pycom14g18020

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0010318
Physical Location & Seq
Reverse (-)
19818183 .. 19820929
2747 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 618 bp
ATGGATTCGGTAGAAAAAGGATCCGGGGATCCCCTTCTTAACTATTCCTCAAAAACACTGCAAAGCCACGATTTTTTTTCACTTGGCTGTGGACTTTGTTATAGTTTCTTAGCTGTTAGCAACAGCAAAATTGTGAGCATGGCGAGCTTGCCGTCTCCGTCGTCTCCGGCGTCGCCATCCTCCTCTGCTTCTAGCTCCGACAACTCCGCCACTCGACCCATCTCTCACTCTCCTCCAAATCCACAGGTTGAAGCTGGAGCCAACAATGGAGTTCTGGATGCCGAGGAGAAGAAGCCTGGTATTGTTTCTGCATATTATGATGACCTCCATAGCGCAAACCACATTGAGAAGTTCAAGAAATATGAAGTGGACTACAGTCGTTGGTTGACTGCAAAATACTTCTCAAAGACGAACCTATATGGAGGCAACATCTTTGATGAGAGTGTGACAATACATGATCAGGTTATAAAGTCGAGCAGATGGCCTTGCACCCTCTCATATGCAGACCCGGTCCAGGGTTTTGAAGAACAGAGCAACATCTTTTCAACTTTTACCACTACAGCAGAAACCTCGCCTAACGTCTCAAATGGGAAGCATCAAGTAAAGAAAAGCGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

206

Amino Acids

22.25

Weight (kDa)

5.65

Isoelectric Point (pI)

60.24

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 467
AciI CCGC 1 cut(s) 207
AclWI GGATC 4 cut(s) 15, 23, 28, 36
AcyI GRCGYC 1 cut(s) 170
AfiI CCNNNNNNNGG 2 cut(s) 514, 515
AgsI TTSAA 4 cut(s) 251, 355, 524, 546
AjnI CCWGG 2 cut(s) 295, 513
AluBI AGCT 4 cut(s) 113, 147, 195, 254
AluI AGCT 4 cut(s) 113, 147, 195, 254
Alw26I GTCTC 3 cut(s) 159, 168, 586
AlwI GGATC 4 cut(s) 15, 23, 28, 36
AoxI GGCC 1 cut(s) 482
AspLEI GCGC 1 cut(s) 335
AspS9I GGNCC 1 cut(s) 511
AsuC2I CCSGG 2 cut(s) 25, 509
AvaII GGWCC 1 cut(s) 511
BamHI GGATCC 2 cut(s) 20, 28
BccI CCATC 3 cut(s) 184, 227, 474
BceAI ACGGC 1 cut(s) 136
BcgI CGANNNNNNTGC 2 cut(s) 552, 586
BciT130I CCWGG 2 cut(s) 297, 515
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 2 cut(s) 25, 509
BcoDI GTCTC 3 cut(s) 159, 168, 586
BfaI CTAG 1 cut(s) 192
BfmI CTRYAG 2 cut(s) 373, 558
Bme1390I CCNGG 4 cut(s) 25, 297, 509, 515
Bme18I GGWCC 1 cut(s) 511
BmgT120I GGNCC 1 cut(s) 511
BmiI GGNNCC 3 cut(s) 22, 30, 259
BmrFI CCNGG 4 cut(s) 25, 297, 509, 515
BmsI GCATC 2 cut(s) 268, 604
BoxI GACNNNNGTC 1 cut(s) 375
BpmI CTGGAG 1 cut(s) 276
BpuMI CCSGG 2 cut(s) 25, 509
BsaHI GRCGYC 1 cut(s) 170
BsaJI CCNNGG 3 cut(s) 24, 282, 514
Bsc4I CCNNNNNNNGG 2 cut(s) 514, 515
BseBI CCWGG 2 cut(s) 297, 515
BseDI CCNNGG 3 cut(s) 24, 282, 514
BseGI GGATG 2 cut(s) 176, 283
BseLI CCNNNNNNNGG 2 cut(s) 514, 515
BseRI GAGGAG 3 cut(s) 172, 222, 299
BshFI GGCC 1 cut(s) 484
BsiSI CCGG 3 cut(s) 24, 167, 509
BslI CCNNNNNNNGG 2 cut(s) 514, 515
BsmAI GTCTC 3 cut(s) 159, 168, 586
BsmBI CGTCTC 3 cut(s) 159, 168, 586
BsnI GGCC 1 cut(s) 484
Bsp143I GATC 3 cut(s) 20, 28, 457
BspACI CCGC 1 cut(s) 207
BspANI GGCC 1 cut(s) 484
BspLI GGNNCC 3 cut(s) 22, 30, 259
BspPI GGATC 4 cut(s) 15, 23, 28, 36
BssECI CCNNGG 3 cut(s) 24, 282, 514
BssMI GATC 3 cut(s) 20, 28, 457
BssNI GRCGYC 1 cut(s) 170
Bst2UI CCWGG 2 cut(s) 297, 515
Bst4CI ACNGT 1 cut(s) 377
BstACI GRCGYC 1 cut(s) 170
BstC8I GCNNGC 2 cut(s) 145, 149
BstDEI CTNAG 1 cut(s) 109
BstF5I GGATG 2 cut(s) 176, 283
BstHHI GCGC 1 cut(s) 335
BstKTI GATC 3 cut(s) 23, 31, 460
BstMAI GTCTC 3 cut(s) 159, 168, 586
BstMBI GATC 3 cut(s) 20, 28, 457
BstMWI GCNNNNNNNGC 1 cut(s) 144
BstNI CCWGG 2 cut(s) 297, 515
BstPAI GACNNNNGTC 1 cut(s) 375
BstSCI CCNGG 4 cut(s) 23, 295, 507, 513
BstSFI CTRYAG 2 cut(s) 373, 558
BstX2I RGATCY 2 cut(s) 20, 28
BstYI RGATCY 2 cut(s) 20, 28
BsuRI GGCC 1 cut(s) 484
BtsCI GGATG 2 cut(s) 176, 283
BtsI GCAGTG 1 cut(s) 56
BtsIMutI CAGTG 1 cut(s) 56
Cac8I GCNNGC 2 cut(s) 145, 149
CfoI GCGC 1 cut(s) 335
Cfr13I GGNCC 1 cut(s) 511
CseI GACGC 1 cut(s) 159
CviAII CATG 2 cut(s) 139, 455
CviJI RGCY 9 cut(s) 66, 87, 113, 147, 195, 254, 260, 295, 484
CviKI_1 RGCY 9 cut(s) 66, 87, 113, 147, 195, 254, 260, 295, 484
DdeI CTNAG 1 cut(s) 109
DpnI GATC 3 cut(s) 22, 30, 459
DpnII GATC 3 cut(s) 20, 28, 457
EciI GGCGGA 1 cut(s) 196
Eco47I GGWCC 1 cut(s) 511
EcoRII CCWGG 2 cut(s) 295, 513
Esp3I CGTCTC 3 cut(s) 159, 168, 586
FaeI CATG 2 cut(s) 142, 458
FatI CATG 2 cut(s) 138, 454
FauNDI CATATG 1 cut(s) 499
FbaI TGATCA 1 cut(s) 457
FokI GGATG 2 cut(s) 163, 290
FspBI CTAG 1 cut(s) 192
GlaI GCGC 1 cut(s) 334
GsuI CTGGAG 1 cut(s) 276
HaeIII GGCC 1 cut(s) 484
HapII CCGG 3 cut(s) 24, 167, 509
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 335
Hin1I GRCGYC 1 cut(s) 170
Hin1II CATG 2 cut(s) 142, 458
Hin6I GCGC 1 cut(s) 333
HinP1I GCGC 1 cut(s) 333
HincII GTYRAC 1 cut(s) 387
HindII GTYRAC 1 cut(s) 387
HinfI GANTC 1 cut(s) 5
HpaII CCGG 3 cut(s) 24, 167, 509
Hpy166II GTNNAC 3 cut(s) 92, 370, 387
Hpy188I TCNGA 1 cut(s) 199
Hpy188III TCNNGA 2 cut(s) 275, 355
Hpy8I GTNNAC 3 cut(s) 92, 370, 387
Hpy99I CGWCG 2 cut(s) 163, 175
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 377
HpyCH4IV ACGT 1 cut(s) 579
HpyCH4V TGCA 5 cut(s) 61, 311, 392, 489, 503
HpyF10VI GCNNNNNNNGC 1 cut(s) 144
HpyF3I CTNAG 1 cut(s) 109
HpySE526I ACGT 1 cut(s) 579
Hsp92I GRCGYC 1 cut(s) 170
Hsp92II CATG 2 cut(s) 142, 458
HspAI GCGC 1 cut(s) 333
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 3 cut(s) 20, 28, 457
LmnI GCTCC 2 cut(s) 200, 257
LweI GCATC 2 cut(s) 268, 604
MaeI CTAG 1 cut(s) 192
MaeII ACGT 1 cut(s) 579
MaeIII GTNAC 1 cut(s) 445
MalI GATC 3 cut(s) 22, 30, 459
MboI GATC 3 cut(s) 20, 28, 457
MboII GAAGA 2 cut(s) 301, 536
MflI RGATCY 2 cut(s) 20, 28
MluCI AATT 1 cut(s) 129
MmeI TCCRAC 1 cut(s) 222
MnlI CCTC 9 cut(s) 58, 190, 193, 243, 277, 335, 416, 503, 580
MseI TTAA 1 cut(s) 39
MspI CCGG 3 cut(s) 24, 167, 509
MspR9I CCNGG 4 cut(s) 25, 297, 509, 515
MvaI CCWGG 2 cut(s) 297, 515
MwoI GCNNNNNNNGC 1 cut(s) 144
NciI CCSGG 2 cut(s) 25, 509
NdeI CATATG 1 cut(s) 499
NdeII GATC 3 cut(s) 20, 28, 457
NlaIII CATG 2 cut(s) 142, 458
NlaIV GGNNCC 3 cut(s) 22, 30, 259
NmeAIII GCCGAG 1 cut(s) 307
NmuCI GTSAC 1 cut(s) 445
PcsI WCGNNNNNNNCGW 1 cut(s) 167
PfeI GAWTC 1 cut(s) 5
PflFI GACNNNGTC 1 cut(s) 509
PshAI GACNNNNGTC 1 cut(s) 375
PsiI TTATAA 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 295, 513
PspGI CCWGG 2 cut(s) 295, 513
PspN4I GGNNCC 3 cut(s) 22, 30, 259
PspPI GGNCC 1 cut(s) 511
PsuI RGATCY 2 cut(s) 20, 28
PsyI GACNNNGTC 1 cut(s) 509
SaqAI TTAA 1 cut(s) 39
Sau3AI GATC 3 cut(s) 20, 28, 457
Sau96I GGNCC 1 cut(s) 511
ScrFI CCNGG 4 cut(s) 25, 297, 509, 515
SfaNI GCATC 2 cut(s) 268, 604
SfcI CTRYAG 2 cut(s) 373, 558
SinI GGWCC 1 cut(s) 511
Sse9I AATT 1 cut(s) 129
SsiI CCGC 1 cut(s) 207
SspMI CTAG 1 cut(s) 192
StyD4I CCNGG 4 cut(s) 23, 295, 507, 513
TaaI ACNGT 1 cut(s) 377
TaiI ACGT 1 cut(s) 582
TaqI TCGA 2 cut(s) 214, 473
TasI AATT 1 cut(s) 129
TfiI GAWTC 1 cut(s) 5
Tru1I TTAA 1 cut(s) 39
Tru9I TTAA 1 cut(s) 39
TscAI CASTG 1 cut(s) 63
TseFI GTSAC 1 cut(s) 445
Tsp45I GTSAC 1 cut(s) 445
TspDTI ATGAA 1 cut(s) 378
TspGWI ACGGA 1 cut(s) 147
TspRI CASTG 1 cut(s) 63
Tth111I GACNNNGTC 1 cut(s) 509
VpaK11BI GGWCC 1 cut(s) 511
XspI CTAG 1 cut(s) 192
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.