Rw4G031080

Prolyl oligopeptidase, N-terminal beta-propeller domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0014498
Physical Location & Seq
Reverse (-)
57946399 .. 57951270
4872 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G031080.1

Sequence Viewer

Length: 2442 bp
ATGGCTCTTCTTTCCTTCATCCTCAACCAAAACTGCTCCCTCCGAAGGTCCCTCACTTTTGGCTTAATTCCCTACATATCCTCCTCCCTCTTCTCTACTCTTTGCAGAGACCACCCCTCCTCCCCTGCCCCACCGCTAGCCTCTCCGCCGGTCCCAAAGAAAGTACCTTTCACGGTCTCAGCTCACGGCAGAACGTGGCAGGACCCCTACCGTTGGATGTCCAACACCGACGACCCTGATCTCTCCGACCACCTCAACCAGGAAAACTCATACGCCGATGCTTTCATGGCTGACACTCGCAGCCTCCAACGAACTCTGGTCTCTGAGATGACAAGTCGGGTGCCCACCAAGATTTCAACCCCTCCTGAGCTTTGGGGACCCTGGTTGTATTACCAATACATTCCAGAAGGGAAAGAATACCCGGTTCTTTGTAGAAGATTAGAAATCAAAAAGATGGGTTGGATTAAGAGTTTTCTTTGCTATGCAAGAGGAGGATTGGAAAGGAGGAAGTTTTGCTCGACTGGAATGAAATTGCCAAGAAATATGGAAAGCTTCTTTCTTTCTTTCTCATGTTATGTGCATGTTGGTACGTGTCGAATTTCACCAGACCACCATTTCCTAGCCTACACACTCGATATCAAGGGTGATGAGCAGTTCATTCTTCAAATTAAGGACCTAAGACGTGGATGTATCATTCCCAAAGTACATGTTAATGGTGTTGTTAGCTTGGCATGGGGTCAGGATAGCAGTACTCTGTTTTATACACTATCAGATGAGAATCAACGACCTTACAGGGTTGTCTGCTCAAAACTAGGATCTGATGATATGGAAAATATCACTGTGTTTACAGAAAGTGATTCCAGCTTCTGTGTGGACATAACAAGCACAAAAGATGGCAAGTTTATAACTGTCAATTCAAATTCAAGGACTTCATCTGAGGAAGGAATTTTACTAGTTTATTTGATAGATTCTGCCAACCCATTAGACAGATTGCAGAAAGTGTGGAAGCGTGTTCCTGGTGTACAGTATTTTCTGGAACATCATCATGGAATATTTTATGTTCTTACTAATGCTCCTTTGCGAGAAAGCAAGGAGTGGTCAGGTGAAGGTTATTACCTAGCTAGATGCCATGTTGAAGATATACTGTCATCTAATTGGCAGAATATCATCATTCCAAGTGAAGACTTCAGCATTCAAGACATGGACATGTTTGATGGGCATCTGGTCCTTTTTCTCAACAAGAAGGATTACTCCGTCTTTTGTTCCATTGATTTGCCTATGGCTGTTCATTGTAAGCATCAAGTGAACATTGAAGATCTTGATCCCTGGTTTTTCCCTCTGCCCTCAAATTCATGTAGCGTTGCTCCAGGTTCAAACCATGACTTCCAGAATTCAGCATACCGTGTGGTGCTTTCTTCTCCTGTGATGCCTGATGTAGTTGTTGACTATGGCATGTCAACACGGAGATTCTCAGTTGTGCAGCAAGAAGAAGTGATGGGCTTTTGTGATTCAAGCTCACCAGCCAATAAGATAGATATGAACCAAACTCTTGTTAAACAAAATGAGAAGGATAAAAATGTCCAGATAAGTGAATCACAAAGATGGAAAGACTTTTCTGGTGAATACTGCTGTGAGAGGAGGGAAGTTATTTCCCATGATGGTGTTAAAGTTCCTTTGACCTTACTGTACTCTCATACGGCCTGGAAAAAGGGTCGGTCTCCTGGGCTTCTACACGGATATGGAGCATATGGGGAGGTTTTAGATAAAAGCTGGTGCACTGATCGGCTGAGTTTACTTGACCGGGGATGGGTGGTGGCATTTGCTGATGTGAGGGGTAGTGGTGGAGATTCTTCGTGGCATAAAGCTGGAAGTCAGTTTAATAAACTGAATTCGATATACGATTTTGTATCATGTGGCAACTACCTGATTAAAGAGGGTTATGTACATAGCGATCAGCTTGGTGCTATTGGACATAGTGCTGGAGGTCTACTTGTTGGAGCAACTATCAATAAGTACCCAGATCTATTTCGTGCTGCTATTTTGAAGGTTCCATTTCTTGACGTATGCAACACTCTAATGGATCCCAGCTTGCCCCTCACGATTCTGGATTATGAAGAATTTGGCAATCCTCAGATAAAGTCCCAATTTGAGCATATCTTGAGTTATTCACCTTATGATAATATCTCTCAAGGCAGTTGTTACCCTTCAATGCTTGTTACAGCGTCCTTTCATGACTCGAGGGTCGGGTTTTGGGAAGCTGCCAAGTGGGTGGCCAAAGTTCGAGATAGCACATGTCCTCGTTGTTCTTGTTCTGTTATTCTGAAGACAAATATGACCGGAGGACATTTTGGCGAAGGTGGACGCTATGCTCAAAGTGAGGAAACTGCGTATGATTATGCTTTCCTTTTTAAAGCTATGGGGATGCTGAAGAATGAAAAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Peptidase_S9 Peptidase_S9_N

Protein Analysis

813

Amino Acids

91.93

Weight (kDa)

6.19

Isoelectric Point (pI)

49.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_S9_N PF02897 49 - 495 5.1e-48 Prolyl oligopeptidase, N-terminal beta-propeller domain
Peptidase_S9 PF00326 595 - 807 6.1e-45 Prolyl oligopeptidase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0014498)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G69020 AT1G69020
fragaria_vesca FvH4_4g29260
malus_domestica MD13G1056200.v1.1
prunus_persica Prupe.1G293900_v2.0.a1
pyrus_communis pycom13g05020
rosa_chinensis RchiOBHm_Chr4g0437311
rosa_laevigata RLG00000006447
rosa_multiflora Rmu_co8522681.1_g000001 Rmu_sc0004339.1_g000034
rosa_roxburghii Rroxscaffold_5G00378460
rosa_rugosa Rorug04G0299500
rosa_samantha Rh4AG354500 Rh4BG364300 Rh4CG378600 Rh4DG358200
rosa_wichuraiana Rw4G031080

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 905
AasI GACNNNNNNGTC 1 cut(s) 2240
AccB1I GGYRCC 1 cut(s) 340
AccI GTMKAC 1 cut(s) 1987
AciI CCGC 2 cut(s) 134, 146
AclWI GGATC 4 cut(s) 823, 1316, 2075, 2088
AcoI YGGCCR 1 cut(s) 2271
AcsI RAATTY 7 cut(s) 597, 919, 945, 1348, 1390, 1888, 2117
AcuI CTGAAG 2 cut(s) 1171, 2342
AfaI GTAC 8 cut(s) 165, 589, 705, 751, 1023, 1688, 1944, 2015
AfiI CCNNNNNNNGG 4 cut(s) 45, 213, 259, 1807
AflIII ACRYGT 4 cut(s) 590, 706, 1206, 2291
AhlI ACTAGT 1 cut(s) 952
AjiI CACGTC 1 cut(s) 683
AjnI CCWGG 7 cut(s) 258, 380, 1015, 1325, 1366, 1700, 1720
Alw21I GWGCWC 1 cut(s) 1778
Alw26I GTCTC 4 cut(s) 102, 181, 325, 1722
Alw44I GTGCAC 1 cut(s) 1774
AlwI GGATC 4 cut(s) 823, 1316, 2075, 2088
AlwNI CAGNNNCTG 1 cut(s) 867
Ama87I CYCGRG 1 cut(s) 2236
AoxI GGCC 2 cut(s) 1698, 2271
ApaLI GTGCAC 1 cut(s) 1774
ApeKI GCWGC 4 cut(s) 300, 1480, 2033, 2258
ApoI RAATTY 7 cut(s) 597, 919, 945, 1348, 1390, 1888, 2117
AspS9I GGNCC 6 cut(s) 48, 151, 202, 377, 673, 1225
AsuC2I CCSGG 2 cut(s) 422, 1802
AsuHPI GGTGA 6 cut(s) 594, 656, 1115, 1509, 1631, 2160
AsuNHI GCTAGC 1 cut(s) 136
AvaI CYCGRG 1 cut(s) 2236
AvaII GGWCC 6 cut(s) 48, 151, 202, 377, 673, 1225
BaeGI GKGCMC 2 cut(s) 345, 1778
BalI TGGCCA 1 cut(s) 2273
BamHI GGATCC 1 cut(s) 2080
BanI GGYRCC 1 cut(s) 340
BarI GAAGNNNNNNTAC 2 cut(s) 933, 965
BbsI GAAGAC 2 cut(s) 1188, 2330
Bbv12I GWGCWC 1 cut(s) 1778
BbvI GCAGC 4 cut(s) 312, 1492, 2020, 2245
BccI CCATC 7 cut(s) 448, 887, 1208, 1489, 1596, 1652, 1800
BceAI ACGGC 2 cut(s) 202, 1713
BciT130I CCWGG 7 cut(s) 260, 382, 1017, 1327, 1368, 1702, 1722
BcnI CCSGG 2 cut(s) 422, 1802
BcoDI GTCTC 4 cut(s) 102, 181, 325, 1722
BcuI ACTAGT 1 cut(s) 952
BfaI CTAG 6 cut(s) 137, 620, 812, 953, 1118, 1122
BglII AGATCT 2 cut(s) 1315, 2020
BisI GCNGC 4 cut(s) 301, 1481, 2034, 2259
BlsI GCNGC 4 cut(s) 302, 1482, 2035, 2260
BmcAI AGTACT 1 cut(s) 751
Bme1390I CCNGG 9 cut(s) 260, 382, 422, 1017, 1327, 1368, 1702, 1722, 1802
Bme18I GGWCC 6 cut(s) 48, 151, 202, 377, 673, 1225
BmeT110I CYCGRG 1 cut(s) 2236
BmgBI CACGTC 1 cut(s) 683
BmgT120I GGNCC 6 cut(s) 48, 151, 202, 377, 673, 1225
BmiI GGNNCC 8 cut(s) 50, 153, 204, 342, 378, 379, 2049, 2082
BmrFI CCNGG 9 cut(s) 260, 382, 422, 1017, 1327, 1368, 1702, 1722, 1802
BmsI GCATC 6 cut(s) 268, 1115, 1228, 1306, 1416, 2412
BmtI GCTAGC 1 cut(s) 140
BpiI GAAGAC 2 cut(s) 1188, 2330
BpmI CTGGAG 2 cut(s) 1350, 2001
Bpu10I CCTNAGC 1 cut(s) 366
BpuEI CTTGAG 2 cut(s) 2172, 2179
BpuMI CCSGG 2 cut(s) 422, 1802
BsaAI YACGTR 1 cut(s) 591
BsaBI GATNNNNATC 3 cut(s) 777, 1218, 1320
BsaI GGTCTC 4 cut(s) 102, 181, 325, 1722
BsaJI CCNNGG 4 cut(s) 380, 1325, 1721, 1801
BsaWI WCCGGW 1 cut(s) 2336
BsaXI ACNNNNNCTCC 8 cut(s) 65, 95, 101, 104, 131, 134, 1057, 1087
Bsc4I CCNNNNNNNGG 4 cut(s) 45, 213, 259, 1807
Bse118I RCCGGY 1 cut(s) 148
Bse1I ACTGG 1 cut(s) 526
Bse8I GATNNNNATC 3 cut(s) 777, 1218, 1320
BseBI CCWGG 7 cut(s) 260, 382, 1017, 1327, 1368, 1702, 1722
BseDI CCNNGG 4 cut(s) 380, 1325, 1721, 1801
BseGI GGATG 5 cut(s) 18, 222, 692, 1811, 2427
BseJI GATNNNNATC 3 cut(s) 777, 1218, 1320
BseLI CCNNNNNNNGG 4 cut(s) 45, 213, 259, 1807
BseMII CTCAG 7 cut(s) 192, 315, 357, 927, 1485, 1778, 2144
BseNI ACTGG 1 cut(s) 526
BseRI GAGGAG 4 cut(s) 73, 109, 504, 1651
BseSI GKGCMC 2 cut(s) 345, 1778
BseXI GCAGC 4 cut(s) 312, 1492, 2020, 2245
BseYI CCCAGC 1 cut(s) 2084
BsgI GTGCAG 1 cut(s) 1499
BshFI GGCC 2 cut(s) 1700, 2273
BshNI GGYRCC 1 cut(s) 340
BsiHKAI GWGCWC 1 cut(s) 1778
BsiHKCI CYCGRG 1 cut(s) 2236
BsiSI CCGG 4 cut(s) 149, 422, 1801, 2337
BslFI GGGAC 4 cut(s) 34, 137, 390, 2125
BslI CCNNNNNNNGG 4 cut(s) 45, 213, 259, 1807
BsmAI GTCTC 4 cut(s) 102, 181, 325, 1722
BsmFI GGGAC 4 cut(s) 34, 137, 390, 2125
BsmI GAATGC 1 cut(s) 1191
BsnI GGCC 2 cut(s) 1700, 2273
Bso31I GGTCTC 4 cut(s) 102, 181, 325, 1722
BsoBI CYCGRG 1 cut(s) 2236
Bsp1286I GDGCHC 2 cut(s) 345, 1778
Bsp1407I TGTACA 2 cut(s) 1021, 1942
Bsp143I GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
BspACI CCGC 2 cut(s) 134, 146
BspANI GGCC 2 cut(s) 1700, 2273
BspCNI CTCAG 7 cut(s) 191, 316, 358, 928, 1484, 1779, 2143
BspHI TCATGA 1 cut(s) 2230
BspLI GGNNCC 8 cut(s) 50, 153, 204, 342, 378, 379, 2049, 2082
BspOI GCTAGC 1 cut(s) 140
BspPI GGATC 4 cut(s) 823, 1316, 2075, 2088
BspQI GCTCTTC 1 cut(s) 12
BspT107I GGYRCC 1 cut(s) 340
BspTNI GGTCTC 4 cut(s) 102, 181, 325, 1722
BsrFI RCCGGY 1 cut(s) 148
BsrGI TGTACA 2 cut(s) 1021, 1942
BsrI ACTGG 1 cut(s) 526
BssAI RCCGGY 1 cut(s) 148
BssECI CCNNGG 4 cut(s) 380, 1325, 1721, 1801
BssMI GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
Bst2UI CCWGG 7 cut(s) 260, 382, 1017, 1327, 1368, 1702, 1722
Bst4CI ACNGT 8 cut(s) 175, 212, 841, 910, 1026, 1146, 1403, 1686
Bst6I CTCTTC 2 cut(s) 12, 95
BstAUI TGTACA 2 cut(s) 1021, 1942
BstBAI YACGTR 1 cut(s) 591
BstC8I GCNNGC 2 cut(s) 138, 2090
BstDEI CTNAG 8 cut(s) 178, 324, 366, 677, 936, 1471, 1787, 2130
BstENI CCTNNNNNAGG 1 cut(s) 257
BstF5I GGATG 5 cut(s) 18, 222, 692, 1811, 2427
BstKTI GATC 8 cut(s) 241, 818, 1318, 1324, 1783, 1954, 2023, 2083
BstMAI GTCTC 4 cut(s) 102, 181, 325, 1722
BstMBI GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
BstMWI GCNNNNNNNGC 1 cut(s) 287
BstNI CCWGG 7 cut(s) 260, 382, 1017, 1327, 1368, 1702, 1722
BstNSI RCATGY 5 cut(s) 584, 710, 1210, 1456, 2295
BstSCI CCNGG 9 cut(s) 258, 380, 420, 1015, 1325, 1366, 1700, 1720, 1800
BstSLI GKGCMC 2 cut(s) 345, 1778
BstV1I GCAGC 4 cut(s) 312, 1492, 2020, 2245
BstV2I GAAGAC 2 cut(s) 1188, 2330
BstX2I RGATCY 4 cut(s) 815, 1315, 2020, 2080
BstXI CCANNNNNNTGG 1 cut(s) 2269
BstYI RGATCY 4 cut(s) 815, 1315, 2020, 2080
BsuRI GGCC 2 cut(s) 1700, 2273
BtrI CACGTC 1 cut(s) 683
BtsCI GGATG 5 cut(s) 18, 222, 692, 1811, 2427
BtsIMutI CAGTG 2 cut(s) 837, 1776
Cac8I GCNNGC 2 cut(s) 138, 2090
CaiI CAGNNNCTG 1 cut(s) 867
CciI TCATGA 1 cut(s) 2230
Cfr10I RCCGGY 1 cut(s) 148
Cfr13I GGNCC 6 cut(s) 48, 151, 202, 377, 673, 1225
CseI GACGC 2 cut(s) 2211, 2370
Csp6I GTAC 8 cut(s) 164, 588, 704, 750, 1022, 1687, 1943, 2014
CviQI GTAC 8 cut(s) 164, 588, 704, 750, 1022, 1687, 1943, 2014
DdeI CTNAG 8 cut(s) 178, 324, 366, 677, 936, 1471, 1787, 2130
DpnI GATC 8 cut(s) 240, 817, 1317, 1323, 1782, 1953, 2022, 2082
DpnII GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
DraI TTTAAA 1 cut(s) 2410
DrdI GACNNNNNNGTC 1 cut(s) 2240
DseDI GACNNNNNNGTC 1 cut(s) 2240
EaeI YGGCCR 1 cut(s) 2271
Eam1104I CTCTTC 2 cut(s) 12, 95
EarI CTCTTC 2 cut(s) 12, 95
EciI GGCGGA 1 cut(s) 135
Eco31I GGTCTC 4 cut(s) 102, 181, 325, 1722
Eco32I GATATC 1 cut(s) 637
Eco47I GGWCC 6 cut(s) 48, 151, 202, 377, 673, 1225
Eco57I CTGAAG 2 cut(s) 1171, 2342
Eco88I CYCGRG 1 cut(s) 2236
EcoNI CCTNNNNNAGG 1 cut(s) 257
EcoO109I RGGNCCY 4 cut(s) 48, 202, 377, 673
EcoRI GAATTC 2 cut(s) 1390, 1888
EcoRII CCWGG 7 cut(s) 258, 380, 1015, 1325, 1366, 1700, 1720
EcoRV GATATC 1 cut(s) 637
FaqI GGGAC 4 cut(s) 34, 137, 390, 2125
FauNDI CATATG 1 cut(s) 1747
FblI GTMKAC 1 cut(s) 1987
Fnu4HI GCNGC 4 cut(s) 301, 1481, 2034, 2259
FokI GGATG 5 cut(s) 5, 229, 699, 1818, 2434
Fsp4HI GCNGC 4 cut(s) 301, 1481, 2034, 2259
FspBI CTAG 6 cut(s) 137, 620, 812, 953, 1118, 1122
GluI GCNGC 4 cut(s) 301, 1481, 2034, 2259
GsaI CCCAGC 1 cut(s) 2088
GsuI CTGGAG 2 cut(s) 1350, 2001
HaeIII GGCC 2 cut(s) 1700, 2273
HapII CCGG 4 cut(s) 149, 422, 1801, 2337
HgaI GACGC 2 cut(s) 2211, 2370
HincII GTYRAC 2 cut(s) 1444, 1458
HindII GTYRAC 2 cut(s) 1444, 1458
HindIII AAGCTT 1 cut(s) 550
HinfI GANTC 9 cut(s) 778, 857, 968, 1467, 1508, 1592, 1847, 2101, 2234
HpaII CCGG 4 cut(s) 149, 422, 1801, 2337
HphI GGTGA 6 cut(s) 594, 656, 1115, 1509, 1631, 2160
Hpy188I TCNGA 8 cut(s) 44, 247, 325, 772, 820, 937, 2133, 2322
Hpy99I CGWCG 1 cut(s) 233
HpyCH4III ACNGT 8 cut(s) 175, 212, 841, 910, 1026, 1146, 1403, 1686
HpyCH4IV ACGT 4 cut(s) 194, 590, 682, 2061
HpyCH4V TGCA 7 cut(s) 105, 485, 580, 994, 1480, 1776, 2067
HpyF10VI GCNNNNNNNGC 1 cut(s) 287
HpyF3I CTNAG 8 cut(s) 178, 324, 366, 677, 936, 1471, 1787, 2130
HpySE526I ACGT 4 cut(s) 194, 590, 682, 2061
KflI GGGWCCC 1 cut(s) 377
Kzo9I GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 5 cut(s) 41, 1078, 1369, 1742, 1997
Lsp1109I GCAGC 4 cut(s) 312, 1492, 2020, 2245
LweI GCATC 6 cut(s) 268, 1115, 1228, 1306, 1416, 2412
MaeI CTAG 6 cut(s) 137, 620, 812, 953, 1118, 1122
MaeII ACGT 4 cut(s) 194, 590, 682, 2061
MaeIII GTNAC 2 cut(s) 2198, 2215
MalI GATC 8 cut(s) 240, 817, 1317, 1323, 1782, 1953, 2022, 2082
MboI GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
MflI RGATCY 4 cut(s) 815, 1315, 2020, 2080
MhlI GDGCHC 2 cut(s) 345, 1778
MlsI TGGCCA 1 cut(s) 2273
MluNI TGGCCA 1 cut(s) 2273
MlyI GAGTC 1 cut(s) 2228
MmeI TCCRAC 6 cut(s) 194, 246, 270, 331, 440, 1975
Mox20I TGGCCA 1 cut(s) 2273
MscI TGGCCA 1 cut(s) 2273
MseI TTAA 9 cut(s) 65, 465, 669, 711, 1554, 1665, 1878, 1929, 2409
MslI CAYNNNNRTG 7 cut(s) 711, 1044, 1205, 1600, 1659, 1737, 2075
Msp20I TGGCCA 1 cut(s) 2273
MspI CCGG 4 cut(s) 149, 422, 1801, 2337
MspR9I CCNGG 9 cut(s) 260, 382, 422, 1017, 1327, 1368, 1702, 1722, 1802
Mva1269I GAATGC 1 cut(s) 1191
MvaI CCWGG 7 cut(s) 260, 382, 1017, 1327, 1368, 1702, 1722
MwoI GCNNNNNNNGC 1 cut(s) 287
NciI CCSGG 2 cut(s) 422, 1802
NdeI CATATG 1 cut(s) 1747
NdeII GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
NheI GCTAGC 1 cut(s) 136
NlaIV GGNNCC 8 cut(s) 50, 153, 204, 342, 378, 379, 2049, 2082
NspI RCATGY 5 cut(s) 584, 710, 1210, 1456, 2295
PaeR7I CTCGAG 1 cut(s) 2236
PagI TCATGA 1 cut(s) 2230
PciI ACATGT 3 cut(s) 706, 1206, 2291
PciSI GCTCTTC 1 cut(s) 12
PctI GAATGC 1 cut(s) 1191
PfeI GAWTC 8 cut(s) 778, 857, 968, 1467, 1508, 1592, 1847, 2101
PkrI GCNGC 4 cut(s) 302, 1482, 2035, 2260
PleI GAGTC 1 cut(s) 2228
PpsI GAGTC 1 cut(s) 2228
Ppu21I YACGTR 1 cut(s) 591
PpuMI RGGWCCY 4 cut(s) 48, 202, 377, 673
PscI ACATGT 3 cut(s) 706, 1206, 2291
PsiI TTATAA 1 cut(s) 905
Psp5II RGGWCCY 4 cut(s) 48, 202, 377, 673
Psp6I CCWGG 7 cut(s) 258, 380, 1015, 1325, 1366, 1700, 1720
PspFI CCCAGC 1 cut(s) 2084
PspGI CCWGG 7 cut(s) 258, 380, 1015, 1325, 1366, 1700, 1720
PspN4I GGNNCC 8 cut(s) 50, 153, 204, 342, 378, 379, 2049, 2082
PspPI GGNCC 6 cut(s) 48, 151, 202, 377, 673, 1225
PspPPI RGGWCCY 4 cut(s) 48, 202, 377, 673
PspXI VCTCGAGB 1 cut(s) 2236
PstNI CAGNNNCTG 1 cut(s) 867
PsuI RGATCY 4 cut(s) 815, 1315, 2020, 2080
RsaI GTAC 8 cut(s) 165, 589, 705, 751, 1023, 1688, 1944, 2015
RsaNI GTAC 8 cut(s) 164, 588, 704, 750, 1022, 1687, 1943, 2014
RseI CAYNNNNRTG 7 cut(s) 711, 1044, 1205, 1600, 1659, 1737, 2075
SapI GCTCTTC 1 cut(s) 12
SaqAI TTAA 9 cut(s) 65, 465, 669, 711, 1554, 1665, 1878, 1929, 2409
SatI GCNGC 4 cut(s) 301, 1481, 2034, 2259
Sau3AI GATC 8 cut(s) 238, 815, 1315, 1321, 1780, 1951, 2020, 2080
Sau96I GGNCC 6 cut(s) 48, 151, 202, 377, 673, 1225
ScaI AGTACT 1 cut(s) 751
SchI GAGTC 1 cut(s) 2228
ScrFI CCNGG 9 cut(s) 260, 382, 422, 1017, 1327, 1368, 1702, 1722, 1802
SduI GDGCHC 2 cut(s) 345, 1778
SfaNI GCATC 6 cut(s) 268, 1115, 1228, 1306, 1416, 2412
Sfr274I CTCGAG 1 cut(s) 2236
SinI GGWCC 6 cut(s) 48, 151, 202, 377, 673, 1225
SlaI CTCGAG 1 cut(s) 2236
SmiMI CAYNNNNRTG 7 cut(s) 711, 1044, 1205, 1600, 1659, 1737, 2075
SmlI CTYRAG 3 cut(s) 2158, 2187, 2236
SmoI CTYRAG 3 cut(s) 2158, 2187, 2236
SpeI ACTAGT 1 cut(s) 952
SsiI CCGC 2 cut(s) 134, 146
SspI AATATT 1 cut(s) 1053
SspMI CTAG 6 cut(s) 137, 620, 812, 953, 1118, 1122
StyD4I CCNGG 9 cut(s) 258, 380, 420, 1015, 1325, 1366, 1700, 1720, 1800
TaaI ACNGT 8 cut(s) 175, 212, 841, 910, 1026, 1146, 1403, 1686
TaiI ACGT 4 cut(s) 197, 593, 685, 2064
TaqI TCGA 6 cut(s) 518, 595, 633, 1892, 2237, 2281
TaqII GACCGA 1 cut(s) 1704
TatI WGTACW 5 cut(s) 703, 749, 1021, 1686, 1942
TfiI GAWTC 8 cut(s) 778, 857, 968, 1467, 1508, 1592, 1847, 2101
Tru1I TTAA 9 cut(s) 65, 465, 669, 711, 1554, 1665, 1878, 1929, 2409
Tru9I TTAA 9 cut(s) 65, 465, 669, 711, 1554, 1665, 1878, 1929, 2409
TscAI CASTG 2 cut(s) 844, 1783
TseI GCWGC 4 cut(s) 300, 1480, 2033, 2258
TspGWI ACGGA 3 cut(s) 1243, 1477, 1749
TspRI CASTG 2 cut(s) 844, 1783
VneI GTGCAC 1 cut(s) 1774
VpaK11BI GGWCC 6 cut(s) 48, 151, 202, 377, 673, 1225
XagI CCTNNNNNAGG 1 cut(s) 257
XapI RAATTY 7 cut(s) 597, 919, 945, 1348, 1390, 1888, 2117
XceI RCATGY 5 cut(s) 584, 710, 1210, 1456, 2295
XcmI CCANNNNNNNNNTGG 1 cut(s) 868
XhoI CTCGAG 1 cut(s) 2236
XmiI GTMKAC 1 cut(s) 1987
XspI CTAG 6 cut(s) 137, 620, 812, 953, 1118, 1122
ZrmI AGTACT 1 cut(s) 751
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.