pycom14g16710

calcium-binding protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0016496
Physical Location & Seq
Forward (+)
18906468 .. 18907106
639 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 639 bp
ATGGACAAGGCGATGAGCGATGGGTTCGGATGCAGCCCTGCTTCGCTTCTTGCACTTCTCTTAGTCCTAATCATTCTTAACTGGCTCCTTGTACTGCACTTCAACTCGAATTTACGCTACTTGTTTTTATGCTTCCTTTGCGATGTTATGCAGATTAAGCCCGTGATCCAAAGAAGTTGCACTGACGAGAACGTATGTTATCACAAACCATCCGAAGGAGTTGAAATTAACTCTATAACTACAAGTCCTGATCAAGTCCCTTCAGTTCAGGCCGCGGAGATTAGTTACATTCACGAAAATGTTGCAGATGGCGAGAAGAATCTGTGTGTAGGAGAACTAGGACTAGTGATGGAGACACTAGGAACATTAAGTGAGGGGAGACTCGGGTCGAAGGAGATTGCTGATTTGTTTGAGGAGGACCCGAGCTTGGAGGAAGTAAAAGAAGCGTTTCGCGTGTTTGATGAGAACGAGGACGGCTTCATTGATGCAGGTGAGCTAATGAAGGTTCTCTCTGTATTGGGTTTCGTTGAAGCTTCGGAAATGGAATGCAAACGCATGATCAAGGCATTTGATGTTGACGGAGATGGACGCATAGATTTCGATGAGTTTGTTAAACTCATGGAAAACAGCTTCTGCTGA

Protein Analysis

213

Amino Acids

23.61

Weight (kDa)

4.27

Isoelectric Point (pI)

38.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EF-hand_7 PF13499 144 - 207 1.4e-15 EF-hand domain pair
EF-hand_1 PF00036 145 - 171 2.6e-06 EF hand domain
EF-hand_6 PF13405 145 - 174 9.3e-07 EF-hand domain
EF-hand_8 PF13833 157 - 209 3.5e-09 EF-hand domain pair
EF-hand_1 PF00036 182 - 208 3.7e-09 EF hand domain
EF-hand_5 PF13202 187 - 207 5.8e-06 EF hand
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016496)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47480
fragaria_vesca FvH4_5g14810
malus_domestica MD06G1194700.v1.1 MD14G1201700.v1.1
prunus_persica Prupe.5G194800_v2.0.a1
pyrus_communis pycom14g16710
rosa_chinensis RchiOBHm_Chr7g0181581
rosa_multiflora Rmu_sc0000988.1_g000019
rosa_roxburghii Rroxscaffold_3G00271880
rosa_samantha Rh7AG044600 Rh7BG043600 Rh7CG045400 Rh7DG044200
rosa_wichuraiana Rw7G003700

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 479
Acc36I ACCTGC 1 cut(s) 479
AccII CGCG 2 cut(s) 275, 453
AciI CCGC 2 cut(s) 273, 275
AclWI GGATC 1 cut(s) 160
AcsI RAATTY 1 cut(s) 109
AcuI CTGAAG 1 cut(s) 246
AfaI GTAC 1 cut(s) 93
AfiI CCNNNNNNNGG 2 cut(s) 215, 427
AgsI TTSAA 3 cut(s) 103, 224, 530
AhlI ACTAGT 1 cut(s) 343
AluBI AGCT 4 cut(s) 426, 496, 533, 630
AluI AGCT 4 cut(s) 426, 496, 533, 630
Alw26I GTCTC 2 cut(s) 347, 373
AlwI GGATC 1 cut(s) 160
AlwNI CAGNNNCTG 1 cut(s) 633
Ama87I CYCGRG 2 cut(s) 383, 421
AoxI GGCC 1 cut(s) 270
ApeKI GCWGC 1 cut(s) 33
ApoI RAATTY 1 cut(s) 109
Asp700I GAANNNNTTC 1 cut(s) 447
AspS9I GGNCC 1 cut(s) 418
AsuHPI GGTGA 1 cut(s) 503
AvaI CYCGRG 2 cut(s) 383, 421
AvaII GGWCC 1 cut(s) 418
BbvI GCAGC 1 cut(s) 45
BccI CCATC 5 cut(s) 14, 217, 302, 343, 578
BceAI ACGGC 1 cut(s) 490
BcgI CGANNNNNNTGC 4 cut(s) 284, 318, 580, 614
BclI TGATCA 2 cut(s) 250, 558
BcoDI GTCTC 2 cut(s) 347, 373
BcuI ACTAGT 1 cut(s) 343
BfaI CTAG 3 cut(s) 338, 344, 359
BfuAI ACCTGC 1 cut(s) 479
BisI GCNGC 2 cut(s) 34, 273
BlsI GCNGC 2 cut(s) 35, 274
Bme18I GGWCC 1 cut(s) 418
BmeT110I CYCGRG 2 cut(s) 383, 421
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 2 cut(s) 86, 420
BmsI GCATC 2 cut(s) 20, 475
BoxI GACNNNNGTC 1 cut(s) 385
BsaJI CCNNGG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 427
Bse1I ACTGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 273
BseGI GGATG 2 cut(s) 35, 209
BseLI CCNNNNNNNGG 2 cut(s) 215, 427
BseNI ACTGG 1 cut(s) 86
BseRI GAGGAG 1 cut(s) 428
BseXI GCAGC 1 cut(s) 45
BsgI GTGCAG 1 cut(s) 80
Bsh1236I CGCG 2 cut(s) 275, 453
BshFI GGCC 1 cut(s) 272
BsiHKCI CYCGRG 2 cut(s) 383, 421
BslFI GGGAC 1 cut(s) 242
BslI CCNNNNNNNGG 2 cut(s) 215, 427
BsmAI GTCTC 2 cut(s) 347, 373
BsmFI GGGAC 1 cut(s) 242
BsmI GAATGC 1 cut(s) 551
BsnI GGCC 1 cut(s) 272
BsoBI CYCGRG 2 cut(s) 383, 421
Bsp143I GATC 3 cut(s) 165, 250, 558
BspACI CCGC 2 cut(s) 273, 275
BspANI GGCC 1 cut(s) 272
BspFNI CGCG 2 cut(s) 275, 453
BspLI GGNNCC 2 cut(s) 86, 420
BspMI ACCTGC 1 cut(s) 479
BspPI GGATC 1 cut(s) 160
BsrI ACTGG 1 cut(s) 86
BssECI CCNNGG 1 cut(s) 273
BssMI GATC 3 cut(s) 165, 250, 558
BstDEI CTNAG 1 cut(s) 61
BstDSI CCRYGG 1 cut(s) 273
BstF5I GGATG 2 cut(s) 35, 209
BstFNI CGCG 2 cut(s) 275, 453
BstKTI GATC 3 cut(s) 168, 253, 561
BstMAI GTCTC 2 cut(s) 347, 373
BstMBI GATC 3 cut(s) 165, 250, 558
BstMWI GCNNNNNNNGC 2 cut(s) 138, 157
BstPAI GACNNNNGTC 1 cut(s) 385
BstUI CGCG 2 cut(s) 275, 453
BstV1I GCAGC 1 cut(s) 45
BsuRI GGCC 1 cut(s) 272
BtgI CCRYGG 1 cut(s) 273
BtgZI GCGATG 3 cut(s) 26, 33, 156
BtsCI GGATG 2 cut(s) 35, 209
BtsIMutI CAGTG 1 cut(s) 180
BveI ACCTGC 1 cut(s) 479
CaiI CAGNNNCTG 1 cut(s) 633
Cfr13I GGNCC 1 cut(s) 418
Cfr42I CCGCGG 1 cut(s) 276
CseI GACGC 1 cut(s) 597
Csp6I GTAC 1 cut(s) 92
CviAII CATG 2 cut(s) 556, 619
CviJI RGCY 9 cut(s) 36, 85, 160, 272, 426, 477, 496, 533, 630
CviKI_1 RGCY 9 cut(s) 36, 85, 160, 272, 426, 477, 496, 533, 630
CviQI GTAC 1 cut(s) 92
DdeI CTNAG 1 cut(s) 61
DpnI GATC 3 cut(s) 167, 252, 560
DpnII GATC 3 cut(s) 165, 250, 558
Eco47I GGWCC 1 cut(s) 418
Eco57I CTGAAG 1 cut(s) 246
Eco88I CYCGRG 2 cut(s) 383, 421
EcoO109I RGGNCCY 1 cut(s) 418
FaeI CATG 2 cut(s) 559, 622
FaiI YATR 7 cut(s) 130, 149, 196, 236, 557, 593, 620
FaqI GGGAC 1 cut(s) 242
FatI CATG 2 cut(s) 555, 618
FbaI TGATCA 2 cut(s) 250, 558
Fnu4HI GCNGC 2 cut(s) 34, 273
FokI GGATG 2 cut(s) 42, 196
Fsp4HI GCNGC 2 cut(s) 34, 273
FspBI CTAG 3 cut(s) 338, 344, 359
GluI GCNGC 2 cut(s) 34, 273
HaeIII GGCC 1 cut(s) 272
HgaI GACGC 1 cut(s) 597
Hin1II CATG 2 cut(s) 559, 622
HincII GTYRAC 1 cut(s) 577
HindII GTYRAC 1 cut(s) 577
HindIII AAGCTT 1 cut(s) 531
HinfI GANTC 2 cut(s) 319, 381
HphI GGTGA 1 cut(s) 503
Hpy166II GTNNAC 1 cut(s) 577
Hpy188I TCNGA 3 cut(s) 29, 214, 538
Hpy188III TCNNGA 2 cut(s) 248, 293
Hpy8I GTNNAC 1 cut(s) 577
HpyAV CCTTC 4 cut(s) 209, 270, 385, 496
HpyCH4IV ACGT 1 cut(s) 192
HpyCH4V TGCA 8 cut(s) 33, 53, 97, 151, 180, 305, 488, 549
HpyF10VI GCNNNNNNNGC 2 cut(s) 138, 157
HpyF3I CTNAG 1 cut(s) 61
HpySE526I ACGT 1 cut(s) 192
Hsp92II CATG 2 cut(s) 559, 622
Ksp22I TGATCA 2 cut(s) 250, 558
KspI CCGCGG 1 cut(s) 276
Kzo9I GATC 3 cut(s) 165, 250, 558
LmnI GCTCC 1 cut(s) 90
LpnPI CCDG 5 cut(s) 51, 67, 254, 261, 474
Lsp1109I GCAGC 1 cut(s) 45
LweI GCATC 2 cut(s) 20, 475
MaeI CTAG 3 cut(s) 338, 344, 359
MaeII ACGT 1 cut(s) 192
MaeIII GTNAC 1 cut(s) 284
MalI GATC 3 cut(s) 167, 252, 560
MboI GATC 3 cut(s) 165, 250, 558
MboII GAAGA 1 cut(s) 328
MluCI AATT 2 cut(s) 109, 225
MlyI GAGTC 1 cut(s) 375
MnlI CCTC 5 cut(s) 367, 406, 409, 424, 463
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 5 cut(s) 78, 156, 228, 368, 612
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 275
Mva1269I GAATGC 1 cut(s) 551
MvnI CGCG 2 cut(s) 275, 453
MwoI GCNNNNNNNGC 2 cut(s) 138, 157
NdeII GATC 3 cut(s) 165, 250, 558
NlaIII CATG 2 cut(s) 559, 622
NlaIV GGNNCC 2 cut(s) 86, 420
PaqCI CACCTGC 1 cut(s) 479
PctI GAATGC 1 cut(s) 551
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 319
PkrI GCNGC 2 cut(s) 35, 274
PleI GAGTC 1 cut(s) 375
PpsI GAGTC 1 cut(s) 375
PpuMI RGGWCCY 1 cut(s) 418
PshAI GACNNNNGTC 1 cut(s) 385
Psp5II RGGWCCY 1 cut(s) 418
PspN4I GGNNCC 2 cut(s) 86, 420
PspPI GGNCC 1 cut(s) 418
PspPPI RGGWCCY 1 cut(s) 418
PstNI CAGNNNCTG 1 cut(s) 633
RsaI GTAC 1 cut(s) 93
RsaNI GTAC 1 cut(s) 92
RseI CAYNNNNRTG 1 cut(s) 297
SacII CCGCGG 1 cut(s) 276
SaqAI TTAA 5 cut(s) 78, 156, 228, 368, 612
SatI GCNGC 2 cut(s) 34, 273
Sau3AI GATC 3 cut(s) 165, 250, 558
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 1 cut(s) 375
SetI ASST 7 cut(s) 195, 428, 493, 498, 507, 535, 632
SfaNI GCATC 2 cut(s) 20, 475
Sfr303I CCGCGG 1 cut(s) 276
SgrBI CCGCGG 1 cut(s) 276
SinI GGWCC 1 cut(s) 418
SmiMI CAYNNNNRTG 1 cut(s) 297
SpeI ACTAGT 1 cut(s) 343
Sse9I AATT 2 cut(s) 109, 225
SsiI CCGC 2 cut(s) 273, 275
SspMI CTAG 3 cut(s) 338, 344, 359
TaiI ACGT 1 cut(s) 195
TaqI TCGA 3 cut(s) 107, 389, 600
TasI AATT 2 cut(s) 109, 225
TatI WGTACW 1 cut(s) 91
TauI GCSGC 1 cut(s) 275
TfiI GAWTC 1 cut(s) 319
Tru1I TTAA 5 cut(s) 78, 156, 228, 368, 612
Tru9I TTAA 5 cut(s) 78, 156, 228, 368, 612
TscAI CASTG 1 cut(s) 187
TseI GCWGC 1 cut(s) 33
TspDTI ATGAA 2 cut(s) 469, 515
TspGWI ACGGA 1 cut(s) 594
TspRI CASTG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 418
XapI RAATTY 1 cut(s) 109
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 3 cut(s) 338, 344, 359
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.