Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr15

Orthogroup ID

OG0009089

Physical Location & Seq

Genomic Coordinates Reverse (-)

2872562 .. 2878014

Gene Structure 5453 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2652 bp

ATGATAAACAGTATGCTGAGGCTCTCTCCCTCCTCTGACCCCAAGCTCCTCTTCAGCTACACCACCAACTCCTCCAGTGTTGACCGCTTCGCTTTCAACTTCAAGCCCCACCACCTCCCCCAACGCCGCCGTTCCAACCACCTCCTCCGCCCACAATCAATCCCTAATTTCACCCTCCGCAGCTCCCTCCAGACCTCCGCCGACGCCGCGGCGCCACTTCAGCAAGTCCAGAATGATCCACCCGCCGAGGCATCGGTGCTGCTCGATGTCTCCGGGATGATGTGCGGCGGCTGCGTCTCGCGAGTCAAATCTATTCTCTCCGCCGACGACCGAGTCGACTCGGTAGCGGTCAACTTGCTGACCGAGACGGCGGCGATCAAGCTGAGGCCGGAGGTCGCCGCCGATGTCGCGGCAGAGAGCCTGGCGGGGAGGCTGACGGAGTGCGGATTCGCTTCCAAGAGGAGGGCTTCCGGGATGGGAGTGTCGGAGAGCGTGAGGAAGTGGAAGGAGACGGTGAATAAGAAGGAGGAGATGCTGGTGAAGAGCAGGAACAGAGTGATTTTAGCTTGGACTTTAGTGGCATTGTGCTGCGGATCGCATGCTTCTCACATTTTGCATTCTCTTGGAATTCATGCTGCTCATGGATCGTTTTGGGAGCTGCTGCATAATTCGTATGTGAAGGCGGGTTTAGCTACCGGAGCTTTGTTGGGACCTGGCCGAGACTTGCTTTTTGATGGTCTGAGAGCCTTAAAGAAAGGATCACCAAATATGAATTCTCTTGTGGGATTCGGATCCCTTGCTGCTTTTACAATTAGTGCGGTCTCTCTTCTCAACCCTGGGCTTCAATGGGATGCGGCGTTCTTCGATGAGCCGGTCATGCTTCTTGGTTTTGTGCTCCTAGGACGTTCTCTTGAGGAGAGGGCAAGGATCATGGCATCTAGTGATATGAATGAACTCTTGTCACTGATAAACACACAAGCAAGACTTGTAATTGGTTCGTCGGAAAATGATTCCTCCAGCAACAGTGTACTTTTCTCTGATGCAATTTGCGTTGAAGTCCCAACTGATGATATTCGAGTTGGAGACTCTGTGTTGGTTTTGCCAGGAGAAACCATACCTGTGGATGGAAGAGTTGTGGCTGGAAGAAGTGTTGTCGACGAATCCATGCTCACAGGAGAATCACTTCCTGTATTCAAGGAAAAAGACCTTACTGTCTCAGCTGGAACAATAAACTGGGATGGTCCTCTGCGGGTTGAAGCATCTTCAACTGGCTCCAATTCAATGATATCCAAGATCGTTCGCATGGTTGAGGATGCTCAAGGACATGAAGCGCCCATACAAAGGCTTGCTGATTCAATAGCCGGACCTTTTGTATACAGTATAATGACTCTATCAGCTACAACATTTGCTTTTTGGTACTACTTTGGAACACAAATGTTTCCAGATGTTTTGCTCAACGATATTGCTGGGCCAGATGGAGATCCATTGCTGTTGAGCTTGAAACTTGCTGTTGATGTCTTGGTTGTTTCTTGCCCTTGTGCACTGGGTCTTGCCACACCTACAGCAATCCTAGTTGGGACTTCACTCGGAGCAAGGCAGGGACTCCTTGTGAGAGGAGCAGATGTGCTCGAACGCTTAGCCAGCGTAGATTATATTGCTTTAGATAAGACAGGAACTCTTACAGAAGGAAAACCTGCTGTTTCTGGTATTGCATCTTCCATGTATGAAGAGTCCGAAATTCTTCAAATTGCTGCTGCAGTGGAGAATACAGCATCACATCCAATTGCAAGGGCTATCTTAAATAAAGCAAAATCATTGAACATGAGTATACCAATCACAAGAAGGCAATTAACAGAACCAGGTTTTGGAACTGTAGCAGAAGTAGATGGCCGATTGGTTGCAGTTGGTAGCTTGGAATGGGTCCATCAACGGTTCCAGGCAAAAGCCAATGTGTCTGATCTTTCAAATCTAGAACATACTGTGCGCCAATCATCAGAAGGCATAACACCTTCAAGTTATTCAAAAACGATTGTATATGTTGGTCGTGAAGGAGAAGGCATCATTGGTGCTATTGCAATATCTGATAGCCTGCGTCATGATGCTGAGTATACTCAAAAGGGTATCAGAACTGTCCTCTTTTCCGGAGACAGGGAAGAGGCAGTTGCAACTATAGCGAAAGCTGTTGGAATAGAAAATGAATTTGTCAAGTCATCATTGACTCCACAGGGAAAATCTGGAGCTATTTCTAGTCTTAAAGATGCAGGACATCACGTTGCAATGGTTGGCGATGGCATAAATGACGCACCATCTTTGGCACTTGCAGACGTTGGGATTGCTTTTCAGATTGAGGGGCAAGAAAATGCCGCTTCAAATGCTGCATCTATTATACTTCTCGGAAACAAATTATCTCAAGTTGTAGACGCCCTGGAACTGTCACAGGCAACAATGGCAAAAGTGTATCAAAATTTATCTTGGGCAATCGCATATAACTTATTTGCCATCCCCATTGCTGCCGGAGTACTGCTTCCTCAATATGATTTTGCCATGACACCATCACTCTCCGGCGGGATGATGGCCTTGAGCTCAATATTTGTAGTTTCCAATTCATTGCTTCTACAGCTCCATAAATCGGACGGGAGTAGAAAGAGTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000041 GO:0003674 GO:0005215 GO:0005375 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0006810 GO:0006811 GO:0006812 GO:0006825 GO:0008150 GO:0008324 GO:0009507 GO:0009534 GO:0009535 GO:0009536 GO:0009579 GO:0015075 GO:0015318 GO:0016020 GO:0022857 GO:0022890 GO:0030001 GO:0031976 GO:0031984 GO:0034220 GO:0034357 GO:0035434 GO:0042651 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044434 GO:0044435 GO:0044436 GO:0044444 GO:0044446 GO:0044464 GO:0046873 GO:0046915 GO:0051179 GO:0051234 GO:0055035 GO:0055085 GO:0098655 GO:0098660 GO:0098662

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

E1-E2_ATPase HMA Hydrolase

Protein Analysis

884

Amino Acids

93.86

Weight (kDa)

5.67

Isoelectric Point (pI)

34.64

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
HMA	PF00403	88 - 150	2.7e-09	Heavy-metal-associated domain
E1-E2_ATPase	PF00122	345 - 437	1.4e-27	P-type ATPase actuator domain
Hydrolase	PF00702	550 - 775	7.9e-20	haloacid dehalogenase-like hydrolase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009089)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G21930 AT5G21930 AT5G21930 AT5G21930
fragaria_vesca	FvH4_2g30930 FvH4_2g30930 FvH4_2g30930
malus_domestica	MD15G1049400.v1.1
prunus_persica	Prupe.1G403700_v2.0.a1 Prupe.1G403700_v2.0.a1
pyrus_communis	pycom15g04820
rosa_chinensis	RchiOBHm_Chr6g0311921
rosa_multiflora	Rmu_ssc0000057.1_g000001
rosa_roxburghii	Rroxscaffold_7G00157220
rosa_rugosa	Rorug06G0391300 Rorug06G0391400 Rorug06G0391400
rosa_samantha	Rh6AG507800 Rh6BG519000 Rh6CG524700 Rh6DG510300
rosa_wichuraiana	Rw6G044200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	2 cut(s)	332, 1211
Acc36I	ACCTGC	1 cut(s)	1702
AccB1I	GGYRCC	1 cut(s)	211
AccB7I	CCANNNNNTGG	1 cut(s)	1865
AccI	GTMKAC	6 cut(s)	336, 1155, 1374, 1828, 2108, 2418
AccII	CGCG	3 cut(s)	209, 301, 410
AccIII	TCCGGA	1 cut(s)	2141
AclWI	GGATC	8 cut(s)	230, 601, 652, 766, 786, 799, 935, 1475
AcoI	YGGCCR	2 cut(s)	715, 1888
AcsI	RAATTY	5 cut(s)	627, 772, 1737, 2198, 2464
AcuI	CTGAAG	2 cut(s)	37, 203
AcyI	GRCGYC	3 cut(s)	204, 212, 2421
AfaI	GTAC	3 cut(s)	1029, 1418, 2520
AfiI	CCNNNNNNNGG	6 cut(s)	462, 1124, 1341, 1865, 2148, 2629
AjnI	CCWGG	7 cut(s)	420, 712, 835, 1102, 1858, 1935, 2424
AjuI	GAANNNNNNNTTGG	8 cut(s)	35, 67, 757, 789, 1848, 1880, 2046, 2078
AloI	GAACNNNNNNTCC	2 cut(s)	842, 874
Alw21I	GWGCWC	4 cut(s)	897, 1543, 1629, 2585
Alw26I	GTCTC	9 cut(s)	274, 301, 359, 503, 714, 826, 1077, 1219, 2139
Alw44I	GTGCAC	1 cut(s)	1539
AlwI	GGATC	8 cut(s)	230, 601, 652, 766, 786, 799, 935, 1475
Aor13HI	TCCGGA	1 cut(s)	2141
AoxI	GGCC	5 cut(s)	386, 715, 1469, 1888, 2574
ApaLI	GTGCAC	1 cut(s)	1539
ApoI	RAATTY	5 cut(s)	627, 772, 1737, 2198, 2464
ArsI	GACNNNNNNTTYG	2 cut(s)	713, 745
Asp700I	GAANNNNTTC	2 cut(s)	1182, 1740
AspA2I	CCTAGG	1 cut(s)	898
AspLEI	GCGC	3 cut(s)	214, 1333, 1986
AspS9I	GGNCC	5 cut(s)	710, 1241, 1364, 1469, 1921
AsuC2I	CCSGG	2 cut(s)	274, 472
AsuHPI	GGTGA	4 cut(s)	163, 526, 550, 753
AvaII	GGWCC	4 cut(s)	710, 1241, 1364, 1921
AvrII	CCTAGG	1 cut(s)	898
BaeGI	GKGCMC	1 cut(s)	1543
BamHI	GGATCC	1 cut(s)	791
BanI	GGYRCC	1 cut(s)	211
BanII	GRGCYC	1 cut(s)	2585
BarI	GAAGNNNNNNTAC	4 cut(s)	1320, 1352, 1699, 1731
Bbv12I	GWGCWC	4 cut(s)	897, 1543, 1629, 2585
BbvCI	CCTCAGC	2 cut(s)	17, 383
BceAI	ACGGC	2 cut(s)	114, 384
BciT130I	CCWGG	7 cut(s)	422, 714, 837, 1104, 1860, 1937, 2426
BcnI	CCSGG	2 cut(s)	274, 472
BcoDI	GTCTC	9 cut(s)	274, 301, 359, 503, 714, 826, 1077, 1219, 2139
BfaI	CTAG	5 cut(s)	899, 939, 1571, 1970, 2247
BfmI	CTRYAG	5 cut(s)	1560, 1755, 1872, 2169, 2615
BfoI	RGCGCY	2 cut(s)	215, 1334
BfuAI	ACCTGC	1 cut(s)	1702
BlnI	CCTAGG	1 cut(s)	898
BlpI	GCTNAGC	1 cut(s)	1636
BmcAI	AGTACT	1 cut(s)	2520
Bme1390I	CCNGG	9 cut(s)	274, 422, 472, 714, 837, 1104, 1860, 1937, 2426
Bme18I	GGWCC	4 cut(s)	710, 1241, 1364, 1921
BmgT120I	GGNCC	5 cut(s)	710, 1241, 1364, 1469, 1921
BmiI	GGNNCC	6 cut(s)	213, 711, 793, 1273, 1922, 1934
BmrFI	CCNGG	9 cut(s)	274, 422, 472, 714, 837, 1104, 1860, 1937, 2426
BmrI	ACTGGG	2 cut(s)	1243, 1553
BmuI	ACTGGG	2 cut(s)	1243, 1553
BplI	GAGNNNNNCTC	2 cut(s)	10, 42
BpmI	CTGGAG	4 cut(s)	58, 173, 1000, 2256
Bpu10I	CCTNAGC	2 cut(s)	17, 383
Bpu1102I	GCTNAGC	1 cut(s)	1636
BpuEI	CTTGAG	4 cut(s)	932, 1302, 2394, 2599
BpuMI	CCSGG	2 cut(s)	274, 472
BsaBI	GATNNNNATC	2 cut(s)	790, 1479
BsaHI	GRCGYC	3 cut(s)	204, 212, 2421
BsaI	GGTCTC	1 cut(s)	826
BsaJI	CCNNGG	6 cut(s)	207, 246, 835, 836, 898, 2424
BsaWI	WCCGGW	2 cut(s)	695, 2141
BsaXI	ACNNNNNCTCC	4 cut(s)	1074, 1104, 1608, 1638
Bsc4I	CCNNNNNNNGG	6 cut(s)	462, 1124, 1341, 1865, 2148, 2629
Bse118I	RCCGGY	1 cut(s)	871
Bse1I	ACTGG	4 cut(s)	75, 1238, 1273, 1548
Bse3DI	GCAATG	4 cut(s)	1484, 2283, 2505, 2606
Bse8I	GATNNNNATC	2 cut(s)	790, 1479
BseAI	TCCGGA	1 cut(s)	2141
BseBI	CCWGG	7 cut(s)	422, 714, 837, 1104, 1860, 1937, 2426
BseDI	CCNNGG	6 cut(s)	207, 246, 835, 836, 898, 2424
BseGI	GGATG	9 cut(s)	282, 480, 856, 1129, 1243, 1318, 1777, 2499, 2574
BseJI	GATNNNNATC	2 cut(s)	790, 1479
BseLI	CCNNNNNNNGG	6 cut(s)	462, 1124, 1341, 1865, 2148, 2629
BseMI	GCAATG	4 cut(s)	1484, 2283, 2505, 2606
BseMII	CTCAG	5 cut(s)	8, 374, 731, 1230, 2094
BseNI	ACTGG	4 cut(s)	75, 1238, 1273, 1548
BseRI	GAGGAG	8 cut(s)	22, 38, 61, 134, 475, 542, 929, 1629
BseSI	GKGCMC	1 cut(s)	1543
BseYI	CCCAGC	1 cut(s)	1466
Bsh1236I	CGCG	3 cut(s)	209, 301, 410
Bsh1285I	CGRYCG	1 cut(s)	331
BshFI	GGCC	5 cut(s)	388, 717, 1471, 1890, 2576
BshNI	GGYRCC	1 cut(s)	211
BsiEI	CGRYCG	1 cut(s)	331
BsiHKAI	GWGCWC	4 cut(s)	897, 1543, 1629, 2585
BsiSI	CCGG	9 cut(s)	273, 389, 471, 696, 872, 1362, 2142, 2514, 2562
BslFI	GGGAC	4 cut(s)	723, 1043, 1591, 1614
BslI	CCNNNNNNNGG	6 cut(s)	462, 1124, 1341, 1865, 2148, 2629
BsmAI	GTCTC	9 cut(s)	274, 301, 359, 503, 714, 826, 1077, 1219, 2139
BsmBI	CGTCTC	3 cut(s)	301, 359, 503
BsmFI	GGGAC	4 cut(s)	723, 1043, 1591, 1614
BsmI	GAATGC	1 cut(s)	616
BsnI	GGCC	5 cut(s)	388, 717, 1471, 1890, 2576
Bso31I	GGTCTC	1 cut(s)	826
Bsp1286I	GDGCHC	4 cut(s)	897, 1543, 1629, 2585
Bsp13I	TCCGGA	1 cut(s)	2141
Bsp1720I	GCTNAGC	1 cut(s)	1636
Bsp68I	TCGCGA	1 cut(s)	301
BspANI	GGCC	5 cut(s)	388, 717, 1471, 1890, 2576
BspCNI	CTCAG	5 cut(s)	9, 375, 732, 1229, 2095
BspEI	TCCGGA	1 cut(s)	2141
BspFNI	CGCG	3 cut(s)	209, 301, 410
BspHI	TCATGA	1 cut(s)	2095
BspLI	GGNNCC	6 cut(s)	213, 711, 793, 1273, 1922, 1934
BspMAI	CTGCAG	1 cut(s)	1759
BspMI	ACCTGC	1 cut(s)	1702
BspPI	GGATC	8 cut(s)	230, 601, 652, 766, 786, 799, 935, 1475
BspQI	GCTCTTC	1 cut(s)	536
BspT107I	GGYRCC	1 cut(s)	211
BspTNI	GGTCTC	1 cut(s)	826
BsrDI	GCAATG	4 cut(s)	1484, 2283, 2505, 2606
BsrFI	RCCGGY	1 cut(s)	871
BsrI	ACTGG	4 cut(s)	75, 1238, 1273, 1548
BssAI	RCCGGY	1 cut(s)	871
BssECI	CCNNGG	6 cut(s)	207, 246, 835, 836, 898, 2424
BssNAI	GTATAC	3 cut(s)	1375, 1829, 2109
BssNI	GRCGYC	3 cut(s)	204, 212, 2421
BssT1I	CCWWGG	1 cut(s)	898
Bst1107I	GTATAC	3 cut(s)	1375, 1829, 2109
Bst2UI	CCWGG	7 cut(s)	422, 714, 837, 1104, 1860, 1937, 2426
Bst6I	CTCTTC	6 cut(s)	56, 536, 831, 1123, 1722, 2148
BstACI	GRCGYC	3 cut(s)	204, 212, 2421
BstC8I	GCNNGC	4 cut(s)	600, 1347, 1642, 2090
BstDEI	CTNAG	6 cut(s)	17, 383, 740, 1216, 1636, 2103
BstDSI	CCRYGG	1 cut(s)	207
BstF5I	GGATG	9 cut(s)	282, 480, 856, 1129, 1243, 1318, 1777, 2499, 2574
BstFNI	CGCG	3 cut(s)	209, 301, 410
BstH2I	RGCGCY	2 cut(s)	215, 1334
BstHHI	GCGC	3 cut(s)	214, 1333, 1986
BstMAI	GTCTC	9 cut(s)	274, 301, 359, 503, 714, 826, 1077, 1219, 2139
BstMCI	CGRYCG	1 cut(s)	331
BstNI	CCWGG	7 cut(s)	422, 714, 837, 1104, 1860, 1937, 2426
BstNSI	RCATGY	1 cut(s)	602
BstSCI	CCNGG	9 cut(s)	272, 420, 470, 712, 835, 1102, 1858, 1935, 2424
BstSFI	CTRYAG	5 cut(s)	1560, 1755, 1872, 2169, 2615
BstSLI	GKGCMC	1 cut(s)	1543
BstUI	CGCG	3 cut(s)	209, 301, 410
BstX2I	RGATCY	2 cut(s)	791, 1480
BstXI	CCANNNNNNTGG	1 cut(s)	1120
BstYI	RGATCY	2 cut(s)	791, 1480
BstZ17I	GTATAC	3 cut(s)	1375, 1829, 2109
BsuRI	GGCC	5 cut(s)	388, 717, 1471, 1890, 2576
BtgI	CCRYGG	1 cut(s)	207
BtgZI	GCGATG	1 cut(s)	2301
BtsCI	GGATG	9 cut(s)	282, 480, 856, 1129, 1243, 1318, 1777, 2499, 2574
BtsI	GCAGTG	1 cut(s)	1764
BtsIMutI	CAGTG	5 cut(s)	82, 962, 1030, 1541, 1764
BtuMI	TCGCGA	1 cut(s)	301
BveI	ACCTGC	1 cut(s)	1702
Cac8I	GCNNGC	4 cut(s)	600, 1347, 1642, 2090
CciI	TCATGA	1 cut(s)	2095
CfoI	GCGC	3 cut(s)	214, 1333, 1986
Cfr10I	RCCGGY	1 cut(s)	871
Cfr13I	GGNCC	5 cut(s)	710, 1241, 1364, 1469, 1921
Cfr42I	CCGCGG	1 cut(s)	210
CseI	GACGC	5 cut(s)	212, 283, 2081, 2309, 2429
CsiI	ACCWGGT	1 cut(s)	1858
Csp6I	GTAC	3 cut(s)	1028, 1417, 2519
CviQI	GTAC	3 cut(s)	1028, 1417, 2519
DdeI	CTNAG	6 cut(s)	17, 383, 740, 1216, 1636, 2103
DinI	GGCGCC	1 cut(s)	213
DrdI	GACNNNNNNGTC	2 cut(s)	332, 1211
DseDI	GACNNNNNNGTC	2 cut(s)	332, 1211
EaeI	YGGCCR	2 cut(s)	715, 1888
Eam1104I	CTCTTC	6 cut(s)	56, 536, 831, 1123, 1722, 2148
EarI	CTCTTC	6 cut(s)	56, 536, 831, 1123, 1722, 2148
EciI	GGCGGA	3 cut(s)	137, 187, 310
Ecl136II	GAGCTC	1 cut(s)	2583
Eco130I	CCWWGG	1 cut(s)	898
Eco24I	GRGCYC	1 cut(s)	2585
Eco31I	GGTCTC	1 cut(s)	826
Eco32I	GATATC	1 cut(s)	1287
Eco47I	GGWCC	4 cut(s)	710, 1241, 1364, 1921
Eco53kI	GAGCTC	1 cut(s)	2583
Eco57I	CTGAAG	2 cut(s)	37, 203
EcoICRI	GAGCTC	1 cut(s)	2583
EcoO109I	RGGNCCY	1 cut(s)	710
EcoRI	GAATTC	2 cut(s)	627, 772
EcoRII	CCWGG	7 cut(s)	420, 712, 835, 1102, 1858, 1935, 2424
EcoRV	GATATC	1 cut(s)	1287
EcoT14I	CCWWGG	1 cut(s)	898
EcoT38I	GRGCYC	1 cut(s)	2585
EgeI	GGCGCC	1 cut(s)	213
EheI	GGCGCC	1 cut(s)	213
ErhI	CCWWGG	1 cut(s)	898
Esp3I	CGTCTC	3 cut(s)	301, 359, 503
FalI	AAGNNNNNCTT	4 cut(s)	35, 67, 969, 1001
FaqI	GGGAC	4 cut(s)	723, 1043, 1591, 1614
FauI	CCCGC	5 cut(s)	250, 418, 676, 1242, 2558
FblI	GTMKAC	6 cut(s)	336, 1155, 1374, 1828, 2108, 2418
FokI	GGATG	9 cut(s)	289, 487, 863, 1136, 1250, 1325, 1764, 2486, 2581
FriOI	GRGCYC	1 cut(s)	2585
FspBI	CTAG	5 cut(s)	899, 939, 1571, 1970, 2247
GlaI	GCGC	3 cut(s)	213, 1332, 1985
GsaI	CCCAGC	1 cut(s)	1470
GsuI	CTGGAG	4 cut(s)	58, 173, 1000, 2256
HaeII	RGCGCY	2 cut(s)	215, 1334
HaeIII	GGCC	5 cut(s)	388, 717, 1471, 1890, 2576
HapII	CCGG	9 cut(s)	273, 389, 471, 696, 872, 1362, 2142, 2514, 2562
HgaI	GACGC	5 cut(s)	212, 283, 2081, 2309, 2429
HhaI	GCGC	3 cut(s)	214, 1333, 1986
Hin1I	GRCGYC	3 cut(s)	204, 212, 2421
Hin6I	GCGC	3 cut(s)	212, 1331, 1984
HinP1I	GCGC	3 cut(s)	212, 1331, 1984
HincII	GTYRAC	4 cut(s)	82, 337, 352, 1156
HindII	GTYRAC	4 cut(s)	82, 337, 352, 1156
HpaII	CCGG	9 cut(s)	273, 389, 471, 696, 872, 1362, 2142, 2514, 2562
HphI	GGTGA	4 cut(s)	163, 526, 550, 753
Hpy99I	CGWCG	4 cut(s)	206, 329, 1003, 1160
HpyAV	CCTTC	9 cut(s)	499, 517, 673, 1679, 1836, 1991, 2019, 2042, 2048
HpyCH4IV	ACGT	3 cut(s)	904, 2271, 2325
HpyF3I	CTNAG	6 cut(s)	17, 383, 740, 1216, 1636, 2103
HpySE526I	ACGT	3 cut(s)	904, 2271, 2325
Hsp92I	GRCGYC	3 cut(s)	204, 212, 2421
HspAI	GCGC	3 cut(s)	212, 1331, 1984
KasI	GGCGCC	1 cut(s)	211
Kpn2I	TCCGGA	1 cut(s)	2141
KspI	CCGCGG	1 cut(s)	210
LguI	GCTCTTC	1 cut(s)	536
MabI	ACCWGGT	1 cut(s)	1858
MaeI	CTAG	5 cut(s)	899, 939, 1571, 1970, 2247
MaeII	ACGT	3 cut(s)	904, 2271, 2325
MaeIII	GTNAC	2 cut(s)	960, 2433
MfeI	CAATTG	1 cut(s)	1782
MflI	RGATCY	2 cut(s)	791, 1480
MhlI	GDGCHC	4 cut(s)	897, 1543, 1629, 2585
Mly113I	GGCGCC	1 cut(s)	212
MlyI	GAGTC	8 cut(s)	312, 332, 342, 1079, 1381, 1596, 1739, 2212
MmeI	TCCRAC	5 cut(s)	159, 465, 981, 1060, 2164
MroI	TCCGGA	1 cut(s)	2141
MroXI	GAANNNNTTC	2 cut(s)	1182, 1740
MseI	TTAA	4 cut(s)	749, 1799, 1850, 2253
MslI	CAYNNNNRTG	1 cut(s)	1118
MspA1I	CMGCKG	2 cut(s)	209, 1220
MspI	CCGG	9 cut(s)	273, 389, 471, 696, 872, 1362, 2142, 2514, 2562
MspR9I	CCNGG	9 cut(s)	274, 422, 472, 714, 837, 1104, 1860, 1937, 2426
MunI	CAATTG	1 cut(s)	1782
Mva1269I	GAATGC	1 cut(s)	616
MvaI	CCWGG	7 cut(s)	422, 714, 837, 1104, 1860, 1937, 2426
MvnI	CGCG	3 cut(s)	209, 301, 410
NarI	GGCGCC	1 cut(s)	212
NciI	CCSGG	2 cut(s)	274, 472
NlaIV	GGNNCC	6 cut(s)	213, 711, 793, 1273, 1922, 1934
NmeAIII	GCCGAG	2 cut(s)	271, 743
NmuCI	GTSAC	2 cut(s)	960, 2433
NruI	TCGCGA	1 cut(s)	301
NspI	RCATGY	1 cut(s)	602
PaeI	GCATGC	1 cut(s)	602
PagI	TCATGA	1 cut(s)	2095
PasI	CCCWGGG	1 cut(s)	836
PciSI	GCTCTTC	1 cut(s)	536
PcsI	WCGNNNNNNNCGW	1 cut(s)	333
PctI	GAATGC	1 cut(s)	616
PdmI	GAANNNNTTC	2 cut(s)	1182, 1740
PfeI	GAWTC	6 cut(s)	447, 786, 1010, 1160, 1178, 1352
PflFI	GACNNNGTC	1 cut(s)	332
PflMI	CCANNNNNTGG	1 cut(s)	1865
PfoI	TCCNGGA	2 cut(s)	272, 470
PleI	GAGTC	8 cut(s)	311, 332, 341, 1079, 1381, 1596, 1738, 2212
PluTI	GGCGCC	1 cut(s)	215
PpsI	GAGTC	8 cut(s)	311, 332, 341, 1079, 1381, 1596, 1738, 2212
PpuMI	RGGWCCY	1 cut(s)	710
Psp124BI	GAGCTC	1 cut(s)	2585
Psp5II	RGGWCCY	1 cut(s)	710
Psp6I	CCWGG	7 cut(s)	420, 712, 835, 1102, 1858, 1935, 2424
PspFI	CCCAGC	1 cut(s)	1466
PspGI	CCWGG	7 cut(s)	420, 712, 835, 1102, 1858, 1935, 2424
PspN4I	GGNNCC	6 cut(s)	213, 711, 793, 1273, 1922, 1934
PspPI	GGNCC	5 cut(s)	710, 1241, 1364, 1469, 1921
PspPPI	RGGWCCY	1 cut(s)	710
PstI	CTGCAG	1 cut(s)	1759
PsuI	RGATCY	2 cut(s)	791, 1480
PsyI	GACNNNGTC	1 cut(s)	332
PvuII	CAGCTG	1 cut(s)	1220
RruI	TCGCGA	1 cut(s)	301
RsaI	GTAC	3 cut(s)	1029, 1418, 2520
RsaNI	GTAC	3 cut(s)	1028, 1417, 2519
RseI	CAYNNNNRTG	1 cut(s)	1118
SacI	GAGCTC	1 cut(s)	2585
SacII	CCGCGG	1 cut(s)	210
SalI	GTCGAC	2 cut(s)	335, 1154
SapI	GCTCTTC	1 cut(s)	536
SaqAI	TTAA	4 cut(s)	749, 1799, 1850, 2253
Sau96I	GGNCC	5 cut(s)	710, 1241, 1364, 1469, 1921
ScaI	AGTACT	1 cut(s)	2520
SchI	GAGTC	8 cut(s)	312, 332, 342, 1079, 1381, 1596, 1739, 2212
ScrFI	CCNGG	9 cut(s)	274, 422, 472, 714, 837, 1104, 1860, 1937, 2426
SduI	GDGCHC	4 cut(s)	897, 1543, 1629, 2585
SexAI	ACCWGGT	1 cut(s)	1858
SfcI	CTRYAG	5 cut(s)	1560, 1755, 1872, 2169, 2615
SfoI	GGCGCC	1 cut(s)	213
Sfr303I	CCGCGG	1 cut(s)	210
SgrBI	CCGCGG	1 cut(s)	210
SinI	GGWCC	4 cut(s)	710, 1241, 1364, 1921
SmiMI	CAYNNNNRTG	1 cut(s)	1118
SmlI	CTYRAG	4 cut(s)	911, 1317, 2409, 2578
SmoI	CTYRAG	4 cut(s)	911, 1317, 2409, 2578
SphI	GCATGC	1 cut(s)	602
SspDI	GGCGCC	1 cut(s)	211
SspI	AATATT	1 cut(s)	2589
SspMI	CTAG	5 cut(s)	899, 939, 1571, 1970, 2247
SstI	GAGCTC	1 cut(s)	2585
StyD4I	CCNGG	9 cut(s)	272, 420, 470, 712, 835, 1102, 1858, 1935, 2424
StyI	CCWWGG	1 cut(s)	898
TaiI	ACGT	3 cut(s)	907, 2274, 2328
TaqI	TCGA	6 cut(s)	264, 336, 864, 1075, 1155, 1629
TaqII	GACCGA	2 cut(s)	345, 377
TatI	WGTACW	2 cut(s)	1027, 2518
TfiI	GAWTC	6 cut(s)	447, 786, 1010, 1160, 1178, 1352
Tru1I	TTAA	4 cut(s)	749, 1799, 1850, 2253
Tru9I	TTAA	4 cut(s)	749, 1799, 1850, 2253
TscAI	CASTG	5 cut(s)	82, 969, 1030, 1548, 1764
TseFI	GTSAC	2 cut(s)	960, 2433
Tsp45I	GTSAC	2 cut(s)	960, 2433
TspDTI	ATGAA	8 cut(s)	620, 785, 962, 966, 1341, 1740, 2211, 2595
TspGWI	ACGGA	1 cut(s)	452
TspRI	CASTG	5 cut(s)	82, 969, 1030, 1548, 1764
Tth111I	GACNNNGTC	1 cut(s)	332
Van91I	CCANNNNNTGG	1 cut(s)	1865
VneI	GTGCAC	1 cut(s)	1539
VpaK11BI	GGWCC	4 cut(s)	710, 1241, 1364, 1921
XapI	RAATTY	5 cut(s)	627, 772, 1737, 2198, 2464
XbaI	TCTAGA	1 cut(s)	1969
XceI	RCATGY	1 cut(s)	602
XmaJI	CCTAGG	1 cut(s)	898
XmiI	GTMKAC	6 cut(s)	336, 1155, 1374, 1828, 2108, 2418
XmnI	GAANNNNTTC	2 cut(s)	1182, 1740
XspI	CTAG	5 cut(s)	899, 939, 1571, 1970, 2247
ZrmI	AGTACT	1 cut(s)	2520

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.