pycom12g19260

NHL repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0008360
Physical Location & Seq
Reverse (-)
21160289 .. 21163489
3201 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1542 bp
ATGTTGGACAATGCACCGATTCCTTTAAATTTGGAGAAAGATCGTGATCCTCGTTTAGTTACATCCCCGCTGAAGTTTCCAGGAAAGCTTGCAATTGATGTTCTAAATGATAGGCTCTTCATTTCAGACAGTAATCATAACCGCATAGTGGTAACCGACCTAGATGGAAATTTTATTGTCCAAATAGGAAGTAGTGGAGAGGAAGGTTTTCGTGATGGTTCATTTGATGATGCCACCTTCAATCGGCCTCAGGGCCTAGCTTACAACCCAAGGAAAAACCTCCTCTATGTTGCAGATACTGAAAACCATGCTTTGAGGGAGATTGATTTTGTTAATGAGACAGTCCATACTCTTGCTGGAAATGGAACCAAAGGCTCTGACTACCGAGGGGGAGGGAAAGGAAGTACTCAGCTCCTCAACTCACCATGGGACATGTGCTTTCATCCAGTCAATGAGAAAGTTTATATTGCCATGGCTGGCTCGCATCAAATCTGGGAGCACAATACAGATGATGGAGCTACTAGATCATTTAGTGGTGATGGTTACGAAAGAAACTTGAATGGATCAAGCTCTTCAAGCACATCGTTCGCACAGCCTTCTGGAATTTCATTATCTCTTGATCTCAGGGAGCTATATATTGCTGATAGTGAGAGTAGCTCCATTAGGGCACTTGATTTAAAAACAGGAGGGTCGAAATTGCTAGCTGGTGGTGATCCATATTTTGCGGAAAATTTGTTTAAGTTTGGTGACCACGATGGAATAGGGTCTGAAGTACTTCTTCAACACCCCCTAGGAGTCTTATGTGCAAAGAATGGTGAAGTTTATATAGCAGACAGTTACAATCACAAGATAAAGAAACTAGATCCAGCTGCTAAAAGAGTGAGTACTGTAGCAGGGACAGGAAAAGCTGGCTTCAAGGACGGAACATCTCTAGAAGCTCAGCTTTCAGAGCCATCAGGAATTGTTGAATCAAAAAACGGAAGAATTTTTGTAGCTGATACAAACAACAGTCTAATCAGATATCTAGACTTAAACAGGGAAGAAGCTGAACTTCTTACTTTGGAGCTGAAAGGGGTTCAGCCTCCCGTAGCAAAATCCAAGTCTTTGAAACGCCTCAGAAGGCGGTCATCAGCTGACACGCAGACCATTACAGTTGATGGCGGCCCATCCAGCGAGGGCAACCTGTCTATTAAAATATCAGTACCTGAAGGGTATCATTTTTCAAAGGAAGCTCGCAGTAAGTTTAGCGTTGAAACTGAGCCTGAAACTGCAGTTTCCATTGAGCCCGTTGATGGATATCTTAGTCCAGAAGGATCAGCAACTCTTCATTTCAAGAGACCATCTCCCTCAACTTCAATGGGGAGAATAAATTGCAAGGTTTACTATTGCAAGGAAGATGAGGTATGCTTATACCAATCGTTATTATTCGAAGTACCATTTCTCGAGGAAATTCCAGGATCCAACCCAGAAGAAATCACACTGGCACACGTTGTAAAGCCGAAAACTTCAACAAGCAGCTTACAACTACCGGATGCGCGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
HAD_2 NHL Thioredoxin_8

Protein Analysis

514

Amino Acids

55.9

Weight (kDa)

5.37

Isoelectric Point (pI)

35.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NHL PF01436 80 - 106 2.2e-06 NHL repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 1537
AciI CCGC 5 cut(s) 68, 142, 725, 1123, 1161
AclWI GGATC 7 cut(s) 41, 571, 707, 857, 1321, 1452, 1465
AcsI RAATTY 6 cut(s) 28, 169, 603, 730, 984, 1449
AcuI CTGAAG 3 cut(s) 92, 789, 1227
AfaI GTAC 5 cut(s) 406, 774, 886, 1203, 1434
AfiI CCNNNNNNNGG 3 cut(s) 148, 243, 1292
AflIII ACRYGT 2 cut(s) 432, 1486
AjnI CCWGG 2 cut(s) 79, 1453
Alw21I GWGCWC 1 cut(s) 501
Alw26I GTCTC 2 cut(s) 332, 1330
AlwI GGATC 7 cut(s) 41, 571, 707, 857, 1321, 1452, 1465
AlwNI CAGNNNCTG 2 cut(s) 299, 1205
Ama87I CYCGRG 1 cut(s) 1442
AoxI GGCC 3 cut(s) 245, 253, 1162
ApeKI GCWGC 2 cut(s) 869, 1515
ApoI RAATTY 6 cut(s) 28, 169, 603, 730, 984, 1449
ArsI GACNNNNNNTTYG 2 cut(s) 1085, 1117
Asp700I GAANNNNTTC 2 cut(s) 207, 774
AspA2I CCTAGG 1 cut(s) 790
AspLEI GCGC 2 cut(s) 1537, 1539
AspS9I GGNCC 2 cut(s) 253, 1163
AsuHPI GGTGA 5 cut(s) 414, 548, 722, 758, 827
AsuII TTCGAA 1 cut(s) 1428
AsuNHI GCTAGC 1 cut(s) 700
AvaI CYCGRG 1 cut(s) 1442
AvrII CCTAGG 1 cut(s) 790
AxyI CCTNAGG 1 cut(s) 249
BaeGI GKGCMC 1 cut(s) 670
BamHI GGATCC 1 cut(s) 1457
BanII GRGCYC 1 cut(s) 1287
Bbv12I GWGCWC 1 cut(s) 501
BbvI GCAGC 2 cut(s) 856, 1527
BcgI CGANNNNNNTGC 2 cut(s) 568, 602
BciT130I CCWGG 2 cut(s) 81, 1455
BcoDI GTCTC 2 cut(s) 332, 1330
BfaI CTAG 8 cut(s) 161, 257, 522, 701, 791, 860, 932, 1025
BfmI CTRYAG 2 cut(s) 888, 1269
BisI GCNGC 3 cut(s) 870, 1162, 1516
BlnI CCTAGG 1 cut(s) 790
BlpI GCTNAGC 1 cut(s) 939
BlsI GCNGC 3 cut(s) 871, 1163, 1517
BmcAI AGTACT 3 cut(s) 406, 774, 886
Bme1390I CCNGG 2 cut(s) 81, 1455
BmeT110I CYCGRG 1 cut(s) 1442
BmgT120I GGNCC 2 cut(s) 253, 1163
BmiI GGNNCC 2 cut(s) 367, 1459
BmrFI CCNGG 2 cut(s) 81, 1455
BmsI GCATC 3 cut(s) 220, 493, 1522
BmtI GCTAGC 1 cut(s) 704
BplI GAGNNNNNCTC 4 cut(s) 641, 673, 1327, 1359
Bpu1102I GCTNAGC 1 cut(s) 939
Bpu14I TTCGAA 1 cut(s) 1428
BsaBI GATNNNNATC 2 cut(s) 45, 1296
BsaI GGTCTC 1 cut(s) 1330
BsaJI CCNNGG 5 cut(s) 269, 385, 425, 471, 790
BsaWI WCCGGW 1 cut(s) 1528
Bsc4I CCNNNNNNNGG 3 cut(s) 148, 243, 1292
Bse1I ACTGG 2 cut(s) 446, 1485
Bse21I CCTNAGG 1 cut(s) 249
Bse8I GATNNNNATC 2 cut(s) 45, 1296
BseBI CCWGG 2 cut(s) 81, 1455
BseDI CCNNGG 5 cut(s) 269, 385, 425, 471, 790
BseGI GGATG 4 cut(s) 62, 442, 1166, 1537
BseJI GATNNNNATC 2 cut(s) 45, 1296
BseLI CCNNNNNNNGG 3 cut(s) 148, 243, 1292
BseMII CTCAG 6 cut(s) 263, 422, 637, 953, 1129, 1248
BseNI ACTGG 2 cut(s) 446, 1485
BsePI GCGCGC 1 cut(s) 1535
BseRI GAGGAG 2 cut(s) 272, 404
BseSI GKGCMC 1 cut(s) 670
BseXI GCAGC 2 cut(s) 856, 1527
Bsh1236I CGCG 1 cut(s) 1537
BshFI GGCC 3 cut(s) 247, 255, 1164
BsiHKAI GWGCWC 1 cut(s) 501
BsiHKCI CYCGRG 1 cut(s) 1442
BsiSI CCGG 1 cut(s) 1529
BslFI GGGAC 2 cut(s) 443, 910
BslI CCNNNNNNNGG 3 cut(s) 148, 243, 1292
BsmAI GTCTC 2 cut(s) 332, 1330
BsmFI GGGAC 2 cut(s) 443, 910
BsnI GGCC 3 cut(s) 247, 255, 1164
Bso31I GGTCTC 1 cut(s) 1330
BsoBI CYCGRG 1 cut(s) 1442
Bsp119I TTCGAA 1 cut(s) 1428
Bsp1286I GDGCHC 3 cut(s) 501, 670, 1287
Bsp143I GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
Bsp1720I GCTNAGC 1 cut(s) 939
Bsp19I CCATGG 2 cut(s) 425, 471
BspACI CCGC 5 cut(s) 68, 142, 725, 1123, 1161
BspANI GGCC 3 cut(s) 247, 255, 1164
BspCNI CTCAG 6 cut(s) 262, 421, 636, 952, 1128, 1249
BspFNI CGCG 1 cut(s) 1537
BspLI GGNNCC 2 cut(s) 367, 1459
BspMAI CTGCAG 1 cut(s) 1273
BspOI GCTAGC 1 cut(s) 704
BspPI GGATC 7 cut(s) 41, 571, 707, 857, 1321, 1452, 1465
BspQI GCTCTTC 2 cut(s) 122, 577
BspT104I TTCGAA 1 cut(s) 1428
BspTNI GGTCTC 1 cut(s) 1330
BsrI ACTGG 2 cut(s) 446, 1485
BssECI CCNNGG 5 cut(s) 269, 385, 425, 471, 790
BssHII GCGCGC 1 cut(s) 1535
BssMI GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
BssT1I CCWWGG 4 cut(s) 269, 425, 471, 790
Bst2UI CCWGG 2 cut(s) 81, 1455
Bst4CI ACNGT 6 cut(s) 131, 343, 836, 889, 1010, 1153
Bst6I CTCTTC 3 cut(s) 122, 577, 1329
BstBI TTCGAA 1 cut(s) 1428
BstC8I GCNNGC 7 cut(s) 90, 478, 482, 702, 910, 1234, 1537
BstDEI CTNAG 7 cut(s) 249, 408, 623, 939, 1115, 1257, 1301
BstDSI CCRYGG 2 cut(s) 425, 471
BstEII GGTNACC 2 cut(s) 151, 746
BstF5I GGATG 4 cut(s) 62, 442, 1166, 1537
BstFNI CGCG 1 cut(s) 1537
BstHHI GCGC 2 cut(s) 1537, 1539
BstKTI GATC 9 cut(s) 43, 49, 527, 566, 622, 715, 865, 1316, 1460
BstMAI GTCTC 2 cut(s) 332, 1330
BstMBI GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
BstMWI GCNNNNNNNGC 3 cut(s) 576, 949, 1170
BstNI CCWGG 2 cut(s) 81, 1455
BstNSI RCATGY 1 cut(s) 436
BstPI GGTNACC 2 cut(s) 151, 746
BstSCI CCNGG 2 cut(s) 79, 1453
BstSFI CTRYAG 2 cut(s) 888, 1269
BstSLI GKGCMC 1 cut(s) 670
BstUI CGCG 1 cut(s) 1537
BstV1I GCAGC 2 cut(s) 856, 1527
BstX2I RGATCY 2 cut(s) 862, 1457
BstYI RGATCY 2 cut(s) 862, 1457
Bsu36I CCTNAGG 1 cut(s) 249
BsuRI GGCC 3 cut(s) 247, 255, 1164
BtgI CCRYGG 2 cut(s) 425, 471
BtsCI GGATG 4 cut(s) 62, 442, 1166, 1537
BtsIMutI CAGTG 1 cut(s) 1478
Cac8I GCNNGC 7 cut(s) 90, 478, 482, 702, 910, 1234, 1537
CaiI CAGNNNCTG 2 cut(s) 299, 1205
CfoI GCGC 2 cut(s) 1537, 1539
Cfr13I GGNCC 2 cut(s) 253, 1163
Csp6I GTAC 5 cut(s) 405, 773, 885, 1202, 1433
CviAII CATG 4 cut(s) 308, 426, 433, 472
CviQI GTAC 5 cut(s) 405, 773, 885, 1202, 1433
DdeI CTNAG 7 cut(s) 249, 408, 623, 939, 1115, 1257, 1301
DpnI GATC 9 cut(s) 42, 48, 526, 565, 621, 714, 864, 1315, 1459
DpnII GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
DraI TTTAAA 2 cut(s) 27, 678
Eam1104I CTCTTC 3 cut(s) 122, 577, 1329
EarI CTCTTC 3 cut(s) 122, 577, 1329
Eco130I CCWWGG 4 cut(s) 269, 425, 471, 790
Eco24I GRGCYC 1 cut(s) 1287
Eco31I GGTCTC 1 cut(s) 1330
Eco32I GATATC 2 cut(s) 1022, 1298
Eco57I CTGAAG 3 cut(s) 92, 789, 1227
Eco81I CCTNAGG 1 cut(s) 249
Eco88I CYCGRG 1 cut(s) 1442
Eco91I GGTNACC 2 cut(s) 151, 746
EcoO109I RGGNCCY 1 cut(s) 253
EcoO65I GGTNACC 2 cut(s) 151, 746
EcoRII CCWGG 2 cut(s) 79, 1453
EcoRV GATATC 2 cut(s) 1022, 1298
EcoT14I CCWWGG 4 cut(s) 269, 425, 471, 790
EcoT38I GRGCYC 1 cut(s) 1287
ErhI CCWWGG 4 cut(s) 269, 425, 471, 790
FaeI CATG 4 cut(s) 311, 429, 436, 475
FalI AAGNNNNNCTT 6 cut(s) 762, 794, 927, 959, 1035, 1067
FaqI GGGAC 2 cut(s) 443, 910
FatI CATG 4 cut(s) 307, 425, 432, 471
FauI CCCGC 1 cut(s) 75
Fnu4HI GCNGC 3 cut(s) 870, 1162, 1516
FokI GGATG 3 cut(s) 49, 429, 1153
FriOI GRGCYC 1 cut(s) 1287
Fsp4HI GCNGC 3 cut(s) 870, 1162, 1516
FspBI CTAG 8 cut(s) 161, 257, 522, 701, 791, 860, 932, 1025
GlaI GCGC 2 cut(s) 1536, 1538
GluI GCNGC 3 cut(s) 870, 1162, 1516
HaeIII GGCC 3 cut(s) 247, 255, 1164
HapII CCGG 1 cut(s) 1529
HhaI GCGC 2 cut(s) 1537, 1539
Hin1II CATG 4 cut(s) 311, 429, 436, 475
Hin6I GCGC 2 cut(s) 1535, 1537
HinP1I GCGC 2 cut(s) 1535, 1537
HindIII AAGCTT 1 cut(s) 86
HinfI GANTC 3 cut(s) 19, 795, 968
HpaII CCGG 1 cut(s) 1529
HphI GGTGA 5 cut(s) 414, 548, 722, 758, 827
Hpy166II GTNNAC 1 cut(s) 1381
Hpy188I TCNGA 6 cut(s) 127, 379, 769, 949, 1019, 1118
Hpy8I GTNNAC 1 cut(s) 1381
HpyAV CCTTC 6 cut(s) 197, 247, 606, 1113, 1202, 1304
HpyCH4III ACNGT 6 cut(s) 131, 343, 836, 889, 1010, 1153
HpyCH4IV ACGT 1 cut(s) 1488
HpyCH4V TGCA 7 cut(s) 14, 92, 293, 806, 1271, 1374, 1389
HpyF10VI GCNNNNNNNGC 3 cut(s) 576, 949, 1170
HpyF3I CTNAG 7 cut(s) 249, 408, 623, 939, 1115, 1257, 1301
HpySE526I ACGT 1 cut(s) 1488
Hsp92II CATG 4 cut(s) 311, 429, 436, 475
HspAI GCGC 2 cut(s) 1535, 1537
Kzo9I GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
LguI GCTCTTC 2 cut(s) 122, 577
LmnI GCTCC 6 cut(s) 417, 496, 515, 628, 662, 1063
Lsp1109I GCAGC 2 cut(s) 856, 1527
LweI GCATC 3 cut(s) 220, 493, 1522
MaeI CTAG 8 cut(s) 161, 257, 522, 701, 791, 860, 932, 1025
MaeII ACGT 1 cut(s) 1488
MaeIII GTNAC 5 cut(s) 58, 151, 542, 746, 836
MalI GATC 9 cut(s) 42, 48, 526, 565, 621, 714, 864, 1315, 1459
MboI GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
MboII GAAGA 8 cut(s) 109, 564, 770, 993, 1052, 1316, 1406, 1481
MfeI CAATTG 1 cut(s) 93
MflI RGATCY 2 cut(s) 862, 1457
MhlI GDGCHC 3 cut(s) 501, 670, 1287
MlyI GAGTC 1 cut(s) 804
MmeI TCCRAC 1 cut(s) 1485
MroXI GAANNNNTTC 2 cut(s) 207, 774
MseI TTAA 6 cut(s) 26, 333, 677, 738, 1031, 1191
MspA1I CMGCKG 3 cut(s) 70, 869, 1133
MspI CCGG 1 cut(s) 1529
MspR9I CCNGG 2 cut(s) 81, 1455
MunI CAATTG 1 cut(s) 93
MvaI CCWGG 2 cut(s) 81, 1455
MvnI CGCG 1 cut(s) 1537
MwoI GCNNNNNNNGC 3 cut(s) 576, 949, 1170
NcoI CCATGG 2 cut(s) 425, 471
NdeII GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
NheI GCTAGC 1 cut(s) 700
NlaIII CATG 4 cut(s) 311, 429, 436, 475
NlaIV GGNNCC 2 cut(s) 367, 1459
NmuCI GTSAC 1 cut(s) 746
NspI RCATGY 1 cut(s) 436
NspV TTCGAA 1 cut(s) 1428
PaeR7I CTCGAG 1 cut(s) 1442
PauI GCGCGC 1 cut(s) 1535
PciI ACATGT 1 cut(s) 432
PciSI GCTCTTC 2 cut(s) 122, 577
PcsI WCGNNNNNNNCGW 1 cut(s) 49
PdmI GAANNNNTTC 2 cut(s) 207, 774
PfeI GAWTC 2 cut(s) 19, 968
PfoI TCCNGGA 2 cut(s) 79, 1453
PkrI GCNGC 3 cut(s) 871, 1163, 1517
PleI GAGTC 1 cut(s) 803
PpsI GAGTC 1 cut(s) 803
PscI ACATGT 1 cut(s) 432
Psp6I CCWGG 2 cut(s) 79, 1453
PspEI GGTNACC 2 cut(s) 151, 746
PspGI CCWGG 2 cut(s) 79, 1453
PspN4I GGNNCC 2 cut(s) 367, 1459
PspPI GGNCC 2 cut(s) 253, 1163
PstI CTGCAG 1 cut(s) 1273
PstNI CAGNNNCTG 2 cut(s) 299, 1205
PsuI RGATCY 2 cut(s) 862, 1457
PteI GCGCGC 1 cut(s) 1535
PvuII CAGCTG 2 cut(s) 869, 1133
RsaI GTAC 5 cut(s) 406, 774, 886, 1203, 1434
RsaNI GTAC 5 cut(s) 405, 773, 885, 1202, 1433
SapI GCTCTTC 2 cut(s) 122, 577
SaqAI TTAA 6 cut(s) 26, 333, 677, 738, 1031, 1191
SatI GCNGC 3 cut(s) 870, 1162, 1516
Sau3AI GATC 9 cut(s) 40, 46, 524, 563, 619, 712, 862, 1313, 1457
Sau96I GGNCC 2 cut(s) 253, 1163
ScaI AGTACT 3 cut(s) 406, 774, 886
SchI GAGTC 1 cut(s) 804
ScrFI CCNGG 2 cut(s) 81, 1455
SduI GDGCHC 3 cut(s) 501, 670, 1287
SfaNI GCATC 3 cut(s) 220, 493, 1522
SfcI CTRYAG 2 cut(s) 888, 1269
Sfr274I CTCGAG 1 cut(s) 1442
SfuI TTCGAA 1 cut(s) 1428
SlaI CTCGAG 1 cut(s) 1442
SmlI CTYRAG 1 cut(s) 1442
SmoI CTYRAG 1 cut(s) 1442
SsiI CCGC 5 cut(s) 68, 142, 725, 1123, 1161
SspMI CTAG 8 cut(s) 161, 257, 522, 701, 791, 860, 932, 1025
StyD4I CCNGG 2 cut(s) 79, 1453
StyI CCWWGG 4 cut(s) 269, 425, 471, 790
TaaI ACNGT 6 cut(s) 131, 343, 836, 889, 1010, 1153
TaiI ACGT 1 cut(s) 1491
TaqI TCGA 3 cut(s) 692, 1428, 1443
TatI WGTACW 3 cut(s) 404, 772, 884
TauI GCSGC 1 cut(s) 1164
TfiI GAWTC 2 cut(s) 19, 968
Tru1I TTAA 6 cut(s) 26, 333, 677, 738, 1031, 1191
Tru9I TTAA 6 cut(s) 26, 333, 677, 738, 1031, 1191
TscAI CASTG 1 cut(s) 1485
TseFI GTSAC 1 cut(s) 746
TseI GCWGC 2 cut(s) 869, 1515
Tsp45I GTSAC 1 cut(s) 746
TspDTI ATGAA 5 cut(s) 109, 210, 431, 597, 1316
TspGWI ACGGA 2 cut(s) 936, 993
TspRI CASTG 1 cut(s) 1485
XapI RAATTY 6 cut(s) 28, 169, 603, 730, 984, 1449
XbaI TCTAGA 2 cut(s) 931, 1024
XceI RCATGY 1 cut(s) 436
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XhoI CTCGAG 1 cut(s) 1442
XmaJI CCTAGG 1 cut(s) 790
XmnI GAANNNNTTC 2 cut(s) 207, 774
XspI CTAG 8 cut(s) 161, 257, 522, 701, 791, 860, 932, 1025
ZrmI AGTACT 3 cut(s) 406, 774, 886
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.