pycom12g20520

Belongs to the glycosyltransferase 8 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0009206
Physical Location & Seq
Reverse (-)
21996375 .. 21998555
2181 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1338 bp
ATGGCTTCCAAGTTTTCTTCCTCCTCCTCCTCCTCCTCCTATGCCAAACGACGAGTAGTCCCCTCCGTTCTCTATCTAATTATTTTCTCTCTATTCTTCCTATGCCTAATTATCTCGTTTAACTTTCGTCCCACCAAGTCAAATAATTGCGCTACCATTCGAAACCAACAGGTTATCAAGAATGAAACCAAGAACGAAAGAAAGAACGTAGATTGGTTCGTTGCCATTTCCAAGCTATTCAAAATCAAGGGTAGAAAAATTAAGGTAGGTTTGGTCAACGTAGATGAACACATTCATAGTCAGCTCCATGGACTATCGGATGTAGAGGCAGTCTCAGTGGCATTCGAACGCGTAGCTAGCGATCGGAAATGGAAGGACTATTTTCCCGAATGGATAGATGAAGACGAGAAATGGGGGAAGCCGAAGTGTCCGGAAATCCCAATTCCTAAGCTGGAGCATTACGAGGGCATAGATGTGGTACTGGCCAAGGTTCCCTGTGGACCAAATGGGACGAGTGACAGGGAAGGGATTAGAGATGTGTTTAGGTTACAAGTTAACTTGATGGTGGCTAATCTAGTGGTGGGTAAAGGGTGGATGAAGCCCGATGCTCATCGGACGGTGTACGTTGTGTTTATCGGGGATTGTGGACCGATGGTGGAGATTTTCAGATGTGATGATCTTTTGATGCATAGAGGGAATTATTGGGTTTATAGGCCTAATATTGGGAGATTGAAACAAATTGTGCATATGCCTGTTGGATCTTGCCAAGTTGCCTCAGGTTATGCGGAAACAGGCAGAGAAATCTGGAGAAAATTCATGTTAGAAACAAGCTCATGGATTCAATACGAGGGACGTAGACTTGCCTATGTGACAATCCTGCACTCCTCAGAAGCCTATGTGTGTGGTGCAATAGCCCTAGCTCGAAGCATCAGGCAAACCAACTCCACCAAAGACCTAGTCCTCCTTGCAGATGACTTCATCACTCCCAAATCCATCCAAGGCCTAACCGCGGCAGGATGGAAGATCAAGCGAATCCAGAGAATCCGAAGTGCATTTGCTAAAAAAGGGTCGTACAACGAATGGAACTACAGCAAACTCCGCGTGTGGCAGCTCACGGAGTATGACAAGGTTATTTTCATTGACGCGGATCTTCTAGTCCTCAAGAACATAGATAGTTTCTTCGTGCACCAACAGTTGTCAGCCGTGGGGAATAACCGGGTGTTGTTCAACTCCGGCTTGATGGTTGTGGAGCCATCGAACTGCATGTTCGAATACCTAACGCAAAAAACATCCAAACTCGAGTCTTACAACGGCGGCGATCGATCCAGGGGTTTTTGA

Protein Analysis

446

Amino Acids

50.9

Weight (kDa)

9.38

Isoelectric Point (pI)

30.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_transf_8 PF01501 292 - 394 2.1e-10 Glycosyl transferase family 8
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 856
AccII CGCG 4 cut(s) 351, 1010, 1101, 1145
AccIII TCCGGA 1 cut(s) 430
AciI CCGC 6 cut(s) 785, 1008, 1010, 1099, 1145, 1314
AclWI GGATC 3 cut(s) 766, 1155, 1317
AcoI YGGCCR 1 cut(s) 483
AcsI RAATTY 1 cut(s) 812
AfaI GTAC 3 cut(s) 480, 623, 1073
AfiI CCNNNNNNNGG 2 cut(s) 722, 1009
AflIII ACRYGT 1 cut(s) 349
AgsI TTSAA 4 cut(s) 241, 733, 842, 1228
AhdI GACNNNNNGTC 1 cut(s) 56
AjnI CCWGG 1 cut(s) 1325
AluBI AGCT 7 cut(s) 235, 304, 356, 451, 831, 920, 1111
AluI AGCT 7 cut(s) 235, 304, 356, 451, 831, 920, 1111
Alw21I GWGCWC 1 cut(s) 1188
Alw26I GTCTC 1 cut(s) 337
Alw44I GTGCAC 1 cut(s) 1184
AlwI GGATC 3 cut(s) 766, 1155, 1317
Ama87I CYCGRG 1 cut(s) 1298
Aor13HI TCCGGA 1 cut(s) 430
AoxI GGCC 3 cut(s) 483, 713, 1000
ApaLI GTGCAC 1 cut(s) 1184
ApeKI GCWGC 1 cut(s) 1108
ApoI RAATTY 1 cut(s) 812
ArsI GACNNNNNNTTYG 2 cut(s) 942, 974
Asp700I GAANNNNTTC 1 cut(s) 291
AspLEI GCGC 1 cut(s) 152
AspS9I GGNCC 2 cut(s) 500, 647
AsuC2I CCSGG 1 cut(s) 1217
AsuII TTCGAA 3 cut(s) 160, 345, 1269
AsuNHI GCTAGC 1 cut(s) 356
AvaI CYCGRG 1 cut(s) 1298
AvaII GGWCC 2 cut(s) 500, 647
AxyI CCTNAGG 1 cut(s) 775
BaeGI GKGCMC 1 cut(s) 1188
BalI TGGCCA 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 408
Bbv12I GWGCWC 1 cut(s) 1188
BbvI GCAGC 1 cut(s) 1120
BccI CCATC 6 cut(s) 556, 646, 1001, 1011, 1234, 1261
BceAI ACGGC 2 cut(s) 1187, 1327
BciT130I CCWGG 1 cut(s) 1327
BcnI CCSGG 1 cut(s) 1217
BcoDI GTCTC 1 cut(s) 337
BfaI CTAG 5 cut(s) 357, 575, 917, 956, 1154
BfmI CTRYAG 1 cut(s) 1087
BisI GCNGC 3 cut(s) 1011, 1109, 1315
BlsI GCNGC 3 cut(s) 1012, 1110, 1316
Bme1390I CCNGG 2 cut(s) 1217, 1327
Bme18I GGWCC 2 cut(s) 500, 647
BmeRI GACNNNNNGTC 1 cut(s) 56
BmeT110I CYCGRG 1 cut(s) 1298
BmgT120I GGNCC 2 cut(s) 500, 647
BmiI GGNNCC 2 cut(s) 492, 1251
BmrFI CCNGG 2 cut(s) 1217, 1327
BmsI GCATC 3 cut(s) 595, 675, 936
BmtI GCTAGC 1 cut(s) 360
BpiI GAAGAC 1 cut(s) 408
BplI GAGNNNNNCTC 2 cut(s) 317, 349
BpmI CTGGAG 2 cut(s) 473, 826
Bpu10I CCTNAGC 1 cut(s) 447
Bpu14I TTCGAA 3 cut(s) 160, 345, 1269
BpuEI CTTGAG 1 cut(s) 1145
BpuMI CCSGG 1 cut(s) 1217
Bsa29I ATCGAT 1 cut(s) 1321
BsaBI GATNNNNATC 1 cut(s) 609
BsaJI CCNNGG 6 cut(s) 307, 486, 997, 1008, 1203, 1326
BsaWI WCCGGW 1 cut(s) 430
Bsc4I CCNNNNNNNGG 2 cut(s) 722, 1009
Bse1I ACTGG 1 cut(s) 486
Bse21I CCTNAGG 1 cut(s) 775
Bse8I GATNNNNATC 1 cut(s) 609
BseAI TCCGGA 1 cut(s) 430
BseBI CCWGG 1 cut(s) 1327
BseCI ATCGAT 1 cut(s) 1321
BseDI CCNNGG 6 cut(s) 307, 486, 997, 1008, 1203, 1326
BseGI GGATG 5 cut(s) 325, 600, 993, 1022, 1289
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 2 cut(s) 722, 1009
BseMII CTCAG 3 cut(s) 348, 789, 900
BseNI ACTGG 1 cut(s) 486
BseRI GAGGAG 6 cut(s) 13, 16, 19, 22, 25, 874
BseSI GKGCMC 1 cut(s) 1188
BseXI GCAGC 1 cut(s) 1120
BsgI GTGCAG 1 cut(s) 863
Bsh1236I CGCG 4 cut(s) 351, 1010, 1101, 1145
Bsh1285I CGRYCG 2 cut(s) 364, 1321
BshFI GGCC 3 cut(s) 485, 715, 1002
BshVI ATCGAT 1 cut(s) 1321
BsiEI CGRYCG 2 cut(s) 364, 1321
BsiHKAI GWGCWC 1 cut(s) 1188
BsiHKCI CYCGRG 1 cut(s) 1298
BsiSI CCGG 3 cut(s) 431, 1216, 1233
BslFI GGGAC 4 cut(s) 44, 114, 523, 864
BslI CCNNNNNNNGG 2 cut(s) 722, 1009
BsmAI GTCTC 1 cut(s) 337
BsmFI GGGAC 4 cut(s) 44, 114, 523, 864
BsmI GAATGC 1 cut(s) 341
BsnI GGCC 3 cut(s) 485, 715, 1002
BsoBI CYCGRG 1 cut(s) 1298
Bsp119I TTCGAA 3 cut(s) 160, 345, 1269
Bsp1286I GDGCHC 1 cut(s) 1188
Bsp13I TCCGGA 1 cut(s) 430
Bsp143I GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
Bsp19I CCATGG 1 cut(s) 307
BspACI CCGC 6 cut(s) 785, 1008, 1010, 1099, 1145, 1314
BspANI GGCC 3 cut(s) 485, 715, 1002
BspCNI CTCAG 3 cut(s) 347, 788, 899
BspDI ATCGAT 1 cut(s) 1321
BspEI TCCGGA 1 cut(s) 430
BspFNI CGCG 4 cut(s) 351, 1010, 1101, 1145
BspLI GGNNCC 2 cut(s) 492, 1251
BspOI GCTAGC 1 cut(s) 360
BspPI GGATC 3 cut(s) 766, 1155, 1317
BspT104I TTCGAA 3 cut(s) 160, 345, 1269
BsrI ACTGG 1 cut(s) 486
BssECI CCNNGG 6 cut(s) 307, 486, 997, 1008, 1203, 1326
BssMI GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
BssT1I CCWWGG 3 cut(s) 307, 486, 997
Bst2UI CCWGG 1 cut(s) 1327
Bst4CI ACNGT 2 cut(s) 619, 1194
BstBI TTCGAA 3 cut(s) 160, 345, 1269
BstC8I GCNNGC 1 cut(s) 358
BstDEI CTNAG 4 cut(s) 334, 447, 775, 886
BstDSI CCRYGG 3 cut(s) 307, 1008, 1203
BstF5I GGATG 5 cut(s) 325, 600, 993, 1022, 1289
BstFNI CGCG 4 cut(s) 351, 1010, 1101, 1145
BstHHI GCGC 1 cut(s) 152
BstKTI GATC 7 cut(s) 364, 679, 761, 1026, 1150, 1321, 1325
BstMAI GTCTC 1 cut(s) 337
BstMBI GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
BstMCI CGRYCG 2 cut(s) 364, 1321
BstMWI GCNNNNNNNGC 2 cut(s) 357, 1098
BstNI CCWGG 1 cut(s) 1327
BstNSI RCATGY 1 cut(s) 1267
BstSCI CCNGG 2 cut(s) 1215, 1325
BstSFI CTRYAG 1 cut(s) 1087
BstSLI GKGCMC 1 cut(s) 1188
BstUI CGCG 4 cut(s) 351, 1010, 1101, 1145
BstV1I GCAGC 1 cut(s) 1120
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 2 cut(s) 758, 1147
BstYI RGATCY 2 cut(s) 758, 1147
Bsu15I ATCGAT 1 cut(s) 1321
Bsu36I CCTNAGG 1 cut(s) 775
BsuRI GGCC 3 cut(s) 485, 715, 1002
BsuTUI ATCGAT 1 cut(s) 1321
BtgI CCRYGG 3 cut(s) 307, 1008, 1203
BtsCI GGATG 5 cut(s) 325, 600, 993, 1022, 1289
BtsIMutI CAGTG 1 cut(s) 342
Cac8I GCNNGC 1 cut(s) 358
CfoI GCGC 1 cut(s) 152
Cfr13I GGNCC 2 cut(s) 500, 647
Cfr42I CCGCGG 1 cut(s) 1011
ClaI ATCGAT 1 cut(s) 1321
CseI GACGC 1 cut(s) 1151
Csp6I GTAC 3 cut(s) 479, 622, 1072
CviAII CATG 4 cut(s) 308, 817, 834, 1264
CviQI GTAC 3 cut(s) 479, 622, 1072
DdeI CTNAG 4 cut(s) 334, 447, 775, 886
DpnI GATC 7 cut(s) 363, 678, 760, 1025, 1149, 1320, 1324
DpnII GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
DriI GACNNNNNGTC 1 cut(s) 56
EaeI YGGCCR 1 cut(s) 483
Eam1105I GACNNNNNGTC 1 cut(s) 56
Eco130I CCWWGG 3 cut(s) 307, 486, 997
Eco147I AGGCCT 2 cut(s) 715, 1002
Eco47I GGWCC 2 cut(s) 500, 647
Eco81I CCTNAGG 1 cut(s) 775
Eco88I CYCGRG 1 cut(s) 1298
EcoRII CCWGG 1 cut(s) 1325
EcoT14I CCWWGG 3 cut(s) 307, 486, 997
EcoT22I ATGCAT 1 cut(s) 690
ErhI CCWWGG 3 cut(s) 307, 486, 997
FaeI CATG 4 cut(s) 311, 820, 837, 1267
FaqI GGGAC 4 cut(s) 44, 114, 523, 864
FatI CATG 4 cut(s) 307, 816, 833, 1263
FauNDI CATATG 1 cut(s) 747
FblI GTMKAC 1 cut(s) 856
Fnu4HI GCNGC 3 cut(s) 1011, 1109, 1315
FokI GGATG 5 cut(s) 332, 607, 980, 1029, 1276
Fsp4HI GCNGC 3 cut(s) 1011, 1109, 1315
FspBI CTAG 5 cut(s) 357, 575, 917, 956, 1154
GlaI GCGC 1 cut(s) 151
GluI GCNGC 3 cut(s) 1011, 1109, 1315
GsuI CTGGAG 2 cut(s) 473, 826
HaeIII GGCC 3 cut(s) 485, 715, 1002
HapII CCGG 3 cut(s) 431, 1216, 1233
HgaI GACGC 1 cut(s) 1151
HhaI GCGC 1 cut(s) 152
Hin1II CATG 4 cut(s) 311, 820, 837, 1267
Hin6I GCGC 1 cut(s) 150
HinP1I GCGC 1 cut(s) 150
HincII GTYRAC 2 cut(s) 277, 556
HindII GTYRAC 2 cut(s) 277, 556
HinfI GANTC 4 cut(s) 838, 1032, 1041, 1301
HpaI GTTAAC 1 cut(s) 556
HpaII CCGG 3 cut(s) 431, 1216, 1233
Hpy166II GTNNAC 7 cut(s) 277, 500, 556, 622, 647, 857, 1186
Hpy188I TCNGA 6 cut(s) 319, 366, 615, 668, 889, 1046
Hpy188III TCNNGA 6 cut(s) 178, 386, 431, 805, 1036, 1162
Hpy8I GTNNAC 7 cut(s) 277, 500, 556, 622, 647, 857, 1186
Hpy99I CGWCG 1 cut(s) 54
HpyAV CCTTC 2 cut(s) 367, 518
HpyCH4III ACNGT 2 cut(s) 619, 1194
HpyCH4IV ACGT 4 cut(s) 207, 279, 624, 853
HpyCH4V TGCA 8 cut(s) 688, 745, 880, 908, 968, 1052, 1186, 1263
HpyF10VI GCNNNNNNNGC 2 cut(s) 357, 1098
HpyF3I CTNAG 4 cut(s) 334, 447, 775, 886
HpySE526I ACGT 4 cut(s) 207, 279, 624, 853
Hsp92II CATG 4 cut(s) 311, 820, 837, 1267
HspAI GCGC 1 cut(s) 150
Kpn2I TCCGGA 1 cut(s) 430
KspAI GTTAAC 1 cut(s) 556
KspI CCGCGG 1 cut(s) 1011
Kzo9I GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
LmnI GCTCC 3 cut(s) 309, 454, 1249
Lsp1109I GCAGC 1 cut(s) 1120
LweI GCATC 3 cut(s) 595, 675, 936
MaeI CTAG 5 cut(s) 357, 575, 917, 956, 1154
MaeII ACGT 4 cut(s) 207, 279, 624, 853
MaeIII GTNAC 3 cut(s) 515, 546, 868
MalI GATC 7 cut(s) 363, 678, 760, 1025, 1149, 1320, 1324
MboI GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
MboII GAAGA 6 cut(s) 9, 88, 413, 1033, 1142, 1171
MflI RGATCY 2 cut(s) 758, 1147
MhlI GDGCHC 1 cut(s) 1188
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 8 cut(s) 78, 108, 145, 258, 441, 697, 738, 812
MluI ACGCGT 1 cut(s) 349
MluNI TGGCCA 1 cut(s) 485
MlyI GAGTC 1 cut(s) 1310
MmeI TCCRAC 1 cut(s) 736
Mox20I TGGCCA 1 cut(s) 485
Mph1103I ATGCAT 1 cut(s) 690
MroI TCCGGA 1 cut(s) 430
MroXI GAANNNNTTC 1 cut(s) 291
MscI TGGCCA 1 cut(s) 485
MseI TTAA 3 cut(s) 120, 261, 555
MslI CAYNNNNRTG 1 cut(s) 473
Msp20I TGGCCA 1 cut(s) 485
MspA1I CMGCKG 1 cut(s) 1010
MspI CCGG 3 cut(s) 431, 1216, 1233
MspR9I CCNGG 2 cut(s) 1217, 1327
Mva1269I GAATGC 1 cut(s) 341
MvaI CCWGG 1 cut(s) 1327
MvnI CGCG 4 cut(s) 351, 1010, 1101, 1145
MwoI GCNNNNNNNGC 2 cut(s) 357, 1098
NciI CCSGG 1 cut(s) 1217
NcoI CCATGG 1 cut(s) 307
NdeI CATATG 1 cut(s) 747
NdeII GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
NheI GCTAGC 1 cut(s) 356
NlaIII CATG 4 cut(s) 311, 820, 837, 1267
NlaIV GGNNCC 2 cut(s) 492, 1251
NmuCI GTSAC 2 cut(s) 515, 868
NsiI ATGCAT 1 cut(s) 690
NspI RCATGY 1 cut(s) 1267
NspV TTCGAA 3 cut(s) 160, 345, 1269
PaeR7I CTCGAG 1 cut(s) 1298
PceI AGGCCT 2 cut(s) 715, 1002
PctI GAATGC 1 cut(s) 341
PdmI GAANNNNTTC 1 cut(s) 291
PfeI GAWTC 3 cut(s) 838, 1032, 1041
PflFI GACNNNGTC 1 cut(s) 956
PkrI GCNGC 3 cut(s) 1012, 1110, 1316
Ple19I CGATCG 2 cut(s) 364, 1321
PleI GAGTC 1 cut(s) 1309
PpsI GAGTC 1 cut(s) 1309
Psp6I CCWGG 1 cut(s) 1325
PspGI CCWGG 1 cut(s) 1325
PspN4I GGNNCC 2 cut(s) 492, 1251
PspPI GGNCC 2 cut(s) 500, 647
PspXI VCTCGAGB 1 cut(s) 1298
PsuI RGATCY 2 cut(s) 758, 1147
PsyI GACNNNGTC 1 cut(s) 956
PvuI CGATCG 2 cut(s) 364, 1321
RsaI GTAC 3 cut(s) 480, 623, 1073
RsaNI GTAC 3 cut(s) 479, 622, 1072
RseI CAYNNNNRTG 1 cut(s) 473
SacII CCGCGG 1 cut(s) 1011
SaqAI TTAA 3 cut(s) 120, 261, 555
SatI GCNGC 3 cut(s) 1011, 1109, 1315
Sau3AI GATC 7 cut(s) 361, 676, 758, 1023, 1147, 1318, 1322
Sau96I GGNCC 2 cut(s) 500, 647
SchI GAGTC 1 cut(s) 1310
ScrFI CCNGG 2 cut(s) 1217, 1327
SduI GDGCHC 1 cut(s) 1188
SfaNI GCATC 3 cut(s) 595, 675, 936
SfcI CTRYAG 1 cut(s) 1087
Sfr274I CTCGAG 1 cut(s) 1298
Sfr303I CCGCGG 1 cut(s) 1011
SfuI TTCGAA 3 cut(s) 160, 345, 1269
SgrBI CCGCGG 1 cut(s) 1011
SinI GGWCC 2 cut(s) 500, 647
SlaI CTCGAG 1 cut(s) 1298
SmiMI CAYNNNNRTG 1 cut(s) 473
SmlI CTYRAG 2 cut(s) 1160, 1298
SmoI CTYRAG 2 cut(s) 1160, 1298
Sse9I AATT 8 cut(s) 78, 108, 145, 258, 441, 697, 738, 812
SseBI AGGCCT 2 cut(s) 715, 1002
SsiI CCGC 6 cut(s) 785, 1008, 1010, 1099, 1145, 1314
SspI AATATT 1 cut(s) 721
SspMI CTAG 5 cut(s) 357, 575, 917, 956, 1154
StuI AGGCCT 2 cut(s) 715, 1002
StyD4I CCNGG 2 cut(s) 1215, 1325
StyI CCWWGG 3 cut(s) 307, 486, 997
TaaI ACNGT 2 cut(s) 619, 1194
TaiI ACGT 4 cut(s) 210, 282, 627, 856
TaqI TCGA 7 cut(s) 160, 345, 922, 1256, 1269, 1299, 1321
TaqII GACCGA 1 cut(s) 664
TasI AATT 8 cut(s) 78, 108, 145, 258, 441, 697, 738, 812
TauI GCSGC 2 cut(s) 1013, 1317
TfiI GAWTC 3 cut(s) 838, 1032, 1041
Tru1I TTAA 3 cut(s) 120, 261, 555
Tru9I TTAA 3 cut(s) 120, 261, 555
TscAI CASTG 1 cut(s) 342
TseFI GTSAC 2 cut(s) 515, 868
TseI GCWGC 1 cut(s) 1108
Tsp45I GTSAC 2 cut(s) 515, 868
TspDTI ATGAA 8 cut(s) 198, 284, 300, 414, 611, 805, 967, 1126
TspGWI ACGGA 2 cut(s) 55, 1130
TspRI CASTG 1 cut(s) 342
Tth111I GACNNNGTC 1 cut(s) 956
VneI GTGCAC 1 cut(s) 1184
VpaK11BI GGWCC 2 cut(s) 500, 647
XapI RAATTY 1 cut(s) 812
XceI RCATGY 1 cut(s) 1267
XhoI CTCGAG 1 cut(s) 1298
XmiI GTMKAC 1 cut(s) 856
XmnI GAANNNNTTC 1 cut(s) 291
XspI CTAG 5 cut(s) 357, 575, 917, 956, 1154
Zsp2I ATGCAT 1 cut(s) 690
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.