pycom12g20480

Domain of unknown function (DUF1995)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009621
Physical Location & Seq
Reverse (-)
21974523 .. 21977009
2487 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1056 bp
ATGATGGCTTCCTCCACATCCCCAAACTGCTTGTTTCACTTCAGCTCCCTTTCTTCTTCTTCTTCAGTATCCAACTCAACCACCACCACACATTTGCCCTTATCTTCTTCTCATATTATCTTCAATGGCTGCAGTGCAACTTCCCTCACCAAATCTTCTTCCTCTTCAAGAATATCTGCCAAGTTTGATAAGTTCCAAGGTGGGATTCCCCAGGAAATTCTTCAAGATGCCACACCACCACCATCTCTAGAAGCTCCTGACGAAGAAGATGGTGAGCAGGAAGATGATGACAGCTGCTTGCCCTCTAACTTGGAGGGTGCAGTGCGTCAATCAGGTGAAGCAAGTGCAGACTTTGTGTCTTCAGGAGGAATGAGAGCCATAGTTGAGCTTTTGATCCCTCAGCTGCAATTTCTAGACGAAGAAGGTGCACAAGCTGAGCTCTGGGAATTGTCGAGGGTTTTCTTAGATACGCTGATTGAAGAAACTGGCGGTCAGAGAGTTAAAGCTGTGTTTCCTGATGCTGGTGCGGCAGCGCTTCTAAAACACCGTTGGAAAGATGCTGCTTTTGGATTCGCCAGCTTAAGCGACCGAAAGCCTATAACGAGTGAAGATGAGATTGTGGTTATGGTTGTTCCTGATTACCAGATGTTGGGATACGTCGAGAAACTTGCGGCTACTCTCTCAGATGACCCGCCAAGGCCTCTCATCATGTGGAACCCCCGTCTCATCAGCGAGGATGTAGGAGTTGGATTCAATGTTCGAAAGTTAAGGCGGTACTTTCTCAGAACTTTTACGACGGTCTACTCAATGAGGCCTATGGAATCTGGTGCTGTATTTAGGCGTTATCCAGGGCTATGGAAGGTGTTCTACGATGACAAGGATAGACCGAACAGATACCTGCTCGCGAAAGAGCTCATTCAGCGCCCCGACGCTGAAGAACTTGAGATGATATTCGGAAATGTAGACGAGAGCTCAGAGAAGGGCCAATCTTTGTTTGATCAAGCTGCAGGGATGTTCTCTTCGCTAAATCGATTTATGAGGGTCATTTCAAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1995

Protein Analysis

352

Amino Acids

38.93

Weight (kDa)

4.82

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1995 PF09353 101 - 315 4.3e-52 Domain of unknown function (DUF1995)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 906
AccB7I CCANNNNNTGG 1 cut(s) 649
AccI GTMKAC 2 cut(s) 801, 963
AccII CGCG 1 cut(s) 905
AciI CCGC 5 cut(s) 489, 527, 671, 692, 772
AclWI GGATC 1 cut(s) 388
AcsI RAATTY 1 cut(s) 216
AcuI CTGAAG 4 cut(s) 25, 48, 345, 954
AfaI GTAC 1 cut(s) 776
AfeI AGCGCT 1 cut(s) 534
AfiI CCNNNNNNNGG 2 cut(s) 521, 649
AflII CTTAAG 1 cut(s) 580
AgsI TTSAA 6 cut(s) 124, 168, 224, 479, 754, 1050
AhdI GACNNNNNGTC 1 cut(s) 355
AjnI CCWGG 2 cut(s) 210, 847
AjuI GAANNNNNNNTTGG 2 cut(s) 189, 221
AloI GAACNNNNNNTCC 2 cut(s) 741, 773
Alw21I GWGCWC 4 cut(s) 430, 441, 915, 974
Alw26I GTCTC 1 cut(s) 728
Alw44I GTGCAC 1 cut(s) 426
AlwI GGATC 1 cut(s) 388
Aor51HI AGCGCT 1 cut(s) 534
AoxI GGCC 3 cut(s) 698, 812, 982
ApaLI GTGCAC 1 cut(s) 426
ApeKI GCWGC 6 cut(s) 129, 294, 403, 530, 560, 1004
ApoI RAATTY 1 cut(s) 216
Asp700I GAANNNNTTC 2 cut(s) 219, 863
AspLEI GCGC 2 cut(s) 535, 924
AspS9I GGNCC 1 cut(s) 982
AsuHPI GGTGA 3 cut(s) 139, 284, 347
AsuII TTCGAA 1 cut(s) 760
BaeGI GKGCMC 1 cut(s) 430
BanII GRGCYC 3 cut(s) 441, 915, 974
BarI GAAGNNNNNNTAC 2 cut(s) 851, 883
BbsI GAAGAC 1 cut(s) 351
Bbv12I GWGCWC 4 cut(s) 430, 441, 915, 974
BbvCI CCTCAGC 1 cut(s) 399
BbvI GCAGC 6 cut(s) 116, 281, 390, 542, 547, 991
BccI CCATC 2 cut(s) 250, 263
BcgI CGANNNNNNTGC 4 cut(s) 407, 441, 650, 684
BciT130I CCWGG 2 cut(s) 212, 849
BciVI GTATCC 2 cut(s) 79, 647
BclI TGATCA 1 cut(s) 997
BcoDI GTCTC 1 cut(s) 728
BfaI CTAG 2 cut(s) 248, 413
BfmI CTRYAG 2 cut(s) 130, 1005
BfoI RGCGCY 2 cut(s) 536, 925
BfrI CTTAAG 1 cut(s) 580
BfuAI ACCTGC 1 cut(s) 906
BfuI GTATCC 2 cut(s) 79, 647
BisI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
BlpI GCTNAGC 1 cut(s) 435
BlsI GCNGC 8 cut(s) 131, 296, 405, 529, 532, 562, 673, 1006
Bme1390I CCNGG 2 cut(s) 212, 849
BmeRI GACNNNNNGTC 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 982
BmiI GGNNCC 1 cut(s) 716
BmrFI CCNGG 2 cut(s) 212, 849
BmsI GCATC 3 cut(s) 217, 508, 547
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 399
Bpu1102I GCTNAGC 1 cut(s) 435
Bpu14I TTCGAA 1 cut(s) 760
BpuEI CTTGAG 1 cut(s) 962
Bsa29I ATCGAT 1 cut(s) 1030
BsaJI CCNNGG 4 cut(s) 196, 210, 695, 848
BsaXI ACNNNNNCTCC 2 cut(s) 29, 59
Bsc4I CCNNNNNNNGG 2 cut(s) 521, 649
Bse1I ACTGG 1 cut(s) 490
BseBI CCWGG 2 cut(s) 212, 849
BseCI ATCGAT 1 cut(s) 1030
BseDI CCNNGG 4 cut(s) 196, 210, 695, 848
BseGI GGATG 3 cut(s) 17, 742, 1017
BseLI CCNNNNNNNGG 2 cut(s) 521, 649
BseMII CTCAG 5 cut(s) 413, 426, 696, 796, 987
BseNI ACTGG 1 cut(s) 490
BseSI GKGCMC 1 cut(s) 430
BseXI GCAGC 6 cut(s) 116, 281, 390, 542, 547, 991
BsgI GTGCAG 2 cut(s) 339, 366
Bsh1236I CGCG 1 cut(s) 905
Bsh1285I CGRYCG 1 cut(s) 589
BshFI GGCC 3 cut(s) 700, 814, 984
BshVI ATCGAT 1 cut(s) 1030
BsiEI CGRYCG 1 cut(s) 589
BsiHKAI GWGCWC 4 cut(s) 430, 441, 915, 974
BslI CCNNNNNNNGG 2 cut(s) 521, 649
BsmAI GTCTC 1 cut(s) 728
BsmBI CGTCTC 1 cut(s) 728
BsnI GGCC 3 cut(s) 700, 814, 984
Bsp119I TTCGAA 1 cut(s) 760
Bsp1286I GDGCHC 4 cut(s) 430, 441, 915, 974
Bsp143I GATC 2 cut(s) 393, 997
Bsp1720I GCTNAGC 1 cut(s) 435
Bsp68I TCGCGA 1 cut(s) 905
BspACI CCGC 5 cut(s) 489, 527, 671, 692, 772
BspANI GGCC 3 cut(s) 700, 814, 984
BspCNI CTCAG 5 cut(s) 412, 427, 695, 795, 986
BspDI ATCGAT 1 cut(s) 1030
BspFNI CGCG 1 cut(s) 905
BspLI GGNNCC 1 cut(s) 716
BspMAI CTGCAG 2 cut(s) 134, 1009
BspMI ACCTGC 1 cut(s) 906
BspPI GGATC 1 cut(s) 388
BspT104I TTCGAA 1 cut(s) 760
BspTI CTTAAG 1 cut(s) 580
BsrI ACTGG 1 cut(s) 490
BssECI CCNNGG 4 cut(s) 196, 210, 695, 848
BssMI GATC 2 cut(s) 393, 997
BssT1I CCWWGG 2 cut(s) 196, 695
Bst2UI CCWGG 2 cut(s) 212, 849
Bst4CI ACNGT 2 cut(s) 548, 799
Bst6I CTCTTC 2 cut(s) 169, 1024
BstAFI CTTAAG 1 cut(s) 580
BstBI TTCGAA 1 cut(s) 760
BstC8I GCNNGC 3 cut(s) 299, 577, 903
BstDEI CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
BstF5I GGATG 3 cut(s) 17, 742, 1017
BstFNI CGCG 1 cut(s) 905
BstH2I RGCGCY 2 cut(s) 536, 925
BstHHI GCGC 2 cut(s) 535, 924
BstKTI GATC 2 cut(s) 396, 1000
BstMAI GTCTC 1 cut(s) 728
BstMBI GATC 2 cut(s) 393, 997
BstMCI CGRYCG 1 cut(s) 589
BstMWI GCNNNNNNNGC 2 cut(s) 527, 919
BstNI CCWGG 2 cut(s) 212, 849
BstSCI CCNGG 2 cut(s) 210, 847
BstSFI CTRYAG 2 cut(s) 130, 1005
BstSLI GKGCMC 1 cut(s) 430
BstUI CGCG 1 cut(s) 905
BstV1I GCAGC 6 cut(s) 116, 281, 390, 542, 547, 991
BstV2I GAAGAC 1 cut(s) 351
BstXI CCANNNNNNTGG 1 cut(s) 855
Bsu15I ATCGAT 1 cut(s) 1030
BsuI GTATCC 2 cut(s) 79, 647
BsuRI GGCC 3 cut(s) 700, 814, 984
BsuTUI ATCGAT 1 cut(s) 1030
BtsCI GGATG 3 cut(s) 17, 742, 1017
BtsI GCAGTG 2 cut(s) 139, 327
BtsIMutI CAGTG 2 cut(s) 139, 327
BtuMI TCGCGA 1 cut(s) 905
BveI ACCTGC 1 cut(s) 906
Cac8I GCNNGC 3 cut(s) 299, 577, 903
CfoI GCGC 2 cut(s) 535, 924
Cfr13I GGNCC 1 cut(s) 982
ClaI ATCGAT 1 cut(s) 1030
CseI GACGC 2 cut(s) 314, 938
Csp6I GTAC 1 cut(s) 775
CviAII CATG 1 cut(s) 709
CviQI GTAC 1 cut(s) 775
DdeI CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
DpnI GATC 2 cut(s) 395, 999
DpnII GATC 2 cut(s) 393, 997
DriI GACNNNNNGTC 1 cut(s) 355
Eam1104I CTCTTC 2 cut(s) 169, 1024
Eam1105I GACNNNNNGTC 1 cut(s) 355
EarI CTCTTC 2 cut(s) 169, 1024
Ecl136II GAGCTC 3 cut(s) 439, 913, 972
Eco130I CCWWGG 2 cut(s) 196, 695
Eco147I AGGCCT 2 cut(s) 700, 814
Eco24I GRGCYC 3 cut(s) 441, 915, 974
Eco47III AGCGCT 1 cut(s) 534
Eco53kI GAGCTC 3 cut(s) 439, 913, 972
Eco57I CTGAAG 4 cut(s) 25, 48, 345, 954
EcoICRI GAGCTC 3 cut(s) 439, 913, 972
EcoRII CCWGG 2 cut(s) 210, 847
EcoT14I CCWWGG 2 cut(s) 196, 695
EcoT38I GRGCYC 3 cut(s) 441, 915, 974
ErhI CCWWGG 2 cut(s) 196, 695
Esp3I CGTCTC 1 cut(s) 728
FaeI CATG 1 cut(s) 712
FaiI YATR 8 cut(s) 114, 380, 599, 626, 710, 818, 856, 1037
FatI CATG 1 cut(s) 708
FauI CCCGC 1 cut(s) 699
FbaI TGATCA 1 cut(s) 997
FblI GTMKAC 2 cut(s) 801, 963
Fnu4HI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
FokI GGATG 3 cut(s) 4, 749, 1024
FriOI GRGCYC 3 cut(s) 441, 915, 974
Fsp4HI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
FspBI CTAG 2 cut(s) 248, 413
GlaI GCGC 2 cut(s) 534, 923
GluI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
HaeII RGCGCY 2 cut(s) 536, 925
HaeIII GGCC 3 cut(s) 700, 814, 984
HgaI GACGC 2 cut(s) 314, 938
HhaI GCGC 2 cut(s) 535, 924
Hin1II CATG 1 cut(s) 712
Hin6I GCGC 2 cut(s) 533, 922
HinP1I GCGC 2 cut(s) 533, 922
HinfI GANTC 4 cut(s) 205, 570, 750, 821
HphI GGTGA 3 cut(s) 139, 284, 347
Hpy166II GTNNAC 3 cut(s) 428, 802, 964
Hpy188I TCNGA 5 cut(s) 495, 685, 785, 956, 976
Hpy8I GTNNAC 3 cut(s) 428, 802, 964
Hpy99I CGWCG 3 cut(s) 662, 799, 932
HpyAV CCTTC 3 cut(s) 416, 853, 973
HpyCH4III ACNGT 2 cut(s) 548, 799
HpyCH4IV ACGT 1 cut(s) 657
HpyCH4V TGCA 7 cut(s) 132, 137, 320, 347, 406, 428, 1007
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 919
HpyF3I CTNAG 6 cut(s) 399, 435, 463, 682, 782, 973
HpySE526I ACGT 1 cut(s) 657
Hsp92II CATG 1 cut(s) 712
HspAI GCGC 2 cut(s) 533, 922
Ksp22I TGATCA 1 cut(s) 997
Kzo9I GATC 2 cut(s) 393, 997
LmnI GCTCC 2 cut(s) 50, 259
Lsp1109I GCAGC 6 cut(s) 116, 281, 390, 542, 547, 991
LweI GCATC 3 cut(s) 217, 508, 547
MaeI CTAG 2 cut(s) 248, 413
MaeII ACGT 1 cut(s) 657
MalI GATC 2 cut(s) 395, 999
MboI GATC 2 cut(s) 393, 997
MhlI GDGCHC 4 cut(s) 430, 441, 915, 974
MluCI AATT 3 cut(s) 216, 407, 446
MmeI TCCRAC 3 cut(s) 96, 530, 727
MroXI GAANNNNTTC 2 cut(s) 219, 863
MseI TTAA 3 cut(s) 501, 581, 767
MspA1I CMGCKG 2 cut(s) 294, 403
MspCI CTTAAG 1 cut(s) 580
MspR9I CCNGG 2 cut(s) 212, 849
MvaI CCWGG 2 cut(s) 212, 849
MvnI CGCG 1 cut(s) 905
MwoI GCNNNNNNNGC 2 cut(s) 527, 919
NdeII GATC 2 cut(s) 393, 997
NlaIII CATG 1 cut(s) 712
NlaIV GGNNCC 1 cut(s) 716
NruI TCGCGA 1 cut(s) 905
NspV TTCGAA 1 cut(s) 760
PceI AGGCCT 2 cut(s) 700, 814
PdmI GAANNNNTTC 2 cut(s) 219, 863
PfeI GAWTC 4 cut(s) 205, 570, 750, 821
PflMI CCANNNNNTGG 1 cut(s) 649
PkrI GCNGC 8 cut(s) 131, 296, 405, 529, 532, 562, 673, 1006
Psp124BI GAGCTC 3 cut(s) 441, 915, 974
Psp6I CCWGG 2 cut(s) 210, 847
PspGI CCWGG 2 cut(s) 210, 847
PspN4I GGNNCC 1 cut(s) 716
PspPI GGNCC 1 cut(s) 982
PstI CTGCAG 2 cut(s) 134, 1009
PvuII CAGCTG 2 cut(s) 294, 403
RruI TCGCGA 1 cut(s) 905
RsaI GTAC 1 cut(s) 776
RsaNI GTAC 1 cut(s) 775
SacI GAGCTC 3 cut(s) 441, 915, 974
SaqAI TTAA 3 cut(s) 501, 581, 767
SatI GCNGC 8 cut(s) 130, 295, 404, 528, 531, 561, 672, 1005
Sau3AI GATC 2 cut(s) 393, 997
Sau96I GGNCC 1 cut(s) 982
ScrFI CCNGG 2 cut(s) 212, 849
SduI GDGCHC 4 cut(s) 430, 441, 915, 974
SfaNI GCATC 3 cut(s) 217, 508, 547
SfcI CTRYAG 2 cut(s) 130, 1005
SfuI TTCGAA 1 cut(s) 760
SmlI CTYRAG 2 cut(s) 580, 941
SmoI CTYRAG 2 cut(s) 580, 941
Sse9I AATT 3 cut(s) 216, 407, 446
SseBI AGGCCT 2 cut(s) 700, 814
SsiI CCGC 5 cut(s) 489, 527, 671, 692, 772
SspMI CTAG 2 cut(s) 248, 413
SstI GAGCTC 3 cut(s) 441, 915, 974
StuI AGGCCT 2 cut(s) 700, 814
StyD4I CCNGG 2 cut(s) 210, 847
StyI CCWWGG 2 cut(s) 196, 695
TaaI ACNGT 2 cut(s) 548, 799
TaiI ACGT 1 cut(s) 660
TaqI TCGA 4 cut(s) 452, 660, 760, 1030
TaqII GACCGA 2 cut(s) 603, 901
TasI AATT 3 cut(s) 216, 407, 446
TauI GCSGC 2 cut(s) 530, 674
TfiI GAWTC 4 cut(s) 205, 570, 750, 821
Tru1I TTAA 3 cut(s) 501, 581, 767
Tru9I TTAA 3 cut(s) 501, 581, 767
TscAI CASTG 2 cut(s) 139, 327
TseI GCWGC 6 cut(s) 129, 294, 403, 530, 560, 1004
TspRI CASTG 2 cut(s) 139, 327
Van91I CCANNNNNTGG 1 cut(s) 649
Vha464I CTTAAG 1 cut(s) 580
VneI GTGCAC 1 cut(s) 426
XapI RAATTY 1 cut(s) 216
XbaI TCTAGA 2 cut(s) 247, 412
XmiI GTMKAC 2 cut(s) 801, 963
XmnI GAANNNNTTC 2 cut(s) 219, 863
XspI CTAG 2 cut(s) 248, 413
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.