Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr14

Orthogroup ID

OG0007797

Physical Location & Seq

Genomic Coordinates Reverse (-)

20165467 .. 20174112

Gene Structure 8646 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2820 bp

ATGGCTCGGATCTTCGAGCACTTCGTGGTGTGCGGGATCGGACCCGAGATTCGGACCCTCGACGGCGCCAAAGGATTCCACGGTTTCGACACATATTACCTGCCGTCGCTTCTGGACCAGTACCCTCCGCCCAACCACACTCTCTACCCTCCTCCTCCGCCTCAGCTCCCCACCTGTGTGTTGCCAGCCGGTGTTTTGTTCTATTCGTCGGGCTTTGATTCCAATGACGCCTATACGTTTCCACGGAGCTACCCCATTGTTCTCACTGAGGGTGATGGGACAAAAATTTATGTTAGTTGCATTGCTTTCCGGGATCCAGTCAGTGAGGATATTGCTGAAGCTTACTGTATACCTGCAAATTCATTTGCTGATAAATGTATCTGTCTTGTTTCACGGTCACCCAGCTTTCGTTTACTTAAGAATGCACTGGAGGAATTATTTGCACTTTGTTTTTCCCCTAATGGAAGTAGCAAACCCTTATGGGAAGTTATTGCATCTTTGGTGTCCACTGTACCTTTGCCTACTCCAGGAAAAGACCGAGTTCTGTTTGCTATTGACAATTGTCTACTTTCGGTGGAGGCTCCACCAAAGGATGGGCTCCCTCATGTTGATATATCGTTTCAGCCATTGGTACAGTGCCTAGATGTTGACAACTTGATTAAATTTTTCACCGCTGTGTTGCTTGAAAGGAGAATACTGATCCGATCAAACAAATATTCACTTTTGACCCTTGTATCCGAAGCCATTTGCCATTTACTTTATCCATTGCGGTGGCAGCAAGTCTATATTCCGTTACTCTTTTTCAGTGGAGTAGACTATATTGATGCGCCTACGCCATATATGATGGGTCTCCATTCAGGTGTTGATACGTCTACTTTGGCCATGGATGGTGTAGTTGTTGTTGACCTTGAGTGTAATCGTATCACAACAACGGAAGATATACCTCCAATCCCAGAGCAAGAATTGGGCAATCTGCGTGGAGAGATATCAAAGCTATTGTATCCCAATGTTGTGGGGATAGATCAGATCAAGGATGGTTTATATAGTTCATCTGAGCAATATTCTAAAGCTGGCAATAAACCTTGGGGAGAGGGTCACGACCTTCAACTTAGGTTGGTGTTCTTAAAGTTCTTTGCATCACTTTTAAGTGGCTACCGCAACTTCATAGAAATTTCTGCTACTCATGTCTTCAACACTCAAGCATTCTTGGCAATGCGCTCTCGGTCAACTGGCCAACCACCAGAACCCATGTTAATGCAGTTTCTGGATTCGCATGGTTTCATGGATTATCTAGAGAGATGTTTGGGTTCCGAAGAAAACACTAATAATCTACTTGACAAGTTACAAGATGCACTTAGAAGGGGCCAAAATCCTATTTCAATTCTTCCCTCAAATTCAGTAGAGCCTGAGATTATAACAATTGCAGATACGGATGTGGGAAATTCAGGATCAGGTACCAAATACACATATGATAGGTTTCCCTCAGACCTTAGGACTGAAGAGCAAGAAGAAAAGAGGAAGCAGATCCTGGCTGCAGCAAGTGGATTATCCTCTAGCTCACAATCTGTTCCAGGGGGTAAAGACTCTAAATCAGAAACCCTAACTCCAATGGAGAGGGCTGCGGAAAGGGAGTGCATGGTTTTGGACATAAAAGTTAAGCTGCAGGGTCTGTGGCTTCGTCTTCTGAAATTGGGACCTACTGATGATCCTCTTTCTTCATTTGAATATGGCACAATCCTCGCTTTAATTGAATCTGATGCTGAGGGAATTGGTGGCAGTGGGTTTGTTGAATGTATAAGGGAACACATAAATCAGGGATGGCACTGTCAGTTGACTGAAGGACAATTTCTTGCTGTTAAGGAACTGCTCAAAACAGCTATTAGTCGTGCAACTTCCCGAAATGATATGTTGACAATCAGGGATGCCCTTGAAGTATCCGCTGACATGTATAAGAAGGATGCACATAATGTTTCAGACTACATTGAGCGTCATCTTATATCTTTGTCCATCTGGGAGGAATTACGCTTTTGGGAAGGATACTTTGACTATCTAATGGAACGGTCTTCAAACAAGTCGGCTAACTATGCCTCTTTGGTGGCTGCGCACTTAATTTTTGTGGAATCGCACATGGCTGGGTTGGGAATTCCTGATACTGATGCTTGGTACATGATTGAAACGATAGCAGAGAAGAACAATATTGGATACACACAATTTATAAAGCTGAGAGGGTTCTTGTCGCATATCGAGCACCTACTCATTGGTTATTGGGGAATCACTAATTTCAAGGGTCAATCTATGTTGTCTCATGGATTAGCATCTCCACGTTCAAAAGATGCTACAGTTGAGAATCAGCAGCCTGCTGAAGCTTCTGGTGTTGGAAGGAGCTGGGTTCAGAGCATGTTCAGCAGGGATACTGCATCCAGAACCACCTCTTTAAGTCGTGTTCGTAAATGGACTTCTGATGGTGGCTCTTCAGCTGCAAATGAACATGGAACTCCTCGTAAGCAAGATTTGTCAGCCACTGGCCAGAAGAAAACTCAAACCAATGTTCGCTTACTTAGAGGTCATAGTGGTTCCGTCACTGCTTTACATTGTGTAACGAAGAGAGAGGTTTGGGATCTTGTGGGTGACCGGGAAGATGCCGGTTTCTTCATCAGTGGAAGCACAGACTCCACTGTTAAGATCTGGGATCCCAGTTTTCGTGGTAATGAACTTCGGGCAACCTTGAAAGGACATACAAGAGCTGTCCGTGCAATAAGTTCTGATCGAGGAAAAGTAGTATCAGGTTCGGATCGATCAAACTGTTTTAGTATGGGATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0000278 GO:0000281 GO:0000910 GO:0000911 GO:0002682 GO:0002683 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005886 GO:0007049 GO:0007275 GO:0008150 GO:0009791 GO:0009825 GO:0009888 GO:0009987 GO:0010235 GO:0010374 GO:0010440 GO:0016020 GO:0016049 GO:0022402 GO:0030135 GO:0030136 GO:0031347 GO:0031348 GO:0031410 GO:0031461 GO:0031982 GO:0032501 GO:0032502 GO:0032506 GO:0032991 GO:0040007 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044444 GO:0044464 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0048856 GO:0048869 GO:0050776 GO:0050777 GO:0050789 GO:0051301 GO:0061640 GO:0065007 GO:0071944 GO:0080008 GO:0080134 GO:0090558 GO:0097708 GO:1902410 GO:1902494 GO:1903047 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DENN WD40 dDENN uDENN

Protein Analysis

940

Amino Acids

104.2

Weight (kDa)

5.59

Isoelectric Point (pI)

43.69

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
uDENN	PF03456	35 - 103	1.9e-11	uDENN domain
DENN	PF02141	123 - 304	2.4e-43	DENN (AEX-3) domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0007797)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G49040 AT1G49040 AT1G49040 AT1G49040
fragaria_vesca	FvH4_5g12450 FvH4_5g12450
malus_domestica	MD14G1224200.v1.1
prunus_persica	Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1 Prupe.5G219600_v2.0.a1
pyrus_communis	pycom06g19080 pycom14g18580
rosa_chinensis	RchiOBHm_Chr7g0185021
rosa_laevigata	RLG00000004938
rosa_multiflora	Rmu_ssc0000289.1_g000004
rosa_roxburghii	Rroxscaffold_3G00269260
rosa_rugosa	Rorug06G0466200
rosa_samantha	Rh7AG076700 Rh7BG070700 Rh7CG072200 Rh7DG071200
rosa_wichuraiana	Rw7G005890

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1415, 2216
Acc16I	TGCGCA	1 cut(s)	2103
Acc36I	ACCTGC	2 cut(s)	108, 361
Acc65I	GGTACC	1 cut(s)	1454
AccB1I	GGYRCC	2 cut(s)	65, 1454
AccB7I	CCANNNNNTGG	1 cut(s)	593
AccI	GTMKAC	4 cut(s)	349, 565, 813, 872
AciI	CCGC	8 cut(s)	33, 128, 158, 672, 769, 1156, 1622, 1938
AcoI	YGGCCR	3 cut(s)	879, 1231, 2524
AcsI	RAATTY	7 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142
AcuI	CTGAAG	5 cut(s)	357, 1518, 1857, 2382, 2457
AcyI	GRCGYC	2 cut(s)	66, 228
AdeI	CACNNNGTG	1 cut(s)	25
AfaI	GTAC	5 cut(s)	122, 513, 633, 1456, 2165
AfiI	CCNNNNNNNGG	3 cut(s)	51, 527, 593
AflII	CTTAAG	1 cut(s)	416
AflIII	ACRYGT	1 cut(s)	1944
AjnI	CCWGG	3 cut(s)	526, 1527, 1570
AleI	CACNNNNGTG	2 cut(s)	176, 674
Alw21I	GWGCWC	2 cut(s)	21, 2250
Alw26I	GTCTC	2 cut(s)	854, 2307
AlwNI	CAGNNNCTG	4 cut(s)	1264, 1528, 1669, 2522
Ama87I	CYCGRG	1 cut(s)	44
AoxI	GGCC	4 cut(s)	879, 1231, 1363, 2524
ApeKI	GCWGC	8 cut(s)	775, 1532, 1535, 1619, 1660, 2099, 2353, 2477
ApoI	RAATTY	7 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142
ArsI	GACNNNNNNTTYG	2 cut(s)	33, 65
Asp718I	GGTACC	1 cut(s)	1454
AspLEI	GCGC	4 cut(s)	68, 829, 1218, 2104
AspS9I	GGNCC	5 cut(s)	41, 54, 115, 1363, 1694
AsuC2I	CCSGG	2 cut(s)	311, 2633
AsuHPI	GGTGA	4 cut(s)	284, 390, 661, 2639
AvaI	CYCGRG	1 cut(s)	44
AvaII	GGWCC	4 cut(s)	41, 54, 115, 1694
AxyI	CCTNAGG	1 cut(s)	1490
BaeI	ACNNNNGTAYC	4 cut(s)	717, 750, 1419, 1452
BalI	TGGCCA	3 cut(s)	881, 1233, 2526
BamHI	GGATCC	2 cut(s)	313, 2689
BanI	GGYRCC	2 cut(s)	65, 1454
BanII	GRGCYC	1 cut(s)	600
BarI	GAAGNNNNNNTAC	2 cut(s)	2440, 2472
BbsI	GAAGAC	3 cut(s)	1180, 1673, 2055
Bbv12I	GWGCWC	2 cut(s)	21, 2250
BbvCI	CCTCAGC	2 cut(s)	162, 1761
BbvI	GCAGC	8 cut(s)	787, 1519, 1547, 1606, 1647, 2086, 2365, 2464
BccI	CCATC	8 cut(s)	269, 587, 838, 881, 1028, 1812, 2015, 2456
BceAI	ACGGC	2 cut(s)	79, 88
BcgI	CGANNNNNNTGC	2 cut(s)	1720, 1754
BciT130I	CCWGG	3 cut(s)	528, 1529, 1572
BciVI	GTATCC	6 cut(s)	745, 1011, 1945, 2030, 2195, 2404
BcnI	CCSGG	2 cut(s)	311, 2633
BcoDI	GTCTC	2 cut(s)	854, 2307
BfaI	CTAG	3 cut(s)	641, 1292, 1554
BfmI	CTRYAG	3 cut(s)	1533, 1661, 2337
BfoI	RGCGCY	1 cut(s)	69
BfrI	CTTAAG	1 cut(s)	416
BfuAI	ACCTGC	2 cut(s)	108, 361
BfuI	GTATCC	6 cut(s)	745, 1011, 1945, 2030, 2195, 2404
BglII	AGATCT	1 cut(s)	2682
BisI	GCNGC	8 cut(s)	776, 1533, 1536, 1620, 1661, 2100, 2354, 2478
BlsI	GCNGC	8 cut(s)	777, 1534, 1537, 1621, 1662, 2101, 2355, 2479
Bme1390I	CCNGG	5 cut(s)	311, 528, 1529, 1572, 2633
Bme18I	GGWCC	4 cut(s)	41, 54, 115, 1694
BmeT110I	CYCGRG	1 cut(s)	44
BmgT120I	GGNCC	5 cut(s)	41, 54, 115, 1363, 1694
BmrFI	CCNGG	5 cut(s)	311, 528, 1529, 1572, 2633
BmrI	ACTGGG	1 cut(s)	2688
BmuI	ACTGGG	1 cut(s)	2688
BoxI	GACNNNNGTC	1 cut(s)	561
BpiI	GAAGAC	3 cut(s)	1180, 1673, 2055
BpmI	CTGGAG	2 cut(s)	449, 510
Bpu10I	CCTNAGC	2 cut(s)	162, 1761
BpuEI	CTTGAG	2 cut(s)	929, 1182
BpuMI	CCSGG	2 cut(s)	311, 2633
Bsa29I	ATCGAT	1 cut(s)	2794
BsaBI	GATNNNNATC	1 cut(s)	2314
BsaHI	GRCGYC	2 cut(s)	66, 228
BsaI	GGTCTC	1 cut(s)	854
BsaJI	CCNNGG	5 cut(s)	79, 242, 882, 1082, 1571
BsaXI	ACNNNNNCTCC	2 cut(s)	1588, 1618
Bsc4I	CCNNNNNNNGG	3 cut(s)	51, 527, 593
Bse118I	RCCGGY	2 cut(s)	188, 2642
Bse1I	ACTGG	6 cut(s)	118, 317, 432, 1234, 2527, 2694
Bse21I	CCTNAGG	1 cut(s)	1490
Bse3DI	GCAATG	3 cut(s)	300, 764, 1218
Bse8I	GATNNNNATC	1 cut(s)	2314
BseBI	CCWGG	3 cut(s)	528, 1529, 1572
BseCI	ATCGAT	1 cut(s)	2794
BseDI	CCNNGG	5 cut(s)	79, 242, 882, 1082, 1571
BseGI	GGATG	8 cut(s)	598, 892, 1039, 1438, 1823, 1927, 1963, 2417
BseJI	GATNNNNATC	1 cut(s)	2314
BseLI	CCNNNNNNNGG	3 cut(s)	51, 527, 593
BseMI	GCAATG	3 cut(s)	300, 764, 1218
BseMII	CTCAG	7 cut(s)	176, 258, 1044, 1398, 1497, 1752, 2213
BseNI	ACTGG	6 cut(s)	118, 317, 432, 1234, 2527, 2694
BseRI	GAGGAG	3 cut(s)	141, 144, 2487
BseXI	GCAGC	8 cut(s)	787, 1519, 1547, 1606, 1647, 2086, 2365, 2464
BseYI	CCCAGC	3 cut(s)	401, 2132, 2385
BshFI	GGCC	4 cut(s)	881, 1233, 1365, 2526
BshNI	GGYRCC	2 cut(s)	65, 1454
BshVI	ATCGAT	1 cut(s)	2794
BsiHKAI	GWGCWC	2 cut(s)	21, 2250
BsiHKCI	CYCGRG	1 cut(s)	44
BsiSI	CCGG	4 cut(s)	189, 310, 2632, 2643
BslFI	GGGAC	2 cut(s)	292, 1707
BslI	CCNNNNNNNGG	3 cut(s)	51, 527, 593
BsmAI	GTCTC	2 cut(s)	854, 2307
BsmFI	GGGAC	2 cut(s)	292, 1707
BsmI	GAATGC	2 cut(s)	427, 1202
BsnI	GGCC	4 cut(s)	881, 1233, 1365, 2526
Bso31I	GGTCTC	1 cut(s)	854
BsoBI	CYCGRG	1 cut(s)	44
Bsp1286I	GDGCHC	3 cut(s)	21, 600, 2250
Bsp19I	CCATGG	1 cut(s)	882
BspACI	CCGC	8 cut(s)	33, 128, 158, 672, 769, 1156, 1622, 1938
BspANI	GGCC	4 cut(s)	881, 1233, 1365, 2526
BspCNI	CTCAG	7 cut(s)	175, 259, 1045, 1399, 1496, 1753, 2214
BspDI	ATCGAT	1 cut(s)	2794
BspMAI	CTGCAG	2 cut(s)	1537, 1665
BspMI	ACCTGC	2 cut(s)	108, 361
BspQI	GCTCTTC	2 cut(s)	1494, 2476
BspT107I	GGYRCC	2 cut(s)	65, 1454
BspTI	CTTAAG	1 cut(s)	416
BspTNI	GGTCTC	1 cut(s)	854
BsrDI	GCAATG	3 cut(s)	300, 764, 1218
BsrFI	RCCGGY	2 cut(s)	188, 2642
BsrI	ACTGG	6 cut(s)	118, 317, 432, 1234, 2527, 2694
BssAI	RCCGGY	2 cut(s)	188, 2642
BssECI	CCNNGG	5 cut(s)	79, 242, 882, 1082, 1571
BssNAI	GTATAC	1 cut(s)	350
BssNI	GRCGYC	2 cut(s)	66, 228
BssT1I	CCWWGG	2 cut(s)	882, 1082
Bst1107I	GTATAC	1 cut(s)	350
Bst2UI	CCWGG	3 cut(s)	528, 1529, 1572
Bst6I	CTCTTC	3 cut(s)	1494, 2476, 2597
BstACI	GRCGYC	2 cut(s)	66, 228
BstAFI	CTTAAG	1 cut(s)	416
BstC8I	GCNNGC	3 cut(s)	186, 1072, 2358
BstDSI	CCRYGG	3 cut(s)	79, 242, 882
BstEII	GGTNACC	2 cut(s)	396, 2627
BstENI	CCTNNNNNAGG	1 cut(s)	525
BstF5I	GGATG	8 cut(s)	598, 892, 1039, 1438, 1823, 1927, 1963, 2417
BstH2I	RGCGCY	1 cut(s)	69
BstHHI	GCGC	4 cut(s)	68, 829, 1218, 2104
BstMAI	GTCTC	2 cut(s)	854, 2307
BstMWI	GCNNNNNNNGC	6 cut(s)	775, 1208, 2084, 2245, 2403, 2750
BstNI	CCWGG	3 cut(s)	528, 1529, 1572
BstNSI	RCATGY	2 cut(s)	1948, 2401
BstPAI	GACNNNNGTC	1 cut(s)	561
BstPI	GGTNACC	2 cut(s)	396, 2627
BstSCI	CCNGG	5 cut(s)	309, 526, 1527, 1570, 2631
BstSFI	CTRYAG	3 cut(s)	1533, 1661, 2337
BstV1I	GCAGC	8 cut(s)	787, 1519, 1547, 1606, 1647, 2086, 2365, 2464
BstV2I	GAAGAC	3 cut(s)	1180, 1673, 2055
BstX2I	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
BstXI	CCANNNNNNTGG	2 cut(s)	771, 1012
BstYI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
BstZ17I	GTATAC	1 cut(s)	350
Bsu15I	ATCGAT	1 cut(s)	2794
Bsu36I	CCTNAGG	1 cut(s)	1490
BsuI	GTATCC	6 cut(s)	745, 1011, 1945, 2030, 2195, 2404
BsuRI	GGCC	4 cut(s)	881, 1233, 1365, 2526
BsuTUI	ATCGAT	1 cut(s)	2794
BtgI	CCRYGG	3 cut(s)	79, 242, 882
BtsCI	GGATG	8 cut(s)	598, 892, 1039, 1438, 1823, 1927, 1963, 2417
BtsI	GCAGTG	2 cut(s)	1783, 2580
BveI	ACCTGC	2 cut(s)	108, 361
Cac8I	GCNNGC	3 cut(s)	186, 1072, 2358
CaiI	CAGNNNCTG	4 cut(s)	1264, 1528, 1669, 2522
CfoI	GCGC	4 cut(s)	68, 829, 1218, 2104
Cfr10I	RCCGGY	2 cut(s)	188, 2642
Cfr13I	GGNCC	5 cut(s)	41, 54, 115, 1363, 1694
ClaI	ATCGAT	1 cut(s)	2794
CseI	GACGC	2 cut(s)	236, 1976
Csp6I	GTAC	5 cut(s)	121, 512, 632, 1455, 2164
CspCI	CAANNNNNGTGG	2 cut(s)	958, 993
CviQI	GTAC	5 cut(s)	121, 512, 632, 1455, 2164
DinI	GGCGCC	1 cut(s)	67
DraIII	CACNNNGTG	1 cut(s)	25
EaeI	YGGCCR	3 cut(s)	879, 1231, 2524
Eam1104I	CTCTTC	3 cut(s)	1494, 2476, 2597
EarI	CTCTTC	3 cut(s)	1494, 2476, 2597
EciI	GGCGGA	2 cut(s)	117, 147
Eco130I	CCWWGG	2 cut(s)	882, 1082
Eco24I	GRGCYC	1 cut(s)	600
Eco31I	GGTCTC	1 cut(s)	854
Eco32I	GATATC	1 cut(s)	987
Eco47I	GGWCC	4 cut(s)	41, 54, 115, 1694
Eco57I	CTGAAG	5 cut(s)	357, 1518, 1857, 2382, 2457
Eco81I	CCTNAGG	1 cut(s)	1490
Eco88I	CYCGRG	1 cut(s)	44
Eco91I	GGTNACC	2 cut(s)	396, 2627
EcoNI	CCTNNNNNAGG	1 cut(s)	525
EcoO109I	RGGNCCY	1 cut(s)	1694
EcoO65I	GGTNACC	2 cut(s)	396, 2627
EcoRI	GAATTC	1 cut(s)	2142
EcoRII	CCWGG	3 cut(s)	526, 1527, 1570
EcoRV	GATATC	1 cut(s)	987
EcoT14I	CCWWGG	2 cut(s)	882, 1082
EcoT38I	GRGCYC	1 cut(s)	600
EgeI	GGCGCC	1 cut(s)	67
EheI	GGCGCC	1 cut(s)	67
ErhI	CCWWGG	2 cut(s)	882, 1082
FalI	AAGNNNNNCTT	2 cut(s)	1338, 1370
FaqI	GGGAC	2 cut(s)	292, 1707
FauI	CCCGC	1 cut(s)	26
FauNDI	CATATG	1 cut(s)	1468
FblI	GTMKAC	4 cut(s)	349, 565, 813, 872
Fnu4HI	GCNGC	8 cut(s)	776, 1533, 1536, 1620, 1661, 2100, 2354, 2478
FokI	GGATG	8 cut(s)	605, 899, 1046, 1445, 1830, 1934, 1970, 2404
FriOI	GRGCYC	1 cut(s)	600
Fsp4HI	GCNGC	8 cut(s)	776, 1533, 1536, 1620, 1661, 2100, 2354, 2478
FspBI	CTAG	3 cut(s)	641, 1292, 1554
FspI	TGCGCA	1 cut(s)	2103
GlaI	GCGC	4 cut(s)	67, 828, 1217, 2103
GluI	GCNGC	8 cut(s)	776, 1533, 1536, 1620, 1661, 2100, 2354, 2478
GsaI	CCCAGC	3 cut(s)	405, 2136, 2389
GsuI	CTGGAG	2 cut(s)	449, 510
HaeII	RGCGCY	1 cut(s)	69
HaeIII	GGCC	4 cut(s)	881, 1233, 1365, 2526
HapII	CCGG	4 cut(s)	189, 310, 2632, 2643
HgaI	GACGC	2 cut(s)	236, 1976
HhaI	GCGC	4 cut(s)	68, 829, 1218, 2104
Hin1I	GRCGYC	2 cut(s)	66, 228
Hin6I	GCGC	4 cut(s)	66, 827, 1216, 2102
HinP1I	GCGC	4 cut(s)	66, 827, 1216, 2102
HincII	GTYRAC	5 cut(s)	649, 904, 1227, 1833, 1911
HindII	GTYRAC	5 cut(s)	649, 904, 1227, 1833, 1911
HindIII	AAGCTT	2 cut(s)	339, 2364
HpaII	CCGG	4 cut(s)	189, 310, 2632, 2643
HphI	GGTGA	4 cut(s)	284, 390, 661, 2639
Hpy188III	TCNNGA	8 cut(s)	113, 1097, 1265, 1292, 1446, 1896, 2147, 2421
Hpy99I	CGWCG	3 cut(s)	65, 109, 211
HpyAV	CCTTC	6 cut(s)	1112, 1353, 1832, 1948, 2027, 2373
HpyCH4IV	ACGT	3 cut(s)	236, 869, 2323
HpyF10VI	GCNNNNNNNGC	6 cut(s)	775, 1208, 2084, 2245, 2403, 2750
HpySE526I	ACGT	3 cut(s)	236, 869, 2323
Hsp92I	GRCGYC	2 cut(s)	66, 228
HspAI	GCGC	4 cut(s)	66, 827, 1216, 2102
KasI	GGCGCC	1 cut(s)	65
KpnI	GGTACC	1 cut(s)	1458
LguI	GCTCTTC	2 cut(s)	1494, 2476
LmnI	GCTCC	5 cut(s)	171, 246, 586, 603, 2382
Lsp1109I	GCAGC	8 cut(s)	787, 1519, 1547, 1606, 1647, 2086, 2365, 2464
MaeI	CTAG	3 cut(s)	641, 1292, 1554
MaeII	ACGT	3 cut(s)	236, 869, 2323
MaeIII	GTNAC	7 cut(s)	396, 792, 1094, 1341, 2578, 2596, 2627
MfeI	CAATTG	2 cut(s)	559, 1419
MflI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
MhlI	GDGCHC	3 cut(s)	21, 600, 2250
MlsI	TGGCCA	3 cut(s)	881, 1233, 2526
MluNI	TGGCCA	3 cut(s)	881, 1233, 2526
Mly113I	GGCGCC	1 cut(s)	66
MlyI	GAGTC	2 cut(s)	1577, 2663
MmeI	TCCRAC	1 cut(s)	2356
Mox20I	TGGCCA	3 cut(s)	881, 1233, 2526
MscI	TGGCCA	3 cut(s)	881, 1233, 2526
MslI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1253
Msp20I	TGGCCA	3 cut(s)	881, 1233, 2526
MspA1I	CMGCKG	3 cut(s)	674, 1940, 2477
MspCI	CTTAAG	1 cut(s)	416
MspI	CCGG	4 cut(s)	189, 310, 2632, 2643
MspR9I	CCNGG	5 cut(s)	311, 528, 1529, 1572, 2633
MunI	CAATTG	2 cut(s)	559, 1419
Mva1269I	GAATGC	2 cut(s)	427, 1202
MvaI	CCWGG	3 cut(s)	528, 1529, 1572
MwoI	GCNNNNNNNGC	6 cut(s)	775, 1208, 2084, 2245, 2403, 2750
NarI	GGCGCC	1 cut(s)	66
NciI	CCSGG	2 cut(s)	311, 2633
NcoI	CCATGG	1 cut(s)	882
NdeI	CATATG	1 cut(s)	1468
NmuCI	GTSAC	4 cut(s)	396, 1094, 2578, 2627
NsbI	TGCGCA	1 cut(s)	2103
NspI	RCATGY	2 cut(s)	1948, 2401
OliI	CACNNNNGTG	2 cut(s)	176, 674
PciI	ACATGT	1 cut(s)	1944
PciSI	GCTCTTC	2 cut(s)	1494, 2476
PcsI	WCGNNNNNNNCGW	1 cut(s)	21
PctI	GAATGC	2 cut(s)	427, 1202
PfeI	GAWTC	8 cut(s)	49, 75, 218, 1268, 1751, 2120, 2271, 2347
PflMI	CCANNNNNTGG	1 cut(s)	593
PfoI	TCCNGGA	2 cut(s)	309, 526
PkrI	GCNGC	8 cut(s)	777, 1534, 1537, 1621, 1662, 2101, 2355, 2479
PleI	GAGTC	2 cut(s)	1577, 2663
PluTI	GGCGCC	1 cut(s)	69
PpsI	GAGTC	2 cut(s)	1577, 2663
PpuMI	RGGWCCY	1 cut(s)	1694
PscI	ACATGT	1 cut(s)	1944
PshAI	GACNNNNGTC	1 cut(s)	561
PsiI	TTATAA	2 cut(s)	1415, 2216
Psp5II	RGGWCCY	1 cut(s)	1694
Psp6I	CCWGG	3 cut(s)	526, 1527, 1570
PspEI	GGTNACC	2 cut(s)	396, 2627
PspFI	CCCAGC	3 cut(s)	401, 2132, 2385
PspGI	CCWGG	3 cut(s)	526, 1527, 1570
PspPI	GGNCC	5 cut(s)	41, 54, 115, 1363, 1694
PspPPI	RGGWCCY	1 cut(s)	1694
PstI	CTGCAG	2 cut(s)	1537, 1665
PstNI	CAGNNNCTG	4 cut(s)	1264, 1528, 1669, 2522
PsuI	RGATCY	6 cut(s)	9, 313, 1524, 2617, 2682, 2689
PvuII	CAGCTG	1 cut(s)	2477
RsaI	GTAC	5 cut(s)	122, 513, 633, 1456, 2165
RsaNI	GTAC	5 cut(s)	121, 512, 632, 1455, 2164
RseI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1253
SapI	GCTCTTC	2 cut(s)	1494, 2476
SatI	GCNGC	8 cut(s)	776, 1533, 1536, 1620, 1661, 2100, 2354, 2478
Sau96I	GGNCC	5 cut(s)	41, 54, 115, 1363, 1694
SchI	GAGTC	2 cut(s)	1577, 2663
ScrFI	CCNGG	5 cut(s)	311, 528, 1529, 1572, 2633
SduI	GDGCHC	3 cut(s)	21, 600, 2250
SfcI	CTRYAG	3 cut(s)	1533, 1661, 2337
SfoI	GGCGCC	1 cut(s)	67
SinI	GGWCC	4 cut(s)	41, 54, 115, 1694
SmiMI	CAYNNNNRTG	5 cut(s)	176, 674, 769, 858, 1253
SmlI	CTYRAG	3 cut(s)	416, 908, 1197
SmoI	CTYRAG	3 cut(s)	416, 908, 1197
SsiI	CCGC	8 cut(s)	33, 128, 158, 672, 769, 1156, 1622, 1938
SspDI	GGCGCC	1 cut(s)	65
SspI	AATATT	3 cut(s)	716, 1061, 2197
SspMI	CTAG	3 cut(s)	641, 1292, 1554
StyD4I	CCNGG	5 cut(s)	309, 526, 1527, 1570, 2631
StyI	CCWWGG	2 cut(s)	882, 1082
TaiI	ACGT	3 cut(s)	239, 872, 2326
TaqI	TCGA	6 cut(s)	15, 60, 87, 2244, 2767, 2794
TaqII	GACCGA	2 cut(s)	552, 1212
TfiI	GAWTC	8 cut(s)	49, 75, 218, 1268, 1751, 2120, 2271, 2347
TseFI	GTSAC	4 cut(s)	396, 1094, 2578, 2627
TseI	GCWGC	8 cut(s)	775, 1532, 1535, 1619, 1660, 2099, 2353, 2477
Tsp45I	GTSAC	4 cut(s)	396, 1094, 2578, 2627
TspDTI	ATGAA	8 cut(s)	351, 1038, 1153, 1270, 1707, 2499, 2641, 2724
TspGWI	ACGGA	6 cut(s)	259, 780, 947, 1445, 2566, 2738
Van91I	CCANNNNNTGG	1 cut(s)	593
Vha464I	CTTAAG	1 cut(s)	416
VpaK11BI	GGWCC	4 cut(s)	41, 54, 115, 1694
XagI	CCTNNNNNAGG	1 cut(s)	525
XapI	RAATTY	7 cut(s)	285, 358, 662, 1170, 1393, 1441, 2142
XbaI	TCTAGA	1 cut(s)	1291
XceI	RCATGY	2 cut(s)	1948, 2401
XmiI	GTMKAC	4 cut(s)	349, 565, 813, 872
XspI	CTAG	3 cut(s)	641, 1292, 1554

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.