pycom14g12290

ADP-ribosylation factor GTPase-activating protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0011770
Physical Location & Seq
Forward (+)
15309889 .. 15314904
5016 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2418 bp
ATGCATTTCGCAAAGCTCGATGATTCGCCTATGTTCCGCCAACAGATACAAGGGTTGGAGGAAAGTGCAGAGTCACTCAGGGGAAGATGTTATAAGTTTTACAAAGGATGTCGAAAGCATACAGAAGGGCTTGGAGAAGCGTATGATGGTGAAATCGCTTTTATAACTGCCCTCGAAACTTTTGGGGGAGGGCATAATGATCCTATTTTTTCAGATTTAGGAGGCCATGTTATGACCAGATTCACAACTGCATTGAGAGAAATCGCGACGTACAAGGAAGTTTTTCGTTCACAGTTAGAGCACATGCTAAATGATAAGCTGATGCACATTGTCCATGTTGACCTCCATGATGTCAAGGAAGCCCGAAAGCGTTTTGACAAGACTTCACTTGTATATGATCAGGCACGTGAGAAGTTTCTGTCGTTGAGGAAGAGTACGAGGATGGATATAGCTGCTAGTATAGAGGAGGAACTGCAGAATGCAAGATCCTCGTTTGAGCAAGCCCGCTTCAACCTGGTTACCGTTCTATCTGATATCGAGGCCAAAAAGAGGTTTGAGTTTTCGGAAGCTGTTAGTGGCATGATGGATGCTCATCTCCGTTACTTCAAACACAGTTATGAGTTACTGCATGCAATGGAACCGTATATCCATCAGGTTTTGGCTTATGCACAACAGACAAGAGAAAGTTGCAAACAGGAACAGATATCTCTTAATGAAAGGATACTAGAGTACAAAAGGCAGATTGATCGGGAAAGTACACAGTCCTTGAGCAGTATCCATGGTTCCCCCAGTGGAGATGGTCCACAGCCTCTCTCTCGGAAATCACATAAAGTGATCGAGGAAGTGATGCAATCTGCTGCAAAGGGGAAGGTACAAACCATTCGGCAAGGATATCTATCAAAACGTTCCTCCAACTTGAGAGGTGACTGGAAGCGAAGGTATTTCATCCTTGATAGTCGAGGGATGCTGTACTACTATCGCAAACCATTGACTCGGCCATCTTATGGGAGTCAATCTTCTCCACAGAAGAATAATTACTCTGAAAATGGCTCCGGGATTTTGAGTCGATGGCTTTCATCTCACTACCATGGCGGTGTACATGATGAAAAATCCGTTGCACGCCACACTGTGAACTTGCTGACATCAACGATAAAGGTTGATGCAGACCAGACAGACTTAAGGTTCTGCTTCAGAATTATATCACCAATAAAAATCTACACTTTGCAGGCAGAGAATGCACTCGATCAAATGGATTGGATTGAGAAAATCACTGGAGTAATTGCTTCACTATTGAGTTTCCAGACATCTGAGAGGCGTCTCTCGACTAGCCCTACAGGAAGCGACGATCAATTCTCTGGAAACGAGGGTAGTTCATTAGAAAGCGCTCCTGATGATGAGCAGACAGCTATTCCGGAGCTCTCACCTAAGATCACAGCTTGTAATTATCTCCGAATGTCTAAAAGTTTGCAGCCGCACAAGTTCAAACCGAAAAGTGAGAAACTGATTGACTTACTGAGAAGAGTGTCTGGTAATGAAAAATGTGCAGATTGTGGTGCACCTGAACCAGATTGGGCATCTTTGAACCTTGGGATTCTTATTTGCATCGAATGTTCTGGTGTCCATCGTAATCTTGGTGTACATATATCAAAGGTTCGGTCACTGACACTTGATGTGAAAGTATGGGAACCTTCTGTCTTAACCTTGTTTCAATCACTGGGAAATACTTTCGTAAACTCGATTTGGGAGGAGATGTTGTATTCAAGTTCTTTGCAGGCTGATAACATGCCTATAGGTTTCTCCAGTGCTGATAGAAATGAGCCGTTTCTTATGATAAAGCCATGTTATGACGATCTTATTTCGACAAAAGAGAGGTATATTCATGCAAAGTATGCAGAAAAACTTTTTGTTCGTCGTACAAAAAACAATCAGCAGCTCCACGTAACGGGGCAACAGTTGTGCAAAAGTGTCCGAGCGAATGACAAGAAAGCAGTTTACCGCTATATTATTGGTTATGAAGCAGATGTTAATGCTAGATCAGTGCAAGCATTAGATGATCATTCCGAAGACAAGCCTTCGTCGAGTAACTTAAATTCTGTCACTAGAAGTGAAATTCAGCCCGCAAAAGACAGCGCTGAGGACTGTTCCCTGCTTCACCTAGCTTGCCAAAGTGCCGATATTGGCATGGTAGAGCTACTTTTACAGTATGGTGCGCACGTAAACGCTACTGATTTAAAAGGTCAAACACCGCTGCATTATTGCGTTATCAGAGGGAACATTGCTATTGCAAAGTTGCTTCTTACGAGGGGAGCTGATCCTCGAGCCACTGACAGAGAAGGTAAAACACCAAGTGAGCTTGTATCAGAATCCGCCTCGAAAGACAATGGGATGCTGGCTCTTTTAACAAACTCCGGCAGATGA

Protein Analysis

806

Amino Acids

90.92

Weight (kDa)

7.85

Isoelectric Point (pI)

52.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BAR_3 PF16746 6 - 234 3.6e-35 BAR domain of APPL family
BAR PF03114 69 - 216 5.6e-14 BAR domain
BAR_2 PF10455 77 - 210 5.2e-06 Bin/amphiphysin/Rvs domain for vesicular trafficking
PH PF00169 294 - 427 3.2e-11 PH domain
ArfGap PF01412 501 - 591 2.6e-31 Putative GTPase activating protein for Arf
Ank_2 PF12796 712 - 776 2.1e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 717 - 766 4.7e-11 Ankyrin repeats (many copies)
Ank_5 PF13857 732 - 784 1.4e-08 Ankyrin repeats (many copies)
Ank PF00023 746 - 777 1.7e-07 Ankyrin repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes (Group: OG0011770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G61980
fragaria_vesca FvH4_5g09370 FvH4_5g09370 FvH4_5g09370
malus_domestica MD06G1131800.v1.1 MD14G1148100.v1.1
prunus_persica Prupe.5G142800_v2.0.a1
pyrus_communis pycom06g12340 pycom14g12290
rosa_chinensis RchiOBHm_Chr7g0188801
rosa_laevigata RLG00000004658
rosa_multiflora Rmu_sc0003642.1_g000001
rosa_roxburghii Rroxscaffold_3G00266010
rosa_rugosa Rorug06G0494100
rosa_samantha Rh7AG098800 Rh7BG100500 Rh7CG102000 Rh7DG100600
rosa_wichuraiana Rw7G008510

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 93, 164
Acc16I TGCGCA 1 cut(s) 2211
AccB7I CCANNNNNTGG 1 cut(s) 1004
AccII CGCG 1 cut(s) 266
AccIII TCCGGA 1 cut(s) 1411
AciI CCGC 8 cut(s) 37, 505, 1092, 1472, 1996, 2118, 2246, 2367
AclI AACGTT 1 cut(s) 904
AclWI GGATC 3 cut(s) 194, 480, 2306
AcoI YGGCCR 1 cut(s) 995
AcsI RAATTY 2 cut(s) 2089, 2109
AcuI CTGAAG 1 cut(s) 1174
AcvI CACGTG 1 cut(s) 407
AcyI GRCGYC 1 cut(s) 1315
AdeI CACNNNGTG 2 cut(s) 1129, 2348
AfaI GTAC 9 cut(s) 272, 436, 731, 757, 873, 971, 1098, 1638, 1915
AfeI AGCGCT 2 cut(s) 1384, 2131
AfiI CCNNNNNNNGG 3 cut(s) 549, 1004, 1942
AflII CTTAAG 1 cut(s) 1177
AgsI TTSAA 6 cut(s) 511, 607, 1483, 1582, 1709, 1761
AjnI CCWGG 1 cut(s) 513
AloI GAACNNNNNNTCC 2 cut(s) 630, 662
Alw21I GWGCWC 3 cut(s) 303, 1419, 1558
Alw26I GTCTC 1 cut(s) 1322
Alw44I GTGCAC 1 cut(s) 1554
AlwI GGATC 3 cut(s) 194, 480, 2306
Ama87I CYCGRG 1 cut(s) 2316
Aor13HI TCCGGA 1 cut(s) 1411
Aor51HI AGCGCT 2 cut(s) 1384, 2131
AoxI GGCC 3 cut(s) 223, 540, 995
ApaLI GTGCAC 1 cut(s) 1554
ApeKI GCWGC 5 cut(s) 452, 857, 1468, 1930, 2248
ApoI RAATTY 2 cut(s) 2089, 2109
ArsI GACNNNNNNTTYG 2 cut(s) 1640, 1672
Asp700I GAANNNNTTC 1 cut(s) 282
AspLEI GCGC 3 cut(s) 1385, 2132, 2212
AspS9I GGNCC 1 cut(s) 800
AsuC2I CCSGG 1 cut(s) 1054
AsuHPI GGTGA 5 cut(s) 161, 935, 1194, 1413, 2144
AvaI CYCGRG 1 cut(s) 2316
AvaII GGWCC 1 cut(s) 800
BaeGI GKGCMC 1 cut(s) 1558
BaeI ACNNNNGTAYC 4 cut(s) 38, 71, 713, 746
BanII GRGCYC 1 cut(s) 1419
BarI GAAGNNNNNNTAC 2 cut(s) 1019, 1051
BbrPI CACGTG 1 cut(s) 407
BbsI GAAGAC 1 cut(s) 2070
Bbv12I GWGCWC 3 cut(s) 303, 1419, 1558
BbvCI CCTCAGC 1 cut(s) 2133
BbvI GCAGC 5 cut(s) 439, 844, 1480, 1942, 2235
BccI CCATC 8 cut(s) 140, 436, 577, 657, 791, 1006, 1062, 1629
BceAI ACGGC 1 cut(s) 1804
BcgI CGANNNNNNTGC 4 cut(s) 471, 505, 728, 762
BciT130I CCWGG 1 cut(s) 515
BciVI GTATCC 2 cut(s) 714, 785
BclI TGATCA 2 cut(s) 397, 2053
BcnI CCSGG 1 cut(s) 1054
BcoDI GTCTC 1 cut(s) 1322
BfaI CTAG 6 cut(s) 456, 725, 1326, 2031, 2100, 2156
BfmI CTRYAG 3 cut(s) 473, 1332, 1788
BfoI RGCGCY 2 cut(s) 1386, 2133
BfrI CTTAAG 1 cut(s) 1177
BfuI GTATCC 2 cut(s) 714, 785
BisI GCNGC 6 cut(s) 453, 858, 1469, 1472, 1931, 2249
BlsI GCNGC 6 cut(s) 454, 859, 1470, 1473, 1932, 2250
Bme1390I CCNGG 2 cut(s) 515, 1054
Bme18I GGWCC 1 cut(s) 800
BmeT110I CYCGRG 1 cut(s) 2316
BmgT120I GGNCC 1 cut(s) 800
BmiI GGNNCC 4 cut(s) 639, 784, 1051, 1686
BmrFI CCNGG 2 cut(s) 515, 1054
BmrI ACTGGG 2 cut(s) 783, 1724
BmsI GCATC 8 cut(s) 312, 577, 837, 954, 1150, 1583, 1611, 2376
BmuI ACTGGG 2 cut(s) 783, 1724
BpiI GAAGAC 1 cut(s) 2070
BpmI CTGGAG 2 cut(s) 1293, 1783
Bpu10I CCTNAGC 1 cut(s) 2133
BpuEI CTTGAG 2 cut(s) 787, 937
BpuMI CCSGG 1 cut(s) 1054
BsaAI YACGTR 3 cut(s) 407, 1939, 2215
BsaBI GATNNNNATC 2 cut(s) 591, 895
BsaHI GRCGYC 1 cut(s) 1315
BsaJI CCNNGG 3 cut(s) 778, 1087, 1585
BsaWI WCCGGW 1 cut(s) 1411
BsaXI ACNNNNNCTCC 6 cut(s) 213, 243, 1736, 1739, 1766, 1769
Bsc4I CCNNNNNNNGG 3 cut(s) 549, 1004, 1942
Bse1I ACTGG 5 cut(s) 789, 932, 1276, 1719, 1800
Bse3DI GCAATG 2 cut(s) 639, 2274
Bse8I GATNNNNATC 2 cut(s) 591, 895
BseAI TCCGGA 1 cut(s) 1411
BseBI CCWGG 1 cut(s) 515
BseDI CCNNGG 3 cut(s) 778, 1087, 1585
BseGI GGATG 6 cut(s) 113, 447, 592, 945, 969, 2391
BseJI GATNNNNATC 2 cut(s) 591, 895
BseLI CCNNNNNNNGG 3 cut(s) 549, 1004, 1942
BseMI GCAATG 2 cut(s) 639, 2274
BseMII CTCAG 4 cut(s) 91, 1299, 1505, 2124
BseNI ACTGG 5 cut(s) 789, 932, 1276, 1719, 1800
BseRI GAGGAG 2 cut(s) 479, 1760
BseSI GKGCMC 1 cut(s) 1558
BseXI GCAGC 5 cut(s) 439, 844, 1480, 1942, 2235
BsgI GTGCAG 2 cut(s) 87, 1563
Bsh1236I CGCG 1 cut(s) 266
BshFI GGCC 3 cut(s) 225, 542, 997
BsiHKAI GWGCWC 3 cut(s) 303, 1419, 1558
BsiHKCI CYCGRG 1 cut(s) 2316
BsiSI CCGG 3 cut(s) 1053, 1412, 2409
BslI CCNNNNNNNGG 3 cut(s) 549, 1004, 1942
BsmAI GTCTC 1 cut(s) 1322
BsmBI CGTCTC 1 cut(s) 1322
BsmI GAATGC 2 cut(s) 484, 1240
BsnI GGCC 3 cut(s) 225, 542, 997
BsoBI CYCGRG 1 cut(s) 2316
Bsp1286I GDGCHC 3 cut(s) 303, 1419, 1558
Bsp13I TCCGGA 1 cut(s) 1411
Bsp1407I TGTACA 2 cut(s) 1096, 1636
Bsp19I CCATGG 2 cut(s) 778, 1087
Bsp68I TCGCGA 1 cut(s) 266
BspACI CCGC 8 cut(s) 37, 505, 1092, 1472, 1996, 2118, 2246, 2367
BspANI GGCC 3 cut(s) 225, 542, 997
BspCNI CTCAG 4 cut(s) 90, 1300, 1506, 2125
BspEI TCCGGA 1 cut(s) 1411
BspFNI CGCG 1 cut(s) 266
BspLI GGNNCC 4 cut(s) 639, 784, 1051, 1686
BspMAI CTGCAG 1 cut(s) 477
BspPI GGATC 3 cut(s) 194, 480, 2306
BspTI CTTAAG 1 cut(s) 1177
BsrDI GCAATG 2 cut(s) 639, 2274
BsrGI TGTACA 2 cut(s) 1096, 1636
BsrI ACTGG 5 cut(s) 789, 932, 1276, 1719, 1800
BssECI CCNNGG 3 cut(s) 778, 1087, 1585
BssNI GRCGYC 1 cut(s) 1315
BssT1I CCWWGG 3 cut(s) 778, 1087, 1585
Bst2UI CCWGG 1 cut(s) 515
Bst4CI ACNGT 9 cut(s) 294, 523, 614, 642, 762, 1129, 1953, 2141, 2202
Bst6I CTCTTC 2 cut(s) 425, 1513
BstACI GRCGYC 1 cut(s) 1315
BstAFI CTTAAG 1 cut(s) 1177
BstAPI GCANNNNNTGC 2 cut(s) 1235, 1889
BstAUI TGTACA 2 cut(s) 1096, 1636
BstBAI YACGTR 3 cut(s) 407, 1939, 2215
BstDEI CTNAG 5 cut(s) 77, 1308, 1425, 1514, 2133
BstDSI CCRYGG 2 cut(s) 778, 1087
BstEII GGTNACC 1 cut(s) 517
BstF5I GGATG 6 cut(s) 113, 447, 592, 945, 969, 2391
BstFNI CGCG 1 cut(s) 266
BstH2I RGCGCY 2 cut(s) 1386, 2133
BstHHI GCGC 3 cut(s) 1385, 2132, 2212
BstMAI GTCTC 1 cut(s) 1322
BstMWI GCNNNNNNNGC 2 cut(s) 1235, 1889
BstNI CCWGG 1 cut(s) 515
BstNSI RCATGY 3 cut(s) 307, 632, 1786
BstPI GGTNACC 1 cut(s) 517
BstSCI CCNGG 2 cut(s) 513, 1052
BstSFI CTRYAG 3 cut(s) 473, 1332, 1788
BstSLI GKGCMC 1 cut(s) 1558
BstUI CGCG 1 cut(s) 266
BstV1I GCAGC 5 cut(s) 439, 844, 1480, 1942, 2235
BstV2I GAAGAC 1 cut(s) 2070
BstX2I RGATCY 1 cut(s) 485
BstYI RGATCY 1 cut(s) 485
BsuI GTATCC 2 cut(s) 714, 785
BsuRI GGCC 3 cut(s) 225, 542, 997
BtgI CCRYGG 2 cut(s) 778, 1087
BtsCI GGATG 6 cut(s) 113, 447, 592, 945, 969, 2391
BtsIMutI CAGTG 8 cut(s) 796, 1125, 1269, 1658, 1712, 1807, 2043, 2322
BtuMI TCGCGA 1 cut(s) 266
CfoI GCGC 3 cut(s) 1385, 2132, 2212
Cfr13I GGNCC 1 cut(s) 800
CseI GACGC 1 cut(s) 1304
CsiI ACCWGGT 1 cut(s) 513
Csp6I GTAC 9 cut(s) 271, 435, 730, 756, 872, 970, 1097, 1637, 1914
CviQI GTAC 9 cut(s) 271, 435, 730, 756, 872, 970, 1097, 1637, 1914
DdeI CTNAG 5 cut(s) 77, 1308, 1425, 1514, 2133
DraI TTTAAA 1 cut(s) 2232
DraIII CACNNNGTG 2 cut(s) 1129, 2348
EaeI YGGCCR 1 cut(s) 995
Eam1104I CTCTTC 2 cut(s) 425, 1513
EarI CTCTTC 2 cut(s) 425, 1513
EciI GGCGGA 2 cut(s) 26, 2356
Ecl136II GAGCTC 1 cut(s) 1417
Eco130I CCWWGG 3 cut(s) 778, 1087, 1585
Eco24I GRGCYC 1 cut(s) 1419
Eco32I GATATC 3 cut(s) 535, 705, 893
Eco47I GGWCC 1 cut(s) 800
Eco47III AGCGCT 2 cut(s) 1384, 2131
Eco53kI GAGCTC 1 cut(s) 1417
Eco57I CTGAAG 1 cut(s) 1174
Eco72I CACGTG 1 cut(s) 407
Eco88I CYCGRG 1 cut(s) 2316
Eco91I GGTNACC 1 cut(s) 517
EcoICRI GAGCTC 1 cut(s) 1417
EcoO65I GGTNACC 1 cut(s) 517
EcoRII CCWGG 1 cut(s) 513
EcoRV GATATC 3 cut(s) 535, 705, 893
EcoT14I CCWWGG 3 cut(s) 778, 1087, 1585
EcoT22I ATGCAT 1 cut(s) 6
EcoT38I GRGCYC 1 cut(s) 1419
ErhI CCWWGG 3 cut(s) 778, 1087, 1585
Esp3I CGTCTC 1 cut(s) 1322
FauI CCCGC 2 cut(s) 512, 2125
FbaI TGATCA 2 cut(s) 397, 2053
Fnu4HI GCNGC 6 cut(s) 453, 858, 1469, 1472, 1931, 2249
FokI GGATG 6 cut(s) 120, 454, 599, 932, 976, 2398
FriOI GRGCYC 1 cut(s) 1419
Fsp4HI GCNGC 6 cut(s) 453, 858, 1469, 1472, 1931, 2249
FspAI RTGCGCAY 1 cut(s) 2211
FspBI CTAG 6 cut(s) 456, 725, 1326, 2031, 2100, 2156
FspI TGCGCA 1 cut(s) 2211
GlaI GCGC 3 cut(s) 1384, 2131, 2211
GluI GCNGC 6 cut(s) 453, 858, 1469, 1472, 1931, 2249
GsuI CTGGAG 2 cut(s) 1293, 1783
HaeII RGCGCY 2 cut(s) 1386, 2133
HaeIII GGCC 3 cut(s) 225, 542, 997
HapII CCGG 3 cut(s) 1053, 1412, 2409
HgaI GACGC 1 cut(s) 1304
HhaI GCGC 3 cut(s) 1385, 2132, 2212
Hin1I GRCGYC 1 cut(s) 1315
Hin6I GCGC 3 cut(s) 1383, 2130, 2210
HinP1I GCGC 3 cut(s) 1383, 2130, 2210
HincII GTYRAC 1 cut(s) 340
HindII GTYRAC 1 cut(s) 340
HinfI GANTC 8 cut(s) 23, 71, 240, 991, 1009, 1063, 1591, 2363
HpaII CCGG 3 cut(s) 1053, 1412, 2409
HphI GGTGA 5 cut(s) 161, 935, 1194, 1413, 2144
Hpy188III TCNNGA 7 cut(s) 265, 749, 1300, 1321, 1356, 1388, 1412
Hpy99I CGWCG 4 cut(s) 271, 1346, 1914, 2080
HpyAV CCTTC 6 cut(s) 119, 862, 930, 1698, 2082, 2327
HpyCH4III ACNGT 9 cut(s) 294, 523, 614, 642, 762, 1129, 1953, 2141, 2202
HpyCH4IV ACGT 5 cut(s) 269, 406, 904, 1938, 2214
HpyF10VI GCNNNNNNNGC 2 cut(s) 1235, 1889
HpyF3I CTNAG 5 cut(s) 77, 1308, 1425, 1514, 2133
HpySE526I ACGT 5 cut(s) 269, 406, 904, 1938, 2214
Hsp92I GRCGYC 1 cut(s) 1315
HspAI GCGC 3 cut(s) 1383, 2130, 2210
Kpn2I TCCGGA 1 cut(s) 1411
Ksp22I TGATCA 2 cut(s) 397, 2053
LmnI GCTCC 5 cut(s) 1055, 1390, 1414, 1938, 2306
Lsp1109I GCAGC 5 cut(s) 439, 844, 1480, 1942, 2235
LweI GCATC 8 cut(s) 312, 577, 837, 954, 1150, 1583, 1611, 2376
MabI ACCWGGT 1 cut(s) 513
MaeI CTAG 6 cut(s) 456, 725, 1326, 2031, 2100, 2156
MaeII ACGT 5 cut(s) 269, 406, 904, 1938, 2214
MaeIII GTNAC 9 cut(s) 72, 517, 599, 621, 923, 1656, 1939, 2081, 2095
MboII GAAGA 6 cut(s) 96, 442, 1008, 1039, 1530, 2075
MflI RGATCY 1 cut(s) 485
MhlI GDGCHC 3 cut(s) 303, 1419, 1558
MluCI AATT 7 cut(s) 1033, 1194, 1278, 1349, 1441, 2089, 2109
MlyI GAGTC 4 cut(s) 80, 985, 1018, 1072
MmeI TCCRAC 2 cut(s) 36, 936
Mph1103I ATGCAT 1 cut(s) 6
MroI TCCGGA 1 cut(s) 1411
MroXI GAANNNNTTC 1 cut(s) 282
MseI TTAA 7 cut(s) 711, 1178, 1697, 2025, 2087, 2231, 2399
MslI CAYNNNNRTG 3 cut(s) 615, 1086, 1092
MspA1I CMGCKG 1 cut(s) 2248
MspCI CTTAAG 1 cut(s) 1177
MspI CCGG 3 cut(s) 1053, 1412, 2409
MspR9I CCNGG 2 cut(s) 515, 1054
Mva1269I GAATGC 2 cut(s) 484, 1240
MvaI CCWGG 1 cut(s) 515
MvnI CGCG 1 cut(s) 266
MwoI GCNNNNNNNGC 2 cut(s) 1235, 1889
NciI CCSGG 1 cut(s) 1054
NcoI CCATGG 2 cut(s) 778, 1087
NlaIV GGNNCC 4 cut(s) 639, 784, 1051, 1686
NmeAIII GCCGAG 1 cut(s) 973
NmuCI GTSAC 4 cut(s) 72, 923, 1656, 2095
NruI TCGCGA 1 cut(s) 266
NsbI TGCGCA 1 cut(s) 2211
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 3 cut(s) 307, 632, 1786
PaeI GCATGC 1 cut(s) 632
PaeR7I CTCGAG 1 cut(s) 2316
PcsI WCGNNNNNNNCGW 1 cut(s) 15
PctI GAATGC 2 cut(s) 484, 1240
PdmI GAANNNNTTC 1 cut(s) 282
PfeI GAWTC 4 cut(s) 23, 240, 1591, 2363
PflMI CCANNNNNTGG 1 cut(s) 1004
PfoI TCCNGGA 1 cut(s) 1052
PkrI GCNGC 6 cut(s) 454, 859, 1470, 1473, 1932, 2250
PleI GAGTC 4 cut(s) 79, 985, 1017, 1071
PmaCI CACGTG 1 cut(s) 407
PmlI CACGTG 1 cut(s) 407
PpsI GAGTC 4 cut(s) 79, 985, 1017, 1071
Ppu21I YACGTR 3 cut(s) 407, 1939, 2215
PsiI TTATAA 2 cut(s) 93, 164
Psp124BI GAGCTC 1 cut(s) 1419
Psp1406I AACGTT 1 cut(s) 904
Psp6I CCWGG 1 cut(s) 513
PspCI CACGTG 1 cut(s) 407
PspEI GGTNACC 1 cut(s) 517
PspGI CCWGG 1 cut(s) 513
PspN4I GGNNCC 4 cut(s) 639, 784, 1051, 1686
PspPI GGNCC 1 cut(s) 800
PspXI VCTCGAGB 1 cut(s) 2316
PsrI GAACNNNNNNTAC 2 cut(s) 766, 798
PstI CTGCAG 1 cut(s) 477
PsuI RGATCY 1 cut(s) 485
RruI TCGCGA 1 cut(s) 266
RsaI GTAC 9 cut(s) 272, 436, 731, 757, 873, 971, 1098, 1638, 1915
RsaNI GTAC 9 cut(s) 271, 435, 730, 756, 872, 970, 1097, 1637, 1914
RseI CAYNNNNRTG 3 cut(s) 615, 1086, 1092
SacI GAGCTC 1 cut(s) 1419
SaqAI TTAA 7 cut(s) 711, 1178, 1697, 2025, 2087, 2231, 2399
SatI GCNGC 6 cut(s) 453, 858, 1469, 1472, 1931, 2249
Sau96I GGNCC 1 cut(s) 800
SchI GAGTC 4 cut(s) 80, 985, 1018, 1072
ScrFI CCNGG 2 cut(s) 515, 1054
SduI GDGCHC 3 cut(s) 303, 1419, 1558
SexAI ACCWGGT 1 cut(s) 513
SfaNI GCATC 8 cut(s) 312, 577, 837, 954, 1150, 1583, 1611, 2376
SfcI CTRYAG 3 cut(s) 473, 1332, 1788
Sfr274I CTCGAG 1 cut(s) 2316
SinI GGWCC 1 cut(s) 800
SlaI CTCGAG 1 cut(s) 2316
SmiMI CAYNNNNRTG 3 cut(s) 615, 1086, 1092
SmlI CTYRAG 4 cut(s) 766, 916, 1177, 2316
SmoI CTYRAG 4 cut(s) 766, 916, 1177, 2316
SphI GCATGC 1 cut(s) 632
Sse9I AATT 7 cut(s) 1033, 1194, 1278, 1349, 1441, 2089, 2109
SsiI CCGC 8 cut(s) 37, 505, 1092, 1472, 1996, 2118, 2246, 2367
SspMI CTAG 6 cut(s) 456, 725, 1326, 2031, 2100, 2156
SstI GAGCTC 1 cut(s) 1419
StyD4I CCNGG 2 cut(s) 513, 1052
StyI CCWWGG 3 cut(s) 778, 1087, 1585
TaaI ACNGT 9 cut(s) 294, 523, 614, 642, 762, 1129, 1953, 2141, 2202
TaiI ACGT 5 cut(s) 272, 409, 907, 1941, 2217
TaqII GACCGA 1 cut(s) 1644
TasI AATT 7 cut(s) 1033, 1194, 1278, 1349, 1441, 2089, 2109
TatI WGTACW 5 cut(s) 729, 755, 969, 1096, 1636
TauI GCSGC 1 cut(s) 1474
TfiI GAWTC 4 cut(s) 23, 240, 1591, 2363
Tru1I TTAA 7 cut(s) 711, 1178, 1697, 2025, 2087, 2231, 2399
Tru9I TTAA 7 cut(s) 711, 1178, 1697, 2025, 2087, 2231, 2399
TscAI CASTG 8 cut(s) 796, 1132, 1276, 1665, 1719, 1807, 2043, 2329
TseFI GTSAC 4 cut(s) 72, 923, 1656, 2095
TseI GCWGC 5 cut(s) 452, 857, 1468, 1930, 2248
Tsp45I GTSAC 4 cut(s) 72, 923, 1656, 2095
TspDTI ATGAA 8 cut(s) 729, 934, 1065, 1119, 1362, 1548, 1868, 2028
TspGWI ACGGA 2 cut(s) 587, 1102
TspRI CASTG 8 cut(s) 796, 1132, 1276, 1665, 1719, 1807, 2043, 2329
Van91I CCANNNNNTGG 1 cut(s) 1004
Vha464I CTTAAG 1 cut(s) 1177
VneI GTGCAC 1 cut(s) 1554
VpaK11BI GGWCC 1 cut(s) 800
XapI RAATTY 2 cut(s) 2089, 2109
XceI RCATGY 3 cut(s) 307, 632, 1786
XcmI CCANNNNNNNNNTGG 1 cut(s) 1628
XhoI CTCGAG 1 cut(s) 2316
XmnI GAANNNNTTC 1 cut(s) 282
XspI CTAG 6 cut(s) 456, 725, 1326, 2031, 2100, 2156
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.