Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0005404

Physical Location & Seq

Genomic Coordinates Reverse (-)

6437239 .. 6446371

Gene Structure 9133 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2592 bp

ATGGCGACGGAGCAAAACATGAGTAGCTGGACCGATCTGCTCCACTCCTCCACCAAGCTTCTCGAACAAGCGGCGCCTTCCGCTCAGTTCCCTCCGCTACAGAGGAATTTAGATCAGTTAGAAACATTATCAAAGAAACTCAAGGCGAAAACTCTACGAAATGAGGCTCCTCAACAATCCGTTGCTGCCACAAGGCTTCTTGCACGTGAGGGACTAAATGCAGAGCAGCTTGCACGTGACCTTAAGTCCTTTGAATTGAAGACAACATTTGAAGATGTTTTTCCGTCTGAAGCAACAAATGTTGAAGAGTATCTGCAGCAGGTCCAACAAATGGCAATGGTCTCGGCTCTTCAAGAAGCTCAGAAGGATAACCTTAGAAGTTTTAATGATTACATGTTAAAAGTTTTGGAGGAGGATTGGCAAAAGGAAAAACGTGATCTCCTTCAAAGCTTAAGCCAGATTTCGACATTACCCAGGACCAACATGACTTATACTAGTAGCGGTGGTAGTCATTCTGGTAAAATAGCATCCATAACTTCTAGCCCTCAAGTTTCATCTTCTCCATCTAGCATGGAGCTTGTACATTTAGAAAGCAAGCCCATCCGTGACAAGAAAGCCTCTATCTATGCTGAAGTTGTAAAAAGTCTGAATAATGCAAGGCAGCATGGATTACTGTTTAAGCCCGCCACAGCTTTCAATGGTGCTTATGAGAGTTTAGGCCTTGATGTATCTGGTGGAAAATCAGTTAACATGCAGAAGATATGGCACCTTCTTCAGACGTTGATGGGTGAAGATATAACCCTACTGCGTAGTGTTTCAAAAAGGATGTCATTAGTTATTGGGGCAAGGCGCCACCTAGAATGGGGGCATGAAAAATACATCATGGATACAATTCAAAGTCATCCGGTGCAGGCTGCCCTTGGAGGAGTTGTTGGAAATTTACAAAGAATTCATGCCTTTCTTCGGATTCGTTTAAGAGATTATGGAGTTCTAGATTTTGATGCAGGCGATGCTCGTCGGCAGCCTCCTGTTGATACCACTTGGCAGCAGATCTACTTTTGCTTGAGAACTGGGTATTACAATGAAGCAAGATCTGTTGCTCTGTCATCTCGTGCTTCACACCAGTTTGCTCCTCTGCTTACGGAGTGGATTAATACTGGGGGTATGGTGCCTGCGGCCATTGCAGCTTCTGCTTCAGAAGAATGTGAAAAAATGTTAAGAATGGTTGATCGTGTGGGCCGAGCTGCATATGACAAGAAAAAGTTGTTATTATATGCTCTAGTATCTGGTTCTCGTAGGCAAATCGACCACCTACTAAGAGATCTACCAACCCTTTTCAACACTATAGAGGATATCTTGTGGTTCAAATTATCAGCTGTAAGAGATTGCCCTGGTGGAGCTGCACCCATTGTTATGAATGAGAGCTTGGTACCATACACATTGGATGATTTGCAGATTTACTTAAATAAATTTGACCCATCATATTATACAAAAAATGGAAAGGACCCCCTTGTGTACCCATATGTTTTGCTTTTAAGCATCCAGTTGATACCAGGTGTGCTATACTTGTTTAAAGAAACTGGAGATGAGGGATATAACATTGATGCTGCCCACATATCAATTGTGCTAGCAGACCATGGGGTCCTTTCTGAAGGTGCTGGTGCTGGACAGAAAATGGGTGTGATGGACGCTTATGCAGAGGCATCAAGCATAATCAGGCAGTATGGATCTGTGTATTTACGTCTTGGTAATCTATCAATGGCATTAGAATATTATGCACAAGCTGCTGCTGCGGTTGGTGGTGGGGAGTTGTCATGGTCTGGAAGAGGTAACGTTGATCAGCAAAGGCAGAGAAATTTGATGCTGAAGCAGCTCCTTACAGAGCTGTTGTTGCGGGATGGTGGGATATATTTATTACTTGGTTCAAGAGGTGCCGGAGAAGAAGGTGAATTGGGTCGATTTTTGACTGATGCAAAAGCAAGGCAACAATTTCTTCTTGAAGCTGCACACCAATGTCAAGAAGCTGGGTTGTATGAAAAGTCTATAGAAATTCAGAAACGAATAGGAGCATTCTCGATGGCTTTGGATACTATTAACAAGTGTCTCTCTGAAGCAATTTGTGCCCTTTCACGTGGTAGATTGGATGGTGATAGTCGAACTGCTGGCCTCATTCAATCTGGAAATGAGATCTTGGAAATGCACAAATATTTTCCTGATATAAGTCCTCAAGAGAGGGAAAGTGTTTCTGAGCAGTACATTGTTTTAAGACAACTTGAGGTCGTATTGTCAATCCATAAATTGGCAATAGGAGGCCGTTATGCTGATGCTTTAAGGGAGGTTGCCAGGCTTCAATTTCTTCCATTAGTTCCTGGGACACCAGATGCGACCACCGATGTCTTCCAGAATTTATCTCCGCATGTCCAAGCCTGTGTTCCAGACCTCTTGAAGGTTGCCCTCACTTGTCTGGACAACATGGTGGATTCTGATGGATCGCTTCGTGCCTTGAGGGCTAAGATTGCTAGCTTCATTGCAAATAATTCCAATAGGAACTGGCCTCGTGATTTGTACGAGAGAGTTGCCCGGAGCTTGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005198 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005635 GO:0005643 GO:0006403 GO:0006405 GO:0006406 GO:0006606 GO:0006611 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0006997 GO:0006999 GO:0008104 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0012505 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016973 GO:0017038 GO:0017056 GO:0022607 GO:0031503 GO:0031967 GO:0031975 GO:0032991 GO:0033036 GO:0033365 GO:0034504 GO:0034613 GO:0034622 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043933 GO:0044085 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0045184 GO:0046907 GO:0046931 GO:0050657 GO:0050658 GO:0051028 GO:0051168 GO:0051169 GO:0051170 GO:0051179 GO:0051234 GO:0051236 GO:0051292 GO:0051641 GO:0051649 GO:0065003 GO:0070727 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0072594

KEGG Pathways

Metabolic & Signaling

ko03013 map03013

Pfam Domains

Protein Families

Nic96

Protein Analysis

864

Amino Acids

95.9

Weight (kDa)

6.53

Isoelectric Point (pI)

47.99

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Nic96	PF04097	250 - 857	6e-96	Nup93/Nic96

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0005404)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G41620 AT3G57350
fragaria_vesca	FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390 FvH4_6g24390
malus_domestica	MD00G1107400.v1.1 MD12G1080600.v1.1 MD14G1076700.v1.1
prunus_persica	Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1 Prupe.7G054800_v2.0.a1
pyrus_communis	pycom12g06710 pycom14g06500
rosa_chinensis	RchiOBHm_Chr3g0483381
rosa_laevigata	RLG00000023288
rosa_multiflora	Rmu_sc0006349.1_g000012
rosa_roxburghii	Rroxscaffold_6G00398400
rosa_rugosa	Rorug03G0205600
rosa_samantha	Rh3AG254000 Rh3CG288100 Rh3DG283900
rosa_wichuraiana	Rw0G023710

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1640
Acc36I	ACCTGC	1 cut(s)	310
Acc65I	GGTACC	1 cut(s)	1429
AccB1I	GGYRCC	6 cut(s)	73, 765, 849, 1168, 1429, 1931
AccB7I	CCANNNNNTGG	2 cut(s)	331, 2299
AccBSI	CCGCTC	1 cut(s)	83
AciI	CCGC	9 cut(s)	71, 81, 95, 501, 684, 1175, 1793, 1894, 2414
AclI	AACGTT	1 cut(s)	1833
AclWI	GGATC	2 cut(s)	1735, 2497
AcoI	YGGCCR	1 cut(s)	1176
AcsI	RAATTY	7 cut(s)	106, 937, 948, 1469, 1855, 2049, 2404
AcuI	CTGAAG	7 cut(s)	309, 651, 758, 1179, 1671, 1886, 2130
AcvI	CACGTG	3 cut(s)	206, 236, 2132
AcyI	GRCGYC	2 cut(s)	74, 850
AfaI	GTAC	5 cut(s)	582, 1431, 1517, 2255, 2567
AfiI	CCNNNNNNNGG	5 cut(s)	331, 862, 963, 2299, 2446
AflII	CTTAAG	2 cut(s)	242, 451
AflIII	ACRYGT	1 cut(s)	393
AhdI	GACNNNNNGTC	1 cut(s)	244
AhlI	ACTAGT	1 cut(s)	494
AjnI	CCWGG	5 cut(s)	473, 1390, 1552, 2342, 2368
AjuI	GAANNNNNNNTTGG	4 cut(s)	1409, 1441, 2415, 2447
Alw26I	GTCTC	2 cut(s)	346, 2108
AlwI	GGATC	2 cut(s)	1735, 2497
AlwNI	CAGNNNCTG	1 cut(s)	1190
AoxI	GGCC	6 cut(s)	718, 1176, 1237, 2164, 2311, 2552
ApoI	RAATTY	7 cut(s)	106, 937, 948, 1469, 1855, 2049, 2404
ArsI	GACNNNNNNTTYG	2 cut(s)	812, 844
AseI	ATTAAT	1 cut(s)	1152
Asp718I	GGTACC	1 cut(s)	1429
AspLEI	GCGC	2 cut(s)	76, 852
AspS9I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
AsuC2I	CCSGG	1 cut(s)	2581
AsuHPI	GGTGA	3 cut(s)	800, 1958, 2159
AsuNHI	GCTAGC	2 cut(s)	1627, 2519
AvaII	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
BaeGI	GKGCMC	1 cut(s)	2125
BanI	GGYRCC	6 cut(s)	73, 765, 849, 1168, 1429, 1931
BauI	CACGAG	2 cut(s)	1110, 2556
BbrPI	CACGTG	3 cut(s)	206, 236, 2132
BbsI	GAAGAC	2 cut(s)	266, 2389
BccI	CCATC	9 cut(s)	571, 608, 778, 1486, 1678, 1892, 2071, 2138, 2480
BceAI	ACGGC	1 cut(s)	2298
BcgI	CGANNNNNNTGC	4 cut(s)	324, 358, 2558, 2592
BciT130I	CCWGG	5 cut(s)	475, 1392, 1554, 2344, 2370
BciVI	GTATCC	2 cut(s)	880, 2080
BclI	TGATCA	1 cut(s)	1837
BcnI	CCSGG	1 cut(s)	2581
BcoDI	GTCTC	2 cut(s)	346, 2108
BcuI	ACTAGT	1 cut(s)	494
BfaI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2520
BfmI	CTRYAG	4 cut(s)	98, 314, 1344, 2043
BfoI	RGCGCY	2 cut(s)	77, 853
BfrI	CTTAAG	2 cut(s)	242, 451
BfuAI	ACCTGC	1 cut(s)	310
BfuI	GTATCC	2 cut(s)	880, 2080
BglI	GCCNNNNNGGC	1 cut(s)	2507
BglII	AGATCT	4 cut(s)	1050, 1091, 1321, 2187
Bme1390I	CCNGG	6 cut(s)	475, 1392, 1554, 2344, 2370, 2581
Bme18I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
BmeRI	GACNNNNNGTC	1 cut(s)	244
BmgT120I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
BmiI	GGNNCC	9 cut(s)	75, 168, 767, 851, 1170, 1431, 1506, 1643, 1933
BmrFI	CCNGG	6 cut(s)	475, 1392, 1554, 2344, 2370, 2581
BmrI	ACTGGG	2 cut(s)	1080, 1167
BmtI	GCTAGC	2 cut(s)	1631, 2523
BmuI	ACTGGG	2 cut(s)	1080, 1167
BpiI	GAAGAC	2 cut(s)	266, 2389
BpmI	CTGGAG	1 cut(s)	1602
BpuEI	CTTGAG	6 cut(s)	125, 531, 1084, 2211, 2294, 2524
BpuMI	CCSGG	1 cut(s)	2581
BsaAI	YACGTR	3 cut(s)	206, 236, 2132
BsaHI	GRCGYC	2 cut(s)	74, 850
BsaI	GGTCTC	1 cut(s)	346
BsaJI	CCNNGG	5 cut(s)	473, 919, 1390, 1636, 2369
BsaWI	WCCGGW	1 cut(s)	904
BsaXI	ACNNNNNCTCC	4 cut(s)	423, 453, 915, 945
Bsc4I	CCNNNNNNNGG	5 cut(s)	331, 862, 963, 2299, 2446
Bse1I	ACTGG	6 cut(s)	1075, 1123, 1162, 1543, 1585, 2555
Bse3DI	GCAATG	3 cut(s)	342, 1179, 2526
BseBI	CCWGG	5 cut(s)	475, 1392, 1554, 2344, 2370
BseDI	CCNNGG	5 cut(s)	473, 919, 1390, 1636, 2369
BseGI	GGATG	8 cut(s)	527, 600, 831, 901, 1450, 1539, 1903, 2149
BseLI	CCNNNNNNNGG	5 cut(s)	331, 862, 963, 2299, 2446
BseMI	GCAATG	3 cut(s)	342, 1179, 2526
BseMII	CTCAG	3 cut(s)	98, 374, 2238
BseNI	ACTGG	6 cut(s)	1075, 1123, 1162, 1543, 1585, 2555
BseRI	GAGGAG	5 cut(s)	37, 159, 425, 939, 1122
BseSI	GKGCMC	1 cut(s)	2125
BseYI	CCCAGC	1 cut(s)	2024
BsgI	GTGCAG	3 cut(s)	929, 1386, 1989
BshFI	GGCC	6 cut(s)	720, 1178, 1239, 2166, 2313, 2554
BshNI	GGYRCC	6 cut(s)	73, 765, 849, 1168, 1429, 1931
BsiSI	CCGG	3 cut(s)	905, 1935, 2581
BslFI	GGGAC	2 cut(s)	225, 2386
BslI	CCNNNNNNNGG	5 cut(s)	331, 862, 963, 2299, 2446
BsmAI	GTCTC	2 cut(s)	346, 2108
BsmFI	GGGAC	2 cut(s)	225, 2386
BsmI	GAATGC	1 cut(s)	2069
BsnI	GGCC	6 cut(s)	720, 1178, 1239, 2166, 2313, 2554
Bso31I	GGTCTC	1 cut(s)	346
Bsp1286I	GDGCHC	1 cut(s)	2125
Bsp1407I	TGTACA	1 cut(s)	580
Bsp19I	CCATGG	1 cut(s)	1636
BspACI	CCGC	9 cut(s)	71, 81, 95, 501, 684, 1175, 1793, 1894, 2414
BspANI	GGCC	6 cut(s)	720, 1178, 1239, 2166, 2313, 2554
BspCNI	CTCAG	3 cut(s)	97, 373, 2239
BspLI	GGNNCC	9 cut(s)	75, 168, 767, 851, 1170, 1431, 1506, 1643, 1933
BspMAI	CTGCAG	1 cut(s)	318
BspMI	ACCTGC	1 cut(s)	310
BspOI	GCTAGC	2 cut(s)	1631, 2523
BspPI	GGATC	2 cut(s)	1735, 2497
BspQI	GCTCTTC	1 cut(s)	354
BspT107I	GGYRCC	6 cut(s)	73, 765, 849, 1168, 1429, 1931
BspTI	CTTAAG	2 cut(s)	242, 451
BspTNI	GGTCTC	1 cut(s)	346
BsrBI	CCGCTC	1 cut(s)	83
BsrDI	GCAATG	3 cut(s)	342, 1179, 2526
BsrGI	TGTACA	1 cut(s)	580
BsrI	ACTGG	6 cut(s)	1075, 1123, 1162, 1543, 1585, 2555
BssECI	CCNNGG	5 cut(s)	473, 919, 1390, 1636, 2369
BssNI	GRCGYC	2 cut(s)	74, 850
BssSI	CACGAG	2 cut(s)	1110, 2556
BssT1I	CCWWGG	2 cut(s)	919, 1636
Bst2BI	CACGAG	2 cut(s)	1110, 2556
Bst2UI	CCWGG	5 cut(s)	475, 1392, 1554, 2344, 2370
Bst4CI	ACNGT	1 cut(s)	675
Bst6I	CTCTTC	3 cut(s)	300, 354, 1819
BstACI	GRCGYC	2 cut(s)	74, 850
BstAFI	CTTAAG	2 cut(s)	242, 451
BstAPI	GCANNNNNTGC	4 cut(s)	1010, 1190, 1784, 2120
BstAUI	TGTACA	1 cut(s)	580
BstBAI	YACGTR	3 cut(s)	206, 236, 2132
BstC8I	GCNNGC	9 cut(s)	231, 596, 684, 912, 1006, 1173, 1629, 2164, 2521
BstDEI	CTNAG	6 cut(s)	84, 360, 374, 1316, 2247, 2511
BstDSI	CCRYGG	1 cut(s)	1636
BstENI	CCTNNNNNAGG	1 cut(s)	2444
BstF5I	GGATG	8 cut(s)	527, 600, 831, 901, 1450, 1539, 1903, 2149
BstH2I	RGCGCY	2 cut(s)	77, 853
BstHHI	GCGC	2 cut(s)	76, 852
BstMAI	GTCTC	2 cut(s)	346, 2108
BstNI	CCWGG	5 cut(s)	475, 1392, 1554, 2344, 2370
BstNSI	RCATGY	3 cut(s)	397, 754, 2420
BstSCI	CCNGG	6 cut(s)	473, 1390, 1552, 2342, 2368, 2579
BstSFI	CTRYAG	4 cut(s)	98, 314, 1344, 2043
BstSLI	GKGCMC	1 cut(s)	2125
BstV2I	GAAGAC	2 cut(s)	266, 2389
BstX2I	RGATCY	5 cut(s)	1050, 1091, 1321, 1727, 2187
BstYI	RGATCY	5 cut(s)	1050, 1091, 1321, 1727, 2187
BsuI	GTATCC	2 cut(s)	880, 2080
BsuRI	GGCC	6 cut(s)	720, 1178, 1239, 2166, 2313, 2554
BtgI	CCRYGG	1 cut(s)	1636
BtgZI	GCGATG	1 cut(s)	1023
BtsCI	GGATG	8 cut(s)	527, 600, 831, 901, 1450, 1539, 1903, 2149
BveI	ACCTGC	1 cut(s)	310
Cac8I	GCNNGC	9 cut(s)	231, 596, 684, 912, 1006, 1173, 1629, 2164, 2521
CaiI	CAGNNNCTG	1 cut(s)	1190
CfoI	GCGC	2 cut(s)	76, 852
Cfr13I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
CseI	GACGC	1 cut(s)	1697
CsiI	ACCWGGT	1 cut(s)	1552
Csp6I	GTAC	5 cut(s)	581, 1430, 1516, 2254, 2566
CviQI	GTAC	5 cut(s)	581, 1430, 1516, 2254, 2566
DdeI	CTNAG	6 cut(s)	84, 360, 374, 1316, 2247, 2511
DinI	GGCGCC	2 cut(s)	75, 851
DraI	TTTAAA	1 cut(s)	1573
DrdI	GACNNNNNNGTC	1 cut(s)	1640
DriI	GACNNNNNGTC	1 cut(s)	244
DseDI	GACNNNNNNGTC	1 cut(s)	1640
EaeI	YGGCCR	1 cut(s)	1176
Eam1104I	CTCTTC	3 cut(s)	300, 354, 1819
Eam1105I	GACNNNNNGTC	1 cut(s)	244
EarI	CTCTTC	3 cut(s)	300, 354, 1819
Eco130I	CCWWGG	2 cut(s)	919, 1636
Eco147I	AGGCCT	1 cut(s)	720
Eco31I	GGTCTC	1 cut(s)	346
Eco32I	GATATC	1 cut(s)	1354
Eco47I	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
Eco57I	CTGAAG	7 cut(s)	309, 651, 758, 1179, 1671, 1886, 2130
Eco72I	CACGTG	3 cut(s)	206, 236, 2132
EcoNI	CCTNNNNNAGG	1 cut(s)	2444
EcoO109I	RGGNCCY	2 cut(s)	1504, 1642
EcoRI	GAATTC	1 cut(s)	948
EcoRII	CCWGG	5 cut(s)	473, 1390, 1552, 2342, 2368
EcoRV	GATATC	1 cut(s)	1354
EcoT14I	CCWWGG	2 cut(s)	919, 1636
EgeI	GGCGCC	2 cut(s)	75, 851
EheI	GGCGCC	2 cut(s)	75, 851
ErhI	CCWWGG	2 cut(s)	919, 1636
FaqI	GGGAC	2 cut(s)	225, 2386
FauI	CCCGC	2 cut(s)	691, 1887
FauNDI	CATATG	2 cut(s)	1249, 1522
FbaI	TGATCA	1 cut(s)	1837
FokI	GGATG	8 cut(s)	514, 587, 838, 888, 1457, 1526, 1910, 2156
FspBI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2520
GlaI	GCGC	2 cut(s)	75, 851
GsaI	CCCAGC	1 cut(s)	2028
GsuI	CTGGAG	1 cut(s)	1602
HaeII	RGCGCY	2 cut(s)	77, 853
HaeIII	GGCC	6 cut(s)	720, 1178, 1239, 2166, 2313, 2554
HapII	CCGG	3 cut(s)	905, 1935, 2581
HgaI	GACGC	1 cut(s)	1697
HhaI	GCGC	2 cut(s)	76, 852
Hin1I	GRCGYC	2 cut(s)	74, 850
Hin6I	GCGC	2 cut(s)	74, 850
HinP1I	GCGC	2 cut(s)	74, 850
HincII	GTYRAC	1 cut(s)	748
HindII	GTYRAC	1 cut(s)	748
HindIII	AAGCTT	2 cut(s)	56, 448
HinfI	GANTC	2 cut(s)	967, 2480
HpaI	GTTAAC	1 cut(s)	748
HpaII	CCGG	3 cut(s)	905, 1935, 2581
HphI	GGTGA	3 cut(s)	800, 1958, 2159
Hpy166II	GTNNAC	2 cut(s)	748, 1516
Hpy8I	GTNNAC	2 cut(s)	748, 1516
Hpy99I	CGWCG	2 cut(s)	10, 1020
HpyAV	CCTTC	7 cut(s)	87, 358, 452, 779, 1646, 1937, 2440
HpyCH4III	ACNGT	1 cut(s)	675
HpyCH4IV	ACGT	7 cut(s)	205, 235, 433, 779, 1741, 1833, 2131
HpyF3I	CTNAG	6 cut(s)	84, 360, 374, 1316, 2247, 2511
HpySE526I	ACGT	7 cut(s)	205, 235, 433, 779, 1741, 1833, 2131
Hsp92I	GRCGYC	2 cut(s)	74, 850
HspAI	GCGC	2 cut(s)	74, 850
KasI	GGCGCC	2 cut(s)	73, 849
KpnI	GGTACC	1 cut(s)	1433
Ksp22I	TGATCA	1 cut(s)	1837
KspAI	GTTAAC	1 cut(s)	748
LguI	GCTCTTC	1 cut(s)	354
LmnI	GCTCC	9 cut(s)	10, 45, 172, 574, 1135, 1397, 1878, 2066, 2583
MabI	ACCWGGT	1 cut(s)	1552
MaeI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2520
MaeII	ACGT	7 cut(s)	205, 235, 433, 779, 1741, 1833, 2131
MaeIII	GTNAC	3 cut(s)	236, 605, 1829
MbiI	CCGCTC	1 cut(s)	83
MfeI	CAATTG	1 cut(s)	1620
MflI	RGATCY	5 cut(s)	1050, 1091, 1321, 1727, 2187
MhlI	GDGCHC	1 cut(s)	2125
Mly113I	GGCGCC	2 cut(s)	74, 850
MmeI	TCCRAC	2 cut(s)	349, 913
MslI	CAYNNNNRTG	2 cut(s)	1412, 2011
MspA1I	CMGCKG	1 cut(s)	1376
MspCI	CTTAAG	2 cut(s)	242, 451
MspI	CCGG	3 cut(s)	905, 1935, 2581
MspR9I	CCNGG	6 cut(s)	475, 1392, 1554, 2344, 2370, 2581
MunI	CAATTG	1 cut(s)	1620
Mva1269I	GAATGC	1 cut(s)	2069
MvaI	CCWGG	5 cut(s)	475, 1392, 1554, 2344, 2370
NarI	GGCGCC	2 cut(s)	74, 850
NciI	CCSGG	1 cut(s)	2581
NcoI	CCATGG	1 cut(s)	1636
NdeI	CATATG	2 cut(s)	1249, 1522
NheI	GCTAGC	2 cut(s)	1627, 2519
NlaIV	GGNNCC	9 cut(s)	75, 168, 767, 851, 1170, 1431, 1506, 1643, 1933
NmeAIII	GCCGAG	2 cut(s)	323, 1265
NmuCI	GTSAC	2 cut(s)	236, 605
NspI	RCATGY	3 cut(s)	397, 754, 2420
PceI	AGGCCT	1 cut(s)	720
PciI	ACATGT	1 cut(s)	393
PciSI	GCTCTTC	1 cut(s)	354
PcsI	WCGNNNNNNNCGW	1 cut(s)	1237
PctI	GAATGC	1 cut(s)	2069
PfeI	GAWTC	2 cut(s)	967, 2480
PflMI	CCANNNNNTGG	2 cut(s)	331, 2299
PluTI	GGCGCC	2 cut(s)	77, 853
PmaCI	CACGTG	3 cut(s)	206, 236, 2132
PmlI	CACGTG	3 cut(s)	206, 236, 2132
Ppu21I	YACGTR	3 cut(s)	206, 236, 2132
PpuMI	RGGWCCY	2 cut(s)	1504, 1642
PscI	ACATGT	1 cut(s)	393
PshBI	ATTAAT	1 cut(s)	1152
Psp1406I	AACGTT	1 cut(s)	1833
Psp5II	RGGWCCY	2 cut(s)	1504, 1642
Psp6I	CCWGG	5 cut(s)	473, 1390, 1552, 2342, 2368
PspCI	CACGTG	3 cut(s)	206, 236, 2132
PspFI	CCCAGC	1 cut(s)	2024
PspGI	CCWGG	5 cut(s)	473, 1390, 1552, 2342, 2368
PspN4I	GGNNCC	9 cut(s)	75, 168, 767, 851, 1170, 1431, 1506, 1643, 1933
PspPI	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
PspPPI	RGGWCCY	2 cut(s)	1504, 1642
PstI	CTGCAG	1 cut(s)	318
PstNI	CAGNNNCTG	1 cut(s)	1190
PsuI	RGATCY	5 cut(s)	1050, 1091, 1321, 1727, 2187
PvuII	CAGCTG	1 cut(s)	1376
RsaI	GTAC	5 cut(s)	582, 1431, 1517, 2255, 2567
RsaNI	GTAC	5 cut(s)	581, 1430, 1516, 2254, 2566
RseI	CAYNNNNRTG	2 cut(s)	1412, 2011
SapI	GCTCTTC	1 cut(s)	354
Sau96I	GGNCC	6 cut(s)	30, 322, 477, 1237, 1504, 1642
ScrFI	CCNGG	6 cut(s)	475, 1392, 1554, 2344, 2370, 2581
SduI	GDGCHC	1 cut(s)	2125
SexAI	ACCWGGT	1 cut(s)	1552
SfcI	CTRYAG	4 cut(s)	98, 314, 1344, 2043
SfoI	GGCGCC	2 cut(s)	75, 851
SinI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
SmiMI	CAYNNNNRTG	2 cut(s)	1412, 2011
SmlI	CTYRAG	8 cut(s)	140, 242, 451, 546, 1063, 2226, 2273, 2503
SmoI	CTYRAG	8 cut(s)	140, 242, 451, 546, 1063, 2226, 2273, 2503
SpeI	ACTAGT	1 cut(s)	494
SseBI	AGGCCT	1 cut(s)	720
SsiI	CCGC	9 cut(s)	71, 81, 95, 501, 684, 1175, 1793, 1894, 2414
SspDI	GGCGCC	2 cut(s)	73, 849
SspI	AATATT	2 cut(s)	1772, 2207
SspMI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2520
StuI	AGGCCT	1 cut(s)	720
StyD4I	CCNGG	6 cut(s)	473, 1390, 1552, 2342, 2368, 2579
StyI	CCWWGG	2 cut(s)	919, 1636
TaaI	ACNGT	1 cut(s)	675
TaiI	ACGT	7 cut(s)	208, 238, 436, 782, 1744, 1836, 2134
TaqI	TCGA	6 cut(s)	63, 464, 1305, 1957, 2075, 2155
TaqII	GACCGA	1 cut(s)	47
TatI	WGTACW	2 cut(s)	580, 2253
TauI	GCSGC	2 cut(s)	74, 1178
TfiI	GAWTC	2 cut(s)	967, 2480
TseFI	GTSAC	2 cut(s)	236, 605
Tsp45I	GTSAC	2 cut(s)	236, 605
TspDTI	ATGAA	7 cut(s)	543, 885, 941, 1098, 1430, 2049, 2515
TspGWI	ACGGA	5 cut(s)	23, 169, 273, 593, 1157
Van91I	CCANNNNNTGG	2 cut(s)	331, 2299
Vha464I	CTTAAG	2 cut(s)	242, 451
VpaK11BI	GGWCC	5 cut(s)	30, 322, 477, 1504, 1642
VspI	ATTAAT	1 cut(s)	1152
XagI	CCTNNNNNAGG	1 cut(s)	2444
XapI	RAATTY	7 cut(s)	106, 937, 948, 1469, 1855, 2049, 2404
XbaI	TCTAGA	1 cut(s)	991
XceI	RCATGY	3 cut(s)	397, 754, 2420
XspI	CTAG	8 cut(s)	495, 540, 567, 857, 992, 1280, 1628, 2520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.