pycom14g11990

Germin-like protein subfamily 3 member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016129
Physical Location & Seq
Reverse (-)
15090499 .. 15092905
2407 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1269 bp
ATGGCACTAAATATTGTCTCAGGCCGAACCAATCCTTGTATACTGGATGCTAAAATTGGTGCCAGAACATGGTACCCACAAGCTGCTGAAGATTATCTCCAAAAGTGTCTTAAGAAAGATAGAGAAACTGCGTGCCTGGAACTGGGGTTCAGGATATCTGGACTTCAAGTGTATGGAAACAAACTGACTGGACTTTGGAAGCCCGACAGGCACGTTATTCAGAGCAGTAGCGTTGAAGATTCTAAGTTAGCTCTTAGGAAATTTATATCTTCTAACCCATCTACCGATTCAGATATCAAACCAGATTGTTCTTTCGCATCGACTGTCTATGGTGGTCCTGATGGGATATTGGCACAGCTGTTGGTACTGAAGTCATGGTTTGAGGAGCAAACGTTTTACCATTTCTATTCTTGTTCAGTTCTGATGGTTTATGACAAGGAATCGATACTGCAGGGGAAGAATCGAGGTGCTCAAATTAAACTTGTTGATTTTGCGCATGTCATTGACGGGAGAGGTGTAATCGACCATAATTTTTTGGGCGGGCTCTTCTTTGGCCGGACATTGATGTGTTCCGGCCTTCCGGACCGAAGAACTTCTCCAAATGTTCACACACAAGTCATGCTACAAATCATTGTTGGGGTCCCATCAAATGGAAACAGGGCTGACTCCGATAACCTCCAGGACACTTGCCCTACGTCCCCGCCAGGAAAGCAAACCATCTTCATCAATGGCTTCCCGTGCAAGAATCCAAACAGCATCGCTGCCCCCGACTTCAAAACCTCCAAGCTAACCGAACCTGGCAACACAGACAATTTCCTTGGCTCCGCAGTGACTCTAGTCACTGCTGCAGACTTTCCAGGCCTCAACACGCTTGGCCTCTCAATTGCGCGAACAGACTTGAGAGTAGATGGCATGGTGAGCCTCCACTCCCACCCTCGGGCATCCGAACTGTTCTTTGTGAGCAAAGGAATTGTGCTTGCCGGGTTTATTGACACCAAAAACCAGCCATTCCAACAGGTTCTTGCAGAAGGAGATGTGTTTGTTTTCCCAAAGGGTTTGCTTCATTTTTCCCTGAATTTTGGGTATGATTTCGCCACTGTTTTCTCAGTTTTCAACAGCCAGAATCCAGGGGTTGTGGAAATTTCTGGTGCCATATTTCAATCTGATCTGGATATGATTGACAGGATGACAGAGGGGTTTCTCACTCATCACATCGGAAATCTCACTCTTAACAAGCGGCAATTCAGAAAGTTAAATCCTATCATTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005618 GO:0005623 GO:0030312 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

423

Amino Acids

46.44

Weight (kDa)

7.13

Isoelectric Point (pI)

31.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
IPK PF03770 4 - 182 8.2e-39 Inositol polyphosphate kinase
Cupin_1 PF00190 262 - 386 7.3e-34 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016129)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61750
fragaria_vesca FvH4_5g09990
malus_domestica MD14G1144300.v1.1
prunus_persica Prupe.5G138500_v2.0.a1
pyrus_communis pycom14g11990
rosa_chinensis RchiOBHm_Chr7g0188271
rosa_laevigata RLG00000004712
rosa_multiflora Rmu_sc0008750.1_g000009
rosa_roxburghii Rroxscaffold_3G00266540
rosa_rugosa Rorug06G0489600
rosa_samantha Rh7AG094000 Rh7BG095400 Rh7CG095800 Rh7DG096000
rosa_wichuraiana Rw7G008100

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 495
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 3 cut(s) 59, 72, 1148
AccB7I CCANNNNNTGG 2 cut(s) 69, 650
AccI GTMKAC 1 cut(s) 40
AccII CGCG 1 cut(s) 889
AccIII TCCGGA 1 cut(s) 580
AciI CCGC 4 cut(s) 540, 701, 825, 1237
AclI AACGTT 1 cut(s) 392
AcoI YGGCCR 1 cut(s) 553
AcsI RAATTY 3 cut(s) 260, 1075, 1140
AcuI CTGAAG 2 cut(s) 108, 389
AfaI GTAC 2 cut(s) 74, 366
AfiI CCNNNNNNNGG 5 cut(s) 69, 142, 650, 936, 937
AflII CTTAAG 1 cut(s) 110
AgsI TTSAA 5 cut(s) 167, 236, 775, 1114, 1160
AjnI CCWGG 6 cut(s) 135, 678, 703, 796, 856, 1126
AloI GAACNNNNNNTCC 2 cut(s) 131, 163
AluBI AGCT 4 cut(s) 83, 251, 358, 787
AluI AGCT 4 cut(s) 83, 251, 358, 787
Alw21I GWGCWC 1 cut(s) 472
Alw26I GTCTC 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 936
Aor13HI TCCGGA 1 cut(s) 580
AoxI GGCC 5 cut(s) 22, 553, 574, 859, 874
ApeKI GCWGC 3 cut(s) 83, 761, 845
ApoI RAATTY 3 cut(s) 260, 1075, 1140
Asp700I GAANNNNTTC 1 cut(s) 592
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 2 cut(s) 496, 889
AspS9I GGNCC 3 cut(s) 335, 583, 640
AsuC2I CCSGG 1 cut(s) 982
AsuHPI GGTGA 1 cut(s) 928
AvaI CYCGRG 1 cut(s) 936
AvaII GGWCC 3 cut(s) 335, 583, 640
BaeI ACNNNNGTAYC 2 cut(s) 356, 389
BanI GGYRCC 3 cut(s) 59, 72, 1148
BanII GRGCYC 1 cut(s) 546
Bbv12I GWGCWC 1 cut(s) 472
BbvI GCAGC 3 cut(s) 70, 748, 832
BccI CCATC 6 cut(s) 286, 335, 418, 652, 725, 902
BciT130I CCWGG 6 cut(s) 137, 680, 705, 798, 858, 1128
BcnI CCSGG 1 cut(s) 982
BcoDI GTCTC 1 cut(s) 22
BfaI CTAG 1 cut(s) 836
BfmI CTRYAG 2 cut(s) 449, 846
BfrI CTTAAG 1 cut(s) 110
BglI GCCNNNNNGGC 1 cut(s) 208
BisI GCNGC 4 cut(s) 84, 762, 846, 1238
BlsI GCNGC 4 cut(s) 85, 763, 847, 1239
Bme1390I CCNGG 7 cut(s) 137, 680, 705, 798, 858, 982, 1128
Bme18I GGWCC 3 cut(s) 335, 583, 640
BmeT110I CYCGRG 1 cut(s) 936
BmgT120I GGNCC 3 cut(s) 335, 583, 640
BmiI GGNNCC 6 cut(s) 61, 74, 641, 642, 823, 1150
BmrFI CCNGG 7 cut(s) 137, 680, 705, 798, 858, 982, 1128
BmrI ACTGGG 1 cut(s) 152
BmsI GCATC 4 cut(s) 37, 326, 765, 950
BmuI ACTGGG 1 cut(s) 152
BoxI GACNNNNGTC 1 cut(s) 836
BpmI CTGGAG 1 cut(s) 662
BpuEI CTTGAG 1 cut(s) 919
BpuMI CCSGG 1 cut(s) 982
Bsa29I ATCGAT 1 cut(s) 443
BsaJI CCNNGG 3 cut(s) 817, 935, 1127
BsaWI WCCGGW 1 cut(s) 580
BsaXI ACNNNNNCTCC 2 cut(s) 377, 407
Bsc4I CCNNNNNNNGG 5 cut(s) 69, 142, 650, 936, 937
Bse1I ACTGG 3 cut(s) 48, 147, 193
BseAI TCCGGA 1 cut(s) 580
BseBI CCWGG 6 cut(s) 137, 680, 705, 798, 858, 1128
BseCI ATCGAT 1 cut(s) 443
BseDI CCNNGG 3 cut(s) 817, 935, 1127
BseGI GGATG 3 cut(s) 52, 941, 1191
BseLI CCNNNNNNNGG 5 cut(s) 69, 142, 650, 936, 937
BseMII CTCAG 2 cut(s) 33, 1119
BseNI ACTGG 3 cut(s) 48, 147, 193
BseRI GAGGAG 1 cut(s) 398
BseXI GCAGC 3 cut(s) 70, 748, 832
Bsh1236I CGCG 1 cut(s) 889
BshFI GGCC 5 cut(s) 24, 555, 576, 861, 876
BshNI GGYRCC 3 cut(s) 59, 72, 1148
BshVI ATCGAT 1 cut(s) 443
BsiHKAI GWGCWC 1 cut(s) 472
BsiHKCI CYCGRG 1 cut(s) 936
BsiSI CCGG 4 cut(s) 556, 573, 581, 981
BslFI GGGAC 2 cut(s) 626, 682
BslI CCNNNNNNNGG 5 cut(s) 69, 142, 650, 936, 937
BsmAI GTCTC 1 cut(s) 22
BsmFI GGGAC 2 cut(s) 626, 682
BsnI GGCC 5 cut(s) 24, 555, 576, 861, 876
BsoBI CYCGRG 1 cut(s) 936
Bsp1286I GDGCHC 2 cut(s) 472, 546
Bsp13I TCCGGA 1 cut(s) 580
Bsp143I GATC 1 cut(s) 1165
BspACI CCGC 4 cut(s) 540, 701, 825, 1237
BspANI GGCC 5 cut(s) 24, 555, 576, 861, 876
BspCNI CTCAG 2 cut(s) 32, 1118
BspDI ATCGAT 1 cut(s) 443
BspEI TCCGGA 1 cut(s) 580
BspFNI CGCG 1 cut(s) 889
BspLI GGNNCC 6 cut(s) 61, 74, 641, 642, 823, 1150
BspMAI CTGCAG 2 cut(s) 453, 850
BspQI GCTCTTC 1 cut(s) 551
BspT107I GGYRCC 3 cut(s) 59, 72, 1148
BspTI CTTAAG 1 cut(s) 110
BsrI ACTGG 3 cut(s) 48, 147, 193
BssECI CCNNGG 3 cut(s) 817, 935, 1127
BssMI GATC 1 cut(s) 1165
BssNAI GTATAC 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 817
Bst1107I GTATAC 1 cut(s) 41
Bst2UI CCWGG 6 cut(s) 137, 680, 705, 798, 858, 1128
Bst4CI ACNGT 3 cut(s) 325, 951, 1099
Bst6I CTCTTC 1 cut(s) 551
BstAFI CTTAAG 1 cut(s) 110
BstC8I GCNNGC 3 cut(s) 133, 542, 978
BstDEI CTNAG 4 cut(s) 19, 243, 254, 1105
BstF5I GGATG 3 cut(s) 52, 941, 1191
BstFNI CGCG 1 cut(s) 889
BstHHI GCGC 2 cut(s) 496, 889
BstKTI GATC 1 cut(s) 1168
BstMAI GTCTC 1 cut(s) 22
BstMBI GATC 1 cut(s) 1165
BstMWI GCNNNNNNNGC 4 cut(s) 208, 709, 738, 918
BstNI CCWGG 6 cut(s) 137, 680, 705, 798, 858, 1128
BstNSI RCATGY 1 cut(s) 500
BstPAI GACNNNNGTC 1 cut(s) 836
BstSCI CCNGG 7 cut(s) 135, 678, 703, 796, 856, 980, 1126
BstSFI CTRYAG 2 cut(s) 449, 846
BstUI CGCG 1 cut(s) 889
BstV1I GCAGC 3 cut(s) 70, 748, 832
BstZ17I GTATAC 1 cut(s) 41
Bsu15I ATCGAT 1 cut(s) 443
BsuRI GGCC 5 cut(s) 24, 555, 576, 861, 876
BsuTUI ATCGAT 1 cut(s) 443
BtgZI GCGATG 1 cut(s) 742
BtsCI GGATG 3 cut(s) 52, 941, 1191
BtsI GCAGTG 2 cut(s) 834, 840
BtsIMutI CAGTG 3 cut(s) 834, 840, 1095
Cac8I GCNNGC 3 cut(s) 133, 542, 978
CfoI GCGC 2 cut(s) 496, 889
Cfr13I GGNCC 3 cut(s) 335, 583, 640
ClaI ATCGAT 1 cut(s) 443
CpoI CGGWCCG 1 cut(s) 583
Csp6I GTAC 2 cut(s) 73, 365
CspI CGGWCCG 1 cut(s) 583
CviAII CATG 5 cut(s) 69, 375, 497, 619, 913
CviQI GTAC 2 cut(s) 73, 365
DdeI CTNAG 4 cut(s) 19, 243, 254, 1105
DpnI GATC 1 cut(s) 1167
DpnII GATC 1 cut(s) 1165
EaeI YGGCCR 1 cut(s) 553
Eam1104I CTCTTC 1 cut(s) 551
EarI CTCTTC 1 cut(s) 551
Eco130I CCWWGG 1 cut(s) 817
Eco147I AGGCCT 1 cut(s) 861
Eco24I GRGCYC 1 cut(s) 546
Eco32I GATATC 2 cut(s) 156, 295
Eco47I GGWCC 3 cut(s) 335, 583, 640
Eco57I CTGAAG 2 cut(s) 108, 389
Eco88I CYCGRG 1 cut(s) 936
EcoO109I RGGNCCY 1 cut(s) 640
EcoRII CCWGG 6 cut(s) 135, 678, 703, 796, 856, 1126
EcoRV GATATC 2 cut(s) 156, 295
EcoT14I CCWWGG 1 cut(s) 817
EcoT38I GRGCYC 1 cut(s) 546
ErhI CCWWGG 1 cut(s) 817
FaeI CATG 5 cut(s) 72, 378, 500, 622, 916
FaqI GGGAC 2 cut(s) 626, 682
FatI CATG 5 cut(s) 68, 374, 496, 618, 912
FauI CCCGC 2 cut(s) 533, 708
FblI GTMKAC 1 cut(s) 40
Fnu4HI GCNGC 4 cut(s) 84, 762, 846, 1238
FokI GGATG 3 cut(s) 59, 928, 1198
FriOI GRGCYC 1 cut(s) 546
Fsp4HI GCNGC 4 cut(s) 84, 762, 846, 1238
FspBI CTAG 1 cut(s) 836
FspI TGCGCA 1 cut(s) 495
GlaI GCGC 2 cut(s) 495, 888
GluI GCNGC 4 cut(s) 84, 762, 846, 1238
GsuI CTGGAG 1 cut(s) 662
HaeIII GGCC 5 cut(s) 24, 555, 576, 861, 876
HapII CCGG 4 cut(s) 556, 573, 581, 981
HhaI GCGC 2 cut(s) 496, 889
Hin1II CATG 5 cut(s) 72, 378, 500, 622, 916
Hin6I GCGC 2 cut(s) 494, 887
HinP1I GCGC 2 cut(s) 494, 887
HinfI GANTC 8 cut(s) 239, 287, 440, 460, 665, 745, 832, 1123
HpaII CCGG 4 cut(s) 556, 573, 581, 981
HphI GGTGA 1 cut(s) 928
Hpy166II GTNNAC 2 cut(s) 41, 607
Hpy188I TCNGA 8 cut(s) 222, 292, 423, 670, 946, 1165, 1217, 1247
Hpy188III TCNNGA 5 cut(s) 151, 159, 338, 581, 1169
Hpy8I GTNNAC 2 cut(s) 41, 607
HpyAV CCTTC 2 cut(s) 587, 1022
HpyCH4III ACNGT 3 cut(s) 325, 951, 1099
HpyCH4IV ACGT 3 cut(s) 213, 392, 695
HpyCH4V TGCA 4 cut(s) 451, 741, 848, 1025
HpyF10VI GCNNNNNNNGC 4 cut(s) 208, 709, 738, 918
HpyF3I CTNAG 4 cut(s) 19, 243, 254, 1105
HpySE526I ACGT 3 cut(s) 213, 392, 695
Hsp92II CATG 5 cut(s) 72, 378, 500, 622, 916
HspAI GCGC 2 cut(s) 494, 887
KflI GGGWCCC 1 cut(s) 640
Kpn2I TCCGGA 1 cut(s) 580
KpnI GGTACC 1 cut(s) 76
Kzo9I GATC 1 cut(s) 1165
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 2 cut(s) 385, 827
Lsp1109I GCAGC 3 cut(s) 70, 748, 832
LweI GCATC 4 cut(s) 37, 326, 765, 950
MaeI CTAG 1 cut(s) 836
MaeII ACGT 3 cut(s) 213, 392, 695
MaeIII GTNAC 2 cut(s) 829, 838
MalI GATC 1 cut(s) 1167
MboI GATC 1 cut(s) 1165
MboII GAAGA 7 cut(s) 101, 248, 261, 469, 538, 600, 712
MfeI CAATTG 1 cut(s) 882
MhlI GDGCHC 2 cut(s) 472, 546
MlyI GAGTC 2 cut(s) 659, 826
MmeI TCCRAC 1 cut(s) 1036
MroI TCCGGA 1 cut(s) 580
MroXI GAANNNNTTC 1 cut(s) 592
MseI TTAA 4 cut(s) 111, 477, 1230, 1253
MslI CAYNNNNRTG 1 cut(s) 565
MspA1I CMGCKG 1 cut(s) 358
MspCI CTTAAG 1 cut(s) 110
MspI CCGG 4 cut(s) 556, 573, 581, 981
MspR9I CCNGG 7 cut(s) 137, 680, 705, 798, 858, 982, 1128
MunI CAATTG 1 cut(s) 882
MvaI CCWGG 6 cut(s) 137, 680, 705, 798, 858, 1128
MvnI CGCG 1 cut(s) 889
MwoI GCNNNNNNNGC 4 cut(s) 208, 709, 738, 918
NciI CCSGG 1 cut(s) 982
NdeII GATC 1 cut(s) 1165
NlaIII CATG 5 cut(s) 72, 378, 500, 622, 916
NlaIV GGNNCC 6 cut(s) 61, 74, 641, 642, 823, 1150
NmuCI GTSAC 2 cut(s) 829, 838
NsbI TGCGCA 1 cut(s) 495
NspI RCATGY 1 cut(s) 500
PceI AGGCCT 1 cut(s) 861
PciSI GCTCTTC 1 cut(s) 551
PcsI WCGNNNNNNNCGW 1 cut(s) 765
PdmI GAANNNNTTC 1 cut(s) 592
PfeI GAWTC 6 cut(s) 239, 287, 440, 460, 745, 1123
PflMI CCANNNNNTGG 2 cut(s) 69, 650
PfoI TCCNGGA 1 cut(s) 678
PkrI GCNGC 4 cut(s) 85, 763, 847, 1239
PleI GAGTC 2 cut(s) 659, 826
PpsI GAGTC 2 cut(s) 659, 826
PpuMI RGGWCCY 1 cut(s) 640
PshAI GACNNNNGTC 1 cut(s) 836
Psp1406I AACGTT 1 cut(s) 392
Psp5II RGGWCCY 1 cut(s) 640
Psp6I CCWGG 6 cut(s) 135, 678, 703, 796, 856, 1126
PspGI CCWGG 6 cut(s) 135, 678, 703, 796, 856, 1126
PspN4I GGNNCC 6 cut(s) 61, 74, 641, 642, 823, 1150
PspPI GGNCC 3 cut(s) 335, 583, 640
PspPPI RGGWCCY 1 cut(s) 640
PstI CTGCAG 2 cut(s) 453, 850
PvuII CAGCTG 1 cut(s) 358
RsaI GTAC 2 cut(s) 74, 366
RsaNI GTAC 2 cut(s) 73, 365
RseI CAYNNNNRTG 1 cut(s) 565
Rsr2I CGGWCCG 1 cut(s) 583
RsrII CGGWCCG 1 cut(s) 583
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 4 cut(s) 111, 477, 1230, 1253
SatI GCNGC 4 cut(s) 84, 762, 846, 1238
Sau3AI GATC 1 cut(s) 1165
Sau96I GGNCC 3 cut(s) 335, 583, 640
SchI GAGTC 2 cut(s) 659, 826
ScrFI CCNGG 7 cut(s) 137, 680, 705, 798, 858, 982, 1128
SduI GDGCHC 2 cut(s) 472, 546
SfaNI GCATC 4 cut(s) 37, 326, 765, 950
SfcI CTRYAG 2 cut(s) 449, 846
SinI GGWCC 3 cut(s) 335, 583, 640
SmiMI CAYNNNNRTG 1 cut(s) 565
SmlI CTYRAG 2 cut(s) 110, 898
SmoI CTYRAG 2 cut(s) 110, 898
SseBI AGGCCT 1 cut(s) 861
SsiI CCGC 4 cut(s) 540, 701, 825, 1237
SspI AATATT 1 cut(s) 13
SspMI CTAG 1 cut(s) 836
StuI AGGCCT 1 cut(s) 861
StyD4I CCNGG 7 cut(s) 135, 678, 703, 796, 856, 980, 1126
StyI CCWWGG 1 cut(s) 817
TaaI ACNGT 3 cut(s) 325, 951, 1099
TaiI ACGT 3 cut(s) 216, 395, 698
TaqI TCGA 4 cut(s) 320, 443, 463, 522
TaqII GACCGA 1 cut(s) 600
TauI GCSGC 1 cut(s) 1240
TfiI GAWTC 6 cut(s) 239, 287, 440, 460, 745, 1123
Tru1I TTAA 4 cut(s) 111, 477, 1230, 1253
Tru9I TTAA 4 cut(s) 111, 477, 1230, 1253
TscAI CASTG 3 cut(s) 834, 847, 1102
TseFI GTSAC 2 cut(s) 829, 838
TseI GCWGC 3 cut(s) 83, 761, 845
Tsp45I GTSAC 2 cut(s) 829, 838
TspDTI ATGAA 2 cut(s) 712, 1052
TspRI CASTG 3 cut(s) 834, 847, 1102
Van91I CCANNNNNTGG 2 cut(s) 69, 650
Vha464I CTTAAG 1 cut(s) 110
VpaK11BI GGWCC 3 cut(s) 335, 583, 640
XapI RAATTY 3 cut(s) 260, 1075, 1140
XceI RCATGY 1 cut(s) 500
XmiI GTMKAC 1 cut(s) 40
XmnI GAANNNNTTC 1 cut(s) 592
XspI CTAG 1 cut(s) 836
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.