pycom13g11420

Uncharacterized integral membrane protein (DUF2301)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0013259
Physical Location & Seq
Forward (+)
7852930 .. 7855181
2252 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1050 bp
ATGTGTGCCTATGTTAAGCTAAAGCCTTCATCATCTGCATCTGTGACAGCCACACATCTGAAAGTTCTGAACAGAGCGAATGGGATTTTTATACCATCAAGTAATCAAATAACTGAAATTAGCAGACCCGTTCCATTAATTTGCCCAAAAATGGGTTCCAGAATGCTGTGTCTGTCTGCAACCATCGTCCCTCCTCCTGCGCCTGCTCCTGCTGTAAAGCTTACCACATCAAATAATATGTCCCCGATTTTTCTTTCTTCTTCTGCAGATAAAGCTTCGGCTCGGCTTCAGTCTTTCAGGTGCAGAGCTGTCGGAGAAAGCTCTCAGCCGCAGACCTCGTTGGCTGAAACCGTATATCAGGGAGTTTACGGTCCTTGGTCCGTCGACTCCACCGACGTCAGAGAGGTTATTTTGTACAGATCGGGACTAGTGACTGCGGCTACATCATTTGTAGTTACAGCTTCTGCTGCATTTTTGCCTGATAGTTTCTTCTTAACTGACATTGTCAAACACAATCTCGATTTTGTCTACGCAATCGGAGCTGGTGGATTGGGGTTGTCCCTTTTTCTGATTCACATCTATGTGACTGAAATTAAGCGCACTCTGCAAGCATTTTGGGGGCTTGGTGTTGCCGGGTCTTTCGCAACATATGCATGCTTGGCTAGACCAGCTGGAGATGGTTTGGTGCAATATGTTGTGGATAACCCCGTCGCTATCTGGTTTGTTGGTCCTCTCTTTGCTTCTCTCACGGGACTAGTCTTCAAGGAAGGTCTCTGCTATGGAAAGTTGGAAGCTGGAATTCTCACATTCATCATACCTACTGTTCTTCTGGGGCACTTGAGTGGACTGATGGACGATGGAGCAAAACTTTCTTTGCTCGGTTTGTGGATGGCCCTGTTTGTAATTTTTGCCGGAAGGAAGTTCACTCAACCGATCAAGGATGACATTGGCGACAAGTCCGTCTTTACATTCAATTCTCTACCTGAAGACGAGAAGATGGCCTTGATTCAGAAGCTTGAGCAACAAAAGTTAAGTCAGGATGTTGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF2301

Protein Analysis

350

Amino Acids

37.51

Weight (kDa)

8.15

Isoelectric Point (pI)

41.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF2301 PF10063 186 - 324 2.2e-53 Uncharacterized integral membrane protein (DUF2301)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013259)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G28140
fragaria_vesca FvH4_4g22640 FvH4_4g22640
malus_domestica MD13G1130900.v1.1
prunus_persica Prupe.1G214800_v2.0.a1
pyrus_communis pycom13g11420 pycom16g11150
rosa_chinensis RchiOBHm_Chr4g0428871
rosa_laevigata RLG00000007118
rosa_multiflora Rmu_co8417267.1_g000001 Rmu_sc0008612.1_g000007
rosa_roxburghii Rroxscaffold_5G00370550
rosa_rugosa Rorug04G0229900
rosa_samantha Rh4AG286600 Rh4BG292400 Rh4CG308400 Rh4DG289600
rosa_wichuraiana Rw4G024850

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 959
AatII GACGTC 1 cut(s) 399
AccI GTMKAC 2 cut(s) 384, 528
AciI CCGC 2 cut(s) 329, 437
AcsI RAATTY 1 cut(s) 798
AcuI CTGAAG 2 cut(s) 272, 1005
AcyI GRCGYC 1 cut(s) 396
AfaI GTAC 1 cut(s) 416
AfiI CCNNNNNNNGG 2 cut(s) 151, 152
AgsI TTSAA 2 cut(s) 763, 973
AhlI ACTAGT 2 cut(s) 427, 754
AjuI GAANNNNNNNTTGG 2 cut(s) 139, 171
AleI CACNNNNGTG 1 cut(s) 840
Alw26I GTCTC 1 cut(s) 776
AlwNI CAGNNNCTG 1 cut(s) 464
AoxI GGCC 2 cut(s) 891, 999
ApeKI GCWGC 1 cut(s) 467
ApoI RAATTY 1 cut(s) 798
ArsI GACNNNNNNTTYG 4 cut(s) 224, 256, 1018, 1050
AseI ATTAAT 1 cut(s) 137
AspLEI GCGC 2 cut(s) 202, 600
AspS9I GGNCC 4 cut(s) 371, 378, 728, 892
AsuC2I CCSGG 1 cut(s) 634
AvaII GGWCC 3 cut(s) 371, 378, 728
BaeGI GKGCMC 1 cut(s) 837
BbsI GAAGAC 2 cut(s) 751, 993
BbvI GCAGC 1 cut(s) 454
BccI CCATC 7 cut(s) 103, 191, 671, 844, 851, 883, 991
BcgI CGANNNNNNTGC 2 cut(s) 292, 326
BcnI CCSGG 1 cut(s) 634
BcoDI GTCTC 1 cut(s) 776
BcuI ACTAGT 2 cut(s) 427, 754
BfaI CTAG 3 cut(s) 428, 663, 755
BfmI CTRYAG 1 cut(s) 264
BisI GCNGC 3 cut(s) 329, 438, 468
BlsI GCNGC 3 cut(s) 330, 439, 469
Bme1390I CCNGG 1 cut(s) 634
Bme18I GGWCC 3 cut(s) 371, 378, 728
BmgT120I GGNCC 4 cut(s) 371, 378, 728, 892
BmiI GGNNCC 1 cut(s) 157
BmrFI CCNGG 1 cut(s) 634
BmsI GCATC 1 cut(s) 47
BpiI GAAGAC 2 cut(s) 751, 993
BpmI CTGGAG 1 cut(s) 693
BpuEI CTTGAG 2 cut(s) 859, 1037
BpuMI CCSGG 1 cut(s) 634
BsaBI GATNNNNATC 1 cut(s) 575
BsaHI GRCGYC 1 cut(s) 396
BsaI GGTCTC 1 cut(s) 776
BsaJI CCNNGG 1 cut(s) 374
Bsc4I CCNNNNNNNGG 2 cut(s) 151, 152
Bse8I GATNNNNATC 1 cut(s) 575
BseDI CCNNGG 1 cut(s) 374
BseGI GGATG 3 cut(s) 894, 946, 1045
BseJI GATNNNNATC 1 cut(s) 575
BseLI CCNNNNNNNGG 2 cut(s) 151, 152
BseMII CTCAG 1 cut(s) 338
BseRI GAGGAG 1 cut(s) 183
BseSI GKGCMC 1 cut(s) 837
BseXI GCAGC 1 cut(s) 454
BsgI GTGCAG 1 cut(s) 322
BshFI GGCC 2 cut(s) 893, 1001
BsiSI CCGG 2 cut(s) 633, 912
BslFI GGGAC 5 cut(s) 173, 226, 438, 544, 765
BslI CCNNNNNNNGG 2 cut(s) 151, 152
BsmAI GTCTC 1 cut(s) 776
BsmFI GGGAC 5 cut(s) 173, 226, 438, 544, 765
BsmI GAATGC 1 cut(s) 168
BsnI GGCC 2 cut(s) 893, 1001
Bso31I GGTCTC 1 cut(s) 776
Bsp1286I GDGCHC 1 cut(s) 837
Bsp1407I TGTACA 1 cut(s) 414
Bsp143I GATC 2 cut(s) 419, 933
BspACI CCGC 2 cut(s) 329, 437
BspANI GGCC 2 cut(s) 893, 1001
BspCNI CTCAG 1 cut(s) 337
BspLI GGNNCC 1 cut(s) 157
BspMAI CTGCAG 1 cut(s) 268
BspTNI GGTCTC 1 cut(s) 776
BsrGI TGTACA 1 cut(s) 414
BssECI CCNNGG 1 cut(s) 374
BssMI GATC 2 cut(s) 419, 933
BssNI GRCGYC 1 cut(s) 396
BssT1I CCWWGG 1 cut(s) 374
Bst4CI ACNGT 3 cut(s) 352, 371, 823
BstACI GRCGYC 1 cut(s) 396
BstAPI GCANNNNNTGC 1 cut(s) 650
BstAUI TGTACA 1 cut(s) 414
BstC8I GCNNGC 3 cut(s) 204, 609, 655
BstDEI CTNAG 1 cut(s) 324
BstF5I GGATG 3 cut(s) 894, 946, 1045
BstHHI GCGC 2 cut(s) 202, 600
BstKTI GATC 2 cut(s) 422, 936
BstMAI GTCTC 1 cut(s) 776
BstMBI GATC 2 cut(s) 419, 933
BstMWI GCNNNNNNNGC 7 cut(s) 272, 467, 539, 604, 650, 659, 668
BstNSI RCATGY 1 cut(s) 657
BstSCI CCNGG 1 cut(s) 632
BstSFI CTRYAG 1 cut(s) 264
BstSLI GKGCMC 1 cut(s) 837
BstV1I GCAGC 1 cut(s) 454
BstV2I GAAGAC 2 cut(s) 751, 993
BsuRI GGCC 2 cut(s) 893, 1001
BtsCI GGATG 3 cut(s) 894, 946, 1045
Cac8I GCNNGC 3 cut(s) 204, 609, 655
CaiI CAGNNNCTG 1 cut(s) 464
CfoI GCGC 2 cut(s) 202, 600
Cfr13I GGNCC 4 cut(s) 371, 378, 728, 892
Csp6I GTAC 1 cut(s) 415
CviAII CATG 1 cut(s) 654
CviQI GTAC 1 cut(s) 415
DdeI CTNAG 1 cut(s) 324
DpnI GATC 2 cut(s) 421, 935
DpnII GATC 2 cut(s) 419, 933
DrdI GACNNNNNNGTC 1 cut(s) 959
DseDI GACNNNNNNGTC 1 cut(s) 959
Eco130I CCWWGG 1 cut(s) 374
Eco31I GGTCTC 1 cut(s) 776
Eco47I GGWCC 3 cut(s) 371, 378, 728
Eco57I CTGAAG 2 cut(s) 272, 1005
EcoRI GAATTC 1 cut(s) 798
EcoT14I CCWWGG 1 cut(s) 374
EcoT22I ATGCAT 1 cut(s) 655
ErhI CCWWGG 1 cut(s) 374
FaeI CATG 1 cut(s) 657
FalI AAGNNNNNCTT 4 cut(s) 947, 979, 986, 1018
FaqI GGGAC 5 cut(s) 173, 226, 438, 544, 765
FatI CATG 1 cut(s) 653
FauNDI CATATG 1 cut(s) 649
FblI GTMKAC 2 cut(s) 384, 528
Fnu4HI GCNGC 3 cut(s) 329, 438, 468
FokI GGATG 2 cut(s) 901, 953
Fsp4HI GCNGC 3 cut(s) 329, 438, 468
FspBI CTAG 3 cut(s) 428, 663, 755
GlaI GCGC 2 cut(s) 201, 599
GluI GCNGC 3 cut(s) 329, 438, 468
GsuI CTGGAG 1 cut(s) 693
HaeIII GGCC 2 cut(s) 893, 1001
HapII CCGG 2 cut(s) 633, 912
HhaI GCGC 2 cut(s) 202, 600
Hin1I GRCGYC 1 cut(s) 396
Hin1II CATG 1 cut(s) 657
Hin6I GCGC 2 cut(s) 200, 598
HinP1I GCGC 2 cut(s) 200, 598
HincII GTYRAC 1 cut(s) 385
HindII GTYRAC 1 cut(s) 385
HindIII AAGCTT 3 cut(s) 218, 273, 1013
HinfI GANTC 3 cut(s) 386, 571, 1006
HpaII CCGG 2 cut(s) 633, 912
Hpy166II GTNNAC 5 cut(s) 367, 385, 529, 845, 924
Hpy188I TCNGA 7 cut(s) 60, 69, 314, 401, 539, 570, 1011
Hpy188III TCNNGA 4 cut(s) 159, 423, 518, 1037
Hpy8I GTNNAC 5 cut(s) 367, 385, 529, 845, 924
Hpy99I CGWCG 3 cut(s) 386, 398, 713
HpyAV CCTTC 3 cut(s) 36, 761, 909
HpyCH4III ACNGT 3 cut(s) 352, 371, 823
HpyCH4IV ACGT 1 cut(s) 396
HpyCH4V TGCA 8 cut(s) 38, 179, 266, 303, 470, 607, 653, 688
HpyF10VI GCNNNNNNNGC 7 cut(s) 272, 467, 539, 604, 650, 659, 668
HpyF3I CTNAG 1 cut(s) 324
HpySE526I ACGT 1 cut(s) 396
Hsp92I GRCGYC 1 cut(s) 396
Hsp92II CATG 1 cut(s) 657
HspAI GCGC 2 cut(s) 200, 598
Kzo9I GATC 2 cut(s) 419, 933
LmnI GCTCC 3 cut(s) 211, 539, 860
Lsp1109I GCAGC 1 cut(s) 454
LweI GCATC 1 cut(s) 47
MaeI CTAG 3 cut(s) 428, 663, 755
MaeII ACGT 1 cut(s) 396
MaeIII GTNAC 4 cut(s) 43, 430, 454, 583
MalI GATC 2 cut(s) 421, 935
MboI GATC 2 cut(s) 419, 933
MboII GAAGA 7 cut(s) 249, 252, 481, 751, 818, 998, 1006
MhlI GDGCHC 1 cut(s) 837
MluCI AATT 6 cut(s) 117, 138, 591, 798, 903, 973
MlyI GAGTC 1 cut(s) 380
MmeI TCCRAC 2 cut(s) 292, 768
MnlI CCTC 5 cut(s) 201, 204, 346, 397, 741
Mph1103I ATGCAT 1 cut(s) 655
MseI TTAA 5 cut(s) 15, 137, 494, 594, 1031
MslI CAYNNNNRTG 4 cut(s) 579, 581, 652, 840
MspA1I CMGCKG 1 cut(s) 671
MspI CCGG 2 cut(s) 633, 912
MspR9I CCNGG 1 cut(s) 634
Mva1269I GAATGC 1 cut(s) 168
MwoI GCNNNNNNNGC 7 cut(s) 272, 467, 539, 604, 650, 659, 668
NciI CCSGG 1 cut(s) 634
NdeI CATATG 1 cut(s) 649
NdeII GATC 2 cut(s) 419, 933
NlaIII CATG 1 cut(s) 657
NlaIV GGNNCC 1 cut(s) 157
NmeAIII GCCGAG 1 cut(s) 262
NmuCI GTSAC 3 cut(s) 43, 430, 583
NsiI ATGCAT 1 cut(s) 655
NspI RCATGY 1 cut(s) 657
OliI CACNNNNGTG 1 cut(s) 840
PaeI GCATGC 1 cut(s) 657
PcsI WCGNNNNNNNCGW 1 cut(s) 390
PctI GAATGC 1 cut(s) 168
PfeI GAWTC 2 cut(s) 571, 1006
PflFI GACNNNGTC 1 cut(s) 503
PkrI GCNGC 3 cut(s) 330, 439, 469
PleI GAGTC 1 cut(s) 380
PpsI GAGTC 1 cut(s) 380
PshBI ATTAAT 1 cut(s) 137
PspN4I GGNNCC 1 cut(s) 157
PspPI GGNCC 4 cut(s) 371, 378, 728, 892
PstI CTGCAG 1 cut(s) 268
PstNI CAGNNNCTG 1 cut(s) 464
PsyI GACNNNGTC 1 cut(s) 503
PvuII CAGCTG 1 cut(s) 671
RsaI GTAC 1 cut(s) 416
RsaNI GTAC 1 cut(s) 415
RseI CAYNNNNRTG 4 cut(s) 579, 581, 652, 840
SalI GTCGAC 1 cut(s) 383
SaqAI TTAA 5 cut(s) 15, 137, 494, 594, 1031
SatI GCNGC 3 cut(s) 329, 438, 468
Sau3AI GATC 2 cut(s) 419, 933
Sau96I GGNCC 4 cut(s) 371, 378, 728, 892
SchI GAGTC 1 cut(s) 380
ScrFI CCNGG 1 cut(s) 634
SduI GDGCHC 1 cut(s) 837
SfaNI GCATC 1 cut(s) 47
SfcI CTRYAG 1 cut(s) 264
SinI GGWCC 3 cut(s) 371, 378, 728
SmiMI CAYNNNNRTG 4 cut(s) 579, 581, 652, 840
SmlI CTYRAG 2 cut(s) 838, 1016
SmoI CTYRAG 2 cut(s) 838, 1016
SpeI ACTAGT 2 cut(s) 427, 754
SphI GCATGC 1 cut(s) 657
Sse9I AATT 6 cut(s) 117, 138, 591, 798, 903, 973
SsiI CCGC 2 cut(s) 329, 437
SspMI CTAG 3 cut(s) 428, 663, 755
StyD4I CCNGG 1 cut(s) 632
StyI CCWWGG 1 cut(s) 374
TaaI ACNGT 3 cut(s) 352, 371, 823
TaiI ACGT 1 cut(s) 399
TaqI TCGA 2 cut(s) 384, 519
TasI AATT 6 cut(s) 117, 138, 591, 798, 903, 973
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 331, 440
TfiI GAWTC 2 cut(s) 571, 1006
Tru1I TTAA 5 cut(s) 15, 137, 494, 594, 1031
Tru9I TTAA 5 cut(s) 15, 137, 494, 594, 1031
TseFI GTSAC 3 cut(s) 43, 430, 583
TseI GCWGC 1 cut(s) 467
Tsp45I GTSAC 3 cut(s) 43, 430, 583
TspDTI ATGAA 2 cut(s) 18, 799
TspGWI ACGGA 2 cut(s) 370, 949
Tth111I GACNNNGTC 1 cut(s) 503
VpaK11BI GGWCC 3 cut(s) 371, 378, 728
VspI ATTAAT 1 cut(s) 137
XapI RAATTY 1 cut(s) 798
XceI RCATGY 1 cut(s) 657
XmiI GTMKAC 2 cut(s) 384, 528
XspI CTAG 3 cut(s) 428, 663, 755
ZraI GACGTC 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.