Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0012830

Physical Location & Seq

Genomic Coordinates Forward (+)

451572 .. 456508

Gene Structure 4937 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1860 bp

ATGAGATCGGACGTAATCGGAGACGAAGAGAAATGGCTGGCGGAAGGCATCGCCGGCATTCAACACCATGCCTTTTACATGCATCGCGCTCTGGACGCCAACAATCTCAGAGACGCCCTCAAATTCTCGGCCCTGATGCTGTCGGAGCTCAGAACCTCTCGGCTTTCGCCTCACAAATACTACGATCTCTATATGCGTGCTTTCGATGAGCTGAGGAGGCTGGAGATGTTCTTCAAGGATGAGAGCAGACACGGCGTTTCGATCATCGATCTCTACGAACTTGTTCAGCATGCCGGCAACATTTTACCAAGATTGTATCTGCTATGTACGGTAGGATCCGTGTACATTAAATCTAAGGAAGCGCCGGCCAAGGACGTACTTAAAGATCTTGTCGAAATGTGTCGCGGCGTTCAGCATCCAATTCGAGGGCTCTTTTTAAGAAGCTATCTTTCTCAAGTCAGCAGAGACAAGTTGCCTGATATTGGTTCTGAGTATGAAGGGGATGCTGACACTGTCATGGATGCTGTAGATTTTGTGCTACAGAATTTTACAGAAATGAATAAGCTTTGGGTTCGAATGCAGTATCAGGGACCTGGTCATGTGAGAGAGAAGCGCGAAAAGGAAAGAAGCGAACTTCGTGATCTTGTTGGGAAAAATCTCCATGTTCTCAGTCAGATAGAGGGCGTGGAACTTGAAATGTACAAAGCCACTGTTCTTCCTAGAGTATTAGAACAGGTTATCAACTGTAAAGATGAGTTGGCCCAATATTATTTGATGGATTGCATAATCCAGGTCTTTCCAGATGAGTACCACTTGCAGACACTGGAGACATTGTTGGCTGCCTTCCCCCAGCTTCAGCCAACAGTTGATATCAAGACTGTGTTATCTCAATTAATGGAGAGGTTATCAAACTATGCTGCGTCAAGTACAGATGTATTACCTGAATTTCTTCAAGTGGAAGCCTTCGCTAAATTAAGCAGTGCCATTGGGAGGGTGATAGAAGCACAGATGGACATGCCTATTGTTGGAGCCATATCTTTGTATGTCTCTCTTCTTACATTTACCCTCCGTGTTCATCCTGATCGCCTTGATTATGTGGATCAAGTACTGGGAGCATGTGTTAAGAAGCTATCCGGAGAAACCAAGCTTGAAGACCGTAGAGCAACAAAACAGGTTGTTGCACTGTTAAGTTCTCCCTTGGAGAAATACGATGACATTGTCACAGCCCTCACACTTTCTAATTATCCTCGAGTTATGGAATATCTTGATAATGGAACAAATAAAGTCATGGCAATGGTCATTATCCAAAGCATTATGAAGAACAACTCTTGTATCTCCACAGCTGATAAGGTGGAGGTGTTGTTTGAATTAATTAATGGGCTCATCAAGGACTTGGATTCTACTTCTGCAGATGAGCTTGATGAAGAGGATTTTGTGGATGAACAAAATTCTGTTGCTCGCCTTATACACATGCTTTATGATGATGATCCAGAAGAAATGTTTAAGATCTTATGTACGGTCAAGAAGCATATAATGAGTGGAGGACCAAAGCGCCTACCTTTTACTGTTCCTCCTCTTATATTATCTGCACTCAAGTTGGTTAGGCGGCTGCAAGGTCAGGATGGAGAGGTTGTGGGAGAAGAAATGCCTGCCACACCGAAGAAAATTTTTCAGACTTTAAATCAGATCATTGAATCTCTTTCCTCAGTTCCGTCTCCCGAACTGGCCTTAAGATTGTACTTGGAATGTGCTGAGGCTGCTAATGACTGTGATCTGGAACCTGTTGCTTATGAATTCTTCACTCAGGCATTTATATTATATGAAGAAGAAGTTGCGTTTAGGATTCCAAAGCCCAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0005215 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0006810 GO:0006886 GO:0008104 GO:0008150 GO:0008565 GO:0012505 GO:0015031 GO:0015833 GO:0016020 GO:0016192 GO:0016197 GO:0016482 GO:0030904 GO:0031410 GO:0031982 GO:0032991 GO:0033036 GO:0034613 GO:0042147 GO:0042886 GO:0043226 GO:0043227 GO:0043229 GO:0044424 GO:0044425 GO:0044444 GO:0044464 GO:0045184 GO:0046907 GO:0051179 GO:0051234 GO:0051641 GO:0051649 GO:0070727 GO:0071702 GO:0071705 GO:0097708 GO:0098796

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

Vps35

Protein Analysis

620

Amino Acids

70.5

Weight (kDa)

5.02

Isoelectric Point (pI)

47.12

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Vps35	PF03635	12 - 611	8.2e-234	Vacuolar protein sorting-associated protein 35

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0012830)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G75850 AT1G75850
fragaria_vesca	FvH4_2g38180 FvH4_2g38180
malus_domestica	MD15G1010200.v1.1
prunus_persica	Prupe.1G362700_v2.0.a1
pyrus_communis	pycom15g00840
rosa_chinensis	RchiOBHm_Chr6g0303301
rosa_laevigata	RLG00000011094
rosa_multiflora	Rmu_sc0006346.1_g000001 Rmu_sc0019430.1_g000001
rosa_roxburghii	Rroxscaffold_7G00164890
rosa_rugosa	Rorug06G0320200
rosa_samantha	Rh6AG434600 Rh6BG468500 Rh6CG446600 Rh6DG433300
rosa_wichuraiana	Rw6G037630

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	3 cut(s)	87, 405, 615
AccIII	TCCGGA	1 cut(s)	1133
AciI	CCGC	3 cut(s)	41, 405, 1606
AclWI	GGATC	4 cut(s)	330, 343, 1107, 1481
AcoI	YGGCCR	1 cut(s)	366
AcsI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
AcuI	CTGAAG	1 cut(s)	839
AcyI	GRCGYC	2 cut(s)	96, 114
AfaI	GTAC	9 cut(s)	328, 344, 378, 701, 809, 928, 1107, 1516, 1739
AfiI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
AflII	CTTAAG	1 cut(s)	1729
AgsI	TTSAA	7 cut(s)	62, 235, 695, 953, 1151, 1367, 1694
AjnI	CCWGG	3 cut(s)	592, 789, 1853
AluBI	AGCT	9 cut(s)	148, 211, 444, 565, 853, 1129, 1147, 1343, 1417
AluI	AGCT	9 cut(s)	148, 211, 444, 565, 853, 1129, 1147, 1343, 1417
Alw21I	GWGCWC	1 cut(s)	150
Alw26I	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
AlwI	GGATC	4 cut(s)	330, 343, 1107, 1481
AlwNI	CAGNNNCTG	1 cut(s)	823
Ama87I	CYCGRG	1 cut(s)	1248
Aor13HI	TCCGGA	1 cut(s)	1133
AoxI	GGCC	4 cut(s)	129, 366, 759, 1725
ApeKI	GCWGC	4 cut(s)	839, 917, 1609, 1757
ApoI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
AseI	ATTAAT	3 cut(s)	893, 1370, 1374
Asp700I	GAANNNNTTC	2 cut(s)	282, 948
AspLEI	GCGC	4 cut(s)	89, 364, 615, 1554
AspS9I	GGNCC	4 cut(s)	130, 590, 760, 1544
AsuHPI	GGTGA	1 cut(s)	1006
AsuII	TTCGAA	1 cut(s)	574
AvaI	CYCGRG	1 cut(s)	1248
AvaII	GGWCC	2 cut(s)	590, 1544
BaeI	ACNNNNGTAYC	2 cut(s)	1315, 1348
BamHI	GGATCC	1 cut(s)	335
BanII	GRGCYC	3 cut(s)	150, 432, 1383
BbsI	GAAGAC	1 cut(s)	1158
Bbv12I	GWGCWC	1 cut(s)	150
BbvCI	CCTCAGC	2 cut(s)	212, 1752
BbvI	GCAGC	4 cut(s)	826, 904, 1596, 1744
BccI	CCATC	3 cut(s)	769, 1003, 1616
BceAI	ACGGC	1 cut(s)	268
BcgI	CGANNNNNNTGC	2 cut(s)	404, 438
BciT130I	CCWGG	3 cut(s)	594, 791, 1855
BcoDI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BfaI	CTAG	1 cut(s)	720
BfmI	CTRYAG	3 cut(s)	525, 539, 1407
BfoI	RGCGCY	2 cut(s)	365, 1555
BfrI	CTTAAG	1 cut(s)	1729
BglII	AGATCT	2 cut(s)	385, 1506
BisI	GCNGC	6 cut(s)	406, 840, 918, 1607, 1610, 1758
BlsI	GCNGC	6 cut(s)	407, 841, 919, 1608, 1611, 1759
BmcAI	AGTACT	1 cut(s)	1107
Bme1390I	CCNGG	3 cut(s)	594, 791, 1855
Bme18I	GGWCC	2 cut(s)	590, 1544
BmeT110I	CYCGRG	1 cut(s)	1248
BmgT120I	GGNCC	4 cut(s)	130, 590, 760, 1544
BmiI	GGNNCC	4 cut(s)	337, 591, 1030, 1779
BmrFI	CCNGG	3 cut(s)	594, 791, 1855
BmrI	ACTGGG	1 cut(s)	1118
BmsI	GCATC	6 cut(s)	57, 91, 126, 424, 493, 511
BmuI	ACTGGG	1 cut(s)	1118
BpiI	GAAGAC	1 cut(s)	1158
BplI	GAGNNNNNCTC	2 cut(s)	102, 134
BpmI	CTGGAG	2 cut(s)	242, 845
Bpu10I	CCTNAGC	2 cut(s)	212, 1752
Bpu14I	TTCGAA	1 cut(s)	574
BpuEI	CTTGAG	2 cut(s)	438, 1577
Bsa29I	ATCGAT	1 cut(s)	267
BsaBI	GATNNNNATC	1 cut(s)	1485
BsaHI	GRCGYC	2 cut(s)	96, 114
BsaJI	CCNNGG	3 cut(s)	369, 1197, 1853
BsaWI	WCCGGW	1 cut(s)	1133
Bsc4I	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
Bse118I	RCCGGY	3 cut(s)	53, 293, 364
Bse1I	ACTGG	3 cut(s)	828, 1113, 1728
Bse3DI	GCAATG	1 cut(s)	1299
Bse8I	GATNNNNATC	1 cut(s)	1485
BseAI	TCCGGA	1 cut(s)	1133
BseBI	CCWGG	3 cut(s)	594, 791, 1855
BseCI	ATCGAT	1 cut(s)	267
BseDI	CCNNGG	3 cut(s)	369, 1197, 1853
BseGI	GGATG	7 cut(s)	244, 415, 508, 526, 1075, 1444, 1627
BseJI	GATNNNNATC	1 cut(s)	1485
BseLI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
BseMI	GCAATG	1 cut(s)	1299
BseMII	CTCAG	8 cut(s)	121, 163, 203, 480, 682, 1719, 1743, 1817
BseNI	ACTGG	3 cut(s)	828, 1113, 1728
BseRI	GAGGAG	2 cut(s)	229, 1563
BseXI	GCAGC	4 cut(s)	826, 904, 1596, 1744
BseYI	CCCAGC	1 cut(s)	849
BsgI	GTGCAG	1 cut(s)	1572
Bsh1236I	CGCG	3 cut(s)	87, 405, 615
BshFI	GGCC	4 cut(s)	131, 368, 761, 1727
BshVI	ATCGAT	1 cut(s)	267
BsiHKAI	GWGCWC	1 cut(s)	150
BsiHKCI	CYCGRG	1 cut(s)	1248
BsiSI	CCGG	4 cut(s)	54, 294, 365, 1134
BslFI	GGGAC	1 cut(s)	603
BslI	CCNNNNNNNGG	4 cut(s)	425, 482, 990, 1025
BsmAI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BsmBI	CGTCTC	3 cut(s)	15, 105, 1719
BsmFI	GGGAC	1 cut(s)	603
BsmI	GAATGC	2 cut(s)	57, 582
BsnI	GGCC	4 cut(s)	131, 368, 761, 1727
BsoBI	CYCGRG	1 cut(s)	1248
Bsp119I	TTCGAA	1 cut(s)	574
Bsp1286I	GDGCHC	3 cut(s)	150, 432, 1383
Bsp13I	TCCGGA	1 cut(s)	1133
Bsp1407I	TGTACA	2 cut(s)	342, 699
BspACI	CCGC	3 cut(s)	41, 405, 1606
BspANI	GGCC	4 cut(s)	131, 368, 761, 1727
BspCNI	CTCAG	8 cut(s)	120, 162, 204, 481, 681, 1718, 1744, 1816
BspDI	ATCGAT	1 cut(s)	267
BspEI	TCCGGA	1 cut(s)	1133
BspFNI	CGCG	3 cut(s)	87, 405, 615
BspLI	GGNNCC	4 cut(s)	337, 591, 1030, 1779
BspMAI	CTGCAG	1 cut(s)	1411
BspPI	GGATC	4 cut(s)	330, 343, 1107, 1481
BspT104I	TTCGAA	1 cut(s)	574
BspTI	CTTAAG	1 cut(s)	1729
BsrDI	GCAATG	1 cut(s)	1299
BsrFI	RCCGGY	3 cut(s)	53, 293, 364
BsrGI	TGTACA	2 cut(s)	342, 699
BsrI	ACTGG	3 cut(s)	828, 1113, 1728
BssAI	RCCGGY	3 cut(s)	53, 293, 364
BssECI	CCNNGG	3 cut(s)	369, 1197, 1853
BssNI	GRCGYC	2 cut(s)	96, 114
BssT1I	CCWWGG	2 cut(s)	369, 1197
Bst2UI	CCWGG	3 cut(s)	594, 791, 1855
Bst6I	CTCTTC	3 cut(s)	21, 1056, 1419
BstACI	GRCGYC	2 cut(s)	96, 114
BstAFI	CTTAAG	1 cut(s)	1729
BstAUI	TGTACA	2 cut(s)	342, 699
BstBI	TTCGAA	1 cut(s)	574
BstC8I	GCNNGC	8 cut(s)	39, 55, 198, 291, 295, 366, 1459, 1650
BstDEI	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
BstF5I	GGATG	7 cut(s)	244, 415, 508, 526, 1075, 1444, 1627
BstFNI	CGCG	3 cut(s)	87, 405, 615
BstH2I	RGCGCY	2 cut(s)	365, 1555
BstHHI	GCGC	4 cut(s)	89, 364, 615, 1554
BstMAI	GTCTC	6 cut(s)	15, 105, 459, 821, 1051, 1719
BstMWI	GCNNNNNNNGC	6 cut(s)	54, 95, 145, 217, 252, 1757
BstNI	CCWGG	3 cut(s)	594, 791, 1855
BstNSI	RCATGY	5 cut(s)	82, 293, 1018, 1119, 1474
BstSCI	CCNGG	3 cut(s)	592, 789, 1853
BstSFI	CTRYAG	3 cut(s)	525, 539, 1407
BstUI	CGCG	3 cut(s)	87, 405, 615
BstV1I	GCAGC	4 cut(s)	826, 904, 1596, 1744
BstV2I	GAAGAC	1 cut(s)	1158
BstX2I	RGATCY	3 cut(s)	335, 385, 1506
BstYI	RGATCY	3 cut(s)	335, 385, 1506
Bsu15I	ATCGAT	1 cut(s)	267
BsuRI	GGCC	4 cut(s)	131, 368, 761, 1727
BsuTUI	ATCGAT	1 cut(s)	267
BtgZI	GCGATG	2 cut(s)	34, 68
BtsCI	GGATG	7 cut(s)	244, 415, 508, 526, 1075, 1444, 1627
BtsI	GCAGTG	1 cut(s)	985
BtsIMutI	CAGTG	5 cut(s)	510, 708, 821, 985, 1181
Cac8I	GCNNGC	8 cut(s)	39, 55, 198, 291, 295, 366, 1459, 1650
CaiI	CAGNNNCTG	1 cut(s)	823
CfoI	GCGC	4 cut(s)	89, 364, 615, 1554
Cfr10I	RCCGGY	3 cut(s)	53, 293, 364
Cfr13I	GGNCC	4 cut(s)	130, 590, 760, 1544
ClaI	ATCGAT	1 cut(s)	267
CseI	GACGC	3 cut(s)	104, 122, 909
CsiI	ACCWGGT	1 cut(s)	592
Csp6I	GTAC	9 cut(s)	327, 343, 377, 700, 808, 927, 1106, 1515, 1738
CviQI	GTAC	9 cut(s)	327, 343, 377, 700, 808, 927, 1106, 1515, 1738
DdeI	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
DraI	TTTAAA	1 cut(s)	1680
EaeI	YGGCCR	1 cut(s)	366
Eam1104I	CTCTTC	3 cut(s)	21, 1056, 1419
EarI	CTCTTC	3 cut(s)	21, 1056, 1419
EciI	GGCGGA	1 cut(s)	56
Ecl136II	GAGCTC	1 cut(s)	148
Eco130I	CCWWGG	2 cut(s)	369, 1197
Eco24I	GRGCYC	3 cut(s)	150, 432, 1383
Eco32I	GATATC	1 cut(s)	871
Eco47I	GGWCC	2 cut(s)	590, 1544
Eco53kI	GAGCTC	1 cut(s)	148
Eco57I	CTGAAG	1 cut(s)	839
Eco88I	CYCGRG	1 cut(s)	1248
EcoICRI	GAGCTC	1 cut(s)	148
EcoO109I	RGGNCCY	1 cut(s)	590
EcoRI	GAATTC	1 cut(s)	1793
EcoRII	CCWGG	3 cut(s)	592, 789, 1853
EcoRV	GATATC	1 cut(s)	871
EcoT14I	CCWWGG	2 cut(s)	369, 1197
EcoT22I	ATGCAT	1 cut(s)	84
EcoT38I	GRGCYC	3 cut(s)	150, 432, 1383
ErhI	CCWWGG	2 cut(s)	369, 1197
Esp3I	CGTCTC	3 cut(s)	15, 105, 1719
FaqI	GGGAC	1 cut(s)	603
Fnu4HI	GCNGC	6 cut(s)	406, 840, 918, 1607, 1610, 1758
FokI	GGATG	7 cut(s)	251, 402, 515, 533, 1062, 1451, 1634
FriOI	GRGCYC	3 cut(s)	150, 432, 1383
Fsp4HI	GCNGC	6 cut(s)	406, 840, 918, 1607, 1610, 1758
FspBI	CTAG	1 cut(s)	720
GlaI	GCGC	4 cut(s)	88, 363, 614, 1553
GluI	GCNGC	6 cut(s)	406, 840, 918, 1607, 1610, 1758
GsaI	CCCAGC	1 cut(s)	853
GsuI	CTGGAG	2 cut(s)	242, 845
HaeII	RGCGCY	2 cut(s)	365, 1555
HaeIII	GGCC	4 cut(s)	131, 368, 761, 1727
HapII	CCGG	4 cut(s)	54, 294, 365, 1134
HgaI	GACGC	3 cut(s)	104, 122, 909
HhaI	GCGC	4 cut(s)	89, 364, 615, 1554
Hin1I	GRCGYC	2 cut(s)	96, 114
Hin6I	GCGC	4 cut(s)	87, 362, 613, 1552
HinP1I	GCGC	4 cut(s)	87, 362, 613, 1552
HindIII	AAGCTT	2 cut(s)	563, 1145
HinfI	GANTC	3 cut(s)	1397, 1694, 1843
HpaII	CCGG	4 cut(s)	54, 294, 365, 1134
HphI	GGTGA	1 cut(s)	1006
Hpy166II	GTNNAC	1 cut(s)	343
Hpy188I	TCNGA	9 cut(s)	10, 20, 110, 145, 152, 490, 675, 1674, 1686
Hpy8I	GTNNAC	1 cut(s)	343
HpyAV	CCTTC	4 cut(s)	38, 491, 853, 973
HpyCH4IV	ACGT	2 cut(s)	12, 375
HpyCH4V	TGCA	8 cut(s)	82, 580, 783, 817, 1181, 1409, 1589, 1612
HpyF10VI	GCNNNNNNNGC	6 cut(s)	54, 95, 145, 217, 252, 1757
HpyF3I	CTNAG	9 cut(s)	107, 149, 212, 354, 489, 668, 1705, 1752, 1803
HpySE526I	ACGT	2 cut(s)	12, 375
Hsp92I	GRCGYC	2 cut(s)	96, 114
HspAI	GCGC	4 cut(s)	87, 362, 613, 1552
Kpn2I	TCCGGA	1 cut(s)	1133
KroI	GCCGGC	3 cut(s)	53, 293, 364
KroNI	GCCGGC	3 cut(s)	55, 295, 366
LmnI	GCTCC	3 cut(s)	145, 1028, 1112
Lsp1109I	GCAGC	4 cut(s)	826, 904, 1596, 1744
LweI	GCATC	6 cut(s)	57, 91, 126, 424, 493, 511
MabI	ACCWGGT	1 cut(s)	592
MaeI	CTAG	1 cut(s)	720
MaeII	ACGT	2 cut(s)	12, 375
MaeIII	GTNAC	1 cut(s)	1219
MflI	RGATCY	3 cut(s)	335, 385, 1506
MhlI	GDGCHC	3 cut(s)	150, 432, 1383
MmeI	TCCRAC	2 cut(s)	123, 1006
Mph1103I	ATGCAT	1 cut(s)	84
MroI	TCCGGA	1 cut(s)	1133
MroNI	GCCGGC	3 cut(s)	53, 293, 364
MroXI	GAANNNNTTC	2 cut(s)	282, 948
MslI	CAYNNNNRTG	2 cut(s)	515, 1292
MspA1I	CMGCKG	1 cut(s)	1343
MspCI	CTTAAG	1 cut(s)	1729
MspI	CCGG	4 cut(s)	54, 294, 365, 1134
MspR9I	CCNGG	3 cut(s)	594, 791, 1855
Mva1269I	GAATGC	2 cut(s)	57, 582
MvaI	CCWGG	3 cut(s)	594, 791, 1855
MvnI	CGCG	3 cut(s)	87, 405, 615
MwoI	GCNNNNNNNGC	6 cut(s)	54, 95, 145, 217, 252, 1757
NaeI	GCCGGC	3 cut(s)	55, 295, 366
NgoMIV	GCCGGC	3 cut(s)	53, 293, 364
NlaIV	GGNNCC	4 cut(s)	337, 591, 1030, 1779
NmeAIII	GCCGAG	2 cut(s)	107, 139
NmuCI	GTSAC	1 cut(s)	1219
NsiI	ATGCAT	1 cut(s)	84
NspI	RCATGY	5 cut(s)	82, 293, 1018, 1119, 1474
NspV	TTCGAA	1 cut(s)	574
PacI	TTAATTAA	1 cut(s)	1374
PaeI	GCATGC	1 cut(s)	293
PaeR7I	CTCGAG	1 cut(s)	1248
PcsI	WCGNNNNNNNCGW	1 cut(s)	273
PctI	GAATGC	2 cut(s)	57, 582
PdiI	GCCGGC	3 cut(s)	55, 295, 366
PdmI	GAANNNNTTC	2 cut(s)	282, 948
PfeI	GAWTC	3 cut(s)	1397, 1694, 1843
PflFI	GACNNNGTC	3 cut(s)	512, 594, 1217
PkrI	GCNGC	6 cut(s)	407, 841, 919, 1608, 1611, 1759
PpuMI	RGGWCCY	1 cut(s)	590
PshBI	ATTAAT	3 cut(s)	893, 1370, 1374
Psp124BI	GAGCTC	1 cut(s)	150
Psp5II	RGGWCCY	1 cut(s)	590
Psp6I	CCWGG	3 cut(s)	592, 789, 1853
PspFI	CCCAGC	1 cut(s)	849
PspGI	CCWGG	3 cut(s)	592, 789, 1853
PspN4I	GGNNCC	4 cut(s)	337, 591, 1030, 1779
PspPI	GGNCC	4 cut(s)	130, 590, 760, 1544
PspPPI	RGGWCCY	1 cut(s)	590
PspXI	VCTCGAGB	1 cut(s)	1248
PstI	CTGCAG	1 cut(s)	1411
PstNI	CAGNNNCTG	1 cut(s)	823
PsuI	RGATCY	3 cut(s)	335, 385, 1506
PsyI	GACNNNGTC	3 cut(s)	512, 594, 1217
PvuII	CAGCTG	1 cut(s)	1343
RsaI	GTAC	9 cut(s)	328, 344, 378, 701, 809, 928, 1107, 1516, 1739
RsaNI	GTAC	9 cut(s)	327, 343, 377, 700, 808, 927, 1106, 1515, 1738
RseI	CAYNNNNRTG	2 cut(s)	515, 1292
SacI	GAGCTC	1 cut(s)	150
SatI	GCNGC	6 cut(s)	406, 840, 918, 1607, 1610, 1758
Sau96I	GGNCC	4 cut(s)	130, 590, 760, 1544
ScaI	AGTACT	1 cut(s)	1107
ScrFI	CCNGG	3 cut(s)	594, 791, 1855
SduI	GDGCHC	3 cut(s)	150, 432, 1383
SexAI	ACCWGGT	1 cut(s)	592
SfaNI	GCATC	6 cut(s)	57, 91, 126, 424, 493, 511
SfcI	CTRYAG	3 cut(s)	525, 539, 1407
Sfr274I	CTCGAG	1 cut(s)	1248
SfuI	TTCGAA	1 cut(s)	574
SinI	GGWCC	2 cut(s)	590, 1544
SlaI	CTCGAG	1 cut(s)	1248
SmiMI	CAYNNNNRTG	2 cut(s)	515, 1292
SmlI	CTYRAG	4 cut(s)	453, 1248, 1592, 1729
SmoI	CTYRAG	4 cut(s)	453, 1248, 1592, 1729
SphI	GCATGC	1 cut(s)	293
SsiI	CCGC	3 cut(s)	41, 405, 1606
SspI	AATATT	1 cut(s)	767
SspMI	CTAG	1 cut(s)	720
SstI	GAGCTC	1 cut(s)	150
StyD4I	CCNGG	3 cut(s)	592, 789, 1853
StyI	CCWWGG	2 cut(s)	369, 1197
TaiI	ACGT	2 cut(s)	15, 378
TaqI	TCGA	7 cut(s)	204, 260, 267, 393, 424, 574, 1249
TatI	WGTACW	5 cut(s)	342, 699, 926, 1105, 1737
TauI	GCSGC	2 cut(s)	408, 1609
TfiI	GAWTC	3 cut(s)	1397, 1694, 1843
TscAI	CASTG	5 cut(s)	517, 715, 828, 985, 1188
TseFI	GTSAC	1 cut(s)	1219
TseI	GCWGC	4 cut(s)	839, 917, 1609, 1757
Tsp45I	GTSAC	1 cut(s)	1219
TspDTI	ATGAA	8 cut(s)	510, 572, 1064, 1331, 1437, 1455, 1806, 1836
TspGWI	ACGGA	3 cut(s)	328, 1058, 1701
TspRI	CASTG	5 cut(s)	517, 715, 828, 985, 1188
Tth111I	GACNNNGTC	3 cut(s)	512, 594, 1217
Vha464I	CTTAAG	1 cut(s)	1729
VpaK11BI	GGWCC	2 cut(s)	590, 1544
VspI	ATTAAT	3 cut(s)	893, 1370, 1374
XapI	RAATTY	6 cut(s)	122, 544, 944, 1447, 1665, 1793
XceI	RCATGY	5 cut(s)	82, 293, 1018, 1119, 1474
XhoI	CTCGAG	1 cut(s)	1248
XmnI	GAANNNNTTC	2 cut(s)	282, 948
XspI	CTAG	1 cut(s)	720
ZrmI	AGTACT	1 cut(s)	1107
Zsp2I	ATGCAT	1 cut(s)	84

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.