pycom14g09180

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0012382
Physical Location & Seq
Forward (+)
10799181 .. 10801880
2700 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g09180.1

Sequence Viewer

Length: 1386 bp
ATGAAGAAGAACGAGGCGCTGCCTTTCCTCACTCTCTCTCTTCTTCTCTTCGCCTCCAGCCACCCGTCACTCTCTTCTCCGCCACCTAATGACACCTGGGCCCTCACCCAATTCCGCCTACAGACCGACACCCACGCCTACCTCCGCTCAAACTGGACCGGCTTCGATGCATGCACCCTCTGCTGGACCGGAGTCCGATGCTCCACCAACAAAGCCCGCGTCGTCGCTCTCTCCCTCCCCTCCCTCAACCTCCGGGGCCCACTTGATTCCCTCGCTTCCCTTGACCAGCTCCGCCTCCTCGACCTCCACAACAACCGCCTCAACGGCACCGTTTCTCCTCTCGTCAACTGCACCAAGCTCAAGCTCCTCTACCTCGCCGGCAACGACCTCTCTGGCGAAATTCCATCGGAGATATCCTCCCTCCGGCAACTCCTCCGTCTCGACCTTGCCGACAACAACCTCCGGGGACCGTTTCCGGACAATCTCACCCGCCTCACCCGGCTCCTCACGCTCAGGCTCCAGAACAACGCCATCTCCGGTGTAGTCCCCGACCTCTCCGCCTCCCTTGCCGACCTCAAGGAGCTCAATTTCACTAACAACGAATTGTACGGACGGCTGCCGGAGGTTCTGTTGAAGAAGTTCGGCGACAAAAGCTTTTCCGGGAACGAAGGGCTGTGCGGACTGAGCCCCTTGCCGGCTTGTTCTGTCACCGGCACTAGCGATCCGCCCTCGGCAGCTTCATCCGAAATGGTGCCGTCTAATCCCAGCCAATTGCCTCAGACGACGAGCCCAAACGAGCCGAACAAAAAGCAACCGAGGAAGGGGCTGAGCGCCGGCGTGATCATCGCGATCGTGATCGTGATTTGCGTGGCGATGCTGGTGGTTGTGTCGTTCCTTGTGGCCCACTACTGCGCGAGAGACCGCTGCGGCTCTAGCTTGATGGCGGGGAGCGAGAGCGGGAAGAGAAGGAGCGGAAGCAGCTACGGAGCCGATCAGAAGAGGGTGTACGCCAACAGTGGGGGCGGCGGGGACAGCGACGGGCCAAACGCGACCGACAGAAGCAAACTGGTGTTTTTTGACCCGAGGAAGCAGTTTGAGCTGGAGGATTTGCTGCGGGCGTCGGCGGAGATGTTGGGGAAAGGAAGCCTGGGGACGGTGTACAAGGCGGTGCTGGACGACGGGAGCACCATGGCGGTGAAGCGGCTCAAGGACGCGAACCCGTGCGAGAGAAAGGAGTTTGAGCAGTACATGGATGTGATTGGGAAGGTGAAGCATCCCAATGTGGTGAGGCTGAGTGCTTATTACTATACCAAGGAGGAGAAGCTTCTAGTCTATGATTATCTCCCTAATGGAAGCTTGCACTCACTTCTGCATGAAAGATACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0042546 GO:0044085 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

462

Amino Acids

50.32

Weight (kDa)

8.78

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 198 1.5e-08 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 376 - 459 2.4e-12 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 376 - 459 4.3e-12 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36570
fragaria_vesca FvH4_6g17500
malus_domestica MD12G1097300.v1.1 MD14G1094000.v1.1
prunus_persica Prupe.7G120500_v2.0.a1
pyrus_communis pycom12g09760 pycom14g09180
rosa_chinensis RchiOBHm_Chr3g0471741
rosa_laevigata RLG00000024137
rosa_multiflora Rmu_co8460341.1_g000001 Rmu_sc0013806.1_g000002
rosa_roxburghii Rroxscaffold_6G00409590
rosa_rugosa Rorug03G0119500
rosa_samantha Rh3AG170800 Rh3BG196500 Rh3CG187200 Rh3DG178600
rosa_wichuraiana Rw0G009060 Rw3G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 326, 751
AccBSI CCGCTC 3 cut(s) 147, 957, 972
AccII CGCG 5 cut(s) 219, 848, 914, 1049, 1214
AccIII TCCGGA 1 cut(s) 475
AclWI GGATC 1 cut(s) 716
AcsI RAATTY 1 cut(s) 399
AcyI GRCGYC 1 cut(s) 1118
AfaI GTAC 4 cut(s) 608, 1007, 1160, 1247
AfiI CCNNNNNNNGG 6 cut(s) 183, 423, 694, 821, 1017, 1153
AgsI TTSAA 1 cut(s) 634
AjnI CCWGG 2 cut(s) 95, 1146
Alw21I GWGCWC 2 cut(s) 585, 1187
Alw26I GTCTC 2 cut(s) 443, 912
AlwI GGATC 1 cut(s) 716
Ama87I CYCGRG 1 cut(s) 1081
Aor13HI TCCGGA 1 cut(s) 475
AoxI GGCC 4 cut(s) 99, 256, 900, 1040
ApaI GGGCCC 2 cut(s) 103, 260
ApeKI GCWGC 6 cut(s) 19, 616, 734, 924, 978, 1111
ApoI RAATTY 1 cut(s) 399
ArsI GACNNNNNNTTYG 2 cut(s) 204, 236
Asp700I GAANNNNTTC 1 cut(s) 638
AspLEI GCGC 3 cut(s) 19, 833, 914
AspS9I GGNCC 9 cut(s) 99, 100, 156, 186, 256, 257, 467, 901, 1040
AsuC2I CCSGG 4 cut(s) 254, 464, 499, 661
AsuHPI GGTGA 7 cut(s) 97, 478, 487, 700, 1207, 1279, 1297
AvaI CYCGRG 1 cut(s) 1081
AvaII GGWCC 3 cut(s) 156, 186, 467
BaeGI GKGCMC 2 cut(s) 103, 260
BanI GGYRCC 2 cut(s) 326, 751
BanII GRGCYC 5 cut(s) 103, 260, 585, 689, 791
BarI GAAGNNNNNNTAC 2 cut(s) 989, 1021
Bbv12I GWGCWC 2 cut(s) 585, 1187
BbvI GCAGC 6 cut(s) 6, 603, 746, 911, 990, 1098
BccI CCATC 3 cut(s) 412, 539, 934
BceAI ACGGC 3 cut(s) 340, 629, 739
BciT130I CCWGG 2 cut(s) 97, 1148
BclI TGATCA 1 cut(s) 840
BcnI CCSGG 4 cut(s) 254, 464, 499, 661
BcoDI GTCTC 2 cut(s) 443, 912
BfaI CTAG 3 cut(s) 717, 933, 1328
BfmI CTRYAG 1 cut(s) 119
BfoI RGCGCY 2 cut(s) 20, 834
BglI GCCNNNNNGGC 1 cut(s) 324
BisI GCNGC 9 cut(s) 20, 617, 735, 925, 928, 979, 1024, 1112, 1202
BlpI GCTNAGC 1 cut(s) 827
BlsI GCNGC 9 cut(s) 21, 618, 736, 926, 929, 980, 1025, 1113, 1203
Bme1390I CCNGG 6 cut(s) 97, 254, 464, 499, 661, 1148
Bme18I GGWCC 3 cut(s) 156, 186, 467
BmeT110I CYCGRG 1 cut(s) 1081
BmgT120I GGNCC 9 cut(s) 99, 100, 156, 186, 256, 257, 467, 901, 1040
BmiI GGNNCC 9 cut(s) 101, 257, 258, 328, 468, 503, 518, 753, 988
BmrFI CCNGG 6 cut(s) 97, 254, 464, 499, 661, 1148
BmsI GCATC 4 cut(s) 157, 188, 864, 1282
BoxI GACNNNNGTC 1 cut(s) 191
BplI GAGNNNNNCTC 2 cut(s) 401, 433
BpmI CTGGAG 3 cut(s) 40, 503, 1121
Bpu10I CCTNAGC 1 cut(s) 512
Bpu1102I GCTNAGC 1 cut(s) 827
BpuEI CTTGAG 3 cut(s) 344, 560, 1190
BpuMI CCSGG 4 cut(s) 254, 464, 499, 661
BsaBI GATNNNNATC 1 cut(s) 854
BsaHI GRCGYC 1 cut(s) 1118
BsaI GGTCTC 1 cut(s) 912
BsaJI CCNNGG 9 cut(s) 96, 253, 463, 729, 815, 1082, 1147, 1188, 1311
BsaWI WCCGGW 3 cut(s) 188, 475, 536
BsaXI ACNNNNNCTCC 6 cut(s) 319, 349, 518, 548, 614, 644
Bsc4I CCNNNNNNNGG 6 cut(s) 183, 423, 694, 821, 1017, 1153
Bse118I RCCGGY 5 cut(s) 158, 377, 694, 710, 833
Bse1I ACTGG 2 cut(s) 158, 1071
Bse8I GATNNNNATC 1 cut(s) 854
BseAI TCCGGA 1 cut(s) 475
BseBI CCWGG 2 cut(s) 97, 1148
BseDI CCNNGG 9 cut(s) 96, 253, 463, 729, 815, 1082, 1147, 1188, 1311
BseGI GGATG 3 cut(s) 740, 1258, 1273
BseJI GATNNNNATC 1 cut(s) 854
BseLI CCNNNNNNNGG 6 cut(s) 183, 423, 694, 821, 1017, 1153
BseMII CTCAG 5 cut(s) 526, 674, 791, 818, 1283
BseNI ACTGG 2 cut(s) 158, 1071
BseRI GAGGAG 6 cut(s) 287, 327, 356, 422, 494, 1331
BseSI GKGCMC 2 cut(s) 103, 260
BseXI GCAGC 6 cut(s) 6, 603, 746, 911, 990, 1098
BseYI CCCAGC 1 cut(s) 764
BsgI GTGCAG 1 cut(s) 334
Bsh1236I CGCG 5 cut(s) 219, 848, 914, 1049, 1214
Bsh1285I CGRYCG 2 cut(s) 852, 1053
BshFI GGCC 4 cut(s) 101, 258, 902, 1042
BshNI GGYRCC 2 cut(s) 326, 751
BsiEI CGRYCG 2 cut(s) 852, 1053
BsiHKAI GWGCWC 2 cut(s) 585, 1187
BsiHKCI CYCGRG 1 cut(s) 1081
BslFI GGGAC 4 cut(s) 480, 530, 1043, 1165
BslI CCNNNNNNNGG 6 cut(s) 183, 423, 694, 821, 1017, 1153
BsmAI GTCTC 2 cut(s) 443, 912
BsmBI CGTCTC 1 cut(s) 443
BsmFI GGGAC 4 cut(s) 480, 530, 1043, 1165
BsnI GGCC 4 cut(s) 101, 258, 902, 1042
Bso31I GGTCTC 1 cut(s) 912
BsoBI CYCGRG 1 cut(s) 1081
Bsp120I GGGCCC 2 cut(s) 99, 256
Bsp1286I GDGCHC 6 cut(s) 103, 260, 585, 689, 791, 1187
Bsp13I TCCGGA 1 cut(s) 475
Bsp1407I TGTACA 1 cut(s) 1158
Bsp143I GATC 5 cut(s) 721, 840, 849, 855, 991
Bsp1720I GCTNAGC 1 cut(s) 827
Bsp19I CCATGG 1 cut(s) 1188
Bsp68I TCGCGA 1 cut(s) 848
BspANI GGCC 4 cut(s) 101, 258, 902, 1042
BspCNI CTCAG 5 cut(s) 525, 675, 790, 819, 1284
BspEI TCCGGA 1 cut(s) 475
BspFNI CGCG 5 cut(s) 219, 848, 914, 1049, 1214
BspLI GGNNCC 9 cut(s) 101, 257, 258, 328, 468, 503, 518, 753, 988
BspPI GGATC 1 cut(s) 716
BspT107I GGYRCC 2 cut(s) 326, 751
BspTNI GGTCTC 1 cut(s) 912
BsrBI CCGCTC 3 cut(s) 147, 957, 972
BsrFI RCCGGY 5 cut(s) 158, 377, 694, 710, 833
BsrGI TGTACA 1 cut(s) 1158
BsrI ACTGG 2 cut(s) 158, 1071
BssAI RCCGGY 5 cut(s) 158, 377, 694, 710, 833
BssECI CCNNGG 9 cut(s) 96, 253, 463, 729, 815, 1082, 1147, 1188, 1311
BssMI GATC 5 cut(s) 721, 840, 849, 855, 991
BssNI GRCGYC 1 cut(s) 1118
BssT1I CCWWGG 2 cut(s) 1188, 1311
Bst2UI CCWGG 2 cut(s) 97, 1148
Bst4CI ACNGT 4 cut(s) 331, 471, 1016, 1156
Bst6I CTCTTC 5 cut(s) 45, 53, 79, 956, 992
BstACI GRCGYC 1 cut(s) 1118
BstAPI GCANNNNNTGC 1 cut(s) 180
BstAUI TGTACA 1 cut(s) 1158
BstC8I GCNNGC 7 cut(s) 172, 217, 379, 696, 835, 1116, 1358
BstDEI CTNAG 5 cut(s) 512, 683, 777, 827, 1292
BstDSI CCRYGG 1 cut(s) 1188
BstF5I GGATG 3 cut(s) 740, 1258, 1273
BstFNI CGCG 5 cut(s) 219, 848, 914, 1049, 1214
BstH2I RGCGCY 2 cut(s) 20, 834
BstHHI GCGC 3 cut(s) 19, 833, 914
BstKTI GATC 5 cut(s) 724, 843, 852, 858, 994
BstMAI GTCTC 2 cut(s) 443, 912
BstMBI GATC 5 cut(s) 721, 840, 849, 855, 991
BstMCI CGRYCG 2 cut(s) 852, 1053
BstNI CCWGG 2 cut(s) 97, 1148
BstNSI RCATGY 1 cut(s) 174
BstPAI GACNNNNGTC 1 cut(s) 191
BstSCI CCNGG 6 cut(s) 95, 252, 462, 497, 659, 1146
BstSFI CTRYAG 1 cut(s) 119
BstSLI GKGCMC 2 cut(s) 103, 260
BstUI CGCG 5 cut(s) 219, 848, 914, 1049, 1214
BstV1I GCAGC 6 cut(s) 6, 603, 746, 911, 990, 1098
BsuRI GGCC 4 cut(s) 101, 258, 902, 1042
BtgI CCRYGG 1 cut(s) 1188
BtgZI GCGATG 2 cut(s) 829, 887
BtsCI GGATG 3 cut(s) 740, 1258, 1273
BtsIMutI CAGTG 1 cut(s) 1021
BtuMI TCGCGA 1 cut(s) 848
Cac8I GCNNGC 7 cut(s) 172, 217, 379, 696, 835, 1116, 1358
CfoI GCGC 3 cut(s) 19, 833, 914
Cfr10I RCCGGY 5 cut(s) 158, 377, 694, 710, 833
Cfr13I GGNCC 9 cut(s) 99, 100, 156, 186, 256, 257, 467, 901, 1040
CseI GACGC 3 cut(s) 208, 1107, 1220
Csp6I GTAC 4 cut(s) 607, 1006, 1159, 1246
CviAII CATG 4 cut(s) 171, 1189, 1249, 1373
CviQI GTAC 4 cut(s) 607, 1006, 1159, 1246
DdeI CTNAG 5 cut(s) 512, 683, 777, 827, 1292
DpnI GATC 5 cut(s) 723, 842, 851, 857, 993
DpnII GATC 5 cut(s) 721, 840, 849, 855, 991
Eam1104I CTCTTC 5 cut(s) 45, 53, 79, 956, 992
EarI CTCTTC 5 cut(s) 45, 53, 79, 956, 992
EciI GGCGGA 6 cut(s) 69, 104, 281, 547, 714, 1139
Ecl136II GAGCTC 1 cut(s) 583
Eco130I CCWWGG 2 cut(s) 1188, 1311
Eco24I GRGCYC 5 cut(s) 103, 260, 585, 689, 791
Eco31I GGTCTC 1 cut(s) 912
Eco32I GATATC 1 cut(s) 414
Eco47I GGWCC 3 cut(s) 156, 186, 467
Eco53kI GAGCTC 1 cut(s) 583
Eco88I CYCGRG 1 cut(s) 1081
EcoICRI GAGCTC 1 cut(s) 583
EcoO109I RGGNCCY 2 cut(s) 100, 256
EcoRII CCWGG 2 cut(s) 95, 1146
EcoRV GATATC 1 cut(s) 414
EcoT14I CCWWGG 2 cut(s) 1188, 1311
EcoT22I ATGCAT 1 cut(s) 172
EcoT38I GRGCYC 5 cut(s) 103, 260, 585, 689, 791
ErhI CCWWGG 2 cut(s) 1188, 1311
Esp3I CGTCTC 1 cut(s) 443
FaeI CATG 4 cut(s) 174, 1192, 1252, 1376
FaiI YATR 6 cut(s) 172, 1190, 1250, 1308, 1335, 1374
FaqI GGGAC 4 cut(s) 480, 530, 1043, 1165
FatI CATG 4 cut(s) 170, 1188, 1248, 1372
FauI CCCGC 6 cut(s) 224, 497, 937, 950, 1019, 1107
FbaI TGATCA 1 cut(s) 840
Fnu4HI GCNGC 9 cut(s) 20, 617, 735, 925, 928, 979, 1024, 1112, 1202
FokI GGATG 3 cut(s) 727, 1260, 1265
FriOI GRGCYC 5 cut(s) 103, 260, 585, 689, 791
Fsp4HI GCNGC 9 cut(s) 20, 617, 735, 925, 928, 979, 1024, 1112, 1202
FspBI CTAG 3 cut(s) 717, 933, 1328
GlaI GCGC 3 cut(s) 18, 832, 913
GluI GCNGC 9 cut(s) 20, 617, 735, 925, 928, 979, 1024, 1112, 1202
GsaI CCCAGC 1 cut(s) 768
GsuI CTGGAG 3 cut(s) 40, 503, 1121
HaeII RGCGCY 2 cut(s) 20, 834
HaeIII GGCC 4 cut(s) 101, 258, 902, 1042
HgaI GACGC 3 cut(s) 208, 1107, 1220
HhaI GCGC 3 cut(s) 19, 833, 914
Hin1I GRCGYC 1 cut(s) 1118
Hin1II CATG 4 cut(s) 174, 1192, 1252, 1376
Hin6I GCGC 3 cut(s) 17, 831, 912
HinP1I GCGC 3 cut(s) 17, 831, 912
HincII GTYRAC 1 cut(s) 346
HindII GTYRAC 1 cut(s) 346
HindIII AAGCTT 3 cut(s) 652, 1322, 1354
HinfI GANTC 2 cut(s) 192, 266
HphI GGTGA 7 cut(s) 97, 478, 487, 700, 1207, 1279, 1297
Hpy166II GTNNAC 3 cut(s) 346, 1006, 1159
Hpy188I TCNGA 5 cut(s) 197, 409, 745, 780, 996
Hpy188III TCNNGA 6 cut(s) 440, 476, 520, 847, 853, 859
Hpy8I GTNNAC 3 cut(s) 346, 1006, 1159
Hpy99I CGWCG 6 cut(s) 224, 227, 787, 1040, 1123, 1181
HpyAV CCTTC 4 cut(s) 662, 814, 960, 1258
HpyCH4III ACNGT 4 cut(s) 331, 471, 1016, 1156
HpyCH4V TGCA 5 cut(s) 170, 174, 351, 1360, 1372
HpyF3I CTNAG 5 cut(s) 512, 683, 777, 827, 1292
Hsp92I GRCGYC 1 cut(s) 1118
Hsp92II CATG 4 cut(s) 174, 1192, 1252, 1376
HspAI GCGC 3 cut(s) 17, 831, 912
Kpn2I TCCGGA 1 cut(s) 475
KroI GCCGGC 3 cut(s) 377, 694, 833
KroNI GCCGGC 3 cut(s) 379, 696, 835
Ksp22I TGATCA 1 cut(s) 840
Kzo9I GATC 5 cut(s) 721, 840, 849, 855, 991
Lsp1109I GCAGC 6 cut(s) 6, 603, 746, 911, 990, 1098
LweI GCATC 4 cut(s) 157, 188, 864, 1282
MaeI CTAG 3 cut(s) 717, 933, 1328
MaeIII GTNAC 2 cut(s) 66, 706
MalI GATC 5 cut(s) 723, 842, 851, 857, 993
MbiI CCGCTC 3 cut(s) 147, 957, 972
MboI GATC 5 cut(s) 721, 840, 849, 855, 991
MboII GAAGA 9 cut(s) 16, 19, 32, 35, 40, 66, 646, 973, 1009
MfeI CAATTG 1 cut(s) 770
MhlI GDGCHC 6 cut(s) 103, 260, 585, 689, 791, 1187
MluCI AATT 5 cut(s) 110, 399, 586, 602, 770
MlyI GAGTC 1 cut(s) 201
Mph1103I ATGCAT 1 cut(s) 172
MreI CGCCGGCG 1 cut(s) 833
MroI TCCGGA 1 cut(s) 475
MroNI GCCGGC 3 cut(s) 377, 694, 833
MroXI GAANNNNTTC 1 cut(s) 638
MslI CAYNNNNRTG 3 cut(s) 1193, 1253, 1278
MspA1I CMGCKG 1 cut(s) 924
MspR9I CCNGG 6 cut(s) 97, 254, 464, 499, 661, 1148
MunI CAATTG 1 cut(s) 770
MvaI CCWGG 2 cut(s) 97, 1148
MvnI CGCG 5 cut(s) 219, 848, 914, 1049, 1214
NaeI GCCGGC 3 cut(s) 379, 696, 835
NciI CCSGG 4 cut(s) 254, 464, 499, 661
NcoI CCATGG 1 cut(s) 1188
NdeII GATC 5 cut(s) 721, 840, 849, 855, 991
NgoMIV GCCGGC 3 cut(s) 377, 694, 833
NlaIII CATG 4 cut(s) 174, 1192, 1252, 1376
NlaIV GGNNCC 9 cut(s) 101, 257, 258, 328, 468, 503, 518, 753, 988
NmeAIII GCCGAG 1 cut(s) 710
NmuCI GTSAC 2 cut(s) 66, 706
NruI TCGCGA 1 cut(s) 848
NsiI ATGCAT 1 cut(s) 172
NspI RCATGY 1 cut(s) 174
PaeI GCATGC 1 cut(s) 174
PcsI WCGNNNNNNNCGW 3 cut(s) 381, 447, 864
PdiI GCCGGC 3 cut(s) 379, 696, 835
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 1 cut(s) 266
PfoI TCCNGGA 1 cut(s) 659
PkrI GCNGC 9 cut(s) 21, 618, 736, 926, 929, 980, 1025, 1113, 1203
Ple19I CGATCG 1 cut(s) 852
PleI GAGTC 1 cut(s) 200
PpsI GAGTC 1 cut(s) 200
PshAI GACNNNNGTC 1 cut(s) 191
Psp124BI GAGCTC 1 cut(s) 585
Psp6I CCWGG 2 cut(s) 95, 1146
PspFI CCCAGC 1 cut(s) 764
PspGI CCWGG 2 cut(s) 95, 1146
PspN4I GGNNCC 9 cut(s) 101, 257, 258, 328, 468, 503, 518, 753, 988
PspOMI GGGCCC 2 cut(s) 99, 256
PspPI GGNCC 9 cut(s) 99, 100, 156, 186, 256, 257, 467, 901, 1040
PvuI CGATCG 1 cut(s) 852
RruI TCGCGA 1 cut(s) 848
RsaI GTAC 4 cut(s) 608, 1007, 1160, 1247
RsaNI GTAC 4 cut(s) 607, 1006, 1159, 1246
RseI CAYNNNNRTG 3 cut(s) 1193, 1253, 1278
SacI GAGCTC 1 cut(s) 585
SatI GCNGC 9 cut(s) 20, 617, 735, 925, 928, 979, 1024, 1112, 1202
Sau3AI GATC 5 cut(s) 721, 840, 849, 855, 991
Sau96I GGNCC 9 cut(s) 99, 100, 156, 186, 256, 257, 467, 901, 1040
SchI GAGTC 1 cut(s) 201
ScrFI CCNGG 6 cut(s) 97, 254, 464, 499, 661, 1148
SduI GDGCHC 6 cut(s) 103, 260, 585, 689, 791, 1187
SfaNI GCATC 4 cut(s) 157, 188, 864, 1282
SfcI CTRYAG 1 cut(s) 119
SgrAI CRCCGGYG 1 cut(s) 833
SinI GGWCC 3 cut(s) 156, 186, 467
SmiMI CAYNNNNRTG 3 cut(s) 1193, 1253, 1278
SmlI CTYRAG 3 cut(s) 359, 575, 1205
SmoI CTYRAG 3 cut(s) 359, 575, 1205
SphI GCATGC 1 cut(s) 174
Sse9I AATT 5 cut(s) 110, 399, 586, 602, 770
SspMI CTAG 3 cut(s) 717, 933, 1328
SstI GAGCTC 1 cut(s) 585
StyD4I CCNGG 6 cut(s) 95, 252, 462, 497, 659, 1146
StyI CCWWGG 2 cut(s) 1188, 1311
TaaI ACNGT 4 cut(s) 331, 471, 1016, 1156
TaqI TCGA 3 cut(s) 165, 300, 441
TaqII GACCGA 2 cut(s) 140, 1067
TasI AATT 5 cut(s) 110, 399, 586, 602, 770
TatI WGTACW 2 cut(s) 1158, 1245
TauI GCSGC 3 cut(s) 930, 1026, 1204
TfiI GAWTC 1 cut(s) 266
TscAI CASTG 1 cut(s) 1021
TseFI GTSAC 2 cut(s) 66, 706
TseI GCWGC 6 cut(s) 19, 616, 734, 924, 978, 1111
Tsp45I GTSAC 2 cut(s) 66, 706
TspDTI ATGAA 2 cut(s) 17, 729
TspGWI ACGGA 3 cut(s) 425, 624, 999
TspRI CASTG 1 cut(s) 1021
VpaK11BI GGWCC 3 cut(s) 156, 186, 467
XapI RAATTY 1 cut(s) 399
XceI RCATGY 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 3 cut(s) 717, 933, 1328
Zsp2I ATGCAT 1 cut(s) 172
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.