pycom14g13440

Protein of unknown function (DUF3537)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014739
Physical Location & Seq
Forward (+)
16391125 .. 16397031
5907 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1299 bp
ATGGCAACCCAAATCCCACTGATAGACACATCAGAGCAAAACCCACCTGAAGATGCCAAACTTGATCACTCCCTCCACAGATTTGAGACATTTCTGAGACTCTTTGGCTTCCGCCAGTACTCTTTCTGGAGCTTCATCCTCTCCTGGCTTGCATTTCTCCTTGTGGGTGTTGCAGTTCCTATCTTGCTTACCGAAATCATTGATTGCGCCGACTGCGAAAAGTATGAGATCAAAAGGTTTGAGCTTGAGATTCTGGTTTCTGAGTCTCTGATGGCTGCCATTTCTCTGGGTTGTGTTTCTCACAGCATCAGAAAGTATGGAGTGAGGAAGTTCTTGTTTGTGGATCGGTATCATGGCCTTATGCTTCTGTTTCGTGATGAGTACATTCAAAGGATCAATAAATTCTACCGCTTGCTGGCTGCGTGGGTGTTACCTTGCTTCCTTCTGAAGGCGGCTCGAGAAATCACTCGTGTTCTTGTTCTTCATCACGATCCATGGTGGAAATCTGTTGTCGTACTGCTTGCTGTGCTCGTGTCATGGATGTATTCGAGCATCATTCTCCTATTAGGCAGTGCTTTGTTCAACTTGGTTTGCAATTTGCATATGATTCACTTTGAGAACTATGGAAAACTTTTAGAAAGGGACTTGGATGTTTCTGCATACATTGACGAACATATTCGTCTAACGCATTACCTATCGAAGATCAGCCACAGATTCCGAATCTATCTTCTTCTTCAGCTCTTAGTTGTGACAGCAAGCCAGTTTGTGGCTCTACTGCAGACAACATCAAAGCACGGGACCATCAACCTCATCAATGGCGGTGATTTTGTAGTCTGTTCCATTGTTGAGGTAGGTGGGCTAGTTATATGCCTGCATGCAGCTTCGAAGATTTCACACAGAGCTCAGGCGCTTGGATCGGTGACTAGCAGATGGCATGCTATGGTCACTTGCAGCTCCAAAGATGCGCCTCGACGGGGAACCAATAATAGCTCCGGAAACTTGGGAATCACCAACAGTTCCGGGAACTTGGAGATTTCTATTGCTGCTTCACTGCCGATAAGCTACTCAGAAAGTGATCTGGAGGCAGTTGATTATGTTCCAAAGCCTACAAATACACAATTGGCCTCACACATGTCCCTCTATCAGAAAAGACAATCTTTTGTGACCTACATGCAGTCCAACCCTACGGGAATTACCCTCTATGGATGGACGATTGACCGGGGACTCGTTAGCACTATCTGCTTCATTGAGGTCACACTGGCTCTTTTCGTGCTCGGGAAGACGATAACGTTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3537

Protein Analysis

433

Amino Acids

48.86

Weight (kDa)

8.3

Isoelectric Point (pI)

30.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3537 PF12056 24 - 416 1e-142 Protein of unknown function (DUF3537)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014739)

Species Orthologous Gene IDs
fragaria_vesca FvH4_5g07730
malus_domestica MD14G1161500.v1.1
prunus_persica Prupe.5G154400_v2.0.a1
pyrus_communis pycom14g13440
rosa_chinensis RchiOBHm_Chr7g0190691
rosa_laevigata RLG00000004503
rosa_multiflora Rmu_sc0006709.1_g000002 Rmu_sc0014053.1_g000002 Rmu_sc0014053.1_g000003 Rmu_sc0026963.1_g000001
rosa_roxburghii Rroxscaffold_3G00264400
rosa_rugosa Rorug06G0506500
rosa_samantha Rh7AG113000 Rh7BG115500 Rh7CG118000 Rh7DG116900
rosa_wichuraiana Rw7G009860

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 766
AccIII TCCGGA 1 cut(s) 992
AciI CCGC 4 cut(s) 112, 409, 452, 819
AclI AACGTT 1 cut(s) 1289
AclWI GGATC 4 cut(s) 351, 401, 485, 922
AcsI RAATTY 1 cut(s) 401
AcuI CTGAAG 3 cut(s) 69, 467, 719
AfaI GTAC 3 cut(s) 119, 383, 516
AfiI CCNNNNNNNGG 4 cut(s) 415, 448, 766, 974
AflIII ACRYGT 1 cut(s) 1131
AgsI TTSAA 2 cut(s) 389, 583
AjnI CCWGG 1 cut(s) 143
AluBI AGCT 8 cut(s) 132, 244, 739, 881, 902, 954, 990, 1062
AluI AGCT 8 cut(s) 132, 244, 739, 881, 902, 954, 990, 1062
Alw21I GWGCWC 3 cut(s) 531, 904, 1275
Alw26I GTCTC 3 cut(s) 80, 91, 270
AlwI GGATC 4 cut(s) 351, 401, 485, 922
Ama87I CYCGRG 2 cut(s) 456, 1274
Aor13HI TCCGGA 1 cut(s) 992
AoxI GGCC 2 cut(s) 355, 1122
ApeKI GCWGC 5 cut(s) 275, 419, 878, 951, 1043
ApoI RAATTY 1 cut(s) 401
Asp700I GAANNNNTTC 1 cut(s) 675
AspLEI GCGC 3 cut(s) 209, 910, 967
AspS9I GGNCC 1 cut(s) 798
AsuC2I CCSGG 2 cut(s) 1021, 1220
AsuHPI GGTGA 3 cut(s) 833, 931, 1000
AsuII TTCGAA 1 cut(s) 884
AvaI CYCGRG 2 cut(s) 456, 1274
AvaII GGWCC 1 cut(s) 798
BanII GRGCYC 1 cut(s) 904
BauI CACGAG 2 cut(s) 468, 530
BbsI GAAGAC 1 cut(s) 1286
Bbv12I GWGCWC 3 cut(s) 531, 904, 1275
BbvI GCAGC 5 cut(s) 262, 406, 890, 963, 1030
BccI CCATC 4 cut(s) 265, 809, 924, 1200
BciT130I CCWGG 1 cut(s) 145
BclI TGATCA 1 cut(s) 64
BcnI CCSGG 2 cut(s) 1021, 1220
BcoDI GTCTC 3 cut(s) 80, 91, 270
BfaI CTAG 2 cut(s) 860, 924
BfmI CTRYAG 1 cut(s) 776
BfoI RGCGCY 1 cut(s) 911
BisI GCNGC 6 cut(s) 276, 420, 453, 879, 952, 1044
BlsI GCNGC 6 cut(s) 277, 421, 454, 880, 953, 1045
BmcAI AGTACT 1 cut(s) 119
Bme1390I CCNGG 3 cut(s) 145, 1021, 1220
Bme18I GGWCC 1 cut(s) 798
BmeT110I CYCGRG 2 cut(s) 456, 1274
BmgT120I GGNCC 1 cut(s) 798
BmiI GGNNCC 2 cut(s) 799, 979
BmrFI CCNGG 3 cut(s) 145, 1021, 1220
BmsI GCATC 4 cut(s) 43, 315, 561, 952
BpiI GAAGAC 1 cut(s) 1286
BpmI CTGGAG 2 cut(s) 148, 1100
Bpu10I CCTNAGC 1 cut(s) 903
Bpu14I TTCGAA 1 cut(s) 884
BpuEI CTTGAG 1 cut(s) 266
BpuMI CCSGG 2 cut(s) 1021, 1220
BsaBI GATNNNNATC 1 cut(s) 348
BsaJI CCNNGG 2 cut(s) 494, 1219
BsaWI WCCGGW 1 cut(s) 992
Bsc4I CCNNNNNNNGG 4 cut(s) 415, 448, 766, 974
Bse1I ACTGG 3 cut(s) 115, 760, 1263
Bse8I GATNNNNATC 1 cut(s) 348
BseAI TCCGGA 1 cut(s) 992
BseBI CCWGG 1 cut(s) 145
BseDI CCNNGG 2 cut(s) 494, 1219
BseGI GGATG 4 cut(s) 135, 546, 655, 1211
BseJI GATNNNNATC 1 cut(s) 348
BseLI CCNNNNNNNGG 4 cut(s) 415, 448, 766, 974
BseMII CTCAG 4 cut(s) 86, 252, 917, 1080
BseNI ACTGG 3 cut(s) 115, 760, 1263
BseXI GCAGC 5 cut(s) 262, 406, 890, 963, 1030
BshFI GGCC 2 cut(s) 357, 1124
BsiHKAI GWGCWC 3 cut(s) 531, 904, 1275
BsiHKCI CYCGRG 2 cut(s) 456, 1274
BsiSI CCGG 3 cut(s) 993, 1020, 1219
BslFI GGGAC 4 cut(s) 656, 811, 1120, 1236
BslI CCNNNNNNNGG 4 cut(s) 415, 448, 766, 974
BsmAI GTCTC 3 cut(s) 80, 91, 270
BsmFI GGGAC 4 cut(s) 656, 811, 1120, 1236
BsnI GGCC 2 cut(s) 357, 1124
BsoBI CYCGRG 2 cut(s) 456, 1274
Bsp119I TTCGAA 1 cut(s) 884
Bsp1286I GDGCHC 3 cut(s) 531, 904, 1275
Bsp13I TCCGGA 1 cut(s) 992
Bsp143I GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
Bsp19I CCATGG 1 cut(s) 494
BspACI CCGC 4 cut(s) 112, 409, 452, 819
BspANI GGCC 2 cut(s) 357, 1124
BspCNI CTCAG 4 cut(s) 87, 253, 916, 1079
BspEI TCCGGA 1 cut(s) 992
BspLI GGNNCC 2 cut(s) 799, 979
BspMAI CTGCAG 1 cut(s) 780
BspPI GGATC 4 cut(s) 351, 401, 485, 922
BspT104I TTCGAA 1 cut(s) 884
BsrI ACTGG 3 cut(s) 115, 760, 1263
BssECI CCNNGG 2 cut(s) 494, 1219
BssMI GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
BssSI CACGAG 2 cut(s) 468, 530
BssT1I CCWWGG 1 cut(s) 494
Bst2BI CACGAG 2 cut(s) 468, 530
Bst2UI CCWGG 1 cut(s) 145
Bst4CI ACNGT 1 cut(s) 1016
BstAPI GCANNNNNTGC 1 cut(s) 1239
BstBI TTCGAA 1 cut(s) 884
BstC8I GCNNGC 8 cut(s) 150, 413, 417, 522, 757, 872, 876, 936
BstDEI CTNAG 5 cut(s) 95, 261, 742, 903, 1066
BstDSI CCRYGG 1 cut(s) 494
BstENI CCTNNNNNAGG 1 cut(s) 446
BstF5I GGATG 4 cut(s) 135, 546, 655, 1211
BstH2I RGCGCY 1 cut(s) 911
BstHHI GCGC 3 cut(s) 209, 910, 967
BstKTI GATC 8 cut(s) 67, 231, 346, 396, 493, 705, 917, 1078
BstMAI GTCTC 3 cut(s) 80, 91, 270
BstMBI GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
BstMWI GCNNNNNNNGC 3 cut(s) 213, 526, 1239
BstNI CCWGG 1 cut(s) 145
BstNSI RCATGY 4 cut(s) 878, 938, 1135, 1174
BstSCI CCNGG 3 cut(s) 143, 1019, 1218
BstSFI CTRYAG 1 cut(s) 776
BstV1I GCAGC 5 cut(s) 262, 406, 890, 963, 1030
BstV2I GAAGAC 1 cut(s) 1286
BstXI CCANNNNNNTGG 1 cut(s) 286
BsuRI GGCC 2 cut(s) 357, 1124
BtgI CCRYGG 1 cut(s) 494
BtsCI GGATG 4 cut(s) 135, 546, 655, 1211
BtsI GCAGTG 2 cut(s) 577, 1049
BtsIMutI CAGTG 4 cut(s) 17, 577, 1049, 1256
Cac8I GCNNGC 8 cut(s) 150, 413, 417, 522, 757, 872, 876, 936
CfoI GCGC 3 cut(s) 209, 910, 967
Cfr13I GGNCC 1 cut(s) 798
Csp6I GTAC 3 cut(s) 118, 382, 515
CviAII CATG 7 cut(s) 353, 495, 537, 875, 935, 1132, 1171
CviQI GTAC 3 cut(s) 118, 382, 515
DdeI CTNAG 5 cut(s) 95, 261, 742, 903, 1066
DpnI GATC 8 cut(s) 66, 230, 345, 395, 492, 704, 916, 1077
DpnII GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
EciI GGCGGA 1 cut(s) 101
Ecl136II GAGCTC 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 494
Eco24I GRGCYC 1 cut(s) 904
Eco47I GGWCC 1 cut(s) 798
Eco53kI GAGCTC 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 69, 467, 719
Eco88I CYCGRG 2 cut(s) 456, 1274
EcoICRI GAGCTC 1 cut(s) 902
EcoNI CCTNNNNNAGG 1 cut(s) 446
EcoRII CCWGG 1 cut(s) 143
EcoT14I CCWWGG 1 cut(s) 494
EcoT38I GRGCYC 1 cut(s) 904
ErhI CCWWGG 1 cut(s) 494
FaeI CATG 7 cut(s) 356, 498, 540, 878, 938, 1135, 1174
FalI AAGNNNNNCTT 2 cut(s) 1141, 1173
FaqI GGGAC 4 cut(s) 656, 811, 1120, 1236
FatI CATG 7 cut(s) 352, 494, 536, 874, 934, 1131, 1170
FauNDI CATATG 1 cut(s) 603
FbaI TGATCA 1 cut(s) 64
Fnu4HI GCNGC 6 cut(s) 276, 420, 453, 879, 952, 1044
FokI GGATG 4 cut(s) 122, 553, 662, 1218
FriOI GRGCYC 1 cut(s) 904
Fsp4HI GCNGC 6 cut(s) 276, 420, 453, 879, 952, 1044
FspBI CTAG 2 cut(s) 860, 924
GlaI GCGC 3 cut(s) 208, 909, 966
GluI GCNGC 6 cut(s) 276, 420, 453, 879, 952, 1044
GsuI CTGGAG 2 cut(s) 148, 1100
HaeII RGCGCY 1 cut(s) 911
HaeIII GGCC 2 cut(s) 357, 1124
HapII CCGG 3 cut(s) 993, 1020, 1219
HhaI GCGC 3 cut(s) 209, 910, 967
Hin1II CATG 7 cut(s) 356, 498, 540, 878, 938, 1135, 1174
Hin6I GCGC 3 cut(s) 207, 908, 965
HinP1I GCGC 3 cut(s) 207, 908, 965
HinfI GANTC 8 cut(s) 99, 250, 263, 607, 714, 720, 1005, 1224
HpaII CCGG 3 cut(s) 993, 1020, 1219
HphI GGTGA 3 cut(s) 833, 931, 1000
Hpy166II GTNNAC 1 cut(s) 1293
Hpy188I TCNGA 9 cut(s) 34, 96, 262, 270, 311, 447, 719, 1069, 1146
Hpy188III TCNNGA 7 cut(s) 127, 374, 458, 488, 993, 1079, 1276
Hpy8I GTNNAC 1 cut(s) 1293
Hpy99I CGWCG 1 cut(s) 975
HpyAV CCTTC 2 cut(s) 442, 452
HpyCH4III ACNGT 1 cut(s) 1016
HpyCH4IV ACGT 1 cut(s) 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 213, 526, 1239
HpyF3I CTNAG 5 cut(s) 95, 261, 742, 903, 1066
HpySE526I ACGT 1 cut(s) 1289
Hsp92II CATG 7 cut(s) 356, 498, 540, 878, 938, 1135, 1174
HspAI GCGC 3 cut(s) 207, 908, 965
Kpn2I TCCGGA 1 cut(s) 992
Ksp22I TGATCA 1 cut(s) 64
Kzo9I GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
LmnI GCTCC 3 cut(s) 129, 959, 995
Lsp1109I GCAGC 5 cut(s) 262, 406, 890, 963, 1030
LweI GCATC 4 cut(s) 43, 315, 561, 952
MaeI CTAG 2 cut(s) 860, 924
MaeII ACGT 1 cut(s) 1289
MaeIII GTNAC 6 cut(s) 429, 748, 919, 943, 1162, 1252
MalI GATC 8 cut(s) 66, 230, 345, 395, 492, 704, 916, 1077
MboI GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
MboII GAAGA 8 cut(s) 62, 473, 712, 719, 722, 725, 898, 1291
MfeI CAATTG 1 cut(s) 1118
MhlI GDGCHC 3 cut(s) 531, 904, 1275
MluCI AATT 4 cut(s) 401, 595, 1118, 1191
MlyI GAGTC 3 cut(s) 93, 272, 1218
MmeI TCCRAC 1 cut(s) 1203
MroI TCCGGA 1 cut(s) 992
MroXI GAANNNNTTC 1 cut(s) 675
MspI CCGG 3 cut(s) 993, 1020, 1219
MspR9I CCNGG 3 cut(s) 145, 1021, 1220
MunI CAATTG 1 cut(s) 1118
MvaI CCWGG 1 cut(s) 145
MwoI GCNNNNNNNGC 3 cut(s) 213, 526, 1239
NciI CCSGG 2 cut(s) 1021, 1220
NcoI CCATGG 1 cut(s) 494
NdeI CATATG 1 cut(s) 603
NdeII GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
NlaIII CATG 7 cut(s) 356, 498, 540, 878, 938, 1135, 1174
NlaIV GGNNCC 2 cut(s) 799, 979
NmuCI GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
NspI RCATGY 4 cut(s) 878, 938, 1135, 1174
NspV TTCGAA 1 cut(s) 884
PaeI GCATGC 2 cut(s) 878, 938
PaeR7I CTCGAG 1 cut(s) 456
PciI ACATGT 1 cut(s) 1131
PdmI GAANNNNTTC 1 cut(s) 675
PfeI GAWTC 5 cut(s) 250, 607, 714, 720, 1005
PflMI CCANNNNNTGG 1 cut(s) 766
PfoI TCCNGGA 1 cut(s) 1019
PkrI GCNGC 6 cut(s) 277, 421, 454, 880, 953, 1045
PleI GAGTC 3 cut(s) 93, 271, 1218
PpsI GAGTC 3 cut(s) 93, 271, 1218
PscI ACATGT 1 cut(s) 1131
Psp124BI GAGCTC 1 cut(s) 904
Psp1406I AACGTT 1 cut(s) 1289
Psp6I CCWGG 1 cut(s) 143
PspGI CCWGG 1 cut(s) 143
PspN4I GGNNCC 2 cut(s) 799, 979
PspPI GGNCC 1 cut(s) 798
PstI CTGCAG 1 cut(s) 780
RsaI GTAC 3 cut(s) 119, 383, 516
RsaNI GTAC 3 cut(s) 118, 382, 515
SacI GAGCTC 1 cut(s) 904
SatI GCNGC 6 cut(s) 276, 420, 453, 879, 952, 1044
Sau3AI GATC 8 cut(s) 64, 228, 343, 393, 490, 702, 914, 1075
Sau96I GGNCC 1 cut(s) 798
ScaI AGTACT 1 cut(s) 119
SchI GAGTC 3 cut(s) 93, 272, 1218
ScrFI CCNGG 3 cut(s) 145, 1021, 1220
SduI GDGCHC 3 cut(s) 531, 904, 1275
SfaNI GCATC 4 cut(s) 43, 315, 561, 952
SfcI CTRYAG 1 cut(s) 776
Sfr274I CTCGAG 1 cut(s) 456
SfuI TTCGAA 1 cut(s) 884
SinI GGWCC 1 cut(s) 798
SlaI CTCGAG 1 cut(s) 456
SmlI CTYRAG 2 cut(s) 245, 456
SmoI CTYRAG 2 cut(s) 245, 456
SphI GCATGC 2 cut(s) 878, 938
Sse9I AATT 4 cut(s) 401, 595, 1118, 1191
SsiI CCGC 4 cut(s) 112, 409, 452, 819
SspMI CTAG 2 cut(s) 860, 924
SstI GAGCTC 1 cut(s) 904
StyD4I CCNGG 3 cut(s) 143, 1019, 1218
StyI CCWWGG 1 cut(s) 494
TaaI ACNGT 1 cut(s) 1016
TaiI ACGT 1 cut(s) 1292
TaqI TCGA 5 cut(s) 457, 548, 698, 884, 970
TasI AATT 4 cut(s) 401, 595, 1118, 1191
TatI WGTACW 2 cut(s) 117, 381
TauI GCSGC 1 cut(s) 455
TfiI GAWTC 5 cut(s) 250, 607, 714, 720, 1005
TscAI CASTG 4 cut(s) 24, 577, 1056, 1263
TseFI GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
TseI GCWGC 5 cut(s) 275, 419, 878, 951, 1043
Tsp45I GTSAC 5 cut(s) 748, 919, 943, 1162, 1252
TspDTI ATGAA 3 cut(s) 124, 473, 1234
TspRI CASTG 4 cut(s) 24, 577, 1056, 1263
Van91I CCANNNNNTGG 1 cut(s) 766
VpaK11BI GGWCC 1 cut(s) 798
XagI CCTNNNNNAGG 1 cut(s) 446
XapI RAATTY 1 cut(s) 401
XceI RCATGY 4 cut(s) 878, 938, 1135, 1174
XhoI CTCGAG 1 cut(s) 456
XmnI GAANNNNTTC 1 cut(s) 675
XspI CTAG 2 cut(s) 860, 924
ZrmI AGTACT 1 cut(s) 119
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.