pycom13g02080

Uncharacterized protein At4g22758-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014102
Physical Location & Seq
Reverse (-)
1401786 .. 1402361
576 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 294 bp
ATGGACACCGCATTAAAATCCTATGCGCGTGAGGGCCGCTTCCCGGTTCTTGGCTCCGATCTCAATGACTTCCTGCTCTACTGCCCCGCCGTAGGACCTGAAGCTCTGAGCCCGTGGGACACAATCGGAGCGCAAGGAGCTCGTAACTTTATGCTGTGCAAGAAGCCTCAGCCAGTGAAGATGGAGAAGGATGAGATACCAGCAGCTGCAATTACTCGCAAGGGTAGTGGGAGCTGGAAGGCTTCTCAGAGATGGATCAATAAGTCCCTCAATCTCAAGGTCTCTTCCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

98

Amino Acids

10.69

Weight (kDa)

9.47

Isoelectric Point (pI)

40.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF7054 PF23156 2 - 53 6e-15 Domain of unknown function (DUF7054)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014102)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G23150 AT1G70780
fragaria_vesca FvH4_4g32830
malus_domestica MD13G1022300.v1.1 MD16G1023400.v1.1
prunus_persica Prupe.1G329500_v2.0.a1
pyrus_communis pycom13g02080
rosa_chinensis RchiOBHm_Chr4g0441841
rosa_laevigata RLG00000006061
rosa_multiflora Rmu_sc0008316.1_g000006
rosa_roxburghii Rroxscaffold_5G00382670
rosa_rugosa Rorug04G0336600
rosa_samantha Rh4AG388000 Rh4BG401700 Rh4CG416700 Rh4DG395100
rosa_wichuraiana Rw4G033500

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 28
AciI CCGC 3 cut(s) 9, 37, 87
AclWI GGATC 1 cut(s) 263
AcuI CTGAAG 1 cut(s) 120
AfiI CCNNNNNNNGG 3 cut(s) 43, 50, 92
AluBI AGCT 4 cut(s) 104, 140, 206, 234
AluI AGCT 4 cut(s) 104, 140, 206, 234
Alw21I GWGCWC 1 cut(s) 142
Alw26I GTCTC 1 cut(s) 286
AlwI GGATC 1 cut(s) 263
AlwNI CAGNNNCTG 1 cut(s) 206
AoxI GGCC 1 cut(s) 34
ApeKI GCWGC 2 cut(s) 203, 206
AspLEI GCGC 2 cut(s) 28, 133
AspS9I GGNCC 2 cut(s) 34, 95
AsuC2I CCSGG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 95
BanII GRGCYC 2 cut(s) 113, 142
Bbv12I GWGCWC 1 cut(s) 142
BbvCI CCTCAGC 1 cut(s) 168
BbvI GCAGC 2 cut(s) 193, 215
BccI CCATC 2 cut(s) 175, 246
BceAI ACGGC 1 cut(s) 74
BcgI CGANNNNNNTGC 2 cut(s) 122, 156
BcnI CCSGG 1 cut(s) 44
BcoDI GTCTC 1 cut(s) 286
BisI GCNGC 3 cut(s) 37, 204, 207
BlsI GCNGC 3 cut(s) 38, 205, 208
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 1 cut(s) 95
BmgT120I GGNCC 2 cut(s) 34, 95
BmiI GGNNCC 1 cut(s) 55
BmrFI CCNGG 1 cut(s) 44
Bpu10I CCTNAGC 1 cut(s) 168
BpuEI CTTGAG 1 cut(s) 260
BpuMI CCSGG 1 cut(s) 44
BsaI GGTCTC 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 3 cut(s) 43, 50, 92
Bse1I ACTGG 1 cut(s) 173
BseDI CCNNGG 1 cut(s) 113
BseGI GGATG 1 cut(s) 196
BseLI CCNNNNNNNGG 3 cut(s) 43, 50, 92
BseMII CTCAG 3 cut(s) 98, 182, 260
BseNI ACTGG 1 cut(s) 173
BseXI GCAGC 2 cut(s) 193, 215
Bsh1236I CGCG 1 cut(s) 28
BshFI GGCC 1 cut(s) 36
BsiHKAI GWGCWC 1 cut(s) 142
BsiSI CCGG 1 cut(s) 44
BslFI GGGAC 2 cut(s) 131, 250
BslI CCNNNNNNNGG 3 cut(s) 43, 50, 92
BsmAI GTCTC 1 cut(s) 286
BsmFI GGGAC 2 cut(s) 131, 250
BsnI GGCC 1 cut(s) 36
Bso31I GGTCTC 1 cut(s) 286
Bsp1286I GDGCHC 2 cut(s) 113, 142
Bsp143I GATC 2 cut(s) 58, 255
BspACI CCGC 3 cut(s) 9, 37, 87
BspANI GGCC 1 cut(s) 36
BspCNI CTCAG 3 cut(s) 99, 181, 259
BspFNI CGCG 1 cut(s) 28
BspLI GGNNCC 1 cut(s) 55
BspPI GGATC 1 cut(s) 263
BspTNI GGTCTC 1 cut(s) 286
BsrI ACTGG 1 cut(s) 173
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 2 cut(s) 58, 255
Bst6I CTCTTC 1 cut(s) 289
BstDEI CTNAG 3 cut(s) 107, 168, 246
BstDSI CCRYGG 1 cut(s) 113
BstF5I GGATG 1 cut(s) 196
BstFNI CGCG 1 cut(s) 28
BstHHI GCGC 2 cut(s) 28, 133
BstKTI GATC 2 cut(s) 61, 258
BstMAI GTCTC 1 cut(s) 286
BstMBI GATC 2 cut(s) 58, 255
BstMWI GCNNNNNNNGC 1 cut(s) 137
BstSCI CCNGG 1 cut(s) 42
BstUI CGCG 1 cut(s) 28
BstV1I GCAGC 2 cut(s) 193, 215
BsuRI GGCC 1 cut(s) 36
BtgI CCRYGG 1 cut(s) 113
BtsCI GGATG 1 cut(s) 196
BtsIMutI CAGTG 1 cut(s) 180
CaiI CAGNNNCTG 1 cut(s) 206
CfoI GCGC 2 cut(s) 28, 133
Cfr13I GGNCC 2 cut(s) 34, 95
CspCI CAANNNNNGTGG 2 cut(s) 208, 243
DdeI CTNAG 3 cut(s) 107, 168, 246
DpnI GATC 2 cut(s) 60, 257
DpnII GATC 2 cut(s) 58, 255
Eam1104I CTCTTC 1 cut(s) 289
EarI CTCTTC 1 cut(s) 289
Ecl136II GAGCTC 1 cut(s) 140
Eco24I GRGCYC 2 cut(s) 113, 142
Eco31I GGTCTC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 95
Eco53kI GAGCTC 1 cut(s) 140
Eco57I CTGAAG 1 cut(s) 120
EcoICRI GAGCTC 1 cut(s) 140
EcoO109I RGGNCCY 1 cut(s) 95
EcoT38I GRGCYC 2 cut(s) 113, 142
FaiI YATR 2 cut(s) 24, 152
FaqI GGGAC 2 cut(s) 131, 250
FauI CCCGC 1 cut(s) 94
Fnu4HI GCNGC 3 cut(s) 37, 204, 207
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 2 cut(s) 113, 142
Fsp4HI GCNGC 3 cut(s) 37, 204, 207
GlaI GCGC 2 cut(s) 27, 132
GluI GCNGC 3 cut(s) 37, 204, 207
HaeIII GGCC 1 cut(s) 36
HapII CCGG 1 cut(s) 44
HhaI GCGC 2 cut(s) 28, 133
Hin6I GCGC 2 cut(s) 26, 131
HinP1I GCGC 2 cut(s) 26, 131
HpaII CCGG 1 cut(s) 44
Hpy188I TCNGA 4 cut(s) 58, 108, 128, 249
HpyAV CCTTC 2 cut(s) 181, 232
HpyCH4V TGCA 2 cut(s) 159, 209
HpyF10VI GCNNNNNNNGC 1 cut(s) 137
HpyF3I CTNAG 3 cut(s) 107, 168, 246
HspAI GCGC 2 cut(s) 26, 131
Kzo9I GATC 2 cut(s) 58, 255
LmnI GCTCC 4 cut(s) 59, 128, 137, 231
LpnPI CCDG 6 cut(s) 57, 86, 111, 186, 213, 220
Lsp1109I GCAGC 2 cut(s) 193, 215
MaeIII GTNAC 1 cut(s) 143
MalI GATC 2 cut(s) 60, 257
MboI GATC 2 cut(s) 58, 255
MboII GAAGA 2 cut(s) 190, 276
MhlI GDGCHC 2 cut(s) 113, 142
MluCI AATT 1 cut(s) 210
MnlI CCTC 3 cut(s) 25, 177, 278
MseI TTAA 2 cut(s) 14, 292
MspA1I CMGCKG 1 cut(s) 206
MspI CCGG 1 cut(s) 44
MspR9I CCNGG 1 cut(s) 44
MvnI CGCG 1 cut(s) 28
MwoI GCNNNNNNNGC 1 cut(s) 137
NciI CCSGG 1 cut(s) 44
NdeII GATC 2 cut(s) 58, 255
NlaIV GGNNCC 1 cut(s) 55
PkrI GCNGC 3 cut(s) 38, 205, 208
PpuMI RGGWCCY 1 cut(s) 95
Psp124BI GAGCTC 1 cut(s) 142
Psp5II RGGWCCY 1 cut(s) 95
PspN4I GGNNCC 1 cut(s) 55
PspPI GGNCC 2 cut(s) 34, 95
PspPPI RGGWCCY 1 cut(s) 95
PstNI CAGNNNCTG 1 cut(s) 206
PvuII CAGCTG 1 cut(s) 206
SacI GAGCTC 1 cut(s) 142
SaqAI TTAA 2 cut(s) 14, 292
SatI GCNGC 3 cut(s) 37, 204, 207
Sau3AI GATC 2 cut(s) 58, 255
Sau96I GGNCC 2 cut(s) 34, 95
ScrFI CCNGG 1 cut(s) 44
SduI GDGCHC 2 cut(s) 113, 142
SetI ASST 6 cut(s) 100, 106, 142, 208, 236, 282
SinI GGWCC 1 cut(s) 95
SmlI CTYRAG 1 cut(s) 275
SmoI CTYRAG 1 cut(s) 275
Sse9I AATT 1 cut(s) 210
SsiI CCGC 3 cut(s) 9, 37, 87
SstI GAGCTC 1 cut(s) 142
StyD4I CCNGG 1 cut(s) 42
TasI AATT 1 cut(s) 210
TauI GCSGC 1 cut(s) 39
Tru1I TTAA 2 cut(s) 14, 292
Tru9I TTAA 2 cut(s) 14, 292
TscAI CASTG 1 cut(s) 180
TseI GCWGC 2 cut(s) 203, 206
TspRI CASTG 1 cut(s) 180
VpaK11BI GGWCC 1 cut(s) 95
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.