Rh3BG196500

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0012382
Physical Location & Seq
Reverse (-)
17397155 .. 17401898
4744 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG196500.1

Sequence Viewer

Length: 2037 bp
ATGAAGAAGAAGGCTTATCCTTTCGCCTTAGCCTTCCTACTCCTACTCACCTCGGCTAAACTCTCACTCTCCTCTTCGCCGCCGAACGACACGTCAGCTCTCACCGACTTCCGCCTCAAAACCGACACCCACGGCTACCTCCGGTCCAACTGGACCGGACCCGACCCTTGCGCCGCTGCATGGGTCGGAGTCCGATGCTCCAATAACAACCTCCGAGTCGTCGGTCTCTCCCTTCCTTCCCTCAACCTGCGCGGCCCGCTCGACTCTCTGGCTCAGCTGGACCAACTTCGCCTTCTTGACCTCCACAACAACCGTCTCAACGGCACCGTTTTGCCCTTAGCCAACTGCACCAACCTCAAGCTGCTCTACCTCGCCGGGAACGACCTCTCCGGCGAGATCCCGCAGGAATTCGCCTCCCTCCGGCGGCTTCTCCGTCTCGACCTCTCCGACAACAACATCCGCGGCGCCGTCCCCGACGGCCTCTCCCGCCTGAGCCGGCTCCTCACGCTCCGGCTCCAGAACAACCTCCTCACCGGCAAGATCCCCGACCTCTCCGGCTCCCTCTCCGATCTGAAGGAGCTTAACCTGACCAACAACGAGCTCTATGGAAAATTGCCGGAGGGTTTGCTCAGAAAATTCGGCGAGGAAAGCTTTTCCGGCAACGAAGGGCTCTGCGGCTCGTCTCCGTTACCGGCTTGCTCCTTCACCGGAGCTAACCCACCCTCGGCGGCTTCGACCGAAACGGTGCCTTCTAACCCCAGCTCGTTGCCGCAGACAAGCATCATAAACGACGAGCCGAGGGTCAAAAACTCGAGAAAGGGATTGAGTCCTGGAGCAATTGTTGCAATTGTGATAGCGAATTGCGTAGCCATTCTCGTTATCATTTCGTTCATTGTGGCTCACTACTGCGCCAGAGACCGAAGCTCGAACTCGATCGGGGGAAGCGAAGCCGGGAAGAGGAAGAGTGGGAGTAGCTATGGAGGTGAAAAGAGGGTGTATGCTAACAATGGGGGTGGTGACAGTGATGGGACTAATGCTACTGACCGGAGTAAGCTGGTGTTTTTCGACAGGAGAAAGCAGTTTGAGCTCGAGGACTTGCTCCGGGCGTCGGCGGAGATGTTGGGGAAAGGGAGCTTGGGGACGGTGTACAAGGCGGTGCTTGATGATGGTTGTACAATGGCGGTGAAGAGGCTCAAGGATGCGAACCCTTGTGCGAGGAAAGAGTTTGAGCAGTACATGGATTTGATTGGGAAGATTAAGCACCCTAATGTGGTGAGGCTTAGTGCTTATTACTATGCTAAGGAGGAGAAGCTTCTTGTGTATGACTATCTGCCTAATGGGAGCTTGCACTCACTTCTTCATGAGAATCGCGGTCCGGGCAGAATTCCTTTGGATTGGACTACAAGGATCAGCTTGGTGTTGGGAGCTGCTCGCGGCCTAGCTCGGATTCATGAAGAGTTTAGCGCTGCAAAAGTACCACATGGGAATGTGAAGTCTTCCAACGTGCTGCTTGACAAGAACGGTGTGGCTTGCATTTCTGACTTCGGACTGTCCCTGCTTCTGAACCCGGTTCACGCCATTGCGAGGTTGGGAGGATACAGAGCTCCTGAACAGGCAGAGACCAAGAGGCTATCGCAGACAGCAGATGTGTACAGCTTCGGGGTCTTGTTGTTAGAGGTTCTCACAGGGAGAGCTCCTTCCCTGTACCCTTCTCCTACTCGCCCACGCATGGATGAGGAGGAGCAGGCAGTGGACCTGCCGAAATGGGTGAGGTCTGTGGTGAAGGAAGAGTGGACTGGAGAGGTGTTTGATCAGGAACTTCTGAGGTACAAGAACATCGAGGAGGAGCTTGTGTCAATGCTTCATGTGGGACTGGCATGCGTGGTGCCTCAGCCAGAAAAGAGGCCTACTATGGTTGAAGTGGCTAAGATGGTTGAGGACATAAGAGTGGAGAGATCTCCTCTTGGGGAAGACTATGATGATTCCCGCAATTCGCTTTCTCCTTCCATTCCCACCACCGAAGATGGGCTAGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0042546 GO:0044085 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

678

Amino Acids

73.99

Weight (kDa)

7.9

Isoelectric Point (pI)

43.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 198 3.9e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 372 - 643 8.8e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 372 - 643 3.2e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36570
fragaria_vesca FvH4_6g17500
malus_domestica MD12G1097300.v1.1 MD14G1094000.v1.1
prunus_persica Prupe.7G120500_v2.0.a1
pyrus_communis pycom12g09760 pycom14g09180
rosa_chinensis RchiOBHm_Chr3g0471741
rosa_laevigata RLG00000024137
rosa_multiflora Rmu_co8460341.1_g000001 Rmu_sc0013806.1_g000002
rosa_roxburghii Rroxscaffold_6G00409590
rosa_rugosa Rorug03G0119500
rosa_samantha Rh3AG170800 Rh3BG196500 Rh3CG187200 Rh3DG178600
rosa_wichuraiana Rw0G009060 Rw3G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 255, 1764
AccB1I GGYRCC 4 cut(s) 323, 464, 745, 1885
AccBSI CCGCTC 1 cut(s) 259
AccII CGCG 4 cut(s) 252, 462, 1371, 1434
AclWI GGATC 3 cut(s) 391, 535, 1415
AcsI RAATTY 3 cut(s) 407, 635, 1383
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 2 cut(s) 465, 1106
AfaI GTAC 7 cut(s) 1148, 1174, 1235, 1476, 1652, 1706, 1829
AfeI AGCGCT 1 cut(s) 1465
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 1 cut(s) 1919
AjiI CACGTC 1 cut(s) 93
AjnI CCWGG 1 cut(s) 829
AjuI GAANNNNNNNTTGG 4 cut(s) 276, 308, 1118, 1150
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
Alw21I GWGCWC 4 cut(s) 603, 1089, 1606, 1696
Alw26I GTCTC 6 cut(s) 230, 320, 440, 687, 909, 1613
AlwI GGATC 3 cut(s) 391, 535, 1415
Ama87I CYCGRG 2 cut(s) 811, 1088
Aor51HI AGCGCT 1 cut(s) 1465
AoxI GGCC 4 cut(s) 253, 478, 1435, 1904
ApeKI GCWGC 5 cut(s) 176, 361, 1427, 1466, 1507
ApoI RAATTY 3 cut(s) 407, 635, 1383
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 5 cut(s) 173, 252, 467, 911, 1466
AspS9I GGNCC 7 cut(s) 144, 153, 158, 254, 280, 1373, 1753
AsuC2I CCSGG 5 cut(s) 376, 952, 1103, 1377, 1568
AsuNHI GCTAGC 1 cut(s) 2029
AvaI CYCGRG 2 cut(s) 811, 1088
AvaII GGWCC 6 cut(s) 144, 153, 158, 280, 1373, 1753
BanI GGYRCC 4 cut(s) 323, 464, 745, 1885
BanII GRGCYC 5 cut(s) 603, 672, 1089, 1606, 1696
BbsI GAAGAC 2 cut(s) 1488, 1977
Bbv12I GWGCWC 4 cut(s) 603, 1089, 1606, 1696
BbvCI CCTCAGC 1 cut(s) 1890
BbvI GCAGC 5 cut(s) 163, 348, 1414, 1453, 1494
BccI CCATC 4 cut(s) 1019, 1160, 1924, 2018
BceAI ACGGC 4 cut(s) 148, 337, 452, 493
BciT130I CCWGG 1 cut(s) 831
BciVI GTATCC 1 cut(s) 1589
BclI TGATCA 1 cut(s) 1810
BcnI CCSGG 5 cut(s) 376, 952, 1103, 1377, 1568
BcoDI GTCTC 6 cut(s) 230, 320, 440, 687, 909, 1613
BfaI CTAG 2 cut(s) 1439, 2030
BfoI RGCGCY 2 cut(s) 468, 1467
BfuAI ACCTGC 2 cut(s) 255, 1764
BfuI GTATCC 1 cut(s) 1589
BglII AGATCT 1 cut(s) 1955
BlpI GCTNAGC 1 cut(s) 273
Bme1390I CCNGG 6 cut(s) 376, 831, 952, 1103, 1377, 1568
Bme18I GGWCC 6 cut(s) 144, 153, 158, 280, 1373, 1753
BmeT110I CYCGRG 2 cut(s) 811, 1088
BmgBI CACGTC 1 cut(s) 93
BmgT120I GGNCC 7 cut(s) 144, 153, 158, 254, 280, 1373, 1753
BmiI GGNNCC 8 cut(s) 160, 325, 466, 500, 515, 559, 747, 1887
BmrFI CCNGG 6 cut(s) 376, 831, 952, 1103, 1377, 1568
BmsI GCATC 3 cut(s) 185, 789, 1189
BmtI GCTAGC 1 cut(s) 2033
BpiI GAAGAC 2 cut(s) 1488, 1977
BplI GAGNNNNNCTC 2 cut(s) 1945, 1977
BpmI CTGGAG 3 cut(s) 500, 852, 1818
Bpu10I CCTNAGC 5 cut(s) 28, 337, 491, 1299, 1890
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 2 cut(s) 341, 1178
BpuMI CCSGG 5 cut(s) 376, 952, 1103, 1377, 1568
BsaHI GRCGYC 2 cut(s) 465, 1106
BsaI GGTCTC 3 cut(s) 230, 909, 1613
BsaJI CCNNGG 5 cut(s) 51, 130, 460, 723, 797
BsaWI WCCGGW 4 cut(s) 141, 155, 707, 1044
BsaXI ACNNNNNCTCC 8 cut(s) 371, 401, 467, 497, 825, 855, 1838, 1868
Bse118I RCCGGY 3 cut(s) 495, 533, 691
Bse1I ACTGG 3 cut(s) 155, 1801, 1878
Bse3DI GCAATG 1 cut(s) 1578
BseBI CCWGG 1 cut(s) 831
BseDI CCNNGG 5 cut(s) 51, 130, 460, 723, 797
BseGI GGATG 3 cut(s) 456, 1204, 1738
BseMI GCAATG 1 cut(s) 1578
BseMII CTCAG 5 cut(s) 287, 482, 643, 1814, 1904
BseNI ACTGG 3 cut(s) 155, 1801, 1878
BseRI GAGGAG 9 cut(s) 61, 491, 518, 1319, 1751, 1754, 1856, 1859, 1950
BseXI GCAGC 5 cut(s) 163, 348, 1414, 1453, 1494
BseYI CCCAGC 1 cut(s) 758
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 4 cut(s) 252, 462, 1371, 1434
Bsh1285I CGRYCG 2 cut(s) 738, 936
BshFI GGCC 4 cut(s) 255, 480, 1437, 1906
BshNI GGYRCC 4 cut(s) 323, 464, 745, 1885
BsiEI CGRYCG 2 cut(s) 738, 936
BsiHKAI GWGCWC 4 cut(s) 603, 1089, 1606, 1696
BsiHKCI CYCGRG 2 cut(s) 811, 1088
BslFI GGGAC 5 cut(s) 455, 1042, 1153, 1537, 1884
BsmAI GTCTC 6 cut(s) 230, 320, 440, 687, 909, 1613
BsmBI CGTCTC 3 cut(s) 320, 440, 687
BsmFI GGGAC 5 cut(s) 455, 1042, 1153, 1537, 1884
BsnI GGCC 4 cut(s) 255, 480, 1437, 1906
Bso31I GGTCTC 3 cut(s) 230, 909, 1613
BsoBI CYCGRG 2 cut(s) 811, 1088
Bsp1286I GDGCHC 5 cut(s) 603, 672, 1089, 1606, 1696
Bsp1407I TGTACA 3 cut(s) 1146, 1172, 1650
Bsp143I GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 4 cut(s) 255, 480, 1437, 1906
BspCNI CTCAG 5 cut(s) 286, 483, 642, 1815, 1903
BspFNI CGCG 4 cut(s) 252, 462, 1371, 1434
BspHI TCATGA 2 cut(s) 1360, 1450
BspLI GGNNCC 8 cut(s) 160, 325, 466, 500, 515, 559, 747, 1887
BspMI ACCTGC 2 cut(s) 255, 1764
BspOI GCTAGC 1 cut(s) 2033
BspPI GGATC 3 cut(s) 391, 535, 1415
BspT107I GGYRCC 4 cut(s) 323, 464, 745, 1885
BspTNI GGTCTC 3 cut(s) 230, 909, 1613
BsrBI CCGCTC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 1578
BsrFI RCCGGY 3 cut(s) 495, 533, 691
BsrGI TGTACA 3 cut(s) 1146, 1172, 1650
BsrI ACTGG 3 cut(s) 155, 1801, 1878
BssAI RCCGGY 3 cut(s) 495, 533, 691
BssECI CCNNGG 5 cut(s) 51, 130, 460, 723, 797
BssMI GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
BssNI GRCGYC 2 cut(s) 465, 1106
Bst2UI CCWGG 1 cut(s) 831
Bst4CI ACNGT 7 cut(s) 314, 328, 745, 1022, 1144, 1523, 1551
Bst6I CTCTTC 6 cut(s) 79, 950, 956, 1181, 1449, 1782
BstACI GRCGYC 2 cut(s) 465, 1106
BstAPI GCANNNNNTGC 1 cut(s) 842
BstAUI TGTACA 3 cut(s) 1146, 1172, 1650
BstC8I GCNNGC 9 cut(s) 257, 497, 697, 1346, 1432, 1531, 1746, 1879, 2031
BstDSI CCRYGG 2 cut(s) 130, 460
BstF5I GGATG 3 cut(s) 456, 1204, 1738
BstFNI CGCG 4 cut(s) 252, 462, 1371, 1434
BstH2I RGCGCY 2 cut(s) 468, 1467
BstHHI GCGC 5 cut(s) 173, 252, 467, 911, 1466
BstKTI GATC 7 cut(s) 399, 543, 571, 936, 1410, 1813, 1958
BstMAI GTCTC 6 cut(s) 230, 320, 440, 687, 909, 1613
BstMBI GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
BstMCI CGRYCG 2 cut(s) 738, 936
BstMWI GCNNNNNNNGC 8 cut(s) 256, 486, 505, 648, 657, 842, 1084, 1377
BstNI CCWGG 1 cut(s) 831
BstNSI RCATGY 1 cut(s) 1881
BstSCI CCNGG 6 cut(s) 374, 829, 950, 1101, 1375, 1566
BstUI CGCG 4 cut(s) 252, 462, 1371, 1434
BstV1I GCAGC 5 cut(s) 163, 348, 1414, 1453, 1494
BstV2I GAAGAC 2 cut(s) 1488, 1977
BstX2I RGATCY 3 cut(s) 396, 540, 1955
BstYI RGATCY 3 cut(s) 396, 540, 1955
BsuI GTATCC 1 cut(s) 1589
BsuRI GGCC 4 cut(s) 255, 480, 1437, 1906
BtgI CCRYGG 2 cut(s) 130, 460
BtrI CACGTC 1 cut(s) 93
BtsCI GGATG 3 cut(s) 456, 1204, 1738
BtsI GCAGTG 1 cut(s) 1755
BtsIMutI CAGTG 2 cut(s) 1027, 1755
BveI ACCTGC 2 cut(s) 255, 1764
Cac8I GCNNGC 9 cut(s) 257, 497, 697, 1346, 1432, 1531, 1746, 1879, 2031
CciI TCATGA 2 cut(s) 1360, 1450
CfoI GCGC 5 cut(s) 173, 252, 467, 911, 1466
Cfr10I RCCGGY 3 cut(s) 495, 533, 691
Cfr13I GGNCC 7 cut(s) 144, 153, 158, 254, 280, 1373, 1753
Cfr42I CCGCGG 1 cut(s) 463
CpoI CGGWCCG 1 cut(s) 1373
CseI GACGC 1 cut(s) 1095
Csp6I GTAC 7 cut(s) 1147, 1173, 1234, 1475, 1651, 1705, 1828
CspCI CAANNNNNGTGG 2 cut(s) 994, 1029
CspI CGGWCCG 1 cut(s) 1373
CviAII CATG 8 cut(s) 180, 1237, 1361, 1451, 1481, 1729, 1865, 1878
CviQI GTAC 7 cut(s) 1147, 1173, 1234, 1475, 1651, 1705, 1828
DinI GGCGCC 1 cut(s) 466
DpnI GATC 7 cut(s) 398, 542, 570, 935, 1409, 1812, 1957
DpnII GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
Eam1104I CTCTTC 6 cut(s) 79, 950, 956, 1181, 1449, 1782
EarI CTCTTC 6 cut(s) 79, 950, 956, 1181, 1449, 1782
EciI GGCGGA 2 cut(s) 101, 1127
Ecl136II GAGCTC 4 cut(s) 601, 1087, 1604, 1694
Eco147I AGGCCT 1 cut(s) 1906
Eco24I GRGCYC 5 cut(s) 603, 672, 1089, 1606, 1696
Eco31I GGTCTC 3 cut(s) 230, 909, 1613
Eco47I GGWCC 6 cut(s) 144, 153, 158, 280, 1373, 1753
Eco47III AGCGCT 1 cut(s) 1465
Eco53kI GAGCTC 4 cut(s) 601, 1087, 1604, 1694
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 2 cut(s) 811, 1088
EcoICRI GAGCTC 4 cut(s) 601, 1087, 1604, 1694
EcoRI GAATTC 2 cut(s) 407, 1383
EcoRII CCWGG 1 cut(s) 829
EcoT38I GRGCYC 5 cut(s) 603, 672, 1089, 1606, 1696
EgeI GGCGCC 1 cut(s) 466
EheI GGCGCC 1 cut(s) 466
Esp3I CGTCTC 3 cut(s) 320, 440, 687
FaeI CATG 8 cut(s) 183, 1240, 1364, 1454, 1484, 1732, 1868, 1881
FaqI GGGAC 5 cut(s) 455, 1042, 1153, 1537, 1884
FatI CATG 8 cut(s) 179, 1236, 1360, 1450, 1480, 1728, 1864, 1877
FauI CCCGC 4 cut(s) 264, 408, 494, 1994
FbaI TGATCA 1 cut(s) 1810
FokI GGATG 3 cut(s) 443, 1211, 1745
FriOI GRGCYC 5 cut(s) 603, 672, 1089, 1606, 1696
FspBI CTAG 2 cut(s) 1439, 2030
GlaI GCGC 5 cut(s) 172, 251, 466, 910, 1465
GsaI CCCAGC 1 cut(s) 762
GsuI CTGGAG 3 cut(s) 500, 852, 1818
HaeII RGCGCY 2 cut(s) 468, 1467
HaeIII GGCC 4 cut(s) 255, 480, 1437, 1906
HgaI GACGC 1 cut(s) 1095
HhaI GCGC 5 cut(s) 173, 252, 467, 911, 1466
Hin1I GRCGYC 2 cut(s) 465, 1106
Hin1II CATG 8 cut(s) 183, 1240, 1364, 1454, 1484, 1732, 1868, 1881
Hin6I GCGC 5 cut(s) 171, 250, 465, 909, 1464
HinP1I GCGC 5 cut(s) 171, 250, 465, 909, 1464
HindIII AAGCTT 2 cut(s) 649, 1310
HinfI GANTC 7 cut(s) 189, 216, 263, 826, 1366, 1447, 1982
Hpy166II GTNNAC 5 cut(s) 1147, 1573, 1651, 1753, 1794
Hpy188III TCNNGA 8 cut(s) 296, 437, 517, 813, 1361, 1451, 1607, 1814
Hpy8I GTNNAC 5 cut(s) 1147, 1573, 1651, 1753, 1794
Hpy99I CGWCG 4 cut(s) 224, 479, 794, 1111
HpyCH4III ACNGT 7 cut(s) 314, 328, 745, 1022, 1144, 1523, 1551
HpyCH4IV ACGT 2 cut(s) 92, 1503
HpyCH4V TGCA 6 cut(s) 179, 348, 845, 1348, 1469, 1533
HpyF10VI GCNNNNNNNGC 8 cut(s) 256, 486, 505, 648, 657, 842, 1084, 1377
HpySE526I ACGT 2 cut(s) 92, 1503
Hsp92I GRCGYC 2 cut(s) 465, 1106
Hsp92II CATG 8 cut(s) 183, 1240, 1364, 1454, 1484, 1732, 1868, 1881
HspAI GCGC 5 cut(s) 171, 250, 465, 909, 1464
KasI GGCGCC 1 cut(s) 464
KroI GCCGGC 1 cut(s) 495
KroNI GCCGGC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 1810
KspI CCGCGG 1 cut(s) 463
Kzo9I GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
Lsp1109I GCAGC 5 cut(s) 163, 348, 1414, 1453, 1494
LweI GCATC 3 cut(s) 185, 789, 1189
MaeI CTAG 2 cut(s) 1439, 2030
MaeII ACGT 2 cut(s) 92, 1503
MaeIII GTNAC 2 cut(s) 687, 1016
MalI GATC 7 cut(s) 398, 542, 570, 935, 1409, 1812, 1957
MbiI CCGCTC 1 cut(s) 259
MboI GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
MfeI CAATTG 2 cut(s) 837, 846
MflI RGATCY 3 cut(s) 396, 540, 1955
MhlI GDGCHC 5 cut(s) 603, 672, 1089, 1606, 1696
MluCI AATT 8 cut(s) 407, 611, 635, 837, 846, 859, 1383, 1990
Mly113I GGCGCC 1 cut(s) 465
MlyI GAGTC 4 cut(s) 198, 225, 257, 835
MmeI TCCRAC 4 cut(s) 166, 171, 471, 1524
MroNI GCCGGC 1 cut(s) 495
MseI TTAA 2 cut(s) 582, 1257
MslI CAYNNNNRTG 3 cut(s) 1266, 1485, 1946
MspA1I CMGCKG 3 cut(s) 176, 277, 462
MspR9I CCNGG 6 cut(s) 376, 831, 952, 1103, 1377, 1568
MunI CAATTG 2 cut(s) 837, 846
MvaI CCWGG 1 cut(s) 831
MvnI CGCG 4 cut(s) 252, 462, 1371, 1434
MwoI GCNNNNNNNGC 8 cut(s) 256, 486, 505, 648, 657, 842, 1084, 1377
NaeI GCCGGC 1 cut(s) 497
NarI GGCGCC 1 cut(s) 465
NciI CCSGG 5 cut(s) 376, 952, 1103, 1377, 1568
NdeII GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
NgoMIV GCCGGC 1 cut(s) 495
NheI GCTAGC 1 cut(s) 2029
NlaIII CATG 8 cut(s) 183, 1240, 1364, 1454, 1484, 1732, 1868, 1881
NlaIV GGNNCC 8 cut(s) 160, 325, 466, 500, 515, 559, 747, 1887
NmeAIII GCCGAG 3 cut(s) 32, 704, 822
NmuCI GTSAC 1 cut(s) 1016
NspI RCATGY 1 cut(s) 1881
PaeI GCATGC 1 cut(s) 1881
PaeR7I CTCGAG 2 cut(s) 811, 1088
PagI TCATGA 2 cut(s) 1360, 1450
PceI AGGCCT 1 cut(s) 1906
PcsI WCGNNNNNNNCGW 4 cut(s) 378, 444, 731, 942
PdiI GCCGGC 1 cut(s) 497
PfeI GAWTC 3 cut(s) 1366, 1447, 1982
PfoI TCCNGGA 1 cut(s) 829
Ple19I CGATCG 1 cut(s) 936
PleI GAGTC 4 cut(s) 197, 224, 257, 834
PluTI GGCGCC 1 cut(s) 468
PpsI GAGTC 4 cut(s) 197, 224, 257, 834
Psp124BI GAGCTC 4 cut(s) 603, 1089, 1606, 1696
Psp6I CCWGG 1 cut(s) 829
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 1 cut(s) 829
PspN4I GGNNCC 8 cut(s) 160, 325, 466, 500, 515, 559, 747, 1887
PspPI GGNCC 7 cut(s) 144, 153, 158, 254, 280, 1373, 1753
PspXI VCTCGAGB 1 cut(s) 1088
PsuI RGATCY 3 cut(s) 396, 540, 1955
PvuI CGATCG 1 cut(s) 936
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 7 cut(s) 1148, 1174, 1235, 1476, 1652, 1706, 1829
RsaNI GTAC 7 cut(s) 1147, 1173, 1234, 1475, 1651, 1705, 1828
RseI CAYNNNNRTG 3 cut(s) 1266, 1485, 1946
Rsr2I CGGWCCG 1 cut(s) 1373
RsrII CGGWCCG 1 cut(s) 1373
SacI GAGCTC 4 cut(s) 603, 1089, 1606, 1696
SacII CCGCGG 1 cut(s) 463
SaqAI TTAA 2 cut(s) 582, 1257
Sau3AI GATC 7 cut(s) 396, 540, 568, 933, 1407, 1810, 1955
Sau96I GGNCC 7 cut(s) 144, 153, 158, 254, 280, 1373, 1753
SchI GAGTC 4 cut(s) 198, 225, 257, 835
ScrFI CCNGG 6 cut(s) 376, 831, 952, 1103, 1377, 1568
SduI GDGCHC 5 cut(s) 603, 672, 1089, 1606, 1696
SfaNI GCATC 3 cut(s) 185, 789, 1189
SfoI GGCGCC 1 cut(s) 466
Sfr274I CTCGAG 2 cut(s) 811, 1088
Sfr303I CCGCGG 1 cut(s) 463
SgrBI CCGCGG 1 cut(s) 463
SinI GGWCC 6 cut(s) 144, 153, 158, 280, 1373, 1753
SlaI CTCGAG 2 cut(s) 811, 1088
SmiMI CAYNNNNRTG 3 cut(s) 1266, 1485, 1946
SmlI CTYRAG 4 cut(s) 356, 811, 1088, 1193
SmoI CTYRAG 4 cut(s) 356, 811, 1088, 1193
SphI GCATGC 1 cut(s) 1881
Sse9I AATT 8 cut(s) 407, 611, 635, 837, 846, 859, 1383, 1990
SseBI AGGCCT 1 cut(s) 1906
SspDI GGCGCC 1 cut(s) 464
SspMI CTAG 2 cut(s) 1439, 2030
SstI GAGCTC 4 cut(s) 603, 1089, 1606, 1696
StuI AGGCCT 1 cut(s) 1906
StyD4I CCNGG 6 cut(s) 374, 829, 950, 1101, 1375, 1566
TaaI ACNGT 7 cut(s) 314, 328, 745, 1022, 1144, 1523, 1551
TaiI ACGT 2 cut(s) 95, 1506
TaqI TCGA 9 cut(s) 261, 438, 734, 812, 926, 932, 1065, 1089, 1839
TaqII GACCGA 3 cut(s) 212, 752, 933
TasI AATT 8 cut(s) 407, 611, 635, 837, 846, 859, 1383, 1990
TatI WGTACW 4 cut(s) 1146, 1172, 1233, 1650
TauI GCSGC 9 cut(s) 82, 176, 255, 427, 465, 678, 731, 772, 1437
TfiI GAWTC 3 cut(s) 1366, 1447, 1982
Tru1I TTAA 2 cut(s) 582, 1257
Tru9I TTAA 2 cut(s) 582, 1257
TscAI CASTG 2 cut(s) 1027, 1755
TseFI GTSAC 1 cut(s) 1016
TseI GCWGC 5 cut(s) 176, 361, 1427, 1466, 1507
Tsp45I GTSAC 1 cut(s) 1016
TspDTI ATGAA 6 cut(s) 17, 880, 1349, 1439, 1467, 1853
TspGWI ACGGA 2 cut(s) 422, 675
TspRI CASTG 2 cut(s) 1027, 1755
VpaK11BI GGWCC 6 cut(s) 144, 153, 158, 280, 1373, 1753
XapI RAATTY 3 cut(s) 407, 635, 1383
XceI RCATGY 1 cut(s) 1881
XcmI CCANNNNNNNNNTGG 1 cut(s) 1585
XhoI CTCGAG 2 cut(s) 811, 1088
XspI CTAG 2 cut(s) 1439, 2030
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.