pycom12g22450

f-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0007822
Physical Location & Seq
Reverse (-)
23334838 .. 23337393
2556 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1401 bp
ATGAACTATTGCAAACCTTTGTTAAAAGGTGTGGCCCAAAACCCCCAACCAGTGAGATCAGAAGGCGACAGATTCTCCTATGCAACTGTGAGAGTCCAAACCTCACTCGCCGGAGCTCCTCCTCCTCCTCCGCCCTCGCGGCTCCCGGAATTCATCAAGACGCTCAAGCTCTCAGATGATATTATGACGGGTCAAAAGAGGTCTGCAAATGATGCTCATGAAGTATTCACCTGTGATGATGACATCCTACATGAGATCCTACTCCGTCTGCCAGAGAAATCTGTTTTCAGTTTAATACTCGTATCGAAGAGGTGGCTTCGTGTGATATGTAGCCCTTCTTTTCGATGTGATTACCATGCTCGATGGAAAGTAAATTATCATCTTTTGGGCTTCTTTGTGTGCAACCATCTTTACCTCGGAATATCTAAATATGGCATGCGGCGGCCCTTAACAGAGCCCGCAATTCATTTTTTGTCAGCCTGTGAGGAAGGTGATAATCTAATGGTTTCTGCACCCAAACAGCTTGGTTACTTTCTTGGCACTTCCAATGGCCTTCTTCTTTTTGGCCGCCATCCAATGACATATACGGTGTGGAACCCAGTCAGCAAACAGCTATGCAAACTTCCTCAACCTCAGCAGTTCTATCAAAGTTTGTGCATGGCATTCCTCACTGTCGAAGAGGACTGTTTTAATGATGCAGTCCACTACAAGGTTATTCGGGCAAAATGTAACTGCAGACGGGAATCTAAAACTGTCTCCATTGAGACTTACTCTTCAATCACCGGTACATGGAAGCATTTCACTCTTACTAGCTCTTCAACATTTGCATTGCGTCCTTGGAGAGAGGCTACTGTAGTTAATGGTGTCCTACACTGGTTTGCAACCCAGGGAAACCTAGCCATTTATGATCCACGTCTTGGAAATAGGCGTATAAATCTGCTTAAATTACCAGAAGGGAGGATTTCTCAAGATTATGATGAGTGCGTTCTTGGGCAGTCTTCAGATGGGCTTTTGCAGTATGGTCAGAGCAGTACATCTGGCGTGGAAATATGGGTTCTCGAGGGCGGAGAAAGTAGTTTCTCATCGATCAGCACAGGCAATGTGCAATCACAGAAGAAGTGGAATTTGAAGTACAGACTAGGTTTCAAGGCGATGTGGAAGCAGAATCCAGACCATGGAAAGCATTCGAAAGAAGCTAAACTTATGGCATTCCTTCATCAGAATTCCGAGTATATCTTCATAAGAGCTGGATGGAACATATATTTCTTTCACCTGAAAAGCCAGAAGCTTAAAGAGATTCGATATCTTGGTCGTGACCCTTCGATTCAATGGGATGACAGCACAGTGGTGCCTTACTATAGACCGTCTTGGCCACGCTCTTCTTTATGTCCCTGGATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
F-box

Protein Analysis

467

Amino Acids

53.46

Weight (kDa)

9.22

Isoelectric Point (pI)

55.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 80 - 115 2.4e-06 F-box domain
FBA_3 PF08268 170 - 384 6.1e-06 F-box associated beta propeller domain
b-prop_At3g26010-like PF24750 177 - 340 5.8e-14 F-box protein At3g26010-like, beta-propeller
FBA_1 PF07734 182 - 354 3e-10 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1348
AccB7I CCANNNNNTGG 2 cut(s) 917, 1175
AccII CGCG 1 cut(s) 139
AciI CCGC 7 cut(s) 131, 139, 439, 442, 459, 568, 1065
AclWI GGATC 2 cut(s) 250, 902
AcoI YGGCCR 2 cut(s) 565, 1370
AcsI RAATTY 3 cut(s) 149, 1123, 1222
AcuI CTGAAG 1 cut(s) 984
AfaI GTAC 3 cut(s) 787, 1033, 1133
AfiI CCNNNNNNNGG 4 cut(s) 709, 789, 917, 1175
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 5 cut(s) 777, 819, 1129, 1147, 1328
AjiI CACGTC 1 cut(s) 914
AjnI CCWGG 2 cut(s) 885, 1391
AleI CACNNNNGTG 1 cut(s) 1346
AluBI AGCT 8 cut(s) 116, 169, 523, 613, 813, 1196, 1247, 1288
AluI AGCT 8 cut(s) 116, 169, 523, 613, 813, 1196, 1247, 1288
Alw21I GWGCWC 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 758, 760
AlwI GGATC 2 cut(s) 250, 902
Ama87I CYCGRG 1 cut(s) 1058
AoxI GGCC 5 cut(s) 33, 443, 550, 565, 1370
ApoI RAATTY 3 cut(s) 149, 1123, 1222
AsiGI ACCGGT 1 cut(s) 782
Asp700I GAANNNNTTC 2 cut(s) 797, 1183
AspS9I GGNCC 2 cut(s) 34, 444
AsuC2I CCSGG 1 cut(s) 146
AsuHPI GGTGA 4 cut(s) 220, 503, 772, 1262
AsuII TTCGAA 1 cut(s) 1187
AvaI CYCGRG 1 cut(s) 1058
BalI TGGCCA 1 cut(s) 1372
BanI GGYRCC 1 cut(s) 1348
BanII GRGCYC 2 cut(s) 118, 459
BbsI GAAGAC 1 cut(s) 990
Bbv12I GWGCWC 1 cut(s) 118
BbvCI CCTCAGC 1 cut(s) 633
BccI CCATC 5 cut(s) 357, 414, 579, 998, 1245
BciT130I CCWGG 2 cut(s) 887, 1393
BcnI CCSGG 1 cut(s) 146
BcoDI GTCTC 2 cut(s) 758, 760
BfaI CTAG 3 cut(s) 810, 896, 1139
BfmI CTRYAG 3 cut(s) 733, 852, 1357
BglI GCCNNNNNGGC 1 cut(s) 139
BisI GCNGC 4 cut(s) 140, 440, 443, 568
BlsI GCNGC 4 cut(s) 141, 441, 444, 569
Bme1390I CCNGG 3 cut(s) 146, 887, 1393
BmeT110I CYCGRG 1 cut(s) 1058
BmgBI CACGTC 1 cut(s) 914
BmgT120I GGNCC 2 cut(s) 34, 444
BmiI GGNNCC 3 cut(s) 143, 596, 1350
BmrFI CCNGG 3 cut(s) 146, 887, 1393
BmrI ACTGGG 1 cut(s) 593
BmsI GCATC 2 cut(s) 202, 685
BmuI ACTGGG 1 cut(s) 593
BpiI GAAGAC 1 cut(s) 990
BplI GAGNNNNNCTC 4 cut(s) 755, 787, 949, 981
Bpu10I CCTNAGC 1 cut(s) 633
Bpu14I TTCGAA 1 cut(s) 1187
BpuEI CTTGAG 2 cut(s) 149, 951
BpuMI CCSGG 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 1085
BsaJI CCNNGG 6 cut(s) 415, 836, 885, 886, 1174, 1391
BsaWI WCCGGW 1 cut(s) 782
BsaXI ACNNNNNCTCC 2 cut(s) 59, 89
Bsc4I CCNNNNNNNGG 4 cut(s) 709, 789, 917, 1175
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 3 cut(s) 50, 599, 878
Bse3DI GCAATG 2 cut(s) 827, 1105
BseBI CCWGG 2 cut(s) 887, 1393
BseCI ATCGAT 1 cut(s) 1085
BseDI CCNNGG 6 cut(s) 415, 836, 885, 886, 1174, 1391
BseGI GGATG 5 cut(s) 243, 571, 1256, 1339, 1401
BseLI CCNNNNNNNGG 4 cut(s) 709, 789, 917, 1175
BseMI GCAATG 2 cut(s) 827, 1105
BseMII CTCAG 2 cut(s) 186, 647
BseNI ACTGG 3 cut(s) 50, 599, 878
BseRI GAGGAG 4 cut(s) 108, 111, 114, 117
BsgI GTGCAG 1 cut(s) 495
Bsh1236I CGCG 1 cut(s) 139
BshFI GGCC 5 cut(s) 35, 445, 552, 567, 1372
BshNI GGYRCC 1 cut(s) 1348
BshTI ACCGGT 1 cut(s) 782
BshVI ATCGAT 1 cut(s) 1085
BsiHKAI GWGCWC 1 cut(s) 118
BsiHKCI CYCGRG 1 cut(s) 1058
BsiSI CCGG 3 cut(s) 111, 146, 783
BslFI GGGAC 1 cut(s) 1374
BslI CCNNNNNNNGG 4 cut(s) 709, 789, 917, 1175
BsmAI GTCTC 2 cut(s) 758, 760
BsmFI GGGAC 1 cut(s) 1374
BsmI GAATGC 3 cut(s) 662, 1183, 1208
BsnI GGCC 5 cut(s) 35, 445, 552, 567, 1372
BsoBI CYCGRG 1 cut(s) 1058
Bsp119I TTCGAA 1 cut(s) 1187
Bsp1286I GDGCHC 2 cut(s) 118, 459
Bsp143I GATC 4 cut(s) 56, 255, 907, 1086
Bsp19I CCATGG 1 cut(s) 1174
BspACI CCGC 7 cut(s) 131, 139, 439, 442, 459, 568, 1065
BspANI GGCC 5 cut(s) 35, 445, 552, 567, 1372
BspCNI CTCAG 2 cut(s) 185, 646
BspDI ATCGAT 1 cut(s) 1085
BspFNI CGCG 1 cut(s) 139
BspHI TCATGA 1 cut(s) 217
BspLI GGNNCC 3 cut(s) 143, 596, 1350
BspMAI CTGCAG 1 cut(s) 737
BspPI GGATC 2 cut(s) 250, 902
BspQI GCTCTTC 2 cut(s) 820, 1384
BspT104I TTCGAA 1 cut(s) 1187
BspT107I GGYRCC 1 cut(s) 1348
BsrDI GCAATG 2 cut(s) 827, 1105
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 3 cut(s) 50, 599, 878
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 6 cut(s) 415, 836, 885, 886, 1174, 1391
BssMI GATC 4 cut(s) 56, 255, 907, 1086
BssT1I CCWWGG 2 cut(s) 836, 1174
Bst2UI CCWGG 2 cut(s) 887, 1393
Bst4CI ACNGT 8 cut(s) 88, 589, 673, 686, 754, 853, 1345, 1365
Bst6I CTCTTC 5 cut(s) 302, 672, 778, 820, 1384
BstAPI GCANNNNNTGC 1 cut(s) 212
BstBI TTCGAA 1 cut(s) 1187
BstC8I GCNNGC 2 cut(s) 437, 459
BstDEI CTNAG 2 cut(s) 172, 633
BstDSI CCRYGG 1 cut(s) 1174
BstF5I GGATG 5 cut(s) 243, 571, 1256, 1339, 1401
BstFNI CGCG 1 cut(s) 139
BstKTI GATC 4 cut(s) 59, 258, 910, 1089
BstMAI GTCTC 2 cut(s) 758, 760
BstMBI GATC 4 cut(s) 56, 255, 907, 1086
BstMWI GCNNNNNNNGC 2 cut(s) 139, 212
BstNI CCWGG 2 cut(s) 887, 1393
BstNSI RCATGY 1 cut(s) 439
BstSCI CCNGG 3 cut(s) 144, 885, 1391
BstSFI CTRYAG 3 cut(s) 733, 852, 1357
BstUI CGCG 1 cut(s) 139
BstV2I GAAGAC 1 cut(s) 990
BstX2I RGATCY 1 cut(s) 255
BstYI RGATCY 1 cut(s) 255
Bsu15I ATCGAT 1 cut(s) 1085
BsuRI GGCC 5 cut(s) 35, 445, 552, 567, 1372
BsuTUI ATCGAT 1 cut(s) 1085
BtgI CCRYGG 1 cut(s) 1174
BtgZI GCGATG 1 cut(s) 1166
BtrI CACGTC 1 cut(s) 914
BtsCI GGATG 5 cut(s) 243, 571, 1256, 1339, 1401
BtsIMutI CAGTG 4 cut(s) 57, 669, 871, 1350
Cac8I GCNNGC 2 cut(s) 437, 459
CciI TCATGA 1 cut(s) 217
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 34, 444
ClaI ATCGAT 1 cut(s) 1085
CseI GACGC 2 cut(s) 169, 821
Csp6I GTAC 3 cut(s) 786, 1032, 1132
CspAI ACCGGT 1 cut(s) 782
CviAII CATG 7 cut(s) 218, 251, 356, 436, 658, 789, 1175
CviQI GTAC 3 cut(s) 786, 1032, 1132
DdeI CTNAG 2 cut(s) 172, 633
DpnI GATC 4 cut(s) 58, 257, 909, 1088
DpnII GATC 4 cut(s) 56, 255, 907, 1086
EaeI YGGCCR 2 cut(s) 565, 1370
Eam1104I CTCTTC 5 cut(s) 302, 672, 778, 820, 1384
EarI CTCTTC 5 cut(s) 302, 672, 778, 820, 1384
EciI GGCGGA 2 cut(s) 120, 1080
Ecl136II GAGCTC 1 cut(s) 116
Eco130I CCWWGG 2 cut(s) 836, 1174
Eco24I GRGCYC 2 cut(s) 118, 459
Eco32I GATATC 1 cut(s) 1304
Eco53kI GAGCTC 1 cut(s) 116
Eco57I CTGAAG 1 cut(s) 984
Eco88I CYCGRG 1 cut(s) 1058
EcoICRI GAGCTC 1 cut(s) 116
EcoRI GAATTC 2 cut(s) 149, 1222
EcoRII CCWGG 2 cut(s) 885, 1391
EcoRV GATATC 1 cut(s) 1304
EcoT14I CCWWGG 2 cut(s) 836, 1174
EcoT38I GRGCYC 2 cut(s) 118, 459
ErhI CCWWGG 2 cut(s) 836, 1174
FaeI CATG 7 cut(s) 221, 254, 359, 439, 661, 792, 1178
FalI AAGNNNNNCTT 2 cut(s) 1185, 1217
FaqI GGGAC 1 cut(s) 1374
FatI CATG 7 cut(s) 217, 250, 355, 435, 657, 788, 1174
FauI CCCGC 1 cut(s) 466
Fnu4HI GCNGC 4 cut(s) 140, 440, 443, 568
FokI GGATG 4 cut(s) 230, 558, 1263, 1346
FriOI GRGCYC 2 cut(s) 118, 459
Fsp4HI GCNGC 4 cut(s) 140, 440, 443, 568
FspBI CTAG 3 cut(s) 810, 896, 1139
GluI GCNGC 4 cut(s) 140, 440, 443, 568
HaeIII GGCC 5 cut(s) 35, 445, 552, 567, 1372
HapII CCGG 3 cut(s) 111, 146, 783
HgaI GACGC 2 cut(s) 169, 821
Hin1II CATG 7 cut(s) 221, 254, 359, 439, 661, 792, 1178
HindIII AAGCTT 1 cut(s) 1286
HinfI GANTC 6 cut(s) 72, 93, 743, 1165, 1297, 1324
HpaII CCGG 3 cut(s) 111, 146, 783
HphI GGTGA 4 cut(s) 220, 503, 772, 1262
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 7 cut(s) 61, 175, 419, 1003, 1026, 1221, 1228
Hpy188III TCNNGA 6 cut(s) 157, 218, 968, 1058, 1169, 1313
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 7 cut(s) 56, 345, 482, 563, 947, 1223, 1329
HpyCH4III ACNGT 8 cut(s) 88, 589, 673, 686, 754, 853, 1345, 1365
HpyCH4IV ACGT 1 cut(s) 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 212
HpyF3I CTNAG 2 cut(s) 172, 633
HpySE526I ACGT 1 cut(s) 913
Hsp92II CATG 7 cut(s) 221, 254, 359, 439, 661, 792, 1178
Kzo9I GATC 4 cut(s) 56, 255, 907, 1086
LguI GCTCTTC 2 cut(s) 820, 1384
LmnI GCTCC 3 cut(s) 113, 121, 147
LweI GCATC 2 cut(s) 202, 685
MaeI CTAG 3 cut(s) 810, 896, 1139
MaeII ACGT 1 cut(s) 913
MaeIII GTNAC 3 cut(s) 527, 728, 1313
MalI GATC 4 cut(s) 58, 257, 909, 1088
MboI GATC 4 cut(s) 56, 255, 907, 1086
MboII GAAGA 9 cut(s) 319, 548, 689, 765, 807, 990, 1126, 1228, 1371
MflI RGATCY 1 cut(s) 255
MhlI GDGCHC 2 cut(s) 118, 459
MlsI TGGCCA 1 cut(s) 1372
MluCI AATT 6 cut(s) 149, 373, 462, 944, 1123, 1222
MluNI TGGCCA 1 cut(s) 1372
MlyI GAGTC 1 cut(s) 102
Mox20I TGGCCA 1 cut(s) 1372
MroXI GAANNNNTTC 2 cut(s) 797, 1183
MscI TGGCCA 1 cut(s) 1372
MseI TTAA 7 cut(s) 23, 293, 449, 690, 858, 942, 1290
MslI CAYNNNNRTG 1 cut(s) 1346
Msp20I TGGCCA 1 cut(s) 1372
MspI CCGG 3 cut(s) 111, 146, 783
MspR9I CCNGG 3 cut(s) 146, 887, 1393
Mva1269I GAATGC 3 cut(s) 662, 1183, 1208
MvaI CCWGG 2 cut(s) 887, 1393
MvnI CGCG 1 cut(s) 139
MwoI GCNNNNNNNGC 2 cut(s) 139, 212
NciI CCSGG 1 cut(s) 146
NcoI CCATGG 1 cut(s) 1174
NdeII GATC 4 cut(s) 56, 255, 907, 1086
NlaIII CATG 7 cut(s) 221, 254, 359, 439, 661, 792, 1178
NlaIV GGNNCC 3 cut(s) 143, 596, 1350
NmuCI GTSAC 1 cut(s) 1313
NspI RCATGY 1 cut(s) 439
NspV TTCGAA 1 cut(s) 1187
OliI CACNNNNGTG 1 cut(s) 1346
PaeI GCATGC 1 cut(s) 439
PaeR7I CTCGAG 1 cut(s) 1058
PagI TCATGA 1 cut(s) 217
PasI CCCWGGG 1 cut(s) 886
PciSI GCTCTTC 2 cut(s) 820, 1384
PctI GAATGC 3 cut(s) 662, 1183, 1208
PdmI GAANNNNTTC 2 cut(s) 797, 1183
PfeI GAWTC 5 cut(s) 72, 743, 1165, 1297, 1324
PflMI CCANNNNNTGG 2 cut(s) 917, 1175
PfoI TCCNGGA 1 cut(s) 144
PinAI ACCGGT 1 cut(s) 782
PkrI GCNGC 4 cut(s) 141, 441, 444, 569
PleI GAGTC 1 cut(s) 101
PpsI GAGTC 1 cut(s) 101
Psp124BI GAGCTC 1 cut(s) 118
Psp6I CCWGG 2 cut(s) 885, 1391
PspGI CCWGG 2 cut(s) 885, 1391
PspN4I GGNNCC 3 cut(s) 143, 596, 1350
PspPI GGNCC 2 cut(s) 34, 444
PstI CTGCAG 1 cut(s) 737
PsuI RGATCY 1 cut(s) 255
RsaI GTAC 3 cut(s) 787, 1033, 1133
RsaNI GTAC 3 cut(s) 786, 1032, 1132
RseI CAYNNNNRTG 1 cut(s) 1346
SacI GAGCTC 1 cut(s) 118
SapI GCTCTTC 2 cut(s) 820, 1384
SaqAI TTAA 7 cut(s) 23, 293, 449, 690, 858, 942, 1290
SatI GCNGC 4 cut(s) 140, 440, 443, 568
Sau3AI GATC 4 cut(s) 56, 255, 907, 1086
Sau96I GGNCC 2 cut(s) 34, 444
SchI GAGTC 1 cut(s) 102
ScrFI CCNGG 3 cut(s) 146, 887, 1393
SduI GDGCHC 2 cut(s) 118, 459
SfaNI GCATC 2 cut(s) 202, 685
SfcI CTRYAG 3 cut(s) 733, 852, 1357
Sfr274I CTCGAG 1 cut(s) 1058
SfuI TTCGAA 1 cut(s) 1187
SlaI CTCGAG 1 cut(s) 1058
SmiMI CAYNNNNRTG 1 cut(s) 1346
SmlI CTYRAG 3 cut(s) 164, 966, 1058
SmoI CTYRAG 3 cut(s) 164, 966, 1058
SphI GCATGC 1 cut(s) 439
Sse9I AATT 6 cut(s) 149, 373, 462, 944, 1123, 1222
SsiI CCGC 7 cut(s) 131, 139, 439, 442, 459, 568, 1065
SspMI CTAG 3 cut(s) 810, 896, 1139
SstI GAGCTC 1 cut(s) 118
StyD4I CCNGG 3 cut(s) 144, 885, 1391
StyI CCWWGG 2 cut(s) 836, 1174
TaaI ACNGT 8 cut(s) 88, 589, 673, 686, 754, 853, 1345, 1365
TaiI ACGT 1 cut(s) 916
TaqI TCGA 9 cut(s) 305, 343, 361, 675, 1059, 1085, 1187, 1300, 1322
TasI AATT 6 cut(s) 149, 373, 462, 944, 1123, 1222
TatI WGTACW 2 cut(s) 1031, 1131
TauI GCSGC 4 cut(s) 142, 442, 445, 570
TfiI GAWTC 5 cut(s) 72, 743, 1165, 1297, 1324
Tru1I TTAA 7 cut(s) 23, 293, 449, 690, 858, 942, 1290
Tru9I TTAA 7 cut(s) 23, 293, 449, 690, 858, 942, 1290
TscAI CASTG 4 cut(s) 57, 676, 878, 1350
TseFI GTSAC 1 cut(s) 1313
Tsp45I GTSAC 1 cut(s) 1313
TspDTI ATGAA 6 cut(s) 17, 142, 234, 455, 1205, 1228
TspGWI ACGGA 1 cut(s) 254
TspRI CASTG 4 cut(s) 57, 676, 878, 1350
Van91I CCANNNNNTGG 2 cut(s) 917, 1175
XapI RAATTY 3 cut(s) 149, 1123, 1222
XceI RCATGY 1 cut(s) 439
XhoI CTCGAG 1 cut(s) 1058
XmnI GAANNNNTTC 2 cut(s) 797, 1183
XspI CTAG 3 cut(s) 810, 896, 1139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.