pycom14g16670

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0014174
Physical Location & Seq
Forward (+)
18879279 .. 18880974
1696 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1185 bp
ATGCACCACACAGTTTCTAGTCTTCGATTTCACATCGGAAGGTCTAAAAACACCTGCATTTCTCGAGTTTTTTCAGCCATGAAGTTCACAACGTTGGCAGCAATGATGGTGGCCGTGGCCGTCTTCGGCCTCCTCATCGATGGCCACGACTGCAAAATCGTGCAGTTTATATTCGGGGACTCCCTCTCCGACATCGGAAACAACATGTACTTGAGCAAGAGTATCGCCCAAGCAAACCTGCCTTGGTATGGCATTGATATGGGCAATGGACTGCCTAATGGAAGGTTCTCTAATGGCCGTACCGTTGCTGATATAATAGGTGATAACATGGGGCTCCCACGGCCACCGGCCTTTCTCGATCCATCTTTAAATGAAGACATGATACTCGAAAACGGAGTGAACTACGCCTCTGGAGGTGGTGGGATTTTGAATGAAACCGGTGGCTACTTTATTCAGAGGCTTTCGCTCTACAAGCAGATCGAGCTTTTTCAAGGAACACAAACACTTATCAGAAGCAAAATCGGTGAACAAGCAGCGGAGAAGTTCTTCCAAGACGCTCGTTATGTGGTTGCTTTAGGCAGCAACGACTTCATTAACAACTATTTGATGCCTGTTTACAGCGATTCGTGGAGTTACAACGATCAAACTTTCATGGAATACTTAATGGGAACGCTAGAGAAGCAACTTAAGCTGTTGCACAGCCTAGGGGCGAGGCAGCTGATGGTGTTCGGGCTAGGACCGATGGGTTGTATTCCACTCCAAAGGGTGCTAAGTACATCTTCGGCTTGCCAAGATAGAACAAACAAACTAGCTCTCAGCTTCAACAAAGCTGGTAGCAAGCTTATGGAAGATTTGTCCAGCCAGCTTCCTAACGCGAGCTATCGATTTGGTGACGCGTATGATGTTGTCAACAATGTGATCAGCGATCCAAACAAATACGGATTCAGCAATGCGGACTCACCATGCTGCTCATTTGGAAGAATTCGACCGGCTTTGACGTGCGTCCCTGCATCAGTACTGTGCAAAGACAGAAGCAAATATGTGTTTTGGGACGAGTACCATCCATCGGACAGCGCAAACAAGCTTATTGCCACGGAACTCATAAAGAAATTCGGTTTCTCGCGCGTTGGTGAAACAGATGGTCCTTCCCCTGCACCTGCCATTGTTCAATCACCAGAGGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

395

Amino Acids

43.27

Weight (kDa)

6.6

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 56 - 366 2.8e-30 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0014174)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G74460
fragaria_vesca FvH4_5g14860
malus_domestica MD06G1194000.v1.1 MD14G1201200.v1.1
prunus_persica Prupe.5G194400_v2.0.a1
pyrus_communis pycom06g17190 pycom14g16670
rosa_chinensis RchiOBHm_Chr7g0181521
rosa_laevigata RLG00000005186
rosa_multiflora Rmu_sc0004621.1_g000032
rosa_roxburghii Rroxscaffold_3G00271930
rosa_rugosa Rorug06G0442000
rosa_samantha Rh7AG044100 Rh7BG043200 Rh7CG045000 Rh7DG043700
rosa_wichuraiana Rw7G003650

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 62, 1165
Acc36I ACCTGC 3 cut(s) 62, 246, 1165
AccII CGCG 4 cut(s) 875, 896, 1123, 1125
AciI CCGC 2 cut(s) 536, 953
AclI AACGTT 1 cut(s) 92
AclWI GGATC 2 cut(s) 353, 920
AcoI YGGCCR 5 cut(s) 111, 117, 142, 295, 341
AcsI RAATTY 2 cut(s) 981, 1109
AfaI GTAC 5 cut(s) 209, 301, 775, 1017, 1058
AfiI CCNNNNNNNGG 2 cut(s) 248, 1066
AflII CTTAAG 1 cut(s) 686
AflIII ACRYGT 2 cut(s) 204, 894
AgeI ACCGGT 1 cut(s) 437
AgsI TTSAA 4 cut(s) 430, 491, 823, 1169
AjiI CACGTC 1 cut(s) 999
AlwI GGATC 2 cut(s) 353, 920
Ama87I CYCGRG 1 cut(s) 63
AoxI GGCC 7 cut(s) 111, 117, 127, 142, 295, 341, 348
ApeKI GCWGC 5 cut(s) 98, 533, 579, 715, 966
ApoI RAATTY 2 cut(s) 981, 1109
AsiGI ACCGGT 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 545
AspA2I CCTAGG 1 cut(s) 703
AspLEI GCGC 2 cut(s) 1076, 1125
AspS9I GGNCC 2 cut(s) 737, 1142
AsuHPI GGTGA 6 cut(s) 332, 536, 902, 951, 1142, 1164
AvaI CYCGRG 1 cut(s) 63
AvaII GGWCC 2 cut(s) 737, 1142
AvrII CCTAGG 1 cut(s) 703
BalI TGGCCA 1 cut(s) 144
BanII GRGCYC 1 cut(s) 336
BarI GAAGNNNNNNTAC 2 cut(s) 366, 398
BbsI GAAGAC 3 cut(s) 14, 115, 381
BbvI GCAGC 5 cut(s) 110, 545, 591, 727, 953
BccI CCATC 8 cut(s) 100, 134, 370, 715, 736, 1068, 1072, 1133
BceAI ACGGC 4 cut(s) 98, 104, 282, 356
BcgI CGANNNNNNTGC 2 cut(s) 205, 239
BclI TGATCA 1 cut(s) 918
BfaI CTAG 5 cut(s) 18, 674, 704, 734, 809
BfrI CTTAAG 1 cut(s) 686
BfuAI ACCTGC 3 cut(s) 62, 246, 1165
BisI GCNGC 5 cut(s) 99, 534, 580, 716, 967
BlnI CCTAGG 1 cut(s) 703
BlsI GCNGC 5 cut(s) 100, 535, 581, 717, 968
BmcAI AGTACT 1 cut(s) 1017
Bme18I GGWCC 2 cut(s) 737, 1142
BmeT110I CYCGRG 1 cut(s) 63
BmgBI CACGTC 1 cut(s) 999
BmgT120I GGNCC 2 cut(s) 737, 1142
BmiI GGNNCC 1 cut(s) 335
BmsI GCATC 2 cut(s) 597, 1019
BoxI GACNNNNGTC 1 cut(s) 1001
BpiI GAAGAC 3 cut(s) 14, 115, 381
BpmI CTGGAG 1 cut(s) 432
BpuEI CTTGAG 1 cut(s) 232
Bsa29I ATCGAT 2 cut(s) 138, 883
BsaJI CCNNGG 5 cut(s) 114, 242, 338, 703, 1092
BsaWI WCCGGW 1 cut(s) 437
BsaXI ACNNNNNCTCC 2 cut(s) 170, 200
Bsc4I CCNNNNNNNGG 2 cut(s) 248, 1066
Bse118I RCCGGY 3 cut(s) 346, 437, 988
Bse3DI GCAATG 3 cut(s) 108, 271, 955
BseCI ATCGAT 2 cut(s) 138, 883
BseDI CCNNGG 5 cut(s) 114, 242, 338, 703, 1092
BseGI GGATG 1 cut(s) 1060
BseLI CCNNNNNNNGG 2 cut(s) 248, 1066
BseMI GCAATG 3 cut(s) 108, 271, 955
BseMII CTCAG 1 cut(s) 829
BseRI GAGGAG 1 cut(s) 122
BseXI GCAGC 5 cut(s) 110, 545, 591, 727, 953
BsgI GTGCAG 2 cut(s) 182, 1137
Bsh1236I CGCG 4 cut(s) 875, 896, 1123, 1125
Bsh1285I CGRYCG 1 cut(s) 989
BshFI GGCC 7 cut(s) 113, 119, 129, 144, 297, 343, 350
BshTI ACCGGT 1 cut(s) 437
BshVI ATCGAT 2 cut(s) 138, 883
BsiEI CGRYCG 1 cut(s) 989
BsiHKCI CYCGRG 1 cut(s) 63
BsiSI CCGG 3 cut(s) 347, 438, 989
BslFI GGGAC 3 cut(s) 191, 989, 1064
BslI CCNNNNNNNGG 2 cut(s) 248, 1066
BsmFI GGGAC 3 cut(s) 191, 989, 1064
BsnI GGCC 7 cut(s) 113, 119, 129, 144, 297, 343, 350
BsoBI CYCGRG 1 cut(s) 63
Bsp1286I GDGCHC 1 cut(s) 336
Bsp143I GATC 5 cut(s) 358, 477, 640, 918, 925
BspACI CCGC 2 cut(s) 536, 953
BspANI GGCC 7 cut(s) 113, 119, 129, 144, 297, 343, 350
BspCNI CTCAG 1 cut(s) 828
BspDI ATCGAT 2 cut(s) 138, 883
BspFNI CGCG 4 cut(s) 875, 896, 1123, 1125
BspLI GGNNCC 1 cut(s) 335
BspMI ACCTGC 3 cut(s) 62, 246, 1165
BspPI GGATC 2 cut(s) 353, 920
BspTI CTTAAG 1 cut(s) 686
BsrDI GCAATG 3 cut(s) 108, 271, 955
BsrFI RCCGGY 3 cut(s) 346, 437, 988
BssAI RCCGGY 3 cut(s) 346, 437, 988
BssECI CCNNGG 5 cut(s) 114, 242, 338, 703, 1092
BssMI GATC 5 cut(s) 358, 477, 640, 918, 925
BssT1I CCWWGG 2 cut(s) 242, 703
Bst4CI ACNGT 3 cut(s) 13, 304, 1020
BstAFI CTTAAG 1 cut(s) 686
BstC8I GCNNGC 4 cut(s) 787, 839, 863, 877
BstDEI CTNAG 2 cut(s) 770, 815
BstDSI CCRYGG 3 cut(s) 114, 338, 1092
BstF5I GGATG 1 cut(s) 1060
BstFNI CGCG 4 cut(s) 875, 896, 1123, 1125
BstHHI GCGC 2 cut(s) 1076, 1125
BstKTI GATC 5 cut(s) 361, 480, 643, 921, 928
BstMBI GATC 5 cut(s) 358, 477, 640, 918, 925
BstMCI CGRYCG 1 cut(s) 989
BstMWI GCNNNNNNNGC 6 cut(s) 150, 340, 472, 481, 679, 688
BstNSI RCATGY 1 cut(s) 208
BstPAI GACNNNNGTC 1 cut(s) 1001
BstUI CGCG 4 cut(s) 875, 896, 1123, 1125
BstV1I GCAGC 5 cut(s) 110, 545, 591, 727, 953
BstV2I GAAGAC 3 cut(s) 14, 115, 381
Bsu15I ATCGAT 2 cut(s) 138, 883
BsuRI GGCC 7 cut(s) 113, 119, 129, 144, 297, 343, 350
BsuTUI ATCGAT 2 cut(s) 138, 883
BtgI CCRYGG 3 cut(s) 114, 338, 1092
BtrI CACGTC 1 cut(s) 999
BtsCI GGATG 1 cut(s) 1060
BveI ACCTGC 3 cut(s) 62, 246, 1165
Cac8I GCNNGC 4 cut(s) 787, 839, 863, 877
CfoI GCGC 2 cut(s) 1076, 1125
Cfr10I RCCGGY 3 cut(s) 346, 437, 988
Cfr13I GGNCC 2 cut(s) 737, 1142
ClaI ATCGAT 2 cut(s) 138, 883
CseI GACGC 3 cut(s) 563, 902, 991
Csp6I GTAC 5 cut(s) 208, 300, 774, 1016, 1057
CspAI ACCGGT 1 cut(s) 437
CspCI CAANNNNNGTGG 2 cut(s) 90, 125
CviAII CATG 6 cut(s) 79, 205, 328, 379, 652, 963
CviQI GTAC 5 cut(s) 208, 300, 774, 1016, 1057
DdeI CTNAG 2 cut(s) 770, 815
DpnI GATC 5 cut(s) 360, 479, 642, 920, 927
DpnII GATC 5 cut(s) 358, 477, 640, 918, 925
DraI TTTAAA 1 cut(s) 369
EaeI YGGCCR 5 cut(s) 111, 117, 142, 295, 341
Eco130I CCWWGG 2 cut(s) 242, 703
Eco24I GRGCYC 1 cut(s) 336
Eco47I GGWCC 2 cut(s) 737, 1142
Eco88I CYCGRG 1 cut(s) 63
EcoRI GAATTC 1 cut(s) 981
EcoT14I CCWWGG 2 cut(s) 242, 703
EcoT38I GRGCYC 1 cut(s) 336
ErhI CCWWGG 2 cut(s) 242, 703
FaeI CATG 6 cut(s) 82, 208, 331, 382, 655, 966
FalI AAGNNNNNCTT 2 cut(s) 763, 795
FaqI GGGAC 3 cut(s) 191, 989, 1064
FatI CATG 6 cut(s) 78, 204, 327, 378, 651, 962
FbaI TGATCA 1 cut(s) 918
Fnu4HI GCNGC 5 cut(s) 99, 534, 580, 716, 967
FokI GGATG 1 cut(s) 1047
FriOI GRGCYC 1 cut(s) 336
Fsp4HI GCNGC 5 cut(s) 99, 534, 580, 716, 967
FspBI CTAG 5 cut(s) 18, 674, 704, 734, 809
GlaI GCGC 2 cut(s) 1075, 1124
GluI GCNGC 5 cut(s) 99, 534, 580, 716, 967
GsuI CTGGAG 1 cut(s) 432
HaeIII GGCC 7 cut(s) 113, 119, 129, 144, 297, 343, 350
HapII CCGG 3 cut(s) 347, 438, 989
HgaI GACGC 3 cut(s) 563, 902, 991
HhaI GCGC 2 cut(s) 1076, 1125
Hin1II CATG 6 cut(s) 82, 208, 331, 382, 655, 966
Hin6I GCGC 2 cut(s) 1074, 1123
HinP1I GCGC 2 cut(s) 1074, 1123
HincII GTYRAC 1 cut(s) 910
HindII GTYRAC 1 cut(s) 910
HindIII AAGCTT 2 cut(s) 839, 1082
HinfI GANTC 4 cut(s) 179, 623, 942, 956
HpaII CCGG 3 cut(s) 347, 438, 989
HphI GGTGA 6 cut(s) 332, 536, 902, 951, 1142, 1164
Hpy166II GTNNAC 5 cut(s) 87, 400, 527, 616, 910
Hpy188I TCNGA 6 cut(s) 38, 190, 197, 456, 512, 1069
Hpy188III TCNNGA 3 cut(s) 63, 356, 411
Hpy8I GTNNAC 5 cut(s) 87, 400, 527, 616, 910
HpyAV CCTTC 3 cut(s) 33, 276, 1155
HpyCH4III ACNGT 3 cut(s) 13, 304, 1020
HpyCH4IV ACGT 2 cut(s) 92, 998
HpyCH4V TGCA 8 cut(s) 4, 57, 153, 163, 697, 1010, 1023, 1154
HpyF10VI GCNNNNNNNGC 6 cut(s) 150, 340, 472, 481, 679, 688
HpyF3I CTNAG 2 cut(s) 770, 815
HpySE526I ACGT 2 cut(s) 92, 998
Hsp92II CATG 6 cut(s) 82, 208, 331, 382, 655, 966
HspAI GCGC 2 cut(s) 1074, 1123
Ksp22I TGATCA 1 cut(s) 918
Kzo9I GATC 5 cut(s) 358, 477, 640, 918, 925
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 5 cut(s) 110, 545, 591, 727, 953
LweI GCATC 2 cut(s) 597, 1019
MaeI CTAG 5 cut(s) 18, 674, 704, 734, 809
MaeII ACGT 2 cut(s) 92, 998
MaeIII GTNAC 2 cut(s) 632, 890
MalI GATC 5 cut(s) 360, 479, 642, 920, 927
MboI GATC 5 cut(s) 358, 477, 640, 918, 925
MboII GAAGA 7 cut(s) 14, 115, 386, 538, 771, 860, 990
MhlI GDGCHC 1 cut(s) 336
MlsI TGGCCA 1 cut(s) 144
MluCI AATT 2 cut(s) 981, 1109
MluI ACGCGT 1 cut(s) 894
MluNI TGGCCA 1 cut(s) 144
MlyI GAGTC 2 cut(s) 173, 950
MmeI TCCRAC 1 cut(s) 213
MnlI CCTC 8 cut(s) 140, 143, 194, 407, 418, 450, 705, 1171
Mox20I TGGCCA 1 cut(s) 144
MroXI GAANNNNTTC 1 cut(s) 545
MscI TGGCCA 1 cut(s) 144
MseI TTAA 4 cut(s) 368, 594, 662, 687
MslI CAYNNNNRTG 1 cut(s) 257
Msp20I TGGCCA 1 cut(s) 144
MspA1I CMGCKG 2 cut(s) 536, 718
MspCI CTTAAG 1 cut(s) 686
MspI CCGG 3 cut(s) 347, 438, 989
MvnI CGCG 4 cut(s) 875, 896, 1123, 1125
MwoI GCNNNNNNNGC 6 cut(s) 150, 340, 472, 481, 679, 688
NdeII GATC 5 cut(s) 358, 477, 640, 918, 925
NlaIII CATG 6 cut(s) 82, 208, 331, 382, 655, 966
NlaIV GGNNCC 1 cut(s) 335
NmuCI GTSAC 1 cut(s) 890
NspI RCATGY 1 cut(s) 208
PaeR7I CTCGAG 1 cut(s) 63
PaqCI CACCTGC 2 cut(s) 62, 1165
PciI ACATGT 1 cut(s) 204
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PdmI GAANNNNTTC 1 cut(s) 545
PfeI GAWTC 2 cut(s) 623, 942
PinAI ACCGGT 1 cut(s) 437
PkrI GCNGC 5 cut(s) 100, 535, 581, 717, 968
PleI GAGTC 2 cut(s) 173, 950
PpsI GAGTC 2 cut(s) 173, 950
PscI ACATGT 1 cut(s) 204
PshAI GACNNNNGTC 1 cut(s) 1001
Psp1406I AACGTT 1 cut(s) 92
PspN4I GGNNCC 1 cut(s) 335
PspPI GGNCC 2 cut(s) 737, 1142
PvuII CAGCTG 1 cut(s) 718
RsaI GTAC 5 cut(s) 209, 301, 775, 1017, 1058
RsaNI GTAC 5 cut(s) 208, 300, 774, 1016, 1057
RseI CAYNNNNRTG 1 cut(s) 257
SaqAI TTAA 4 cut(s) 368, 594, 662, 687
SatI GCNGC 5 cut(s) 99, 534, 580, 716, 967
Sau3AI GATC 5 cut(s) 358, 477, 640, 918, 925
Sau96I GGNCC 2 cut(s) 737, 1142
ScaI AGTACT 1 cut(s) 1017
SchI GAGTC 2 cut(s) 173, 950
SduI GDGCHC 1 cut(s) 336
SfaNI GCATC 2 cut(s) 597, 1019
Sfr274I CTCGAG 1 cut(s) 63
SinI GGWCC 2 cut(s) 737, 1142
SlaI CTCGAG 1 cut(s) 63
SmiMI CAYNNNNRTG 1 cut(s) 257
SmlI CTYRAG 3 cut(s) 63, 211, 686
SmoI CTYRAG 3 cut(s) 63, 211, 686
Sse9I AATT 2 cut(s) 981, 1109
SsiI CCGC 2 cut(s) 536, 953
SspMI CTAG 5 cut(s) 18, 674, 704, 734, 809
StyI CCWWGG 2 cut(s) 242, 703
TaaI ACNGT 3 cut(s) 13, 304, 1020
TaiI ACGT 2 cut(s) 95, 1001
TaqI TCGA 8 cut(s) 25, 64, 138, 357, 387, 480, 883, 985
TaqII GACCGA 1 cut(s) 754
TasI AATT 2 cut(s) 981, 1109
TatI WGTACW 3 cut(s) 207, 773, 1015
TfiI GAWTC 2 cut(s) 623, 942
Tru1I TTAA 4 cut(s) 368, 594, 662, 687
Tru9I TTAA 4 cut(s) 368, 594, 662, 687
TseFI GTSAC 1 cut(s) 890
TseI GCWGC 5 cut(s) 98, 533, 579, 715, 966
Tsp45I GTSAC 1 cut(s) 890
TspDTI ATGAA 5 cut(s) 95, 387, 447, 580, 640
TspGWI ACGGA 3 cut(s) 408, 954, 1109
Vha464I CTTAAG 1 cut(s) 686
VpaK11BI GGWCC 2 cut(s) 737, 1142
XapI RAATTY 2 cut(s) 981, 1109
XceI RCATGY 1 cut(s) 208
XhoI CTCGAG 1 cut(s) 63
XmaJI CCTAGG 1 cut(s) 703
XmnI GAANNNNTTC 1 cut(s) 545
XspI CTAG 5 cut(s) 18, 674, 704, 734, 809
ZrmI AGTACT 1 cut(s) 1017
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.