pycom14g07000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0009266
Physical Location & Seq
Forward (+)
6957817 .. 6960085
2269 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 564 bp
ATGGCAACCACCTCTCGCTCATTGTACCGCACACTGTGCCTGCGGTCCTTCTCCACAAACACAAAGCCAACGCATCACAACAGCCACCAGCAGAACCACCAGTACACGGAACCCAATTCCTTCATTGGGTGTTGGGAGGCCCCCAAGGACCCCAAGGAGGCGGAAGCCAAGCTTGCACAGCTCCGCCGAGACTACGCCAAGCAGGTCAAGGAGGTCCGCAAGGAGTACATCAAGGAGGTTGAGCTCATGAGGCTTGATAAGCTTCGCAAGGACGAGGCTCGTAAAGAAGCCCTTAGGCTCCAGAACGAGGAGCGCAAGAGGCTCAAGGCAGAGGCTGCAAAGGTCCGTGCCCAACAGCGCGAGGTTGCCGAGCAGGAGTTCCGGCAAACGCTGTTGAAAGAAAGAGCAGAGAAGCTTGAGAATTGGAAGATGAAGGAAAAACTGAAAAAAGAGATGAAAAAAGACGAGAAAGAACTTCTGCGACACAAGAGTTCCATGTGGATCGATGAACAAGAACTGGAAAAGAAGATTTTAGAAGCCATCGTTGATGCCACACCCCTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

188

Amino Acids

22.43

Weight (kDa)

9.5

Isoelectric Point (pI)

41.08

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 193
AccII CGCG 1 cut(s) 360
AciI CCGC 5 cut(s) 28, 43, 161, 184, 217
AclWI GGATC 1 cut(s) 509
AdeI CACNNNGTG 1 cut(s) 36
AfaI GTAC 3 cut(s) 26, 104, 227
AfiI CCNNNNNNNGG 4 cut(s) 106, 126, 157, 307
AgsI TTSAA 1 cut(s) 397
AluBI AGCT 5 cut(s) 172, 181, 244, 262, 415
AluI AGCT 5 cut(s) 172, 181, 244, 262, 415
Alw21I GWGCWC 1 cut(s) 246
Alw26I GTCTC 1 cut(s) 183
AlwI GGATC 1 cut(s) 509
AlwNI CAGNNNCTG 1 cut(s) 335
AoxI GGCC 1 cut(s) 138
ApeKI GCWGC 1 cut(s) 335
AspLEI GCGC 2 cut(s) 315, 360
AspS9I GGNCC 5 cut(s) 45, 139, 148, 214, 343
AvaII GGWCC 4 cut(s) 45, 148, 214, 343
AxyI CCTNAGG 1 cut(s) 293
BaeGI GKGCMC 1 cut(s) 352
BanII GRGCYC 1 cut(s) 246
Bbv12I GWGCWC 1 cut(s) 246
BbvI GCAGC 1 cut(s) 322
BccI CCATC 1 cut(s) 548
BcoDI GTCTC 1 cut(s) 183
BfuAI ACCTGC 1 cut(s) 193
BisI GCNGC 1 cut(s) 336
BlsI GCNGC 1 cut(s) 337
Bme18I GGWCC 4 cut(s) 45, 148, 214, 343
BmgT120I GGNCC 5 cut(s) 45, 139, 148, 214, 343
BmiI GGNNCC 4 cut(s) 111, 141, 150, 299
BmsI GCATC 2 cut(s) 82, 538
BpmI CTGGAG 1 cut(s) 284
BpuEI CTTGAG 2 cut(s) 308, 437
Bsa29I ATCGAT 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 144, 153
Bsc4I CCNNNNNNNGG 4 cut(s) 106, 126, 157, 307
Bse1I ACTGG 2 cut(s) 100, 522
Bse21I CCTNAGG 1 cut(s) 293
BseCI ATCGAT 1 cut(s) 504
BseDI CCNNGG 2 cut(s) 144, 153
BseLI CCNNNNNNNGG 4 cut(s) 106, 126, 157, 307
BseNI ACTGG 2 cut(s) 100, 522
BseRI GAGGAG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 352
BseXI GCAGC 1 cut(s) 322
Bsh1236I CGCG 1 cut(s) 360
BshFI GGCC 1 cut(s) 140
BshVI ATCGAT 1 cut(s) 504
BsiHKAI GWGCWC 1 cut(s) 246
BsiSI CCGG 1 cut(s) 382
BslI CCNNNNNNNGG 4 cut(s) 106, 126, 157, 307
BsmAI GTCTC 1 cut(s) 183
BsnI GGCC 1 cut(s) 140
Bsp1286I GDGCHC 2 cut(s) 246, 352
Bsp143I GATC 1 cut(s) 501
BspACI CCGC 5 cut(s) 28, 43, 161, 184, 217
BspANI GGCC 1 cut(s) 140
BspDI ATCGAT 1 cut(s) 504
BspFNI CGCG 1 cut(s) 360
BspHI TCATGA 1 cut(s) 246
BspLI GGNNCC 4 cut(s) 111, 141, 150, 299
BspMI ACCTGC 1 cut(s) 193
BspPI GGATC 1 cut(s) 509
BsrI ACTGG 2 cut(s) 100, 522
BssECI CCNNGG 2 cut(s) 144, 153
BssMI GATC 1 cut(s) 501
BssT1I CCWWGG 2 cut(s) 144, 153
Bst4CI ACNGT 1 cut(s) 36
BstAPI GCANNNNNTGC 2 cut(s) 36, 335
BstC8I GCNNGC 2 cut(s) 41, 174
BstDEI CTNAG 1 cut(s) 293
BstFNI CGCG 1 cut(s) 360
BstHHI GCGC 2 cut(s) 315, 360
BstKTI GATC 1 cut(s) 504
BstMAI GTCTC 1 cut(s) 183
BstMBI GATC 1 cut(s) 501
BstMWI GCNNNNNNNGC 7 cut(s) 36, 173, 178, 250, 259, 319, 335
BstSLI GKGCMC 1 cut(s) 352
BstUI CGCG 1 cut(s) 360
BstV1I GCAGC 1 cut(s) 322
Bsu15I ATCGAT 1 cut(s) 504
Bsu36I CCTNAGG 1 cut(s) 293
BsuRI GGCC 1 cut(s) 140
BsuTUI ATCGAT 1 cut(s) 504
BtsIMutI CAGTG 1 cut(s) 32
BveI ACCTGC 1 cut(s) 193
Cac8I GCNNGC 2 cut(s) 41, 174
CaiI CAGNNNCTG 1 cut(s) 335
CciI TCATGA 1 cut(s) 246
CfoI GCGC 2 cut(s) 315, 360
Cfr13I GGNCC 5 cut(s) 45, 139, 148, 214, 343
ClaI ATCGAT 1 cut(s) 504
Csp6I GTAC 3 cut(s) 25, 103, 226
CviAII CATG 2 cut(s) 247, 496
CviQI GTAC 3 cut(s) 25, 103, 226
DdeI CTNAG 1 cut(s) 293
DpnI GATC 1 cut(s) 503
DpnII GATC 1 cut(s) 501
DraIII CACNNNGTG 1 cut(s) 36
EciI GGCGGA 2 cut(s) 173, 176
Ecl136II GAGCTC 1 cut(s) 244
Eco130I CCWWGG 2 cut(s) 144, 153
Eco24I GRGCYC 1 cut(s) 246
Eco47I GGWCC 4 cut(s) 45, 148, 214, 343
Eco53kI GAGCTC 1 cut(s) 244
Eco81I CCTNAGG 1 cut(s) 293
EcoICRI GAGCTC 1 cut(s) 244
EcoO109I RGGNCCY 2 cut(s) 139, 148
EcoT14I CCWWGG 2 cut(s) 144, 153
EcoT38I GRGCYC 1 cut(s) 246
ErhI CCWWGG 2 cut(s) 144, 153
FaeI CATG 2 cut(s) 250, 499
FaiI YATR 2 cut(s) 248, 497
FalI AAGNNNNNCTT 4 cut(s) 156, 188, 276, 308
FatI CATG 2 cut(s) 246, 495
Fnu4HI GCNGC 1 cut(s) 336
FriOI GRGCYC 1 cut(s) 246
Fsp4HI GCNGC 1 cut(s) 336
GlaI GCGC 2 cut(s) 314, 359
GluI GCNGC 1 cut(s) 336
GsuI CTGGAG 1 cut(s) 284
HaeIII GGCC 1 cut(s) 140
HapII CCGG 1 cut(s) 382
HhaI GCGC 2 cut(s) 315, 360
Hin1II CATG 2 cut(s) 250, 499
Hin6I GCGC 2 cut(s) 313, 358
HinP1I GCGC 2 cut(s) 313, 358
HindIII AAGCTT 3 cut(s) 170, 260, 413
HpaII CCGG 1 cut(s) 382
Hpy166II GTNNAC 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 247, 301
Hpy8I GTNNAC 1 cut(s) 105
HpyAV CCTTC 3 cut(s) 58, 130, 427
HpyCH4III ACNGT 1 cut(s) 36
HpyCH4V TGCA 2 cut(s) 176, 338
HpyF10VI GCNNNNNNNGC 7 cut(s) 36, 173, 178, 250, 259, 319, 335
HpyF3I CTNAG 1 cut(s) 293
Hsp92II CATG 2 cut(s) 250, 499
HspAI GCGC 2 cut(s) 313, 358
Kzo9I GATC 1 cut(s) 501
LmnI GCTCC 3 cut(s) 186, 303, 310
LpnPI CCDG 8 cut(s) 53, 101, 113, 188, 314, 359, 395, 503
Lsp1109I GCAGC 1 cut(s) 322
LweI GCATC 2 cut(s) 82, 538
MalI GATC 1 cut(s) 503
MboI GATC 1 cut(s) 501
MboII GAAGA 2 cut(s) 439, 538
MhlI GDGCHC 2 cut(s) 246, 352
MluCI AATT 2 cut(s) 115, 421
MspI CCGG 1 cut(s) 382
MvnI CGCG 1 cut(s) 360
MwoI GCNNNNNNNGC 7 cut(s) 36, 173, 178, 250, 259, 319, 335
NdeII GATC 1 cut(s) 501
NlaIII CATG 2 cut(s) 250, 499
NlaIV GGNNCC 4 cut(s) 111, 141, 150, 299
NmeAIII GCCGAG 2 cut(s) 212, 394
PagI TCATGA 1 cut(s) 246
PkrI GCNGC 1 cut(s) 337
PpuMI RGGWCCY 1 cut(s) 148
Psp124BI GAGCTC 1 cut(s) 246
Psp5II RGGWCCY 1 cut(s) 148
PspN4I GGNNCC 4 cut(s) 111, 141, 150, 299
PspPI GGNCC 5 cut(s) 45, 139, 148, 214, 343
PspPPI RGGWCCY 1 cut(s) 148
PsrI GAACNNNNNNTAC 2 cut(s) 86, 118
PstNI CAGNNNCTG 1 cut(s) 335
RsaI GTAC 3 cut(s) 26, 104, 227
RsaNI GTAC 3 cut(s) 25, 103, 226
SacI GAGCTC 1 cut(s) 246
SatI GCNGC 1 cut(s) 336
Sau3AI GATC 1 cut(s) 501
Sau96I GGNCC 5 cut(s) 45, 139, 148, 214, 343
SduI GDGCHC 2 cut(s) 246, 352
SfaNI GCATC 2 cut(s) 82, 538
SinI GGWCC 4 cut(s) 45, 148, 214, 343
SmlI CTYRAG 2 cut(s) 323, 416
SmoI CTYRAG 2 cut(s) 323, 416
Sse9I AATT 2 cut(s) 115, 421
SsiI CCGC 5 cut(s) 28, 43, 161, 184, 217
SstI GAGCTC 1 cut(s) 246
StyI CCWWGG 2 cut(s) 144, 153
TaaI ACNGT 1 cut(s) 36
TaqI TCGA 1 cut(s) 504
TasI AATT 2 cut(s) 115, 421
TatI WGTACW 2 cut(s) 102, 225
TscAI CASTG 1 cut(s) 39
TseI GCWGC 1 cut(s) 335
TspDTI ATGAA 4 cut(s) 112, 446, 470, 522
TspGWI ACGGA 2 cut(s) 122, 335
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 4 cut(s) 45, 148, 214, 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.