pycom13g00280
ERF Family

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Myosin family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005277
Physical Location & Seq
Reverse (-)
194588 .. 202280
7693 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2955 bp
ATGCAACTTAGTTACCTAAATGAGCCATCAGTGTTGTACAATCTTCAGTATAGATACAATCAGGATATGATTTACACAAAAGCGGGACCGGTGTTGGTTGCTGTCAATCCCTTTAAGAGAGTTTCTTTATATGGGAATGAATACATTGAAGCATACAAGCGTAAAGCAGTTGAAAGTCCACATGTGTATGCCATTGCAGACACAGCCATTCGGGAAATGGTTAGAGATGAAGTAAATCAGTCTATAATTATAAGTGGGGAAAGTGGAGCAGGAAAAACTGAAACTGCGAAGATAGCAATGCAATATTTGGCTGCACTTGGAGGTGGAAGTGGAATAGAGCATGAAATTTTGAAAACTAACCCGATACTGGAAGCCTTTGGTAATGCGAAAACATTGAGAAATGACAACTCAAGTCGCTTTGGAAAATTGATTGAAATCCACTTTAGTGAAACTGGAAAAATATCTGGTGCCAATATTCAAACTTTTTTGCTTGAAAAGTCTAGAGTGGTCCAATGCACTGAAGGTGAAAGGTCCTATCATATATTTTATCAGCTTTGTGCTGGAGCTCCACCTGCTCTTAGAGAGATGCTGAACTTGAAGAGTGCAGATGAGTACCAATATCTGAAGCAAAGCAATTGTTATTCCGTTTCTGGGGTGAATGATGCTGAAGAATTCAGTGTGGTGAAGGAAGCTCTAGATGTTGTCCATATAAACAAAGAAGACCAGCAAAGTGTATTTGCAATGCTTGCTGCAGTTTTGTGGCTGGGAAACATCTCATTTAGTGTGATTGATAATGAAAATCATGTTGAACCTGTAGAAGATGAAGGTTTGTTCAATGTCGCAAAATTGATTGGATGTGGCGTAGATGAGCTTAAGCTTGCTTTATCAACTCGAAATATGAGAGTTGGTAATGACAGTATTGTCCAAAAGCTAACGCTATCACAGGCCACCGACACAAGAGATGCTTTGGCAAAGTCAATTTATGCATGTCTGTTTGAGTGGCTGGTTGAACAAATTAACAAATCACTTGCAGTAGGAAAACGGCGTACTGGCAGATCTATCAGCATTCTTGATATCTATGGTTTTGAATCATTTGATAGAAATAGTTTTGAACAGTTCTGCATTAATTATGCAAATGAGAGATTGCAACAACACTTCAATCGTCACTTATTCAAGTTAGAGCAGGATGAATATATCCAAGACGGAATTGATTGGACAAAAGTTGAATTTGAAGACAACCAAGATTGTCTTGGTCTTTTTGAAAAGAGGCCGTTGGGATTACTGTCCCTGTTAGATGAGGAGTCCACCTTCCCAAATGGTACTGATTTGACCTTTGCCAACAAGCTTAAGCAACATCTGAGTTCTAATTCTTGTTTCAAAGGAGAACGAGACAAATCGTTTGCTGTCAGTCATTATGCAGGAGAGGTTTCATATGACACAACAGGATTTTTGGAGAAGAACAGGGACTTACTGCATTTAGATTCTATTCAGCTTCTTTCATCATGCTCATGCCACCTTCCTCAGATATTCGCATCTAGTATGCTTAATCGACCCGAGAAGCCTTTAGTTGGTCCCCTGTATAAAGCAGGTGGCGGAGTAGACTCCCAGAAGCTGAGTGTTGCAACGAAATTTAAGAGTCAATTGTTCCTATTGATGAAACGTCTAGAAAACACCACTCCACATTTCATACGTTGTATCAAGCCCAACAATCTTCAATCCCCTGGACTATATGAGCAAGGACTTGTACTGCAGCAGCTAAGATGTTGTGGAGTTCTAGAAGTGGTTCGAATATCAAGATCTGGCTTTCCTACCAGAATGTCACACCAGAAGTTTGCAAGAAGATATGGTTTTCTTCTGCTGGAGAATGTTGCATCGCAGGATCCACTTAGTGTTTCAGTAGCTATTCTTCACCAGTTCAACATATTAACAGAGATGTATCAAGTGGGCTATACAAAGTTGTTTTTCCGAACTGGACAGATTGGGGTGCTTGAGGACACCAGAAACCGTACGCTACATGGTATTTTGCGTGTTCAAAGCTGCTTTAGAGGACACCAAGCTCGGTGTTACCTCAAGGAGCTGAGGAGAGGAATCACCATGCTCCAATCATTCGTAAGGGGACAGAAAACCAGGAAGGAGTACTCAATATTACTAGAGAGGCATAGAGCTGCTGTTGTCATACAAAAGCAGGTCAAAAGCAGTTCTTCGTGGCTGGTTGGTCAGAAAATGCTCAGGAAGTATAGGATTGTTGAAGCCTGGAAGCATGAAGGTAGTCCTTTAAAGAATCTCAAACAGGCTAACGAGTCAGATGAGGTGCTGGTAAAGGCATCTTTCCTTGCTGAACTACAGCGCCGCGTTCTTAAGGCTGAAGCTGCCTTGCGAGAGAAGGAAGAGGAGAATGACATCCTCCACCAACGTCTCCAACAGTATGAGAGCCGCTGGTCGGAATATGAGCTGAAAATGAAATCCATGGAAGAAGTGTGGCAGAAACAAATGAGATCCCTACAATCCAGCCTCTCAATTGCGAAGAAGAGCTTAGCCATTGATGATTCTGGGAGAAATTCTGATGCATCAGTGAATGCAAGTGATGACCGTGACCAGAGTTGGGATACAGGAAGTAACCATAGAGGCCAAGATAGCAATGGGGTGAGACCATTGAGTGCAGGTTTGAGTGTCATAAGCCGGTTAACTGAAGAGTTCGATCAGAGGAGTCAAGTTTTTGGCGACGATGCCAAGTTCTTGGTGGAGGTGAAGTCTGGTAAAGTGGAAGCAAGTCTGAATCCAGACCAAGAGCTAAGAAGGTTGAAGCAGATGTTTGAGGCTTGGAAGAAGGATTACGGGGCAAGACTAAGGGAGACAAAGTTGATTTTGCATAAGATTGGGAATGAAGAGGGAGGCAGTGCTGACAGGGTGAAAAAGAAGTGGTGGGGAAGGCGGAACAGCTCGAGGATAAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000146 GO:0000166 GO:0000278 GO:0000281 GO:0000302 GO:0000910 GO:0001891 GO:0001931 GO:0003674 GO:0003774 GO:0003779 GO:0003824 GO:0005488 GO:0005515 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005826 GO:0005829 GO:0005856 GO:0005886 GO:0005938 GO:0006928 GO:0006935 GO:0006950 GO:0006970 GO:0006971 GO:0006979 GO:0006996 GO:0007010 GO:0007015 GO:0007033 GO:0007049 GO:0007154 GO:0008064 GO:0008092 GO:0008104 GO:0008144 GO:0008150 GO:0008360 GO:0009267 GO:0009581 GO:0009582 GO:0009605 GO:0009612 GO:0009628 GO:0009636 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010243 GO:0010639 GO:0014070 GO:0014074 GO:0015629 GO:0016020 GO:0016043 GO:0016050 GO:0016459 GO:0016460 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0022402 GO:0022603 GO:0022604 GO:0022607 GO:0030029 GO:0030030 GO:0030031 GO:0030036 GO:0030038 GO:0030048 GO:0030139 GO:0030554 GO:0030587 GO:0030832 GO:0030833 GO:0030837 GO:0030863 GO:0030864 GO:0030865 GO:0030866 GO:0030898 GO:0031033 GO:0031034 GO:0031152 GO:0031154 GO:0031254 GO:0031268 GO:0031270 GO:0031333 GO:0031410 GO:0031667 GO:0031668 GO:0031669 GO:0031982 GO:0032009 GO:0032060 GO:0032153 GO:0032155 GO:0032271 GO:0032272 GO:0032502 GO:0032535 GO:0032553 GO:0032555 GO:0032559 GO:0032796 GO:0032956 GO:0032970 GO:0032982 GO:0032991 GO:0033036 GO:0033043 GO:0033275 GO:0033298 GO:0033554 GO:0034461 GO:0034622 GO:0035639 GO:0036094 GO:0040011 GO:0042221 GO:0042330 GO:0042493 GO:0042542 GO:0042594 GO:0042623 GO:0042641 GO:0042802 GO:0042803 GO:0042995 GO:0043167 GO:0043168 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043232 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044422 GO:0044424 GO:0044425 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044459 GO:0044464 GO:0044764 GO:0044877 GO:0045177 GO:0045179 GO:0045335 GO:0046677 GO:0046683 GO:0046847 GO:0046983 GO:0048519 GO:0048523 GO:0048856 GO:0048870 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0050982 GO:0051015 GO:0051017 GO:0051128 GO:0051129 GO:0051179 GO:0051301 GO:0051493 GO:0051494 GO:0051591 GO:0051606 GO:0051674 GO:0051703 GO:0051704 GO:0051716 GO:0060327 GO:0060328 GO:0061572 GO:0061640 GO:0065003 GO:0065007 GO:0065008 GO:0070252 GO:0070938 GO:0071496 GO:0071840 GO:0071889 GO:0071944 GO:0090066 GO:0090702 GO:0097159 GO:0097204 GO:0097367 GO:0097435 GO:0097708 GO:0098630 GO:0098743 GO:0099120 GO:0099568 GO:0099738 GO:0110053 GO:0120025 GO:0120031 GO:0120036 GO:1901265 GO:1901363 GO:1901698 GO:1901700 GO:1902903 GO:1902904 GO:1903047 GO:1990753
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
IQ Myosin_head

Protein Analysis

985

Amino Acids

111.89

Weight (kDa)

8.66

Isoelectric Point (pI)

48.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Myosin_head PF00063 2 - 656 1.2e-242 Myosin head (motor domain)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 251
AarI CACCTGC 2 cut(s) 580, 1577
AasI GACNNNNNNGTC 1 cut(s) 920
Acc36I ACCTGC 4 cut(s) 580, 1577, 2176, 2651
AccB1I GGYRCC 1 cut(s) 467
AccI GTMKAC 1 cut(s) 1599
AccII CGCG 1 cut(s) 2352
AciI CCGC 5 cut(s) 83, 1593, 2350, 2434, 2932
AclWI GGATC 3 cut(s) 1874, 1887, 2490
AcsI RAATTY 5 cut(s) 345, 671, 1226, 1628, 2557
AcuI CTGAAG 6 cut(s) 29, 540, 644, 687, 2385, 2709
AdeI CACNNNGTG 1 cut(s) 1889
AfaI GTAC 7 cut(s) 38, 614, 1048, 1321, 1746, 2008, 2138
AfiI CCNNNNNNNGG 5 cut(s) 367, 651, 1568, 2440, 2602
AflII CTTAAG 3 cut(s) 872, 1346, 2357
AflIII ACRYGT 1 cut(s) 181
AgeI ACCGGT 1 cut(s) 88
AjnI CCWGG 3 cut(s) 1720, 2126, 2252
AleI CACNNNNGTG 1 cut(s) 444
Alw21I GWGCWC 1 cut(s) 568
Alw26I GTCTC 4 cut(s) 1383, 2420, 2641, 2846
AlwI GGATC 3 cut(s) 1874, 1887, 2490
AlwNI CAGNNNCTG 1 cut(s) 1612
Ama87I CYCGRG 2 cut(s) 1553, 2941
AoxI GGCC 3 cut(s) 945, 1268, 2626
ApeKI GCWGC 7 cut(s) 311, 749, 1750, 1753, 2037, 2165, 2369
ApoI RAATTY 5 cut(s) 345, 671, 1226, 1628, 2557
ArsI GACNNNNNNTTYG 2 cut(s) 1382, 1414
AseI ATTAAT 1 cut(s) 1125
AsiGI ACCGGT 1 cut(s) 88
Asp700I GAANNNNTTC 1 cut(s) 1782
AspLEI GCGC 1 cut(s) 2349
AspS9I GGNCC 4 cut(s) 86, 508, 531, 1571
AsuHPI GGTGA 8 cut(s) 536, 667, 694, 1901, 2083, 2656, 2758, 2920
AsuII TTCGAA 1 cut(s) 1786
AvaI CYCGRG 2 cut(s) 1553, 2941
AvaII GGWCC 4 cut(s) 86, 508, 531, 1571
BamHI GGATCC 1 cut(s) 1879
BanI GGYRCC 1 cut(s) 467
BanII GRGCYC 1 cut(s) 568
BarI GAAGNNNNNNTAC 4 cut(s) 1890, 1922, 2816, 2848
BbsI GAAGAC 2 cut(s) 726, 1239
Bbv12I GWGCWC 1 cut(s) 568
BbvCI CCTCAGC 1 cut(s) 2078
BbvI GCAGC 7 cut(s) 298, 736, 1762, 1765, 2024, 2152, 2356
BccI CCATC 1 cut(s) 34
BceAI ACGGC 2 cut(s) 1058, 1255
BciT130I CCWGG 3 cut(s) 1722, 2128, 2254
BciVI GTATCC 1 cut(s) 2599
BcoDI GTCTC 4 cut(s) 1383, 2420, 2641, 2846
BfaI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
BfmI CTRYAG 4 cut(s) 750, 813, 1748, 2342
BfoI RGCGCY 1 cut(s) 2350
BfrI CTTAAG 3 cut(s) 872, 1346, 2357
BfuAI ACCTGC 4 cut(s) 580, 1577, 2176, 2651
BfuI GTATCC 1 cut(s) 2599
BglII AGATCT 2 cut(s) 1055, 1796
BisI GCNGC 9 cut(s) 312, 750, 1751, 1754, 2038, 2166, 2350, 2370, 2434
BlpI GCTNAGC 1 cut(s) 2533
BlsI GCNGC 9 cut(s) 313, 751, 1752, 1755, 2039, 2167, 2351, 2371, 2435
BmcAI AGTACT 1 cut(s) 2138
Bme1390I CCNGG 3 cut(s) 1722, 2128, 2254
Bme18I GGWCC 4 cut(s) 86, 508, 531, 1571
BmeT110I CYCGRG 2 cut(s) 1553, 2941
BmgT120I GGNCC 4 cut(s) 86, 508, 531, 1571
BmiI GGNNCC 4 cut(s) 87, 469, 1573, 1881
BmrFI CCNGG 3 cut(s) 1722, 2128, 2254
BmsI GCATC 9 cut(s) 576, 652, 952, 1541, 1880, 2333, 2554, 2576, 2716
BpiI GAAGAC 2 cut(s) 726, 1239
BpmI CTGGAG 2 cut(s) 582, 1880
Bpu10I CCTNAGC 2 cut(s) 2078, 2228
Bpu1102I GCTNAGC 1 cut(s) 2533
Bpu14I TTCGAA 1 cut(s) 1786
BpuEI CTTGAG 3 cut(s) 394, 2009, 2054
BsaBI GATNNNNATC 1 cut(s) 434
BsaI GGTCTC 1 cut(s) 2641
BsaJI CCNNGG 2 cut(s) 1720, 2466
BsaWI WCCGGW 1 cut(s) 88
BsaXI ACNNNNNCTCC 4 cut(s) 2735, 2765, 2843, 2873
Bsc4I CCNNNNNNNGG 5 cut(s) 367, 651, 1568, 2440, 2602
Bse118I RCCGGY 2 cut(s) 88, 2679
Bse1I ACTGG 5 cut(s) 372, 457, 1054, 1912, 1975
Bse3DI GCAATG 4 cut(s) 192, 303, 747, 2644
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 3 cut(s) 1722, 2128, 2254
BseDI CCNNGG 2 cut(s) 1720, 2466
BseGI GGATG 3 cut(s) 860, 1192, 2400
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 5 cut(s) 367, 651, 1568, 2440, 2602
BseMI GCAATG 4 cut(s) 192, 303, 747, 2644
BseMII CTCAG 5 cut(s) 1349, 1535, 1604, 2069, 2242
BseNI ACTGG 5 cut(s) 372, 457, 1054, 1912, 1975
BseRI GAGGAG 4 cut(s) 1313, 2095, 2405, 2719
BseXI GCAGC 7 cut(s) 298, 736, 1762, 1765, 2024, 2152, 2356
BseYI CCCAGC 1 cut(s) 763
BsgI GTGCAG 3 cut(s) 297, 624, 2679
Bsh1236I CGCG 1 cut(s) 2352
BshFI GGCC 3 cut(s) 947, 1270, 2628
BshNI GGYRCC 1 cut(s) 467
BshTI ACCGGT 1 cut(s) 88
BsiHKAI GWGCWC 1 cut(s) 568
BsiHKCI CYCGRG 2 cut(s) 1553, 2941
BsiSI CCGG 2 cut(s) 89, 2680
BsiWI CGTACG 1 cut(s) 2006
BslFI GGGAC 5 cut(s) 99, 1270, 1478, 1557, 2130
BslI CCNNNNNNNGG 5 cut(s) 367, 651, 1568, 2440, 2602
BsmAI GTCTC 4 cut(s) 1383, 2420, 2641, 2846
BsmBI CGTCTC 1 cut(s) 2420
BsmFI GGGAC 5 cut(s) 99, 1270, 1478, 1557, 2130
BsmI GAATGC 2 cut(s) 1065, 2581
BsnI GGCC 3 cut(s) 947, 1270, 2628
Bso31I GGTCTC 1 cut(s) 2641
BsoBI CYCGRG 2 cut(s) 1553, 2941
Bsp119I TTCGAA 1 cut(s) 1786
Bsp1286I GDGCHC 1 cut(s) 568
Bsp1407I TGTACA 1 cut(s) 36
Bsp143I GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
Bsp1720I GCTNAGC 1 cut(s) 2533
Bsp19I CCATGG 1 cut(s) 2466
BspACI CCGC 5 cut(s) 83, 1593, 2350, 2434, 2932
BspANI GGCC 3 cut(s) 947, 1270, 2628
BspCNI CTCAG 5 cut(s) 1350, 1534, 1605, 2070, 2241
BspFNI CGCG 1 cut(s) 2352
BspLI GGNNCC 4 cut(s) 87, 469, 1573, 1881
BspMAI CTGCAG 2 cut(s) 754, 1752
BspMI ACCTGC 4 cut(s) 580, 1577, 2176, 2651
BspPI GGATC 3 cut(s) 1874, 1887, 2490
BspQI GCTCTTC 1 cut(s) 2522
BspT104I TTCGAA 1 cut(s) 1786
BspT107I GGYRCC 1 cut(s) 467
BspTI CTTAAG 3 cut(s) 872, 1346, 2357
BspTNI GGTCTC 1 cut(s) 2641
BsrDI GCAATG 4 cut(s) 192, 303, 747, 2644
BsrFI RCCGGY 2 cut(s) 88, 2679
BsrGI TGTACA 1 cut(s) 36
BsrI ACTGG 5 cut(s) 372, 457, 1054, 1912, 1975
BssAI RCCGGY 2 cut(s) 88, 2679
BssECI CCNNGG 2 cut(s) 1720, 2466
BssMI GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
BssT1I CCWWGG 1 cut(s) 2466
Bst2UI CCWGG 3 cut(s) 1722, 2128, 2254
Bst4CI ACNGT 6 cut(s) 917, 1116, 1284, 2006, 2424, 2591
Bst6I CTCTTC 5 cut(s) 593, 2382, 2522, 2685, 2880
BstAFI CTTAAG 3 cut(s) 872, 1346, 2357
BstAPI GCANNNNNTGC 1 cut(s) 746
BstAUI TGTACA 1 cut(s) 36
BstBI TTCGAA 1 cut(s) 1786
BstC8I GCNNGC 2 cut(s) 747, 879
BstDSI CCRYGG 1 cut(s) 2466
BstF5I GGATG 3 cut(s) 860, 1192, 2400
BstFNI CGCG 1 cut(s) 2352
BstH2I RGCGCY 1 cut(s) 2350
BstHHI GCGC 1 cut(s) 2349
BstKTI GATC 5 cut(s) 1058, 1799, 1882, 2498, 2701
BstMAI GTCTC 4 cut(s) 1383, 2420, 2641, 2846
BstMBI GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
BstMWI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2369, 2634
BstNI CCWGG 3 cut(s) 1722, 2128, 2254
BstNSI RCATGY 2 cut(s) 185, 990
BstSCI CCNGG 3 cut(s) 1720, 2126, 2252
BstSFI CTRYAG 4 cut(s) 750, 813, 1748, 2342
BstUI CGCG 1 cut(s) 2352
BstV1I GCAGC 7 cut(s) 298, 736, 1762, 1765, 2024, 2152, 2356
BstV2I GAAGAC 2 cut(s) 726, 1239
BstX2I RGATCY 4 cut(s) 1055, 1796, 1879, 2495
BstXI CCANNNNNNTGG 1 cut(s) 2737
BstYI RGATCY 4 cut(s) 1055, 1796, 1879, 2495
BsuI GTATCC 1 cut(s) 2599
BsuRI GGCC 3 cut(s) 947, 1270, 2628
BtgI CCRYGG 1 cut(s) 2466
BtgZI GCGATG 1 cut(s) 1857
BtsCI GGATG 3 cut(s) 860, 1192, 2400
BtsI GCAGTG 1 cut(s) 2902
BtsIMutI CAGTG 5 cut(s) 36, 516, 682, 2577, 2902
BveI ACCTGC 4 cut(s) 580, 1577, 2176, 2651
Cac8I GCNNGC 2 cut(s) 747, 879
CaiI CAGNNNCTG 1 cut(s) 1612
CfoI GCGC 1 cut(s) 2349
Cfr10I RCCGGY 2 cut(s) 88, 2679
Cfr13I GGNCC 4 cut(s) 86, 508, 531, 1571
Csp6I GTAC 7 cut(s) 37, 613, 1047, 1320, 1745, 2007, 2137
CspAI ACCGGT 1 cut(s) 88
CviQI GTAC 7 cut(s) 37, 613, 1047, 1320, 1745, 2007, 2137
DpnI GATC 5 cut(s) 1057, 1798, 1881, 2497, 2700
DpnII GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
DraI TTTAAA 1 cut(s) 2277
DraIII CACNNNGTG 1 cut(s) 1889
DrdI GACNNNNNNGTC 1 cut(s) 920
DseDI GACNNNNNNGTC 1 cut(s) 920
Eam1104I CTCTTC 5 cut(s) 593, 2382, 2522, 2685, 2880
EarI CTCTTC 5 cut(s) 593, 2382, 2522, 2685, 2880
EciI GGCGGA 2 cut(s) 1608, 2947
Ecl136II GAGCTC 1 cut(s) 566
Eco130I CCWWGG 1 cut(s) 2466
Eco24I GRGCYC 1 cut(s) 568
Eco31I GGTCTC 1 cut(s) 2641
Eco32I GATATC 1 cut(s) 1075
Eco47I GGWCC 4 cut(s) 86, 508, 531, 1571
Eco53kI GAGCTC 1 cut(s) 566
Eco57I CTGAAG 6 cut(s) 29, 540, 644, 687, 2385, 2709
Eco88I CYCGRG 2 cut(s) 1553, 2941
EcoICRI GAGCTC 1 cut(s) 566
EcoO109I RGGNCCY 1 cut(s) 531
EcoRI GAATTC 1 cut(s) 671
EcoRII CCWGG 3 cut(s) 1720, 2126, 2252
EcoRV GATATC 1 cut(s) 1075
EcoT14I CCWWGG 1 cut(s) 2466
EcoT22I ATGCAT 2 cut(s) 988, 2569
EcoT38I GRGCYC 1 cut(s) 568
ErhI CCWWGG 1 cut(s) 2466
Esp3I CGTCTC 1 cut(s) 2420
FalI AAGNNNNNCTT 8 cut(s) 949, 981, 1233, 1265, 2185, 2217, 2516, 2548
FaqI GGGAC 5 cut(s) 99, 1270, 1478, 1557, 2130
FauI CCCGC 1 cut(s) 76
FauNDI CATATG 1 cut(s) 1432
FblI GTMKAC 1 cut(s) 1599
Fnu4HI GCNGC 9 cut(s) 312, 750, 1751, 1754, 2038, 2166, 2350, 2370, 2434
FokI GGATG 3 cut(s) 867, 1199, 2387
FriOI GRGCYC 1 cut(s) 568
Fsp4HI GCNGC 9 cut(s) 312, 750, 1751, 1754, 2038, 2166, 2350, 2370, 2434
FspBI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
GlaI GCGC 1 cut(s) 2348
GluI GCNGC 9 cut(s) 312, 750, 1751, 1754, 2038, 2166, 2350, 2370, 2434
GsaI CCCAGC 1 cut(s) 767
GsuI CTGGAG 2 cut(s) 582, 1880
HaeII RGCGCY 1 cut(s) 2350
HaeIII GGCC 3 cut(s) 947, 1270, 2628
HapII CCGG 2 cut(s) 89, 2680
HhaI GCGC 1 cut(s) 2349
Hin6I GCGC 1 cut(s) 2347
HinP1I GCGC 1 cut(s) 2347
HincII GTYRAC 1 cut(s) 2685
HindII GTYRAC 1 cut(s) 2685
HindIII AAGCTT 2 cut(s) 875, 1343
HpaI GTTAAC 1 cut(s) 2685
HpaII CCGG 2 cut(s) 89, 2680
HphI GGTGA 8 cut(s) 536, 667, 694, 1901, 2083, 2656, 2758, 2920
Hpy166II GTNNAC 4 cut(s) 179, 1305, 1600, 2685
Hpy8I GTNNAC 4 cut(s) 179, 1305, 1600, 2685
Hpy99I CGWCG 1 cut(s) 2726
HpyCH4III ACNGT 6 cut(s) 917, 1116, 1284, 2006, 2424, 2591
HpyCH4IV ACGT 3 cut(s) 1660, 1690, 2413
HpyF10VI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2369, 2634
HpySE526I ACGT 3 cut(s) 1660, 1690, 2413
HspAI GCGC 1 cut(s) 2347
KspAI GTTAAC 1 cut(s) 2685
Kzo9I GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
LguI GCTCTTC 1 cut(s) 2522
LmnI GCTCC 5 cut(s) 266, 563, 571, 2074, 2103
Lsp1109I GCAGC 7 cut(s) 298, 736, 1762, 1765, 2024, 2152, 2356
LweI GCATC 9 cut(s) 576, 652, 952, 1541, 1880, 2333, 2554, 2576, 2716
MaeI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
MaeII ACGT 3 cut(s) 1660, 1690, 2413
MaeIII GTNAC 6 cut(s) 11, 1163, 1818, 2063, 2591, 2615
MalI GATC 5 cut(s) 1057, 1798, 1881, 2497, 2700
MboI GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
MfeI CAATTG 3 cut(s) 634, 1640, 2517
MflI RGATCY 4 cut(s) 1055, 1796, 1879, 2495
MhlI GDGCHC 1 cut(s) 568
MlyI GAGTC 5 cut(s) 1310, 1595, 1645, 2309, 2716
MmeI TCCRAC 2 cut(s) 2421, 2443
Mph1103I ATGCAT 2 cut(s) 988, 2569
MroXI GAANNNNTTC 1 cut(s) 1782
MslI CAYNNNNRTG 3 cut(s) 186, 444, 1507
MspA1I CMGCKG 1 cut(s) 2436
MspCI CTTAAG 3 cut(s) 872, 1346, 2357
MspI CCGG 2 cut(s) 89, 2680
MspR9I CCNGG 3 cut(s) 1722, 2128, 2254
MunI CAATTG 3 cut(s) 634, 1640, 2517
Mva1269I GAATGC 2 cut(s) 1065, 2581
MvaI CCWGG 3 cut(s) 1722, 2128, 2254
MvnI CGCG 1 cut(s) 2352
MwoI GCNNNNNNNGC 6 cut(s) 203, 293, 572, 746, 2369, 2634
NcoI CCATGG 1 cut(s) 2466
NdeI CATATG 1 cut(s) 1432
NdeII GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
NlaIV GGNNCC 4 cut(s) 87, 469, 1573, 1881
NmuCI GTSAC 3 cut(s) 1163, 1818, 2591
NsiI ATGCAT 2 cut(s) 988, 2569
NspI RCATGY 2 cut(s) 185, 990
NspV TTCGAA 1 cut(s) 1786
OliI CACNNNNGTG 1 cut(s) 444
PaeR7I CTCGAG 1 cut(s) 2941
PaqCI CACCTGC 2 cut(s) 580, 1577
PciI ACATGT 1 cut(s) 181
PciSI GCTCTTC 1 cut(s) 2522
PctI GAATGC 2 cut(s) 1065, 2581
PdmI GAANNNNTTC 1 cut(s) 1782
PfeI GAWTC 6 cut(s) 1088, 1481, 2088, 2281, 2546, 2776
Pfl23II CGTACG 1 cut(s) 2006
PinAI ACCGGT 1 cut(s) 88
PkrI GCNGC 9 cut(s) 313, 751, 1752, 1755, 2039, 2167, 2351, 2371, 2435
PleI GAGTC 5 cut(s) 1309, 1595, 1644, 2308, 2715
PpsI GAGTC 5 cut(s) 1309, 1595, 1644, 2308, 2715
PpuMI RGGWCCY 1 cut(s) 531
PscI ACATGT 1 cut(s) 181
PshBI ATTAAT 1 cut(s) 1125
PsiI TTATAA 1 cut(s) 251
Psp124BI GAGCTC 1 cut(s) 568
Psp5II RGGWCCY 1 cut(s) 531
Psp6I CCWGG 3 cut(s) 1720, 2126, 2252
PspFI CCCAGC 1 cut(s) 763
PspGI CCWGG 3 cut(s) 1720, 2126, 2252
PspLI CGTACG 1 cut(s) 2006
PspN4I GGNNCC 4 cut(s) 87, 469, 1573, 1881
PspPI GGNCC 4 cut(s) 86, 508, 531, 1571
PspPPI RGGWCCY 1 cut(s) 531
PspXI VCTCGAGB 1 cut(s) 2941
PstI CTGCAG 2 cut(s) 754, 1752
PstNI CAGNNNCTG 1 cut(s) 1612
PsuI RGATCY 4 cut(s) 1055, 1796, 1879, 2495
RsaI GTAC 7 cut(s) 38, 614, 1048, 1321, 1746, 2008, 2138
RsaNI GTAC 7 cut(s) 37, 613, 1047, 1320, 1745, 2007, 2137
RseI CAYNNNNRTG 3 cut(s) 186, 444, 1507
SacI GAGCTC 1 cut(s) 568
SapI GCTCTTC 1 cut(s) 2522
SatI GCNGC 9 cut(s) 312, 750, 1751, 1754, 2038, 2166, 2350, 2370, 2434
Sau3AI GATC 5 cut(s) 1055, 1796, 1879, 2495, 2698
Sau96I GGNCC 4 cut(s) 86, 508, 531, 1571
ScaI AGTACT 1 cut(s) 2138
SchI GAGTC 5 cut(s) 1310, 1595, 1645, 2309, 2716
ScrFI CCNGG 3 cut(s) 1722, 2128, 2254
SduI GDGCHC 1 cut(s) 568
SfaNI GCATC 9 cut(s) 576, 652, 952, 1541, 1880, 2333, 2554, 2576, 2716
SfcI CTRYAG 4 cut(s) 750, 813, 1748, 2342
Sfr274I CTCGAG 1 cut(s) 2941
SfuI TTCGAA 1 cut(s) 1786
SinI GGWCC 4 cut(s) 86, 508, 531, 1571
SlaI CTCGAG 1 cut(s) 2941
SmiMI CAYNNNNRTG 3 cut(s) 186, 444, 1507
SmlI CTYRAG 7 cut(s) 409, 872, 1346, 1988, 2069, 2357, 2941
SmoI CTYRAG 7 cut(s) 409, 872, 1346, 1988, 2069, 2357, 2941
SsiI CCGC 5 cut(s) 83, 1593, 2350, 2434, 2932
SspI AATATT 3 cut(s) 305, 475, 2145
SspMI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
SstI GAGCTC 1 cut(s) 568
StyD4I CCNGG 3 cut(s) 1720, 2126, 2252
StyI CCWWGG 1 cut(s) 2466
TaaI ACNGT 6 cut(s) 917, 1116, 1284, 2006, 2424, 2591
TaiI ACGT 3 cut(s) 1663, 1693, 2416
TaqI TCGA 5 cut(s) 892, 1549, 1786, 2697, 2942
TatI WGTACW 3 cut(s) 36, 1744, 2136
TauI GCSGC 2 cut(s) 2352, 2436
TfiI GAWTC 6 cut(s) 1088, 1481, 2088, 2281, 2546, 2776
TscAI CASTG 5 cut(s) 36, 523, 682, 2577, 2902
TseFI GTSAC 3 cut(s) 1163, 1818, 2591
TseI GCWGC 7 cut(s) 311, 749, 1750, 1753, 2037, 2165, 2369
Tsp45I GTSAC 3 cut(s) 1163, 1818, 2591
TspGWI ACGGA 2 cut(s) 634, 1218
TspRI CASTG 5 cut(s) 36, 523, 682, 2577, 2902
Vha464I CTTAAG 3 cut(s) 872, 1346, 2357
VpaK11BI GGWCC 4 cut(s) 86, 508, 531, 1571
VspI ATTAAT 1 cut(s) 1125
XapI RAATTY 5 cut(s) 345, 671, 1226, 1628, 2557
XbaI TCTAGA 4 cut(s) 500, 694, 1663, 1774
XceI RCATGY 2 cut(s) 185, 990
XcmI CCANNNNNNNNNTGG 4 cut(s) 214, 1247, 2636, 2737
XhoI CTCGAG 1 cut(s) 2941
XmiI GTMKAC 1 cut(s) 1599
XmnI GAANNNNTTC 1 cut(s) 1782
XspI CTAG 6 cut(s) 501, 695, 1536, 1664, 1775, 2150
ZrmI AGTACT 1 cut(s) 2138
Zsp2I ATGCAT 2 cut(s) 988, 2569
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.