pycom13g01550

Vacuolar fusion protein CCZ1 homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0005810
Physical Location & Seq
Forward (+)
1023732 .. 1026836
3105 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1578 bp
ATGGGCTTGTCGTCTGCCACTACTACGGCTACCACTCCAGAGGGAGTGAAATTGTGCATATTCGATTTGAGGAGGGGTCAGAACGAAGGGCAGGAGTTGGACAAGATACTCTTCTTCTTTCCAGCTGATTTGCCCTTCTCCGCCCAATTCTCTGCGATCGGGCTCAGTGAAGGACTCATCACATTTACAAGAATCTTCTCTCCGGAGGCTGCTTGCGAGGCCATTGAAGCAGAGAGGCATTCCCATGTCTTTTACGAGGCGGAACCCGATATCTGGATGGTCATGGTAGTGGAGAAAAACAAGGAGTTTGAACCGATTTGGAGAAGTGATGCATTGAGGAAGGTTCTCAAGGAAGTACATTCTCTTTTCGTGATGTTTCATGGATCGATAAGAGCGTTGCTTGATAAAGACCCCGGTGGAGGACTAACTCGACCGCATTTGTACCATTTCATCATGGACTATCTCGGTGCATTTCAAAAGCGGTCTCCATTCGATGAGTGTTGCTGGGATTTTCTGGTTGGGAAGAAACTACTGCTACCCTCATTCCGTGACTCTTTGAAGGAGCGTGGAACTGTACAGATGCTTACTGTAGGGCGGGAAGCAGCAATCGAAGTTCAGTCGCTTGTTAGGGTACTTGAATCTTGTGCTGGGAACATGCCGTGTCACTCATTAATCTTGTTTCAGGACCTGTTGGTGTCCACCACACTTTCTCCTGATGATACCGTAAACCTCTTTGCATATGCTGTTTTAAGGTTGACTCCCCGTGCTTTATCCTCTGGAGTAAGTTCCTGGTCCTATTTACGGAAGGGAGCTACATCCTCAGGTTCTAGTTTGGCCCGTCCTGATGCTGTTCCAGAACAATTGCATGGCTCACTTGACAACTCTCCTACTGGAGATAACAGTTCTCGTGTCGTACGGCCCTTGCAACCTGATAAGTGGTCCAAAGGAAAGGACGGTTTTCTTGTCAGTGATATTTGGGGTGCAGAGACTAGTACTGGAGCTTCTGCCACCCCAACAGTTTTGCTTCATCAGACAGAGGAGAGAATGTATCTGTGTACCCATCAGCATAAGAGTCTCACCTTAATTTTTCTCCTTCCTGTTTCTACGGTACTAAATGGCGATCAAGGTATTTCTGCAGTGAAGCAGCAAGTTCTTGAAAATGTATCCCTAAAGCTGTTGAAAGTTGAAGAGAAATTATCAAAGGGATGGGGTGGTGAGAATGCGTATCATGTTAGTGGATATCGGTATTTACTAGTGGATGGAGACAGAAGTGTATCCAGGGCTTCTCCACCAGGAAAAGTCACTACCCTTACCAAGGACTCCTTACTTGCCTTAAGTAAGGTCAGAGAAGAAGTTGATTTGGAAAAAAGTAGAACGAAATCGGATAATGCTGGTCACGAGAAAGAATTGGAAGTTAGCATCAGAGCAAAAAACAATGCTTGGGTTATTGCTCGGGTTACAAGAGGGAAGGAGCTTTATATGGTTTTAGAGAAAGCCAATGAAACGCTTCTCTATGCCTCTGATGCTGTTGAGAAGTTCAGCAACAGGTACTGCAATGGAGCTTTCTCGTTGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0006810 GO:0008150 GO:0016192 GO:0031982 GO:0043226 GO:0043227 GO:0051179 GO:0051234
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1712

Protein Analysis

526

Amino Acids

58.21

Weight (kDa)

6.38

Isoelectric Point (pI)

39.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Intu_longin_1 PF19031 19 - 128 2.1e-19 First Longin domain of INTU, CCZ1 and HPS4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 202
AciI CCGC 5 cut(s) 141, 260, 434, 481, 595
AclWI GGATC 1 cut(s) 391
AfaI GTAC 9 cut(s) 357, 443, 576, 633, 915, 994, 1057, 1110, 1550
AfiI CCNNNNNNNGG 4 cut(s) 273, 419, 801, 1315
AflII CTTAAG 1 cut(s) 1333
AgsI TTSAA 9 cut(s) 227, 311, 476, 559, 638, 1157, 1180, 1187, 1573
AhlI ACTAGT 2 cut(s) 989, 1252
AjnI CCWGG 3 cut(s) 788, 1277, 1291
AluBI AGCT 6 cut(s) 125, 812, 1001, 1174, 1474, 1562
AluI AGCT 6 cut(s) 125, 812, 1001, 1174, 1474, 1562
Alw26I GTCTC 4 cut(s) 489, 980, 1079, 1257
AlwI GGATC 1 cut(s) 391
AlwNI CAGNNNCTG 2 cut(s) 688, 1551
Ama87I CYCGRG 1 cut(s) 1452
Aor13HI TCCGGA 1 cut(s) 202
AoxI GGCC 3 cut(s) 219, 834, 917
ApeKI GCWGC 3 cut(s) 209, 602, 1144
AseI ATTAAT 1 cut(s) 671
Asp700I GAANNNNTTC 1 cut(s) 1506
AspS9I GGNCC 5 cut(s) 685, 792, 835, 918, 939
AsuC2I CCSGG 1 cut(s) 414
AsuHPI GGTGA 2 cut(s) 1069, 1226
AvaI CYCGRG 1 cut(s) 1452
AvaII GGWCC 3 cut(s) 685, 792, 939
AxyI CCTNAGG 1 cut(s) 820
BanII GRGCYC 1 cut(s) 165
BarI GAAGNNNNNNTAC 2 cut(s) 985, 1017
BauI CACGAG 2 cut(s) 906, 1397
BbvI GCAGC 3 cut(s) 196, 614, 1156
BccI CCATC 4 cut(s) 271, 1068, 1200, 1253
BceAI ACGGC 3 cut(s) 42, 643, 932
BciT130I CCWGG 3 cut(s) 790, 1279, 1293
BciVI GTATCC 2 cut(s) 1174, 1285
BcnI CCSGG 1 cut(s) 414
BcoDI GTCTC 4 cut(s) 489, 980, 1079, 1257
BcuI ACTAGT 2 cut(s) 989, 1252
BfaI CTAG 3 cut(s) 828, 990, 1253
BfmI CTRYAG 2 cut(s) 588, 1134
BfrI CTTAAG 1 cut(s) 1333
BfuI GTATCC 2 cut(s) 1174, 1285
BisI GCNGC 3 cut(s) 210, 603, 1145
BlsI GCNGC 3 cut(s) 211, 604, 1146
BmcAI AGTACT 1 cut(s) 994
Bme1390I CCNGG 4 cut(s) 414, 790, 1279, 1293
Bme18I GGWCC 3 cut(s) 685, 792, 939
BmeT110I CYCGRG 1 cut(s) 1452
BmgT120I GGNCC 5 cut(s) 685, 792, 835, 918, 939
BmiI GGNNCC 1 cut(s) 264
BmrFI CCNGG 4 cut(s) 414, 790, 1279, 1293
BmsI GCATC 5 cut(s) 319, 570, 835, 1428, 1513
BpmI CTGGAG 4 cut(s) 21, 798, 912, 1017
BpuEI CTTGAG 1 cut(s) 332
BpuMI CCSGG 1 cut(s) 414
Bsa29I ATCGAT 1 cut(s) 386
BsaI GGTCTC 1 cut(s) 489
BsaJI CCNNGG 3 cut(s) 412, 1278, 1314
BsaWI WCCGGW 1 cut(s) 202
BsaXI ACNNNNNCTCC 4 cut(s) 694, 724, 1031, 1061
Bsc4I CCNNNNNNNGG 4 cut(s) 273, 419, 801, 1315
Bse1I ACTGG 2 cut(s) 895, 1000
Bse21I CCTNAGG 1 cut(s) 820
Bse3DI GCAATG 1 cut(s) 1561
BseAI TCCGGA 1 cut(s) 202
BseBI CCWGG 3 cut(s) 790, 1279, 1293
BseCI ATCGAT 1 cut(s) 386
BseDI CCNNGG 3 cut(s) 412, 1278, 1314
BseGI GGATG 4 cut(s) 282, 815, 1211, 1264
BseLI CCNNNNNNNGG 4 cut(s) 273, 419, 801, 1315
BseMI GCAATG 1 cut(s) 1561
BseMII CTCAG 2 cut(s) 178, 834
BseNI ACTGG 2 cut(s) 895, 1000
BseRI GAGGAG 2 cut(s) 85, 1052
BseXI GCAGC 3 cut(s) 196, 614, 1156
BseYI CCCAGC 2 cut(s) 504, 647
BsgI GTGCAG 1 cut(s) 1002
Bsh1285I CGRYCG 2 cut(s) 159, 434
BshFI GGCC 3 cut(s) 221, 836, 919
BshVI ATCGAT 1 cut(s) 386
BsiEI CGRYCG 2 cut(s) 159, 434
BsiHKCI CYCGRG 1 cut(s) 1452
BsiSI CCGG 2 cut(s) 203, 414
BsiWI CGTACG 1 cut(s) 913
BslI CCNNNNNNNGG 4 cut(s) 273, 419, 801, 1315
BsmAI GTCTC 4 cut(s) 489, 980, 1079, 1257
BsmI GAATGC 2 cut(s) 238, 1225
BsnI GGCC 3 cut(s) 221, 836, 919
Bso31I GGTCTC 1 cut(s) 489
BsoBI CYCGRG 1 cut(s) 1452
Bsp1286I GDGCHC 1 cut(s) 165
Bsp13I TCCGGA 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 574
Bsp143I GATC 3 cut(s) 156, 383, 1120
BspACI CCGC 5 cut(s) 141, 260, 434, 481, 595
BspANI GGCC 3 cut(s) 221, 836, 919
BspCNI CTCAG 2 cut(s) 177, 833
BspDI ATCGAT 1 cut(s) 386
BspEI TCCGGA 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 264
BspMAI CTGCAG 1 cut(s) 1138
BspPI GGATC 1 cut(s) 391
BspTI CTTAAG 1 cut(s) 1333
BspTNI GGTCTC 1 cut(s) 489
BsrDI GCAATG 1 cut(s) 1561
BsrGI TGTACA 1 cut(s) 574
BsrI ACTGG 2 cut(s) 895, 1000
BssECI CCNNGG 3 cut(s) 412, 1278, 1314
BssMI GATC 3 cut(s) 156, 383, 1120
BssSI CACGAG 2 cut(s) 906, 1397
BssT1I CCWWGG 1 cut(s) 1314
Bst2BI CACGAG 2 cut(s) 906, 1397
Bst2UI CCWGG 3 cut(s) 790, 1279, 1293
Bst4CI ACNGT 7 cut(s) 574, 589, 724, 902, 956, 1018, 1108
Bst6I CTCTTC 2 cut(s) 116, 1182
BstAFI CTTAAG 1 cut(s) 1333
BstAUI TGTACA 1 cut(s) 574
BstC8I GCNNGC 1 cut(s) 214
BstDEI CTNAG 2 cut(s) 164, 820
BstENI CCTNNNNNAGG 1 cut(s) 1313
BstF5I GGATG 4 cut(s) 282, 815, 1211, 1264
BstKTI GATC 3 cut(s) 159, 386, 1123
BstMAI GTCTC 4 cut(s) 489, 980, 1079, 1257
BstMBI GATC 3 cut(s) 156, 383, 1120
BstMCI CGRYCG 2 cut(s) 159, 434
BstMWI GCNNNNNNNGC 3 cut(s) 218, 227, 1523
BstNI CCWGG 3 cut(s) 790, 1279, 1293
BstNSI RCATGY 1 cut(s) 658
BstSCI CCNGG 4 cut(s) 412, 788, 1277, 1291
BstSFI CTRYAG 2 cut(s) 588, 1134
BstV1I GCAGC 3 cut(s) 196, 614, 1156
Bsu15I ATCGAT 1 cut(s) 386
Bsu36I CCTNAGG 1 cut(s) 820
BsuI GTATCC 2 cut(s) 1174, 1285
BsuRI GGCC 3 cut(s) 221, 836, 919
BsuTUI ATCGAT 1 cut(s) 386
BtsCI GGATG 4 cut(s) 282, 815, 1211, 1264
BtsI GCAGTG 1 cut(s) 1143
BtsIMutI CAGTG 3 cut(s) 172, 973, 1143
Cac8I GCNNGC 1 cut(s) 214
CaiI CAGNNNCTG 2 cut(s) 688, 1551
Cfr13I GGNCC 5 cut(s) 685, 792, 835, 918, 939
ClaI ATCGAT 1 cut(s) 386
Csp6I GTAC 9 cut(s) 356, 442, 575, 632, 914, 993, 1056, 1109, 1549
CviAII CATG 7 cut(s) 245, 283, 380, 454, 655, 866, 1229
CviQI GTAC 9 cut(s) 356, 442, 575, 632, 914, 993, 1056, 1109, 1549
DdeI CTNAG 2 cut(s) 164, 820
DpnI GATC 3 cut(s) 158, 385, 1122
DpnII GATC 3 cut(s) 156, 383, 1120
Eam1104I CTCTTC 2 cut(s) 116, 1182
EarI CTCTTC 2 cut(s) 116, 1182
EciI GGCGGA 2 cut(s) 130, 275
Eco130I CCWWGG 1 cut(s) 1314
Eco24I GRGCYC 1 cut(s) 165
Eco31I GGTCTC 1 cut(s) 489
Eco32I GATATC 2 cut(s) 271, 1241
Eco47I GGWCC 3 cut(s) 685, 792, 939
Eco81I CCTNAGG 1 cut(s) 820
Eco88I CYCGRG 1 cut(s) 1452
EcoNI CCTNNNNNAGG 1 cut(s) 1313
EcoO109I RGGNCCY 1 cut(s) 685
EcoRII CCWGG 3 cut(s) 788, 1277, 1291
EcoRV GATATC 2 cut(s) 271, 1241
EcoT14I CCWWGG 1 cut(s) 1314
EcoT22I ATGCAT 1 cut(s) 334
EcoT38I GRGCYC 1 cut(s) 165
ErhI CCWWGG 1 cut(s) 1314
FaeI CATG 7 cut(s) 248, 286, 383, 457, 658, 869, 1232
FalI AAGNNNNNCTT 4 cut(s) 95, 127, 1307, 1339
FatI CATG 7 cut(s) 244, 282, 379, 453, 654, 865, 1228
FauI CCCGC 1 cut(s) 588
FauNDI CATATG 1 cut(s) 739
Fnu4HI GCNGC 3 cut(s) 210, 603, 1145
FokI GGATG 4 cut(s) 289, 802, 1218, 1271
FriOI GRGCYC 1 cut(s) 165
Fsp4HI GCNGC 3 cut(s) 210, 603, 1145
FspBI CTAG 3 cut(s) 828, 990, 1253
GluI GCNGC 3 cut(s) 210, 603, 1145
GsaI CCCAGC 2 cut(s) 508, 651
GsuI CTGGAG 4 cut(s) 21, 798, 912, 1017
HaeIII GGCC 3 cut(s) 221, 836, 919
HapII CCGG 2 cut(s) 203, 414
Hin1II CATG 7 cut(s) 248, 286, 383, 457, 658, 869, 1232
HincII GTYRAC 1 cut(s) 756
HindII GTYRAC 1 cut(s) 756
HinfI GANTC 7 cut(s) 174, 192, 551, 638, 757, 1072, 1319
HpaII CCGG 2 cut(s) 203, 414
HphI GGTGA 2 cut(s) 1069, 1226
Hpy166II GTNNAC 4 cut(s) 699, 727, 756, 1056
Hpy188I TCNGA 6 cut(s) 81, 1032, 1346, 1384, 1424, 1522
Hpy8I GTNNAC 4 cut(s) 699, 727, 756, 1056
HpyAV CCTTC 8 cut(s) 80, 145, 164, 334, 553, 799, 1103, 1462
HpyCH4III ACNGT 7 cut(s) 574, 589, 724, 902, 956, 1018, 1108
HpyCH4V TGCA 9 cut(s) 57, 332, 470, 737, 865, 925, 983, 1136, 1554
HpyF10VI GCNNNNNNNGC 3 cut(s) 218, 227, 1523
HpyF3I CTNAG 2 cut(s) 164, 820
Hsp92II CATG 7 cut(s) 248, 286, 383, 457, 658, 869, 1232
Kpn2I TCCGGA 1 cut(s) 202
Kzo9I GATC 3 cut(s) 156, 383, 1120
LmnI GCTCC 5 cut(s) 562, 809, 998, 1471, 1559
Lsp1109I GCAGC 3 cut(s) 196, 614, 1156
LweI GCATC 5 cut(s) 319, 570, 835, 1428, 1513
MaeI CTAG 3 cut(s) 828, 990, 1253
MaeIII GTNAC 5 cut(s) 548, 662, 1300, 1394, 1456
MalI GATC 3 cut(s) 158, 385, 1122
MboI GATC 3 cut(s) 156, 383, 1120
MboII GAAGA 6 cut(s) 103, 106, 187, 535, 1199, 1361
MfeI CAATTG 1 cut(s) 860
MhlI GDGCHC 1 cut(s) 165
MluCI AATT 7 cut(s) 50, 146, 860, 1083, 1193, 1406, 1573
MlyI GAGTC 5 cut(s) 168, 545, 751, 1081, 1313
MmeI TCCRAC 1 cut(s) 78
Mph1103I ATGCAT 1 cut(s) 334
MroI TCCGGA 1 cut(s) 202
MroXI GAANNNNTTC 1 cut(s) 1506
MseI TTAA 4 cut(s) 671, 749, 1082, 1334
MslI CAYNNNNRTG 3 cut(s) 243, 287, 1233
MspA1I CMGCKG 1 cut(s) 125
MspCI CTTAAG 1 cut(s) 1333
MspI CCGG 2 cut(s) 203, 414
MspR9I CCNGG 4 cut(s) 414, 790, 1279, 1293
MunI CAATTG 1 cut(s) 860
Mva1269I GAATGC 2 cut(s) 238, 1225
MvaI CCWGG 3 cut(s) 790, 1279, 1293
MwoI GCNNNNNNNGC 3 cut(s) 218, 227, 1523
NciI CCSGG 1 cut(s) 414
NdeI CATATG 1 cut(s) 739
NdeII GATC 3 cut(s) 156, 383, 1120
NlaIII CATG 7 cut(s) 248, 286, 383, 457, 658, 869, 1232
NlaIV GGNNCC 1 cut(s) 264
NmuCI GTSAC 4 cut(s) 548, 662, 1300, 1394
NsiI ATGCAT 1 cut(s) 334
NspI RCATGY 1 cut(s) 658
PcsI WCGNNNNNNNCGW 1 cut(s) 392
PctI GAATGC 2 cut(s) 238, 1225
PdmI GAANNNNTTC 1 cut(s) 1506
PfeI GAWTC 2 cut(s) 192, 638
Pfl23II CGTACG 1 cut(s) 913
PkrI GCNGC 3 cut(s) 211, 604, 1146
Ple19I CGATCG 1 cut(s) 159
PleI GAGTC 5 cut(s) 168, 545, 751, 1080, 1313
PpsI GAGTC 5 cut(s) 168, 545, 751, 1080, 1313
PpuMI RGGWCCY 1 cut(s) 685
PshBI ATTAAT 1 cut(s) 671
Psp5II RGGWCCY 1 cut(s) 685
Psp6I CCWGG 3 cut(s) 788, 1277, 1291
PspFI CCCAGC 2 cut(s) 504, 647
PspGI CCWGG 3 cut(s) 788, 1277, 1291
PspLI CGTACG 1 cut(s) 913
PspN4I GGNNCC 1 cut(s) 264
PspPI GGNCC 5 cut(s) 685, 792, 835, 918, 939
PspPPI RGGWCCY 1 cut(s) 685
PstI CTGCAG 1 cut(s) 1138
PstNI CAGNNNCTG 2 cut(s) 688, 1551
PvuI CGATCG 1 cut(s) 159
PvuII CAGCTG 1 cut(s) 125
RsaI GTAC 9 cut(s) 357, 443, 576, 633, 915, 994, 1057, 1110, 1550
RsaNI GTAC 9 cut(s) 356, 442, 575, 632, 914, 993, 1056, 1109, 1549
RseI CAYNNNNRTG 3 cut(s) 243, 287, 1233
SaqAI TTAA 4 cut(s) 671, 749, 1082, 1334
SatI GCNGC 3 cut(s) 210, 603, 1145
Sau3AI GATC 3 cut(s) 156, 383, 1120
Sau96I GGNCC 5 cut(s) 685, 792, 835, 918, 939
ScaI AGTACT 1 cut(s) 994
SchI GAGTC 5 cut(s) 168, 545, 751, 1081, 1313
ScrFI CCNGG 4 cut(s) 414, 790, 1279, 1293
SduI GDGCHC 1 cut(s) 165
SfaNI GCATC 5 cut(s) 319, 570, 835, 1428, 1513
SfcI CTRYAG 2 cut(s) 588, 1134
SinI GGWCC 3 cut(s) 685, 792, 939
SmiMI CAYNNNNRTG 3 cut(s) 243, 287, 1233
SmlI CTYRAG 2 cut(s) 347, 1333
SmoI CTYRAG 2 cut(s) 347, 1333
SpeI ACTAGT 2 cut(s) 989, 1252
Sse9I AATT 7 cut(s) 50, 146, 860, 1083, 1193, 1406, 1573
SsiI CCGC 5 cut(s) 141, 260, 434, 481, 595
SspMI CTAG 3 cut(s) 828, 990, 1253
StyD4I CCNGG 4 cut(s) 412, 788, 1277, 1291
StyI CCWWGG 1 cut(s) 1314
TaaI ACNGT 7 cut(s) 574, 589, 724, 902, 956, 1018, 1108
TaqI TCGA 5 cut(s) 63, 386, 430, 492, 609
TasI AATT 7 cut(s) 50, 146, 860, 1083, 1193, 1406, 1573
TatI WGTACW 3 cut(s) 355, 574, 992
TfiI GAWTC 2 cut(s) 192, 638
Tru1I TTAA 4 cut(s) 671, 749, 1082, 1334
Tru9I TTAA 4 cut(s) 671, 749, 1082, 1334
TscAI CASTG 3 cut(s) 172, 973, 1143
TseFI GTSAC 4 cut(s) 548, 662, 1300, 1394
TseI GCWGC 3 cut(s) 209, 602, 1144
Tsp45I GTSAC 4 cut(s) 548, 662, 1300, 1394
TspDTI ATGAA 4 cut(s) 368, 439, 1016, 1515
TspGWI ACGGA 2 cut(s) 536, 817
TspRI CASTG 3 cut(s) 172, 973, 1143
Vha464I CTTAAG 1 cut(s) 1333
VpaK11BI GGWCC 3 cut(s) 685, 792, 939
VspI ATTAAT 1 cut(s) 671
XagI CCTNNNNNAGG 1 cut(s) 1313
XceI RCATGY 1 cut(s) 658
XmnI GAANNNNTTC 1 cut(s) 1506
XspI CTAG 3 cut(s) 828, 990, 1253
ZrmI AGTACT 1 cut(s) 994
Zsp2I ATGCAT 1 cut(s) 334
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.