pycom13g21500

Cofactor assembly of complex C subunit B, CCB2/CCB4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr13
OG0011398
Physical Location & Seq
Reverse (-)
18236967 .. 18238967
2001 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1101 bp
ATGAATTTATATGTACTTGTTGTGTCTTCATATCGCGTGTCGCAGTATTCGTGTCCACCAGTGCAACTTAGGGTTCCACAACCAAGGAGTACCCAAAATTCCAAAATTGGGCCTAATCTCAACTTGGACCAGTGGACTGTCTCTTCAAAGTCAGCATTTCATCCCTCCACTCATTCCCACTTTTTTCTCAGCTCCCAAGCCACCCGTACAAGTAGAAAAAGTTCCTCAGAAAATATGACTAACACCAGCTCCATTCTTTCCCCAAATCCGTTAATTCGGTTGAAAATCCAAATCCCACCCAAATTTCGCGCCAGAAAGTTCCCCACAAATTTCTTACCAGTCTCCGCTCGTCTCGACGATTCCAAAAGCACCTCCACGGACCCCCAACTCAACCTCTCCGTGCTCCGCTTCACACTCGGAATACCTGGGCTGGACGAATCCTACTTACCAAGATGGATTGGATATGGGTTCGGTTCGCTTTTGATTTTGAACCATTTTGTTGGTTCCACTGCAACCCCAGCACAGCTTAGGACGGAGGCTTTGGGTCTGTCTTTGGCTGCATTCTCTGTCGCACTTCCCTATCTTGGAAAATTTCTCAAGGGTGCTACTCCGATGGATCAGACGACCATCCCACAAGGGTGTGAGCAAATATTTGTTATATCACAAAATGTCTCAGATACTCAGAAGGAGGACTTGGCTTGGGCTACATACATAGTGTTGCGCAATACAAACACCATAGCAGTGATGATATCTGTACAAAATGAACTGTGCGTACGCGGCTATTGGAACATACCAGATGAGGTTTCAAAAGCCAACGTACTTGGATGGCTTGAGAAACAGATTGAAAGTATTGGCATTTCTGATTTGAAGGAGACCCTGTACCTATCTCAGATTGAAGATTCTGGACTTTGGGAGATGCTACCGCAGGGGACTCGTTCTCTCCTTGTACAACCGATTCTCCAAGTTGAGCATTCAATTGACAATGTGATCCAGAGAGTTGAGGGATTTGTCTTGTTGGCTTCCACCATGAGGTATGCATATAGTGATAAGGACAGAGCCTGGATTGGTGCTCTTACAAACAAGTTCAGAGGTAAGCAATAA

Protein Analysis

367

Amino Acids

40.87

Weight (kDa)

9.25

Isoelectric Point (pI)

35.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCB2_CCB4 PF11152 149 - 362 1.5e-54 Cofactor assembly of complex C subunit B, CCB2/CCB4
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011398)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G52110 AT5G52110
fragaria_vesca FvH4_7g15530
malus_domestica MD13G1243600.v1.1
prunus_persica Prupe.1G077000_v2.0.a1 Prupe.1G077000_v2.0.a1
pyrus_communis pycom13g21500
rosa_chinensis RchiOBHm_Chr1g0376741
rosa_laevigata RLG00000026574
rosa_multiflora Rmu_sc0003609.1_g000004
rosa_roxburghii Rroxscaffold_4G00281780 Rroxscaffold_4G00281830
rosa_rugosa Rorug01G0397900 Rorug01G0398000
rosa_samantha Rh1AG415300 Rh1BG374900 Rh1CG388700 Rh1DG405500
rosa_wichuraiana Rw0G004570 Rw1G036410

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 722
AccBSI CCGCTC 1 cut(s) 347
AccII CGCG 3 cut(s) 36, 309, 777
AciI CCGC 4 cut(s) 345, 406, 777, 923
AclWI GGATC 2 cut(s) 624, 982
AcsI RAATTY 5 cut(s) 4, 97, 302, 328, 590
AfaI GTAC 8 cut(s) 15, 91, 208, 756, 774, 819, 881, 948
AfiI CCNNNNNNNGG 3 cut(s) 108, 584, 1029
AgsI TTSAA 8 cut(s) 147, 283, 490, 807, 845, 868, 896, 975
AjnI CCWGG 2 cut(s) 424, 1058
AjuI GAANNNNNNNTTGG 2 cut(s) 677, 709
AleI CACNNNNGTG 1 cut(s) 637
AluBI AGCT 3 cut(s) 192, 249, 526
AluI AGCT 3 cut(s) 192, 249, 526
Alw21I GWGCWC 2 cut(s) 405, 1072
Alw26I GTCTC 5 cut(s) 145, 346, 356, 676, 866
AlwI GGATC 2 cut(s) 624, 982
AlwNI CAGNNNCTG 1 cut(s) 1059
AoxI GGCC 1 cut(s) 110
ApeKI GCWGC 1 cut(s) 557
ApoI RAATTY 5 cut(s) 4, 97, 302, 328, 590
ArsI GACNNNNNNTTYG 2 cut(s) 523, 555
Asp700I GAANNNNTTC 1 cut(s) 220
AspLEI GCGC 2 cut(s) 311, 723
AspS9I GGNCC 3 cut(s) 110, 127, 379
AvaII GGWCC 2 cut(s) 127, 379
BbsI GAAGAC 1 cut(s) 18
Bbv12I GWGCWC 2 cut(s) 405, 1072
BbvI GCAGC 1 cut(s) 544
BccI CCATC 4 cut(s) 447, 607, 635, 819
BcgI CGANNNNNNTGC 2 cut(s) 914, 948
BciT130I CCWGG 2 cut(s) 426, 1060
BcoDI GTCTC 5 cut(s) 145, 346, 356, 676, 866
BisI GCNGC 2 cut(s) 558, 778
BlsI GCNGC 2 cut(s) 559, 779
Bme1390I CCNGG 2 cut(s) 426, 1060
Bme18I GGWCC 2 cut(s) 127, 379
BmgT120I GGNCC 3 cut(s) 110, 127, 379
BmiI GGNNCC 3 cut(s) 75, 381, 505
BmrFI CCNGG 2 cut(s) 426, 1060
BmsI GCATC 1 cut(s) 906
BpiI GAAGAC 1 cut(s) 18
Bpu10I CCTNAGC 1 cut(s) 527
BpuEI CTTGAG 2 cut(s) 581, 851
BsaI GGTCTC 1 cut(s) 866
BsaJI CCNNGG 3 cut(s) 83, 375, 425
BsaXI ACNNNNNCTCC 6 cut(s) 233, 263, 863, 893, 942, 972
Bsc4I CCNNNNNNNGG 3 cut(s) 108, 584, 1029
Bse1I ACTGG 3 cut(s) 59, 130, 338
BseBI CCWGG 2 cut(s) 426, 1060
BseDI CCNNGG 3 cut(s) 83, 375, 425
BseGI GGATG 3 cut(s) 160, 627, 830
BseLI CCNNNNNNNGG 3 cut(s) 108, 584, 1029
BseMII CTCAG 5 cut(s) 202, 240, 687, 695, 902
BseNI ACTGG 3 cut(s) 59, 130, 338
BseXI GCAGC 1 cut(s) 544
BseYI CCCAGC 1 cut(s) 517
Bsh1236I CGCG 3 cut(s) 36, 309, 777
BshFI GGCC 1 cut(s) 112
BsiHKAI GWGCWC 2 cut(s) 405, 1072
BsiWI CGTACG 1 cut(s) 772
BslFI GGGAC 1 cut(s) 943
BslI CCNNNNNNNGG 3 cut(s) 108, 584, 1029
BsmAI GTCTC 5 cut(s) 145, 346, 356, 676, 866
BsmBI CGTCTC 1 cut(s) 356
BsmFI GGGAC 1 cut(s) 943
BsmI GAATGC 2 cut(s) 560, 970
BsnI GGCC 1 cut(s) 112
Bso31I GGTCTC 1 cut(s) 866
Bsp1286I GDGCHC 2 cut(s) 405, 1072
Bsp1407I TGTACA 2 cut(s) 754, 946
Bsp143I GATC 2 cut(s) 616, 987
BspACI CCGC 4 cut(s) 345, 406, 777, 923
BspANI GGCC 1 cut(s) 112
BspCNI CTCAG 5 cut(s) 201, 239, 686, 694, 901
BspFNI CGCG 3 cut(s) 36, 309, 777
BspLI GGNNCC 3 cut(s) 75, 381, 505
BspPI GGATC 2 cut(s) 624, 982
BspTNI GGTCTC 1 cut(s) 866
BsrBI CCGCTC 1 cut(s) 347
BsrGI TGTACA 2 cut(s) 754, 946
BsrI ACTGG 3 cut(s) 59, 130, 338
BssECI CCNNGG 3 cut(s) 83, 375, 425
BssMI GATC 2 cut(s) 616, 987
BssT1I CCWWGG 1 cut(s) 83
Bst2UI CCWGG 2 cut(s) 426, 1060
Bst4CI ACNGT 2 cut(s) 139, 768
Bst6I CTCTTC 1 cut(s) 148
BstAUI TGTACA 2 cut(s) 754, 946
BstDEI CTNAG 7 cut(s) 68, 188, 226, 527, 673, 681, 888
BstDSI CCRYGG 1 cut(s) 375
BstF5I GGATG 3 cut(s) 160, 627, 830
BstFNI CGCG 3 cut(s) 36, 309, 777
BstHHI GCGC 2 cut(s) 311, 723
BstKTI GATC 2 cut(s) 619, 990
BstMAI GTCTC 5 cut(s) 145, 346, 356, 676, 866
BstMBI GATC 2 cut(s) 616, 987
BstMWI GCNNNNNNNGC 2 cut(s) 518, 777
BstNI CCWGG 2 cut(s) 426, 1060
BstSCI CCNGG 2 cut(s) 424, 1058
BstUI CGCG 3 cut(s) 36, 309, 777
BstV1I GCAGC 1 cut(s) 544
BstV2I GAAGAC 1 cut(s) 18
BstXI CCANNNNNNTGG 1 cut(s) 500
BsuRI GGCC 1 cut(s) 112
BtgI CCRYGG 1 cut(s) 375
BtsCI GGATG 3 cut(s) 160, 627, 830
BtsI GCAGTG 2 cut(s) 507, 747
BtsIMutI CAGTG 4 cut(s) 66, 137, 507, 747
CaiI CAGNNNCTG 1 cut(s) 1059
CfoI GCGC 2 cut(s) 311, 723
Cfr13I GGNCC 3 cut(s) 110, 127, 379
Csp6I GTAC 8 cut(s) 14, 90, 207, 755, 773, 818, 880, 947
CviAII CATG 1 cut(s) 1027
CviQI GTAC 8 cut(s) 14, 90, 207, 755, 773, 818, 880, 947
DdeI CTNAG 7 cut(s) 68, 188, 226, 527, 673, 681, 888
DpnI GATC 2 cut(s) 618, 989
DpnII GATC 2 cut(s) 616, 987
Eam1104I CTCTTC 1 cut(s) 148
EarI CTCTTC 1 cut(s) 148
Eco130I CCWWGG 1 cut(s) 83
Eco31I GGTCTC 1 cut(s) 866
Eco32I GATATC 1 cut(s) 750
Eco47I GGWCC 2 cut(s) 127, 379
EcoRII CCWGG 2 cut(s) 424, 1058
EcoRV GATATC 1 cut(s) 750
EcoT14I CCWWGG 1 cut(s) 83
EcoT22I ATGCAT 1 cut(s) 1039
ErhI CCWWGG 1 cut(s) 83
Esp3I CGTCTC 1 cut(s) 356
FaeI CATG 1 cut(s) 1030
FalI AAGNNNNNCTT 2 cut(s) 677, 709
FaqI GGGAC 1 cut(s) 943
FatI CATG 1 cut(s) 1026
Fnu4HI GCNGC 2 cut(s) 558, 778
FokI GGATG 3 cut(s) 147, 614, 837
Fsp4HI GCNGC 2 cut(s) 558, 778
FspI TGCGCA 1 cut(s) 722
GlaI GCGC 2 cut(s) 310, 722
GluI GCNGC 2 cut(s) 558, 778
GsaI CCCAGC 1 cut(s) 521
HaeIII GGCC 1 cut(s) 112
HhaI GCGC 2 cut(s) 311, 723
Hin1II CATG 1 cut(s) 1030
Hin6I GCGC 2 cut(s) 309, 721
HinP1I GCGC 2 cut(s) 309, 721
HinfI GANTC 5 cut(s) 359, 437, 899, 931, 955
Hpy166II GTNNAC 2 cut(s) 56, 135
Hpy188I TCNGA 9 cut(s) 229, 419, 612, 621, 676, 684, 862, 891, 1088
Hpy188III TCNNGA 3 cut(s) 353, 903, 991
Hpy8I GTNNAC 2 cut(s) 56, 135
Hpy99I CGWCG 1 cut(s) 359
HpyAV CCTTC 2 cut(s) 679, 862
HpyCH4III ACNGT 2 cut(s) 139, 768
HpyCH4IV ACGT 1 cut(s) 816
HpyCH4V TGCA 4 cut(s) 64, 512, 560, 1037
HpyF10VI GCNNNNNNNGC 2 cut(s) 518, 777
HpyF3I CTNAG 7 cut(s) 68, 188, 226, 527, 673, 681, 888
HpySE526I ACGT 1 cut(s) 816
Hsp92II CATG 1 cut(s) 1030
HspAI GCGC 2 cut(s) 309, 721
Kzo9I GATC 2 cut(s) 616, 987
LmnI GCTCC 3 cut(s) 197, 254, 408
Lsp1109I GCAGC 1 cut(s) 544
LweI GCATC 1 cut(s) 906
MaeII ACGT 1 cut(s) 816
MalI GATC 2 cut(s) 618, 989
MbiI CCGCTC 1 cut(s) 347
MboI GATC 2 cut(s) 616, 987
MboII GAAGA 3 cut(s) 18, 135, 908
MfeI CAATTG 1 cut(s) 975
MhlI GDGCHC 2 cut(s) 405, 1072
MluCI AATT 8 cut(s) 4, 97, 105, 273, 302, 328, 590, 975
MlyI GAGTC 1 cut(s) 925
Mph1103I ATGCAT 1 cut(s) 1039
MroXI GAANNNNTTC 1 cut(s) 220
MseI TTAA 1 cut(s) 272
MslI CAYNNNNRTG 2 cut(s) 637, 740
MspR9I CCNGG 2 cut(s) 426, 1060
MunI CAATTG 1 cut(s) 975
Mva1269I GAATGC 2 cut(s) 560, 970
MvaI CCWGG 2 cut(s) 426, 1060
MvnI CGCG 3 cut(s) 36, 309, 777
MwoI GCNNNNNNNGC 2 cut(s) 518, 777
NdeII GATC 2 cut(s) 616, 987
NlaIII CATG 1 cut(s) 1030
NlaIV GGNNCC 3 cut(s) 75, 381, 505
NsbI TGCGCA 1 cut(s) 722
NsiI ATGCAT 1 cut(s) 1039
OliI CACNNNNGTG 1 cut(s) 637
PcsI WCGNNNNNNNCGW 1 cut(s) 47
PctI GAATGC 2 cut(s) 560, 970
PdmI GAANNNNTTC 1 cut(s) 220
PfeI GAWTC 4 cut(s) 359, 437, 899, 955
Pfl23II CGTACG 1 cut(s) 772
PkrI GCNGC 2 cut(s) 559, 779
PleI GAGTC 1 cut(s) 925
PpsI GAGTC 1 cut(s) 925
Psp6I CCWGG 2 cut(s) 424, 1058
PspFI CCCAGC 1 cut(s) 517
PspGI CCWGG 2 cut(s) 424, 1058
PspLI CGTACG 1 cut(s) 772
PspN4I GGNNCC 3 cut(s) 75, 381, 505
PspPI GGNCC 3 cut(s) 110, 127, 379
PsrI GAACNNNNNNTAC 4 cut(s) 205, 237, 756, 788
PstNI CAGNNNCTG 1 cut(s) 1059
RsaI GTAC 8 cut(s) 15, 91, 208, 756, 774, 819, 881, 948
RsaNI GTAC 8 cut(s) 14, 90, 207, 755, 773, 818, 880, 947
RseI CAYNNNNRTG 2 cut(s) 637, 740
SaqAI TTAA 1 cut(s) 272
SatI GCNGC 2 cut(s) 558, 778
Sau3AI GATC 2 cut(s) 616, 987
Sau96I GGNCC 3 cut(s) 110, 127, 379
SchI GAGTC 1 cut(s) 925
ScrFI CCNGG 2 cut(s) 426, 1060
SduI GDGCHC 2 cut(s) 405, 1072
SfaNI GCATC 1 cut(s) 906
SinI GGWCC 2 cut(s) 127, 379
SmiMI CAYNNNNRTG 2 cut(s) 637, 740
SmlI CTYRAG 2 cut(s) 596, 830
SmoI CTYRAG 2 cut(s) 596, 830
Sse9I AATT 8 cut(s) 4, 97, 105, 273, 302, 328, 590, 975
SsiI CCGC 4 cut(s) 345, 406, 777, 923
SspI AATATT 1 cut(s) 651
StyD4I CCNGG 2 cut(s) 424, 1058
StyI CCWWGG 1 cut(s) 83
TaaI ACNGT 2 cut(s) 139, 768
TaiI ACGT 1 cut(s) 819
TaqI TCGA 1 cut(s) 354
TasI AATT 8 cut(s) 4, 97, 105, 273, 302, 328, 590, 975
TatI WGTACW 3 cut(s) 13, 754, 946
TauI GCSGC 1 cut(s) 780
TfiI GAWTC 4 cut(s) 359, 437, 899, 955
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
TscAI CASTG 4 cut(s) 66, 137, 514, 747
TseI GCWGC 1 cut(s) 557
TspDTI ATGAA 4 cut(s) 17, 18, 149, 777
TspGWI ACGGA 4 cut(s) 258, 388, 392, 548
TspRI CASTG 4 cut(s) 66, 137, 514, 747
VpaK11BI GGWCC 2 cut(s) 127, 379
XapI RAATTY 5 cut(s) 4, 97, 302, 328, 590
XmnI GAANNNNTTC 1 cut(s) 220
Zsp2I ATGCAT 1 cut(s) 1039
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.