RLG00000024137

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0012382
Physical Location & Seq
Forward (+)
32359347 .. 32361467
2121 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024137

Sequence Viewer

Length: 2034 bp
ATGAAGAAGAAGGCTTATCCTATCGCCTTAGCCTTCCTACTCCTACTCACCTCGGCTAAACTCTCAATCTCCTCTTCGCCGCCGAACGACACGTCAGCTCTCACCGACTTCCGCCTCAAAACCGACACCCACGGCTACCTCCGCTCCAACTGGACCGGACCCGACCCTTGCGCCGCTGCATGGGTCGGAGTCCGATGCTCCAATAACAACCTCCGAGTCGTCGGTCTCTCCCTTCCTTCCCTCAACCTGCGCGGCCCGCTCGACTCTCTGGCTCAGCTGGACCAACTCCGCCTTCTTGACCTCCACAACAACCGCCTCAACGGCACCGTTTTGCCCTTAGCTAACTGCACCAACCTTAAGCTGCTCTACCTCGCCGGGAACGACCTCTCAGGCGAGATCCCGCCTGAATTCGCCTCTCTCAGGAGGCTTCTCCGTCTCGACCTCTCCGACAACAACATCCGCGGCGCGGTCCCCGACGGCATCTCCCGCCTGAGCAGGCTCCTCACGCTCCGGCTCCAGAACAACCTCCTCACCGGCAAGATCCCCGACCTCTCCGGCTCCCTCTCCGACCTGAAGGAGCTTAACCTGACCAACAACGAGCTCTATGGAAAATTGCCGGAGGGTTTGCTCAGAAAATTCGGCGAGGAAAGCTTTTCCGGCAACGAAGGGCTCTGCGGCTCGTCTCCGTTACCGGCTTGCTCCTTCACCGGAGCTAACCCACCGTCGGCGGCTTCGACAGAAACGGTGCCGTCTAACCCCAGCTCGTTGCCGCAAACAAGCATCATAAACGAGCCGAGGGTCAAAAACTCGAGAAAGGGATTGAGTCCTGGAGCAATAGTAGCAATTGTGATAGCGAATTGCGTAGCCATTCTCGTTATCATTTCGTTCATTGTGGCTCACTACTGCGCCAGAGACCGAAGCTCGAACTCGATAGGGGGAAGCGAAGCCGGGAAGAGGAAGAGTGGGAGTAGCTATGGAGGTGAAAAGAGGGTGTATGCTAACAATGGGGGTGGTGACAGTGATGGGACTAATGCTACTGACCGGAGTAAGCTGGTGTTTTTCGACCGGAGGAAGCAGTTTGAGCTGGAGGACTTGCTCCGGGCCTCGGCGGAGATGTTGGGGAAAGGGAGCTTGGGGACGGTGTACAAGGCGGTGCTTGATGATGGTTGTACAATGGCGGTGAAGAGGCTCAAGGATGCGAACCCTTGTGCGAGGAAAGAGTTTGAGCAGTATATGGATTTGATTGGGAAGATTAAGCACCCTAATGTGGTGAGGCTTAGTGCTTATTACTATGCTAAGGAGGAGAAGCTTCTTGTCTATGATTATCTGCCTAATGGGAGCTTGCACTCACTTCTTCATGAGAATCGCGGTCCGGGCAGAATTCCTTTGGATTGGACTACAAGGATCAGCTTGGTGTTGGGAGCTGCCCGAGGACTAGCTCGGATTCATGAAGAGTTTAGCGCTGCAAAAGTACCCCATGGGAATGTGAAGTCTTCCAATGTGCTGCTTGACAAGAATGGTGTGGCTTGCATTTCTGACTTCGGACTGTCCCTGCTTCTGAACCCGGTTCACGCCATTGCCAGGTTGGGAGGATACAGAGCTCCTGAGCAGGCAGAGACCAAGAGGCTATCGCAGACAGCAGACGTGTACAGCTTCGGGGTCTTGTTGTTGGAGGTTCTCACAGGGAGAGCTCCTTCCCTGTACCCTTCTCCTACTCGCCCACGCATGGATGAGGAGGAGCAGGCGGTGGACCTGCCGAAATGGGTGAGGTCTGTGGTGAAGGAAGAGTGGACTGGAGAGGTGTTTGATCAGGAACTTCTGAGGTACAAGAACATCGAGGAGGAGCTTGTGTCAATGCTCCATGTGGGATTGGCATGCGTGGTGCCTCAGCCAGAAAAGAGGCCTACTATGGTTGAAGTGGCTAAGATGGTTGAGGACATAAGAGTGGAGAGATCTCCTCTAGGGGAAGACTATGATGATTCCCGCAATTCGCTTTCTCCTTCCATTCCCACCACCGAAGATGGGCTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0042546 GO:0044085 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

678

Amino Acids

73.81

Weight (kDa)

8.16

Isoelectric Point (pI)

43.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 198 1.1e-07 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 371 - 642 8.7e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 371 - 642 3.1e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36570
fragaria_vesca FvH4_6g17500
malus_domestica MD12G1097300.v1.1 MD14G1094000.v1.1
prunus_persica Prupe.7G120500_v2.0.a1
pyrus_communis pycom12g09760 pycom14g09180
rosa_chinensis RchiOBHm_Chr3g0471741
rosa_laevigata RLG00000024137
rosa_multiflora Rmu_co8460341.1_g000001 Rmu_sc0013806.1_g000002
rosa_roxburghii Rroxscaffold_6G00409590
rosa_rugosa Rorug03G0119500
rosa_samantha Rh3AG170800 Rh3BG196500 Rh3CG187200 Rh3DG178600
rosa_wichuraiana Rw0G009060 Rw3G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 255, 1761
AccB1I GGYRCC 3 cut(s) 323, 745, 1882
AccBSI CCGCTC 2 cut(s) 144, 259
AccII CGCG 4 cut(s) 252, 462, 467, 1368
AclWI GGATC 3 cut(s) 391, 535, 1412
AcsI RAATTY 3 cut(s) 407, 635, 1380
AcuI CTGAAG 1 cut(s) 593
AfaI GTAC 6 cut(s) 1145, 1171, 1473, 1649, 1703, 1826
AfeI AGCGCT 1 cut(s) 1462
AflII CTTAAG 1 cut(s) 356
AflIII ACRYGT 2 cut(s) 90, 1644
AgsI TTSAA 1 cut(s) 1916
AjiI CACGTC 2 cut(s) 93, 1645
AjnI CCWGG 2 cut(s) 826, 1580
AjuI GAANNNNNNNTTGG 4 cut(s) 276, 308, 1115, 1147
Alw21I GWGCWC 3 cut(s) 603, 1603, 1693
Alw26I GTCTC 5 cut(s) 230, 440, 687, 906, 1610
AlwI GGATC 3 cut(s) 391, 535, 1412
Ama87I CYCGRG 2 cut(s) 808, 1428
Aor51HI AGCGCT 1 cut(s) 1462
AoxI GGCC 3 cut(s) 253, 1101, 1901
ApeKI GCWGC 5 cut(s) 176, 361, 1424, 1463, 1504
ApoI RAATTY 3 cut(s) 407, 635, 1380
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 5 cut(s) 173, 252, 467, 908, 1463
AspS9I GGNCC 8 cut(s) 153, 158, 254, 280, 469, 1101, 1370, 1750
AsuC2I CCSGG 5 cut(s) 376, 949, 1100, 1374, 1565
AsuNHI GCTAGC 1 cut(s) 2026
AvaI CYCGRG 2 cut(s) 808, 1428
AvaII GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
BanI GGYRCC 3 cut(s) 323, 745, 1882
BanII GRGCYC 4 cut(s) 603, 672, 1603, 1693
BbsI GAAGAC 2 cut(s) 1485, 1974
Bbv12I GWGCWC 3 cut(s) 603, 1603, 1693
BbvCI CCTCAGC 1 cut(s) 1887
BbvI GCAGC 5 cut(s) 163, 348, 1411, 1450, 1491
BccI CCATC 4 cut(s) 1016, 1157, 1921, 2015
BceAI ACGGC 4 cut(s) 148, 337, 493, 733
BciT130I CCWGG 2 cut(s) 828, 1582
BciVI GTATCC 1 cut(s) 1586
BclI TGATCA 1 cut(s) 1807
BcnI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1565
BcoDI GTCTC 5 cut(s) 230, 440, 687, 906, 1610
BfaI CTAG 3 cut(s) 1436, 1961, 2027
BfoI RGCGCY 1 cut(s) 1464
BfrI CTTAAG 1 cut(s) 356
BfuAI ACCTGC 2 cut(s) 255, 1761
BfuI GTATCC 1 cut(s) 1586
BglI GCCNNNNNGGC 1 cut(s) 321
BglII AGATCT 1 cut(s) 1952
BlpI GCTNAGC 1 cut(s) 273
Bme1390I CCNGG 7 cut(s) 376, 828, 949, 1100, 1374, 1565, 1582
Bme18I GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
BmeT110I CYCGRG 2 cut(s) 808, 1428
BmgBI CACGTC 2 cut(s) 93, 1645
BmgT120I GGNCC 8 cut(s) 153, 158, 254, 280, 469, 1101, 1370, 1750
BmiI GGNNCC 8 cut(s) 160, 325, 471, 500, 515, 559, 747, 1884
BmrFI CCNGG 7 cut(s) 376, 828, 949, 1100, 1374, 1565, 1582
BmsI GCATC 4 cut(s) 185, 489, 789, 1186
BmtI GCTAGC 1 cut(s) 2030
BpiI GAAGAC 2 cut(s) 1485, 1974
BplI GAGNNNNNCTC 2 cut(s) 1942, 1974
BpmI CTGGAG 4 cut(s) 500, 849, 1106, 1815
Bpu10I CCTNAGC 6 cut(s) 28, 337, 491, 1296, 1605, 1887
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 1 cut(s) 1175
BpuMI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1565
BsaI GGTCTC 3 cut(s) 230, 906, 1610
BsaJI CCNNGG 7 cut(s) 51, 130, 460, 794, 1104, 1429, 1477
BsaWI WCCGGW 4 cut(s) 155, 707, 1041, 1065
BsaXI ACNNNNNCTCC 8 cut(s) 128, 158, 467, 497, 822, 852, 1835, 1865
Bse118I RCCGGY 2 cut(s) 533, 691
Bse1I ACTGG 2 cut(s) 155, 1798
Bse3DI GCAATG 1 cut(s) 1575
BseBI CCWGG 2 cut(s) 828, 1582
BseDI CCNNGG 7 cut(s) 51, 130, 460, 794, 1104, 1429, 1477
BseGI GGATG 3 cut(s) 456, 1201, 1735
BseMI GCAATG 1 cut(s) 1575
BseMII CTCAG 8 cut(s) 287, 402, 433, 482, 643, 1596, 1811, 1901
BseNI ACTGG 2 cut(s) 155, 1798
BseRI GAGGAG 9 cut(s) 61, 491, 518, 1316, 1748, 1751, 1853, 1856, 1947
BseXI GCAGC 5 cut(s) 163, 348, 1411, 1450, 1491
BseYI CCCAGC 1 cut(s) 758
BsgI GTGCAG 1 cut(s) 331
Bsh1236I CGCG 4 cut(s) 252, 462, 467, 1368
Bsh1285I CGRYCG 1 cut(s) 1066
BshFI GGCC 3 cut(s) 255, 1103, 1903
BshNI GGYRCC 3 cut(s) 323, 745, 1882
BsiEI CGRYCG 1 cut(s) 1066
BsiHKAI GWGCWC 3 cut(s) 603, 1603, 1693
BsiHKCI CYCGRG 2 cut(s) 808, 1428
BslFI GGGAC 4 cut(s) 455, 1039, 1150, 1534
BsmAI GTCTC 5 cut(s) 230, 440, 687, 906, 1610
BsmBI CGTCTC 2 cut(s) 440, 687
BsmFI GGGAC 4 cut(s) 455, 1039, 1150, 1534
BsnI GGCC 3 cut(s) 255, 1103, 1903
Bso31I GGTCTC 3 cut(s) 230, 906, 1610
BsoBI CYCGRG 2 cut(s) 808, 1428
Bsp1286I GDGCHC 4 cut(s) 603, 672, 1603, 1693
Bsp1407I TGTACA 3 cut(s) 1143, 1169, 1647
Bsp143I GATC 5 cut(s) 396, 540, 1404, 1807, 1952
Bsp1720I GCTNAGC 1 cut(s) 273
Bsp19I CCATGG 1 cut(s) 1477
BspANI GGCC 3 cut(s) 255, 1103, 1903
BspCNI CTCAG 8 cut(s) 286, 401, 432, 483, 642, 1597, 1812, 1900
BspFNI CGCG 4 cut(s) 252, 462, 467, 1368
BspHI TCATGA 2 cut(s) 1357, 1447
BspLI GGNNCC 8 cut(s) 160, 325, 471, 500, 515, 559, 747, 1884
BspMI ACCTGC 2 cut(s) 255, 1761
BspOI GCTAGC 1 cut(s) 2030
BspPI GGATC 3 cut(s) 391, 535, 1412
BspT107I GGYRCC 3 cut(s) 323, 745, 1882
BspTI CTTAAG 1 cut(s) 356
BspTNI GGTCTC 3 cut(s) 230, 906, 1610
BsrBI CCGCTC 2 cut(s) 144, 259
BsrDI GCAATG 1 cut(s) 1575
BsrFI RCCGGY 2 cut(s) 533, 691
BsrGI TGTACA 3 cut(s) 1143, 1169, 1647
BsrI ACTGG 2 cut(s) 155, 1798
BssAI RCCGGY 2 cut(s) 533, 691
BssECI CCNNGG 7 cut(s) 51, 130, 460, 794, 1104, 1429, 1477
BssMI GATC 5 cut(s) 396, 540, 1404, 1807, 1952
BssT1I CCWWGG 1 cut(s) 1477
Bst2UI CCWGG 2 cut(s) 828, 1582
Bst4CI ACNGT 6 cut(s) 328, 723, 745, 1019, 1141, 1548
Bst6I CTCTTC 6 cut(s) 79, 947, 953, 1178, 1446, 1779
BstAFI CTTAAG 1 cut(s) 356
BstAUI TGTACA 3 cut(s) 1143, 1169, 1647
BstC8I GCNNGC 9 cut(s) 257, 497, 697, 1343, 1528, 1611, 1743, 1876, 2028
BstDSI CCRYGG 3 cut(s) 130, 460, 1477
BstENI CCTNNNNNAGG 1 cut(s) 418
BstF5I GGATG 3 cut(s) 456, 1201, 1735
BstFNI CGCG 4 cut(s) 252, 462, 467, 1368
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 5 cut(s) 173, 252, 467, 908, 1463
BstKTI GATC 5 cut(s) 399, 543, 1407, 1810, 1955
BstMAI GTCTC 5 cut(s) 230, 440, 687, 906, 1610
BstMBI GATC 5 cut(s) 396, 540, 1404, 1807, 1952
BstMCI CGRYCG 1 cut(s) 1066
BstNI CCWGG 2 cut(s) 828, 1582
BstNSI RCATGY 1 cut(s) 1878
BstSCI CCNGG 7 cut(s) 374, 826, 947, 1098, 1372, 1563, 1580
BstUI CGCG 4 cut(s) 252, 462, 467, 1368
BstV1I GCAGC 5 cut(s) 163, 348, 1411, 1450, 1491
BstV2I GAAGAC 2 cut(s) 1485, 1974
BstX2I RGATCY 3 cut(s) 396, 540, 1952
BstYI RGATCY 3 cut(s) 396, 540, 1952
BsuI GTATCC 1 cut(s) 1586
BsuRI GGCC 3 cut(s) 255, 1103, 1903
BtgI CCRYGG 3 cut(s) 130, 460, 1477
BtrI CACGTC 2 cut(s) 93, 1645
BtsCI GGATG 3 cut(s) 456, 1201, 1735
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 2 cut(s) 255, 1761
Cac8I GCNNGC 9 cut(s) 257, 497, 697, 1343, 1528, 1611, 1743, 1876, 2028
CciI TCATGA 2 cut(s) 1357, 1447
CfoI GCGC 5 cut(s) 173, 252, 467, 908, 1463
Cfr10I RCCGGY 2 cut(s) 533, 691
Cfr13I GGNCC 8 cut(s) 153, 158, 254, 280, 469, 1101, 1370, 1750
Cfr42I CCGCGG 1 cut(s) 463
CpoI CGGWCCG 1 cut(s) 1370
Csp6I GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
CspI CGGWCCG 1 cut(s) 1370
CviAII CATG 7 cut(s) 180, 1358, 1448, 1478, 1726, 1862, 1875
CviQI GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
DpnI GATC 5 cut(s) 398, 542, 1406, 1809, 1954
DpnII GATC 5 cut(s) 396, 540, 1404, 1807, 1952
Eam1104I CTCTTC 6 cut(s) 79, 947, 953, 1178, 1446, 1779
EarI CTCTTC 6 cut(s) 79, 947, 953, 1178, 1446, 1779
EciI GGCGGA 3 cut(s) 101, 278, 1124
Ecl136II GAGCTC 3 cut(s) 601, 1601, 1691
Eco130I CCWWGG 1 cut(s) 1477
Eco147I AGGCCT 1 cut(s) 1903
Eco24I GRGCYC 4 cut(s) 603, 672, 1603, 1693
Eco31I GGTCTC 3 cut(s) 230, 906, 1610
Eco47I GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
Eco47III AGCGCT 1 cut(s) 1462
Eco53kI GAGCTC 3 cut(s) 601, 1601, 1691
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 2 cut(s) 808, 1428
EcoICRI GAGCTC 3 cut(s) 601, 1601, 1691
EcoNI CCTNNNNNAGG 1 cut(s) 418
EcoRI GAATTC 2 cut(s) 407, 1380
EcoRII CCWGG 2 cut(s) 826, 1580
EcoT14I CCWWGG 1 cut(s) 1477
EcoT38I GRGCYC 4 cut(s) 603, 672, 1603, 1693
ErhI CCWWGG 1 cut(s) 1477
Esp3I CGTCTC 2 cut(s) 440, 687
FaeI CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
FaqI GGGAC 4 cut(s) 455, 1039, 1150, 1534
FatI CATG 7 cut(s) 179, 1357, 1447, 1477, 1725, 1861, 1874
FauI CCCGC 4 cut(s) 264, 408, 494, 1991
FbaI TGATCA 1 cut(s) 1807
FokI GGATG 3 cut(s) 443, 1208, 1742
FriOI GRGCYC 4 cut(s) 603, 672, 1603, 1693
FspBI CTAG 3 cut(s) 1436, 1961, 2027
GlaI GCGC 5 cut(s) 172, 251, 466, 907, 1462
GsaI CCCAGC 1 cut(s) 762
GsuI CTGGAG 4 cut(s) 500, 849, 1106, 1815
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 3 cut(s) 255, 1103, 1903
HhaI GCGC 5 cut(s) 173, 252, 467, 908, 1463
Hin1II CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
Hin6I GCGC 5 cut(s) 171, 250, 465, 906, 1461
HinP1I GCGC 5 cut(s) 171, 250, 465, 906, 1461
HindIII AAGCTT 2 cut(s) 649, 1307
HinfI GANTC 7 cut(s) 189, 216, 263, 823, 1363, 1444, 1979
Hpy166II GTNNAC 5 cut(s) 1144, 1570, 1648, 1750, 1791
Hpy188III TCNNGA 9 cut(s) 296, 421, 437, 517, 810, 1358, 1448, 1604, 1811
Hpy8I GTNNAC 5 cut(s) 1144, 1570, 1648, 1750, 1791
Hpy99I CGWCG 3 cut(s) 224, 479, 727
HpyCH4III ACNGT 6 cut(s) 328, 723, 745, 1019, 1141, 1548
HpyCH4IV ACGT 2 cut(s) 92, 1644
HpyCH4V TGCA 5 cut(s) 179, 348, 1345, 1466, 1530
HpySE526I ACGT 2 cut(s) 92, 1644
Hsp92II CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
HspAI GCGC 5 cut(s) 171, 250, 465, 906, 1461
Ksp22I TGATCA 1 cut(s) 1807
KspI CCGCGG 1 cut(s) 463
Kzo9I GATC 5 cut(s) 396, 540, 1404, 1807, 1952
Lsp1109I GCAGC 5 cut(s) 163, 348, 1411, 1450, 1491
LweI GCATC 4 cut(s) 185, 489, 789, 1186
MaeI CTAG 3 cut(s) 1436, 1961, 2027
MaeII ACGT 2 cut(s) 92, 1644
MaeIII GTNAC 2 cut(s) 687, 1013
MalI GATC 5 cut(s) 398, 542, 1406, 1809, 1954
MbiI CCGCTC 2 cut(s) 144, 259
MboI GATC 5 cut(s) 396, 540, 1404, 1807, 1952
MfeI CAATTG 1 cut(s) 843
MflI RGATCY 3 cut(s) 396, 540, 1952
MhlI GDGCHC 4 cut(s) 603, 672, 1603, 1693
MluCI AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
MlyI GAGTC 4 cut(s) 198, 225, 257, 832
MmeI TCCRAC 5 cut(s) 166, 171, 471, 591, 1650
MseI TTAA 3 cut(s) 357, 582, 1254
MslI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
MspA1I CMGCKG 3 cut(s) 176, 277, 462
MspCI CTTAAG 1 cut(s) 356
MspR9I CCNGG 7 cut(s) 376, 828, 949, 1100, 1374, 1565, 1582
MunI CAATTG 1 cut(s) 843
MvaI CCWGG 2 cut(s) 828, 1582
MvnI CGCG 4 cut(s) 252, 462, 467, 1368
NciI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1565
NcoI CCATGG 1 cut(s) 1477
NdeII GATC 5 cut(s) 396, 540, 1404, 1807, 1952
NheI GCTAGC 1 cut(s) 2026
NlaIII CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
NlaIV GGNNCC 8 cut(s) 160, 325, 471, 500, 515, 559, 747, 1884
NmeAIII GCCGAG 3 cut(s) 32, 819, 1085
NmuCI GTSAC 1 cut(s) 1013
NspI RCATGY 1 cut(s) 1878
PaeI GCATGC 1 cut(s) 1878
PaeR7I CTCGAG 1 cut(s) 808
PagI TCATGA 2 cut(s) 1357, 1447
PceI AGGCCT 1 cut(s) 1903
PcsI WCGNNNNNNNCGW 3 cut(s) 378, 444, 731
PfeI GAWTC 3 cut(s) 1363, 1444, 1979
PfoI TCCNGGA 1 cut(s) 826
PleI GAGTC 4 cut(s) 197, 224, 257, 831
PpsI GAGTC 4 cut(s) 197, 224, 257, 831
Psp124BI GAGCTC 3 cut(s) 603, 1603, 1693
Psp6I CCWGG 2 cut(s) 826, 1580
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 2 cut(s) 826, 1580
PspN4I GGNNCC 8 cut(s) 160, 325, 471, 500, 515, 559, 747, 1884
PspPI GGNCC 8 cut(s) 153, 158, 254, 280, 469, 1101, 1370, 1750
PsuI RGATCY 3 cut(s) 396, 540, 1952
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 6 cut(s) 1145, 1171, 1473, 1649, 1703, 1826
RsaNI GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
RseI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
Rsr2I CGGWCCG 1 cut(s) 1370
RsrII CGGWCCG 1 cut(s) 1370
SacI GAGCTC 3 cut(s) 603, 1603, 1693
SacII CCGCGG 1 cut(s) 463
SaqAI TTAA 3 cut(s) 357, 582, 1254
Sau3AI GATC 5 cut(s) 396, 540, 1404, 1807, 1952
Sau96I GGNCC 8 cut(s) 153, 158, 254, 280, 469, 1101, 1370, 1750
SchI GAGTC 4 cut(s) 198, 225, 257, 832
ScrFI CCNGG 7 cut(s) 376, 828, 949, 1100, 1374, 1565, 1582
SduI GDGCHC 4 cut(s) 603, 672, 1603, 1693
SfaNI GCATC 4 cut(s) 185, 489, 789, 1186
Sfr274I CTCGAG 1 cut(s) 808
Sfr303I CCGCGG 1 cut(s) 463
SgrBI CCGCGG 1 cut(s) 463
SinI GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
SlaI CTCGAG 1 cut(s) 808
SmiMI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
SmlI CTYRAG 3 cut(s) 356, 808, 1190
SmoI CTYRAG 3 cut(s) 356, 808, 1190
SphI GCATGC 1 cut(s) 1878
Sse9I AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
SseBI AGGCCT 1 cut(s) 1903
SspMI CTAG 3 cut(s) 1436, 1961, 2027
SstI GAGCTC 3 cut(s) 603, 1603, 1693
StuI AGGCCT 1 cut(s) 1903
StyD4I CCNGG 7 cut(s) 374, 826, 947, 1098, 1372, 1563, 1580
StyI CCWWGG 1 cut(s) 1477
TaaI ACNGT 6 cut(s) 328, 723, 745, 1019, 1141, 1548
TaiI ACGT 2 cut(s) 95, 1647
TaqI TCGA 8 cut(s) 261, 438, 734, 809, 923, 929, 1062, 1836
TaqII GACCGA 2 cut(s) 212, 930
TasI AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
TatI WGTACW 3 cut(s) 1143, 1169, 1647
TauI GCSGC 7 cut(s) 82, 176, 255, 465, 678, 731, 772
TfiI GAWTC 3 cut(s) 1363, 1444, 1979
Tru1I TTAA 3 cut(s) 357, 582, 1254
Tru9I TTAA 3 cut(s) 357, 582, 1254
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 1 cut(s) 1013
TseI GCWGC 5 cut(s) 176, 361, 1424, 1463, 1504
Tsp45I GTSAC 1 cut(s) 1013
TspDTI ATGAA 5 cut(s) 17, 877, 1346, 1436, 1464
TspGWI ACGGA 2 cut(s) 422, 675
TspRI CASTG 1 cut(s) 1024
Vha464I CTTAAG 1 cut(s) 356
VpaK11BI GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
XagI CCTNNNNNAGG 1 cut(s) 418
XapI RAATTY 3 cut(s) 407, 635, 1380
XceI RCATGY 1 cut(s) 1878
XcmI CCANNNNNNNNNTGG 1 cut(s) 1582
XhoI CTCGAG 1 cut(s) 808
XspI CTAG 3 cut(s) 1436, 1961, 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.