RchiOBHm_Chr4g0437311

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0014498
Physical Location & Seq
Reverse (-)
60444477 .. 60450110
5634 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ40557

Sequence Viewer

Length: 2250 bp
ATGGCTCTTCTTTCCTTCATCCTCAACCAAAACTGCTCCCTCCGAAGGTCCCTCACTTTTGGCTTAATTCCCTACATATCCTCCTCCCTCTTCTCTACTCTTTGCAGAGACCACCCCTCCTCCCCTGCCCCACCGCTGGCCTCTCCGCCGGTCCCAAAGAAAGTACCTTTCACGGTCTCAGCTCAAGGCAGAACGTGGCAGGACCCCTACCGTTGGATGTCCAACACCGACGACCCTGATCTCTCCGACCACCTCAACCAGGAAAACTCATACGCCGATGCTTTCATGGCTGACACTCGCAGCCTCCAACGAACTCTGGTCTCTGAGATGACAAGTCGTGTGCCCACCAAGATTTCAACCCCTCCTGAGCTTTGGGGACCCTGGTTGTATTACCAATACATTCCAGAAGGGAAAGCATACCCGGTTCTTTGTAGAAGATTAGAAATCAAAAAGAAAGGTTGGATTAAGAGTTTTCTTTGCTATGCAAGAGGAGGATTTGGAAAGGAGGAAGTTTTGCTCGACTGGAATGAAATTGCCAAGAAATATGGAAAGCTTCTTTCTTTCTTTCTCATGGTTGTCTGCTCAAAACTAGGATATGATGATATGGAAAATATCACTGTGTTTACAGAAAGTGATTCCAGCTTCTGTGTGGACATAACAAGCACAAAAGATGGCAAGTTTATAACTGTCAATTCAAATTCAAGGACTTCATCTGAGGAAGGAATTTTACCAGTTCAACTTCTCTTCCCCTTTCAATGGAGCGTTTATTTGATAGATTCTGCCAACCCATTAGACAGATTGCAGAAAGTGTGGAAGCGTGTTCCTGGTGTACAGTATTTTCTGGAACATCATCATGGAATATTTTATGTTCTTACTAATGCTCCTTTGCGAGAAAGCAAGGAGTGGTCAGGTGAAGGTTATTACCTAGCTAGATGCTGTGTTGAAGATATACTGTCATCTAATTGGCAGAATATCATCATTCCAAGTGAAGACTTCAGCATTCAAGACATGGACATGTTTGATGGGCATCTGGTCCTTTTTCTCAACAAGAAGGATTACTCCGTCTTTTGTTCCATTGATTTGCCTATGGCTGTTCATTGTAAGCATCAAGTGAAGATTGAAGATCTTGATCCCTGGTTTTTCCCTCTGCCCTCAAATTCATGTAGCGTTGCTCCAGGTTCAAACCATGACTTCCAGAATTCAGCATACCGTGTGGTGCTTTCTTCTCCTGTGATGCCTGATGTAGTTGTTGACTATGACATGTCAACACGGAAATTCTCAGTTGTGCAGCAAGAAGAAGTGATGGGCTTTTGTGATTCAAGCTCACCAGCCAATGAGATAGATATTAACCAAACTCTTGTTAAACAAAATGAGAAGGATAAAAATGTCCAGATAAGTGAATCACAAAGATGGAAAGACTTTTCTGGTGAATACTGCTGTGAGAGGAGGGAAGTTATTTCCCATGATGGTGTTAAAGTTCCTTTGACCCTACTGTACTCTCATACGGCCTGGAAAAAGGGTCGGTCTCCTGGGCTTCTACACGGATATGGAGCATATGGGGAGGTTTTAGATAAAAGCTGGTGCACTGATCGGCTGAGTTTACTTGACCGGGGATGGGTGGTGGCATTTGCTGATGTGAGGGGTGGTGGTGGAGATTCTTCGTGGCATAAAGCTGGAAGTCAGTTTAATAAACTGAATTCGATATACGATTTTGTATCATGTGGCAACTACCTGATTAAAGAGGGTTATGTACATAGCGATCAGCTTGGTGCTATTGGACATAGTGCTGGAGGTCTACTTGTTGGAGCAACTATCAATAAGTACCCAGATCTATTTCGTGCTGCTATTTTGAAGGTTCCATTTCTTGACGTATGCAACACTCTAATGGATCCCAGCTTGCCCCTCACGATTCTGGATTATGAAGAATTTGGCAATCCTCAGATAAAGTCCCAATTTGAGCATATCTTGAGTTATTCACCTTATGATAATATCTCTCAAGGCAGTTGTTACCCTTCAATGCTTGTTACAGCGTCCTTTCATGACTCGAGGGTTGGGTTTTGGGAAGCTGCCAAGTGGGTGGCCAAAGTTCGAGATAGCACATGTCCTCGTTGTTCTTGTTCTGTTATTCTGAAGACAAATATGACCGGAGGACATTTTGGCGAAGGTGGACGCTATGCTCAAAATGAGGAAACTGCGTATGATTATGCTTTCCTTTTTAAAGCTATGGGGATGCTGAAGAATGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

749

Amino Acids

84.65

Weight (kDa)

5.81

Isoelectric Point (pI)

48.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 49 - 181 1.5e-21 Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9_N PF02897 192 - 431 4.7e-10 Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9 PF00326 531 - 743 6.9e-45 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69020 AT1G69020
fragaria_vesca FvH4_4g29260
malus_domestica MD13G1056200.v1.1
prunus_persica Prupe.1G293900_v2.0.a1
pyrus_communis pycom13g05020
rosa_chinensis RchiOBHm_Chr4g0437311
rosa_laevigata RLG00000006447
rosa_multiflora Rmu_co8522681.1_g000001 Rmu_sc0004339.1_g000034
rosa_roxburghii Rroxscaffold_5G00378460
rosa_rugosa Rorug04G0299500
rosa_samantha Rh4AG354500 Rh4BG364300 Rh4CG378600 Rh4DG358200
rosa_wichuraiana Rw4G031080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 683
AccI GTMKAC 1 cut(s) 1795
AciI CCGC 2 cut(s) 134, 146
AclWI GGATC 3 cut(s) 1124, 1883, 1896
AcoI YGGCCR 1 cut(s) 2079
AcsI RAATTY 7 cut(s) 697, 723, 1156, 1198, 1274, 1696, 1925
AcuI CTGAAG 2 cut(s) 979, 2150
AfaI GTAC 5 cut(s) 165, 831, 1496, 1752, 1823
AfiI CCNNNNNNNGG 6 cut(s) 45, 136, 213, 259, 756, 1615
AflIII ACRYGT 3 cut(s) 1014, 1260, 2099
AjnI CCWGG 7 cut(s) 258, 380, 823, 1133, 1174, 1508, 1528
Alw21I GWGCWC 1 cut(s) 1586
Alw26I GTCTC 4 cut(s) 102, 181, 325, 1530
Alw44I GTGCAC 1 cut(s) 1582
AlwI GGATC 3 cut(s) 1124, 1883, 1896
AlwNI CAGNNNCTG 1 cut(s) 645
Ama87I CYCGRG 1 cut(s) 2044
AoxI GGCC 3 cut(s) 138, 1506, 2079
ApaLI GTGCAC 1 cut(s) 1582
ApeKI GCWGC 4 cut(s) 300, 1288, 1841, 2066
ApoI RAATTY 7 cut(s) 697, 723, 1156, 1198, 1274, 1696, 1925
AspS9I GGNCC 5 cut(s) 48, 151, 202, 377, 1033
AsuC2I CCSGG 2 cut(s) 422, 1610
AsuHPI GGTGA 4 cut(s) 923, 1317, 1439, 1968
AvaI CYCGRG 1 cut(s) 2044
AvaII GGWCC 5 cut(s) 48, 151, 202, 377, 1033
BaeGI GKGCMC 2 cut(s) 345, 1586
BalI TGGCCA 1 cut(s) 2081
BamHI GGATCC 1 cut(s) 1888
BarI GAAGNNNNNNTAC 2 cut(s) 711, 743
BbsI GAAGAC 2 cut(s) 996, 2138
Bbv12I GWGCWC 1 cut(s) 1586
BbvI GCAGC 4 cut(s) 312, 1300, 1828, 2053
BccI CCATC 6 cut(s) 665, 1016, 1297, 1404, 1460, 1608
BceAI ACGGC 1 cut(s) 1521
BciT130I CCWGG 7 cut(s) 260, 382, 825, 1135, 1176, 1510, 1530
BcnI CCSGG 2 cut(s) 422, 1610
BcoDI GTCTC 4 cut(s) 102, 181, 325, 1530
BfaI CTAG 3 cut(s) 590, 926, 930
BglII AGATCT 2 cut(s) 1123, 1828
BisI GCNGC 4 cut(s) 301, 1289, 1842, 2067
BlsI GCNGC 4 cut(s) 302, 1290, 1843, 2068
Bme1390I CCNGG 9 cut(s) 260, 382, 422, 825, 1135, 1176, 1510, 1530, 1610
Bme18I GGWCC 5 cut(s) 48, 151, 202, 377, 1033
BmeT110I CYCGRG 1 cut(s) 2044
BmgT120I GGNCC 5 cut(s) 48, 151, 202, 377, 1033
BmiI GGNNCC 7 cut(s) 50, 153, 204, 378, 379, 1857, 1890
BmrFI CCNGG 9 cut(s) 260, 382, 422, 825, 1135, 1176, 1510, 1530, 1610
BmsI GCATC 6 cut(s) 268, 923, 1036, 1114, 1224, 2220
BpiI GAAGAC 2 cut(s) 996, 2138
BpmI CTGGAG 2 cut(s) 1158, 1809
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 3 cut(s) 168, 1980, 1987
BpuMI CCSGG 2 cut(s) 422, 1610
BsaBI GATNNNNATC 2 cut(s) 1026, 1128
BsaI GGTCTC 4 cut(s) 102, 181, 325, 1530
BsaJI CCNNGG 4 cut(s) 380, 1133, 1529, 1609
BsaWI WCCGGW 1 cut(s) 2144
BsaXI ACNNNNNCTCC 8 cut(s) 65, 95, 101, 104, 131, 134, 865, 895
Bsc4I CCNNNNNNNGG 6 cut(s) 45, 136, 213, 259, 756, 1615
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 2 cut(s) 527, 731
Bse8I GATNNNNATC 2 cut(s) 1026, 1128
BseBI CCWGG 7 cut(s) 260, 382, 825, 1135, 1176, 1510, 1530
BseDI CCNNGG 4 cut(s) 380, 1133, 1529, 1609
BseGI GGATG 4 cut(s) 18, 222, 1619, 2235
BseJI GATNNNNATC 2 cut(s) 1026, 1128
BseLI CCNNNNNNNGG 6 cut(s) 45, 136, 213, 259, 756, 1615
BseMII CTCAG 7 cut(s) 192, 315, 357, 705, 1293, 1586, 1952
BseNI ACTGG 2 cut(s) 527, 731
BseRI GAGGAG 4 cut(s) 73, 109, 504, 1459
BseSI GKGCMC 2 cut(s) 345, 1586
BseXI GCAGC 4 cut(s) 312, 1300, 1828, 2053
BseYI CCCAGC 1 cut(s) 1892
BsgI GTGCAG 1 cut(s) 1307
BshFI GGCC 3 cut(s) 140, 1508, 2081
BsiHKAI GWGCWC 1 cut(s) 1586
BsiHKCI CYCGRG 1 cut(s) 2044
BsiSI CCGG 4 cut(s) 149, 422, 1609, 2145
BslFI GGGAC 4 cut(s) 34, 137, 390, 1933
BslI CCNNNNNNNGG 6 cut(s) 45, 136, 213, 259, 756, 1615
BsmAI GTCTC 4 cut(s) 102, 181, 325, 1530
BsmFI GGGAC 4 cut(s) 34, 137, 390, 1933
BsmI GAATGC 1 cut(s) 999
BsnI GGCC 3 cut(s) 140, 1508, 2081
Bso31I GGTCTC 4 cut(s) 102, 181, 325, 1530
BsoBI CYCGRG 1 cut(s) 2044
Bsp1286I GDGCHC 2 cut(s) 345, 1586
Bsp1407I TGTACA 2 cut(s) 829, 1750
Bsp143I GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
BspACI CCGC 2 cut(s) 134, 146
BspANI GGCC 3 cut(s) 140, 1508, 2081
BspCNI CTCAG 7 cut(s) 191, 316, 358, 706, 1292, 1587, 1951
BspHI TCATGA 1 cut(s) 2038
BspLI GGNNCC 7 cut(s) 50, 153, 204, 378, 379, 1857, 1890
BspPI GGATC 3 cut(s) 1124, 1883, 1896
BspQI GCTCTTC 1 cut(s) 12
BspTNI GGTCTC 4 cut(s) 102, 181, 325, 1530
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 2 cut(s) 829, 1750
BsrI ACTGG 2 cut(s) 527, 731
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 380, 1133, 1529, 1609
BssMI GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
Bst2UI CCWGG 7 cut(s) 260, 382, 825, 1135, 1176, 1510, 1530
Bst4CI ACNGT 8 cut(s) 175, 212, 619, 688, 834, 954, 1211, 1494
Bst6I CTCTTC 3 cut(s) 12, 95, 749
BstAUI TGTACA 2 cut(s) 829, 1750
BstC8I GCNNGC 2 cut(s) 138, 1898
BstDEI CTNAG 7 cut(s) 178, 324, 366, 714, 1279, 1595, 1938
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 4 cut(s) 18, 222, 1619, 2235
BstKTI GATC 7 cut(s) 241, 1126, 1132, 1591, 1762, 1831, 1891
BstMAI GTCTC 4 cut(s) 102, 181, 325, 1530
BstMBI GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 7 cut(s) 260, 382, 825, 1135, 1176, 1510, 1530
BstNSI RCATGY 3 cut(s) 1018, 1264, 2103
BstSCI CCNGG 9 cut(s) 258, 380, 420, 823, 1133, 1174, 1508, 1528, 1608
BstSLI GKGCMC 2 cut(s) 345, 1586
BstV1I GCAGC 4 cut(s) 312, 1300, 1828, 2053
BstV2I GAAGAC 2 cut(s) 996, 2138
BstX2I RGATCY 3 cut(s) 1123, 1828, 1888
BstXI CCANNNNNNTGG 1 cut(s) 2077
BstYI RGATCY 3 cut(s) 1123, 1828, 1888
BsuRI GGCC 3 cut(s) 140, 1508, 2081
BtsCI GGATG 4 cut(s) 18, 222, 1619, 2235
BtsIMutI CAGTG 2 cut(s) 615, 1584
Cac8I GCNNGC 2 cut(s) 138, 1898
CaiI CAGNNNCTG 1 cut(s) 645
CciI TCATGA 1 cut(s) 2038
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 5 cut(s) 48, 151, 202, 377, 1033
CseI GACGC 2 cut(s) 2019, 2178
Csp6I GTAC 5 cut(s) 164, 830, 1495, 1751, 1822
CviQI GTAC 5 cut(s) 164, 830, 1495, 1751, 1822
DdeI CTNAG 7 cut(s) 178, 324, 366, 714, 1279, 1595, 1938
DpnI GATC 7 cut(s) 240, 1125, 1131, 1590, 1761, 1830, 1890
DpnII GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
DraI TTTAAA 1 cut(s) 2218
EaeI YGGCCR 1 cut(s) 2079
Eam1104I CTCTTC 3 cut(s) 12, 95, 749
EarI CTCTTC 3 cut(s) 12, 95, 749
EciI GGCGGA 1 cut(s) 135
Eco31I GGTCTC 4 cut(s) 102, 181, 325, 1530
Eco47I GGWCC 5 cut(s) 48, 151, 202, 377, 1033
Eco57I CTGAAG 2 cut(s) 979, 2150
Eco88I CYCGRG 1 cut(s) 2044
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 3 cut(s) 48, 202, 377
EcoRI GAATTC 2 cut(s) 1198, 1696
EcoRII CCWGG 7 cut(s) 258, 380, 823, 1133, 1174, 1508, 1528
FaqI GGGAC 4 cut(s) 34, 137, 390, 1933
FauNDI CATATG 1 cut(s) 1555
FblI GTMKAC 1 cut(s) 1795
Fnu4HI GCNGC 4 cut(s) 301, 1289, 1842, 2067
FokI GGATG 4 cut(s) 5, 229, 1626, 2242
Fsp4HI GCNGC 4 cut(s) 301, 1289, 1842, 2067
FspBI CTAG 3 cut(s) 590, 926, 930
GluI GCNGC 4 cut(s) 301, 1289, 1842, 2067
GsaI CCCAGC 1 cut(s) 1896
GsuI CTGGAG 2 cut(s) 1158, 1809
HaeIII GGCC 3 cut(s) 140, 1508, 2081
HapII CCGG 4 cut(s) 149, 422, 1609, 2145
HgaI GACGC 2 cut(s) 2019, 2178
HincII GTYRAC 2 cut(s) 1252, 1266
HindII GTYRAC 2 cut(s) 1252, 1266
HindIII AAGCTT 1 cut(s) 551
HinfI GANTC 7 cut(s) 635, 776, 1316, 1400, 1655, 1909, 2042
HpaII CCGG 4 cut(s) 149, 422, 1609, 2145
HphI GGTGA 4 cut(s) 923, 1317, 1439, 1968
Hpy166II GTNNAC 9 cut(s) 624, 652, 830, 1252, 1266, 1584, 1601, 1796, 2168
Hpy188I TCNGA 6 cut(s) 44, 247, 325, 715, 1941, 2130
Hpy8I GTNNAC 9 cut(s) 624, 652, 830, 1252, 1266, 1584, 1601, 1796, 2168
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 8 cut(s) 175, 212, 619, 688, 834, 954, 1211, 1494
HpyCH4IV ACGT 2 cut(s) 194, 1869
HpyCH4V TGCA 6 cut(s) 105, 485, 802, 1288, 1584, 1875
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 7 cut(s) 178, 324, 366, 714, 1279, 1595, 1938
HpySE526I ACGT 2 cut(s) 194, 1869
KflI GGGWCCC 1 cut(s) 377
Kzo9I GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 6 cut(s) 41, 759, 886, 1177, 1550, 1805
Lsp1109I GCAGC 4 cut(s) 312, 1300, 1828, 2053
LweI GCATC 6 cut(s) 268, 923, 1036, 1114, 1224, 2220
MaeI CTAG 3 cut(s) 590, 926, 930
MaeII ACGT 2 cut(s) 194, 1869
MaeIII GTNAC 2 cut(s) 2006, 2023
MalI GATC 7 cut(s) 240, 1125, 1131, 1590, 1761, 1830, 1890
MboI GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
MflI RGATCY 3 cut(s) 1123, 1828, 1888
MhlI GDGCHC 2 cut(s) 345, 1586
MlsI TGGCCA 1 cut(s) 2081
MluNI TGGCCA 1 cut(s) 2081
MlyI GAGTC 1 cut(s) 2036
MmeI TCCRAC 6 cut(s) 194, 246, 270, 331, 440, 1783
Mox20I TGGCCA 1 cut(s) 2081
MscI TGGCCA 1 cut(s) 2081
MseI TTAA 8 cut(s) 65, 465, 1347, 1362, 1473, 1686, 1737, 2217
MslI CAYNNNNRTG 6 cut(s) 852, 1013, 1408, 1467, 1545, 1883
Msp20I TGGCCA 1 cut(s) 2081
MspA1I CMGCKG 1 cut(s) 136
MspI CCGG 4 cut(s) 149, 422, 1609, 2145
MspR9I CCNGG 9 cut(s) 260, 382, 422, 825, 1135, 1176, 1510, 1530, 1610
Mva1269I GAATGC 1 cut(s) 999
MvaI CCWGG 7 cut(s) 260, 382, 825, 1135, 1176, 1510, 1530
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 2 cut(s) 422, 1610
NdeI CATATG 1 cut(s) 1555
NdeII GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
NlaIV GGNNCC 7 cut(s) 50, 153, 204, 378, 379, 1857, 1890
NspI RCATGY 3 cut(s) 1018, 1264, 2103
PaeR7I CTCGAG 1 cut(s) 2044
PagI TCATGA 1 cut(s) 2038
PciI ACATGT 3 cut(s) 1014, 1260, 2099
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 999
PfeI GAWTC 6 cut(s) 635, 776, 1316, 1400, 1655, 1909
PkrI GCNGC 4 cut(s) 302, 1290, 1843, 2068
PleI GAGTC 1 cut(s) 2036
PpsI GAGTC 1 cut(s) 2036
PpuMI RGGWCCY 3 cut(s) 48, 202, 377
PscI ACATGT 3 cut(s) 1014, 1260, 2099
PsiI TTATAA 1 cut(s) 683
Psp5II RGGWCCY 3 cut(s) 48, 202, 377
Psp6I CCWGG 7 cut(s) 258, 380, 823, 1133, 1174, 1508, 1528
PspFI CCCAGC 1 cut(s) 1892
PspGI CCWGG 7 cut(s) 258, 380, 823, 1133, 1174, 1508, 1528
PspN4I GGNNCC 7 cut(s) 50, 153, 204, 378, 379, 1857, 1890
PspPI GGNCC 5 cut(s) 48, 151, 202, 377, 1033
PspPPI RGGWCCY 3 cut(s) 48, 202, 377
PspXI VCTCGAGB 1 cut(s) 2044
PstNI CAGNNNCTG 1 cut(s) 645
PsuI RGATCY 3 cut(s) 1123, 1828, 1888
RsaI GTAC 5 cut(s) 165, 831, 1496, 1752, 1823
RsaNI GTAC 5 cut(s) 164, 830, 1495, 1751, 1822
RseI CAYNNNNRTG 6 cut(s) 852, 1013, 1408, 1467, 1545, 1883
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 8 cut(s) 65, 465, 1347, 1362, 1473, 1686, 1737, 2217
SatI GCNGC 4 cut(s) 301, 1289, 1842, 2067
Sau3AI GATC 7 cut(s) 238, 1123, 1129, 1588, 1759, 1828, 1888
Sau96I GGNCC 5 cut(s) 48, 151, 202, 377, 1033
SchI GAGTC 1 cut(s) 2036
ScrFI CCNGG 9 cut(s) 260, 382, 422, 825, 1135, 1176, 1510, 1530, 1610
SduI GDGCHC 2 cut(s) 345, 1586
SfaNI GCATC 6 cut(s) 268, 923, 1036, 1114, 1224, 2220
Sfr274I CTCGAG 1 cut(s) 2044
SinI GGWCC 5 cut(s) 48, 151, 202, 377, 1033
SlaI CTCGAG 1 cut(s) 2044
SmiMI CAYNNNNRTG 6 cut(s) 852, 1013, 1408, 1467, 1545, 1883
SmlI CTYRAG 4 cut(s) 183, 1966, 1995, 2044
SmoI CTYRAG 4 cut(s) 183, 1966, 1995, 2044
SsiI CCGC 2 cut(s) 134, 146
SspI AATATT 1 cut(s) 861
SspMI CTAG 3 cut(s) 590, 926, 930
StyD4I CCNGG 9 cut(s) 258, 380, 420, 823, 1133, 1174, 1508, 1528, 1608
TaaI ACNGT 8 cut(s) 175, 212, 619, 688, 834, 954, 1211, 1494
TaiI ACGT 2 cut(s) 197, 1872
TaqI TCGA 4 cut(s) 519, 1700, 2045, 2089
TaqII GACCGA 1 cut(s) 1512
TatI WGTACW 3 cut(s) 829, 1494, 1750
TfiI GAWTC 6 cut(s) 635, 776, 1316, 1400, 1655, 1909
Tru1I TTAA 8 cut(s) 65, 465, 1347, 1362, 1473, 1686, 1737, 2217
Tru9I TTAA 8 cut(s) 65, 465, 1347, 1362, 1473, 1686, 1737, 2217
TscAI CASTG 2 cut(s) 622, 1591
TseI GCWGC 4 cut(s) 300, 1288, 1841, 2066
TspDTI ATGAA 8 cut(s) 7, 274, 543, 699, 1085, 1149, 1935, 2027
TspGWI ACGGA 3 cut(s) 1051, 1285, 1557
TspRI CASTG 2 cut(s) 622, 1591
VneI GTGCAC 1 cut(s) 1582
VpaK11BI GGWCC 5 cut(s) 48, 151, 202, 377, 1033
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 7 cut(s) 697, 723, 1156, 1198, 1274, 1696, 1925
XceI RCATGY 3 cut(s) 1018, 1264, 2103
XcmI CCANNNNNNNNNTGG 1 cut(s) 646
XhoI CTCGAG 1 cut(s) 2044
XmiI GTMKAC 1 cut(s) 1795
XspI CTAG 3 cut(s) 590, 926, 930
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.