pycom14g10500

Belongs to the glycosyl hydrolase 28 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008948
Physical Location & Seq
Forward (+)
13202349 .. 13205434
3086 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1617 bp
ATGATCTCCATCTATTTTGCTCTGCAGATCAAACTAAAAACAATAGAAAATAAATACAGTACACAACAAACTGCAGACTCTATATATACACATACTTCATTTCGCAAGAAACTAAACTACAGACGACAAAGTTTGAATAACCATCATTTTTTTTGTACCATGAGGAGGAGGACTTCCATGAATACTCAGTTATCTGAGATGTTGATGGCAGTAGTATTTGTTGTTTTATTGGCCACAACAAAAGGAGTTGAAAGCCGCAAGGCTCGGAGGGAGAATGCAGGAGGAGAATACTTCGAGTACTGCGCCATAAGTTGCAGAGCCAAAAGTGCTTCTCTGACAGATTATGGAGGAGTTGGCGATGGAACGACATCAAACACTAAGGCTTTTCAAGAAGCAATCACTCATCTGAGTCAGTACGAGTCAGAAGGCGGGTCACAACTCGTTGTCCCTCCTGGTAAATGGTTAACTGGCAGCTTCAACCTCACCAGCCATTTCACGCTTTATCTCCATAAGGATGCAGTTCTTCTTGCTTCTCAGGACGAGAGCGAATGGCCGGTGATTGAACCCCTTCCGTCGTATGGCCGAGGAAGGGACAACACAGATGGTGGAAGATTCATCAGTCTGATATTTGGAACCAATCTCACTGACGTTGTAATAACAGGTGACAATGGCACCATCGATGGCCAAGGTGAAGTATGGTGGCAGAAATTCAAAAAGGGACAGTTAAATTACACGCGACCGTACCTGATCGAAATCATGTACTCCGAAAAAATCCAGATTTCCAACCTCACCTTGGTTAATTCTCCTTCATGGAATGTTCATCCCATTTACAGCAGCAATGTTCTTGTTCAAGGCATTACAATCCTTGCACCAGTGACATCTCCAAACACAGATGGGATCAACCCCGATTCTTGCACAAATACTCGAATTGAGGACTGCTACATTGTCTCTGGGGATGATTGCATAGCAGTAAAGAGCGGTTGGGATGAGTATGGAATTGCTTTCGGGATGCCTACGAAACAGCTTGTGATCAGACGGTTGACATGCATTTCTCCATTCAGTGCAGTGATTGCACTTGGGAGCGAGATGTCAGGTGGAATCCAAGATGTGAGGGCGGAGGATATTTTTGCCATTGACTCGGAATCAGGAGTTCGAATCAAAACTGCTGTGGGAAGAGGAGGGTTTGTGAAAGACATATACGTGAGAGGAATGACTATGAAAACAATGAAATGGGCGTTTTGGATGACAGGAAACTACGGATCCCATGCAGATAATGGCTACGATCCGAATGCCCTTCCTGTGATTCAGAATATAAATTATCATGACATGGTTGCTGAGAATGTGACAATGGCAGCTAAGTTGGAGGGGATTCCTGGCGATCCGTTTACAGGGATTTGCATATCGAACGTCACGATTACACTGGCAAAGAAGGCGAAGAAATTACCATGGAATTGCACTGATATTGCCGGGATTTCAAGCGGCGTGGTTCCTCAGCCATGTCAGGCTCTGGCAGACCAGGGTCCAGGCAATGTTACAGCATGCAATTTTCCAGAAGAGAGCCTGCCAATTGATAATGTTCAAGTTCAGGTGTGCTCTTACAGGAGGGAGCACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glyco_hydro_28 Pectate_lyase_3

Protein Analysis

539

Amino Acids

59.46

Weight (kDa)

6.39

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_28 PF00295 207 - 480 4.8e-37 Glycosyl hydrolases family 28
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 671
AccBSI CCGCTC 1 cut(s) 978
AccII CGCG 1 cut(s) 736
AciI CCGC 5 cut(s) 256, 429, 978, 1115, 1479
AclWI GGATC 5 cut(s) 905, 1254, 1267, 1277, 1373
AcoI YGGCCR 4 cut(s) 231, 551, 580, 682
AcsI RAATTY 1 cut(s) 707
AfaI GTAC 6 cut(s) 61, 157, 299, 416, 743, 761
AfiI CCNNNNNNNGG 5 cut(s) 165, 578, 589, 793, 1388
AgsI TTSAA 9 cut(s) 136, 251, 389, 478, 563, 712, 851, 1476, 1580
AjnI CCWGG 4 cut(s) 451, 1372, 1515, 1522
AluBI AGCT 3 cut(s) 474, 1024, 1355
AluI AGCT 3 cut(s) 474, 1024, 1355
Alw21I GWGCWC 2 cut(s) 1595, 1611
Alw26I GTCTC 1 cut(s) 952
AlwI GGATC 5 cut(s) 905, 1254, 1267, 1277, 1373
AlwNI CAGNNNCTG 1 cut(s) 1507
AoxI GGCC 4 cut(s) 231, 551, 580, 682
ApeKI GCWGC 3 cut(s) 471, 834, 1352
ApoI RAATTY 1 cut(s) 707
Asp700I GAANNNNTTC 1 cut(s) 567
AspLEI GCGC 1 cut(s) 305
AspS9I GGNCC 1 cut(s) 1520
AsuC2I CCSGG 1 cut(s) 1468
AsuHPI GGTGA 5 cut(s) 475, 568, 674, 701, 781
AsuII TTCGAA 1 cut(s) 1153
AvaII GGWCC 1 cut(s) 1520
BalI TGGCCA 2 cut(s) 233, 684
BamHI GGATCC 1 cut(s) 1259
BanI GGYRCC 1 cut(s) 671
Bbv12I GWGCWC 2 cut(s) 1595, 1611
BbvCI CCTCAGC 1 cut(s) 1491
BbvI GCAGC 3 cut(s) 483, 846, 1364
BccI CCATC 8 cut(s) 17, 150, 199, 353, 596, 674, 683, 887
BcgI CGANNNNNNTGC 2 cut(s) 1271, 1305
BciT130I CCWGG 4 cut(s) 453, 1374, 1517, 1524
BclI TGATCA 1 cut(s) 1029
BcnI CCSGG 1 cut(s) 1468
BcoDI GTCTC 1 cut(s) 952
BfmI CTRYAG 3 cut(s) 23, 72, 118
BisI GCNGC 5 cut(s) 256, 472, 835, 1353, 1480
BlsI GCNGC 5 cut(s) 257, 473, 836, 1354, 1481
BmcAI AGTACT 1 cut(s) 299
Bme1390I CCNGG 5 cut(s) 453, 1374, 1468, 1517, 1524
Bme18I GGWCC 1 cut(s) 1520
BmgT120I GGNCC 1 cut(s) 1520
BmiI GGNNCC 5 cut(s) 634, 673, 1261, 1488, 1521
BmrFI CCNGG 5 cut(s) 453, 1374, 1468, 1517, 1524
BmsI GCATC 2 cut(s) 505, 999
BoxI GACNNNNGTC 1 cut(s) 1518
Bpu10I CCTNAGC 1 cut(s) 1491
Bpu14I TTCGAA 1 cut(s) 1153
BpuMI CCSGG 1 cut(s) 1468
Bsa29I ATCGAT 1 cut(s) 678
BsaAI YACGTR 1 cut(s) 1201
BsaBI GATNNNNATC 2 cut(s) 8, 752
BsaJI CCNNGG 5 cut(s) 583, 685, 792, 1445, 1516
BsaXI ACNNNNNCTCC 3 cut(s) 1170, 1200, 1598
Bsc4I CCNNNNNNNGG 5 cut(s) 165, 578, 589, 793, 1388
Bse118I RCCGGY 1 cut(s) 553
Bse1I ACTGG 4 cut(s) 472, 872, 1425, 1616
Bse3DI GCAATG 2 cut(s) 844, 1534
Bse8I GATNNNNATC 2 cut(s) 8, 752
BseBI CCWGG 4 cut(s) 453, 1374, 1517, 1524
BseCI ATCGAT 1 cut(s) 678
BseDI CCNNGG 5 cut(s) 583, 685, 792, 1445, 1516
BseGI GGATG 6 cut(s) 520, 820, 961, 991, 1014, 1248
BseJI GATNNNNATC 2 cut(s) 8, 752
BseLI CCNNNNNNNGG 5 cut(s) 165, 578, 589, 793, 1388
BseMI GCAATG 2 cut(s) 844, 1534
BseMII CTCAG 6 cut(s) 186, 200, 398, 548, 1326, 1505
BseNI ACTGG 4 cut(s) 472, 872, 1425, 1616
BseRI GAGGAG 5 cut(s) 178, 181, 297, 363, 1191
BseXI GCAGC 3 cut(s) 483, 846, 1364
BsgI GTGCAG 1 cut(s) 1083
Bsh1236I CGCG 1 cut(s) 736
Bsh1285I CGRYCG 1 cut(s) 740
BshFI GGCC 4 cut(s) 233, 553, 582, 684
BshNI GGYRCC 1 cut(s) 671
BshVI ATCGAT 1 cut(s) 678
BsiEI CGRYCG 1 cut(s) 740
BsiHKAI GWGCWC 2 cut(s) 1595, 1611
BsiSI CCGG 2 cut(s) 554, 1467
BslFI GGGAC 3 cut(s) 431, 605, 732
BslI CCNNNNNNNGG 5 cut(s) 165, 578, 589, 793, 1388
BsmAI GTCTC 1 cut(s) 952
BsmFI GGGAC 3 cut(s) 431, 605, 732
BsmI GAATGC 2 cut(s) 280, 1294
BsnI GGCC 4 cut(s) 233, 553, 582, 684
Bsp119I TTCGAA 1 cut(s) 1153
Bsp1286I GDGCHC 2 cut(s) 1595, 1611
Bsp143I GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
Bsp19I CCATGG 1 cut(s) 1445
BspACI CCGC 5 cut(s) 256, 429, 978, 1115, 1479
BspANI GGCC 4 cut(s) 233, 553, 582, 684
BspCNI CTCAG 6 cut(s) 187, 199, 399, 547, 1327, 1504
BspDI ATCGAT 1 cut(s) 678
BspFNI CGCG 1 cut(s) 736
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 5 cut(s) 634, 673, 1261, 1488, 1521
BspMAI CTGCAG 2 cut(s) 27, 76
BspPI GGATC 5 cut(s) 905, 1254, 1267, 1277, 1373
BspT104I TTCGAA 1 cut(s) 1153
BspT107I GGYRCC 1 cut(s) 671
BsrBI CCGCTC 1 cut(s) 978
BsrDI GCAATG 2 cut(s) 844, 1534
BsrFI RCCGGY 1 cut(s) 553
BsrI ACTGG 4 cut(s) 472, 872, 1425, 1616
BssAI RCCGGY 1 cut(s) 553
BssECI CCNNGG 5 cut(s) 583, 685, 792, 1445, 1516
BssMI GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
BssT1I CCWWGG 3 cut(s) 685, 792, 1445
Bst2UI CCWGG 4 cut(s) 453, 1374, 1517, 1524
Bst4CI ACNGT 4 cut(s) 59, 723, 741, 1038
Bst6I CTCTTC 2 cut(s) 1168, 1548
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstBAI YACGTR 1 cut(s) 1201
BstBI TTCGAA 1 cut(s) 1153
BstC8I GCNNGC 2 cut(s) 1540, 1562
BstDEI CTNAG 8 cut(s) 186, 195, 378, 407, 534, 1335, 1356, 1491
BstDSI CCRYGG 1 cut(s) 1445
BstF5I GGATG 6 cut(s) 520, 820, 961, 991, 1014, 1248
BstFNI CGCG 1 cut(s) 736
BstHHI GCGC 1 cut(s) 305
BstKTI GATC 8 cut(s) 6, 30, 750, 900, 1032, 1262, 1285, 1381
BstMAI GTCTC 1 cut(s) 952
BstMBI GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
BstMCI CGRYCG 1 cut(s) 740
BstMWI GCNNNNNNNGC 3 cut(s) 326, 1070, 1430
BstNI CCWGG 4 cut(s) 453, 1374, 1517, 1524
BstNSI RCATGY 2 cut(s) 1047, 1542
BstPAI GACNNNNGTC 1 cut(s) 1518
BstSCI CCNGG 5 cut(s) 451, 1372, 1466, 1515, 1522
BstSFI CTRYAG 3 cut(s) 23, 72, 118
BstUI CGCG 1 cut(s) 736
BstV1I GCAGC 3 cut(s) 483, 846, 1364
BstX2I RGATCY 1 cut(s) 1259
BstYI RGATCY 1 cut(s) 1259
Bsu15I ATCGAT 1 cut(s) 678
BsuRI GGCC 4 cut(s) 233, 553, 582, 684
BsuTUI ATCGAT 1 cut(s) 678
BtgI CCRYGG 1 cut(s) 1445
BtgZI GCGATG 1 cut(s) 372
BtsCI GGATG 6 cut(s) 520, 820, 961, 991, 1014, 1248
BtsI GCAGTG 1 cut(s) 1071
BtsIMutI CAGTG 7 cut(s) 642, 879, 1066, 1071, 1418, 1455, 1609
Cac8I GCNNGC 2 cut(s) 1540, 1562
CaiI CAGNNNCTG 1 cut(s) 1507
CciI TCATGA 1 cut(s) 1321
CfoI GCGC 1 cut(s) 305
Cfr10I RCCGGY 1 cut(s) 553
Cfr13I GGNCC 1 cut(s) 1520
ClaI ATCGAT 1 cut(s) 678
Csp6I GTAC 6 cut(s) 60, 156, 298, 415, 742, 760
CspCI CAANNNNNGTGG 2 cut(s) 1464, 1499
CviQI GTAC 6 cut(s) 60, 156, 298, 415, 742, 760
DdeI CTNAG 8 cut(s) 186, 195, 378, 407, 534, 1335, 1356, 1491
DpnI GATC 8 cut(s) 5, 29, 749, 899, 1031, 1261, 1284, 1380
DpnII GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
EaeI YGGCCR 4 cut(s) 231, 551, 580, 682
Eam1104I CTCTTC 2 cut(s) 1168, 1548
EarI CTCTTC 2 cut(s) 1168, 1548
EciI GGCGGA 1 cut(s) 1130
Eco130I CCWWGG 3 cut(s) 685, 792, 1445
Eco47I GGWCC 1 cut(s) 1520
EcoRII CCWGG 4 cut(s) 451, 1372, 1515, 1522
EcoT14I CCWWGG 3 cut(s) 685, 792, 1445
EcoT22I ATGCAT 1 cut(s) 1049
ErhI CCWWGG 3 cut(s) 685, 792, 1445
FaqI GGGAC 3 cut(s) 431, 605, 732
FauI CCCGC 1 cut(s) 422
FbaI TGATCA 1 cut(s) 1029
Fnu4HI GCNGC 5 cut(s) 256, 472, 835, 1353, 1480
FokI GGATG 6 cut(s) 527, 807, 968, 998, 1021, 1255
Fsp4HI GCNGC 5 cut(s) 256, 472, 835, 1353, 1480
GlaI GCGC 1 cut(s) 304
GluI GCNGC 5 cut(s) 256, 472, 835, 1353, 1480
HaeIII GGCC 4 cut(s) 233, 553, 582, 684
HapII CCGG 2 cut(s) 554, 1467
HhaI GCGC 1 cut(s) 305
Hin6I GCGC 1 cut(s) 303
HinP1I GCGC 1 cut(s) 303
HincII GTYRAC 2 cut(s) 465, 1041
HindII GTYRAC 2 cut(s) 465, 1041
HpaI GTTAAC 1 cut(s) 465
HpaII CCGG 2 cut(s) 554, 1467
HphI GGTGA 5 cut(s) 475, 568, 674, 701, 781
Hpy166II GTNNAC 4 cut(s) 62, 465, 1041, 1386
Hpy188III TCNNGA 8 cut(s) 389, 536, 775, 1006, 1146, 1322, 1411, 1550
Hpy8I GTNNAC 4 cut(s) 62, 465, 1041, 1386
Hpy99I CGWCG 1 cut(s) 577
HpyAV CCTTC 6 cut(s) 419, 578, 582, 816, 1304, 1423
HpyCH4III ACNGT 4 cut(s) 59, 723, 741, 1038
HpyCH4IV ACGT 3 cut(s) 648, 1200, 1407
HpyF10VI GCNNNNNNNGC 3 cut(s) 326, 1070, 1430
HpyF3I CTNAG 8 cut(s) 186, 195, 378, 407, 534, 1335, 1356, 1491
HpySE526I ACGT 3 cut(s) 648, 1200, 1407
HspAI GCGC 1 cut(s) 303
Ksp22I TGATCA 1 cut(s) 1029
KspAI GTTAAC 1 cut(s) 465
Kzo9I GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
LmnI GCTCC 2 cut(s) 1080, 1606
Lsp1109I GCAGC 3 cut(s) 483, 846, 1364
LweI GCATC 2 cut(s) 505, 999
MaeII ACGT 3 cut(s) 648, 1200, 1407
MaeIII GTNAC 6 cut(s) 432, 662, 874, 1342, 1408, 1531
MalI GATC 8 cut(s) 5, 29, 749, 899, 1031, 1261, 1284, 1380
MbiI CCGCTC 1 cut(s) 978
MboI GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
MboII GAAGA 5 cut(s) 515, 621, 1185, 1447, 1565
MfeI CAATTG 1 cut(s) 1566
MflI RGATCY 1 cut(s) 1259
MhlI GDGCHC 2 cut(s) 1595, 1611
MlsI TGGCCA 2 cut(s) 233, 684
MluNI TGGCCA 2 cut(s) 233, 684
MlyI GAGTC 4 cut(s) 71, 418, 428, 1130
MmeI TCCRAC 2 cut(s) 807, 1341
Mox20I TGGCCA 2 cut(s) 233, 684
Mph1103I ATGCAT 1 cut(s) 1049
MroXI GAANNNNTTC 1 cut(s) 567
MscI TGGCCA 2 cut(s) 233, 684
MseI TTAA 3 cut(s) 464, 725, 798
MslI CAYNNNNRTG 2 cut(s) 513, 1199
Msp20I TGGCCA 2 cut(s) 233, 684
MspI CCGG 2 cut(s) 554, 1467
MspR9I CCNGG 5 cut(s) 453, 1374, 1468, 1517, 1524
MunI CAATTG 1 cut(s) 1566
Mva1269I GAATGC 2 cut(s) 280, 1294
MvaI CCWGG 4 cut(s) 453, 1374, 1517, 1524
MvnI CGCG 1 cut(s) 736
MwoI GCNNNNNNNGC 3 cut(s) 326, 1070, 1430
NciI CCSGG 1 cut(s) 1468
NcoI CCATGG 1 cut(s) 1445
NdeII GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
NlaIV GGNNCC 5 cut(s) 634, 673, 1261, 1488, 1521
NmeAIII GCCGAG 1 cut(s) 608
NmuCI GTSAC 5 cut(s) 432, 662, 874, 1342, 1408
NsiI ATGCAT 1 cut(s) 1049
NspI RCATGY 2 cut(s) 1047, 1542
NspV TTCGAA 1 cut(s) 1153
PaeI GCATGC 1 cut(s) 1542
PagI TCATGA 1 cut(s) 1321
PcsI WCGNNNNNNNCGW 1 cut(s) 1409
PctI GAATGC 2 cut(s) 280, 1294
PdmI GAANNNNTTC 1 cut(s) 567
PfeI GAWTC 7 cut(s) 612, 908, 1098, 1142, 1155, 1303, 1369
PkrI GCNGC 5 cut(s) 257, 473, 836, 1354, 1481
PleI GAGTC 4 cut(s) 71, 417, 427, 1130
PpsI GAGTC 4 cut(s) 71, 417, 427, 1130
Ppu21I YACGTR 1 cut(s) 1201
PshAI GACNNNNGTC 1 cut(s) 1518
Psp6I CCWGG 4 cut(s) 451, 1372, 1515, 1522
PspGI CCWGG 4 cut(s) 451, 1372, 1515, 1522
PspN4I GGNNCC 5 cut(s) 634, 673, 1261, 1488, 1521
PspPI GGNCC 1 cut(s) 1520
PstI CTGCAG 2 cut(s) 27, 76
PstNI CAGNNNCTG 1 cut(s) 1507
PsuI RGATCY 1 cut(s) 1259
RsaI GTAC 6 cut(s) 61, 157, 299, 416, 743, 761
RsaNI GTAC 6 cut(s) 60, 156, 298, 415, 742, 760
RseI CAYNNNNRTG 2 cut(s) 513, 1199
SaqAI TTAA 3 cut(s) 464, 725, 798
SatI GCNGC 5 cut(s) 256, 472, 835, 1353, 1480
Sau3AI GATC 8 cut(s) 3, 27, 747, 897, 1029, 1259, 1282, 1378
Sau96I GGNCC 1 cut(s) 1520
ScaI AGTACT 1 cut(s) 299
SchI GAGTC 4 cut(s) 71, 418, 428, 1130
ScrFI CCNGG 5 cut(s) 453, 1374, 1468, 1517, 1524
SduI GDGCHC 2 cut(s) 1595, 1611
SfaNI GCATC 2 cut(s) 505, 999
SfcI CTRYAG 3 cut(s) 23, 72, 118
SfuI TTCGAA 1 cut(s) 1153
SinI GGWCC 1 cut(s) 1520
SmiMI CAYNNNNRTG 2 cut(s) 513, 1199
SphI GCATGC 1 cut(s) 1542
SsiI CCGC 5 cut(s) 256, 429, 978, 1115, 1479
StyD4I CCNGG 5 cut(s) 451, 1372, 1466, 1515, 1522
StyI CCWWGG 3 cut(s) 685, 792, 1445
TaaI ACNGT 4 cut(s) 59, 723, 741, 1038
TaiI ACGT 3 cut(s) 651, 1203, 1410
TaqI TCGA 6 cut(s) 294, 678, 750, 925, 1153, 1403
TatI WGTACW 3 cut(s) 59, 297, 759
TauI GCSGC 2 cut(s) 258, 1482
TfiI GAWTC 7 cut(s) 612, 908, 1098, 1142, 1155, 1303, 1369
Tru1I TTAA 3 cut(s) 464, 725, 798
Tru9I TTAA 3 cut(s) 464, 725, 798
TscAI CASTG 7 cut(s) 649, 879, 1066, 1071, 1425, 1462, 1616
TseFI GTSAC 5 cut(s) 432, 662, 874, 1342, 1408
TseI GCWGC 3 cut(s) 471, 834, 1352
Tsp45I GTSAC 5 cut(s) 432, 662, 874, 1342, 1408
TspDTI ATGAA 7 cut(s) 87, 194, 604, 798, 809, 1232, 1241
TspGWI ACGGA 3 cut(s) 561, 1272, 1371
TspRI CASTG 7 cut(s) 649, 879, 1066, 1071, 1425, 1462, 1616
VpaK11BI GGWCC 1 cut(s) 1520
XapI RAATTY 1 cut(s) 707
XceI RCATGY 2 cut(s) 1047, 1542
XcmI CCANNNNNNNNNTGG 2 cut(s) 790, 1271
XmnI GAANNNNTTC 1 cut(s) 567
ZrmI AGTACT 1 cut(s) 299
Zsp2I ATGCAT 1 cut(s) 1049
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.