pycom13g06410

Belongs to the RNase T2 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013881
Physical Location & Seq
Reverse (-)
4156372 .. 4158445
2074 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGAGATACAGCAATACTTTGATCTTGATCAAGCTTTTGATCATTCAGTACCTCTCGGTTCTTTGTGTTTCTCAGGACTTTGATTTCTTCTACTTTGTTCAACAGTGGCCAGGAGCATACTGTGACACAAAACATACTTGTTGCTATCCCAAGTCAGGAAAGCCTACAGCAGATTTTGGCATTCATGGTCTATGGCCTAATTATAAGGATGGTGGCTACCCCTCTAACTGTGATCCCGACAGCGTCTTCGACAAATCTCAGATCTCAGAGCTGTTGACCAGTCTTAATAAGAACTGGCCATCACTGAGCTGCCCAAGTAGCAATGGTTACAGGTTCTGGTCACATGAATGGGAAAAGCACGGGACTTGCTCCGAGTCCGAGCTCGATCAGAAAGAGTACTTCGAAGCAGCCCTCAAACTCAGAGAAAAAGTTAAACTTCTACAAATTCTAAAAAATGCTGGAATCGTGCCAAATGATAAACTTTACAACCTAGAGAGCATAGTGGAAGCTATAAAAGAAGGTGTTGGGCACACCCCAGGGATAGAGTGCAACAAGGATTCGGCCGGAAACAGCCAACTTTACCAAATTTATTTGTGTGTCGACACTTCTGGCCAAGACATCATCGAGTGTCCTCTCCTTCCAAAGGGACGATGTGCTTCCAAAGTTCAATTCCCTAAATTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribonuclease_T2

Protein Analysis

228

Amino Acids

25.73

Weight (kDa)

5.96

Isoelectric Point (pI)

31.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribonuclease_T2 PF00445 27 - 210 6.8e-65 Ribonuclease T2 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013881)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G14220 AT1G26820
fragaria_vesca FvH4_4g31290
malus_domestica MD13G1071500.v1.1
prunus_persica Prupe.1G275400_v2.0.a1
pyrus_communis pycom13g06410
rosa_chinensis RchiOBHm_Chr4g0439731
rosa_laevigata RLG00000006241
rosa_multiflora Rmu_sc0010226.1_g000016 Rmu_ssc0000029.1_g000011
rosa_roxburghii Rroxscaffold_5G00380670
rosa_rugosa Rorug04G0321100
rosa_samantha Rh4AG372100 Rh4BG384200 Rh4CG398900 Rh4DG378600
rosa_wichuraiana Rw4G031770

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 204
AccI GTMKAC 1 cut(s) 600
AclWI GGATC 1 cut(s) 227
AcoI YGGCCR 4 cut(s) 107, 296, 561, 610
AcsI RAATTY 3 cut(s) 444, 585, 677
AfaI GTAC 2 cut(s) 50, 398
AfiI CCNNNNNNNGG 2 cut(s) 155, 643
AgsI TTSAA 2 cut(s) 101, 668
AjnI CCWGG 2 cut(s) 109, 535
AjuI GAANNNNNNNTTGG 1 cut(s) 653
AluBI AGCT 5 cut(s) 34, 271, 309, 382, 509
AluI AGCT 5 cut(s) 34, 271, 309, 382, 509
Alw21I GWGCWC 1 cut(s) 384
AlwI GGATC 1 cut(s) 227
AlwNI CAGNNNCTG 1 cut(s) 336
AoxI GGCC 5 cut(s) 107, 194, 296, 561, 610
ApeKI GCWGC 2 cut(s) 309, 407
ApoI RAATTY 3 cut(s) 444, 585, 677
ArsI GACNNNNNNTTYG 2 cut(s) 230, 262
AsuII TTCGAA 1 cut(s) 402
BaeGI GKGCMC 1 cut(s) 531
BalI TGGCCA 3 cut(s) 109, 298, 612
BanII GRGCYC 1 cut(s) 384
BbsI GAAGAC 1 cut(s) 238
Bbv12I GWGCWC 1 cut(s) 384
BbvI GCAGC 2 cut(s) 296, 419
BccI CCATC 2 cut(s) 203, 307
BciT130I CCWGG 2 cut(s) 111, 537
BclI TGATCA 2 cut(s) 27, 39
BfaI CTAG 1 cut(s) 491
BfmI CTRYAG 1 cut(s) 165
BglII AGATCT 1 cut(s) 261
BisI GCNGC 2 cut(s) 310, 408
BlsI GCNGC 2 cut(s) 311, 409
BmcAI AGTACT 1 cut(s) 398
Bme1390I CCNGG 2 cut(s) 111, 537
BmrFI CCNGG 2 cut(s) 111, 537
BpiI GAAGAC 1 cut(s) 238
Bpu14I TTCGAA 1 cut(s) 402
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 2 cut(s) 535, 536
Bsc4I CCNNNNNNNGG 2 cut(s) 155, 643
Bse1I ACTGG 2 cut(s) 279, 299
Bse3DI GCAATG 1 cut(s) 328
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 2 cut(s) 111, 537
BseDI CCNNGG 2 cut(s) 535, 536
BseGI GGATG 1 cut(s) 214
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 2 cut(s) 155, 643
BseMI GCAATG 1 cut(s) 328
BseMII CTCAG 5 cut(s) 86, 272, 279, 296, 433
BseNI ACTGG 2 cut(s) 279, 299
BseSI GKGCMC 1 cut(s) 531
BseX3I CGGCCG 1 cut(s) 561
BseXI GCAGC 2 cut(s) 296, 419
Bsh1285I CGRYCG 1 cut(s) 564
BshFI GGCC 5 cut(s) 109, 196, 298, 563, 612
BsiEI CGRYCG 1 cut(s) 564
BsiHKAI GWGCWC 1 cut(s) 384
BsiSI CCGG 1 cut(s) 564
BslFI GGGAC 2 cut(s) 376, 660
BslI CCNNNNNNNGG 2 cut(s) 155, 643
BsmFI GGGAC 2 cut(s) 376, 660
BsmI GAATGC 1 cut(s) 180
BsnI GGCC 5 cut(s) 109, 196, 298, 563, 612
Bsp119I TTCGAA 1 cut(s) 402
Bsp1286I GDGCHC 2 cut(s) 384, 531
Bsp143I GATC 6 cut(s) 21, 27, 39, 232, 261, 385
BspANI GGCC 5 cut(s) 109, 196, 298, 563, 612
BspCNI CTCAG 5 cut(s) 85, 271, 278, 297, 432
BspPI GGATC 1 cut(s) 227
BspT104I TTCGAA 1 cut(s) 402
BsrDI GCAATG 1 cut(s) 328
BsrI ACTGG 2 cut(s) 279, 299
BssECI CCNNGG 2 cut(s) 535, 536
BssMI GATC 6 cut(s) 21, 27, 39, 232, 261, 385
Bst2UI CCWGG 2 cut(s) 111, 537
Bst4CI ACNGT 3 cut(s) 105, 122, 230
BstBI TTCGAA 1 cut(s) 402
BstDEI CTNAG 5 cut(s) 72, 258, 265, 305, 419
BstENI CCTNNNNNAGG 1 cut(s) 641
BstF5I GGATG 1 cut(s) 214
BstKTI GATC 6 cut(s) 24, 30, 42, 235, 264, 388
BstMBI GATC 6 cut(s) 21, 27, 39, 232, 261, 385
BstMCI CGRYCG 1 cut(s) 564
BstMWI GCNNNNNNNGC 1 cut(s) 318
BstNI CCWGG 2 cut(s) 111, 537
BstSCI CCNGG 2 cut(s) 109, 535
BstSFI CTRYAG 1 cut(s) 165
BstSLI GKGCMC 1 cut(s) 531
BstV1I GCAGC 2 cut(s) 296, 419
BstV2I GAAGAC 1 cut(s) 238
BstX2I RGATCY 1 cut(s) 261
BstYI RGATCY 1 cut(s) 261
BstZI CGGCCG 1 cut(s) 561
BsuRI GGCC 5 cut(s) 109, 196, 298, 563, 612
BtsCI GGATG 1 cut(s) 214
BtsIMutI CAGTG 2 cut(s) 110, 302
CaiI CAGNNNCTG 1 cut(s) 336
CseI GACGC 1 cut(s) 232
Csp6I GTAC 2 cut(s) 49, 397
CviAII CATG 2 cut(s) 185, 344
CviQI GTAC 2 cut(s) 49, 397
DdeI CTNAG 5 cut(s) 72, 258, 265, 305, 419
DpnI GATC 6 cut(s) 23, 29, 41, 234, 263, 387
DpnII GATC 6 cut(s) 21, 27, 39, 232, 261, 385
EaeI YGGCCR 4 cut(s) 107, 296, 561, 610
EagI CGGCCG 1 cut(s) 561
Ecl136II GAGCTC 1 cut(s) 382
EclXI CGGCCG 1 cut(s) 561
Eco24I GRGCYC 1 cut(s) 384
Eco52I CGGCCG 1 cut(s) 561
Eco53kI GAGCTC 1 cut(s) 382
EcoICRI GAGCTC 1 cut(s) 382
EcoNI CCTNNNNNAGG 1 cut(s) 641
EcoRII CCWGG 2 cut(s) 109, 535
EcoT38I GRGCYC 1 cut(s) 384
FaeI CATG 2 cut(s) 188, 347
FaiI YATR 8 cut(s) 118, 135, 186, 193, 204, 345, 500, 512
FalI AAGNNNNNCTT 2 cut(s) 420, 452
FaqI GGGAC 2 cut(s) 376, 660
FatI CATG 2 cut(s) 184, 343
FbaI TGATCA 2 cut(s) 27, 39
FblI GTMKAC 1 cut(s) 600
Fnu4HI GCNGC 2 cut(s) 310, 408
FokI GGATG 1 cut(s) 221
FriOI GRGCYC 1 cut(s) 384
Fsp4HI GCNGC 2 cut(s) 310, 408
FspBI CTAG 1 cut(s) 491
GluI GCNGC 2 cut(s) 310, 408
HaeIII GGCC 5 cut(s) 109, 196, 298, 563, 612
HapII CCGG 1 cut(s) 564
HgaI GACGC 1 cut(s) 232
Hin1II CATG 2 cut(s) 188, 347
HincII GTYRAC 2 cut(s) 276, 601
HindII GTYRAC 2 cut(s) 276, 601
HindIII AAGCTT 1 cut(s) 32
HinfI GANTC 3 cut(s) 374, 462, 557
HpaII CCGG 1 cut(s) 564
Hpy166II GTNNAC 2 cut(s) 276, 601
Hpy188I TCNGA 6 cut(s) 261, 268, 373, 379, 390, 422
Hpy188III TCNNGA 4 cut(s) 25, 74, 156, 236
Hpy8I GTNNAC 2 cut(s) 276, 601
HpyAV CCTTC 2 cut(s) 512, 647
HpyCH4III ACNGT 3 cut(s) 105, 122, 230
HpyCH4V TGCA 1 cut(s) 549
HpyF10VI GCNNNNNNNGC 1 cut(s) 318
HpyF3I CTNAG 5 cut(s) 72, 258, 265, 305, 419
Hsp92II CATG 2 cut(s) 188, 347
Ksp22I TGATCA 2 cut(s) 27, 39
Kzo9I GATC 6 cut(s) 21, 27, 39, 232, 261, 385
LmnI GCTCC 2 cut(s) 113, 374
Lsp1109I GCAGC 2 cut(s) 296, 419
MaeI CTAG 1 cut(s) 491
MaeIII GTNAC 3 cut(s) 122, 326, 339
MalI GATC 6 cut(s) 23, 29, 41, 234, 263, 387
MboI GATC 6 cut(s) 21, 27, 39, 232, 261, 385
MboII GAAGA 2 cut(s) 79, 238
MflI RGATCY 1 cut(s) 261
MhlI GDGCHC 2 cut(s) 384, 531
MlsI TGGCCA 3 cut(s) 109, 298, 612
MluCI AATT 5 cut(s) 199, 444, 585, 668, 677
MluNI TGGCCA 3 cut(s) 109, 298, 612
MlyI GAGTC 1 cut(s) 383
MnlI CCTC 4 cut(s) 62, 232, 422, 642
Mox20I TGGCCA 3 cut(s) 109, 298, 612
MscI TGGCCA 3 cut(s) 109, 298, 612
MseI TTAA 3 cut(s) 285, 432, 682
MslI CAYNNNNRTG 1 cut(s) 346
Msp20I TGGCCA 3 cut(s) 109, 298, 612
MspI CCGG 1 cut(s) 564
MspR9I CCNGG 2 cut(s) 111, 537
Mva1269I GAATGC 1 cut(s) 180
MvaI CCWGG 2 cut(s) 111, 537
MwoI GCNNNNNNNGC 1 cut(s) 318
NdeII GATC 6 cut(s) 21, 27, 39, 232, 261, 385
NlaIII CATG 2 cut(s) 188, 347
NmuCI GTSAC 2 cut(s) 122, 339
NspV TTCGAA 1 cut(s) 402
PasI CCCWGGG 1 cut(s) 536
PctI GAATGC 1 cut(s) 180
PfeI GAWTC 2 cut(s) 462, 557
PflFI GACNNNGTC 1 cut(s) 242
PkrI GCNGC 2 cut(s) 311, 409
PleI GAGTC 1 cut(s) 382
PpsI GAGTC 1 cut(s) 382
PsiI TTATAA 1 cut(s) 204
Psp124BI GAGCTC 1 cut(s) 384
Psp6I CCWGG 2 cut(s) 109, 535
PspGI CCWGG 2 cut(s) 109, 535
PstNI CAGNNNCTG 1 cut(s) 336
PsuI RGATCY 1 cut(s) 261
PsyI GACNNNGTC 1 cut(s) 242
RsaI GTAC 2 cut(s) 50, 398
RsaNI GTAC 2 cut(s) 49, 397
RseI CAYNNNNRTG 1 cut(s) 346
SacI GAGCTC 1 cut(s) 384
SalI GTCGAC 1 cut(s) 599
SaqAI TTAA 3 cut(s) 285, 432, 682
SatI GCNGC 2 cut(s) 310, 408
Sau3AI GATC 6 cut(s) 21, 27, 39, 232, 261, 385
ScaI AGTACT 1 cut(s) 398
SchI GAGTC 1 cut(s) 383
ScrFI CCNGG 2 cut(s) 111, 537
SduI GDGCHC 2 cut(s) 384, 531
SetI ASST 9 cut(s) 36, 54, 273, 311, 335, 384, 492, 511, 523
SfcI CTRYAG 1 cut(s) 165
SfuI TTCGAA 1 cut(s) 402
SmiMI CAYNNNNRTG 1 cut(s) 346
Sse9I AATT 5 cut(s) 199, 444, 585, 668, 677
SspMI CTAG 1 cut(s) 491
SstI GAGCTC 1 cut(s) 384
StyD4I CCNGG 2 cut(s) 109, 535
TaaI ACNGT 3 cut(s) 105, 122, 230
TaqI TCGA 5 cut(s) 249, 384, 402, 600, 624
TasI AATT 5 cut(s) 199, 444, 585, 668, 677
TatI WGTACW 1 cut(s) 396
TfiI GAWTC 2 cut(s) 462, 557
Tru1I TTAA 3 cut(s) 285, 432, 682
Tru9I TTAA 3 cut(s) 285, 432, 682
TscAI CASTG 2 cut(s) 110, 309
TseFI GTSAC 2 cut(s) 122, 339
TseI GCWGC 2 cut(s) 309, 407
Tsp45I GTSAC 2 cut(s) 122, 339
TspDTI ATGAA 2 cut(s) 173, 360
TspRI CASTG 2 cut(s) 110, 309
Tth111I GACNNNGTC 1 cut(s) 242
XagI CCTNNNNNAGG 1 cut(s) 641
XapI RAATTY 3 cut(s) 444, 585, 677
XmiI GTMKAC 1 cut(s) 600
XspI CTAG 1 cut(s) 491
ZrmI AGTACT 1 cut(s) 398
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.