pycom14g12880

Squamosa promoter-binding-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0006574
Physical Location & Seq
Forward (+)
15924984 .. 15927243
2260 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1239 bp
ATGGACTGGAACTTGAAAGCAGCTTCTTGGGATTTGTCTGAACTAGAACAAGAAACCTTTTCCAACTTGGACACTACAGTTGATGGGTCTAGCAGCTTTGGAGATCATAGGACCTTCAAAGGAAGCAGCTTTTCTGTTGATTTGAAGCTTGGTCAGTTGGGTGGCTCTGGGAATAATGACCTGGTGGATATGCTGAAGGAGCCCGTTCCGAGAACTACCTCATCATTTTCGTCTGGACCATCAAAGAGGTCTAGAGTGGCTTACAGCGGATCTCAGATGGTTTCCTGCCTCGTTGATGGATGCAATTCGGACCTTAGTACTTGTAGGGATTACCATCGGCGGCATAAGGTCTGTGAGTTACATTCTAAGACTCCTCAGGTCACGATTAACGGAAACAAGCAGCGATTTTGCCAGCAGTGTAGCAGGTTCCATGCTCTGGAGGAATTTGATGAGGGAAAGAGAAGCTGTAGGAAACGTCTTGATGGACACAACCGGCGGCGAAGAAAGCCTCAACCAGAACCCTTGTCACTTCCGGGGAGTTTTATGTCCAGTTACCAAGGTACCCCACGATTGTTGCCACTCTCTAGTTCACTTGTATACCCCTCCACAAATATCGTAAACCCTACCTGGGCCGGCATTGTCAAAACTGAGCCAGATTCTGGGATTCATAACCACCAACTGCCAATGATCGATAAGCAAAACATGTTCCTTGGGTCCTCAGACAGCACCAGCAGCAGCGGTTACAAGGGAGGAAAGCAATTCTCACTCTTCCAAAGCAGCGACAGCTCCCCAGTCCACCACAACCACCAAACATCTCATCATCACAGTGCTTCTGTCTGCCGGCCCCTTCTCAACACCATTTCATTCTCCGAAACTGGTGGTGGTGGTGGCAGTGGGACAACAAAGAGCAAGACGTTTTGTGACAGGTTAACAACTCAAACCCACGATTCGGACTGTGCTCTCTCTCTTCTGTCATCACCGCAGACGCAGCAGAGATCTGAAATAGGTTTGAGACACATGGTGCAGCAGCCTAATTCAATCTCTCTGATGCAACAACGATTTGGCCCGGCGGGTCTACACGGAAACAGCAGTTCCATTGAGCCCATGGATTCAGTTCTGGTCTCCAAAGGAAGTGGTGATGCAAACGTTCATTGTCCCGGAATGTTTCACATTGGCTCTGATGGGTCTCAGGGCAGTGGGAATCAACACCCTCAAACACCTCCATTTCATTGGCAGTAG

Protein Analysis

413

Amino Acids

45.01

Weight (kDa)

8.46

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SBP PF03110 96 - 169 1.2e-31 SBP domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 414
Acc65I GGTACC 1 cut(s) 560
AccB1I GGYRCC 1 cut(s) 560
AccB7I CCANNNNNTGG 2 cut(s) 436, 659
AccI GTMKAC 2 cut(s) 597, 1075
AciI CCGC 6 cut(s) 267, 340, 496, 738, 980, 1070
AclI AACGTT 1 cut(s) 1146
AclWI GGATC 1 cut(s) 277
AcsI RAATTY 1 cut(s) 443
AcuI CTGAAG 1 cut(s) 215
AdeI CACNNNGTG 1 cut(s) 1021
AfaI GTAC 2 cut(s) 319, 562
AfiI CCNNNNNNNGG 4 cut(s) 436, 628, 659, 949
AflIII ACRYGT 1 cut(s) 702
AgsI TTSAA 4 cut(s) 16, 118, 145, 1038
AjnI CCWGG 2 cut(s) 180, 626
AluBI AGCT 6 cut(s) 23, 96, 129, 148, 465, 786
AluI AGCT 6 cut(s) 23, 96, 129, 148, 465, 786
Alw21I GWGCWC 1 cut(s) 961
Alw26I GTCTC 3 cut(s) 1006, 1126, 1191
AlwI GGATC 1 cut(s) 277
AlwNI CAGNNNCTG 1 cut(s) 659
AoxI GGCC 3 cut(s) 630, 842, 1063
ApoI RAATTY 1 cut(s) 443
Asp718I GGTACC 1 cut(s) 560
AspS9I GGNCC 7 cut(s) 111, 236, 310, 630, 714, 843, 1064
AsuC2I CCSGG 3 cut(s) 534, 1067, 1158
AsuHPI GGTGA 2 cut(s) 969, 1148
AvaII GGWCC 4 cut(s) 111, 236, 310, 714
AxyI CCTNAGG 1 cut(s) 375
BaeI ACNNNNGTAYC 2 cut(s) 544, 577
BanI GGYRCC 1 cut(s) 560
BanII GRGCYC 2 cut(s) 204, 1104
Bbv12I GWGCWC 1 cut(s) 961
BccI CCATC 7 cut(s) 77, 247, 271, 290, 342, 476, 1175
BciT130I CCWGG 2 cut(s) 182, 628
BcnI CCSGG 3 cut(s) 534, 1067, 1158
BcoDI GTCTC 3 cut(s) 1006, 1126, 1191
BfaI CTAG 4 cut(s) 44, 90, 252, 585
BfmI CTRYAG 2 cut(s) 75, 466
BfuAI ACCTGC 1 cut(s) 414
BglII AGATCT 1 cut(s) 995
BmcAI AGTACT 1 cut(s) 319
Bme1390I CCNGG 5 cut(s) 182, 534, 628, 1067, 1158
Bme18I GGWCC 4 cut(s) 111, 236, 310, 714
BmgT120I GGNCC 7 cut(s) 111, 236, 310, 630, 714, 843, 1064
BmiI GGNNCC 5 cut(s) 201, 428, 562, 715, 845
BmrFI CCNGG 5 cut(s) 182, 534, 628, 1067, 1158
BmrI ACTGGG 1 cut(s) 785
BmsI GCATC 3 cut(s) 290, 1038, 1129
BmuI ACTGGG 1 cut(s) 785
BpmI CTGGAG 1 cut(s) 458
BpuMI CCSGG 3 cut(s) 534, 1067, 1158
Bsa29I ATCGAT 1 cut(s) 690
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 1126, 1191
BsaJI CCNNGG 5 cut(s) 533, 556, 627, 709, 1104
Bsc4I CCNNNNNNNGG 4 cut(s) 436, 628, 659, 949
Bse118I RCCGGY 3 cut(s) 492, 632, 840
Bse1I ACTGG 4 cut(s) 11, 549, 791, 880
Bse21I CCTNAGG 1 cut(s) 375
Bse8I GATNNNNATC 1 cut(s) 333
BseBI CCWGG 2 cut(s) 182, 628
BseCI ATCGAT 1 cut(s) 690
BseDI CCNNGG 5 cut(s) 533, 556, 627, 709, 1104
BseGI GGATG 1 cut(s) 305
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 4 cut(s) 436, 628, 659, 949
BseMII CTCAG 5 cut(s) 287, 389, 639, 732, 1202
BseNI ACTGG 4 cut(s) 11, 549, 791, 880
BseRI GAGGAG 1 cut(s) 363
BsgI GTGCAG 1 cut(s) 1043
BshFI GGCC 3 cut(s) 632, 844, 1065
BshNI GGYRCC 1 cut(s) 560
BshVI ATCGAT 1 cut(s) 690
BsiHKAI GWGCWC 1 cut(s) 961
BsiSI CCGG 6 cut(s) 493, 533, 633, 841, 1067, 1158
BslFI GGGAC 2 cut(s) 910, 1140
BslI CCNNNNNNNGG 4 cut(s) 436, 628, 659, 949
BsmAI GTCTC 3 cut(s) 1006, 1126, 1191
BsmFI GGGAC 2 cut(s) 910, 1140
BsnI GGCC 3 cut(s) 632, 844, 1065
Bso31I GGTCTC 2 cut(s) 1126, 1191
Bsp1286I GDGCHC 3 cut(s) 204, 961, 1104
Bsp143I GATC 4 cut(s) 103, 269, 687, 995
Bsp19I CCATGG 1 cut(s) 1104
BspACI CCGC 6 cut(s) 267, 340, 496, 738, 980, 1070
BspANI GGCC 3 cut(s) 632, 844, 1065
BspCNI CTCAG 5 cut(s) 286, 388, 640, 731, 1201
BspDI ATCGAT 1 cut(s) 690
BspLI GGNNCC 5 cut(s) 201, 428, 562, 715, 845
BspMI ACCTGC 1 cut(s) 414
BspPI GGATC 1 cut(s) 277
BspT107I GGYRCC 1 cut(s) 560
BspTNI GGTCTC 2 cut(s) 1126, 1191
BsrFI RCCGGY 3 cut(s) 492, 632, 840
BsrI ACTGG 4 cut(s) 11, 549, 791, 880
BssAI RCCGGY 3 cut(s) 492, 632, 840
BssECI CCNNGG 5 cut(s) 533, 556, 627, 709, 1104
BssMI GATC 4 cut(s) 103, 269, 687, 995
BssNAI GTATAC 1 cut(s) 598
BssT1I CCWWGG 3 cut(s) 556, 709, 1104
Bst1107I GTATAC 1 cut(s) 598
Bst2UI CCWGG 2 cut(s) 182, 628
Bst4CI ACNGT 3 cut(s) 79, 827, 956
Bst6I CTCTTC 2 cut(s) 773, 972
BstC8I GCNNGC 3 cut(s) 413, 634, 842
BstDEI CTNAG 7 cut(s) 273, 314, 366, 375, 648, 718, 1188
BstDSI CCRYGG 1 cut(s) 1104
BstF5I GGATG 1 cut(s) 305
BstKTI GATC 4 cut(s) 106, 272, 690, 998
BstMAI GTCTC 3 cut(s) 1006, 1126, 1191
BstMBI GATC 4 cut(s) 103, 269, 687, 995
BstMWI GCNNNNNNNGC 5 cut(s) 199, 505, 732, 783, 988
BstNI CCWGG 2 cut(s) 182, 628
BstNSI RCATGY 1 cut(s) 706
BstSCI CCNGG 5 cut(s) 180, 532, 626, 1065, 1156
BstSFI CTRYAG 2 cut(s) 75, 466
BstX2I RGATCY 2 cut(s) 269, 995
BstXI CCANNNNNNTGG 1 cut(s) 1230
BstYI RGATCY 2 cut(s) 269, 995
BstZ17I GTATAC 1 cut(s) 598
Bsu15I ATCGAT 1 cut(s) 690
Bsu36I CCTNAGG 1 cut(s) 375
BsuRI GGCC 3 cut(s) 632, 844, 1065
BsuTUI ATCGAT 1 cut(s) 690
BtgI CCRYGG 1 cut(s) 1104
BtsCI GGATG 1 cut(s) 305
BtsI GCAGTG 3 cut(s) 422, 898, 1201
BtsIMutI CAGTG 4 cut(s) 422, 832, 898, 1201
BveI ACCTGC 1 cut(s) 414
Cac8I GCNNGC 3 cut(s) 413, 634, 842
CaiI CAGNNNCTG 1 cut(s) 659
Cfr10I RCCGGY 3 cut(s) 492, 632, 840
Cfr13I GGNCC 7 cut(s) 111, 236, 310, 630, 714, 843, 1064
ClaI ATCGAT 1 cut(s) 690
CseI GACGC 1 cut(s) 994
CsiI ACCWGGT 1 cut(s) 180
Csp6I GTAC 2 cut(s) 318, 561
CspCI CAANNNNNGTGG 4 cut(s) 555, 590, 1114, 1149
CviAII CATG 4 cut(s) 431, 703, 1018, 1105
CviQI GTAC 2 cut(s) 318, 561
DdeI CTNAG 7 cut(s) 273, 314, 366, 375, 648, 718, 1188
DpnI GATC 4 cut(s) 105, 271, 689, 997
DpnII GATC 4 cut(s) 103, 269, 687, 995
DraIII CACNNNGTG 1 cut(s) 1021
Eam1104I CTCTTC 2 cut(s) 773, 972
EarI CTCTTC 2 cut(s) 773, 972
Eco130I CCWWGG 3 cut(s) 556, 709, 1104
Eco24I GRGCYC 2 cut(s) 204, 1104
Eco31I GGTCTC 2 cut(s) 1126, 1191
Eco47I GGWCC 4 cut(s) 111, 236, 310, 714
Eco57I CTGAAG 1 cut(s) 215
Eco81I CCTNAGG 1 cut(s) 375
EcoO109I RGGNCCY 2 cut(s) 111, 714
EcoRII CCWGG 2 cut(s) 180, 626
EcoT14I CCWWGG 3 cut(s) 556, 709, 1104
EcoT38I GRGCYC 2 cut(s) 204, 1104
ErhI CCWWGG 3 cut(s) 556, 709, 1104
FaeI CATG 4 cut(s) 434, 706, 1021, 1108
FaqI GGGAC 2 cut(s) 910, 1140
FatI CATG 4 cut(s) 430, 702, 1017, 1104
FauI CCCGC 1 cut(s) 1063
FblI GTMKAC 2 cut(s) 597, 1075
FokI GGATG 1 cut(s) 312
FriOI GRGCYC 2 cut(s) 204, 1104
FspBI CTAG 4 cut(s) 44, 90, 252, 585
GsuI CTGGAG 1 cut(s) 458
HaeIII GGCC 3 cut(s) 632, 844, 1065
HapII CCGG 6 cut(s) 493, 533, 633, 841, 1067, 1158
HgaI GACGC 1 cut(s) 994
Hin1II CATG 4 cut(s) 434, 706, 1021, 1108
HincII GTYRAC 1 cut(s) 930
HindII GTYRAC 1 cut(s) 930
HindIII AAGCTT 1 cut(s) 146
HinfI GANTC 6 cut(s) 370, 656, 664, 947, 1109, 1201
HpaI GTTAAC 1 cut(s) 930
HpaII CCGG 6 cut(s) 493, 533, 633, 841, 1067, 1158
HphI GGTGA 2 cut(s) 969, 1148
Hpy166II GTNNAC 6 cut(s) 590, 598, 619, 796, 930, 1076
Hpy188III TCNNGA 5 cut(s) 234, 252, 382, 437, 479
Hpy8I GTNNAC 6 cut(s) 590, 598, 619, 796, 930, 1076
HpyAV CCTTC 3 cut(s) 124, 190, 857
HpyCH4III ACNGT 3 cut(s) 79, 827, 956
HpyCH4IV ACGT 3 cut(s) 475, 914, 1146
HpyCH4V TGCA 4 cut(s) 303, 1024, 1051, 1142
HpyF10VI GCNNNNNNNGC 5 cut(s) 199, 505, 732, 783, 988
HpyF3I CTNAG 7 cut(s) 273, 314, 366, 375, 648, 718, 1188
HpySE526I ACGT 3 cut(s) 475, 914, 1146
Hsp92II CATG 4 cut(s) 434, 706, 1021, 1108
KpnI GGTACC 1 cut(s) 564
KroI GCCGGC 2 cut(s) 632, 840
KroNI GCCGGC 2 cut(s) 634, 842
KspAI GTTAAC 1 cut(s) 930
Kzo9I GATC 4 cut(s) 103, 269, 687, 995
LmnI GCTCC 2 cut(s) 199, 791
LweI GCATC 3 cut(s) 290, 1038, 1129
MabI ACCWGGT 1 cut(s) 180
MaeI CTAG 4 cut(s) 44, 90, 252, 585
MaeII ACGT 3 cut(s) 475, 914, 1146
MaeIII GTNAC 6 cut(s) 357, 379, 525, 551, 740, 920
MalI GATC 4 cut(s) 105, 271, 689, 997
MboI GATC 4 cut(s) 103, 269, 687, 995
MboII GAAGA 3 cut(s) 513, 760, 959
MflI RGATCY 2 cut(s) 269, 995
MhlI GDGCHC 3 cut(s) 204, 961, 1104
MluCI AATT 4 cut(s) 304, 443, 758, 1033
MlyI GAGTC 1 cut(s) 364
MmeI TCCRAC 1 cut(s) 87
MroNI GCCGGC 2 cut(s) 632, 840
MseI TTAA 2 cut(s) 387, 929
MslI CAYNNNNRTG 1 cut(s) 825
MspA1I CMGCKG 2 cut(s) 267, 738
MspI CCGG 6 cut(s) 493, 533, 633, 841, 1067, 1158
MspR9I CCNGG 5 cut(s) 182, 534, 628, 1067, 1158
MvaI CCWGG 2 cut(s) 182, 628
MwoI GCNNNNNNNGC 5 cut(s) 199, 505, 732, 783, 988
NaeI GCCGGC 2 cut(s) 634, 842
NciI CCSGG 3 cut(s) 534, 1067, 1158
NcoI CCATGG 1 cut(s) 1104
NdeII GATC 4 cut(s) 103, 269, 687, 995
NgoMIV GCCGGC 2 cut(s) 632, 840
NlaIII CATG 4 cut(s) 434, 706, 1021, 1108
NlaIV GGNNCC 5 cut(s) 201, 428, 562, 715, 845
NmuCI GTSAC 3 cut(s) 379, 525, 920
NspI RCATGY 1 cut(s) 706
PciI ACATGT 1 cut(s) 702
PdiI GCCGGC 2 cut(s) 634, 842
PfeI GAWTC 5 cut(s) 656, 664, 947, 1109, 1201
PflMI CCANNNNNTGG 2 cut(s) 436, 659
PfoI TCCNGGA 1 cut(s) 1156
PleI GAGTC 1 cut(s) 364
PpsI GAGTC 1 cut(s) 364
PpuMI RGGWCCY 2 cut(s) 111, 714
PscI ACATGT 1 cut(s) 702
Psp1406I AACGTT 1 cut(s) 1146
Psp5II RGGWCCY 2 cut(s) 111, 714
Psp6I CCWGG 2 cut(s) 180, 626
PspGI CCWGG 2 cut(s) 180, 626
PspN4I GGNNCC 5 cut(s) 201, 428, 562, 715, 845
PspPI GGNCC 7 cut(s) 111, 236, 310, 630, 714, 843, 1064
PspPPI RGGWCCY 2 cut(s) 111, 714
PstNI CAGNNNCTG 1 cut(s) 659
PsuI RGATCY 2 cut(s) 269, 995
RsaI GTAC 2 cut(s) 319, 562
RsaNI GTAC 2 cut(s) 318, 561
RseI CAYNNNNRTG 1 cut(s) 825
SaqAI TTAA 2 cut(s) 387, 929
Sau3AI GATC 4 cut(s) 103, 269, 687, 995
Sau96I GGNCC 7 cut(s) 111, 236, 310, 630, 714, 843, 1064
ScaI AGTACT 1 cut(s) 319
SchI GAGTC 1 cut(s) 364
ScrFI CCNGG 5 cut(s) 182, 534, 628, 1067, 1158
SduI GDGCHC 3 cut(s) 204, 961, 1104
SexAI ACCWGGT 1 cut(s) 180
SfaNI GCATC 3 cut(s) 290, 1038, 1129
SfcI CTRYAG 2 cut(s) 75, 466
SinI GGWCC 4 cut(s) 111, 236, 310, 714
SmiMI CAYNNNNRTG 1 cut(s) 825
Sse9I AATT 4 cut(s) 304, 443, 758, 1033
SsiI CCGC 6 cut(s) 267, 340, 496, 738, 980, 1070
SspMI CTAG 4 cut(s) 44, 90, 252, 585
StyD4I CCNGG 5 cut(s) 180, 532, 626, 1065, 1156
StyI CCWWGG 3 cut(s) 556, 709, 1104
TaaI ACNGT 3 cut(s) 79, 827, 956
TaiI ACGT 3 cut(s) 478, 917, 1149
TaqI TCGA 1 cut(s) 690
TasI AATT 4 cut(s) 304, 443, 758, 1033
TatI WGTACW 1 cut(s) 317
TauI GCSGC 2 cut(s) 343, 499
TfiI GAWTC 5 cut(s) 656, 664, 947, 1109, 1201
Tru1I TTAA 2 cut(s) 387, 929
Tru9I TTAA 2 cut(s) 387, 929
TscAI CASTG 4 cut(s) 422, 832, 898, 1201
TseFI GTSAC 3 cut(s) 379, 525, 920
Tsp45I GTSAC 3 cut(s) 379, 525, 920
TspDTI ATGAA 4 cut(s) 656, 852, 1139, 1217
TspGWI ACGGA 2 cut(s) 405, 1095
TspRI CASTG 4 cut(s) 422, 832, 898, 1201
Van91I CCANNNNNTGG 2 cut(s) 436, 659
VpaK11BI GGWCC 4 cut(s) 111, 236, 310, 714
XapI RAATTY 1 cut(s) 443
XbaI TCTAGA 1 cut(s) 251
XceI RCATGY 1 cut(s) 706
XcmI CCANNNNNNNNNTGG 1 cut(s) 1102
XmiI GTMKAC 2 cut(s) 597, 1075
XspI CTAG 4 cut(s) 44, 90, 252, 585
ZrmI AGTACT 1 cut(s) 319
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.