pycom13g11900

GPI ethanolamine phosphate transferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0009386
Physical Location & Seq
Forward (+)
8203949 .. 8211105
7157 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3222 bp
ATGCAGAGAAAAAAAAAATCTCAATTTGGCAAAATAATTGACATAATTAATAGAACTATCCACGAGTCCGTTTTTGGAAATAAATTTAAAAAAAAAAAGTGCCAAAAGAAGGGAGTGCGAGAAGCAGCCGGAGCAAGAAAAAATGCAAACGCCAGTGCTGTTGCAAGATGGAAGTCATGCACTGAATCAAACGCCATCAACGCGCGGCAATCACTGCCCTTAAAATTCAAGATCCCACAAGAAACCCAGAAACCAGCATCCCTTTTCTTCTTCTTCTTCTTCTTTTTTCCTGCAATTACGTGCAAGATGGGAAAACGAATATGGATTCCCTGGGCGATCATGGCGCTCCACGTCGTCGCCATTCTCTTATTCACCAGAGGCTTTCTTCTCACCCGGACGGAACTTCCATTTTACAGCAACTGCTCCGACGGTTCGCAGTCGCCTTGCTCCTACTCCGTTCCAGAAACCCAGAACCGAAACGACACCGTTGACCCGAATCAGCAGCGTTGCTGGAGCAAGCCTGCTGTTGGGCGCCTTGTAATCATCGTTTTCGACGCCCTCAGGTTTGATTTTGTGGCTCCCAGTACTTTCTTTCAGGAATCAAAACCATGGATGGACAAATTAAAACTTGTTCAAGACATGGCAGCCAGAAATGCGTCGACCGCTCGGATTTTTAAAGCCATTGCTGATCCGCCAACAACGAGTTTGCAGCGTTTGAAGGGCTTGACAACCGGTGGATTGCCGACTTTTATTGATGTTGGGAACAGCTTTGGTGCTCCAGCAATAGTGGAAGATAACTTGATACATCAGTTGGCTAAAAATGGAAAGCGAGTTGTAATGATGGGGGACGATACATGGACGCAGTTGTTTCCTCGTCATTTTGAAAAATCTTTTCCTTACCCCTCTTTCAATGTTAGAGATCTTGATACGGTGGACAATGGCTGCATTGAGCACCTTCTTCCATTCTTGTACCAAGAAGATTGGGATGTTCTTATCGCACACTTTCTTGGTGTGGATCACGCTGGGCACATATTTGGGGTTGATTCTATGCAAATGATAGAAAAGTTGGAGCAATACAACAACGTCTTGCTGAAAGTAGTTGAAGCACTGGAGAGGCAGTCTGCGCCAGGGGGCTTACATGAGAATACTCTTTTACTTGTGATGGGCGATCATGGACAGACTGTGAATGGAGATCATGGTGGGGGGAGTTCTGAAGAGGTTGAGACATCATTATTTGCTGTGAGCTTTAAGAATCCTCCATCTCCCATACCATCAGAGTTTGATACTTCATCTTGTGAACTTGATTTGGATGGGAGGAATATTTGCACAAGTTCCATGCAGCAGCTTGACTTTGCAGTGACAATATCAGCGCTGCTTGGTATCCCCTTTCCTTTTGGAAGTATTGGGCGTGTTAATCCTCAGCTTTATGCTTTAGGAGTTGGAACATGGAATTTTGAAGATAGTGTGGGCAATTCCCAAAATCAGTCAAAGTTGGAACAATGGATGCTGAATTATGCCAATGTACTCTGCACCAATTCTTGGCAGGTAAAAAGATATATAGATATATATTCTGCTTCATCAGTTATTGGATTTTCCCATGAAGATTTGTTGCACATAGCAGACATGTATGCTAAAGCAGAGGAGAGATGGTCACTTACTACAAAGAAGGTGCTGTCACACGAAAAGGAAAGCCACAATGAATTATTGCCTGCTCTTAAGAGGCAAATTAATTTGTATTCTGATTTCCTAGCAAGTGTTGCTGAACTTGCACGCTCTAAATGGACTGAGTTTAATTTAAAGATGATGGGTGCTGGACTTGGCATTATGCTGATATCACTTCTTGTCCATTTCCTTGCAATTAAGAAGGTGAAGGAGGAATATGGTTTTTCTTTTTCTTCATCTGGAGATTCTGGGATTTCCTTCAGTTTAATTTTTTCTTGTTTTATGGTGGTGATGCGTGCGTGCAGCTTCCTCTCGAATAGTTTTATCTTGGAAGAAGGAAAAGTGGCATGCTTTCTTTTGGCCACTACTGGACTAATTAAGATGCGATATTCAATCATGAAGAAGAAGATGATATTAGAAGCACTTGTTTTCCTTCTTCTGATCACCATTTGCAGATTTACTATTGAAGTTGGATTGTCAAAGCAGGCTCCTAGTTCTGAAATTATTAATGCATATCCTTCATGGATGCTCAGGATCACCGCAGGGTTTCCAGTTTGGAATATTGTTGCTGAAGTTATACCGGCAGTTTCCCTGATTTTATTGGCATTTTTGCTGCGCAAGGCCATTACTGGAAGCTCTTCCGAGGGGATTTGGAAGTATATTATCATGGGAACTAATATAAGTTATATACTAATAGCAGTGCATTGGGCTTCAGAAAGTAACATATTCAATCTGGCCGAGGTGCTTAAAGGCATAGGAAGAAATTACATTCCTCGTCTGATATATGCCATTGGATTTGGACAATTGTTATTACTGGCATTTAATCAGCTTTTCAATAAAGGGACATCCTCAGATTGCAGCAAAGTCTTATATATTAAAACAGTGGCCATGTTTTCTGCCTGGAGCTCAACACTCATTCTTCTGTTGGGAAAGCAGGGTCCTTGGATTGCTTTAGCATTCGTTATTGGAGGTTATTGCATAATGAGGCTGGATAACATAGAGCTGGATGCCAAAGATGGAGGCAGTTGGAATACGATGCTTGATCCTATTCCTGTAACGCAGTGGAGCCTTTTTGCTGTGTGCCTGTTTTTCTGCACCGGTCATTGGTGTGCTTTTGACGGCCTCCGATATGGTGCTGCATTTATTGGGTTCGATGATTTCATCCTAGTTCCCCAAGCAATCCTCCTTACAATGGATACATTTGGTTTTTCTCTCATCCTTCCAATATTTGGACTTCCATTTCTTGTTGCACGCCTGGGTCAGACTGAGAAAGGGAAGAAATTTGTGCTCACACGACTATCTCTAGTGTACATGATTTATGGGCTCATCATGGCTACTTCGGTCACAGCAACTATAATATGTGTTACAATGCACAGGCGGCATCTAATGGTATGGGGTTTATTTGCCCCGAAGTTTGTTTTCGATGTGGCGGGTCTTATCCTTACAGACATACTCGTTTGTTTGGCCTCACATTACTACTTCAGTCAAGTAGAAGACGATGCCCTACAAGATCCGTTCACCAAAGACAATGCCCTACAAGATCTGTTCACCAAAAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00563 ko01100 map00563 map01100
Pfam Domains
Protein Families
Phosphodiest

Protein Analysis

1074

Amino Acids

120.07

Weight (kDa)

8.52

Isoelectric Point (pI)

42.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Phosphodiest PF01663 311 - 394 2.2e-09 Type I phosphodiesterase / nucleotide pyrophosphatase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 2279
Acc36I ACCTGC 1 cut(s) 1534
AccB1I GGYRCC 1 cut(s) 531
AccB7I CCANNNNNTGG 2 cut(s) 1539, 2891
AccBSI CCGCTC 1 cut(s) 665
AccI GTMKAC 1 cut(s) 659
AccII CGCG 2 cut(s) 203, 205
AciI CCGC 6 cut(s) 205, 663, 692, 2202, 3040, 3092
AclWI GGATC 6 cut(s) 226, 683, 1023, 2204, 2699, 3167
AcoI YGGCCR 3 cut(s) 2022, 2397, 2547
AcsI RAATTY 4 cut(s) 83, 224, 1450, 2942
AcuI CTGAAG 5 cut(s) 1233, 1906, 2253, 2358, 3127
AcyI GRCGYC 2 cut(s) 532, 555
AfaI GTAC 4 cut(s) 586, 971, 1524, 2972
AfeI AGCGCT 1 cut(s) 1371
AfiI CCNNNNNNNGG 6 cut(s) 109, 527, 1539, 2766, 2854, 2891
AflII CTTAAG 1 cut(s) 1715
AflIII ACRYGT 1 cut(s) 1623
AgeI ACCGGT 2 cut(s) 731, 2759
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 4 cut(s) 329, 1126, 2561, 2916
AluBI AGCT 9 cut(s) 768, 1245, 1345, 1423, 1968, 2298, 2491, 2568, 2665
AluI AGCT 9 cut(s) 768, 1245, 1345, 1423, 1968, 2298, 2491, 2568, 2665
Alw21I GWGCWC 4 cut(s) 778, 954, 2570, 2952
Alw26I GTCTC 1 cut(s) 1217
AlwI GGATC 6 cut(s) 226, 683, 1023, 2204, 2699, 3167
AlwNI CAGNNNCTG 1 cut(s) 420
Aor51HI AGCGCT 1 cut(s) 1371
AoxI GGCC 6 cut(s) 2022, 2283, 2397, 2547, 2782, 3126
ApoI RAATTY 4 cut(s) 83, 224, 1450, 2942
AseI ATTAAT 3 cut(s) 48, 1728, 2169
AsiGI ACCGGT 2 cut(s) 731, 2759
Asp700I GAANNNNTTC 1 cut(s) 2299
AspLEI GCGC 6 cut(s) 205, 346, 534, 1126, 1372, 2280
AspS9I GGNCC 1 cut(s) 2600
AsuC2I CCSGG 1 cut(s) 394
AsuHPI GGTGA 8 cut(s) 364, 382, 1879, 1963, 2098, 2191, 3172, 3202
AvaII GGWCC 1 cut(s) 2600
AxyI CCTNAGG 1 cut(s) 560
BaeGI GKGCMC 1 cut(s) 1029
BaeI ACNNNNGTAYC 2 cut(s) 794, 827
BalI TGGCCA 2 cut(s) 2024, 2549
BanI GGYRCC 1 cut(s) 531
BanII GRGCYC 2 cut(s) 2570, 2988
BauI CACGAG 1 cut(s) 62
BbsI GAAGAC 1 cut(s) 3162
Bbv12I GWGCWC 4 cut(s) 778, 954, 2570, 2952
BbvCI CCTCAGC 1 cut(s) 1419
BceAI ACGGC 1 cut(s) 2797
BciT130I CCWGG 4 cut(s) 331, 1128, 2563, 2918
BciVI GTATCC 2 cut(s) 1391, 2851
BclI TGATCA 1 cut(s) 2103
BcnI CCSGG 1 cut(s) 394
BcoDI GTCTC 1 cut(s) 1217
BfaI CTAG 4 cut(s) 1748, 2154, 2828, 2966
BfoI RGCGCY 3 cut(s) 347, 535, 1373
BfrI CTTAAG 1 cut(s) 1715
BfuAI ACCTGC 1 cut(s) 1534
BfuI GTATCC 2 cut(s) 1391, 2851
BglII AGATCT 2 cut(s) 919, 3202
BmcAI AGTACT 1 cut(s) 586
Bme1390I CCNGG 5 cut(s) 331, 394, 1128, 2563, 2918
Bme18I GGWCC 1 cut(s) 2600
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 2600
BmiI GGNNCC 5 cut(s) 533, 579, 2151, 2601, 2729
BmrFI CCNGG 5 cut(s) 331, 394, 1128, 2563, 2918
BmrI ACTGGG 1 cut(s) 576
BmsI GCATC 9 cut(s) 266, 1494, 1944, 2034, 2178, 2659, 2688, 3052, 3151
BmuI ACTGGG 1 cut(s) 576
BpiI GAAGAC 1 cut(s) 3162
BpmI CTGGAG 5 cut(s) 532, 762, 1130, 1923, 2584
Bpu10I CCTNAGC 2 cut(s) 1419, 2192
BpuMI CCSGG 1 cut(s) 394
BsaAI YACGTR 1 cut(s) 300
BsaHI GRCGYC 2 cut(s) 532, 555
BsaJI CCNNGG 8 cut(s) 329, 330, 608, 1127, 2304, 2400, 2603, 2917
BsaWI WCCGGW 2 cut(s) 731, 2759
BsaXI ACNNNNNCTCC 4 cut(s) 1103, 1133, 1634, 1664
Bsc4I CCNNNNNNNGG 6 cut(s) 109, 527, 1539, 2766, 2854, 2891
Bse118I RCCGGY 3 cut(s) 731, 2242, 2759
Bse1I ACTGG 7 cut(s) 153, 582, 1113, 2035, 2213, 2296, 2481
Bse21I CCTNAGG 1 cut(s) 560
Bse3DI GCAATG 1 cut(s) 681
BseBI CCWGG 4 cut(s) 331, 1128, 2563, 2918
BseDI CCNNGG 8 cut(s) 329, 330, 608, 1127, 2304, 2400, 2603, 2917
BseLI CCNNNNNNNGG 6 cut(s) 109, 527, 1539, 2766, 2854, 2891
BseMI GCAATG 1 cut(s) 681
BseMII CTCAG 6 cut(s) 574, 1433, 1776, 2206, 2526, 2919
BseNI ACTGG 7 cut(s) 153, 582, 1113, 2035, 2213, 2296, 2481
BseRI GAGGAG 1 cut(s) 1655
BseSI GKGCMC 1 cut(s) 1029
BseYI CCCAGC 1 cut(s) 1022
BsgI GTGCAG 3 cut(s) 1513, 1984, 2740
Bsh1236I CGCG 2 cut(s) 203, 205
Bsh1285I CGRYCG 1 cut(s) 663
BshFI GGCC 6 cut(s) 2024, 2285, 2399, 2549, 2784, 3128
BshNI GGYRCC 1 cut(s) 531
BshTI ACCGGT 2 cut(s) 731, 2759
BsiEI CGRYCG 1 cut(s) 663
BsiHKAI GWGCWC 4 cut(s) 778, 954, 2570, 2952
BsiSI CCGG 5 cut(s) 129, 394, 732, 2243, 2760
BslFI GGGAC 2 cut(s) 860, 2518
BslI CCNNNNNNNGG 6 cut(s) 109, 527, 1539, 2766, 2854, 2891
BsmAI GTCTC 1 cut(s) 1217
BsmFI GGGAC 2 cut(s) 860, 2518
BsmI GAATGC 1 cut(s) 2618
BsnI GGCC 6 cut(s) 2024, 2285, 2399, 2549, 2784, 3128
Bsp1286I GDGCHC 6 cut(s) 778, 954, 1029, 2570, 2952, 2988
Bsp1407I TGTACA 1 cut(s) 2970
Bsp19I CCATGG 1 cut(s) 608
BspACI CCGC 6 cut(s) 205, 663, 692, 2202, 3040, 3092
BspANI GGCC 6 cut(s) 2024, 2285, 2399, 2549, 2784, 3128
BspCNI CTCAG 6 cut(s) 573, 1432, 1777, 2205, 2525, 2920
BspFNI CGCG 2 cut(s) 203, 205
BspHI TCATGA 1 cut(s) 2058
BspLI GGNNCC 5 cut(s) 533, 579, 2151, 2601, 2729
BspMI ACCTGC 1 cut(s) 1534
BspPI GGATC 6 cut(s) 226, 683, 1023, 2204, 2699, 3167
BspQI GCTCTTC 1 cut(s) 2305
BspT107I GGYRCC 1 cut(s) 531
BspTI CTTAAG 1 cut(s) 1715
BsrBI CCGCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 681
BsrFI RCCGGY 3 cut(s) 731, 2242, 2759
BsrGI TGTACA 1 cut(s) 2970
BsrI ACTGG 7 cut(s) 153, 582, 1113, 2035, 2213, 2296, 2481
BssAI RCCGGY 3 cut(s) 731, 2242, 2759
BssECI CCNNGG 8 cut(s) 329, 330, 608, 1127, 2304, 2400, 2603, 2917
BssNI GRCGYC 2 cut(s) 532, 555
BssSI CACGAG 1 cut(s) 62
BssT1I CCWWGG 2 cut(s) 608, 2603
Bst2BI CACGAG 1 cut(s) 62
Bst2UI CCWGG 4 cut(s) 331, 1128, 2563, 2918
Bst4CI ACNGT 5 cut(s) 431, 487, 931, 1183, 2545
Bst6I CTCTTC 2 cut(s) 1209, 2305
BstACI GRCGYC 2 cut(s) 532, 555
BstAFI CTTAAG 1 cut(s) 1715
BstAPI GCANNNNNTGC 2 cut(s) 214, 1757
BstAUI TGTACA 1 cut(s) 2970
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 9 cut(s) 518, 522, 1710, 1771, 1959, 1963, 2011, 2148, 2914
BstDEI CTNAG 6 cut(s) 560, 1419, 1785, 2192, 2512, 2928
BstDSI CCRYGG 1 cut(s) 608
BstFNI CGCG 2 cut(s) 203, 205
BstH2I RGCGCY 3 cut(s) 347, 535, 1373
BstHHI GCGC 6 cut(s) 205, 346, 534, 1126, 1372, 2280
BstMAI GTCTC 1 cut(s) 1217
BstMCI CGRYCG 1 cut(s) 663
BstNI CCWGG 4 cut(s) 331, 1128, 2563, 2918
BstNSI RCATGY 2 cut(s) 1627, 2013
BstSCI CCNGG 5 cut(s) 329, 392, 1126, 2561, 2916
BstSLI GKGCMC 1 cut(s) 1029
BstUI CGCG 2 cut(s) 203, 205
BstV2I GAAGAC 1 cut(s) 3162
BstX2I RGATCY 4 cut(s) 231, 919, 3172, 3202
BstYI RGATCY 4 cut(s) 231, 919, 3172, 3202
Bsu36I CCTNAGG 1 cut(s) 560
BsuI GTATCC 2 cut(s) 1391, 2851
BsuRI GGCC 6 cut(s) 2024, 2285, 2399, 2549, 2784, 3128
BtgI CCRYGG 1 cut(s) 608
BtrI CACGTC 1 cut(s) 352
BtsI GCAGTG 4 cut(s) 212, 1362, 2367, 2729
BtsIMutI CAGTG 8 cut(s) 160, 180, 212, 1106, 1362, 2367, 2550, 2729
BveI ACCTGC 1 cut(s) 1534
Cac8I GCNNGC 9 cut(s) 518, 522, 1710, 1771, 1959, 1963, 2011, 2148, 2914
CaiI CAGNNNCTG 1 cut(s) 420
CciI TCATGA 1 cut(s) 2058
CfoI GCGC 6 cut(s) 205, 346, 534, 1126, 1372, 2280
Cfr10I RCCGGY 3 cut(s) 731, 2242, 2759
Cfr13I GGNCC 1 cut(s) 2600
CseI GACGC 3 cut(s) 563, 645, 868
Csp6I GTAC 4 cut(s) 585, 970, 1523, 2971
CspAI ACCGGT 2 cut(s) 731, 2759
CviQI GTAC 4 cut(s) 585, 970, 1523, 2971
DdeI CTNAG 6 cut(s) 560, 1419, 1785, 2192, 2512, 2928
DinI GGCGCC 1 cut(s) 533
DraI TTTAAA 3 cut(s) 88, 676, 1797
EaeI YGGCCR 3 cut(s) 2022, 2397, 2547
Eam1104I CTCTTC 2 cut(s) 1209, 2305
EarI CTCTTC 2 cut(s) 1209, 2305
EciI GGCGGA 1 cut(s) 681
Ecl136II GAGCTC 1 cut(s) 2568
Eco130I CCWWGG 2 cut(s) 608, 2603
Eco24I GRGCYC 2 cut(s) 2570, 2988
Eco32I GATATC 1 cut(s) 1833
Eco47I GGWCC 1 cut(s) 2600
Eco47III AGCGCT 1 cut(s) 1371
Eco53kI GAGCTC 1 cut(s) 2568
Eco57I CTGAAG 5 cut(s) 1233, 1906, 2253, 2358, 3127
Eco81I CCTNAGG 1 cut(s) 560
EcoICRI GAGCTC 1 cut(s) 2568
EcoO109I RGGNCCY 1 cut(s) 2600
EcoRII CCWGG 4 cut(s) 329, 1126, 2561, 2916
EcoRV GATATC 1 cut(s) 1833
EcoT14I CCWWGG 2 cut(s) 608, 2603
EcoT22I ATGCAT 1 cut(s) 2176
EcoT38I GRGCYC 2 cut(s) 2570, 2988
EgeI GGCGCC 1 cut(s) 533
EheI GGCGCC 1 cut(s) 533
ErhI CCWWGG 2 cut(s) 608, 2603
FaqI GGGAC 2 cut(s) 860, 2518
FauI CCCGC 1 cut(s) 3085
FbaI TGATCA 1 cut(s) 2103
FblI GTMKAC 1 cut(s) 659
FriOI GRGCYC 2 cut(s) 2570, 2988
FspBI CTAG 4 cut(s) 1748, 2154, 2828, 2966
FspI TGCGCA 1 cut(s) 2279
GlaI GCGC 6 cut(s) 204, 345, 533, 1125, 1371, 2279
GsaI CCCAGC 1 cut(s) 1026
GsuI CTGGAG 5 cut(s) 532, 762, 1130, 1923, 2584
HaeII RGCGCY 3 cut(s) 347, 535, 1373
HaeIII GGCC 6 cut(s) 2024, 2285, 2399, 2549, 2784, 3128
HapII CCGG 5 cut(s) 129, 394, 732, 2243, 2760
HgaI GACGC 3 cut(s) 563, 645, 868
HhaI GCGC 6 cut(s) 205, 346, 534, 1126, 1372, 2280
Hin1I GRCGYC 2 cut(s) 532, 555
Hin6I GCGC 6 cut(s) 203, 344, 532, 1124, 1370, 2278
HinP1I GCGC 6 cut(s) 203, 344, 532, 1124, 1370, 2278
HincII GTYRAC 2 cut(s) 490, 660
HindII GTYRAC 2 cut(s) 490, 660
HinfI GANTC 8 cut(s) 65, 185, 325, 496, 599, 1043, 1252, 1907
HpaII CCGG 5 cut(s) 129, 394, 732, 2243, 2760
HphI GGTGA 8 cut(s) 364, 382, 1879, 1963, 2098, 2191, 3172, 3202
Hpy166II GTNNAC 7 cut(s) 490, 660, 934, 1298, 2971, 3180, 3210
Hpy188III TCNNGA 9 cut(s) 229, 461, 596, 635, 923, 1902, 1975, 2059, 2194
Hpy8I GTNNAC 7 cut(s) 490, 660, 934, 1298, 2971, 3180, 3210
Hpy99I CGWCG 5 cut(s) 356, 359, 431, 557, 661
HpyCH4III ACNGT 5 cut(s) 431, 487, 931, 1183, 2545
HpyCH4IV ACGT 3 cut(s) 299, 351, 1083
HpyF3I CTNAG 6 cut(s) 560, 1419, 1785, 2192, 2512, 2928
HpySE526I ACGT 3 cut(s) 299, 351, 1083
Hsp92I GRCGYC 2 cut(s) 532, 555
HspAI GCGC 6 cut(s) 203, 344, 532, 1124, 1370, 2278
KasI GGCGCC 1 cut(s) 531
Ksp22I TGATCA 1 cut(s) 2103
LguI GCTCTTC 1 cut(s) 2305
LweI GCATC 9 cut(s) 266, 1494, 1944, 2034, 2178, 2659, 2688, 3052, 3151
MaeI CTAG 4 cut(s) 1748, 2154, 2828, 2966
MaeII ACGT 3 cut(s) 299, 351, 1083
MaeIII GTNAC 7 cut(s) 1357, 1650, 1674, 2381, 2716, 3004, 3025
MbiI CCGCTC 1 cut(s) 665
MfeI CAATTG 1 cut(s) 2465
MflI RGATCY 4 cut(s) 231, 919, 3172, 3202
MhlI GDGCHC 6 cut(s) 778, 954, 1029, 2570, 2952, 2988
MlsI TGGCCA 2 cut(s) 2024, 2549
MluNI TGGCCA 2 cut(s) 2024, 2549
Mly113I GGCGCC 1 cut(s) 532
MlyI GAGTC 1 cut(s) 74
MmeI TCCRAC 6 cut(s) 450, 1047, 1420, 1473, 2113, 2669
Mox20I TGGCCA 2 cut(s) 2024, 2549
Mph1103I ATGCAT 1 cut(s) 2176
MroXI GAANNNNTTC 1 cut(s) 2299
MscI TGGCCA 2 cut(s) 2024, 2549
MslI CAYNNNNRTG 1 cut(s) 2769
Msp20I TGGCCA 2 cut(s) 2024, 2549
MspCI CTTAAG 1 cut(s) 1715
MspI CCGG 5 cut(s) 129, 394, 732, 2243, 2760
MspR9I CCNGG 5 cut(s) 331, 394, 1128, 2563, 2918
MunI CAATTG 1 cut(s) 2465
Mva1269I GAATGC 1 cut(s) 2618
MvaI CCWGG 4 cut(s) 331, 1128, 2563, 2918
MvnI CGCG 2 cut(s) 203, 205
NarI GGCGCC 1 cut(s) 532
NciI CCSGG 1 cut(s) 394
NcoI CCATGG 1 cut(s) 608
NlaIV GGNNCC 5 cut(s) 533, 579, 2151, 2601, 2729
NmeAIII GCCGAG 1 cut(s) 2425
NmuCI GTSAC 4 cut(s) 1357, 1650, 1674, 3004
NsbI TGCGCA 1 cut(s) 2279
NsiI ATGCAT 1 cut(s) 2176
NspI RCATGY 2 cut(s) 1627, 2013
PaeI GCATGC 1 cut(s) 2013
PagI TCATGA 1 cut(s) 2058
PasI CCCWGGG 1 cut(s) 330
PciI ACATGT 1 cut(s) 1623
PciSI GCTCTTC 1 cut(s) 2305
PctI GAATGC 1 cut(s) 2618
PdmI GAANNNNTTC 1 cut(s) 2299
PfeI GAWTC 7 cut(s) 185, 325, 496, 599, 1043, 1252, 1907
PflMI CCANNNNNTGG 2 cut(s) 1539, 2891
PinAI ACCGGT 2 cut(s) 731, 2759
PleI GAGTC 1 cut(s) 73
PluTI GGCGCC 1 cut(s) 535
PpsI GAGTC 1 cut(s) 73
Ppu21I YACGTR 1 cut(s) 300
PpuMI RGGWCCY 1 cut(s) 2600
PscI ACATGT 1 cut(s) 1623
PshBI ATTAAT 3 cut(s) 48, 1728, 2169
Psp124BI GAGCTC 1 cut(s) 2570
Psp5II RGGWCCY 1 cut(s) 2600
Psp6I CCWGG 4 cut(s) 329, 1126, 2561, 2916
PspFI CCCAGC 1 cut(s) 1022
PspGI CCWGG 4 cut(s) 329, 1126, 2561, 2916
PspN4I GGNNCC 5 cut(s) 533, 579, 2151, 2601, 2729
PspPI GGNCC 1 cut(s) 2600
PspPPI RGGWCCY 1 cut(s) 2600
PstNI CAGNNNCTG 1 cut(s) 420
PsuI RGATCY 4 cut(s) 231, 919, 3172, 3202
RsaI GTAC 4 cut(s) 586, 971, 1524, 2972
RsaNI GTAC 4 cut(s) 585, 970, 1523, 2971
RseI CAYNNNNRTG 1 cut(s) 2769
SacI GAGCTC 1 cut(s) 2570
SalI GTCGAC 1 cut(s) 658
SapI GCTCTTC 1 cut(s) 2305
Sau96I GGNCC 1 cut(s) 2600
ScaI AGTACT 1 cut(s) 586
SchI GAGTC 1 cut(s) 74
ScrFI CCNGG 5 cut(s) 331, 394, 1128, 2563, 2918
SduI GDGCHC 6 cut(s) 778, 954, 1029, 2570, 2952, 2988
SfaNI GCATC 9 cut(s) 266, 1494, 1944, 2034, 2178, 2659, 2688, 3052, 3151
SfoI GGCGCC 1 cut(s) 533
SinI GGWCC 1 cut(s) 2600
SmiMI CAYNNNNRTG 1 cut(s) 2769
SmlI CTYRAG 1 cut(s) 1715
SmoI CTYRAG 1 cut(s) 1715
SphI GCATGC 1 cut(s) 2013
SsiI CCGC 6 cut(s) 205, 663, 692, 2202, 3040, 3092
SspDI GGCGCC 1 cut(s) 531
SspI AATATT 3 cut(s) 1321, 2224, 2889
SspMI CTAG 4 cut(s) 1748, 2154, 2828, 2966
SstI GAGCTC 1 cut(s) 2570
StyD4I CCNGG 5 cut(s) 329, 392, 1126, 2561, 2916
StyI CCWWGG 2 cut(s) 608, 2603
TaaI ACNGT 5 cut(s) 431, 487, 931, 1183, 2545
TaiI ACGT 3 cut(s) 302, 354, 1086
TaqI TCGA 5 cut(s) 552, 659, 1976, 2814, 3084
TaqII GACCGA 1 cut(s) 2992
TatI WGTACW 3 cut(s) 584, 1522, 2970
TauI GCSGC 2 cut(s) 208, 3043
TfiI GAWTC 7 cut(s) 185, 325, 496, 599, 1043, 1252, 1907
TscAI CASTG 8 cut(s) 160, 187, 219, 1113, 1362, 2367, 2550, 2729
TseFI GTSAC 4 cut(s) 1357, 1650, 1674, 3004
Tsp45I GTSAC 4 cut(s) 1357, 1650, 1674, 3004
TspDTI ATGAA 8 cut(s) 1278, 1566, 1614, 1713, 1887, 2075, 2172, 2812
TspGWI ACGGA 4 cut(s) 58, 413, 445, 3165
TspRI CASTG 8 cut(s) 160, 187, 219, 1113, 1362, 2367, 2550, 2729
Van91I CCANNNNNTGG 2 cut(s) 1539, 2891
Vha464I CTTAAG 1 cut(s) 1715
VpaK11BI GGWCC 1 cut(s) 2600
VspI ATTAAT 3 cut(s) 48, 1728, 2169
XapI RAATTY 4 cut(s) 83, 224, 1450, 2942
XceI RCATGY 2 cut(s) 1627, 2013
XmiI GTMKAC 1 cut(s) 659
XmnI GAANNNNTTC 1 cut(s) 2299
XspI CTAG 4 cut(s) 1748, 2154, 2828, 2966
ZrmI AGTACT 1 cut(s) 586
Zsp2I ATGCAT 1 cut(s) 2176
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.