pycom13g05020

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0014498
Physical Location & Seq
Reverse (-)
3361934 .. 3363843
1910 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1242 bp
ATGGACATGTTTGATGGACATCTGGTCCTTTCTCTCAGCAAGAAGGGGTTTTCTATGTTGTGTTCCATTAATTTGCCTGTCCTTGTTGATTGTAAGCACCAATTTGAGATTGATGATCTTGATCCATGGTTTTTCGCTCTGCCCTCAAACTTATGTAATGTTGTTCCAGGTTCTAACCATGATTTCCATAACTCAGTATACCGTGCAGTGCTTTCATCTCCAGTGATGCCTGATGTAGTTGTTGACTACGACATGTCGAGCAGGAGATTCTCAATTGTGCAGCAAGAGGAAGTGAGGGATTTTTATGATAGAACATTCCCACCAACTAATCAGCTCGATTTGAATAAAAAATTTGGCTCACAATATCAGGAGGATGTCCAGATAAGTGAATCACAGAGATGGAAGGAGTATTCTGATGAATACTGCTGTGTGAGGAAGGAGGTTGTCTCCCATGATGGTGTTAGAGTTCCGTTGACCATATTGTACTCTCATACAGCCTGGGATAAGAGTCAGTCGCCTGGCCTTCTGAAAGGCTATGGAGCATATGGGGAGGTTTTAGATAAAAGCTGGTGTGCAGAGCACCTGTGTTTACTTGATCGGGGATGGGTTGTGGCATTTGCTGATGTGAGGGGTGGTGGTGGTGTAGATTCTTCGTGGCACAAATCTGGCACTGGGTTTAATAAATTGAATTCAATATATGATTTTGTATCATGTGGAAACTACCTGATTGAAGAGGGATACGTACATAAAGATCGGCTTGGTGCTATTGGACATAGTGCTGGAGGCCTTCTTGTTGGAGCAACTATCAATATGTATCCCGATCTCTTTCGAGCTTCTATTTTGAAGGTTCCGTTCCTTGATATATGCAACACTTTAATGGATCCTAGTTTGCCTCTCACCATTCTGGATTATGAAGAATTCGGCAATCCTCAGATACAGTCCGAATTTGAGCTTATCTTCAGTTATTCACCGTACGATAACATTTCTCAAGGCAGTTGCTACCCTTCAATGCTTGTTACAGCGTCCCTTCACGACTCAAGGGTTGGGTTTTGGGAAGCCGCCAAGTGGGTGGCCAAAGTTCGAGAGAGTACATGTCCTCGTTGTTCATGTTCAGTCATTCTGAAGACAAATATGGCTGGAGGACATTTTGGCGAAGGTGGACGGTATGCTCAATGTGAGGAAGCTGCTTATGATTATGCATTTCTATTCAAAGCTATGGGGTTGCTGAAGATTGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

414

Amino Acids

46.41

Weight (kDa)

5.17

Isoelectric Point (pI)

46.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9 PF00326 197 - 407 3.4e-45 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69020 AT1G69020
fragaria_vesca FvH4_4g29260
malus_domestica MD13G1056200.v1.1
prunus_persica Prupe.1G293900_v2.0.a1
pyrus_communis pycom13g05020
rosa_chinensis RchiOBHm_Chr4g0437311
rosa_laevigata RLG00000006447
rosa_multiflora Rmu_co8522681.1_g000001 Rmu_sc0004339.1_g000034
rosa_roxburghii Rroxscaffold_5G00378460
rosa_rugosa Rorug04G0299500
rosa_samantha Rh4AG354500 Rh4BG364300 Rh4CG378600 Rh4DG358200
rosa_wichuraiana Rw4G031080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 198
AciI CCGC 1 cut(s) 1059
AclWI GGATC 3 cut(s) 116, 875, 888
AcoI YGGCCR 1 cut(s) 1071
AcsI RAATTY 4 cut(s) 350, 688, 917, 944
AcuI CTGAAG 2 cut(s) 943, 1142
AfaI GTAC 4 cut(s) 485, 744, 974, 1090
AfiI CCNNNNNNNGG 1 cut(s) 1065
AflIII ACRYGT 3 cut(s) 6, 252, 1091
AgsI TTSAA 8 cut(s) 343, 688, 693, 731, 844, 1008, 1210, 1235
AhdI GACNNNNNGTC 1 cut(s) 23
AjnI CCWGG 3 cut(s) 166, 497, 517
AluBI AGCT 6 cut(s) 334, 567, 833, 952, 1184, 1214
AluI AGCT 6 cut(s) 334, 567, 833, 952, 1184, 1214
Alw21I GWGCWC 1 cut(s) 582
Alw26I GTCTC 1 cut(s) 451
AlwI GGATC 3 cut(s) 116, 875, 888
AoxI GGCC 3 cut(s) 520, 784, 1071
ApeKI GCWGC 2 cut(s) 280, 1184
ApoI RAATTY 4 cut(s) 350, 688, 917, 944
AseI ATTAAT 1 cut(s) 69
AspS9I GGNCC 1 cut(s) 25
AsuHPI GGTGA 2 cut(s) 889, 960
AvaII GGWCC 1 cut(s) 25
BalI TGGCCA 1 cut(s) 1073
BamHI GGATCC 1 cut(s) 880
BbsI GAAGAC 1 cut(s) 1130
Bbv12I GWGCWC 1 cut(s) 582
BbvI GCAGC 2 cut(s) 292, 1171
BccI CCATC 4 cut(s) 8, 393, 449, 597
BciT130I CCWGG 3 cut(s) 168, 499, 519
BciVI GTATCC 2 cut(s) 731, 825
BcoDI GTCTC 1 cut(s) 451
BfaI CTAG 1 cut(s) 885
BfuI GTATCC 2 cut(s) 731, 825
BisI GCNGC 3 cut(s) 281, 1059, 1185
BlsI GCNGC 3 cut(s) 282, 1060, 1186
Bme1390I CCNGG 3 cut(s) 168, 499, 519
Bme18I GGWCC 1 cut(s) 25
BmeRI GACNNNNNGTC 1 cut(s) 23
BmgT120I GGNCC 1 cut(s) 25
BmiI GGNNCC 2 cut(s) 849, 882
BmrFI CCNGG 3 cut(s) 168, 499, 519
BmrI ACTGGG 1 cut(s) 681
BmsI GCATC 1 cut(s) 216
BmuI ACTGGG 1 cut(s) 681
BpiI GAAGAC 1 cut(s) 1130
BplI GAGNNNNNCTC 2 cut(s) 431, 463
BpmI CTGGAG 3 cut(s) 204, 801, 1158
BpuEI CTTGAG 2 cut(s) 972, 1021
BsaAI YACGTR 1 cut(s) 742
BsaBI GATNNNNATC 2 cut(s) 18, 120
BsaJI CCNNGG 2 cut(s) 125, 498
Bsc4I CCNNNNNNNGG 1 cut(s) 1065
Bse1I ACTGG 2 cut(s) 221, 676
Bse8I GATNNNNATC 2 cut(s) 18, 120
BseBI CCWGG 3 cut(s) 168, 499, 519
BseDI CCNNGG 2 cut(s) 125, 498
BseGI GGATG 2 cut(s) 379, 608
BseJI GATNNNNATC 2 cut(s) 18, 120
BseLI CCNNNNNNNGG 1 cut(s) 1065
BseMII CTCAG 3 cut(s) 49, 207, 944
BseNI ACTGG 2 cut(s) 221, 676
BseXI GCAGC 2 cut(s) 292, 1171
BsgI GTGCAG 3 cut(s) 225, 299, 594
BshFI GGCC 3 cut(s) 522, 786, 1073
BsiHKAI GWGCWC 1 cut(s) 582
BsiWI CGTACG 1 cut(s) 972
BslFI GGGAC 1 cut(s) 1009
BslI CCNNNNNNNGG 1 cut(s) 1065
BsmAI GTCTC 1 cut(s) 451
BsmFI GGGAC 1 cut(s) 1009
BsnI GGCC 3 cut(s) 522, 786, 1073
Bsp1286I GDGCHC 1 cut(s) 582
Bsp143I GATC 6 cut(s) 115, 121, 595, 751, 820, 880
Bsp19I CCATGG 1 cut(s) 125
BspACI CCGC 1 cut(s) 1059
BspANI GGCC 3 cut(s) 522, 786, 1073
BspCNI CTCAG 3 cut(s) 48, 206, 943
BspLI GGNNCC 2 cut(s) 849, 882
BspPI GGATC 3 cut(s) 116, 875, 888
BsrI ACTGG 2 cut(s) 221, 676
BssECI CCNNGG 2 cut(s) 125, 498
BssMI GATC 6 cut(s) 115, 121, 595, 751, 820, 880
BssNAI GTATAC 1 cut(s) 199
BssT1I CCWWGG 1 cut(s) 125
Bst1107I GTATAC 1 cut(s) 199
Bst2UI CCWGG 3 cut(s) 168, 499, 519
Bst4CI ACNGT 4 cut(s) 203, 939, 972, 1164
Bst6I CTCTTC 1 cut(s) 726
BstBAI YACGTR 1 cut(s) 742
BstDEI CTNAG 3 cut(s) 35, 193, 930
BstDSI CCRYGG 1 cut(s) 125
BstF5I GGATG 2 cut(s) 379, 608
BstKTI GATC 6 cut(s) 118, 124, 598, 754, 823, 883
BstMAI GTCTC 1 cut(s) 451
BstMBI GATC 6 cut(s) 115, 121, 595, 751, 820, 880
BstNI CCWGG 3 cut(s) 168, 499, 519
BstNSI RCATGY 3 cut(s) 10, 256, 1095
BstSCI CCNGG 3 cut(s) 166, 497, 517
BstSNI TACGTA 1 cut(s) 742
BstV1I GCAGC 2 cut(s) 292, 1171
BstV2I GAAGAC 1 cut(s) 1130
BstX2I RGATCY 1 cut(s) 880
BstXI CCANNNNNNTGG 1 cut(s) 1069
BstYI RGATCY 1 cut(s) 880
BstZ17I GTATAC 1 cut(s) 199
BsuI GTATCC 2 cut(s) 731, 825
BsuRI GGCC 3 cut(s) 522, 786, 1073
BtgI CCRYGG 1 cut(s) 125
BtsCI GGATG 2 cut(s) 379, 608
BtsI GCAGTG 1 cut(s) 213
BtsIMutI CAGTG 3 cut(s) 213, 228, 669
Cfr13I GGNCC 1 cut(s) 25
CseI GACGC 1 cut(s) 1011
Csp6I GTAC 4 cut(s) 484, 743, 973, 1089
CviAII CATG 8 cut(s) 7, 126, 179, 253, 452, 711, 1092, 1107
CviQI GTAC 4 cut(s) 484, 743, 973, 1089
DdeI CTNAG 3 cut(s) 35, 193, 930
DpnI GATC 6 cut(s) 117, 123, 597, 753, 822, 882
DpnII GATC 6 cut(s) 115, 121, 595, 751, 820, 880
DriI GACNNNNNGTC 1 cut(s) 23
EaeI YGGCCR 1 cut(s) 1071
Eam1104I CTCTTC 1 cut(s) 726
Eam1105I GACNNNNNGTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 726
Eco105I TACGTA 1 cut(s) 742
Eco130I CCWWGG 1 cut(s) 125
Eco147I AGGCCT 1 cut(s) 786
Eco47I GGWCC 1 cut(s) 25
Eco57I CTGAAG 2 cut(s) 943, 1142
EcoRI GAATTC 2 cut(s) 688, 917
EcoRII CCWGG 3 cut(s) 166, 497, 517
EcoT14I CCWWGG 1 cut(s) 125
EcoT22I ATGCAT 1 cut(s) 1201
ErhI CCWWGG 1 cut(s) 125
FaeI CATG 8 cut(s) 10, 129, 182, 256, 455, 714, 1095, 1110
FalI AAGNNNNNCTT 2 cut(s) 741, 773
FaqI GGGAC 1 cut(s) 1009
FatI CATG 8 cut(s) 6, 125, 178, 252, 451, 710, 1091, 1106
FauNDI CATATG 1 cut(s) 544
FblI GTMKAC 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 281, 1059, 1185
FokI GGATG 2 cut(s) 386, 615
Fsp4HI GCNGC 3 cut(s) 281, 1059, 1185
FspBI CTAG 1 cut(s) 885
GluI GCNGC 3 cut(s) 281, 1059, 1185
GsuI CTGGAG 3 cut(s) 204, 801, 1158
HaeIII GGCC 3 cut(s) 522, 786, 1073
HgaI GACGC 1 cut(s) 1011
Hin1II CATG 8 cut(s) 10, 129, 182, 256, 455, 714, 1095, 1110
HincII GTYRAC 2 cut(s) 244, 474
HindII GTYRAC 2 cut(s) 244, 474
HinfI GANTC 5 cut(s) 267, 389, 508, 647, 1034
HphI GGTGA 2 cut(s) 889, 960
Hpy166II GTNNAC 5 cut(s) 199, 244, 474, 590, 1160
Hpy188I TCNGA 5 cut(s) 415, 528, 933, 943, 1122
Hpy188III TCNNGA 7 cut(s) 119, 368, 379, 818, 905, 1031, 1082
Hpy8I GTNNAC 5 cut(s) 199, 244, 474, 590, 1160
HpyAV CCTTC 9 cut(s) 37, 397, 430, 533, 797, 838, 1014, 1037, 1148
HpyCH4III ACNGT 4 cut(s) 203, 939, 972, 1164
HpyCH4IV ACGT 1 cut(s) 741
HpyCH4V TGCA 5 cut(s) 206, 280, 575, 867, 1199
HpyF3I CTNAG 3 cut(s) 35, 193, 930
HpySE526I ACGT 1 cut(s) 741
Hsp92II CATG 8 cut(s) 10, 129, 182, 256, 455, 714, 1095, 1110
Kzo9I GATC 6 cut(s) 115, 121, 595, 751, 820, 880
LmnI GCTCC 2 cut(s) 539, 797
Lsp1109I GCAGC 2 cut(s) 292, 1171
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 885
MaeII ACGT 1 cut(s) 741
MaeIII GTNAC 1 cut(s) 1015
MalI GATC 6 cut(s) 117, 123, 597, 753, 822, 882
MboI GATC 6 cut(s) 115, 121, 595, 751, 820, 880
MboII GAAGA 6 cut(s) 642, 743, 926, 949, 1135, 1240
MfeI CAATTG 1 cut(s) 273
MflI RGATCY 1 cut(s) 880
MhlI GDGCHC 1 cut(s) 582
MlsI TGGCCA 1 cut(s) 1073
MluCI AATT 8 cut(s) 70, 101, 273, 350, 683, 688, 917, 944
MluNI TGGCCA 1 cut(s) 1073
MlyI GAGTC 2 cut(s) 517, 1028
MmeI TCCRAC 1 cut(s) 775
Mox20I TGGCCA 1 cut(s) 1073
Mph1103I ATGCAT 1 cut(s) 1201
MscI TGGCCA 1 cut(s) 1073
MseI TTAA 3 cut(s) 69, 678, 875
MslI CAYNNNNRTG 3 cut(s) 397, 456, 875
Msp20I TGGCCA 1 cut(s) 1073
MspR9I CCNGG 3 cut(s) 168, 499, 519
MunI CAATTG 1 cut(s) 273
MvaI CCWGG 3 cut(s) 168, 499, 519
NcoI CCATGG 1 cut(s) 125
NdeI CATATG 1 cut(s) 544
NdeII GATC 6 cut(s) 115, 121, 595, 751, 820, 880
NlaIII CATG 8 cut(s) 10, 129, 182, 256, 455, 714, 1095, 1110
NlaIV GGNNCC 2 cut(s) 849, 882
NsiI ATGCAT 1 cut(s) 1201
NspI RCATGY 3 cut(s) 10, 256, 1095
PceI AGGCCT 1 cut(s) 786
PciI ACATGT 3 cut(s) 6, 252, 1091
PfeI GAWTC 3 cut(s) 267, 389, 647
Pfl23II CGTACG 1 cut(s) 972
PkrI GCNGC 3 cut(s) 282, 1060, 1186
PleI GAGTC 2 cut(s) 516, 1028
PpsI GAGTC 2 cut(s) 516, 1028
Ppu21I YACGTR 1 cut(s) 742
PscI ACATGT 3 cut(s) 6, 252, 1091
PshBI ATTAAT 1 cut(s) 69
Psp6I CCWGG 3 cut(s) 166, 497, 517
PspGI CCWGG 3 cut(s) 166, 497, 517
PspLI CGTACG 1 cut(s) 972
PspN4I GGNNCC 2 cut(s) 849, 882
PspPI GGNCC 1 cut(s) 25
PsuI RGATCY 1 cut(s) 880
RsaI GTAC 4 cut(s) 485, 744, 974, 1090
RsaNI GTAC 4 cut(s) 484, 743, 973, 1089
RseI CAYNNNNRTG 3 cut(s) 397, 456, 875
SaqAI TTAA 3 cut(s) 69, 678, 875
SatI GCNGC 3 cut(s) 281, 1059, 1185
Sau3AI GATC 6 cut(s) 115, 121, 595, 751, 820, 880
Sau96I GGNCC 1 cut(s) 25
SchI GAGTC 2 cut(s) 517, 1028
ScrFI CCNGG 3 cut(s) 168, 499, 519
SduI GDGCHC 1 cut(s) 582
SfaNI GCATC 1 cut(s) 216
SinI GGWCC 1 cut(s) 25
SmiMI CAYNNNNRTG 3 cut(s) 397, 456, 875
SmlI CTYRAG 2 cut(s) 987, 1036
SmoI CTYRAG 2 cut(s) 987, 1036
SnaBI TACGTA 1 cut(s) 742
Sse9I AATT 8 cut(s) 70, 101, 273, 350, 683, 688, 917, 944
SseBI AGGCCT 1 cut(s) 786
SsiI CCGC 1 cut(s) 1059
SspMI CTAG 1 cut(s) 885
StuI AGGCCT 1 cut(s) 786
StyD4I CCNGG 3 cut(s) 166, 497, 517
StyI CCWWGG 1 cut(s) 125
TaaI ACNGT 4 cut(s) 203, 939, 972, 1164
TaiI ACGT 1 cut(s) 744
TaqI TCGA 4 cut(s) 257, 336, 829, 1081
TasI AATT 8 cut(s) 70, 101, 273, 350, 683, 688, 917, 944
TatI WGTACW 2 cut(s) 483, 1088
TauI GCSGC 1 cut(s) 1061
TfiI GAWTC 3 cut(s) 267, 389, 647
Tru1I TTAA 3 cut(s) 69, 678, 875
Tru9I TTAA 3 cut(s) 69, 678, 875
TscAI CASTG 3 cut(s) 213, 228, 676
TseI GCWGC 2 cut(s) 280, 1184
TspDTI ATGAA 4 cut(s) 204, 432, 927, 1095
TspGWI ACGGA 2 cut(s) 459, 840
TspRI CASTG 3 cut(s) 213, 228, 676
VpaK11BI GGWCC 1 cut(s) 25
VspI ATTAAT 1 cut(s) 69
XapI RAATTY 4 cut(s) 350, 688, 917, 944
XceI RCATGY 3 cut(s) 10, 256, 1095
XmiI GTMKAC 1 cut(s) 198
XspI CTAG 1 cut(s) 885
Zsp2I ATGCAT 1 cut(s) 1201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.