pycom14g18820

Protein of unknown function (DUF1666)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0008073
Physical Location & Seq
Reverse (-)
20387015 .. 20389129
2115 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1374 bp
ATGATCGATGATCTGGTGGCTGACATCATGCGCGGCAACGAATCACTAAGGTTTTTGCCCAACAATAACCTTCAGCATCATCATCAAAAAAAAAATTCCGAAGACCAGTTCATTAGCGCACGGGAGAGCTTAATAATTGAAGAGCGCCAAGATGAATCTTTGCATGAGTCACCACAAGGTTCAGAATCTGAATATGACGATCAAGGTGTGGAGGAAATCCCAGTGAAAGAAACAGCGTCAGTTCTCAATTCTGTCGCGAATGATCGAGTGTGGTCCACATCTCCGATAACTATAGAACCACATAAAAGTGACAAAAATGGTGACGATAATTGTGATGAACACAACACTGAAATCAATAGGGCAGATCAGACGCATTTATCCGGTGACGAAAATTTTGTTGTTTATGGGCCATCACAATTGAAGAACAAGAAGTTCCAAGTTCAAAACAATAAAGAGGATGAAATTTTTGGTGACTCAACCGTTGGATCAACTTCTAAGAGCTCTTCTGAATGGAGAAGCTCAATTAACTGCAGGGATTCTGCCACTGAAGATCCTTTTTCCTCCTCGTCCCGGAGAAGCTGCCCCAAATGGGAGTCTTACACAGTCTTCCAAAAGTATGATGAAGAAATGATGTTCTTAGACAGAATTAGTGCCCAAAAGCTCCATGAAACAGAATCACTTAAGTCTATTCAAGTTTGTCCAAGATCGATTTCGGAAAGGATAGTTCATAGAATCTCTATGGCAACAAAAAGGCCGCCTGATGTACGTCACAACCCTTATCATGAGCTGGAGGCTGCTTACGTTGCACAAATTTGCTTAACATGGGAAGCACTTAATTGGAACTACAAGAATTTTGAGCAAAAAAGGGCATCACGACGTGATCAGTTTGATCCCGGCGTTCCTGGCGAATTAGCACAGCAATTTCAGCAATTTCAAGTGCTTCTACGAAGATATGTAGAGAATGAGCCTTATGAGCAAGGCAGGAGGCCAGAGATTTATGCTAGGATGAGGCTTTTGGCACCAAAGTTACTTCAAGTGCCAGAATATCAAGTTTCAGAAGATGGTGAGCATGAAAAGGACGAAGGTTTTGGCTCAAAAATTTCATCAGCTGCATTTTTAATGATAATGGAAGACGGAATCCGAACGTTCATGGATTTTCTCAAGGCTGATAAGATAAAACCCTGCCAGAAATTAGCATCCATGTTCAAAAGAAAACGAAAAGGTTCAGTTGATCCCACTCTTCTCCATCTGATGAAGAAAGTAAATCAAAAAAAGAAGATGAAAATCAAAGATCGTTCGACGTTCCCACAAATGCATGAGAAAGCGAAGCTTGAAGGTGGAGGAAGAGATGGGGATACTGATGGGTCTAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1666

Protein Analysis

458

Amino Acids

52.7

Weight (kDa)

5.86

Isoelectric Point (pI)

53.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1666 PF07891 261 - 433 1.2e-58 Protein of unknown function (DUF1666)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1018
AccII CGCG 2 cut(s) 33, 257
AciI CCGC 2 cut(s) 33, 755
AclI AACGTT 1 cut(s) 1145
AclWI GGATC 4 cut(s) 493, 545, 884, 1226
AcsI RAATTY 6 cut(s) 94, 391, 462, 810, 850, 1098
AcuI CTGAAG 2 cut(s) 56, 567
AdeI CACNNNGTG 1 cut(s) 878
AfaI GTAC 1 cut(s) 765
AfiI CCNNNNNNNGG 2 cut(s) 570, 589
AflII CTTAAG 1 cut(s) 680
AgsI TTSAA 8 cut(s) 140, 421, 443, 692, 935, 1034, 1207, 1334
AjiI CACGTC 1 cut(s) 878
AjnI CCWGG 1 cut(s) 901
AjuI GAANNNNNNNTTGG 2 cut(s) 694, 726
AluBI AGCT 8 cut(s) 129, 501, 519, 579, 661, 787, 1109, 1330
AluI AGCT 8 cut(s) 129, 501, 519, 579, 661, 787, 1109, 1330
Alw21I GWGCWC 1 cut(s) 503
AlwI GGATC 4 cut(s) 493, 545, 884, 1226
AlwNI CAGNNNCTG 1 cut(s) 188
AoxI GGCC 3 cut(s) 407, 752, 986
ApeKI GCWGC 3 cut(s) 579, 794, 1109
ApoI RAATTY 6 cut(s) 94, 391, 462, 810, 850, 1098
ArsI GACNNNNNNTTYG 4 cut(s) 377, 409, 1070, 1102
Asp700I GAANNNNTTC 1 cut(s) 1222
AspLEI GCGC 3 cut(s) 33, 119, 147
AspS9I GGNCC 2 cut(s) 273, 407
AsuC2I CCSGG 2 cut(s) 571, 894
AsuHPI GGTGA 5 cut(s) 162, 332, 395, 482, 1076
AvaII GGWCC 1 cut(s) 273
BaeGI GKGCMC 1 cut(s) 655
BanI GGYRCC 1 cut(s) 1018
BanII GRGCYC 1 cut(s) 503
BbsI GAAGAC 3 cut(s) 108, 598, 1137
Bbv12I GWGCWC 1 cut(s) 503
BbvI GCAGC 3 cut(s) 566, 781, 1096
BccI CCATC 5 cut(s) 418, 1055, 1254, 1343, 1355
BciT130I CCWGG 1 cut(s) 903
BciVI GTATCC 1 cut(s) 1348
BclI TGATCA 1 cut(s) 880
BcnI CCSGG 2 cut(s) 571, 894
BfaI CTAG 1 cut(s) 1002
BfmI CTRYAG 2 cut(s) 291, 529
BfoI RGCGCY 1 cut(s) 148
BfrI CTTAAG 1 cut(s) 680
BfuI GTATCC 1 cut(s) 1348
BisI GCNGC 5 cut(s) 34, 580, 755, 795, 1110
BlsI GCNGC 5 cut(s) 35, 581, 756, 796, 1111
Bme1390I CCNGG 3 cut(s) 571, 894, 903
Bme18I GGWCC 1 cut(s) 273
BmgBI CACGTC 1 cut(s) 878
BmgT120I GGNCC 2 cut(s) 273, 407
BmiI GGNNCC 1 cut(s) 1020
BmrFI CCNGG 3 cut(s) 571, 894, 903
BmrI ACTGGG 1 cut(s) 215
BmsI GCATC 3 cut(s) 85, 878, 1205
BmuI ACTGGG 1 cut(s) 215
BpiI GAAGAC 3 cut(s) 108, 598, 1137
BpmI CTGGAG 1 cut(s) 809
BpuEI CTTGAG 1 cut(s) 1145
BpuMI CCSGG 2 cut(s) 571, 894
Bsa29I ATCGAT 2 cut(s) 6, 707
BsaBI GATNNNNATC 1 cut(s) 1283
BsaWI WCCGGW 1 cut(s) 380
Bsc4I CCNNNNNNNGG 2 cut(s) 570, 589
Bse1I ACTGG 2 cut(s) 106, 221
Bse8I GATNNNNATC 1 cut(s) 1283
BseBI CCWGG 1 cut(s) 903
BseCI ATCGAT 2 cut(s) 6, 707
BseGI GGATG 3 cut(s) 463, 1011, 1196
BseJI GATNNNNATC 1 cut(s) 1283
BseLI CCNNNNNNNGG 2 cut(s) 570, 589
BseNI ACTGG 2 cut(s) 106, 221
BseRI GAGGAG 1 cut(s) 553
BseSI GKGCMC 1 cut(s) 655
BseXI GCAGC 3 cut(s) 566, 781, 1096
Bsh1236I CGCG 2 cut(s) 33, 257
BshFI GGCC 3 cut(s) 409, 754, 988
BshNI GGYRCC 1 cut(s) 1018
BshVI ATCGAT 2 cut(s) 6, 707
BsiHKAI GWGCWC 1 cut(s) 503
BsiSI CCGG 3 cut(s) 381, 571, 894
BslFI GGGAC 1 cut(s) 553
BslI CCNNNNNNNGG 2 cut(s) 570, 589
BsmFI GGGAC 1 cut(s) 553
BsnI GGCC 3 cut(s) 409, 754, 988
Bsp1286I GDGCHC 2 cut(s) 503, 655
Bsp68I TCGCGA 1 cut(s) 257
BspACI CCGC 2 cut(s) 33, 755
BspANI GGCC 3 cut(s) 409, 754, 988
BspDI ATCGAT 2 cut(s) 6, 707
BspFNI CGCG 2 cut(s) 33, 257
BspHI TCATGA 1 cut(s) 781
BspLI GGNNCC 1 cut(s) 1020
BspMAI CTGCAG 1 cut(s) 533
BspPI GGATC 4 cut(s) 493, 545, 884, 1226
BspQI GCTCTTC 2 cut(s) 135, 508
BspT107I GGYRCC 1 cut(s) 1018
BspTI CTTAAG 1 cut(s) 680
BsrI ACTGG 2 cut(s) 106, 221
Bst2UI CCWGG 1 cut(s) 903
Bst4CI ACNGT 2 cut(s) 481, 604
Bst6I CTCTTC 4 cut(s) 135, 508, 1245, 1339
BstAFI CTTAAG 1 cut(s) 680
BstDEI CTNAG 3 cut(s) 47, 495, 637
BstF5I GGATG 3 cut(s) 463, 1011, 1196
BstFNI CGCG 2 cut(s) 33, 257
BstH2I RGCGCY 1 cut(s) 148
BstHHI GCGC 3 cut(s) 33, 119, 147
BstMWI GCNNNNNNNGC 4 cut(s) 803, 903, 925, 973
BstNI CCWGG 1 cut(s) 903
BstSCI CCNGG 3 cut(s) 569, 892, 901
BstSFI CTRYAG 2 cut(s) 291, 529
BstSLI GKGCMC 1 cut(s) 655
BstUI CGCG 2 cut(s) 33, 257
BstV1I GCAGC 3 cut(s) 566, 781, 1096
BstV2I GAAGAC 3 cut(s) 108, 598, 1137
BstX2I RGATCY 1 cut(s) 550
BstYI RGATCY 1 cut(s) 550
Bsu15I ATCGAT 2 cut(s) 6, 707
BsuI GTATCC 1 cut(s) 1348
BsuRI GGCC 3 cut(s) 409, 754, 988
BsuTUI ATCGAT 2 cut(s) 6, 707
BtrI CACGTC 1 cut(s) 878
BtsCI GGATG 3 cut(s) 463, 1011, 1196
BtsIMutI CAGTG 3 cut(s) 228, 345, 543
BtuMI TCGCGA 1 cut(s) 257
CaiI CAGNNNCTG 1 cut(s) 188
CciI TCATGA 1 cut(s) 781
CfoI GCGC 3 cut(s) 33, 119, 147
Cfr13I GGNCC 2 cut(s) 273, 407
ClaI ATCGAT 2 cut(s) 6, 707
CseI GACGC 2 cut(s) 225, 379
Csp6I GTAC 1 cut(s) 764
CviAII CATG 9 cut(s) 28, 164, 665, 782, 822, 1070, 1150, 1201, 1316
CviQI GTAC 1 cut(s) 764
DdeI CTNAG 3 cut(s) 47, 495, 637
DraIII CACNNNGTG 1 cut(s) 878
Eam1104I CTCTTC 4 cut(s) 135, 508, 1245, 1339
EarI CTCTTC 4 cut(s) 135, 508, 1245, 1339
Ecl136II GAGCTC 1 cut(s) 501
Eco24I GRGCYC 1 cut(s) 503
Eco47I GGWCC 1 cut(s) 273
Eco53kI GAGCTC 1 cut(s) 501
Eco57I CTGAAG 2 cut(s) 56, 567
EcoICRI GAGCTC 1 cut(s) 501
EcoRII CCWGG 1 cut(s) 901
EcoT22I ATGCAT 1 cut(s) 1317
EcoT38I GRGCYC 1 cut(s) 503
FaeI CATG 9 cut(s) 31, 167, 668, 785, 825, 1073, 1153, 1204, 1319
FalI AAGNNNNNCTT 2 cut(s) 1314, 1346
FaqI GGGAC 1 cut(s) 553
FatI CATG 9 cut(s) 27, 163, 664, 781, 821, 1069, 1149, 1200, 1315
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 5 cut(s) 34, 580, 755, 795, 1110
FokI GGATG 3 cut(s) 470, 1018, 1183
FriOI GRGCYC 1 cut(s) 503
Fsp4HI GCNGC 5 cut(s) 34, 580, 755, 795, 1110
FspBI CTAG 1 cut(s) 1002
GlaI GCGC 3 cut(s) 32, 118, 146
GluI GCNGC 5 cut(s) 34, 580, 755, 795, 1110
GsuI CTGGAG 1 cut(s) 809
HaeII RGCGCY 1 cut(s) 148
HaeIII GGCC 3 cut(s) 409, 754, 988
HapII CCGG 3 cut(s) 381, 571, 894
HgaI GACGC 2 cut(s) 225, 379
HhaI GCGC 3 cut(s) 33, 119, 147
Hin1II CATG 9 cut(s) 31, 167, 668, 785, 825, 1073, 1153, 1204, 1319
Hin6I GCGC 3 cut(s) 31, 117, 145
HinP1I GCGC 3 cut(s) 31, 117, 145
HindIII AAGCTT 1 cut(s) 1328
HpaII CCGG 3 cut(s) 381, 571, 894
HphI GGTGA 5 cut(s) 162, 332, 395, 482, 1076
Hpy166II GTNNAC 1 cut(s) 276
Hpy188III TCNNGA 3 cut(s) 256, 782, 873
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 2 cut(s) 879, 1303
HpyAV CCTTC 3 cut(s) 80, 1076, 1328
HpyCH4III ACNGT 2 cut(s) 481, 604
HpyCH4IV ACGT 5 cut(s) 766, 801, 877, 1145, 1301
HpyCH4V TGCA 5 cut(s) 163, 531, 806, 1112, 1315
HpyF10VI GCNNNNNNNGC 4 cut(s) 803, 903, 925, 973
HpyF3I CTNAG 3 cut(s) 47, 495, 637
HpySE526I ACGT 5 cut(s) 766, 801, 877, 1145, 1301
Hsp92II CATG 9 cut(s) 31, 167, 668, 785, 825, 1073, 1153, 1204, 1319
HspAI GCGC 3 cut(s) 31, 117, 145
Ksp22I TGATCA 1 cut(s) 880
LguI GCTCTTC 2 cut(s) 135, 508
LmnI GCTCC 1 cut(s) 666
Lsp1109I GCAGC 3 cut(s) 566, 781, 1096
LweI GCATC 3 cut(s) 85, 878, 1205
MaeI CTAG 1 cut(s) 1002
MaeII ACGT 5 cut(s) 766, 801, 877, 1145, 1301
MaeIII GTNAC 7 cut(s) 168, 308, 320, 383, 470, 767, 1026
MfeI CAATTG 1 cut(s) 416
MflI RGATCY 1 cut(s) 550
MhlI GDGCHC 2 cut(s) 503, 655
MlyI GAGTC 3 cut(s) 176, 467, 602
MmeI TCCRAC 1 cut(s) 463
MnlI CCTC 8 cut(s) 205, 448, 571, 574, 784, 978, 1002, 1334
Mph1103I ATGCAT 1 cut(s) 1317
MroXI GAANNNNTTC 1 cut(s) 1222
MseI TTAA 6 cut(s) 131, 525, 681, 818, 834, 1118
MslI CAYNNNNRTG 1 cut(s) 306
MspA1I CMGCKG 1 cut(s) 1109
MspCI CTTAAG 1 cut(s) 680
MspI CCGG 3 cut(s) 381, 571, 894
MspR9I CCNGG 3 cut(s) 571, 894, 903
MunI CAATTG 1 cut(s) 416
MvaI CCWGG 1 cut(s) 903
MvnI CGCG 2 cut(s) 33, 257
MwoI GCNNNNNNNGC 4 cut(s) 803, 903, 925, 973
NciI CCSGG 2 cut(s) 571, 894
NlaIII CATG 9 cut(s) 31, 167, 668, 785, 825, 1073, 1153, 1204, 1319
NlaIV GGNNCC 1 cut(s) 1020
NmuCI GTSAC 6 cut(s) 168, 308, 320, 383, 470, 767
NruI TCGCGA 1 cut(s) 257
NsiI ATGCAT 1 cut(s) 1317
PagI TCATGA 1 cut(s) 781
PciSI GCTCTTC 2 cut(s) 135, 508
PdmI GAANNNNTTC 1 cut(s) 1222
PfeI GAWTC 7 cut(s) 41, 155, 185, 536, 674, 732, 1137
PfoI TCCNGGA 1 cut(s) 569
PkrI GCNGC 5 cut(s) 35, 581, 756, 796, 1111
PleI GAGTC 3 cut(s) 175, 467, 601
PpsI GAGTC 3 cut(s) 175, 467, 601
Psp124BI GAGCTC 1 cut(s) 503
Psp1406I AACGTT 1 cut(s) 1145
Psp6I CCWGG 1 cut(s) 901
PspGI CCWGG 1 cut(s) 901
PspN4I GGNNCC 1 cut(s) 1020
PspPI GGNCC 2 cut(s) 273, 407
PstI CTGCAG 1 cut(s) 533
PstNI CAGNNNCTG 1 cut(s) 188
PsuI RGATCY 1 cut(s) 550
PvuII CAGCTG 1 cut(s) 1109
RruI TCGCGA 1 cut(s) 257
RsaI GTAC 1 cut(s) 765
RsaNI GTAC 1 cut(s) 764
RseI CAYNNNNRTG 1 cut(s) 306
SacI GAGCTC 1 cut(s) 503
SapI GCTCTTC 2 cut(s) 135, 508
SaqAI TTAA 6 cut(s) 131, 525, 681, 818, 834, 1118
SatI GCNGC 5 cut(s) 34, 580, 755, 795, 1110
Sau96I GGNCC 2 cut(s) 273, 407
SchI GAGTC 3 cut(s) 176, 467, 602
ScrFI CCNGG 3 cut(s) 571, 894, 903
SduI GDGCHC 2 cut(s) 503, 655
SfaNI GCATC 3 cut(s) 85, 878, 1205
SfcI CTRYAG 2 cut(s) 291, 529
SinI GGWCC 1 cut(s) 273
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 2 cut(s) 680, 1160
SmoI CTYRAG 2 cut(s) 680, 1160
SsiI CCGC 2 cut(s) 33, 755
SspMI CTAG 1 cut(s) 1002
SstI GAGCTC 1 cut(s) 503
StyD4I CCNGG 3 cut(s) 569, 892, 901
TaaI ACNGT 2 cut(s) 481, 604
TaiI ACGT 5 cut(s) 769, 804, 880, 1148, 1304
TaqI TCGA 4 cut(s) 6, 265, 707, 1298
TauI GCSGC 2 cut(s) 36, 757
TfiI GAWTC 7 cut(s) 41, 155, 185, 536, 674, 732, 1137
Tru1I TTAA 6 cut(s) 131, 525, 681, 818, 834, 1118
Tru9I TTAA 6 cut(s) 131, 525, 681, 818, 834, 1118
TscAI CASTG 3 cut(s) 228, 352, 550
TseFI GTSAC 6 cut(s) 168, 308, 320, 383, 470, 767
TseI GCWGC 3 cut(s) 579, 794, 1109
Tsp45I GTSAC 6 cut(s) 168, 308, 320, 383, 470, 767
TspGWI ACGGA 1 cut(s) 1149
TspRI CASTG 3 cut(s) 228, 352, 550
Vha464I CTTAAG 1 cut(s) 680
VpaK11BI GGWCC 1 cut(s) 273
XapI RAATTY 6 cut(s) 94, 391, 462, 810, 850, 1098
XmnI GAANNNNTTC 1 cut(s) 1222
XspI CTAG 1 cut(s) 1002
Zsp2I ATGCAT 1 cut(s) 1317
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.