Rroxscaffold_6G00409590

Leucine-rich repeat receptor-like protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0012382
Physical Location & Seq
Forward (+)
32108046 .. 32110604
2559 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00409590.1

Sequence Viewer

Length: 2034 bp
ATGAAGAAGGAGGCTTATCCTATAGCCTTAGCCTTCCTACTCCTACTCACCTCGGCTAAACTCTCACTCTCCTCTTCGCCGCCGAACGACACGTCAGCTCTCACCGACTTCCGCCTCAAAACCGACACCCACGGCTACCTCCGATCCAACTGGACCGGACCCGACCCTTGCGCCGCTGCATGGGTCGGAGTCCGATGCTCCAATAACAACCTCCGAGTCGTCGGTCTCTCCCTTCCTTCCCTCAACCTGCGCGGCCCGCTCGACTCTCTGGCTCAGCTGGACCAACTCCGCCTTCTTGACCTCCACAACAACCGCCTCAACGGCACCGTTTTGCCCTTAGCCAACTGCACCAACCTCAAGCTGCTCTACCTCGCCGGGAACGACCTCTCCGGCGAGATCCCGCCGGAATTTGCCTCTCTCAGGCGGCTTCTCCGTCTAGACCTCTCCGACAACAACATCCGCGGCGCGGTCCCCGACGGTATCTCCCGCCTGAGCCGGCTCCTCACGCTCCGGCTCCAGAACAACCTCCTCACCGGCAAGATCCCCGACCTCTCCGGCTCCCTCTCCGACCTGAAGGAGCTTAACCTGACCAACAACGAGTTCTATGGAAAATTGCCGGAGGGTTTGCTCAGAAAATTCGGTCAGGAAAGCTTTTCCGGCAACGAAGGGCTCTGCGGCTCTTCTCCGTTACCGGCTTGCTCCTTCACCGGAGCTAACCCACCCTCGGCGGCTTCGACCGAAACGGTGCCGTCTAACCCCAGCTCGTTGCCGCAAACAAGCATCATAAACGAGCCGAAGGTCAAAAACTCGAGAAAGGGATTGAGTCCTGGAGCAATAGTAGCAATTGTGATAGCGAATTGCGTAGCCATTCTCGTTATCATTTCGTTCATTGTAGCTCACTACTGCGCCAGAGACCGAAGCTCGAACTCGATCGGGGGAAGCGAAGCCGGGAAGAGGAAGAGTGGGAGTAGCTATGGAGGTGAAAAGAGGGTGTATGCTAACAATGGGGGTGGTGACAGTGATGGGACTAATGCTACTGACCGGAGTAAGCTGGTGTTTTTCGACCGGAGGAAGCAGTTTGAGCTCGAGGACTTGCTCCGGGCGTCGGCGGAGATGTTGGGGAAAGGGAGCTTGGGGACGGTGTACAAGGCGGTGCTTGATGATGGTTGTACAATGGCGGTGAAGCGGCTCAAGGATGCGAACCCTTGTGCGAGGAAAGAGTTTGAGCAGTATATGGATTTGATTGGGAAGATTAAGCACCCTAATGTGGTGAGACTTAGTGCTTATTACTATGCTAAGGAGGAGAAGCTTCTTGTCTATGACTATCTGCCTAATGGGAGCCTGCACTCACTTCTTCATGAGAATCGCGGTCCGGGCAGAATTCCTTTGGATTGGACTACAAGGATCAGCTTGGTGTTGGGAGCTGCCCGCGGACTAGCCCGGATTCATGAAGAGTTTAGCGCTGCAAAAGTACCACATGGGAATGTGAAGTCTTCCAATGTGCTCCTTGACAAGAACGGTGTGGCTTGCATTTCTGACTTCGGACTGTCCCTGCTTCTGAACCCAGTTCACGCCATTGCGAGGTTGGGAGGATACAGAGCTCCTGAGCAGGCAGAGACCAAGAGGCTATCGCAGACAGCAGACGTGTACAGCTTCGGGGTCTTGTTGTTGGAGGTTCTCACAGGAAGAGCTCCTTCCCTGTACCCTTCTCCTACTCGCCCACGCATGGATGAGGAGGAGCAGGCGGTGGACCTGCCGAAATGGGTGAGGTCTGTGGTGAAGGAAGAGTGGACTGGAGAGGTGTTTGATCAGGAACTTCTGAGGTACAAGAACATCGAGGAGGAGCTTGTGTCAATGCTCCATGTGGGATTGGCATGCGTGGTGCCTCAGCCAGAAAAGAGGCCTACTATGGTTGAAGTGGCTAAGATGGTTGAGGACATAAGAGTGGAGAGATCTCCTCTTGGGGAAGACTATGATGATTCCCGCAATTCGCTTTCTCCTTCCATTCCCACCACCGAAGATGGGCTAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0008150 GO:0009832 GO:0009834 GO:0009987 GO:0016020 GO:0042546 GO:0044085 GO:0044464 GO:0071554 GO:0071669 GO:0071840 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_4 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

677

Amino Acids

73.82

Weight (kDa)

7.9

Isoelectric Point (pI)

43.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 89 - 198 5.6e-08 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 371 - 642 8.7e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 371 - 642 3.1e-32 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012382)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G36570
fragaria_vesca FvH4_6g17500
malus_domestica MD12G1097300.v1.1 MD14G1094000.v1.1
prunus_persica Prupe.7G120500_v2.0.a1
pyrus_communis pycom12g09760 pycom14g09180
rosa_chinensis RchiOBHm_Chr3g0471741
rosa_laevigata RLG00000024137
rosa_multiflora Rmu_co8460341.1_g000001 Rmu_sc0013806.1_g000002
rosa_roxburghii Rroxscaffold_6G00409590
rosa_rugosa Rorug03G0119500
rosa_samantha Rh3AG170800 Rh3BG196500 Rh3CG187200 Rh3DG178600
rosa_wichuraiana Rw0G009060 Rw3G016950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 255, 1761
AccB1I GGYRCC 3 cut(s) 323, 745, 1882
AccBSI CCGCTC 1 cut(s) 259
AccII CGCG 5 cut(s) 252, 462, 467, 1368, 1431
AclWI GGATC 4 cut(s) 138, 391, 535, 1412
AcsI RAATTY 3 cut(s) 407, 635, 1380
AcuI CTGAAG 1 cut(s) 593
AcyI GRCGYC 1 cut(s) 1103
AfaI GTAC 6 cut(s) 1145, 1171, 1473, 1649, 1703, 1826
AfeI AGCGCT 1 cut(s) 1462
AflIII ACRYGT 2 cut(s) 90, 1644
AgsI TTSAA 1 cut(s) 1916
AjiI CACGTC 2 cut(s) 93, 1645
AjnI CCWGG 1 cut(s) 826
AjuI GAANNNNNNNTTGG 6 cut(s) 276, 308, 584, 616, 1115, 1147
AloI GAACNNNNNNTCC 2 cut(s) 371, 403
Alw21I GWGCWC 4 cut(s) 1086, 1506, 1603, 1693
Alw26I GTCTC 4 cut(s) 230, 906, 1267, 1610
AlwI GGATC 4 cut(s) 138, 391, 535, 1412
Ama87I CYCGRG 2 cut(s) 808, 1085
Aor51HI AGCGCT 1 cut(s) 1462
AoxI GGCC 2 cut(s) 253, 1901
ApeKI GCWGC 4 cut(s) 176, 361, 1424, 1463
ApoI RAATTY 3 cut(s) 407, 635, 1380
ArsI GACNNNNNNTTYG 2 cut(s) 77, 109
AspLEI GCGC 5 cut(s) 173, 252, 467, 908, 1463
AspS9I GGNCC 7 cut(s) 153, 158, 254, 280, 469, 1370, 1750
AsuC2I CCSGG 5 cut(s) 376, 949, 1100, 1374, 1441
AsuNHI GCTAGC 1 cut(s) 2026
AvaI CYCGRG 2 cut(s) 808, 1085
AvaII GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
BanI GGYRCC 3 cut(s) 323, 745, 1882
BanII GRGCYC 4 cut(s) 672, 1086, 1603, 1693
BbsI GAAGAC 2 cut(s) 1485, 1974
Bbv12I GWGCWC 4 cut(s) 1086, 1506, 1603, 1693
BbvCI CCTCAGC 1 cut(s) 1887
BbvI GCAGC 4 cut(s) 163, 348, 1411, 1450
BccI CCATC 4 cut(s) 1016, 1157, 1921, 2015
BceAI ACGGC 3 cut(s) 148, 337, 733
BciT130I CCWGG 1 cut(s) 828
BciVI GTATCC 1 cut(s) 1586
BclI TGATCA 1 cut(s) 1807
BcnI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1441
BcoDI GTCTC 4 cut(s) 230, 906, 1267, 1610
BfaI CTAG 3 cut(s) 437, 1436, 2027
BfmI CTRYAG 1 cut(s) 21
BfoI RGCGCY 1 cut(s) 1464
BfuAI ACCTGC 2 cut(s) 255, 1761
BfuI GTATCC 1 cut(s) 1586
BglI GCCNNNNNGGC 1 cut(s) 321
BglII AGATCT 1 cut(s) 1952
BlpI GCTNAGC 1 cut(s) 273
Bme1390I CCNGG 6 cut(s) 376, 828, 949, 1100, 1374, 1441
Bme18I GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
BmeT110I CYCGRG 2 cut(s) 808, 1085
BmgBI CACGTC 2 cut(s) 93, 1645
BmgT120I GGNCC 7 cut(s) 153, 158, 254, 280, 469, 1370, 1750
BmiI GGNNCC 9 cut(s) 160, 325, 471, 500, 515, 559, 747, 1340, 1884
BmrFI CCNGG 6 cut(s) 376, 828, 949, 1100, 1374, 1441
BmrI ACTGGG 1 cut(s) 1559
BmsI GCATC 3 cut(s) 185, 789, 1186
BmtI GCTAGC 1 cut(s) 2030
BmuI ACTGGG 1 cut(s) 1559
BpiI GAAGAC 2 cut(s) 1485, 1974
BplI GAGNNNNNCTC 2 cut(s) 1942, 1974
BpmI CTGGAG 3 cut(s) 500, 849, 1815
Bpu10I CCTNAGC 6 cut(s) 28, 337, 491, 1296, 1605, 1887
Bpu1102I GCTNAGC 1 cut(s) 273
BpuEI CTTGAG 2 cut(s) 341, 1175
BpuMI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1441
BsaHI GRCGYC 1 cut(s) 1103
BsaI GGTCTC 3 cut(s) 230, 906, 1610
BsaJI CCNNGG 5 cut(s) 51, 130, 460, 723, 1429
BsaWI WCCGGW 4 cut(s) 155, 707, 1041, 1065
BsaXI ACNNNNNCTCC 8 cut(s) 371, 401, 467, 497, 822, 852, 1835, 1865
Bse118I RCCGGY 3 cut(s) 495, 533, 691
Bse1I ACTGG 3 cut(s) 155, 1565, 1798
Bse3DI GCAATG 1 cut(s) 1575
BseBI CCWGG 1 cut(s) 828
BseDI CCNNGG 5 cut(s) 51, 130, 460, 723, 1429
BseGI GGATG 3 cut(s) 456, 1201, 1735
BseMI GCAATG 1 cut(s) 1575
BseMII CTCAG 7 cut(s) 287, 433, 482, 643, 1596, 1811, 1901
BseNI ACTGG 3 cut(s) 155, 1565, 1798
BseRI GAGGAG 9 cut(s) 61, 491, 518, 1316, 1748, 1751, 1853, 1856, 1947
BseXI GCAGC 4 cut(s) 163, 348, 1411, 1450
BseYI CCCAGC 1 cut(s) 758
BsgI GTGCAG 2 cut(s) 331, 1328
Bsh1236I CGCG 5 cut(s) 252, 462, 467, 1368, 1431
Bsh1285I CGRYCG 3 cut(s) 738, 933, 1066
BshFI GGCC 2 cut(s) 255, 1903
BshNI GGYRCC 3 cut(s) 323, 745, 1882
BsiEI CGRYCG 3 cut(s) 738, 933, 1066
BsiHKAI GWGCWC 4 cut(s) 1086, 1506, 1603, 1693
BsiHKCI CYCGRG 2 cut(s) 808, 1085
BslFI GGGAC 4 cut(s) 455, 1039, 1150, 1534
BsmAI GTCTC 4 cut(s) 230, 906, 1267, 1610
BsmFI GGGAC 4 cut(s) 455, 1039, 1150, 1534
BsnI GGCC 2 cut(s) 255, 1903
Bso31I GGTCTC 3 cut(s) 230, 906, 1610
BsoBI CYCGRG 2 cut(s) 808, 1085
Bsp1286I GDGCHC 5 cut(s) 672, 1086, 1506, 1603, 1693
Bsp1407I TGTACA 3 cut(s) 1143, 1169, 1647
Bsp143I GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
Bsp1720I GCTNAGC 1 cut(s) 273
BspANI GGCC 2 cut(s) 255, 1903
BspCNI CTCAG 7 cut(s) 286, 432, 483, 642, 1597, 1812, 1900
BspFNI CGCG 5 cut(s) 252, 462, 467, 1368, 1431
BspHI TCATGA 2 cut(s) 1357, 1447
BspLI GGNNCC 9 cut(s) 160, 325, 471, 500, 515, 559, 747, 1340, 1884
BspMI ACCTGC 2 cut(s) 255, 1761
BspOI GCTAGC 1 cut(s) 2030
BspPI GGATC 4 cut(s) 138, 391, 535, 1412
BspQI GCTCTTC 2 cut(s) 685, 1681
BspT107I GGYRCC 3 cut(s) 323, 745, 1882
BspTNI GGTCTC 3 cut(s) 230, 906, 1610
BsrBI CCGCTC 1 cut(s) 259
BsrDI GCAATG 1 cut(s) 1575
BsrFI RCCGGY 3 cut(s) 495, 533, 691
BsrGI TGTACA 3 cut(s) 1143, 1169, 1647
BsrI ACTGG 3 cut(s) 155, 1565, 1798
BssAI RCCGGY 3 cut(s) 495, 533, 691
BssECI CCNNGG 5 cut(s) 51, 130, 460, 723, 1429
BssMI GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
BssNI GRCGYC 1 cut(s) 1103
Bst2UI CCWGG 1 cut(s) 828
Bst4CI ACNGT 7 cut(s) 328, 479, 745, 1019, 1141, 1520, 1548
Bst6I CTCTTC 7 cut(s) 79, 685, 947, 953, 1446, 1681, 1779
BstACI GRCGYC 1 cut(s) 1103
BstAUI TGTACA 3 cut(s) 1143, 1169, 1647
BstDSI CCRYGG 3 cut(s) 130, 460, 1429
BstENI CCTNNNNNAGG 1 cut(s) 418
BstF5I GGATG 3 cut(s) 456, 1201, 1735
BstFNI CGCG 5 cut(s) 252, 462, 467, 1368, 1431
BstH2I RGCGCY 1 cut(s) 1464
BstHHI GCGC 5 cut(s) 173, 252, 467, 908, 1463
BstKTI GATC 7 cut(s) 146, 399, 543, 933, 1407, 1810, 1955
BstMAI GTCTC 4 cut(s) 230, 906, 1267, 1610
BstMBI GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
BstMCI CGRYCG 3 cut(s) 738, 933, 1066
BstMWI GCNNNNNNNGC 7 cut(s) 256, 321, 505, 657, 839, 1081, 1374
BstNI CCWGG 1 cut(s) 828
BstNSI RCATGY 1 cut(s) 1878
BstSCI CCNGG 6 cut(s) 374, 826, 947, 1098, 1372, 1439
BstSFI CTRYAG 1 cut(s) 21
BstUI CGCG 5 cut(s) 252, 462, 467, 1368, 1431
BstV1I GCAGC 4 cut(s) 163, 348, 1411, 1450
BstV2I GAAGAC 2 cut(s) 1485, 1974
BstX2I RGATCY 3 cut(s) 396, 540, 1952
BstYI RGATCY 3 cut(s) 396, 540, 1952
BsuI GTATCC 1 cut(s) 1586
BsuRI GGCC 2 cut(s) 255, 1903
BtgI CCRYGG 3 cut(s) 130, 460, 1429
BtrI CACGTC 2 cut(s) 93, 1645
BtsCI GGATG 3 cut(s) 456, 1201, 1735
BtsIMutI CAGTG 1 cut(s) 1024
BveI ACCTGC 2 cut(s) 255, 1761
CciI TCATGA 2 cut(s) 1357, 1447
CfoI GCGC 5 cut(s) 173, 252, 467, 908, 1463
Cfr10I RCCGGY 3 cut(s) 495, 533, 691
Cfr13I GGNCC 7 cut(s) 153, 158, 254, 280, 469, 1370, 1750
Cfr42I CCGCGG 2 cut(s) 463, 1432
CpoI CGGWCCG 1 cut(s) 1370
CseI GACGC 1 cut(s) 1092
Csp6I GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
CspCI CAANNNNNGTGG 2 cut(s) 991, 1026
CspI CGGWCCG 1 cut(s) 1370
CviAII CATG 7 cut(s) 180, 1358, 1448, 1478, 1726, 1862, 1875
CviQI GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
DpnI GATC 7 cut(s) 145, 398, 542, 932, 1406, 1809, 1954
DpnII GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
Eam1104I CTCTTC 7 cut(s) 79, 685, 947, 953, 1446, 1681, 1779
EarI CTCTTC 7 cut(s) 79, 685, 947, 953, 1446, 1681, 1779
EciI GGCGGA 3 cut(s) 101, 278, 1124
Ecl136II GAGCTC 3 cut(s) 1084, 1601, 1691
Eco147I AGGCCT 1 cut(s) 1903
Eco24I GRGCYC 4 cut(s) 672, 1086, 1603, 1693
Eco31I GGTCTC 3 cut(s) 230, 906, 1610
Eco47I GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
Eco47III AGCGCT 1 cut(s) 1462
Eco53kI GAGCTC 3 cut(s) 1084, 1601, 1691
Eco57I CTGAAG 1 cut(s) 593
Eco88I CYCGRG 2 cut(s) 808, 1085
EcoICRI GAGCTC 3 cut(s) 1084, 1601, 1691
EcoNI CCTNNNNNAGG 1 cut(s) 418
EcoRI GAATTC 1 cut(s) 1380
EcoRII CCWGG 1 cut(s) 826
EcoT38I GRGCYC 4 cut(s) 672, 1086, 1603, 1693
FaeI CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
FalI AAGNNNNNCTT 2 cut(s) 1678, 1710
FaqI GGGAC 4 cut(s) 455, 1039, 1150, 1534
FatI CATG 7 cut(s) 179, 1357, 1447, 1477, 1725, 1861, 1874
FauI CCCGC 5 cut(s) 264, 408, 494, 1436, 1991
FbaI TGATCA 1 cut(s) 1807
FokI GGATG 3 cut(s) 443, 1208, 1742
FriOI GRGCYC 4 cut(s) 672, 1086, 1603, 1693
FspBI CTAG 3 cut(s) 437, 1436, 2027
GlaI GCGC 5 cut(s) 172, 251, 466, 907, 1462
GsaI CCCAGC 1 cut(s) 762
GsuI CTGGAG 3 cut(s) 500, 849, 1815
HaeII RGCGCY 1 cut(s) 1464
HaeIII GGCC 2 cut(s) 255, 1903
HgaI GACGC 1 cut(s) 1092
HhaI GCGC 5 cut(s) 173, 252, 467, 908, 1463
Hin1I GRCGYC 1 cut(s) 1103
Hin1II CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
Hin6I GCGC 5 cut(s) 171, 250, 465, 906, 1461
HinP1I GCGC 5 cut(s) 171, 250, 465, 906, 1461
HindIII AAGCTT 2 cut(s) 649, 1307
HinfI GANTC 7 cut(s) 189, 216, 263, 823, 1363, 1444, 1979
Hpy166II GTNNAC 5 cut(s) 1144, 1570, 1648, 1750, 1791
Hpy188III TCNNGA 9 cut(s) 296, 437, 517, 644, 810, 1358, 1448, 1604, 1811
Hpy8I GTNNAC 5 cut(s) 1144, 1570, 1648, 1750, 1791
Hpy99I CGWCG 3 cut(s) 224, 479, 1108
HpyCH4III ACNGT 7 cut(s) 328, 479, 745, 1019, 1141, 1520, 1548
HpyCH4IV ACGT 2 cut(s) 92, 1644
HpyCH4V TGCA 5 cut(s) 179, 348, 1345, 1466, 1530
HpyF10VI GCNNNNNNNGC 7 cut(s) 256, 321, 505, 657, 839, 1081, 1374
HpySE526I ACGT 2 cut(s) 92, 1644
Hsp92I GRCGYC 1 cut(s) 1103
Hsp92II CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
HspAI GCGC 5 cut(s) 171, 250, 465, 906, 1461
KroI GCCGGC 1 cut(s) 495
KroNI GCCGGC 1 cut(s) 497
Ksp22I TGATCA 1 cut(s) 1807
KspI CCGCGG 2 cut(s) 463, 1432
Kzo9I GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
LguI GCTCTTC 2 cut(s) 685, 1681
Lsp1109I GCAGC 4 cut(s) 163, 348, 1411, 1450
LweI GCATC 3 cut(s) 185, 789, 1186
MaeI CTAG 3 cut(s) 437, 1436, 2027
MaeII ACGT 2 cut(s) 92, 1644
MaeIII GTNAC 2 cut(s) 687, 1013
MalI GATC 7 cut(s) 145, 398, 542, 932, 1406, 1809, 1954
MbiI CCGCTC 1 cut(s) 259
MboI GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
MfeI CAATTG 1 cut(s) 843
MflI RGATCY 3 cut(s) 396, 540, 1952
MhlI GDGCHC 5 cut(s) 672, 1086, 1506, 1603, 1693
MluCI AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
MlyI GAGTC 4 cut(s) 198, 225, 257, 832
MmeI TCCRAC 5 cut(s) 166, 171, 471, 591, 1650
MroNI GCCGGC 1 cut(s) 495
MseI TTAA 2 cut(s) 582, 1254
MslI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
MspA1I CMGCKG 4 cut(s) 176, 277, 462, 1431
MspR9I CCNGG 6 cut(s) 376, 828, 949, 1100, 1374, 1441
MunI CAATTG 1 cut(s) 843
MvaI CCWGG 1 cut(s) 828
MvnI CGCG 5 cut(s) 252, 462, 467, 1368, 1431
MwoI GCNNNNNNNGC 7 cut(s) 256, 321, 505, 657, 839, 1081, 1374
NaeI GCCGGC 1 cut(s) 497
NciI CCSGG 5 cut(s) 376, 949, 1100, 1374, 1441
NdeII GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
NgoMIV GCCGGC 1 cut(s) 495
NheI GCTAGC 1 cut(s) 2026
NlaIII CATG 7 cut(s) 183, 1361, 1451, 1481, 1729, 1865, 1878
NlaIV GGNNCC 9 cut(s) 160, 325, 471, 500, 515, 559, 747, 1340, 1884
NmeAIII GCCGAG 2 cut(s) 32, 704
NmuCI GTSAC 1 cut(s) 1013
NspI RCATGY 1 cut(s) 1878
PaeI GCATGC 1 cut(s) 1878
PaeR7I CTCGAG 2 cut(s) 808, 1085
PagI TCATGA 2 cut(s) 1357, 1447
PceI AGGCCT 1 cut(s) 1903
PciSI GCTCTTC 2 cut(s) 685, 1681
PcsI WCGNNNNNNNCGW 3 cut(s) 378, 731, 939
PdiI GCCGGC 1 cut(s) 497
PfeI GAWTC 3 cut(s) 1363, 1444, 1979
PfoI TCCNGGA 1 cut(s) 826
Ple19I CGATCG 1 cut(s) 933
PleI GAGTC 4 cut(s) 197, 224, 257, 831
PpsI GAGTC 4 cut(s) 197, 224, 257, 831
Psp124BI GAGCTC 3 cut(s) 1086, 1603, 1693
Psp6I CCWGG 1 cut(s) 826
PspFI CCCAGC 1 cut(s) 758
PspGI CCWGG 1 cut(s) 826
PspN4I GGNNCC 9 cut(s) 160, 325, 471, 500, 515, 559, 747, 1340, 1884
PspPI GGNCC 7 cut(s) 153, 158, 254, 280, 469, 1370, 1750
PspXI VCTCGAGB 1 cut(s) 1085
PsuI RGATCY 3 cut(s) 396, 540, 1952
PvuI CGATCG 1 cut(s) 933
PvuII CAGCTG 1 cut(s) 277
RsaI GTAC 6 cut(s) 1145, 1171, 1473, 1649, 1703, 1826
RsaNI GTAC 6 cut(s) 1144, 1170, 1472, 1648, 1702, 1825
RseI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
Rsr2I CGGWCCG 1 cut(s) 1370
RsrII CGGWCCG 1 cut(s) 1370
SacI GAGCTC 3 cut(s) 1086, 1603, 1693
SacII CCGCGG 2 cut(s) 463, 1432
SapI GCTCTTC 2 cut(s) 685, 1681
SaqAI TTAA 2 cut(s) 582, 1254
Sau3AI GATC 7 cut(s) 143, 396, 540, 930, 1404, 1807, 1952
Sau96I GGNCC 7 cut(s) 153, 158, 254, 280, 469, 1370, 1750
SchI GAGTC 4 cut(s) 198, 225, 257, 832
ScrFI CCNGG 6 cut(s) 376, 828, 949, 1100, 1374, 1441
SduI GDGCHC 5 cut(s) 672, 1086, 1506, 1603, 1693
SfaNI GCATC 3 cut(s) 185, 789, 1186
SfcI CTRYAG 1 cut(s) 21
Sfr274I CTCGAG 2 cut(s) 808, 1085
Sfr303I CCGCGG 2 cut(s) 463, 1432
SgrBI CCGCGG 2 cut(s) 463, 1432
SinI GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
SlaI CTCGAG 2 cut(s) 808, 1085
SmiMI CAYNNNNRTG 3 cut(s) 1263, 1482, 1943
SmlI CTYRAG 4 cut(s) 356, 808, 1085, 1190
SmoI CTYRAG 4 cut(s) 356, 808, 1085, 1190
SphI GCATGC 1 cut(s) 1878
Sse9I AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
SseBI AGGCCT 1 cut(s) 1903
SspMI CTAG 3 cut(s) 437, 1436, 2027
SstI GAGCTC 3 cut(s) 1086, 1603, 1693
StuI AGGCCT 1 cut(s) 1903
StyD4I CCNGG 6 cut(s) 374, 826, 947, 1098, 1372, 1439
TaaI ACNGT 7 cut(s) 328, 479, 745, 1019, 1141, 1520, 1548
TaiI ACGT 2 cut(s) 95, 1647
TaqI TCGA 8 cut(s) 261, 734, 809, 923, 929, 1062, 1086, 1836
TaqII GACCGA 4 cut(s) 212, 629, 752, 930
TasI AATT 7 cut(s) 407, 611, 635, 843, 856, 1380, 1987
TatI WGTACW 3 cut(s) 1143, 1169, 1647
TauI GCSGC 9 cut(s) 82, 176, 255, 427, 465, 678, 731, 772, 1189
TfiI GAWTC 3 cut(s) 1363, 1444, 1979
Tru1I TTAA 2 cut(s) 582, 1254
Tru9I TTAA 2 cut(s) 582, 1254
TscAI CASTG 1 cut(s) 1024
TseFI GTSAC 1 cut(s) 1013
TseI GCWGC 4 cut(s) 176, 361, 1424, 1463
Tsp45I GTSAC 1 cut(s) 1013
TspDTI ATGAA 5 cut(s) 17, 877, 1346, 1436, 1464
TspGWI ACGGA 2 cut(s) 422, 675
TspRI CASTG 1 cut(s) 1024
VpaK11BI GGWCC 6 cut(s) 153, 158, 280, 469, 1370, 1750
XagI CCTNNNNNAGG 1 cut(s) 418
XapI RAATTY 3 cut(s) 407, 635, 1380
XbaI TCTAGA 1 cut(s) 436
XceI RCATGY 1 cut(s) 1878
XcmI CCANNNNNNNNNTGG 1 cut(s) 1582
XhoI CTCGAG 2 cut(s) 808, 1085
XspI CTAG 3 cut(s) 437, 1436, 2027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.