pycom14g00310

Ankyrin repeats (many copies)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0004406
Physical Location & Seq
Reverse (-)
180688 .. 186762
6075 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 3084 bp
ATGGAAGCCTCACCTCAAGGACAAAAGCCTGCTCGCAAATTTCTGTCGACTGGAACCCAGAAAGATCTGTGCATTGTTGTGCGTGAAGGATCATTGGCTGATGTGGATTCAGCACTAGGTTTACTGAAGAAGAGTGGTGGTGATATAAATTCAAGAAATATTTTTGGTCTCACTCCTCTTCATATTGCAACATGGAGAAATCACCTTCCCATTGTCAGGACACTTCTTGCAGCTGGTGCTGACCCAGATGCTAGGGATGGGGAATCTGGATGGAGTAGCCTTCACAGAGCTCTGCATTTTGGTCATCTTGCAGTGGCAAGTATACTTCTCCTGTCTGGTGCTTCTATCACACTAGAGGATTCTAAATTTCGAACACCTATCGATCTCCTATCTGGGCCTGTCATGCAAGCTCTTCAAAATGGAGATAATTCTGTTACTACAGAGGTTTATAGTTGGGGAAGTGGTACAAACTATCAACTTGGAACTGGAAATGCGCACATACAAAAGTTACCATGCAAGGTTGATGTTCTTGGTGGTTCTTTAATCAAATTGGTTTCTGCTGCTAAGTTCCATAGCGTGGCAGTCACTTCTCGAGGAGAAGTCTACACTTGGGGATTTGGAAGAGGAGGACGACTTGGTCACCCTGATTTTGATATACACAGCGGTCAAGCTGCAGTTATCACACCGCGTCAGGTGACTTTTGGTATAGGGTCCCGTCGGGTGAAGGCAATTGCGGCAGCTAAACATCACACCGTTATTGCTACGGAGGGTGGGGAAGTGTTCACCTGGGGATCCAACAGAGAGGGTCAGCTTGGCTACACTTCTGTGGATACCCAACCTACTCCTCGTAGAGTGAATTATCTTAGGTCAAAAATTGTTGAAGTTGCTTCAGCCAACAAGCACACTGCTGTTGTTTCCGATGCCGGTGAAGTTTTCACATGGGGCTGCAATAGGGAGGGTCAGTTAGGCTATGGTACATCCAACTCTGCATCAAACTACACTCCAAGATTAGTTGAATCCTTGAAAGGCAAGGTTTTTAAGGGGGTTGCAGCAGCAAAATATCACACAATTGTTTTGGGAGTTGATGGGGAGGTATACACTTGGGGTCATCGAATTGTTACGCCAAAACGTGTTGTAATTGCTAGAAACTTGAAAAAGAGTGGAACTGCCACTTTGAAGTTTCATCGCAAAGAACGGCTTCATGTGGTCTCTATAGCTGCAGGGATGGTACATAGCACAGCCCTGACAGATGATGGCGCATTATTTTATTGGGTGTCATCAGATACGGACTTCAGATGCCAGCAGCTGTATTCATTGGGTGGAAGAAGTATGGTGAACATATCTGCAGGGAAATACTGGACTGCTGCAGTTACGGCTACAGGTGATGTTTACATGTGGGATGGGAAGAAAGGTAAGGATAAGCCACCGGTTGCAACTCGGTTACATGGTACAAAGCGGGCTAGTTCAGTTTCTGTAGGCGAAACACACTTGTTGATTATTGGCTCCCTTTATCACCCCGCCTTTACTTCCAATAACCCTCAGAAGCAGAAGTCAAATGTCAAAGATGAGCTAGAAGAGCTTGATGAAGATCTTATGTTCAATGACATGGAGTCTGATAATCTTTTACCTACTATCCAAAATGATGATGCTGGGATAAAGGCCATACCAAGCTTAAAAAGTCTTTGTGAAAAAGTGGCTGCAGATAATCTAGTGGAGCCACGAAATACTATTCAACTGCTTGAAATTGCCGATTCACTTGTGGCGGATGATTTGCGGAAGTACTGTGAGGAAATTGCCATACGCAACCTTGACTATATTTTTACGGTATCATCACAAGCTATTGCTAGTGCTTCACCATATGTTCTGGCTAACCTTGAAAATGTATTGGACCTTAGGTCATCTGAACCATGGAGTTACCGTCGTCTCCCTACTCCTACAGCTACTTTGCCTGCTATTATTTGTACTGAAGAGGAAGATAGTGAGACTGAGGTTCAAAGGACACGCGATGGCCATACAGAGCAATCCGTATGGAAAAGTAAAATCCATCAAAGGACAGAATCCTTTCTTCAACCCAAAGACGACTCAAATATTGAAAAGCAAGTTCGAGCCTTGTGGAAAAAGCTGCAGCAGATTGAGATGCTTGAAGCAAAACAGTCTAGGGGGCAAGTTCTTGATAACCAGCAAATTGCAAAGCTTGAAACAAGGTCATCTTTAGAGAGCTCGCTGGCGGAGCTTGGTGTTCCAGTTGAGGCACCACTGTTGAGGGCACCATCATCAGGTTTGGTAGATGGAAAAGGGAACAAAAAGGTTGAGCCATCAAAAAAACAGAGGAGAAGGAACAAAAAGATGGCAACACAAGTGGATATGGGGTCGTGTTTTTTAGGAAATGAAGTAGAACCAGACCATGAAAATGGTTTTTCGAATATCGAAATCTCTCAAACCTCAAAGAATACGGGGGATGTCATGATTGAAGAGATTGCAACAAGCCAATCCAATCAGGAGTCAGCTTTGTGTGTTCAGAAGGACAATTTCAACCTAACAAATAACAAGTGTTCTTCACCAGTAGTTTCCAAGAAGAAGAATAAAAAGGGTGGGCTTTCTATGTTCTTGAGTGGCGCTCTTGATGATGCCCCCAAGTTTGTTGCACCACCTCCACCATCTCCGAAAAGTGAAGGCCCTGCATGGGGTGGGGCTAAATTTTCCAAAGGTTTTGCATCCCTTCGCGAGATTCAGGATGAGCAAGGCAAAACCAAGGAGAGTCGGTCAACTAGGAACAAAAATCACGTGGAAGATCCTTCTGATGCGAGAAGCGATGGGAAGATTCGACTGAGTTCGTTTTTACCTTCCAAGCCAATACCATTGGTCTCAGCCCATACTTCACTGGCATCTGATGGAGAAAGAGGTACACCTCCTTGGACTGCGTCAGGAACTCCTCCCCTTGTTTCCCGGCCATCTCTTAGGGACATCCAAATGCAGCAGCAAGGAAAGCAACACCAGAGTGTTTCCCACAGTCCAAAGACAAAAACCGCCGGATTCTCAGTCACAAGTGGTCAGGGATCCGGCCATTGGATACTTCCGGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_4 Ank_5 RCC1

Protein Analysis

1028

Amino Acids

111.7

Weight (kDa)

8.95

Isoelectric Point (pI)

39.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 40 - 120 8.7e-12 Ankyrin repeats (3 copies)
Ank_5 PF13857 46 - 97 7.9e-08 Ankyrin repeats (many copies)
Ank PF00023 56 - 86 5.2e-08 Ankyrin repeat
Ank_4 PF13637 67 - 110 8e-08 Ankyrin repeats (many copies)
WD40_RLD PF25390 148 - 369 6.1e-50 RCC1-like domain
RCC1 PF00415 148 - 195 2e-09 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 199 - 253 2.5e-08 Regulator of chromosome condensation (RCC1) repeat
RCC1_2 PF13540 241 - 270 5e-06 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 258 - 305 3.1e-12 Regulator of chromosome condensation (RCC1) repeat
RCC1 PF00415 309 - 359 4.6e-13 Regulator of chromosome condensation (RCC1) repeat
WD40_RLD PF25390 398 - 500 2.7e-08 RCC1-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 636
Acc16I TGCGCA 1 cut(s) 495
AccB1I GGYRCC 2 cut(s) 2251, 2266
AccB7I CCANNNNNTGG 1 cut(s) 577
AccI GTMKAC 4 cut(s) 47, 322, 603, 1095
AccII CGCG 3 cut(s) 688, 2004, 2724
AccIII TCCGGA 1 cut(s) 3076
AciI CCGC 9 cut(s) 663, 686, 734, 1456, 1518, 1763, 1774, 2228, 3027
AclWI GGATC 6 cut(s) 97, 786, 799, 2786, 3051, 3064
AcoI YGGCCR 3 cut(s) 2008, 2948, 3061
AcsI RAATTY 4 cut(s) 38, 148, 365, 2696
AcuI CTGAAG 4 cut(s) 146, 873, 1276, 1986
AcvI CACGTG 1 cut(s) 2785
AfaI GTAC 7 cut(s) 466, 976, 1230, 1450, 1781, 1963, 2905
AfiI CCNNNNNNNGG 6 cut(s) 216, 577, 2276, 2683, 2684, 3066
AflIII ACRYGT 2 cut(s) 1129, 1392
AgeI ACCGGT 1 cut(s) 1426
AhdI GACNNNNNGTC 2 cut(s) 1609, 1894
AjnI CCWGG 1 cut(s) 785
AleI CACNNNNGTG 2 cut(s) 824, 2997
Alw21I GWGCWC 2 cut(s) 292, 2222
Alw26I GTCTC 5 cut(s) 173, 1213, 1928, 1976, 2869
AlwI GGATC 6 cut(s) 97, 786, 799, 2786, 3051, 3064
AlwNI CAGNNNCTG 2 cut(s) 1306, 1472
Ama87I CYCGRG 1 cut(s) 591
Aor13HI TCCGGA 1 cut(s) 3076
AoxI GGCC 6 cut(s) 395, 1659, 2008, 2674, 2948, 3061
ApoI RAATTY 4 cut(s) 38, 148, 365, 2696
AsiGI ACCGGT 1 cut(s) 1426
Asp700I GAANNNNTTC 2 cut(s) 1197, 2061
AspLEI GCGC 3 cut(s) 496, 1259, 2618
AspS9I GGNCC 4 cut(s) 395, 711, 1888, 2675
AsuC2I CCSGG 1 cut(s) 2947
AsuII TTCGAA 2 cut(s) 370, 2420
AvaI CYCGRG 1 cut(s) 591
AvaII GGWCC 2 cut(s) 711, 1888
AxyI CCTNAGG 1 cut(s) 1892
BaeGI GKGCMC 1 cut(s) 2269
BalI TGGCCA 1 cut(s) 2010
BamHI GGATCC 2 cut(s) 791, 3056
BanI GGYRCC 2 cut(s) 2251, 2266
BanII GRGCYC 2 cut(s) 292, 2222
BbrPI CACGTG 1 cut(s) 2785
Bbv12I GWGCWC 2 cut(s) 292, 2222
BceAI ACGGC 2 cut(s) 1211, 1389
BciT130I CCWGG 1 cut(s) 787
BciVI GTATCC 2 cut(s) 823, 3063
BcnI CCSGG 1 cut(s) 2947
BcoDI GTCTC 5 cut(s) 173, 1213, 1928, 1976, 2869
BfoI RGCGCY 1 cut(s) 2619
BfuI GTATCC 2 cut(s) 823, 3063
BglII AGATCT 2 cut(s) 64, 1588
BmcAI AGTACT 1 cut(s) 1781
Bme1390I CCNGG 2 cut(s) 787, 2947
Bme18I GGWCC 2 cut(s) 711, 1888
BmeRI GACNNNNNGTC 2 cut(s) 1609, 1894
BmeT110I CYCGRG 1 cut(s) 591
BmgT120I GGNCC 4 cut(s) 395, 711, 1888, 2675
BmiI GGNNCC 9 cut(s) 55, 712, 713, 793, 1504, 1716, 2253, 2268, 3058
BmrFI CCNGG 2 cut(s) 787, 2947
BplI GAGNNNNNCTC 2 cut(s) 2602, 2634
Bpu14I TTCGAA 2 cut(s) 370, 2420
BpuEI CTTGAG 1 cut(s) 2629
BpuMI CCSGG 1 cut(s) 2947
Bsa29I ATCGAT 1 cut(s) 381
BsaAI YACGTR 1 cut(s) 2785
BsaBI GATNNNNATC 1 cut(s) 1587
BsaI GGTCTC 3 cut(s) 173, 1213, 2869
BsaJI CCNNGG 4 cut(s) 786, 1907, 2751, 2912
BsaWI WCCGGW 2 cut(s) 1426, 3076
BsaXI ACNNNNNCTCC 6 cut(s) 947, 977, 1903, 1933, 2747, 2777
Bsc4I CCNNNNNNNGG 6 cut(s) 216, 577, 2276, 2683, 2684, 3066
Bse118I RCCGGY 2 cut(s) 923, 1426
Bse1I ACTGG 6 cut(s) 55, 490, 1361, 2243, 2561, 2886
Bse21I CCTNAGG 1 cut(s) 1892
Bse8I GATNNNNATC 1 cut(s) 1587
BseAI TCCGGA 1 cut(s) 3076
BseBI CCWGG 1 cut(s) 787
BseCI ATCGAT 1 cut(s) 381
BseDI CCNNGG 4 cut(s) 786, 1907, 2751, 2912
BseJI GATNNNNATC 1 cut(s) 1587
BseLI CCNNNNNNNGG 6 cut(s) 216, 577, 2276, 2683, 2684, 3066
BseMII CTCAG 5 cut(s) 1553, 1977, 2819, 2880, 3051
BseNI ACTGG 6 cut(s) 55, 490, 1361, 2243, 2561, 2886
BseRI GAGGAG 6 cut(s) 165, 609, 639, 834, 2344, 2922
BseSI GKGCMC 1 cut(s) 2269
BseYI CCCAGC 1 cut(s) 1649
Bsh1236I CGCG 3 cut(s) 688, 2004, 2724
BshFI GGCC 6 cut(s) 397, 1661, 2010, 2676, 2950, 3063
BshNI GGYRCC 2 cut(s) 2251, 2266
BshTI ACCGGT 1 cut(s) 1426
BshVI ATCGAT 1 cut(s) 381
BsiHKAI GWGCWC 2 cut(s) 292, 2222
BsiHKCI CYCGRG 1 cut(s) 591
BsiSI CCGG 6 cut(s) 924, 1427, 2947, 3030, 3060, 3077
BslFI GGGAC 2 cut(s) 697, 2975
BslI CCNNNNNNNGG 6 cut(s) 216, 577, 2276, 2683, 2684, 3066
BsmAI GTCTC 5 cut(s) 173, 1213, 1928, 1976, 2869
BsmBI CGTCTC 1 cut(s) 1928
BsmFI GGGAC 2 cut(s) 697, 2975
BsnI GGCC 6 cut(s) 397, 1661, 2010, 2676, 2950, 3063
Bso31I GGTCTC 3 cut(s) 173, 1213, 2869
BsoBI CYCGRG 1 cut(s) 591
Bsp119I TTCGAA 2 cut(s) 370, 2420
Bsp1286I GDGCHC 3 cut(s) 292, 2222, 2269
Bsp13I TCCGGA 1 cut(s) 3076
Bsp143I GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
Bsp19I CCATGG 1 cut(s) 1907
Bsp68I TCGCGA 1 cut(s) 2724
BspACI CCGC 9 cut(s) 663, 686, 734, 1456, 1518, 1763, 1774, 2228, 3027
BspANI GGCC 6 cut(s) 397, 1661, 2010, 2676, 2950, 3063
BspCNI CTCAG 5 cut(s) 1552, 1978, 2820, 2879, 3050
BspDI ATCGAT 1 cut(s) 381
BspEI TCCGGA 1 cut(s) 3076
BspFNI CGCG 3 cut(s) 688, 2004, 2724
BspHI TCATGA 1 cut(s) 2463
BspLI GGNNCC 9 cut(s) 55, 712, 713, 793, 1504, 1716, 2253, 2268, 3058
BspMAI CTGCAG 6 cut(s) 676, 1222, 1348, 1369, 1702, 2127
BspPI GGATC 6 cut(s) 97, 786, 799, 2786, 3051, 3064
BspQI GCTCTTC 2 cut(s) 417, 1569
BspT104I TTCGAA 2 cut(s) 370, 2420
BspT107I GGYRCC 2 cut(s) 2251, 2266
BspTNI GGTCTC 3 cut(s) 173, 1213, 2869
BsrFI RCCGGY 2 cut(s) 923, 1426
BsrI ACTGG 6 cut(s) 55, 490, 1361, 2243, 2561, 2886
BssAI RCCGGY 2 cut(s) 923, 1426
BssECI CCNNGG 4 cut(s) 786, 1907, 2751, 2912
BssMI GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
BssNAI GTATAC 2 cut(s) 323, 1096
BssT1I CCWWGG 3 cut(s) 1907, 2751, 2912
Bst1107I GTATAC 2 cut(s) 323, 1096
Bst2UI CCWGG 1 cut(s) 787
Bst4CI ACNGT 7 cut(s) 754, 1784, 1825, 1919, 2154, 2259, 3011
Bst6I CTCTTC 7 cut(s) 125, 183, 417, 616, 1569, 1962, 2466
BstAPI GCANNNNNTGC 1 cut(s) 236
BstBAI YACGTR 1 cut(s) 2785
BstBI TTCGAA 2 cut(s) 370, 2420
BstC8I GCNNGC 8 cut(s) 30, 34, 408, 1301, 1458, 1950, 2222, 2226
BstDEI CTNAG 9 cut(s) 564, 863, 1539, 1892, 1986, 2828, 2866, 2957, 3037
BstDSI CCRYGG 1 cut(s) 1907
BstEII GGTNACC 1 cut(s) 638
BstFNI CGCG 3 cut(s) 688, 2004, 2724
BstH2I RGCGCY 1 cut(s) 2619
BstHHI GCGC 3 cut(s) 496, 1259, 2618
BstKTI GATC 7 cut(s) 67, 92, 385, 794, 1591, 2794, 3059
BstMAI GTCTC 5 cut(s) 173, 1213, 1928, 1976, 2869
BstMBI GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
BstMWI GCNNNNNNNGC 7 cut(s) 236, 403, 734, 1373, 1576, 2230, 2986
BstNI CCWGG 1 cut(s) 787
BstNSI RCATGY 1 cut(s) 1396
BstPI GGTNACC 1 cut(s) 638
BstSCI CCNGG 2 cut(s) 785, 2945
BstSLI GKGCMC 1 cut(s) 2269
BstUI CGCG 3 cut(s) 688, 2004, 2724
BstX2I RGATCY 5 cut(s) 64, 791, 1588, 2791, 3056
BstXI CCANNNNNNTGG 1 cut(s) 2411
BstYI RGATCY 5 cut(s) 64, 791, 1588, 2791, 3056
BstZ17I GTATAC 2 cut(s) 323, 1096
Bsu15I ATCGAT 1 cut(s) 381
Bsu36I CCTNAGG 1 cut(s) 1892
BsuI GTATCC 2 cut(s) 823, 3063
BsuRI GGCC 6 cut(s) 397, 1661, 2010, 2676, 2950, 3063
BsuTUI ATCGAT 1 cut(s) 381
BtgI CCRYGG 1 cut(s) 1907
BtgZI GCGATG 3 cut(s) 1169, 2019, 2826
BtsI GCAGTG 2 cut(s) 318, 903
BtsIMutI CAGTG 4 cut(s) 318, 903, 2255, 2879
BtuMI TCGCGA 1 cut(s) 2724
Cac8I GCNNGC 8 cut(s) 30, 34, 408, 1301, 1458, 1950, 2222, 2226
CaiI CAGNNNCTG 2 cut(s) 1306, 1472
CciI TCATGA 1 cut(s) 2463
CfoI GCGC 3 cut(s) 496, 1259, 2618
Cfr10I RCCGGY 2 cut(s) 923, 1426
Cfr13I GGNCC 4 cut(s) 395, 711, 1888, 2675
ClaI ATCGAT 1 cut(s) 381
CseI GACGC 2 cut(s) 677, 2910
Csp6I GTAC 7 cut(s) 465, 975, 1229, 1449, 1780, 1962, 2904
CspAI ACCGGT 1 cut(s) 1426
CspCI CAANNNNNGTGG 2 cut(s) 2340, 2375
CviQI GTAC 7 cut(s) 465, 975, 1229, 1449, 1780, 1962, 2904
DdeI CTNAG 9 cut(s) 564, 863, 1539, 1892, 1986, 2828, 2866, 2957, 3037
DpnI GATC 7 cut(s) 66, 91, 384, 793, 1590, 2793, 3058
DpnII GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
DrdI GACNNNNNNGTC 1 cut(s) 636
DriI GACNNNNNGTC 2 cut(s) 1609, 1894
DseDI GACNNNNNNGTC 1 cut(s) 636
EaeI YGGCCR 3 cut(s) 2008, 2948, 3061
Eam1104I CTCTTC 7 cut(s) 125, 183, 417, 616, 1569, 1962, 2466
Eam1105I GACNNNNNGTC 2 cut(s) 1609, 1894
EarI CTCTTC 7 cut(s) 125, 183, 417, 616, 1569, 1962, 2466
EciI GGCGGA 2 cut(s) 1778, 2243
Ecl136II GAGCTC 2 cut(s) 290, 2220
Eco130I CCWWGG 3 cut(s) 1907, 2751, 2912
Eco24I GRGCYC 2 cut(s) 292, 2222
Eco31I GGTCTC 3 cut(s) 173, 1213, 2869
Eco47I GGWCC 2 cut(s) 711, 1888
Eco53kI GAGCTC 2 cut(s) 290, 2220
Eco57I CTGAAG 4 cut(s) 146, 873, 1276, 1986
Eco72I CACGTG 1 cut(s) 2785
Eco81I CCTNAGG 1 cut(s) 1892
Eco88I CYCGRG 1 cut(s) 591
Eco91I GGTNACC 1 cut(s) 638
EcoICRI GAGCTC 2 cut(s) 290, 2220
EcoO109I RGGNCCY 2 cut(s) 711, 2675
EcoO65I GGTNACC 1 cut(s) 638
EcoRII CCWGG 1 cut(s) 785
EcoT14I CCWWGG 3 cut(s) 1907, 2751, 2912
EcoT38I GRGCYC 2 cut(s) 292, 2222
ErhI CCWWGG 3 cut(s) 1907, 2751, 2912
Esp3I CGTCTC 1 cut(s) 1928
FalI AAGNNNNNCTT 4 cut(s) 1182, 1214, 2194, 2226
FaqI GGGAC 2 cut(s) 697, 2975
FauI CCCGC 2 cut(s) 1449, 1525
FauNDI CATATG 1 cut(s) 1858
FblI GTMKAC 4 cut(s) 47, 322, 603, 1095
FriOI GRGCYC 2 cut(s) 292, 2222
FspAI RTGCGCAY 1 cut(s) 495
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 3 cut(s) 495, 1258, 2617
GsaI CCCAGC 1 cut(s) 1653
HaeII RGCGCY 1 cut(s) 2619
HaeIII GGCC 6 cut(s) 397, 1661, 2010, 2676, 2950, 3063
HapII CCGG 6 cut(s) 924, 1427, 2947, 3030, 3060, 3077
HgaI GACGC 2 cut(s) 677, 2910
HhaI GCGC 3 cut(s) 496, 1259, 2618
Hin6I GCGC 3 cut(s) 494, 1257, 2616
HinP1I GCGC 3 cut(s) 494, 1257, 2616
HincII GTYRAC 2 cut(s) 48, 2766
HindII GTYRAC 2 cut(s) 48, 2766
HindIII AAGCTT 2 cut(s) 1669, 2192
HpaII CCGG 6 cut(s) 924, 1427, 2947, 3030, 3060, 3077
Hpy99I CGWCG 2 cut(s) 720, 1923
HpyCH4III ACNGT 7 cut(s) 754, 1784, 1825, 1919, 2154, 2259, 3011
HpyCH4IV ACGT 2 cut(s) 1129, 2784
HpyF10VI GCNNNNNNNGC 7 cut(s) 236, 403, 734, 1373, 1576, 2230, 2986
HpyF3I CTNAG 9 cut(s) 564, 863, 1539, 1892, 1986, 2828, 2866, 2957, 3037
HpySE526I ACGT 2 cut(s) 1129, 2784
HspAI GCGC 3 cut(s) 494, 1257, 2616
KflI GGGWCCC 1 cut(s) 711
Kpn2I TCCGGA 1 cut(s) 3076
Kzo9I GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
LguI GCTCTTC 2 cut(s) 417, 1569
LmnI GCTCC 3 cut(s) 1508, 1714, 2230
MaeII ACGT 2 cut(s) 1129, 2784
MalI GATC 7 cut(s) 66, 91, 384, 793, 1590, 2793, 3058
MboI GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
MfeI CAATTG 2 cut(s) 729, 1068
MflI RGATCY 5 cut(s) 64, 791, 1588, 2791, 3056
MhlI GDGCHC 3 cut(s) 292, 2222, 2269
MlsI TGGCCA 1 cut(s) 2010
MluNI TGGCCA 1 cut(s) 2010
MlyI GAGTC 4 cut(s) 1619, 2075, 2510, 2767
MmeI TCCRAC 2 cut(s) 819, 1005
Mox20I TGGCCA 1 cut(s) 2010
MroI TCCGGA 1 cut(s) 3076
MroXI GAANNNNTTC 2 cut(s) 1197, 2061
MscI TGGCCA 1 cut(s) 2010
MseI TTAA 3 cut(s) 542, 1038, 1673
MslI CAYNNNNRTG 5 cut(s) 77, 824, 2409, 2969, 2997
Msp20I TGGCCA 1 cut(s) 2010
MspA1I CMGCKG 3 cut(s) 233, 663, 1306
MspI CCGG 6 cut(s) 924, 1427, 2947, 3030, 3060, 3077
MspR9I CCNGG 2 cut(s) 787, 2947
MunI CAATTG 2 cut(s) 729, 1068
MvaI CCWGG 1 cut(s) 787
MvnI CGCG 3 cut(s) 688, 2004, 2724
MwoI GCNNNNNNNGC 7 cut(s) 236, 403, 734, 1373, 1576, 2230, 2986
NciI CCSGG 1 cut(s) 2947
NcoI CCATGG 1 cut(s) 1907
NdeI CATATG 1 cut(s) 1858
NdeII GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
NlaIV GGNNCC 9 cut(s) 55, 712, 713, 793, 1504, 1716, 2253, 2268, 3058
NmuCI GTSAC 4 cut(s) 583, 638, 694, 3040
NruI TCGCGA 1 cut(s) 2724
NsbI TGCGCA 1 cut(s) 495
NspI RCATGY 1 cut(s) 1396
NspV TTCGAA 2 cut(s) 370, 2420
OliI CACNNNNGTG 2 cut(s) 824, 2997
PaeR7I CTCGAG 1 cut(s) 591
PagI TCATGA 1 cut(s) 2463
PciI ACATGT 1 cut(s) 1392
PciSI GCTCTTC 2 cut(s) 417, 1569
PdmI GAANNNNTTC 2 cut(s) 1197, 2061
PfeI GAWTC 9 cut(s) 107, 263, 359, 1016, 1751, 2057, 2728, 2821, 3033
PflFI GACNNNGTC 2 cut(s) 636, 2920
PflMI CCANNNNNTGG 1 cut(s) 577
PinAI ACCGGT 1 cut(s) 1426
PleI GAGTC 4 cut(s) 1618, 2075, 2509, 2766
PmaCI CACGTG 1 cut(s) 2785
PmlI CACGTG 1 cut(s) 2785
PpsI GAGTC 4 cut(s) 1618, 2075, 2509, 2766
Ppu21I YACGTR 1 cut(s) 2785
PpuMI RGGWCCY 1 cut(s) 711
PscI ACATGT 1 cut(s) 1392
Psp124BI GAGCTC 2 cut(s) 292, 2222
Psp5II RGGWCCY 1 cut(s) 711
Psp6I CCWGG 1 cut(s) 785
PspCI CACGTG 1 cut(s) 2785
PspEI GGTNACC 1 cut(s) 638
PspFI CCCAGC 1 cut(s) 1649
PspGI CCWGG 1 cut(s) 785
PspN4I GGNNCC 9 cut(s) 55, 712, 713, 793, 1504, 1716, 2253, 2268, 3058
PspPI GGNCC 4 cut(s) 395, 711, 1888, 2675
PspPPI RGGWCCY 1 cut(s) 711
PstI CTGCAG 6 cut(s) 676, 1222, 1348, 1369, 1702, 2127
PstNI CAGNNNCTG 2 cut(s) 1306, 1472
PsuI RGATCY 5 cut(s) 64, 791, 1588, 2791, 3056
PsyI GACNNNGTC 2 cut(s) 636, 2920
PvuII CAGCTG 2 cut(s) 233, 1306
RruI TCGCGA 1 cut(s) 2724
RsaI GTAC 7 cut(s) 466, 976, 1230, 1450, 1781, 1963, 2905
RsaNI GTAC 7 cut(s) 465, 975, 1229, 1449, 1780, 1962, 2904
RseI CAYNNNNRTG 5 cut(s) 77, 824, 2409, 2969, 2997
SacI GAGCTC 2 cut(s) 292, 2222
SalI GTCGAC 1 cut(s) 46
SapI GCTCTTC 2 cut(s) 417, 1569
SaqAI TTAA 3 cut(s) 542, 1038, 1673
Sau3AI GATC 7 cut(s) 64, 89, 382, 791, 1588, 2791, 3056
Sau96I GGNCC 4 cut(s) 395, 711, 1888, 2675
ScaI AGTACT 1 cut(s) 1781
SchI GAGTC 4 cut(s) 1619, 2075, 2510, 2767
ScrFI CCNGG 2 cut(s) 787, 2947
SduI GDGCHC 3 cut(s) 292, 2222, 2269
Sfr274I CTCGAG 1 cut(s) 591
SfuI TTCGAA 2 cut(s) 370, 2420
SinI GGWCC 2 cut(s) 711, 1888
SlaI CTCGAG 1 cut(s) 591
SmiMI CAYNNNNRTG 5 cut(s) 77, 824, 2409, 2969, 2997
SmlI CTYRAG 3 cut(s) 15, 591, 2608
SmoI CTYRAG 3 cut(s) 15, 591, 2608
SsiI CCGC 9 cut(s) 663, 686, 734, 1456, 1518, 1763, 1774, 2228, 3027
SspI AATATT 2 cut(s) 160, 2089
SstI GAGCTC 2 cut(s) 292, 2222
StyD4I CCNGG 2 cut(s) 785, 2945
StyI CCWWGG 3 cut(s) 1907, 2751, 2912
TaaI ACNGT 7 cut(s) 754, 1784, 1825, 1919, 2154, 2259, 3011
TaiI ACGT 2 cut(s) 1132, 2787
TaqI TCGA 9 cut(s) 47, 370, 381, 592, 1111, 2104, 2420, 2427, 2824
TaqII GACCGA 1 cut(s) 2751
TatI WGTACW 2 cut(s) 1779, 1961
TauI GCSGC 1 cut(s) 737
TfiI GAWTC 9 cut(s) 107, 263, 359, 1016, 1751, 2057, 2728, 2821, 3033
Tru1I TTAA 3 cut(s) 542, 1038, 1673
Tru9I TTAA 3 cut(s) 542, 1038, 1673
TscAI CASTG 4 cut(s) 318, 910, 2262, 2886
TseFI GTSAC 4 cut(s) 583, 638, 694, 3040
Tsp45I GTSAC 4 cut(s) 583, 638, 694, 3040
TspDTI ATGAA 7 cut(s) 170, 1172, 1190, 1302, 1599, 2403, 2421
TspGWI ACGGA 3 cut(s) 779, 1301, 2014
TspRI CASTG 4 cut(s) 318, 910, 2262, 2886
Tth111I GACNNNGTC 2 cut(s) 636, 2920
Van91I CCANNNNNTGG 1 cut(s) 577
VpaK11BI GGWCC 2 cut(s) 711, 1888
XapI RAATTY 4 cut(s) 38, 148, 365, 2696
XceI RCATGY 1 cut(s) 1396
XhoI CTCGAG 1 cut(s) 591
XmiI GTMKAC 4 cut(s) 47, 322, 603, 1095
XmnI GAANNNNTTC 2 cut(s) 1197, 2061
ZrmI AGTACT 1 cut(s) 1781
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.