RchiOBHm_Chr3g0484571

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0016934
Physical Location & Seq
Forward (+)
30941085 .. 30946722
5638 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ44928

Sequence Viewer

Length: 2031 bp
ATGTTCAAATCATGTTTGGTTGATTCGGCTTTTCTCCGAAACTGTGTGTTTTTTGTCAATAGGGTGAGTTTTAGAGTGTATTCTGCCGCTGCTTCTGCATTGTTGCTGGAGGATCATGTGTTTGTGAAAGCTCCATTACCCTTTGAGGCCTTTGCAGTTCAGGAAATTAAAGCTCTGGGGAAAAGGTTTTGTACTCAGAGAAAGAAAAATTTGTATCCCTTTGTTGTCAGATTGTTTAAAACTTTGAATTGGGAGGTCGCATGGGATGCAAGGTTCTCTAAGTCTGTGAAAGAGCATGGCTTTTCTCATTCTTTAAATGCCTTTAGAATTATTGTTCATGAATTTGCATTGGTGGGAATGCAAATGGAAGTGCAGGCTTTGCTTAGAGATGTTGTATTCTATTATCAAGAAGCTAAGTATGATGCATTTGAGCTGTTCCCATATTTATTGGATTCACCTCACAGTGGGGTGAGATCGCTTGTTGTATTTGATGCACTAATAACGGCTTTCGCTGACAACTCGATGCCTGAGAATGCTGTGGATGTGTTTTTGAAGATTAAAAGAATGGGTCTTGAGCCACACATTTGGTCTTGTAATATCTTGCTCAAGTGTTTGGCAGAAGCAAACAAACTGGAATGTGTTAGAAGCTTATTCGAATGTTTGAAAAACTCTGGGCCATCTCCTAATGTTTACACTTACACAGTTATGATGAGCTTTTTCTGTACAGGATATCCACTGCAGGATGTGGATATCAGTCGAGCGACTGACATTCTTCAAGAAATGGAGAAAAGTGGGAAACACCCAACTGCAGTGATATATGCTGAATATATTCATGGACTTTGTAAAGCTGGATATGTTGAGTTTGCTTTGGACTTCATCCGAAACATGCAACACAGAGACCAACCTCTCAATAGTTATTGTTATAATGCTATACTTCGTGGGTTTTGCCAAAAAGGCGAAACTTGTGAAGCTCTAAAATTGTTGGAGGAAATGAAAAGCTACGGGATGGTGCCAGATGTTTATTGCTACACCATATTGATAGATGGATTTTGCAAGGAAGGGGATATAGAGAAAGCTCTTGCTTTGTTTGAGGAAATGGAGCTTTGTAAAATAAAACCTTCTTTGGTTAGCTATAGCTCACTCATATATGGTCTTTGCAAACTTGGATTGATAGATCATATGTTAGATATATATCGTAATCTGGAGTCTGCCGGTGTGTGCCCTGATGCTATCACTTGCACTCTCATTGTTGATGGGTTTTGCAGGGAAGGGCGCTTGGAGGAAGCCTTGAGATTTATTTACAAGTTGTATCATCAAGGCATAAGCCCTAACTCGTATACTTATAATGCAGTCCTCAACAGTCTGTGCAAGCAAGGAAAGCCAGAAAAAGCATGGGAACTAATCCCTCAAATGCTTAAAAGGAATATACTCCCAGAAGTTGCAAATTATAATACTCTTATAAATGGCTTTGGAAAACAGTTGAATTCAAAGAAGGCCTGCATGGTGTATGGAGGAATGCTCAAAGCTGGTGTCATGCCTAATACAGTCACATATACAATCCTTATCAATATACTCTCCCATAGAGGCAAAATATATGAAGCTTGTAATATGTTCAAGGAAATGAGTGAAAGGGGTTTGGCTGCAGATGTGATTTCTTATACATCTCTAATTGCTGGGTTTTGTAGGATGGGAGACATGAAGAAGGCTTGGGTACTGTTCAAAGAAATGTTGAGCAAAAACCATTTGCCCAATGCTATCACGTATACGTGTTTAATAGATGGTTTTTGCAAGTCTTCTCGCATGGATTATGCCAGTTTCTTGTTTGAGGAAATGAAGAATAAAAATGTTACTCCTGATCTGGTGACTTACACTGTTCTCATCATTGGGTACTGTAGGCTCCGGAATATTGATAGAGCAATTCAGTTATTTGATGAAATGAAGCACAGTGGTATCTCAGGGGATGGTTTTGCCAATGAAGCTTTGGGGCTTTACACCAAAGACTGGTTTGTTTACAAAAGATTAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PPR PPR_1 PPR_2 PPR_3

Protein Analysis

676

Amino Acids

77.08

Weight (kDa)

7.86

Isoelectric Point (pI)

36.86

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 194 - 241 1.7e-08 PPR repeat family
PPR_2 PF13041 268 - 317 5.1e-09 PPR repeat family
PPR_long PF17177 285 - 410 1.3e-08 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 303 - 332 2.8e-07 PPR repeat
PPR_2 PF13041 304 - 352 3.4e-17 PPR repeat family
MRP-S27 PF10037 309 - 366 2.2e-06 Mitochondrial 28S ribosomal protein S27
PPR_3 PF13812 326 - 382 2e-10 Pentatricopeptide repeat domain
PPR_1 PF12854 335 - 367 2.5e-14 PPR repeat
PPR PF01535 342 - 369 1.1e-08 PPR repeat
PPR_2 PF13041 343 - 387 3.1e-14 PPR repeat family
PPR_2 PF13041 373 - 422 4.6e-09 PPR repeat family
TPR_24 PF23276 396 - 504 1.6e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 397 - 454 3.1e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 405 - 432 3.8e-07 PPR repeat
PPR_2 PF13041 408 - 457 8.3e-13 PPR repeat family
PPR_1 PF12854 440 - 471 4.3e-08 PPR repeat
PPR PF01535 446 - 476 1.6e-06 PPR repeat
PPR_2 PF13041 447 - 478 4e-08 PPR repeat family
TPR_24 PF23276 450 - 576 2.2e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 470 - 525 9.2e-07 Pentatricopeptide repeat domain
PPR_2 PF13041 478 - 525 1.7e-12 PPR repeat family
PPR_1 PF12854 510 - 542 6.5e-07 PPR repeat
PPR_2 PF13041 513 - 562 1.2e-14 PPR repeat family
PPR PF01535 516 - 546 1.7e-06 PPR repeat
TPR_24 PF23276 526 - 645 1.3e-07 Fungal tetratrico peptide repeats
PPR_3 PF13812 537 - 593 8.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 544 - 576 1.8e-10 PPR repeat
PPR_2 PF13041 549 - 597 4.9e-19 PPR repeat family
PPR PF01535 551 - 580 3.2e-08 PPR repeat
PPR_long PF17177 570 - 652 7.5e-09 Pentacotripeptide-repeat region of PRORP
PPR_1 PF12854 579 - 612 2e-10 PPR repeat
PPR_2 PF13041 583 - 632 6.4e-17 PPR repeat family
PPR PF01535 586 - 616 5.6e-07 PPR repeat
PPR_3 PF13812 607 - 652 1.8e-08 Pentatricopeptide repeat domain
PPR_1 PF12854 614 - 647 4.3e-11 PPR repeat
PPR PF01535 621 - 651 9.7e-09 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0016934)

Species Orthologous Gene IDs
malus_domestica MD14G1081200.v1.1
pyrus_communis pycom12g07360 pycom14g07050
rosa_chinensis RchiOBHm_Chr3g0484571
rosa_laevigata RLG00000023219
rosa_multiflora Rmu_co8455711.1_g000001 Rmu_sc0000295.1_g000038 Rmu_sc0032195.1_g000001
rosa_roxburghii Rroxscaffold_6G00397630
rosa_rugosa Rorug03G0211700
rosa_samantha Rh3AG262500 Rh3BG297800 Rh3CG294800 Rh3DG291100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 4 cut(s) 924, 1344, 1449, 1460
AccB1I GGYRCC 1 cut(s) 1009
AccB7I CCANNNNNTGG 1 cut(s) 2001
AccI GTMKAC 2 cut(s) 1337, 1763
AccIII TCCGGA 1 cut(s) 1899
AciI CCGC 1 cut(s) 87
AclWI GGATC 1 cut(s) 120
AcsI RAATTY 3 cut(s) 208, 341, 1483
AfaI GTAC 4 cut(s) 193, 724, 1713, 1889
AfiI CCNNNNNNNGG 2 cut(s) 464, 2001
AflIII ACRYGT 1 cut(s) 1766
AgsI TTSAA 9 cut(s) 7, 247, 553, 664, 776, 1483, 1488, 1615, 1720
AloI GAACNNNNNNTCC 2 cut(s) 257, 289
Alw26I GTCTC 2 cut(s) 891, 1686
AlwI GGATC 1 cut(s) 120
Aor13HI TCCGGA 1 cut(s) 1899
AoxI GGCC 3 cut(s) 147, 674, 1494
ApeKI GCWGC 2 cut(s) 89, 1640
ApoI RAATTY 3 cut(s) 208, 341, 1483
ArsI GACNNNNNNTTYG 2 cut(s) 1515, 1547
Asp700I GAANNNNTTC 1 cut(s) 828
AspLEI GCGC 1 cut(s) 1275
AspS9I GGNCC 1 cut(s) 674
AsuHPI GGTGA 4 cut(s) 76, 447, 481, 1873
AsuII TTCGAA 1 cut(s) 654
BaeGI GKGCMC 1 cut(s) 1223
BaeI ACNNNNGTAYC 2 cut(s) 1933, 1966
BanI GGYRCC 1 cut(s) 1009
BbsI GAAGAC 1 cut(s) 1785
BbvI GCAGC 2 cut(s) 76, 1627
BccI CCATC 7 cut(s) 685, 1000, 1037, 1247, 1681, 1772, 1955
BceAI ACGGC 1 cut(s) 519
BciVI GTATCC 1 cut(s) 225
BcoDI GTCTC 2 cut(s) 891, 1686
BfmI CTRYAG 5 cut(s) 737, 807, 1132, 1641, 1891
BfoI RGCGCY 1 cut(s) 1276
BfuI GTATCC 1 cut(s) 225
BglI GCCNNNNNGGC 1 cut(s) 954
BisI GCNGC 3 cut(s) 87, 90, 1641
BlsI GCNGC 3 cut(s) 88, 91, 1642
BmgT120I GGNCC 1 cut(s) 674
BmiI GGNNCC 2 cut(s) 1011, 1898
BmsI GCATC 5 cut(s) 256, 412, 481, 513, 1216
BpiI GAAGAC 1 cut(s) 1785
BplI GAGNNNNNCTC 2 cut(s) 1503, 1535
BpmI CTGGAG 2 cut(s) 128, 1223
Bpu14I TTCGAA 1 cut(s) 654
BpuEI CTTGAG 3 cut(s) 590, 593, 1309
BsaAI YACGTR 2 cut(s) 1761, 1767
BsaBI GATNNNNATC 1 cut(s) 1191
BsaI GGTCTC 1 cut(s) 891
BsaWI WCCGGW 1 cut(s) 1899
Bsc4I CCNNNNNNNGG 2 cut(s) 464, 2001
Bse118I RCCGGY 1 cut(s) 1211
Bse1I ACTGG 3 cut(s) 636, 1812, 2006
Bse8I GATNNNNATC 1 cut(s) 1191
BseAI TCCGGA 1 cut(s) 1899
BseGI GGATG 7 cut(s) 271, 547, 748, 876, 1011, 1692, 1966
BseJI GATNNNNATC 1 cut(s) 1191
BseLI CCNNNNNNNGG 2 cut(s) 464, 2001
BseMII CTCAG 3 cut(s) 209, 519, 1968
BseNI ACTGG 3 cut(s) 636, 1812, 2006
BseSI GKGCMC 1 cut(s) 1223
BseXI GCAGC 2 cut(s) 76, 1627
BseYI CCCAGC 1 cut(s) 1673
BsgI GTGCAG 1 cut(s) 392
BshFI GGCC 3 cut(s) 149, 676, 1496
BshNI GGYRCC 1 cut(s) 1009
BsiSI CCGG 2 cut(s) 1212, 1900
BslI CCNNNNNNNGG 2 cut(s) 464, 2001
BsmAI GTCTC 2 cut(s) 891, 1686
BsmI GAATGC 3 cut(s) 363, 538, 1521
BsnI GGCC 3 cut(s) 149, 676, 1496
Bso31I GGTCTC 1 cut(s) 891
Bsp119I TTCGAA 1 cut(s) 654
Bsp1286I GDGCHC 1 cut(s) 1223
Bsp13I TCCGGA 1 cut(s) 1899
Bsp1407I TGTACA 1 cut(s) 722
Bsp143I GATC 4 cut(s) 112, 473, 1174, 1855
BspACI CCGC 1 cut(s) 87
BspANI GGCC 3 cut(s) 149, 676, 1496
BspCNI CTCAG 3 cut(s) 208, 520, 1967
BspEI TCCGGA 1 cut(s) 1899
BspHI TCATGA 1 cut(s) 337
BspLI GGNNCC 2 cut(s) 1011, 1898
BspMAI CTGCAG 3 cut(s) 741, 811, 1645
BspPI GGATC 1 cut(s) 120
BspT104I TTCGAA 1 cut(s) 654
BspT107I GGYRCC 1 cut(s) 1009
BspTNI GGTCTC 1 cut(s) 891
BsrFI RCCGGY 1 cut(s) 1211
BsrGI TGTACA 1 cut(s) 722
BsrI ACTGG 3 cut(s) 636, 1812, 2006
BssAI RCCGGY 1 cut(s) 1211
BssMI GATC 4 cut(s) 112, 473, 1174, 1855
BssNAI GTATAC 2 cut(s) 1338, 1764
Bst1107I GTATAC 2 cut(s) 1338, 1764
BstAPI GCANNNNNTGC 2 cut(s) 266, 379
BstAUI TGTACA 1 cut(s) 722
BstBAI YACGTR 2 cut(s) 1761, 1767
BstBI TTCGAA 1 cut(s) 654
BstC8I GCNNGC 3 cut(s) 375, 1370, 1498
BstDEI CTNAG 6 cut(s) 195, 279, 383, 414, 528, 1954
BstF5I GGATG 7 cut(s) 271, 547, 748, 876, 1011, 1692, 1966
BstH2I RGCGCY 1 cut(s) 1276
BstHHI GCGC 1 cut(s) 1275
BstKTI GATC 4 cut(s) 115, 476, 1177, 1858
BstMAI GTCTC 2 cut(s) 891, 1686
BstMBI GATC 4 cut(s) 112, 473, 1174, 1855
BstMWI GCNNNNNNNGC 6 cut(s) 95, 266, 379, 954, 1378, 1976
BstNSI RCATGY 1 cut(s) 889
BstSFI CTRYAG 5 cut(s) 737, 807, 1132, 1641, 1891
BstSLI GKGCMC 1 cut(s) 1223
BstV1I GCAGC 2 cut(s) 76, 1627
BstV2I GAAGAC 1 cut(s) 1785
BstXI CCANNNNNNTGG 1 cut(s) 585
BstZ17I GTATAC 2 cut(s) 1338, 1764
BsuI GTATCC 1 cut(s) 225
BsuRI GGCC 3 cut(s) 149, 676, 1496
BtsCI GGATG 7 cut(s) 271, 547, 748, 876, 1011, 1692, 1966
BtsI GCAGTG 2 cut(s) 734, 816
BtsIMutI CAGTG 5 cut(s) 469, 734, 816, 1869, 1951
Cac8I GCNNGC 3 cut(s) 375, 1370, 1498
CciI TCATGA 1 cut(s) 337
CfoI GCGC 1 cut(s) 1275
Cfr10I RCCGGY 1 cut(s) 1211
Cfr13I GGNCC 1 cut(s) 674
Csp6I GTAC 4 cut(s) 192, 723, 1712, 1888
CviQI GTAC 4 cut(s) 192, 723, 1712, 1888
DdeI CTNAG 6 cut(s) 195, 279, 383, 414, 528, 1954
DpnI GATC 4 cut(s) 114, 475, 1176, 1857
DpnII GATC 4 cut(s) 112, 473, 1174, 1855
DraI TTTAAA 2 cut(s) 238, 315
Eco147I AGGCCT 2 cut(s) 149, 1496
Eco31I GGTCTC 1 cut(s) 891
Eco32I GATATC 2 cut(s) 731, 751
EcoRI GAATTC 1 cut(s) 1483
EcoRV GATATC 2 cut(s) 731, 751
EcoT22I ATGCAT 1 cut(s) 427
FauNDI CATATG 1 cut(s) 1179
FblI GTMKAC 2 cut(s) 1337, 1763
Fnu4HI GCNGC 3 cut(s) 87, 90, 1641
FokI GGATG 7 cut(s) 278, 554, 755, 863, 1018, 1699, 1973
Fsp4HI GCNGC 3 cut(s) 87, 90, 1641
GlaI GCGC 1 cut(s) 1274
GluI GCNGC 3 cut(s) 87, 90, 1641
GsaI CCCAGC 1 cut(s) 1677
GsuI CTGGAG 2 cut(s) 128, 1223
HaeII RGCGCY 1 cut(s) 1276
HaeIII GGCC 3 cut(s) 149, 676, 1496
HapII CCGG 2 cut(s) 1212, 1900
HhaI GCGC 1 cut(s) 1275
Hin6I GCGC 1 cut(s) 1273
HinP1I GCGC 1 cut(s) 1273
HindIII AAGCTT 3 cut(s) 646, 1599, 1977
HinfI GANTC 3 cut(s) 23, 452, 1205
HpaII CCGG 2 cut(s) 1212, 1900
HphI GGTGA 4 cut(s) 76, 447, 481, 1873
Hpy166II GTNNAC 4 cut(s) 691, 1338, 1764, 2011
Hpy188I TCNGA 4 cut(s) 38, 198, 230, 881
Hpy188III TCNNGA 8 cut(s) 161, 338, 407, 572, 776, 1202, 1853, 1900
Hpy8I GTNNAC 4 cut(s) 691, 1338, 1764, 2011
HpyAV CCTTC 5 cut(s) 1052, 1128, 1262, 1486, 1696
HpyCH4IV ACGT 2 cut(s) 1760, 1766
HpyF10VI GCNNNNNNNGC 6 cut(s) 95, 266, 379, 954, 1378, 1976
HpyF3I CTNAG 6 cut(s) 195, 279, 383, 414, 528, 1954
HpySE526I ACGT 2 cut(s) 1760, 1766
HspAI GCGC 1 cut(s) 1273
Kpn2I TCCGGA 1 cut(s) 1899
Kzo9I GATC 4 cut(s) 112, 473, 1174, 1855
LmnI GCTCC 3 cut(s) 136, 1099, 1902
Lsp1109I GCAGC 2 cut(s) 76, 1627
LweI GCATC 5 cut(s) 256, 412, 481, 513, 1216
MaeII ACGT 2 cut(s) 1760, 1766
MaeIII GTNAC 3 cut(s) 1546, 1846, 1861
MalI GATC 4 cut(s) 114, 475, 1176, 1857
MboI GATC 4 cut(s) 112, 473, 1174, 1855
MboII GAAGA 5 cut(s) 565, 764, 1711, 1785, 1846
MhlI GDGCHC 1 cut(s) 1223
MlyI GAGTC 1 cut(s) 1214
MmeI TCCRAC 1 cut(s) 963
Mph1103I ATGCAT 1 cut(s) 427
MroI TCCGGA 1 cut(s) 1899
MroXI GAANNNNTTC 1 cut(s) 828
MseI TTAA 6 cut(s) 168, 237, 314, 558, 1416, 1772
MslI CAYNNNNRTG 1 cut(s) 704
MspA1I CMGCKG 1 cut(s) 89
MspI CCGG 2 cut(s) 1212, 1900
Mva1269I GAATGC 3 cut(s) 363, 538, 1521
MwoI GCNNNNNNNGC 6 cut(s) 95, 266, 379, 954, 1378, 1976
NdeI CATATG 1 cut(s) 1179
NdeII GATC 4 cut(s) 112, 473, 1174, 1855
NlaIV GGNNCC 2 cut(s) 1011, 1898
NmuCI GTSAC 2 cut(s) 1546, 1861
NsiI ATGCAT 1 cut(s) 427
NspI RCATGY 1 cut(s) 889
NspV TTCGAA 1 cut(s) 654
PagI TCATGA 1 cut(s) 337
PceI AGGCCT 2 cut(s) 149, 1496
PctI GAATGC 3 cut(s) 363, 538, 1521
PdmI GAANNNNTTC 1 cut(s) 828
PfeI GAWTC 2 cut(s) 23, 452
PflMI CCANNNNNTGG 1 cut(s) 2001
PkrI GCNGC 3 cut(s) 88, 91, 1642
PleI GAGTC 1 cut(s) 1213
PpsI GAGTC 1 cut(s) 1213
Ppu21I YACGTR 2 cut(s) 1761, 1767
PsiI TTATAA 4 cut(s) 924, 1344, 1449, 1460
PspFI CCCAGC 1 cut(s) 1673
PspN4I GGNNCC 2 cut(s) 1011, 1898
PspPI GGNCC 1 cut(s) 674
PstI CTGCAG 3 cut(s) 741, 811, 1645
RsaI GTAC 4 cut(s) 193, 724, 1713, 1889
RsaNI GTAC 4 cut(s) 192, 723, 1712, 1888
RseI CAYNNNNRTG 1 cut(s) 704
SaqAI TTAA 6 cut(s) 168, 237, 314, 558, 1416, 1772
SatI GCNGC 3 cut(s) 87, 90, 1641
Sau3AI GATC 4 cut(s) 112, 473, 1174, 1855
Sau96I GGNCC 1 cut(s) 674
SchI GAGTC 1 cut(s) 1214
SduI GDGCHC 1 cut(s) 1223
SfaNI GCATC 5 cut(s) 256, 412, 481, 513, 1216
SfcI CTRYAG 5 cut(s) 737, 807, 1132, 1641, 1891
SfuI TTCGAA 1 cut(s) 654
SmiMI CAYNNNNRTG 1 cut(s) 704
SmlI CTYRAG 3 cut(s) 572, 605, 1288
SmoI CTYRAG 3 cut(s) 572, 605, 1288
SseBI AGGCCT 2 cut(s) 149, 1496
SsiI CCGC 1 cut(s) 87
SspI AATATT 1 cut(s) 1906
StuI AGGCCT 2 cut(s) 149, 1496
TaiI ACGT 2 cut(s) 1763, 1769
TaqI TCGA 3 cut(s) 521, 654, 757
TatI WGTACW 2 cut(s) 191, 722
TauI GCSGC 1 cut(s) 89
TfiI GAWTC 2 cut(s) 23, 452
Tru1I TTAA 6 cut(s) 168, 237, 314, 558, 1416, 1772
Tru9I TTAA 6 cut(s) 168, 237, 314, 558, 1416, 1772
TscAI CASTG 5 cut(s) 469, 741, 816, 1876, 1951
TseFI GTSAC 2 cut(s) 1546, 1861
TseI GCWGC 2 cut(s) 89, 1640
Tsp45I GTSAC 2 cut(s) 1546, 1861
TspRI CASTG 5 cut(s) 469, 741, 816, 1876, 1951
Van91I CCANNNNNTGG 1 cut(s) 2001
XapI RAATTY 3 cut(s) 208, 341, 1483
XceI RCATGY 1 cut(s) 889
XcmI CCANNNNNNNNNTGG 2 cut(s) 1389, 1978
XmiI GTMKAC 2 cut(s) 1337, 1763
XmnI GAANNNNTTC 1 cut(s) 828
Zsp2I ATGCAT 1 cut(s) 427
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.