pycom12g09760

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012382
Physical Location & Seq
Forward (+)
11636860 .. 11639510
2651 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g09760.1

Sequence Viewer

Length: 2049 bp
ATGAAGAAAAACAAGCCTTTTGCGTTCCTCACTCTCTCACTTCTTCTCTTGGCCTCCGCCCACTCCTCATTCTCATCTCCGCCACCTAACGACACCTGGGCCCTCGACCAATTCCGCCTTCAGACCGACAGCCACGGCTACCTCCGCTCCAACTGGACCGGCTCCGATGCATGCACCCCCGGCTGGGCTGGAGTCCGATGCTCCACCAATAAAGACCGCGTAGTCGCTCTCTTCCTCCCTTCCCTCAACCTCCGGGGCCCACTCGACTCCCTCGCTTCTCTCGACCAGCTCCGCCTCCTCGACCTCCACAACAACCGCCTCAATGGCACCGTTTCTCCTCTCGTCAACTGCACCAAGCTCAAGCTCCTCTACCTCGCAGGCAACGACCTCTCCGGAGAAATTCCGTCGGAGATCTCCTCCCTCCGGCGACTCCTCCGCCTTGACCTCTCCGACAATAACCTCCGGGGACCCGTTCCAGACAATCTCACCCACCTGACCAGGCTCCTAACGCTCCGCCTCCAGAACAACGCCCTCTCCGGCGAAGTCCCCGACCTCTCCGCCTCCCTTGCCGACCTCAAGGAGCTCAATTTCACCAACAACGAATTGTACGGAAGGCTGCCGGAGGGTCTGTTGAAGAAGTTCGGCGATGAAAGCTTTTCCGGGAATGAAGGGCTATGCGGGCCGAGCCCCTTGCCGGCTTGTTCATTCACCGGCACTCGCGATCCGCCCTCGGCGGCTTCATCCGAAACGGTGCCGTCAAATCCAAGCCAATTGCCTCAGACGACGAGCCCAAACGAGCCGAACAAGAAGCAACGGAGGAAGGGGCTGAGCCCCGGCGCAATCGTTGCGATCGTGATCGCGAATTGCGTGGCCATGCTGGTGGTCGTGTCATTCATCGTGGCCCACTACTGCGCGAGAGACCGCGGCGGCTCGAGTTCGATGGCCGGGAGCGAGAGCGGTAAGAGAAGGAGCGGGAGCAGCTACGGAGGCGATCAGAAGAAGGTGTACGCGAACAGCGGGGGTGGCGGGGACAGTGACGGTACAAACGCAACGGACAGGAGCAAGCTGGTATTTTTCGACCGGAGGAAGCAGTTTGAGCTGGAGGATTTGCTGCGGGCGTCGGCGGAGATGTTGGGGAAAGGAAGCCTAGGGACGGTGTACAAGGCGGTGCTGGACGACGGGAGCACCATGGCTGTGAAGCGGCTCAAGGACGCGAACCCGTGCGAGAGGAAGGAGTTTGAGCAGTACATGGATGTGATTGGAAAGGTGAAGCATCCCAATGTGGTGAGGCTGAGTGCTTATTACTATGCCAAGGAAGAGAAGCTTCTTGTCTATGATTATCTCCCTAATGGAAGCTTGCACTCACTTCTGCATGGGAATCGGGGTCCGGGTAGAATTCCATTGGATTGGACTACAAGGATCAGCTTGGTGTTGGGTGCGGCTCGTGGCCTTGCCAGGATTCATGCGGAATACAGCTCTGCTAAAGTACCGCATGGCAATGTCAAGTCTTCCAATGTCCTTCTTGACAAGAATGGCGTCGCTTGCATTTCCGACTTCGGCCTCTCTCTGCTTCTCAACCCGGTTCACGCCATTGCAAGATTGGGAGGATACCGAGCTCCTGAGCAGGCGGAGGTCAAGAGGCTATCTCAGAAGGCGGACGTGTACAGCTTCGGAGTCTTGCTTCTGGAGGTTCTAACGGGGAGAGCTCCATCCCAGTACCCTTCTCCCGCCCATCCCCGTGTGGAAGAGGAGGAGGACGCGGTGGACCTTCCGAAATGGGTGAGGTCTGTGGTGAAGGAGGAGTGGACCGGGGAGGTGTTTGATCAAGAACTTCTGAGGTACAAGAACATTGAGGAGGAGCTTGTTGCAATGCTCCATGTGGGATTGGCTTGTGTGGTGCCTCAGCCTGAGAAGAGGCCTACAATGTCCGAAGTAGCTAAGATGATTGAGGACATCCGAGTCGAGAGATCCCCACTCGGAGAAGACTATGATGATTCCCGCAATTCGCTTTCGCCTTCTCTCGTCACCACTGAAGACGGGCTGGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0042546 GO:0044085 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

683

Amino Acids

74.42

Weight (kDa)

7.19

Isoelectric Point (pI)

50.88

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 198 1.3e-08 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 376 - 647 6.5e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 376 - 647 7.9e-33 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36570
fragaria_vesca FvH4_6g17500
malus_domestica MD12G1097300.v1.1 MD14G1094000.v1.1
prunus_persica Prupe.7G120500_v2.0.a1
pyrus_communis pycom12g09760 pycom14g09180
rosa_chinensis RchiOBHm_Chr3g0471741
rosa_laevigata RLG00000024137
rosa_multiflora Rmu_co8460341.1_g000001 Rmu_sc0013806.1_g000002
rosa_roxburghii Rroxscaffold_6G00409590
rosa_rugosa Rorug03G0119500
rosa_samantha Rh3AG170800 Rh3BG196500 Rh3CG187200 Rh3DG178600
rosa_wichuraiana Rw0G009060 Rw3G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 221, 1958
AccB1I GGYRCC 3 cut(s) 326, 751, 1897
AccBSI CCGCTC 3 cut(s) 147, 957, 972
AccII CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
AccIII TCCGGA 1 cut(s) 392
AclWI GGATC 3 cut(s) 716, 1427, 1962
AcoI YGGCCR 2 cut(s) 870, 942
AcsI RAATTY 2 cut(s) 399, 1395
AcuI CTGAAG 1 cut(s) 104
AcyI GRCGYC 2 cut(s) 1118, 1536
AfaI GTAC 9 cut(s) 608, 1007, 1042, 1160, 1247, 1488, 1664, 1718, 1841
AfiI CCNNNNNNNGG 5 cut(s) 183, 184, 423, 694, 1153
AflIII ACRYGT 1 cut(s) 1659
AgsI TTSAA 1 cut(s) 634
AjiI CACGTC 1 cut(s) 1660
AjnI CCWGG 3 cut(s) 95, 497, 1454
AjuI GAANNNNNNNTTGG 2 cut(s) 102, 134
Alw21I GWGCWC 4 cut(s) 585, 1187, 1618, 1708
Alw26I GTCTC 1 cut(s) 912
AlwI GGATC 3 cut(s) 716, 1427, 1962
Ama87I CYCGRG 1 cut(s) 931
Aor13HI TCCGGA 1 cut(s) 392
ApaI GGGCCC 2 cut(s) 103, 260
ApeKI GCWGC 3 cut(s) 616, 978, 1111
ApoI RAATTY 2 cut(s) 399, 1395
Asp700I GAANNNNTTC 1 cut(s) 638
AspA2I CCTAGG 1 cut(s) 1147
AspLEI GCGC 2 cut(s) 839, 914
AsuC2I CCSGG 9 cut(s) 180, 254, 464, 661, 834, 946, 1389, 1580, 1810
AsuHPI GGTGA 8 cut(s) 478, 583, 700, 1279, 1297, 1792, 1804, 2017
AvaI CYCGRG 1 cut(s) 931
AvaII GGWCC 5 cut(s) 156, 467, 1385, 1765, 1806
AvrII CCTAGG 1 cut(s) 1147
BaeGI GKGCMC 2 cut(s) 103, 260
BalI TGGCCA 1 cut(s) 872
BanI GGYRCC 3 cut(s) 326, 751, 1897
BanII GRGCYC 8 cut(s) 103, 260, 585, 689, 791, 833, 1618, 1708
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
BauI CACGAG 1 cut(s) 1443
BbsI GAAGAC 3 cut(s) 1500, 1989, 2040
Bbv12I GWGCWC 4 cut(s) 585, 1187, 1618, 1708
BbvCI CCTCAGC 1 cut(s) 1902
BbvI GCAGC 3 cut(s) 603, 990, 1098
BccI CCATC 3 cut(s) 934, 1717, 1740
BceAI ACGGC 2 cut(s) 151, 739
BcgI CGANNNNNNTGC 4 cut(s) 673, 707, 1361, 1395
BciT130I CCWGG 3 cut(s) 97, 499, 1456
BciVI GTATCC 1 cut(s) 1601
BclI TGATCA 1 cut(s) 1822
BcnI CCSGG 9 cut(s) 180, 254, 464, 661, 834, 946, 1389, 1580, 1810
BcoDI GTCTC 1 cut(s) 912
BfaI CTAG 1 cut(s) 1148
BfuI GTATCC 1 cut(s) 1601
BglI GCCNNNNNGGC 1 cut(s) 324
BglII AGATCT 1 cut(s) 411
BisI GCNGC 8 cut(s) 617, 735, 925, 928, 979, 1112, 1202, 1440
BlnI CCTAGG 1 cut(s) 1147
BlpI GCTNAGC 1 cut(s) 827
BlsI GCNGC 8 cut(s) 618, 736, 926, 929, 980, 1113, 1203, 1441
Bme18I GGWCC 5 cut(s) 156, 467, 1385, 1765, 1806
BmeT110I CYCGRG 1 cut(s) 931
BmgBI CACGTC 1 cut(s) 1660
BmrI ACTGGG 1 cut(s) 1708
BmsI GCATC 3 cut(s) 157, 188, 1282
BmuI ACTGGG 1 cut(s) 1708
BpiI GAAGAC 3 cut(s) 1500, 1989, 2040
BplI GAGNNNNNCTC 4 cut(s) 401, 433, 1630, 1662
BpmI CTGGAG 4 cut(s) 210, 503, 1121, 1706
Bpu10I CCTNAGC 2 cut(s) 1620, 1902
Bpu1102I GCTNAGC 1 cut(s) 827
BpuEI CTTGAG 3 cut(s) 344, 560, 1190
BpuMI CCSGG 9 cut(s) 180, 254, 464, 661, 834, 946, 1389, 1580, 1810
BsaBI GATNNNNATC 1 cut(s) 854
BsaHI GRCGYC 2 cut(s) 1118, 1536
BsaI GGTCTC 1 cut(s) 912
BsaWI WCCGGW 2 cut(s) 392, 1080
Bsc4I CCNNNNNNNGG 5 cut(s) 183, 184, 423, 694, 1153
Bse118I RCCGGY 3 cut(s) 158, 694, 710
Bse1I ACTGG 2 cut(s) 158, 1714
Bse3DI GCAATG 3 cut(s) 1504, 1590, 1875
Bse8I GATNNNNATC 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 392
BseBI CCWGG 3 cut(s) 97, 499, 1456
BseGI GGATG 6 cut(s) 740, 1258, 1273, 1709, 1732, 1953
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 5 cut(s) 183, 184, 423, 694, 1153
BseMI GCAATG 3 cut(s) 1504, 1590, 1875
BseMII CTCAG 8 cut(s) 791, 818, 1283, 1611, 1661, 1826, 1899, 1916
BseNI ACTGG 2 cut(s) 158, 1714
BseSI GKGCMC 2 cut(s) 103, 260
BseXI GCAGC 3 cut(s) 603, 990, 1098
BseYI CCCAGC 1 cut(s) 183
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
Bsh1285I CGRYCG 2 cut(s) 852, 1081
BshNI GGYRCC 3 cut(s) 326, 751, 1897
BsiEI CGRYCG 2 cut(s) 852, 1081
BsiHKAI GWGCWC 4 cut(s) 585, 1187, 1618, 1708
BsiHKCI CYCGRG 1 cut(s) 931
BslFI GGGAC 4 cut(s) 480, 530, 1043, 1165
BslI CCNNNNNNNGG 5 cut(s) 183, 184, 423, 694, 1153
BsmAI GTCTC 1 cut(s) 912
BsmFI GGGAC 4 cut(s) 480, 530, 1043, 1165
Bso31I GGTCTC 1 cut(s) 912
BsoBI CYCGRG 1 cut(s) 931
Bsp120I GGGCCC 2 cut(s) 99, 256
Bsp1286I GDGCHC 9 cut(s) 103, 260, 585, 689, 791, 833, 1187, 1618, 1708
Bsp13I TCCGGA 1 cut(s) 392
Bsp1407I TGTACA 2 cut(s) 1158, 1662
Bsp143I GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
Bsp1720I GCTNAGC 1 cut(s) 827
Bsp19I CCATGG 1 cut(s) 1188
Bsp68I TCGCGA 2 cut(s) 720, 860
BspCNI CTCAG 8 cut(s) 790, 819, 1284, 1612, 1660, 1827, 1900, 1915
BspEI TCCGGA 1 cut(s) 392
BspFNI CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
BspPI GGATC 3 cut(s) 716, 1427, 1962
BspT107I GGYRCC 3 cut(s) 326, 751, 1897
BspTNI GGTCTC 1 cut(s) 912
BsrBI CCGCTC 3 cut(s) 147, 957, 972
BsrDI GCAATG 3 cut(s) 1504, 1590, 1875
BsrFI RCCGGY 3 cut(s) 158, 694, 710
BsrGI TGTACA 2 cut(s) 1158, 1662
BsrI ACTGG 2 cut(s) 158, 1714
BssAI RCCGGY 3 cut(s) 158, 694, 710
BssMI GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
BssNI GRCGYC 2 cut(s) 1118, 1536
BssSI CACGAG 1 cut(s) 1443
BssT1I CCWWGG 3 cut(s) 1147, 1188, 1311
Bst2BI CACGAG 1 cut(s) 1443
Bst2UI CCWGG 3 cut(s) 97, 499, 1456
Bst4CI ACNGT 5 cut(s) 331, 751, 1034, 1040, 1156
Bst6I CTCTTC 4 cut(s) 236, 1311, 1740, 1907
BstACI GRCGYC 2 cut(s) 1118, 1536
BstAPI GCANNNNNTGC 1 cut(s) 845
BstAUI TGTACA 2 cut(s) 1158, 1662
BstDEI CTNAG 9 cut(s) 777, 827, 1292, 1620, 1647, 1835, 1902, 1908, 1938
BstDSI CCRYGG 3 cut(s) 133, 922, 1188
BstF5I GGATG 6 cut(s) 740, 1258, 1273, 1709, 1732, 1953
BstFNI CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
BstHHI GCGC 2 cut(s) 839, 914
BstKTI GATC 8 cut(s) 414, 724, 852, 858, 994, 1422, 1825, 1970
BstMAI GTCTC 1 cut(s) 912
BstMBI GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
BstMCI CGRYCG 2 cut(s) 852, 1081
BstNI CCWGG 3 cut(s) 97, 499, 1456
BstNSI RCATGY 1 cut(s) 174
BstSLI GKGCMC 2 cut(s) 103, 260
BstUI CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
BstV1I GCAGC 3 cut(s) 603, 990, 1098
BstV2I GAAGAC 3 cut(s) 1500, 1989, 2040
BstX2I RGATCY 2 cut(s) 411, 1967
BstXI CCANNNNNNTGG 2 cut(s) 880, 1407
BstYI RGATCY 2 cut(s) 411, 1967
BsuI GTATCC 1 cut(s) 1601
BtgI CCRYGG 3 cut(s) 133, 922, 1188
BtgZI GCGATG 1 cut(s) 660
BtrI CACGTC 1 cut(s) 1660
BtsCI GGATG 6 cut(s) 740, 1258, 1273, 1709, 1732, 1953
BtsIMutI CAGTG 2 cut(s) 1039, 2028
BtuMI TCGCGA 2 cut(s) 720, 860
CfoI GCGC 2 cut(s) 839, 914
Cfr10I RCCGGY 3 cut(s) 158, 694, 710
Cfr42I CCGCGG 1 cut(s) 925
CseI GACGC 4 cut(s) 1107, 1220, 1525, 1766
Csp6I GTAC 9 cut(s) 607, 1006, 1041, 1159, 1246, 1487, 1663, 1717, 1840
CviAII CATG 8 cut(s) 171, 874, 1189, 1249, 1373, 1463, 1493, 1877
CviQI GTAC 9 cut(s) 607, 1006, 1041, 1159, 1246, 1487, 1663, 1717, 1840
DdeI CTNAG 9 cut(s) 777, 827, 1292, 1620, 1647, 1835, 1902, 1908, 1938
DpnI GATC 8 cut(s) 413, 723, 851, 857, 993, 1421, 1824, 1969
DpnII GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
DrdI GACNNNNNNGTC 2 cut(s) 221, 1958
DseDI GACNNNNNNGTC 2 cut(s) 221, 1958
EaeI YGGCCR 2 cut(s) 870, 942
Eam1104I CTCTTC 4 cut(s) 236, 1311, 1740, 1907
EarI CTCTTC 4 cut(s) 236, 1311, 1740, 1907
Ecl136II GAGCTC 3 cut(s) 583, 1616, 1706
Eco130I CCWWGG 3 cut(s) 1147, 1188, 1311
Eco147I AGGCCT 1 cut(s) 1918
Eco24I GRGCYC 8 cut(s) 103, 260, 585, 689, 791, 833, 1618, 1708
Eco31I GGTCTC 1 cut(s) 912
Eco47I GGWCC 5 cut(s) 156, 467, 1385, 1765, 1806
Eco53kI GAGCTC 3 cut(s) 583, 1616, 1706
Eco57I CTGAAG 1 cut(s) 104
Eco88I CYCGRG 1 cut(s) 931
EcoICRI GAGCTC 3 cut(s) 583, 1616, 1706
EcoO109I RGGNCCY 3 cut(s) 100, 256, 467
EcoRI GAATTC 1 cut(s) 1395
EcoRII CCWGG 3 cut(s) 95, 497, 1454
EcoT14I CCWWGG 3 cut(s) 1147, 1188, 1311
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 8 cut(s) 103, 260, 585, 689, 791, 833, 1618, 1708
ErhI CCWWGG 3 cut(s) 1147, 1188, 1311
FaeI CATG 8 cut(s) 174, 877, 1192, 1252, 1376, 1466, 1496, 1880
FalI AAGNNNNNCTT 2 cut(s) 1308, 1340
FaqI GGGAC 4 cut(s) 480, 530, 1043, 1165
FatI CATG 8 cut(s) 170, 873, 1188, 1248, 1372, 1462, 1492, 1876
FauI CCCGC 7 cut(s) 671, 965, 1010, 1019, 1107, 1735, 2006
FbaI TGATCA 1 cut(s) 1822
Fnu4HI GCNGC 8 cut(s) 617, 735, 925, 928, 979, 1112, 1202, 1440
FokI GGATG 6 cut(s) 727, 1260, 1265, 1696, 1719, 1940
FriOI GRGCYC 8 cut(s) 103, 260, 585, 689, 791, 833, 1618, 1708
Fsp4HI GCNGC 8 cut(s) 617, 735, 925, 928, 979, 1112, 1202, 1440
FspBI CTAG 1 cut(s) 1148
GlaI GCGC 2 cut(s) 838, 913
GluI GCNGC 8 cut(s) 617, 735, 925, 928, 979, 1112, 1202, 1440
GsaI CCCAGC 1 cut(s) 187
GsuI CTGGAG 4 cut(s) 210, 503, 1121, 1706
HgaI GACGC 4 cut(s) 1107, 1220, 1525, 1766
HhaI GCGC 2 cut(s) 839, 914
Hin1I GRCGYC 2 cut(s) 1118, 1536
Hin1II CATG 8 cut(s) 174, 877, 1192, 1252, 1376, 1466, 1496, 1880
Hin6I GCGC 2 cut(s) 837, 912
HinP1I GCGC 2 cut(s) 837, 912
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 3 cut(s) 652, 1322, 1354
HinfI GANTC 8 cut(s) 192, 266, 429, 1378, 1459, 1674, 1959, 1994
HphI GGTGA 8 cut(s) 478, 583, 700, 1279, 1297, 1792, 1804, 2017
Hpy166II GTNNAC 7 cut(s) 346, 1006, 1159, 1585, 1663, 1765, 1806
Hpy8I GTNNAC 7 cut(s) 346, 1006, 1159, 1585, 1663, 1765, 1806
Hpy99I CGWCG 5 cut(s) 409, 787, 1123, 1181, 1541
HpyCH4III ACNGT 5 cut(s) 331, 751, 1034, 1040, 1156
HpyCH4IV ACGT 1 cut(s) 1659
HpyCH4V TGCA 8 cut(s) 170, 174, 351, 1360, 1372, 1545, 1595, 1868
HpyF3I CTNAG 9 cut(s) 777, 827, 1292, 1620, 1647, 1835, 1902, 1908, 1938
HpySE526I ACGT 1 cut(s) 1659
Hsp92I GRCGYC 2 cut(s) 1118, 1536
Hsp92II CATG 8 cut(s) 174, 877, 1192, 1252, 1376, 1466, 1496, 1880
HspAI GCGC 2 cut(s) 837, 912
KflI GGGWCCC 1 cut(s) 467
Kpn2I TCCGGA 1 cut(s) 392
KroI GCCGGC 1 cut(s) 694
KroNI GCCGGC 1 cut(s) 696
Ksp22I TGATCA 1 cut(s) 1822
KspI CCGCGG 1 cut(s) 925
Kzo9I GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
Lsp1109I GCAGC 3 cut(s) 603, 990, 1098
LweI GCATC 3 cut(s) 157, 188, 1282
MaeI CTAG 1 cut(s) 1148
MaeII ACGT 1 cut(s) 1659
MaeIII GTNAC 2 cut(s) 1034, 2023
MalI GATC 8 cut(s) 413, 723, 851, 857, 993, 1421, 1824, 1969
MbiI CCGCTC 3 cut(s) 147, 957, 972
MboI GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
MfeI CAATTG 1 cut(s) 770
MflI RGATCY 2 cut(s) 411, 1967
MhlI GDGCHC 9 cut(s) 103, 260, 585, 689, 791, 833, 1187, 1618, 1708
MlsI TGGCCA 1 cut(s) 872
MluCI AATT 8 cut(s) 110, 399, 586, 602, 770, 862, 1395, 2002
MluNI TGGCCA 1 cut(s) 872
MlyI GAGTC 5 cut(s) 201, 260, 423, 1683, 1968
MmeI TCCRAC 4 cut(s) 174, 387, 474, 1575
Mox20I TGGCCA 1 cut(s) 872
Mph1103I ATGCAT 1 cut(s) 172
MroI TCCGGA 1 cut(s) 392
MroNI GCCGGC 1 cut(s) 694
MroXI GAANNNNTTC 1 cut(s) 638
MscI TGGCCA 1 cut(s) 872
MslI CAYNNNNRTG 6 cut(s) 878, 1193, 1253, 1278, 1497, 1737
Msp20I TGGCCA 1 cut(s) 872
MspA1I CMGCKG 2 cut(s) 924, 1017
MunI CAATTG 1 cut(s) 770
MvaI CCWGG 3 cut(s) 97, 499, 1456
MvnI CGCG 8 cut(s) 219, 720, 860, 914, 924, 1010, 1214, 1760
NaeI GCCGGC 1 cut(s) 696
NciI CCSGG 9 cut(s) 180, 254, 464, 661, 834, 946, 1389, 1580, 1810
NcoI CCATGG 1 cut(s) 1188
NdeII GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
NgoMIV GCCGGC 1 cut(s) 694
NlaIII CATG 8 cut(s) 174, 877, 1192, 1252, 1376, 1466, 1496, 1880
NmeAIII GCCGAG 2 cut(s) 708, 710
NmuCI GTSAC 2 cut(s) 1034, 2023
NruI TCGCGA 2 cut(s) 720, 860
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PaeI GCATGC 1 cut(s) 174
PaeR7I CTCGAG 1 cut(s) 931
PceI AGGCCT 1 cut(s) 1918
PcsI WCGNNNNNNNCGW 4 cut(s) 279, 381, 849, 864
PdiI GCCGGC 1 cut(s) 696
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 3 cut(s) 1378, 1459, 1994
PfoI TCCNGGA 1 cut(s) 659
PkrI GCNGC 8 cut(s) 618, 736, 926, 929, 980, 1113, 1203, 1441
Ple19I CGATCG 1 cut(s) 852
PleI GAGTC 5 cut(s) 200, 260, 423, 1682, 1967
PpsI GAGTC 5 cut(s) 200, 260, 423, 1682, 1967
PpuMI RGGWCCY 1 cut(s) 467
Psp124BI GAGCTC 3 cut(s) 585, 1618, 1708
Psp5II RGGWCCY 1 cut(s) 467
Psp6I CCWGG 3 cut(s) 95, 497, 1454
PspFI CCCAGC 1 cut(s) 183
PspGI CCWGG 3 cut(s) 95, 497, 1454
PspOMI GGGCCC 2 cut(s) 99, 256
PspPPI RGGWCCY 1 cut(s) 467
PspXI VCTCGAGB 1 cut(s) 931
PsuI RGATCY 2 cut(s) 411, 1967
PvuI CGATCG 1 cut(s) 852
RruI TCGCGA 2 cut(s) 720, 860
RsaI GTAC 9 cut(s) 608, 1007, 1042, 1160, 1247, 1488, 1664, 1718, 1841
RsaNI GTAC 9 cut(s) 607, 1006, 1041, 1159, 1246, 1487, 1663, 1717, 1840
RseI CAYNNNNRTG 6 cut(s) 878, 1193, 1253, 1278, 1497, 1737
SacI GAGCTC 3 cut(s) 585, 1618, 1708
SacII CCGCGG 1 cut(s) 925
SatI GCNGC 8 cut(s) 617, 735, 925, 928, 979, 1112, 1202, 1440
Sau3AI GATC 8 cut(s) 411, 721, 849, 855, 991, 1419, 1822, 1967
SchI GAGTC 5 cut(s) 201, 260, 423, 1683, 1968
SduI GDGCHC 9 cut(s) 103, 260, 585, 689, 791, 833, 1187, 1618, 1708
SfaNI GCATC 3 cut(s) 157, 188, 1282
Sfr274I CTCGAG 1 cut(s) 931
Sfr303I CCGCGG 1 cut(s) 925
SgrBI CCGCGG 1 cut(s) 925
SinI GGWCC 5 cut(s) 156, 467, 1385, 1765, 1806
SlaI CTCGAG 1 cut(s) 931
SmiMI CAYNNNNRTG 6 cut(s) 878, 1193, 1253, 1278, 1497, 1737
SmlI CTYRAG 4 cut(s) 359, 575, 931, 1205
SmoI CTYRAG 4 cut(s) 359, 575, 931, 1205
SphI GCATGC 1 cut(s) 174
Sse9I AATT 8 cut(s) 110, 399, 586, 602, 770, 862, 1395, 2002
SseBI AGGCCT 1 cut(s) 1918
SspMI CTAG 1 cut(s) 1148
SstI GAGCTC 3 cut(s) 585, 1618, 1708
StuI AGGCCT 1 cut(s) 1918
StyI CCWWGG 3 cut(s) 1147, 1188, 1311
TaaI ACNGT 5 cut(s) 331, 751, 1034, 1040, 1156
TaiI ACGT 1 cut(s) 1662
TaqI TCGA 8 cut(s) 105, 264, 282, 300, 932, 938, 1077, 1962
TaqII GACCGA 1 cut(s) 140
TasI AATT 8 cut(s) 110, 399, 586, 602, 770, 862, 1395, 2002
TatI WGTACW 3 cut(s) 1158, 1245, 1662
TauI GCSGC 5 cut(s) 737, 927, 930, 1204, 1442
TfiI GAWTC 3 cut(s) 1378, 1459, 1994
TscAI CASTG 2 cut(s) 1039, 2035
TseFI GTSAC 2 cut(s) 1034, 2023
TseI GCWGC 3 cut(s) 616, 978, 1111
Tsp45I GTSAC 2 cut(s) 1034, 2023
TspDTI ATGAA 7 cut(s) 17, 663, 681, 693, 729, 883, 1451
TspGWI ACGGA 5 cut(s) 393, 624, 829, 999, 1067
TspRI CASTG 2 cut(s) 1039, 2035
VpaK11BI GGWCC 5 cut(s) 156, 467, 1385, 1765, 1806
XapI RAATTY 2 cut(s) 399, 1395
XceI RCATGY 1 cut(s) 174
XcmI CCANNNNNNNNNTGG 1 cut(s) 1597
XhoI CTCGAG 1 cut(s) 931
XmaJI CCTAGG 1 cut(s) 1147
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 1 cut(s) 1148
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.