTGTGTATAAAGGAATGCTGAAAGTTGGTGTCATGCCCAATGCAGTAACATATACGATCCTTATTAACACATTCTGCAATGGAGGCAAAATGTGTGAAGCTTATAACTTGTTCAAGGAAATGAGTGAAAGGGGTTTGGTTGTGGATGTCATTCCTTACACGTCTCTAATTGCTGGGTTTTATAGAATTGGAGACATGAAGAAGGCATGGGTGTTGTTCAATGAAATGTTGAGGAATAACCATTTGCCCAATGCAATTACTTATACCTGTTTAATAGATGGGTTTTGCAAGTCCTTCCGCATGGATTGTGCCAGCTTCTTGTTTGACGAAATGAAGAGAAAAAATGTTATTCCCGATGCTGTGACTTACACTGTGCTCATGATTGGGTATTTTAGACTTGGGAATATTGATAGAGCAATTCAGTTATTTGGTGAAATGAAGGAGAGGGGTATTTCCCCAGATGTTATTGCCCAGGAAACTATGGGGCTTTATACGGGCACGCTTACAAAAGGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

672

Amino Acids

76.64

Weight (kDa)

7.06

Isoelectric Point (pI)

31.33

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 193 - 242 1.4e-08 PPR repeat family
PPR_2 PF13041 268 - 316 6.6e-07 PPR repeat family
PPR_long PF17177 297 - 409 1.3e-07 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 303 - 332 1e-07 PPR repeat
PPR_2 PF13041 304 - 352 3.9e-16 PPR repeat family
PPR PF01535 307 - 335 1.7e-06 PPR repeat
PPR_1 PF12854 337 - 367 7.5e-12 PPR repeat
PPR_3 PF13812 338 - 382 5.8e-08 Pentatricopeptide repeat domain
PPR PF01535 342 - 368 4e-07 PPR repeat
PPR_2 PF13041 346 - 387 5.7e-12 PPR repeat family
PPR_1 PF12854 371 - 400 6.4e-07 PPR repeat
PPR_2 PF13041 373 - 422 4.8e-09 PPR repeat family
TPR_24 PF23276 396 - 504 7e-09 Fungal tetratrico peptide repeats
PPR_3 PF13812 397 - 454 1.5e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 404 - 436 1.7e-07 PPR repeat
PPR_2 PF13041 408 - 457 2.4e-14 PPR repeat family
PPR_long PF17177 421 - 570 6.1e-10 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 440 - 471 3.2e-06 PPR repeat
PPR_2 PF13041 444 - 492 9.5e-14 PPR repeat family
PPR PF01535 446 - 476 1.7e-06 PPR repeat
PPR_3 PF13812 470 - 526 2.5e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 478 - 527 1.8e-13 PPR repeat family
PPR_1 PF12854 510 - 542 8.1e-11 PPR repeat
PPR_2 PF13041 522 - 561 7.8e-07 PPR repeat family
PPR_3 PF13812 537 - 593 1.4e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 549 - 597 1.3e-16 PPR repeat family
PPR_1 PF12854 580 - 612 5.5e-10 PPR repeat
PPR_2 PF13041 583 - 631 5.9e-15 PPR repeat family
PPR PF01535 586 - 616 1.2e-06 PPR repeat
PPR_3 PF13812 607 - 656 1.4e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 614 - 647 1.3e-07 PPR repeat
PPR_2 PF13041 618 - 656 4.6e-11 PPR repeat family
PPR PF01535 621 - 651 2.6e-08 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016934)

Species Orthologous Gene IDs
malus_domestica MD14G1081200.v1.1
pyrus_communis pycom12g07360 pycom14g07050
rosa_chinensis RchiOBHm_Chr3g0484571
rosa_laevigata RLG00000023219
rosa_multiflora Rmu_co8455711.1_g000001 Rmu_sc0000295.1_g000038 Rmu_sc0032195.1_g000001
rosa_roxburghii Rroxscaffold_6G00397630
rosa_rugosa Rorug03G0211700
rosa_samantha Rh3AG262500 Rh3BG297800 Rh3CG294800 Rh3DG291100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 5 cut(s) 924, 1107, 1344, 1449, 1605
AccB7I CCANNNNNTGG 2 cut(s) 198, 584
AciI CCGC 3 cut(s) 208, 356, 1798
AclI AACGTT 1 cut(s) 846
AclWI GGATC 1 cut(s) 1552
AcsI RAATTY 2 cut(s) 1292, 1397
AcuI CTGAAG 2 cut(s) 639, 758
AfaI GTAC 2 cut(s) 1259, 1486
AfiI CCNNNNNNNGG 4 cut(s) 142, 198, 467, 584
AflIII ACRYGT 1 cut(s) 1659
AgsI TTSAA 9 cut(s) 7, 575, 976, 1091, 1253, 1279, 1291, 1615, 1720
AjiI CACGTC 1 cut(s) 1662
AjnI CCWGG 2 cut(s) 281, 1971
AjuI GAANNNNNNNTTGG 2 cut(s) 567, 599
AloI GAACNNNNNNTCC 2 cut(s) 260, 292
AluBI AGCT 7 cut(s) 176, 571, 971, 1131, 1137, 1601, 1815
AluI AGCT 7 cut(s) 176, 571, 971, 1131, 1137, 1601, 1815
Alw21I GWGCWC 1 cut(s) 1878
Alw26I GTCTC 2 cut(s) 1668, 1686
AlwI GGATC 1 cut(s) 1552
ApeKI GCWGC 2 cut(s) 512, 568
ApoI RAATTY 2 cut(s) 1292, 1397
AspLEI GCGC 2 cut(s) 100, 1221
AspS9I GGNCC 3 cut(s) 189, 268, 674
AsuHPI GGTGA 4 cut(s) 450, 484, 672, 1943
AsuII TTCGAA 1 cut(s) 654
AvaII GGWCC 3 cut(s) 189, 268, 674
AxyI CCTNAGG 1 cut(s) 272
BaeGI GKGCMC 1 cut(s) 2000
BbsI GAAGAC 1 cut(s) 1001
Bbv12I GWGCWC 1 cut(s) 1878
BbvI GCAGC 2 cut(s) 499, 580
BccI CCATC 5 cut(s) 846, 1037, 1205, 1238, 1772
BciT130I CCWGG 2 cut(s) 283, 1973
BcoDI GTCTC 2 cut(s) 1668, 1686
BfmI CTRYAG 2 cut(s) 1132, 1261
BglI GCCNNNNNGGC 1 cut(s) 954
BisI GCNGC 2 cut(s) 513, 569
BlsI GCNGC 2 cut(s) 514, 570
Bme1390I CCNGG 2 cut(s) 283, 1973
Bme18I GGWCC 3 cut(s) 189, 268, 674
BmgBI CACGTC 1 cut(s) 1662
BmgT120I GGNCC 3 cut(s) 189, 268, 674
BmiI GGNNCC 1 cut(s) 269
BmrFI CCNGG 2 cut(s) 283, 1973
BpiI GAAGAC 1 cut(s) 1001
BpmI CTGGAG 1 cut(s) 265
Bpu14I TTCGAA 1 cut(s) 654
BpuEI CTTGAG 4 cut(s) 548, 590, 907, 1400
BsaJI CCNNGG 3 cut(s) 724, 1315, 1971
BsaXI ACNNNNNCTCC 2 cut(s) 50, 80
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 198, 467, 584
Bse118I RCCGGY 1 cut(s) 142
Bse1I ACTGG 1 cut(s) 221
Bse21I CCTNAGG 1 cut(s) 272
Bse3DI GCAATG 2 cut(s) 137, 1585
BseBI CCWGG 2 cut(s) 283, 1973
BseDI CCNNGG 3 cut(s) 724, 1315, 1971
BseGI GGATG 6 cut(s) 366, 857, 1180, 1221, 1429, 1651
BseLI CCNNNNNNNGG 4 cut(s) 142, 198, 467, 584
BseMI GCAATG 2 cut(s) 137, 1585
BseMII CTCAG 2 cut(s) 263, 400
BseNI ACTGG 1 cut(s) 221
BseSI GKGCMC 1 cut(s) 2000
BseXI GCAGC 2 cut(s) 499, 580
BseYI CCCAGC 1 cut(s) 1673
BsgI GTGCAG 2 cut(s) 395, 572
BsiHKAI GWGCWC 1 cut(s) 1878
BsiSI CCGG 1 cut(s) 143
BslFI GGGAC 2 cut(s) 281, 1018
BslI CCNNNNNNNGG 4 cut(s) 142, 198, 467, 584
BsmAI GTCTC 2 cut(s) 1668, 1686
BsmBI CGTCTC 1 cut(s) 1668
BsmFI GGGAC 2 cut(s) 281, 1018
BsmI GAATGC 2 cut(s) 538, 1521
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 2 cut(s) 1878, 2000
Bsp143I GATC 2 cut(s) 1198, 1557
BspACI CCGC 3 cut(s) 208, 356, 1798
BspCNI CTCAG 2 cut(s) 264, 399
BspHI TCATGA 1 cut(s) 1878
BspLI GGNNCC 1 cut(s) 269
BspPI GGATC 1 cut(s) 1552
BspT104I TTCGAA 1 cut(s) 654
BsrDI GCAATG 2 cut(s) 137, 1585
BsrFI RCCGGY 1 cut(s) 142
BsrI ACTGG 1 cut(s) 221
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 3 cut(s) 724, 1315, 1971
BssMI GATC 2 cut(s) 1198, 1557
BssT1I CCWWGG 2 cut(s) 724, 1315
Bst2UI CCWGG 2 cut(s) 283, 1973
Bst4CI ACNGT 6 cut(s) 695, 808, 920, 1119, 1262, 1873
Bst6I CTCTTC 2 cut(s) 782, 1829
BstAPI GCANNNNNTGC 1 cut(s) 382
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 2 cut(s) 1813, 2000
BstDEI CTNAG 3 cut(s) 272, 386, 417
BstF5I GGATG 6 cut(s) 366, 857, 1180, 1221, 1429, 1651
BstHHI GCGC 2 cut(s) 100, 1221
BstKTI GATC 2 cut(s) 1201, 1560
BstMAI GTCTC 2 cut(s) 1668, 1686
BstMBI GATC 2 cut(s) 1198, 1557
BstMWI GCNNNNNNNGC 4 cut(s) 83, 382, 954, 1584
BstNI CCWGG 2 cut(s) 283, 1973
BstNSI RCATGY 1 cut(s) 568
BstSCI CCNGG 2 cut(s) 281, 1971
BstSFI CTRYAG 2 cut(s) 1132, 1261
BstSLI GKGCMC 1 cut(s) 2000
BstV1I GCAGC 2 cut(s) 499, 580
BstV2I GAAGAC 1 cut(s) 1001
Bsu36I CCTNAGG 1 cut(s) 272
BtrI CACGTC 1 cut(s) 1662
BtsCI GGATG 6 cut(s) 366, 857, 1180, 1221, 1429, 1651
BtsI GCAGTG 1 cut(s) 87
BtsIMutI CAGTG 4 cut(s) 87, 394, 1139, 1869
Cac8I GCNNGC 2 cut(s) 1813, 2000
CciI TCATGA 1 cut(s) 1878
CfoI GCGC 2 cut(s) 100, 1221
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 3 cut(s) 189, 268, 674
CseI GACGC 1 cut(s) 1281
Csp6I GTAC 2 cut(s) 1258, 1485
CviQI GTAC 2 cut(s) 1258, 1485
DdeI CTNAG 3 cut(s) 272, 386, 417
DpnI GATC 2 cut(s) 1200, 1559
DpnII GATC 2 cut(s) 1198, 1557
DraI TTTAAA 1 cut(s) 241
Eam1104I CTCTTC 2 cut(s) 782, 1829
EarI CTCTTC 2 cut(s) 782, 1829
Eco130I CCWWGG 2 cut(s) 724, 1315
Eco47I GGWCC 3 cut(s) 189, 268, 674
Eco57I CTGAAG 2 cut(s) 639, 758
Eco81I CCTNAGG 1 cut(s) 272
EcoO109I RGGNCCY 1 cut(s) 268
EcoRII CCWGG 2 cut(s) 281, 1971
EcoT14I CCWWGG 2 cut(s) 724, 1315
EcoT22I ATGCAT 1 cut(s) 430
ErhI CCWWGG 2 cut(s) 724, 1315
Esp3I CGTCTC 1 cut(s) 1668
FalI AAGNNNNNCTT 2 cut(s) 1271, 1303
FaqI GGGAC 2 cut(s) 281, 1018
FauI CCCGC 1 cut(s) 349
FauNDI CATATG 1 cut(s) 964
Fnu4HI GCNGC 2 cut(s) 513, 569
FokI GGATG 6 cut(s) 373, 864, 1187, 1228, 1416, 1658
Fsp4HI GCNGC 2 cut(s) 513, 569
GlaI GCGC 2 cut(s) 99, 1220
GluI GCNGC 2 cut(s) 513, 569
GsaI CCCAGC 1 cut(s) 1677
GsuI CTGGAG 1 cut(s) 265
HapII CCGG 1 cut(s) 143
HgaI GACGC 1 cut(s) 1281
HhaI GCGC 2 cut(s) 100, 1221
Hin6I GCGC 2 cut(s) 98, 1219
HinP1I GCGC 2 cut(s) 98, 1219
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HindIII AAGCTT 2 cut(s) 969, 1599
HinfI GANTC 5 cut(s) 23, 160, 455, 635, 1178
HpaII CCGG 1 cut(s) 143
HphI GGTGA 4 cut(s) 450, 484, 672, 1943
Hpy166II GTNNAC 2 cut(s) 105, 691
Hpy188I TCNGA 2 cut(s) 38, 882
Hpy188III TCNNGA 9 cut(s) 164, 410, 776, 1196, 1357, 1417, 1433, 1853, 1879
Hpy8I GTNNAC 2 cut(s) 105, 691
HpyCH4III ACNGT 6 cut(s) 695, 808, 920, 1119, 1262, 1873
HpyCH4IV ACGT 3 cut(s) 846, 998, 1661
HpyF10VI GCNNNNNNNGC 4 cut(s) 83, 382, 954, 1584
HpyF3I CTNAG 3 cut(s) 272, 386, 417
HpySE526I ACGT 3 cut(s) 846, 998, 1661
HspAI GCGC 2 cut(s) 98, 1219
Kzo9I GATC 2 cut(s) 1198, 1557
LmnI GCTCC 1 cut(s) 1099
Lsp1109I GCAGC 2 cut(s) 499, 580
MaeII ACGT 3 cut(s) 846, 998, 1661
MaeIII GTNAC 6 cut(s) 226, 695, 842, 914, 1546, 1861
MalI GATC 2 cut(s) 1200, 1559
MboI GATC 2 cut(s) 1198, 1557
MboII GAAGA 7 cut(s) 764, 799, 998, 1006, 1103, 1711, 1846
MhlI GDGCHC 2 cut(s) 1878, 2000
MlyI GAGTC 1 cut(s) 1172
MmeI TCCRAC 1 cut(s) 829
Mph1103I ATGCAT 1 cut(s) 430
MslI CAYNNNNRTG 3 cut(s) 123, 704, 1144
MspI CCGG 1 cut(s) 143
MspR9I CCNGG 2 cut(s) 283, 1973
Mva1269I GAATGC 2 cut(s) 538, 1521
MvaI CCWGG 2 cut(s) 283, 1973
MwoI GCNNNNNNNGC 4 cut(s) 83, 382, 954, 1584
NdeI CATATG 1 cut(s) 964
NdeII GATC 2 cut(s) 1198, 1557
NlaIV GGNNCC 1 cut(s) 269
NmuCI GTSAC 1 cut(s) 1861
NsiI ATGCAT 1 cut(s) 430
NspI RCATGY 1 cut(s) 568
NspV TTCGAA 1 cut(s) 654
PagI TCATGA 1 cut(s) 1878
PctI GAATGC 2 cut(s) 538, 1521
PfeI GAWTC 4 cut(s) 23, 160, 455, 635
PflMI CCANNNNNTGG 2 cut(s) 198, 584
PkrI GCNGC 2 cut(s) 514, 570
PleI GAGTC 1 cut(s) 1172
PpsI GAGTC 1 cut(s) 1172
PpuMI RGGWCCY 1 cut(s) 268
PsiI TTATAA 5 cut(s) 924, 1107, 1344, 1449, 1605
Psp1406I AACGTT 1 cut(s) 846
Psp5II RGGWCCY 1 cut(s) 268
Psp6I CCWGG 2 cut(s) 281, 1971
PspFI CCCAGC 1 cut(s) 1673
PspGI CCWGG 2 cut(s) 281, 1971
PspN4I GGNNCC 1 cut(s) 269
PspPI GGNCC 3 cut(s) 189, 268, 674
PspPPI RGGWCCY 1 cut(s) 268
RsaI GTAC 2 cut(s) 1259, 1486
RsaNI GTAC 2 cut(s) 1258, 1485
RseI CAYNNNNRTG 3 cut(s) 123, 704, 1144
SatI GCNGC 2 cut(s) 513, 569
Sau3AI GATC 2 cut(s) 1198, 1557
Sau96I GGNCC 3 cut(s) 189, 268, 674
SchI GAGTC 1 cut(s) 1172
ScrFI CCNGG 2 cut(s) 283, 1973
SduI GDGCHC 2 cut(s) 1878, 2000
SfcI CTRYAG 2 cut(s) 1132, 1261
SfuI TTCGAA 1 cut(s) 654
SinI GGWCC 3 cut(s) 189, 268, 674
SmiMI CAYNNNNRTG 3 cut(s) 123, 704, 1144
SmlI CTYRAG 4 cut(s) 527, 605, 886, 1415
SmoI CTYRAG 4 cut(s) 527, 605, 886, 1415
SsiI CCGC 3 cut(s) 208, 356, 1798
SspI AATATT 1 cut(s) 1906
StyD4I CCNGG 2 cut(s) 281, 1971
StyI CCWWGG 2 cut(s) 724, 1315
TaaI ACNGT 6 cut(s) 695, 808, 920, 1119, 1262, 1873
TaiI ACGT 3 cut(s) 849, 1001, 1664
TaqI TCGA 4 cut(s) 409, 521, 540, 654
TatI WGTACW 1 cut(s) 1484
TfiI GAWTC 4 cut(s) 23, 160, 455, 635
TscAI CASTG 4 cut(s) 94, 394, 1146, 1876
TseFI GTSAC 1 cut(s) 1861
TseI GCWGC 2 cut(s) 512, 568
Tsp45I GTSAC 1 cut(s) 1861
TspRI CASTG 4 cut(s) 94, 394, 1146, 1876
Van91I CCANNNNNTGG 2 cut(s) 198, 584
VpaK11BI GGWCC 3 cut(s) 189, 268, 674
XapI RAATTY 2 cut(s) 1292, 1397
XceI RCATGY 1 cut(s) 568
Zsp2I ATGCAT 1 cut(s) 430
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.