pycom12g21440

FRIGIDA-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0012633
Physical Location & Seq
Forward (+)
22611696 .. 22614259
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2019 bp
ATGTCGACCACCGTCGACAAAATTTCCGGTGACCTTCACATGAGGCCGGAGCTACGCAAATCCTTCAACCTCCTCAAGACCCACGCCTCCAAAGTTGCCAACTTTACCCTCCAATGGCAGGACCTCGAGGAGCACTTCCGCTCCATCGACAATTCCATCCAATCCAAGCTCGACCAACTGCAAAAACAACAATCGGAAGAGACCCATCTCGCTCCGGCTCCGCAATCGCAACCCAGAGAACCCGAATTGAATTTTAAACCCAATTCCGAAGAACCCCAATTGGGTTCCGGTGACGCCCAATTGAGTTCGAAAGAATCCCAAGTCACCGCCACCCTCCAGTCCTTTAAAACCCAATTCGATTCCGTCGAAATCCAAGTCAAAACCGACGAGCCTTTGCCAGAAATGTCGACCTGCAATGGCGTCCCGATAAATGACGGCAAGGCGTTGCTGTTGTACGTGAATGAGCATTTGAAAGAGCGGGGTTCGGTGCGTGACGGTGTTGCGGATGCGCTTAAGGTTTCTGTGGATGCTGGGAGGCTAGTGCTCGAGGCAGTGAAATGGTTTTATCCATCAGAATCGAATAAGGGAGGTAAGGAGCTTGACGTCACTGTTACTAGGAAAAGTTGTGCGATTTTGTTACAAGGGCTGGTGAATGTGAAACCAGTGATTAAACCCGATATCATCAGAGAGGCAATGAAGCTTGCATTGGCTTGGAAGGCAAAGGTGATAACTCAAATGTCCAACTCTTTGGAGGTTTGGGCTTTTTTTCAGCTTCTGGATGCTTTTGGGTTGGGTGGTGAGTTTGACAGTGATGAGATTTTGAAGCTTTTTAGTTGCATAGCAGATCGGAATTCTGCACCGGAGTTGTTTAGGTCTCTTGGTTTCGCCGATAAAGCCTCTGATTTCATCCAAAAGCTTGTATCAGAGAATAAGCGGCTTGACGCTGTTAGATTCAGTCATGCATTTGGACAGTTTGACAAGTTCCCCCCTGTACCCTTGCTTAAAGCACATTTAAAGTTCATCACGAAAGATGCAAGGACAAGTTCCAAGAGGGGGCAAGACCGTCTTAAAGCACAGGATGAGGCCATAGACAAAGAAATAGCTGCTCTAAGAGGCATAATACGTTGCGTTGAGGACTACAAGCTTGTCGTTGAACCAGAATATTCTCCTGAGATCCTTGGAAAACACATTAGGCAGCTCAGAAGGGAGAGAAAAGAGAGGAAAGCAACTCTAACAGCTCCTGGCCCTAAGGCTCAGCCGCAACCCCTGAGTGGTAAGAAACGTACTGCCCCAGGTCAGCCAAATAAGCAGAACAGAAATAAGCATCCTCGAACAGCTCGAGCTTTAACTGCTCAAACTGTTTCTTTCAGAGGACCAGCCACAGTACGCGCTATACAACCATCTTATATTCGCCAAGTAAGCTTGTATGACAGTCGAAGTGCAGAATACTTGTTATCGTCGGCTGGAATGCCCAGACAAGCAACCACTCCGCCCAGTACTTCTTATGGCACCATTTCCACCTTGAACCCACTGCGAGCATCTCATCATGAGCAAGGAGGCTCATTCATTGGTCAAGGTGCAGAATACCCTTCTCGGTACTATGATTCAGCACACTACGCTCCTGATACTAATAATAACTTATCTGCTCGATCGTATGTCTTGGTGGGTTCCTCTCCTGGTACCCGTGGCCAGCTCGTGAACTTAACAGCTCAAGACTATGGATTGACAGGTCCGTCTCATGTTCATACTAACTCAGAACTTGGGGGCAGTGGTCATGTAACTTCAGATCTGAGCCCATTAGTCGCACGATACAGAACAAGTATAGCTGCAAATGGAAGGACTGGACAGTTAGGATCAGCAGGAAGCCCTACACCAGTGCGCGTTGCAAGCCCGAACAGTTCCATCCCTTATGCCTATAGGGATCCACTTAGGGCGCCTAACTACAATGACCGGGCAGTGCGTTCCAAGAGTGGCTATGGCGCGCCATCTCAGCACCCCCCAGCCATTTATCACCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Frigida

Protein Analysis

673

Amino Acids

74.03

Weight (kDa)

9.42

Isoelectric Point (pI)

48.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Frigida PF07899 145 - 410 3.8e-71 Frigida-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1732
AatII GACGTC 1 cut(s) 606
AbsI CCTCGAGG 1 cut(s) 125
Acc36I ACCTGC 1 cut(s) 419
Acc65I GGTACC 1 cut(s) 1679
AccB1I GGYRCC 3 cut(s) 1508, 1679, 1933
AccBSI CCGCTC 2 cut(s) 141, 478
AccI GTMKAC 3 cut(s) 5, 15, 407
AccII CGCG 3 cut(s) 1389, 1881, 1982
AciI CCGC 8 cut(s) 139, 221, 327, 478, 503, 934, 1259, 1490
AclWI GGATC 4 cut(s) 1168, 1861, 1916, 1929
AcoI YGGCCR 1 cut(s) 1687
AcsI RAATTY 3 cut(s) 21, 250, 850
AcuI CTGAAG 1 cut(s) 1767
AcyI GRCGYC 4 cut(s) 294, 420, 603, 1934
AfaI GTAC 7 cut(s) 455, 993, 1285, 1386, 1498, 1598, 1681
AfiI CCNNNNNNNGG 5 cut(s) 114, 118, 281, 1053, 1271
AflII CTTAAG 1 cut(s) 512
AgsI TTSAA 6 cut(s) 67, 250, 472, 823, 1154, 1525
AjnI CCWGG 3 cut(s) 1240, 1291, 1675
AjuI GAANNNNNNNTTGG 2 cut(s) 689, 721
Alw21I GWGCWC 2 cut(s) 135, 546
Alw26I GTCTC 3 cut(s) 194, 879, 1740
AlwI GGATC 4 cut(s) 1168, 1861, 1916, 1929
AlwNI CAGNNNCTG 2 cut(s) 775, 1241
Ama87I CYCGRG 3 cut(s) 125, 545, 1338
AoxI GGCC 4 cut(s) 44, 1083, 1243, 1687
ApeKI GCWGC 3 cut(s) 1103, 1195, 1826
ApoI RAATTY 3 cut(s) 21, 250, 850
ArsI GACNNNNNNTTYG 2 cut(s) 360, 392
AscI GGCGCGCC 1 cut(s) 1980
Asp718I GGTACC 1 cut(s) 1679
AspLEI GCGC 6 cut(s) 511, 1391, 1881, 1936, 1982, 1984
AspS9I GGNCC 4 cut(s) 121, 1244, 1373, 1730
AsuC2I CCSGG 1 cut(s) 1952
AsuHPI GGTGA 7 cut(s) 41, 302, 316, 661, 736, 809, 2003
AsuII TTCGAA 1 cut(s) 308
AvaI CYCGRG 3 cut(s) 125, 545, 1338
AvaII GGWCC 3 cut(s) 121, 1373, 1730
AxyI CCTNAGG 1 cut(s) 1248
BalI TGGCCA 1 cut(s) 1689
BamHI GGATCC 1 cut(s) 1921
BanI GGYRCC 3 cut(s) 1508, 1679, 1933
BanII GRGCYC 1 cut(s) 1796
BauI CACGAG 1 cut(s) 1694
Bbv12I GWGCWC 2 cut(s) 135, 546
BbvI GCAGC 3 cut(s) 1090, 1207, 1813
BccI CCATC 7 cut(s) 152, 164, 213, 577, 1408, 1910, 1993
BceAI ACGGC 1 cut(s) 451
BciT130I CCWGG 3 cut(s) 1242, 1293, 1677
BcnI CCSGG 1 cut(s) 1952
BcoDI GTCTC 3 cut(s) 194, 879, 1740
BfaI CTAG 2 cut(s) 539, 615
BfmI CTRYAG 1 cut(s) 1915
BfoI RGCGCY 1 cut(s) 1937
BfrI CTTAAG 1 cut(s) 512
BfuAI ACCTGC 1 cut(s) 419
BglII AGATCT 1 cut(s) 1786
BisI GCNGC 5 cut(s) 935, 1104, 1196, 1259, 1827
BlpI GCTNAGC 1 cut(s) 1254
BlsI GCNGC 5 cut(s) 936, 1105, 1197, 1260, 1828
BmcAI AGTACT 1 cut(s) 1498
Bme1390I CCNGG 4 cut(s) 1242, 1293, 1677, 1952
Bme18I GGWCC 3 cut(s) 121, 1373, 1730
BmeT110I CYCGRG 3 cut(s) 125, 545, 1338
BmgT120I GGNCC 4 cut(s) 121, 1244, 1373, 1730
BmiI GGNNCC 7 cut(s) 219, 286, 1510, 1669, 1681, 1923, 1935
BmrFI CCNGG 4 cut(s) 1242, 1293, 1677, 1952
BmrI ACTGGG 1 cut(s) 1488
BmsI GCATC 6 cut(s) 496, 517, 769, 1021, 1333, 1547
BmuI ACTGGG 1 cut(s) 1488
BoxI GACNNNNGTC 1 cut(s) 11
BpmI CTGGAG 1 cut(s) 320
Bpu1102I GCTNAGC 1 cut(s) 1254
Bpu14I TTCGAA 1 cut(s) 308
BpuEI CTTGAG 2 cut(s) 59, 1695
BpuMI CCSGG 1 cut(s) 1952
BsaAI YACGTR 1 cut(s) 457
BsaHI GRCGYC 4 cut(s) 294, 420, 603, 1934
BsaI GGTCTC 2 cut(s) 194, 879
BsaJI CCNNGG 3 cut(s) 1177, 1291, 1684
BsaWI WCCGGW 3 cut(s) 26, 287, 859
BsaXI ACNNNNNCTCC 2 cut(s) 125, 155
Bsc4I CCNNNNNNNGG 5 cut(s) 114, 118, 281, 1053, 1271
Bse1I ACTGG 5 cut(s) 337, 662, 1494, 1846, 1874
Bse21I CCTNAGG 1 cut(s) 1248
Bse3DI GCAATG 2 cut(s) 421, 699
BseBI CCWGG 3 cut(s) 1242, 1293, 1677
BseDI CCNNGG 3 cut(s) 1177, 1291, 1684
BseGI GGATG 8 cut(s) 156, 511, 532, 784, 906, 1084, 1324, 1902
BseLI CCNNNNNNNGG 5 cut(s) 114, 118, 281, 1053, 1271
BseMI GCAATG 2 cut(s) 421, 699
BseMII CTCAG 7 cut(s) 1161, 1213, 1259, 1268, 1767, 1781, 2003
BseNI ACTGG 5 cut(s) 337, 662, 1494, 1846, 1874
BsePI GCGCGC 1 cut(s) 1980
BseRI GAGGAG 2 cut(s) 62, 143
BseXI GCAGC 3 cut(s) 1090, 1207, 1813
BseYI CCCAGC 2 cut(s) 530, 1999
BsgI GTGCAG 3 cut(s) 840, 1461, 1599
Bsh1236I CGCG 3 cut(s) 1389, 1881, 1982
Bsh1285I CGRYCG 1 cut(s) 1652
BshFI GGCC 4 cut(s) 46, 1085, 1245, 1689
BshNI GGYRCC 3 cut(s) 1508, 1679, 1933
BsiEI CGRYCG 1 cut(s) 1652
BsiHKAI GWGCWC 2 cut(s) 135, 546
BsiHKCI CYCGRG 3 cut(s) 125, 545, 1338
BsiSI CCGG 6 cut(s) 27, 47, 215, 288, 860, 1951
BslFI GGGAC 1 cut(s) 407
BslI CCNNNNNNNGG 5 cut(s) 114, 118, 281, 1053, 1271
BsmAI GTCTC 3 cut(s) 194, 879, 1740
BsmBI CGTCTC 1 cut(s) 1740
BsmFI GGGAC 1 cut(s) 407
BsmI GAATGC 1 cut(s) 1473
BsnI GGCC 4 cut(s) 46, 1085, 1245, 1689
Bso31I GGTCTC 2 cut(s) 194, 879
BsoBI CYCGRG 3 cut(s) 125, 545, 1338
Bsp119I TTCGAA 1 cut(s) 308
Bsp1286I GDGCHC 3 cut(s) 135, 546, 1796
Bsp143I GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
Bsp1720I GCTNAGC 1 cut(s) 1254
BspACI CCGC 8 cut(s) 139, 221, 327, 478, 503, 934, 1259, 1490
BspANI GGCC 4 cut(s) 46, 1085, 1245, 1689
BspCNI CTCAG 7 cut(s) 1162, 1212, 1260, 1267, 1766, 1782, 2002
BspFNI CGCG 3 cut(s) 1389, 1881, 1982
BspHI TCATGA 1 cut(s) 1546
BspLI GGNNCC 7 cut(s) 219, 286, 1510, 1669, 1681, 1923, 1935
BspMI ACCTGC 1 cut(s) 419
BspPI GGATC 4 cut(s) 1168, 1861, 1916, 1929
BspT104I TTCGAA 1 cut(s) 308
BspT107I GGYRCC 3 cut(s) 1508, 1679, 1933
BspTI CTTAAG 1 cut(s) 512
BspTNI GGTCTC 2 cut(s) 194, 879
BsrBI CCGCTC 2 cut(s) 141, 478
BsrDI GCAATG 2 cut(s) 421, 699
BsrI ACTGG 5 cut(s) 337, 662, 1494, 1846, 1874
BssECI CCNNGG 3 cut(s) 1177, 1291, 1684
BssHII GCGCGC 1 cut(s) 1980
BssMI GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
BssNI GRCGYC 4 cut(s) 294, 420, 603, 1934
BssSI CACGAG 1 cut(s) 1694
BssT1I CCWWGG 1 cut(s) 1177
Bst2BI CACGAG 1 cut(s) 1694
Bst2UI CCWGG 3 cut(s) 1242, 1293, 1677
Bst6I CTCTTC 1 cut(s) 192
BstACI GRCGYC 4 cut(s) 294, 420, 603, 1934
BstAFI CTTAAG 1 cut(s) 512
BstBAI YACGTR 1 cut(s) 457
BstBI TTCGAA 1 cut(s) 308
BstC8I GCNNGC 5 cut(s) 702, 1536, 1691, 1888, 1982
BstDSI CCRYGG 1 cut(s) 1684
BstEII GGTNACC 1 cut(s) 29
BstF5I GGATG 8 cut(s) 156, 511, 532, 784, 906, 1084, 1324, 1902
BstFNI CGCG 3 cut(s) 1389, 1881, 1982
BstH2I RGCGCY 1 cut(s) 1937
BstHHI GCGC 6 cut(s) 511, 1391, 1881, 1936, 1982, 1984
BstKTI GATC 6 cut(s) 847, 1176, 1652, 1789, 1856, 1924
BstMAI GTCTC 3 cut(s) 194, 879, 1740
BstMBI GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
BstMCI CGRYCG 1 cut(s) 1652
BstMWI GCNNNNNNNGC 8 cut(s) 716, 893, 1306, 1349, 1419, 1616, 1887, 1990
BstNI CCWGG 3 cut(s) 1242, 1293, 1677
BstPAI GACNNNNGTC 1 cut(s) 11
BstPI GGTNACC 1 cut(s) 29
BstSCI CCNGG 4 cut(s) 1240, 1291, 1675, 1950
BstSFI CTRYAG 1 cut(s) 1915
BstUI CGCG 3 cut(s) 1389, 1881, 1982
BstV1I GCAGC 3 cut(s) 1090, 1207, 1813
BstX2I RGATCY 3 cut(s) 1173, 1786, 1921
BstXI CCANNNNNNTGG 1 cut(s) 748
BstYI RGATCY 3 cut(s) 1173, 1786, 1921
Bsu36I CCTNAGG 1 cut(s) 1248
BsuRI GGCC 4 cut(s) 46, 1085, 1245, 1689
BtgI CCRYGG 1 cut(s) 1684
BtsCI GGATG 8 cut(s) 156, 511, 532, 784, 906, 1084, 1324, 1902
BtsI GCAGTG 4 cut(s) 558, 1529, 1774, 1962
BtsIMutI CAGTG 8 cut(s) 558, 606, 669, 814, 1529, 1774, 1881, 1962
BveI ACCTGC 1 cut(s) 419
Cac8I GCNNGC 5 cut(s) 702, 1536, 1691, 1888, 1982
CaiI CAGNNNCTG 2 cut(s) 775, 1241
CciI TCATGA 1 cut(s) 1546
CfoI GCGC 6 cut(s) 511, 1391, 1881, 1936, 1982, 1984
Cfr13I GGNCC 4 cut(s) 121, 1244, 1373, 1730
CseI GACGC 3 cut(s) 302, 409, 950
Csp6I GTAC 7 cut(s) 454, 992, 1284, 1385, 1497, 1597, 1680
CviAII CATG 5 cut(s) 40, 959, 1547, 1739, 1775
CviQI GTAC 7 cut(s) 454, 992, 1284, 1385, 1497, 1597, 1680
DinI GGCGCC 1 cut(s) 1935
DpnI GATC 6 cut(s) 846, 1175, 1651, 1788, 1855, 1923
DpnII GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
DraI TTTAAA 3 cut(s) 256, 346, 1014
DrdI GACNNNNNNGTC 1 cut(s) 1732
DseDI GACNNNNNNGTC 1 cut(s) 1732
EaeI YGGCCR 1 cut(s) 1687
Eam1104I CTCTTC 1 cut(s) 192
EarI CTCTTC 1 cut(s) 192
EciI GGCGGA 1 cut(s) 1479
Eco130I CCWWGG 1 cut(s) 1177
Eco24I GRGCYC 1 cut(s) 1796
Eco31I GGTCTC 2 cut(s) 194, 879
Eco32I GATATC 1 cut(s) 679
Eco47I GGWCC 3 cut(s) 121, 1373, 1730
Eco57I CTGAAG 1 cut(s) 1767
Eco81I CCTNAGG 1 cut(s) 1248
Eco88I CYCGRG 3 cut(s) 125, 545, 1338
Eco91I GGTNACC 1 cut(s) 29
EcoO109I RGGNCCY 1 cut(s) 121
EcoO65I GGTNACC 1 cut(s) 29
EcoRI GAATTC 1 cut(s) 850
EcoRII CCWGG 3 cut(s) 1240, 1291, 1675
EcoRV GATATC 1 cut(s) 679
EcoT14I CCWWGG 1 cut(s) 1177
EcoT22I ATGCAT 1 cut(s) 964
EcoT38I GRGCYC 1 cut(s) 1796
EgeI GGCGCC 1 cut(s) 1935
EheI GGCGCC 1 cut(s) 1935
ErhI CCWWGG 1 cut(s) 1177
Esp3I CGTCTC 1 cut(s) 1740
FaeI CATG 5 cut(s) 43, 962, 1550, 1742, 1778
FalI AAGNNNNNCTT 2 cut(s) 1050, 1082
FaqI GGGAC 1 cut(s) 407
FatI CATG 5 cut(s) 39, 958, 1546, 1738, 1774
FauI CCCGC 1 cut(s) 471
FblI GTMKAC 3 cut(s) 5, 15, 407
Fnu4HI GCNGC 5 cut(s) 935, 1104, 1196, 1259, 1827
FokI GGATG 8 cut(s) 143, 518, 539, 791, 893, 1091, 1311, 1889
FriOI GRGCYC 1 cut(s) 1796
Fsp4HI GCNGC 5 cut(s) 935, 1104, 1196, 1259, 1827
FspBI CTAG 2 cut(s) 539, 615
GlaI GCGC 6 cut(s) 510, 1390, 1880, 1935, 1981, 1983
GluI GCNGC 5 cut(s) 935, 1104, 1196, 1259, 1827
GsaI CCCAGC 2 cut(s) 534, 2003
GsuI CTGGAG 1 cut(s) 320
HaeII RGCGCY 1 cut(s) 1937
HaeIII GGCC 4 cut(s) 46, 1085, 1245, 1689
HapII CCGG 6 cut(s) 27, 47, 215, 288, 860, 1951
HgaI GACGC 3 cut(s) 302, 409, 950
HhaI GCGC 6 cut(s) 511, 1391, 1881, 1936, 1982, 1984
Hin1I GRCGYC 4 cut(s) 294, 420, 603, 1934
Hin1II CATG 5 cut(s) 43, 962, 1550, 1742, 1778
Hin6I GCGC 6 cut(s) 509, 1389, 1879, 1934, 1980, 1982
HinP1I GCGC 6 cut(s) 509, 1389, 1879, 1934, 1980, 1982
HincII GTYRAC 3 cut(s) 6, 16, 408
HindII GTYRAC 3 cut(s) 6, 16, 408
HindIII AAGCTT 5 cut(s) 698, 824, 914, 1142, 1420
HinfI GANTC 5 cut(s) 314, 359, 575, 951, 1604
HpaII CCGG 6 cut(s) 27, 47, 215, 288, 860, 1951
HphI GGTGA 7 cut(s) 41, 302, 316, 661, 736, 809, 2003
Hpy166II GTNNAC 4 cut(s) 6, 16, 408, 1699
Hpy188III TCNNGA 9 cut(s) 76, 424, 776, 1024, 1169, 1547, 1622, 1696, 1712
Hpy8I GTNNAC 4 cut(s) 6, 16, 408, 1699
Hpy99I CGWCG 4 cut(s) 17, 368, 389, 1462
HpyAV CCTTC 6 cut(s) 44, 73, 709, 1197, 1599, 1830
HpyCH4IV ACGT 4 cut(s) 456, 603, 1123, 1282
HpyF10VI GCNNNNNNNGC 8 cut(s) 716, 893, 1306, 1349, 1419, 1616, 1887, 1990
HpySE526I ACGT 4 cut(s) 456, 603, 1123, 1282
Hsp92I GRCGYC 4 cut(s) 294, 420, 603, 1934
Hsp92II CATG 5 cut(s) 43, 962, 1550, 1742, 1778
HspAI GCGC 6 cut(s) 509, 1389, 1879, 1934, 1980, 1982
KasI GGCGCC 1 cut(s) 1933
KpnI GGTACC 1 cut(s) 1683
Kzo9I GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
LmnI GCTCC 8 cut(s) 49, 130, 146, 217, 223, 595, 1243, 1624
Lsp1109I GCAGC 3 cut(s) 1090, 1207, 1813
LweI GCATC 6 cut(s) 496, 517, 769, 1021, 1333, 1547
MaeI CTAG 2 cut(s) 539, 615
MaeII ACGT 4 cut(s) 456, 603, 1123, 1282
MaeIII GTNAC 8 cut(s) 29, 290, 322, 491, 604, 610, 636, 1777
MalI GATC 6 cut(s) 846, 1175, 1651, 1788, 1855, 1923
MbiI CCGCTC 2 cut(s) 141, 478
MboI GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
MboII GAAGA 2 cut(s) 209, 281
MfeI CAATTG 2 cut(s) 278, 299
MflI RGATCY 3 cut(s) 1173, 1786, 1921
MhlI GDGCHC 3 cut(s) 135, 546, 1796
MlsI TGGCCA 1 cut(s) 1689
MluCI AATT 9 cut(s) 21, 151, 245, 250, 262, 278, 299, 353, 850
MluNI TGGCCA 1 cut(s) 1689
Mly113I GGCGCC 1 cut(s) 1934
MmeI TCCRAC 1 cut(s) 765
Mox20I TGGCCA 1 cut(s) 1689
Mph1103I ATGCAT 1 cut(s) 964
MscI TGGCCA 1 cut(s) 1689
MseI TTAA 9 cut(s) 255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
Msp20I TGGCCA 1 cut(s) 1689
MspCI CTTAAG 1 cut(s) 512
MspI CCGG 6 cut(s) 27, 47, 215, 288, 860, 1951
MspR9I CCNGG 4 cut(s) 1242, 1293, 1677, 1952
MunI CAATTG 2 cut(s) 278, 299
Mva1269I GAATGC 1 cut(s) 1473
MvaI CCWGG 3 cut(s) 1242, 1293, 1677
MvnI CGCG 3 cut(s) 1389, 1881, 1982
MwoI GCNNNNNNNGC 8 cut(s) 716, 893, 1306, 1349, 1419, 1616, 1887, 1990
NarI GGCGCC 1 cut(s) 1934
NciI CCSGG 1 cut(s) 1952
NdeII GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
NlaIII CATG 5 cut(s) 43, 962, 1550, 1742, 1778
NlaIV GGNNCC 7 cut(s) 219, 286, 1510, 1669, 1681, 1923, 1935
NmuCI GTSAC 5 cut(s) 29, 290, 322, 491, 604
NsiI ATGCAT 1 cut(s) 964
NspV TTCGAA 1 cut(s) 308
PaeR7I CTCGAG 3 cut(s) 125, 545, 1338
PagI TCATGA 1 cut(s) 1546
PalAI GGCGCGCC 1 cut(s) 1980
PauI GCGCGC 1 cut(s) 1980
PcsI WCGNNNNNNNCGW 2 cut(s) 363, 1336
PctI GAATGC 1 cut(s) 1473
PfeI GAWTC 5 cut(s) 314, 359, 575, 951, 1604
PkrI GCNGC 5 cut(s) 936, 1105, 1197, 1260, 1828
Ple19I CGATCG 1 cut(s) 1652
PluTI GGCGCC 1 cut(s) 1937
Ppu21I YACGTR 1 cut(s) 457
PpuMI RGGWCCY 1 cut(s) 121
PshAI GACNNNNGTC 1 cut(s) 11
Psp5II RGGWCCY 1 cut(s) 121
Psp6I CCWGG 3 cut(s) 1240, 1291, 1675
PspEI GGTNACC 1 cut(s) 29
PspFI CCCAGC 2 cut(s) 530, 1999
PspGI CCWGG 3 cut(s) 1240, 1291, 1675
PspN4I GGNNCC 7 cut(s) 219, 286, 1510, 1669, 1681, 1923, 1935
PspPI GGNCC 4 cut(s) 121, 1244, 1373, 1730
PspPPI RGGWCCY 1 cut(s) 121
PspXI VCTCGAGB 3 cut(s) 125, 545, 1338
PstNI CAGNNNCTG 2 cut(s) 775, 1241
PsuI RGATCY 3 cut(s) 1173, 1786, 1921
PteI GCGCGC 1 cut(s) 1980
PvuI CGATCG 1 cut(s) 1652
RsaI GTAC 7 cut(s) 455, 993, 1285, 1386, 1498, 1598, 1681
RsaNI GTAC 7 cut(s) 454, 992, 1284, 1385, 1497, 1597, 1680
SalI GTCGAC 3 cut(s) 4, 14, 406
SaqAI TTAA 9 cut(s) 255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
SatI GCNGC 5 cut(s) 935, 1104, 1196, 1259, 1827
Sau3AI GATC 6 cut(s) 844, 1173, 1649, 1786, 1853, 1921
Sau96I GGNCC 4 cut(s) 121, 1244, 1373, 1730
ScaI AGTACT 1 cut(s) 1498
ScrFI CCNGG 4 cut(s) 1242, 1293, 1677, 1952
SduI GDGCHC 3 cut(s) 135, 546, 1796
SfaNI GCATC 6 cut(s) 496, 517, 769, 1021, 1333, 1547
SfcI CTRYAG 1 cut(s) 1915
SfoI GGCGCC 1 cut(s) 1935
Sfr274I CTCGAG 3 cut(s) 125, 545, 1338
SfuI TTCGAA 1 cut(s) 308
SgsI GGCGCGCC 1 cut(s) 1980
SinI GGWCC 3 cut(s) 121, 1373, 1730
SlaI CTCGAG 3 cut(s) 125, 545, 1338
SmlI CTYRAG 6 cut(s) 74, 125, 512, 545, 1338, 1710
SmoI CTYRAG 6 cut(s) 74, 125, 512, 545, 1338, 1710
Sse9I AATT 9 cut(s) 21, 151, 245, 250, 262, 278, 299, 353, 850
SsiI CCGC 8 cut(s) 139, 221, 327, 478, 503, 934, 1259, 1490
SspDI GGCGCC 1 cut(s) 1933
SspI AATATT 1 cut(s) 1163
SspMI CTAG 2 cut(s) 539, 615
StyD4I CCNGG 4 cut(s) 1240, 1291, 1675, 1950
StyI CCWWGG 1 cut(s) 1177
TaiI ACGT 4 cut(s) 459, 606, 1126, 1285
TasI AATT 9 cut(s) 21, 151, 245, 250, 262, 278, 299, 353, 850
TatI WGTACW 1 cut(s) 1496
TauI GCSGC 2 cut(s) 937, 1261
TfiI GAWTC 5 cut(s) 314, 359, 575, 951, 1604
Tru1I TTAA 9 cut(s) 255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
Tru9I TTAA 9 cut(s) 255, 345, 513, 669, 1002, 1013, 1068, 1346, 1703
TscAI CASTG 8 cut(s) 558, 613, 669, 814, 1536, 1774, 1881, 1962
TseFI GTSAC 5 cut(s) 29, 290, 322, 491, 604
TseI GCWGC 3 cut(s) 1103, 1195, 1826
Tsp45I GTSAC 5 cut(s) 29, 290, 322, 491, 604
TspDTI ATGAA 5 cut(s) 710, 895, 1009, 1555, 1733
TspGWI ACGGA 2 cut(s) 352, 1722
TspRI CASTG 8 cut(s) 558, 613, 669, 814, 1536, 1774, 1881, 1962
Vha464I CTTAAG 1 cut(s) 512
VpaK11BI GGWCC 3 cut(s) 121, 1373, 1730
XapI RAATTY 3 cut(s) 21, 250, 850
XhoI CTCGAG 3 cut(s) 125, 545, 1338
XmiI GTMKAC 3 cut(s) 5, 15, 407
XspI CTAG 2 cut(s) 539, 615
ZraI GACGTC 1 cut(s) 604
ZrmI AGTACT 1 cut(s) 1498
Zsp2I ATGCAT 1 cut(s) 964
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.