pycom14g01110

Random slug protein 5-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010748
Physical Location & Seq
Reverse (-)
793225 .. 795531
2307 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 699 bp
ATGTCGTTCAAAAAAGGGGAAAGCACAACAGATAAAGCATTGTCACATGAAGAGCAGCTGGCCAAGATTTATGAAGTGAGGAAGATGATGGGTCCTATTGTGGATAGGCTACCAGCCATTTGTTCAGAAGCTTCAATCTCAAGGTACCTAAGAGCGCGGAACTGGAATACGAAGAAGGCGGGCAAGATGCTGAAAGAAACCATAAAATGGAGGTTGGAGTTCAAGCCCGAGAAAATCCGATGGGAAAACATTACTCAAGAAGCGAAGACAGGAAAAATTTACAGAGCCAATTACCATGACAATTGCGGAAGGACAGTTCTCATCCTGAGGCCTGGTTTTCAGGTAGTTAAATCACACGAAAAGCGTCTACATCAGGAACAAATGGTGTGGCTAATCGACTTTCAACGGTGGAACACGTCAAGCATATCGTTGAAGGCGACTAGGGAAACAGCTAAGATCCTGCAAAATCATTACCCTGAGAGATTGGGAATTGGCATCCTCTATAATCCACCAAAAATATTTGAGTCCTTTTGGACGTTGGTAAAACCATTTCTTGAGCCGAAAACACACAAAAAGATGAAGTTTGTGTACTCGGACAACCCGCAGAGCCAGAAGATAATGGAAGATCGATCAAATTTTCAACATGGACAAGTTGGAATCCACATTTGGCGGGAGAAGTTCAGTTGGTTTCGACTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO CRAL_TRIO_N

Protein Analysis

233

Amino Acids

27.67

Weight (kDa)

9.98

Isoelectric Point (pI)

42.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO PF00650 121 - 199 2.4e-19 CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 144
AccB1I GGYRCC 1 cut(s) 144
AccB7I CCANNNNNTGG 1 cut(s) 207
AccI GTMKAC 1 cut(s) 367
AccII CGCG 1 cut(s) 157
AciI CCGC 5 cut(s) 157, 179, 306, 602, 670
AclWI GGATC 1 cut(s) 451
AcoI YGGCCR 1 cut(s) 60
AcsI RAATTY 2 cut(s) 276, 634
AfaI GTAC 2 cut(s) 146, 590
AfiI CCNNNNNNNGG 1 cut(s) 207
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 6 cut(s) 10, 135, 223, 404, 433, 641
AjiI CACGTC 1 cut(s) 417
AjnI CCWGG 1 cut(s) 331
AjuI GAANNNNNNNTTGG 2 cut(s) 649, 681
AloI GAACNNNNNNTCC 2 cut(s) 300, 332
AluBI AGCT 3 cut(s) 58, 131, 452
AluI AGCT 3 cut(s) 58, 131, 452
AlwI GGATC 1 cut(s) 451
Ama87I CYCGRG 1 cut(s) 227
AoxI GGCC 2 cut(s) 60, 329
ApeKI GCWGC 1 cut(s) 55
ApoI RAATTY 2 cut(s) 276, 634
Asp718I GGTACC 1 cut(s) 144
AspLEI GCGC 1 cut(s) 157
AspS9I GGNCC 1 cut(s) 92
AvaI CYCGRG 1 cut(s) 227
AvaII GGWCC 1 cut(s) 92
AxyI CCTNAGG 1 cut(s) 326
BalI TGGCCA 1 cut(s) 62
BanI GGYRCC 1 cut(s) 144
BarI GAAGNNNNNNTAC 2 cut(s) 572, 604
BbsI GAAGAC 1 cut(s) 272
BbvI GCAGC 1 cut(s) 67
BccI CCATC 2 cut(s) 82, 234
BciT130I CCWGG 1 cut(s) 333
BfaI CTAG 1 cut(s) 441
BisI GCNGC 1 cut(s) 56
BlsI GCNGC 1 cut(s) 57
Bme1390I CCNGG 1 cut(s) 333
Bme18I GGWCC 1 cut(s) 92
BmeT110I CYCGRG 1 cut(s) 227
BmgBI CACGTC 1 cut(s) 417
BmgT120I GGNCC 1 cut(s) 92
BmiI GGNNCC 2 cut(s) 93, 146
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 2 cut(s) 177, 504
BpiI GAAGAC 1 cut(s) 272
BpuEI CTTGAG 3 cut(s) 124, 240, 575
Bsa29I ATCGAT 1 cut(s) 628
Bsc4I CCNNNNNNNGG 1 cut(s) 207
Bse1I ACTGG 1 cut(s) 167
Bse21I CCTNAGG 1 cut(s) 326
BseBI CCWGG 1 cut(s) 333
BseCI ATCGAT 1 cut(s) 628
BseGI GGATG 2 cut(s) 321, 495
BseLI CCNNNNNNNGG 1 cut(s) 207
BseMII CTCAG 2 cut(s) 317, 468
BseNI ACTGG 1 cut(s) 167
BseXI GCAGC 1 cut(s) 67
Bsh1236I CGCG 1 cut(s) 157
BshFI GGCC 2 cut(s) 62, 331
BshNI GGYRCC 1 cut(s) 144
BshVI ATCGAT 1 cut(s) 628
BsiHKCI CYCGRG 1 cut(s) 227
BslI CCNNNNNNNGG 1 cut(s) 207
BsnI GGCC 2 cut(s) 62, 331
BsoBI CYCGRG 1 cut(s) 227
Bsp143I GATC 3 cut(s) 456, 625, 629
BspACI CCGC 5 cut(s) 157, 179, 306, 602, 670
BspANI GGCC 2 cut(s) 62, 331
BspCNI CTCAG 2 cut(s) 318, 469
BspDI ATCGAT 1 cut(s) 628
BspFNI CGCG 1 cut(s) 157
BspLI GGNNCC 2 cut(s) 93, 146
BspPI GGATC 1 cut(s) 451
BspQI GCTCTTC 1 cut(s) 45
BspT107I GGYRCC 1 cut(s) 144
BsrI ACTGG 1 cut(s) 167
BssMI GATC 3 cut(s) 456, 625, 629
Bst2UI CCWGG 1 cut(s) 333
Bst4CI ACNGT 2 cut(s) 316, 408
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 2 cut(s) 60, 181
BstDEI CTNAG 4 cut(s) 149, 326, 453, 477
BstF5I GGATG 2 cut(s) 321, 495
BstFNI CGCG 1 cut(s) 157
BstHHI GCGC 1 cut(s) 157
BstKTI GATC 3 cut(s) 459, 628, 632
BstMBI GATC 3 cut(s) 456, 625, 629
BstNI CCWGG 1 cut(s) 333
BstSCI CCNGG 1 cut(s) 331
BstUI CGCG 1 cut(s) 157
BstV1I GCAGC 1 cut(s) 67
BstV2I GAAGAC 1 cut(s) 272
BstX2I RGATCY 1 cut(s) 456
BstYI RGATCY 1 cut(s) 456
Bsu15I ATCGAT 1 cut(s) 628
Bsu36I CCTNAGG 1 cut(s) 326
BsuRI GGCC 2 cut(s) 62, 331
BsuTUI ATCGAT 1 cut(s) 628
BtrI CACGTC 1 cut(s) 417
BtsCI GGATG 2 cut(s) 321, 495
Cac8I GCNNGC 2 cut(s) 60, 181
CfoI GCGC 1 cut(s) 157
Cfr13I GGNCC 1 cut(s) 92
ClaI ATCGAT 1 cut(s) 628
CseI GACGC 1 cut(s) 353
Csp6I GTAC 2 cut(s) 145, 589
CspCI CAANNNNNGTGG 2 cut(s) 368, 403
CviAII CATG 3 cut(s) 47, 296, 644
CviQI GTAC 2 cut(s) 145, 589
DdeI CTNAG 4 cut(s) 149, 326, 453, 477
DpnI GATC 3 cut(s) 458, 627, 631
DpnII GATC 3 cut(s) 456, 625, 629
EaeI YGGCCR 1 cut(s) 60
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco147I AGGCCT 1 cut(s) 331
Eco47I GGWCC 1 cut(s) 92
Eco81I CCTNAGG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 227
EcoO109I RGGNCCY 1 cut(s) 92
EcoRII CCWGG 1 cut(s) 331
FaeI CATG 3 cut(s) 50, 299, 647
FaiI YATR 8 cut(s) 48, 72, 203, 297, 425, 504, 645, 697
FatI CATG 3 cut(s) 46, 295, 643
FauI CCCGC 3 cut(s) 172, 609, 663
FblI GTMKAC 1 cut(s) 367
Fnu4HI GCNGC 1 cut(s) 56
FokI GGATG 2 cut(s) 308, 482
Fsp4HI GCNGC 1 cut(s) 56
FspBI CTAG 1 cut(s) 441
GlaI GCGC 1 cut(s) 156
GluI GCNGC 1 cut(s) 56
HaeIII GGCC 2 cut(s) 62, 331
HgaI GACGC 1 cut(s) 353
HhaI GCGC 1 cut(s) 157
Hin1II CATG 3 cut(s) 50, 299, 647
Hin6I GCGC 1 cut(s) 155
HinP1I GCGC 1 cut(s) 155
HindIII AAGCTT 1 cut(s) 129
HinfI GANTC 2 cut(s) 524, 657
Hpy166II GTNNAC 2 cut(s) 368, 589
Hpy188I TCNGA 3 cut(s) 127, 239, 595
Hpy188III TCNNGA 4 cut(s) 257, 325, 374, 554
Hpy8I GTNNAC 2 cut(s) 368, 589
HpyAV CCTTC 3 cut(s) 169, 303, 427
HpyCH4III ACNGT 2 cut(s) 316, 408
HpyCH4IV ACGT 2 cut(s) 416, 536
HpyCH4V TGCA 1 cut(s) 463
HpyF3I CTNAG 4 cut(s) 149, 326, 453, 477
HpySE526I ACGT 2 cut(s) 416, 536
Hsp92II CATG 3 cut(s) 50, 299, 647
HspAI GCGC 1 cut(s) 155
KpnI GGTACC 1 cut(s) 148
Kzo9I GATC 3 cut(s) 456, 625, 629
LguI GCTCTTC 1 cut(s) 45
Lsp1109I GCAGC 1 cut(s) 67
LweI GCATC 2 cut(s) 177, 504
MaeI CTAG 1 cut(s) 441
MaeII ACGT 2 cut(s) 416, 536
MaeIII GTNAC 1 cut(s) 42
MalI GATC 3 cut(s) 458, 627, 631
MboI GATC 3 cut(s) 456, 625, 629
MboII GAAGA 6 cut(s) 62, 94, 184, 277, 625, 635
MfeI CAATTG 1 cut(s) 301
MflI RGATCY 1 cut(s) 456
MlsI TGGCCA 1 cut(s) 62
MluCI AATT 5 cut(s) 276, 289, 301, 489, 634
MluNI TGGCCA 1 cut(s) 62
MlyI GAGTC 1 cut(s) 533
MmeI TCCRAC 2 cut(s) 195, 634
MnlI CCTC 4 cut(s) 72, 204, 321, 509
Mox20I TGGCCA 1 cut(s) 62
MscI TGGCCA 1 cut(s) 62
MseI TTAA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 62
MspA1I CMGCKG 1 cut(s) 58
MspR9I CCNGG 1 cut(s) 333
MunI CAATTG 1 cut(s) 301
MvaI CCWGG 1 cut(s) 333
MvnI CGCG 1 cut(s) 157
NdeII GATC 3 cut(s) 456, 625, 629
NlaIII CATG 3 cut(s) 50, 299, 647
NlaIV GGNNCC 2 cut(s) 93, 146
NmuCI GTSAC 1 cut(s) 42
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 45
PcsI WCGNNNNNNNCGW 1 cut(s) 434
PfeI GAWTC 1 cut(s) 657
PflMI CCANNNNNTGG 1 cut(s) 207
PkrI GCNGC 1 cut(s) 57
PleI GAGTC 1 cut(s) 532
PpsI GAGTC 1 cut(s) 532
PpuMI RGGWCCY 1 cut(s) 92
Psp5II RGGWCCY 1 cut(s) 92
Psp6I CCWGG 1 cut(s) 331
PspGI CCWGG 1 cut(s) 331
PspN4I GGNNCC 2 cut(s) 93, 146
PspPI GGNCC 1 cut(s) 92
PspPPI RGGWCCY 1 cut(s) 92
PsuI RGATCY 1 cut(s) 456
PvuII CAGCTG 1 cut(s) 58
RsaI GTAC 2 cut(s) 146, 590
RsaNI GTAC 2 cut(s) 145, 589
SapI GCTCTTC 1 cut(s) 45
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 1 cut(s) 56
Sau3AI GATC 3 cut(s) 456, 625, 629
Sau96I GGNCC 1 cut(s) 92
SchI GAGTC 1 cut(s) 533
ScrFI CCNGG 1 cut(s) 333
SetI ASST 9 cut(s) 60, 133, 146, 150, 215, 345, 419, 454, 539
SfaNI GCATC 2 cut(s) 177, 504
SinI GGWCC 1 cut(s) 92
SmlI CTYRAG 3 cut(s) 139, 255, 554
SmoI CTYRAG 3 cut(s) 139, 255, 554
Sse9I AATT 5 cut(s) 276, 289, 301, 489, 634
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 5 cut(s) 157, 179, 306, 602, 670
SspI AATATT 1 cut(s) 519
SspMI CTAG 1 cut(s) 441
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 1 cut(s) 331
TaaI ACNGT 2 cut(s) 316, 408
TaiI ACGT 2 cut(s) 419, 539
TaqI TCGA 3 cut(s) 396, 628, 691
TasI AATT 5 cut(s) 276, 289, 301, 489, 634
TatI WGTACW 1 cut(s) 588
TfiI GAWTC 1 cut(s) 657
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TseFI GTSAC 1 cut(s) 42
TseI GCWGC 1 cut(s) 55
Tsp45I GTSAC 1 cut(s) 42
TspDTI ATGAA 3 cut(s) 63, 87, 593
Van91I CCANNNNNTGG 1 cut(s) 207
VpaK11BI GGWCC 1 cut(s) 92
XapI RAATTY 2 cut(s) 276, 634
XmiI GTMKAC 1 cut(s) 367
XspI CTAG 1 cut(s) 441
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.