pycom14g02950

Momilactone A

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016365
Physical Location & Seq
Forward (+)
2274454 .. 2275296
843 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g02950.1

Sequence Viewer

Length: 843 bp
ATGTATGTTGAAATTTTCAAAAGTAATTATTATTTTGTAACTGAATGCAGGCTGGAGGGAAAGGTGGCTCTGATAACCGGAGGAGCCAGTGGCATTGGAGAATGCACAGCCAAAATCTTTGTCCAGCATGGGGCCAAAGTCGTCATTGCAGATGTCCAAGATGATCTGGGCCATTCGGTTCGCGAGTCCATCGGGCCATCGGACTGCACGTTCGTCCACTGTGATGTCACAGATGAAGCCCAAATAAAAGATGCCGTACACAAAGCCGTTGCCGCATACGGAAAACTAGACATCATGTTCAACAACGCAGGCGTAGTTGACCCAAACAAAGCCCGAATCATCGACAATGAAAAGGCAGATTTCGAGCGCGTGCTTAGCATAAATGTCACCGGAGTTTTCCTCGGCATAAAGCATGCTGCCCAAGCCATGATTCCTACGCGGACGGGCAGCATAATTTCAACTGCTAGTATAAGTTCATATGTTGGTGGTGCAGCCTCACATGCATATTGTTGTTCCAAGCATGCAGTTAATGGCTTGACAAAAAATGCAGCCGTTGAGTTAGGTCAGTTCGGGATCCGAGTGAACTGCTTGTCGCCGTACGCGCTTGCAACGCCGTTGGCTCGGAATTTTGTGGGGGTTGAGGATGAGGAGCTTGAGAATGTGATGGGATCTCTAGCCAACTTGAAGGGCGTGACACTTAAGGCGGTGGATGTGGCAAATGCAGCTCTATATTTGGCAAGTGATGAGGCTAGGTATATTAGTGGGCATAATCTCTTGATAGATGGGGGTTTCAGCATCCTTAATCCCTCTTTCGAAATGTTTCAGTACCCTCAAGAGCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
adh_short_C2

Protein Analysis

281

Amino Acids

29.9

Weight (kDa)

5.35

Isoelectric Point (pI)

21.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 21 - 209 3.2e-52 short chain dehydrogenase
KR PF08659 23 - 181 3.4e-12 KR domain
adh_short_C2 PF13561 29 - 265 6.7e-65 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016365)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g19720 FvH4_6g19720
malus_domestica MD14G1033500.v1.1
prunus_persica Prupe.7G099500_v2.0.a1
pyrus_communis pycom05g19960 pycom14g02950
rosa_chinensis RchiOBHm_Chr3g0475081
rosa_laevigata RLG00000023865
rosa_multiflora Rmu_sc0003093.1_g000019
rosa_roxburghii Rroxscaffold_6G00406580
rosa_rugosa Rorug03G0146600
rosa_samantha Rh3AG196400 Rh3BG226400 Rh3DG221800
rosa_wichuraiana Rw3G017870

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 183, 369, 439, 602
AciI CCGC 3 cut(s) 273, 439, 704
AclWI GGATC 3 cut(s) 568, 581, 676
AcsI RAATTY 2 cut(s) 12, 625
AfaI GTAC 3 cut(s) 258, 599, 827
AfiI CCNNNNNNNGG 1 cut(s) 130
AflII CTTAAG 1 cut(s) 698
AgsI TTSAA 5 cut(s) 11, 19, 301, 459, 685
AjuI GAANNNNNNNTTGG 2 cut(s) 414, 446
AloI GAACNNNNNNTCC 2 cut(s) 194, 226
AluBI AGCT 2 cut(s) 652, 725
AluI AGCT 2 cut(s) 652, 725
AlwI GGATC 3 cut(s) 568, 581, 676
AoxI GGCC 3 cut(s) 132, 169, 194
ApeKI GCWGC 5 cut(s) 416, 447, 491, 548, 722
ApoI RAATTY 2 cut(s) 12, 625
ArsI GACNNNNNNTTYG 4 cut(s) 105, 137, 194, 226
Asp700I GAANNNNTTC 1 cut(s) 819
AspLEI GCGC 2 cut(s) 369, 604
AspS9I GGNCC 3 cut(s) 132, 169, 194
AsuHPI GGTGA 1 cut(s) 379
AsuII TTCGAA 1 cut(s) 813
BamHI GGATCC 1 cut(s) 573
BbvI GCAGC 5 cut(s) 403, 459, 503, 560, 734
BccI CCATC 4 cut(s) 197, 205, 658, 776
BceAI ACGGC 5 cut(s) 239, 251, 536, 580, 598
BcgI CGANNNNNNTGC 2 cut(s) 567, 601
BfaI CTAG 4 cut(s) 287, 465, 674, 750
BfrI CTTAAG 1 cut(s) 698
BisI GCNGC 6 cut(s) 273, 417, 448, 492, 549, 723
BlpI GCTNAGC 1 cut(s) 374
BlsI GCNGC 6 cut(s) 274, 418, 449, 493, 550, 724
BmgT120I GGNCC 3 cut(s) 132, 169, 194
BmiI GGNNCC 3 cut(s) 85, 133, 575
BmsI GCATC 2 cut(s) 241, 804
BplI GAGNNNNNCTC 2 cut(s) 384, 416
BpmI CTGGAG 1 cut(s) 74
Bpu1102I GCTNAGC 1 cut(s) 374
Bpu14I TTCGAA 1 cut(s) 813
BpuEI CTTGAG 2 cut(s) 674, 816
BsaJI CCNNGG 1 cut(s) 400
BsaWI WCCGGW 2 cut(s) 77, 389
Bsc4I CCNNNNNNNGG 1 cut(s) 130
Bse1I ACTGG 1 cut(s) 87
Bse3DI GCAATG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 400
BseGI GGATG 3 cut(s) 649, 715, 795
BseLI CCNNNNNNNGG 1 cut(s) 130
BseMI GCAATG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 87
BseRI GAGGAG 2 cut(s) 96, 662
BseXI GCAGC 5 cut(s) 403, 459, 503, 560, 734
BsgI GTGCAG 2 cut(s) 190, 510
Bsh1236I CGCG 4 cut(s) 183, 369, 439, 602
BshFI GGCC 3 cut(s) 134, 171, 196
BsiSI CCGG 2 cut(s) 78, 390
BsiWI CGTACG 1 cut(s) 597
BslI CCNNNNNNNGG 1 cut(s) 130
BsmI GAATGC 2 cut(s) 50, 107
BsnI GGCC 3 cut(s) 134, 171, 196
Bsp119I TTCGAA 1 cut(s) 813
Bsp143I GATC 3 cut(s) 163, 573, 668
Bsp1720I GCTNAGC 1 cut(s) 374
Bsp68I TCGCGA 1 cut(s) 183
BspACI CCGC 3 cut(s) 273, 439, 704
BspANI GGCC 3 cut(s) 134, 171, 196
BspFNI CGCG 4 cut(s) 183, 369, 439, 602
BspLI GGNNCC 3 cut(s) 85, 133, 575
BspPI GGATC 3 cut(s) 568, 581, 676
BspT104I TTCGAA 1 cut(s) 813
BspTI CTTAAG 1 cut(s) 698
BsrDI GCAATG 1 cut(s) 144
BsrI ACTGG 1 cut(s) 87
BssECI CCNNGG 1 cut(s) 400
BssMI GATC 3 cut(s) 163, 573, 668
Bst4CI ACNGT 1 cut(s) 221
BstAFI CTTAAG 1 cut(s) 698
BstBI TTCGAA 1 cut(s) 813
BstC8I GCNNGC 6 cut(s) 50, 310, 371, 414, 522, 606
BstDEI CTNAG 1 cut(s) 374
BstF5I GGATG 3 cut(s) 649, 715, 795
BstFNI CGCG 4 cut(s) 183, 369, 439, 602
BstHHI GCGC 2 cut(s) 369, 604
BstKTI GATC 3 cut(s) 166, 576, 671
BstMBI GATC 3 cut(s) 163, 573, 668
BstMWI GCNNNNNNNGC 7 cut(s) 272, 375, 422, 500, 601, 610, 722
BstNSI RCATGY 3 cut(s) 416, 503, 524
BstUI CGCG 4 cut(s) 183, 369, 439, 602
BstV1I GCAGC 5 cut(s) 403, 459, 503, 560, 734
BstX2I RGATCY 2 cut(s) 573, 668
BstYI RGATCY 2 cut(s) 573, 668
BsuRI GGCC 3 cut(s) 134, 171, 196
BtsCI GGATG 3 cut(s) 649, 715, 795
BtsIMutI CAGTG 2 cut(s) 94, 217
BtuMI TCGCGA 1 cut(s) 183
Cac8I GCNNGC 6 cut(s) 50, 310, 371, 414, 522, 606
CfoI GCGC 2 cut(s) 369, 604
Cfr13I GGNCC 3 cut(s) 132, 169, 194
Csp6I GTAC 3 cut(s) 257, 598, 826
CviAII CATG 6 cut(s) 128, 295, 413, 427, 500, 521
CviQI GTAC 3 cut(s) 257, 598, 826
DdeI CTNAG 1 cut(s) 374
DpnI GATC 3 cut(s) 165, 575, 670
DpnII GATC 3 cut(s) 163, 573, 668
EcoT22I ATGCAT 1 cut(s) 505
FaeI CATG 6 cut(s) 131, 298, 416, 430, 503, 524
FatI CATG 6 cut(s) 127, 294, 412, 426, 499, 520
FauNDI CATATG 1 cut(s) 478
Fnu4HI GCNGC 6 cut(s) 273, 417, 448, 492, 549, 723
FokI GGATG 3 cut(s) 656, 722, 782
Fsp4HI GCNGC 6 cut(s) 273, 417, 448, 492, 549, 723
FspBI CTAG 4 cut(s) 287, 465, 674, 750
GlaI GCGC 2 cut(s) 368, 603
GluI GCNGC 6 cut(s) 273, 417, 448, 492, 549, 723
GsuI CTGGAG 1 cut(s) 74
HaeIII GGCC 3 cut(s) 134, 171, 196
HapII CCGG 2 cut(s) 78, 390
HhaI GCGC 2 cut(s) 369, 604
Hin1II CATG 6 cut(s) 131, 298, 416, 430, 503, 524
Hin6I GCGC 2 cut(s) 367, 602
HinP1I GCGC 2 cut(s) 367, 602
HincII GTYRAC 1 cut(s) 319
HindII GTYRAC 1 cut(s) 319
HinfI GANTC 3 cut(s) 185, 336, 430
HpaII CCGG 2 cut(s) 78, 390
HphI GGTGA 1 cut(s) 379
Hpy166II GTNNAC 4 cut(s) 217, 259, 319, 583
Hpy188I TCNGA 4 cut(s) 72, 202, 578, 624
Hpy188III TCNNGA 4 cut(s) 182, 571, 775, 833
Hpy8I GTNNAC 4 cut(s) 217, 259, 319, 583
HpyAV CCTTC 1 cut(s) 679
HpyCH4III ACNGT 1 cut(s) 221
HpyCH4IV ACGT 1 cut(s) 209
HpyF10VI GCNNNNNNNGC 7 cut(s) 272, 375, 422, 500, 601, 610, 722
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 1 cut(s) 209
Hsp92II CATG 6 cut(s) 131, 298, 416, 430, 503, 524
HspAI GCGC 2 cut(s) 367, 602
Kzo9I GATC 3 cut(s) 163, 573, 668
LmnI GCTCC 2 cut(s) 83, 649
LpnPI CCDG 8 cut(s) 34, 38, 91, 100, 137, 152, 294, 403
Lsp1109I GCAGC 5 cut(s) 403, 459, 503, 560, 734
LweI GCATC 2 cut(s) 241, 804
MaeI CTAG 4 cut(s) 287, 465, 674, 750
MaeII ACGT 1 cut(s) 209
MaeIII GTNAC 4 cut(s) 37, 226, 385, 691
MalI GATC 3 cut(s) 165, 575, 670
MboI GATC 3 cut(s) 163, 573, 668
MflI RGATCY 2 cut(s) 573, 668
MluCI AATT 4 cut(s) 12, 25, 453, 625
MlyI GAGTC 1 cut(s) 194
MnlI CCTC 9 cut(s) 49, 74, 410, 505, 634, 640, 739, 817, 840
Mph1103I ATGCAT 1 cut(s) 505
MroXI GAANNNNTTC 1 cut(s) 819
MseI TTAA 3 cut(s) 528, 699, 801
MslI CAYNNNNRTG 1 cut(s) 222
MspCI CTTAAG 1 cut(s) 698
MspI CCGG 2 cut(s) 78, 390
Mva1269I GAATGC 2 cut(s) 50, 107
MvnI CGCG 4 cut(s) 183, 369, 439, 602
MwoI GCNNNNNNNGC 7 cut(s) 272, 375, 422, 500, 601, 610, 722
NdeI CATATG 1 cut(s) 478
NdeII GATC 3 cut(s) 163, 573, 668
NlaIII CATG 6 cut(s) 131, 298, 416, 430, 503, 524
NlaIV GGNNCC 3 cut(s) 85, 133, 575
NmeAIII GCCGAG 1 cut(s) 381
NmuCI GTSAC 3 cut(s) 226, 385, 691
NruI TCGCGA 1 cut(s) 183
NsiI ATGCAT 1 cut(s) 505
NspI RCATGY 3 cut(s) 416, 503, 524
NspV TTCGAA 1 cut(s) 813
PaeI GCATGC 2 cut(s) 416, 524
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PctI GAATGC 2 cut(s) 50, 107
PdmI GAANNNNTTC 1 cut(s) 819
PfeI GAWTC 2 cut(s) 336, 430
Pfl23II CGTACG 1 cut(s) 597
PkrI GCNGC 6 cut(s) 274, 418, 449, 493, 550, 724
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PspLI CGTACG 1 cut(s) 597
PspN4I GGNNCC 3 cut(s) 85, 133, 575
PspPI GGNCC 3 cut(s) 132, 169, 194
PsuI RGATCY 2 cut(s) 573, 668
RruI TCGCGA 1 cut(s) 183
RsaI GTAC 3 cut(s) 258, 599, 827
RsaNI GTAC 3 cut(s) 257, 598, 826
RseI CAYNNNNRTG 1 cut(s) 222
SaqAI TTAA 3 cut(s) 528, 699, 801
SatI GCNGC 6 cut(s) 273, 417, 448, 492, 549, 723
Sau3AI GATC 3 cut(s) 163, 573, 668
Sau96I GGNCC 3 cut(s) 132, 169, 194
SchI GAGTC 1 cut(s) 194
SetI ASST 6 cut(s) 66, 212, 565, 654, 727, 755
SfaNI GCATC 2 cut(s) 241, 804
SfuI TTCGAA 1 cut(s) 813
SmiMI CAYNNNNRTG 1 cut(s) 222
SmlI CTYRAG 3 cut(s) 653, 698, 831
SmoI CTYRAG 3 cut(s) 653, 698, 831
SphI GCATGC 2 cut(s) 416, 524
Sse9I AATT 4 cut(s) 12, 25, 453, 625
SsiI CCGC 3 cut(s) 273, 439, 704
SspMI CTAG 4 cut(s) 287, 465, 674, 750
TaaI ACNGT 1 cut(s) 221
TaiI ACGT 1 cut(s) 212
TaqI TCGA 3 cut(s) 342, 363, 813
TasI AATT 4 cut(s) 12, 25, 453, 625
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 2 cut(s) 336, 430
Tru1I TTAA 3 cut(s) 528, 699, 801
Tru9I TTAA 3 cut(s) 528, 699, 801
TscAI CASTG 2 cut(s) 94, 224
TseFI GTSAC 3 cut(s) 226, 385, 691
TseI GCWGC 5 cut(s) 416, 447, 491, 548, 722
Tsp45I GTSAC 3 cut(s) 226, 385, 691
TspDTI ATGAA 3 cut(s) 249, 363, 465
TspGWI ACGGA 1 cut(s) 294
TspRI CASTG 2 cut(s) 94, 224
Vha464I CTTAAG 1 cut(s) 698
XapI RAATTY 2 cut(s) 12, 625
XceI RCATGY 3 cut(s) 416, 503, 524
XmnI GAANNNNTTC 1 cut(s) 819
XspI CTAG 4 cut(s) 287, 465, 674, 750
Zsp2I ATGCAT 1 cut(s) 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.