pycom15g02120

Gamma carbonic anhydrase 1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0012857
Physical Location & Seq
Reverse (-)
1254369 .. 1256304
1936 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 684 bp
ATGAACGTATTCGATAAAGCTCCTGTGGTTGACAAGGATGTATTCGTGGCGCCAAGTGCCTCCATCATTGGCGACGTTCAAGTGGGAAGAGGGTCTTCCATATGGTATGGATGTGTTTTGAGAGGTGATGTGAACAACATCGTTGTTGGATCTGGAACTAACATACAGGACAACTCCCTTGTTCATGTGGCGAAGTCAAATTTAAGTGGGAAGGTGTTACCAACCATTATTGGAGATAATGTTACTGTAGGTCACAGTGCTGTTATACATGGCTGTACTGTTGAGGATGAGGCCTTTGTTGGCATGGGAGCCACACTTCTTGATGGCGTCGTCGTTGAGAAACATGCCATGGTAGCTGCTGGATCCCTGGTGAGACAGAATACAAAAATCCCCAGCGGAGAGGTATGGGCAGGAAACCCAGCAAAATTTCTGAGAAAGCTCACAGATGAAGAAATAGCCTTCATCTCCCAGTCAGCCACCAATTACACCAACCTTGCACAAGTCCATGCAGCTGAGAATGTGAAGTCGTTTGATGAGATTGAGTTTGAGAAGGCCCTCCGCAAGAAGTTTGCCCACAAGGATGAGGAGTATGACTCAATGCTAGGTGTCGTTCGTGAGATTCCCCCAGAACTTATTCTTCCCGATAATGTATTACCAGATAAAGCAGCAAAGGACCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Hexapep

Protein Analysis

228

Amino Acids

24.37

Weight (kDa)

5.24

Isoelectric Point (pI)

31.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Hexapep PF00132 9 - 42 6e-06 Bacterial transferase hexapeptide (six repeats)
LbH_EIF2B PF25084 45 - 131 1.3e-06 EIF2B subunit epsilon LbH domain
Hexapep PF00132 74 - 106 5.3e-06 Bacterial transferase hexapeptide (six repeats)
Hexapep_2 PF14602 74 - 106 2e-06 Hexapeptide repeat of succinyl-transferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012857)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47260
fragaria_vesca FvH4_2g39590
malus_domestica MD08G1029100.v1.1 MD15G1024600.v1.1
prunus_persica Prupe.1G376900_v2.0.a1 Prupe.1G376900_v2.0.a1
pyrus_communis pycom08g02290 pycom15g02120
rosa_chinensis RchiOBHm_Chr6g0301331
rosa_multiflora Rmu_sc0003197.1_g000011 Rmu_sc0013354.1_g000021
rosa_roxburghii Rroxscaffold_7G00166910
rosa_rugosa Rorug06G0303700 Rorug07G0031300
rosa_samantha Rh6BG423900 Rh6CG431100 Rh6DG417000
rosa_wichuraiana Rw6G036110

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 49
AciI CCGC 2 cut(s) 396, 559
AclWI GGATC 3 cut(s) 157, 357, 370
AcsI RAATTY 2 cut(s) 199, 425
AcyI GRCGYC 2 cut(s) 50, 327
AfaI GTAC 1 cut(s) 277
AgsI TTSAA 1 cut(s) 80
AjnI CCWGG 1 cut(s) 366
AluBI AGCT 4 cut(s) 20, 356, 439, 512
AluI AGCT 4 cut(s) 20, 356, 439, 512
Alw26I GTCTC 1 cut(s) 367
AlwI GGATC 3 cut(s) 157, 357, 370
AoxI GGCC 2 cut(s) 291, 552
ApeKI GCWGC 3 cut(s) 356, 509, 665
ApoI RAATTY 2 cut(s) 199, 425
Asp700I GAANNNNTTC 2 cut(s) 8, 633
AspLEI GCGC 1 cut(s) 52
AspS9I GGNCC 2 cut(s) 553, 673
AsuHPI GGTGA 2 cut(s) 137, 382
AvaII GGWCC 1 cut(s) 673
BamHI GGATCC 1 cut(s) 362
BanI GGYRCC 1 cut(s) 49
BbsI GAAGAC 1 cut(s) 87
BbvI GCAGC 3 cut(s) 343, 521, 677
BccI CCATC 2 cut(s) 71, 317
BciT130I CCWGG 1 cut(s) 368
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 602
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 53
BisI GCNGC 3 cut(s) 357, 510, 666
BlsI GCNGC 3 cut(s) 358, 511, 667
Bme1390I CCNGG 1 cut(s) 368
Bme18I GGWCC 1 cut(s) 673
BmgT120I GGNCC 2 cut(s) 553, 673
BmiI GGNNCC 3 cut(s) 51, 310, 364
BmrFI CCNGG 1 cut(s) 368
BmrI ACTGGG 1 cut(s) 463
BmuI ACTGGG 1 cut(s) 463
BpiI GAAGAC 1 cut(s) 87
BplI GAGNNNNNCTC 2 cut(s) 578, 610
BsaHI GRCGYC 2 cut(s) 50, 327
BsaJI CCNNGG 2 cut(s) 348, 366
BsaXI ACNNNNNCTCC 2 cut(s) 225, 255
Bse1I ACTGG 1 cut(s) 469
BseBI CCWGG 1 cut(s) 368
BseDI CCNNGG 2 cut(s) 348, 366
BseGI GGATG 4 cut(s) 43, 116, 292, 586
BseMII CTCAG 2 cut(s) 422, 504
BseNI ACTGG 1 cut(s) 469
BseRI GAGGAG 1 cut(s) 599
BseXI GCAGC 3 cut(s) 343, 521, 677
BseYI CCCAGC 2 cut(s) 392, 418
BshFI GGCC 2 cut(s) 293, 554
BshNI GGYRCC 1 cut(s) 49
BsmAI GTCTC 1 cut(s) 367
BsnI GGCC 2 cut(s) 293, 554
Bsp143I GATC 2 cut(s) 149, 362
Bsp19I CCATGG 1 cut(s) 348
BspACI CCGC 2 cut(s) 396, 559
BspANI GGCC 2 cut(s) 293, 554
BspCNI CTCAG 2 cut(s) 423, 505
BspLI GGNNCC 3 cut(s) 51, 310, 364
BspPI GGATC 3 cut(s) 157, 357, 370
BspT107I GGYRCC 1 cut(s) 49
BsrI ACTGG 1 cut(s) 469
BssECI CCNNGG 2 cut(s) 348, 366
BssMI GATC 2 cut(s) 149, 362
BssNI GRCGYC 2 cut(s) 50, 327
BssT1I CCWWGG 1 cut(s) 348
Bst2UI CCWGG 1 cut(s) 368
Bst4CI ACNGT 3 cut(s) 247, 257, 280
Bst6I CTCTTC 1 cut(s) 82
BstACI GRCGYC 2 cut(s) 50, 327
BstDEI CTNAG 2 cut(s) 431, 513
BstDSI CCRYGG 1 cut(s) 348
BstF5I GGATG 4 cut(s) 43, 116, 292, 586
BstH2I RGCGCY 1 cut(s) 53
BstHHI GCGC 1 cut(s) 52
BstKTI GATC 2 cut(s) 152, 365
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 2 cut(s) 149, 362
BstMWI GCNNNNNNNGC 2 cut(s) 56, 353
BstNI CCWGG 1 cut(s) 368
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 1 cut(s) 366
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 343, 521, 677
BstV2I GAAGAC 1 cut(s) 87
BstX2I RGATCY 2 cut(s) 149, 362
BstYI RGATCY 2 cut(s) 149, 362
BsuRI GGCC 2 cut(s) 293, 554
BtgI CCRYGG 1 cut(s) 348
BtsCI GGATG 4 cut(s) 43, 116, 292, 586
BtsIMutI CAGTG 1 cut(s) 262
CfoI GCGC 1 cut(s) 52
Cfr13I GGNCC 2 cut(s) 553, 673
CseI GACGC 1 cut(s) 316
Csp6I GTAC 1 cut(s) 276
CspCI CAANNNNNGTGG 2 cut(s) 301, 336
CviAII CATG 6 cut(s) 185, 269, 304, 344, 349, 506
CviQI GTAC 1 cut(s) 276
DdeI CTNAG 2 cut(s) 431, 513
DinI GGCGCC 1 cut(s) 51
DpnI GATC 2 cut(s) 151, 364
DpnII GATC 2 cut(s) 149, 362
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco130I CCWWGG 1 cut(s) 348
Eco147I AGGCCT 1 cut(s) 293
Eco47I GGWCC 1 cut(s) 673
EcoO109I RGGNCCY 1 cut(s) 553
EcoRII CCWGG 1 cut(s) 366
EcoT14I CCWWGG 1 cut(s) 348
EgeI GGCGCC 1 cut(s) 51
EheI GGCGCC 1 cut(s) 51
ErhI CCWWGG 1 cut(s) 348
FaeI CATG 6 cut(s) 188, 272, 307, 347, 352, 509
FalI AAGNNNNNCTT 2 cut(s) 79, 111
FatI CATG 6 cut(s) 184, 268, 303, 343, 348, 505
FauNDI CATATG 1 cut(s) 101
Fnu4HI GCNGC 3 cut(s) 357, 510, 666
FokI GGATG 4 cut(s) 50, 123, 299, 593
Fsp4HI GCNGC 3 cut(s) 357, 510, 666
FspBI CTAG 1 cut(s) 602
GlaI GCGC 1 cut(s) 51
GluI GCNGC 3 cut(s) 357, 510, 666
GsaI CCCAGC 2 cut(s) 396, 422
HaeII RGCGCY 1 cut(s) 53
HaeIII GGCC 2 cut(s) 293, 554
HgaI GACGC 1 cut(s) 316
HhaI GCGC 1 cut(s) 52
Hin1I GRCGYC 2 cut(s) 50, 327
Hin1II CATG 6 cut(s) 188, 272, 307, 347, 352, 509
Hin6I GCGC 1 cut(s) 50
HinP1I GCGC 1 cut(s) 50
HincII GTYRAC 1 cut(s) 31
HindII GTYRAC 1 cut(s) 31
HinfI GANTC 2 cut(s) 593, 619
HphI GGTGA 2 cut(s) 137, 382
Hpy166II GTNNAC 2 cut(s) 31, 133
Hpy188I TCNGA 1 cut(s) 432
Hpy188III TCNNGA 4 cut(s) 153, 320, 614, 641
Hpy8I GTNNAC 2 cut(s) 31, 133
Hpy99I CGWCG 3 cut(s) 77, 332, 335
HpyAV CCTTC 3 cut(s) 205, 469, 544
HpyCH4III ACNGT 3 cut(s) 247, 257, 280
HpyCH4IV ACGT 2 cut(s) 6, 75
HpyCH4V TGCA 2 cut(s) 497, 509
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 353
HpyF3I CTNAG 2 cut(s) 431, 513
HpySE526I ACGT 2 cut(s) 6, 75
Hsp92I GRCGYC 2 cut(s) 50, 327
Hsp92II CATG 6 cut(s) 188, 272, 307, 347, 352, 509
HspAI GCGC 1 cut(s) 50
KasI GGCGCC 1 cut(s) 49
Kzo9I GATC 2 cut(s) 149, 362
LmnI GCTCC 2 cut(s) 25, 308
Lsp1109I GCAGC 3 cut(s) 343, 521, 677
MaeI CTAG 1 cut(s) 602
MaeII ACGT 2 cut(s) 6, 75
MaeIII GTNAC 3 cut(s) 216, 241, 251
MalI GATC 2 cut(s) 151, 364
MboI GATC 2 cut(s) 149, 362
MboII GAAGA 4 cut(s) 87, 99, 461, 629
MflI RGATCY 2 cut(s) 149, 362
MluCI AATT 3 cut(s) 199, 425, 481
Mly113I GGCGCC 1 cut(s) 50
MlyI GAGTC 1 cut(s) 587
MmeI TCCRAC 1 cut(s) 127
MnlI CCTC 8 cut(s) 70, 83, 116, 277, 283, 394, 566, 577
MroXI GAANNNNTTC 2 cut(s) 8, 633
MseI TTAA 1 cut(s) 203
MslI CAYNNNNRTG 1 cut(s) 579
MspA1I CMGCKG 2 cut(s) 396, 512
MspR9I CCNGG 1 cut(s) 368
MvaI CCWGG 1 cut(s) 368
MwoI GCNNNNNNNGC 2 cut(s) 56, 353
NarI GGCGCC 1 cut(s) 50
NcoI CCATGG 1 cut(s) 348
NdeI CATATG 1 cut(s) 101
NdeII GATC 2 cut(s) 149, 362
NlaIII CATG 6 cut(s) 188, 272, 307, 347, 352, 509
NlaIV GGNNCC 3 cut(s) 51, 310, 364
NmuCI GTSAC 1 cut(s) 251
NspI RCATGY 1 cut(s) 347
PceI AGGCCT 1 cut(s) 293
PdmI GAANNNNTTC 2 cut(s) 8, 633
PfeI GAWTC 1 cut(s) 619
PkrI GCNGC 3 cut(s) 358, 511, 667
PleI GAGTC 1 cut(s) 587
PluTI GGCGCC 1 cut(s) 53
PpsI GAGTC 1 cut(s) 587
Psp6I CCWGG 1 cut(s) 366
PspFI CCCAGC 2 cut(s) 392, 418
PspGI CCWGG 1 cut(s) 366
PspN4I GGNNCC 3 cut(s) 51, 310, 364
PspPI GGNCC 2 cut(s) 553, 673
PsuI RGATCY 2 cut(s) 149, 362
PvuII CAGCTG 1 cut(s) 512
RsaI GTAC 1 cut(s) 277
RsaNI GTAC 1 cut(s) 276
RseI CAYNNNNRTG 1 cut(s) 579
SaqAI TTAA 1 cut(s) 203
SatI GCNGC 3 cut(s) 357, 510, 666
Sau3AI GATC 2 cut(s) 149, 362
Sau96I GGNCC 2 cut(s) 553, 673
SchI GAGTC 1 cut(s) 587
ScrFI CCNGG 1 cut(s) 368
SfcI CTRYAG 1 cut(s) 246
SfoI GGCGCC 1 cut(s) 51
SinI GGWCC 1 cut(s) 673
SmiMI CAYNNNNRTG 1 cut(s) 579
Sse9I AATT 3 cut(s) 199, 425, 481
SseBI AGGCCT 1 cut(s) 293
SsiI CCGC 2 cut(s) 396, 559
SspDI GGCGCC 1 cut(s) 49
SspMI CTAG 1 cut(s) 602
StuI AGGCCT 1 cut(s) 293
StyD4I CCNGG 1 cut(s) 366
StyI CCWWGG 1 cut(s) 348
TaaI ACNGT 3 cut(s) 247, 257, 280
TaiI ACGT 2 cut(s) 9, 78
TaqI TCGA 1 cut(s) 12
TasI AATT 3 cut(s) 199, 425, 481
TatI WGTACW 1 cut(s) 275
TfiI GAWTC 1 cut(s) 619
Tru1I TTAA 1 cut(s) 203
Tru9I TTAA 1 cut(s) 203
TscAI CASTG 1 cut(s) 262
TseFI GTSAC 1 cut(s) 251
TseI GCWGC 3 cut(s) 356, 509, 665
Tsp45I GTSAC 1 cut(s) 251
TspDTI ATGAA 4 cut(s) 17, 173, 451, 462
TspRI CASTG 1 cut(s) 262
VpaK11BI GGWCC 1 cut(s) 673
XapI RAATTY 2 cut(s) 199, 425
XceI RCATGY 1 cut(s) 347
XmnI GAANNNNTTC 2 cut(s) 8, 633
XspI CTAG 1 cut(s) 602
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.