pycom15g05080
ERF Family

Belongs to the class I-like SAM-binding methyltransferase superfamily. RsmB NOP family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0009262
Physical Location & Seq
Reverse (-)
3050829 .. 3052857
2029 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 846 bp
ATGCAAGGAAAGGCAAGCTCTATGGTGGCTGCAGCCCTTGCTCCGAAACCAGGATGGGAGGTTCTTGATGCATGCTCGGCTCCTGGAAACAAAACTGTGCACCTTGCAGCTCTTATGAGAGGAGAGGGAAAAGTAATAGCATGTGAACTGAATGAAGACAGGGTTAAACGTTTAAAAGAGACTATCAGACTTTCTGGTGCTTCTAATATTAAGGTTGTGCATGGAGATTTCTTAGACTTAAACCCTGAAGATCCTCAATTTTCCAAGGTCCGGGCCATTCTTTTAGATCCTTCCTGCTCTGGATCTGGGACTGCTTCTGCAAGATTAGATCATTTGCTCCCATCTTCTGCTCCAGGTCAGGGTACCGATTTTGCCAGCACAGAAAGACTTAACAAGCTCGCTGCCTTTCAGAGAAAAGCTCTGGCTCATGCTTTATCTTTTCCGGAAGTGGAGAGAGTTGTTTACAGCACATGCTCTGTTAACCAAGTCGAAAACGAAGATGTCGTTAAATCTGTGCTACCACTTGCTGCATCCAATGGTTTCCAACTGGAAACCCCCTTCCCGAAATGGCACCGCCGTGGTCTCCCCGTTATCGAGGGCGCCGAATGCTTGATTAGGACCGATCCGAAGGAGGACAAAGAAGGCTTCTTCATCGCTCTGTTTTCCAGGAGCTCCAGCACCCAACAAGAGGCGCCTAATGGAACCGGCAGAGACGCCACCGAAGCCTCGAAGAGGCAGAGAAATTATGCAGTGCCTCATCTCAATCTGTTTAAAACGTGGACACATGCGCAGCGGATGTGGCGTAATAATTCTAGCCACACTCACAGTCAATGTCATTGTTGCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Methyltr_RsmB-F

Protein Analysis

282

Amino Acids

30.74

Weight (kDa)

8.61

Isoelectric Point (pI)

41.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ubie_methyltran PF01209 9 - 88 2.7e-06 ubiE/COQ5 methyltransferase family
Methyltr_RsmB-F PF01189 11 - 221 1.8e-26 16S rRNA methyltransferase RsmB/F
Methyltransf_31 PF13847 16 - 84 3.7e-06 Methyltransferase domain
Methyltransf_25 PF13649 21 - 92 2.7e-07 Methyltransferase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 789
Acc65I GGTACC 1 cut(s) 362
AccB1I GGYRCC 4 cut(s) 362, 570, 599, 691
AccIII TCCGGA 1 cut(s) 442
AciI CCGC 2 cut(s) 574, 793
AclI AACGTT 1 cut(s) 169
AclWI GGATC 4 cut(s) 245, 281, 310, 617
AcuI CTGAAG 1 cut(s) 267
AcyI GRCGYC 3 cut(s) 600, 692, 714
AfaI GTAC 1 cut(s) 364
AfiI CCNNNNNNNGG 5 cut(s) 50, 270, 359, 688, 732
AjnI CCWGG 4 cut(s) 49, 82, 352, 665
AleI CACNNNNGTG 1 cut(s) 576
AloI GAACNNNNNNTCC 2 cut(s) 45, 77
AluBI AGCT 5 cut(s) 18, 110, 397, 419, 672
AluI AGCT 5 cut(s) 18, 110, 397, 419, 672
Alw21I GWGCWC 2 cut(s) 102, 674
Alw26I GTCTC 3 cut(s) 173, 587, 705
Alw44I GTGCAC 1 cut(s) 98
AlwI GGATC 4 cut(s) 245, 281, 310, 617
Aor13HI TCCGGA 1 cut(s) 442
AoxI GGCC 1 cut(s) 273
ApaLI GTGCAC 1 cut(s) 98
ApeKI GCWGC 6 cut(s) 29, 32, 107, 401, 527, 790
Asp718I GGTACC 1 cut(s) 362
AspLEI GCGC 3 cut(s) 602, 694, 790
AspS9I GGNCC 3 cut(s) 268, 273, 618
AsuC2I CCSGG 1 cut(s) 272
AvaII GGWCC 2 cut(s) 268, 618
BaeGI GKGCMC 1 cut(s) 102
BanI GGYRCC 4 cut(s) 362, 570, 599, 691
BanII GRGCYC 1 cut(s) 674
BbsI GAAGAC 1 cut(s) 162
Bbv12I GWGCWC 2 cut(s) 102, 674
BbvI GCAGC 6 cut(s) 16, 44, 119, 388, 514, 802
BccI CCATC 2 cut(s) 48, 349
BceAI ACGGC 1 cut(s) 561
BciT130I CCWGG 4 cut(s) 51, 84, 354, 667
BcnI CCSGG 1 cut(s) 272
BcoDI GTCTC 3 cut(s) 173, 587, 705
BfaI CTAG 1 cut(s) 813
BfmI CTRYAG 1 cut(s) 30
BfoI RGCGCY 2 cut(s) 603, 695
BisI GCNGC 6 cut(s) 30, 33, 108, 402, 528, 791
BlsI GCNGC 6 cut(s) 31, 34, 109, 403, 529, 792
Bme1390I CCNGG 5 cut(s) 51, 84, 272, 354, 667
Bme18I GGWCC 2 cut(s) 268, 618
BmgT120I GGNCC 3 cut(s) 268, 273, 618
BmiI GGNNCC 6 cut(s) 81, 364, 572, 601, 693, 703
BmrFI CCNGG 5 cut(s) 51, 84, 272, 354, 667
BmsI GCATC 2 cut(s) 58, 539
BpiI GAAGAC 1 cut(s) 162
BplI GAGNNNNNCTC 2 cut(s) 403, 435
BpmI CTGGAG 2 cut(s) 336, 658
BpuMI CCSGG 1 cut(s) 272
BsaHI GRCGYC 3 cut(s) 600, 692, 714
BsaI GGTCTC 1 cut(s) 587
BsaJI CCNNGG 2 cut(s) 264, 577
BsaWI WCCGGW 1 cut(s) 442
Bsc4I CCNNNNNNNGG 5 cut(s) 50, 270, 359, 688, 732
Bse118I RCCGGY 1 cut(s) 704
Bse1I ACTGG 1 cut(s) 552
BseAI TCCGGA 1 cut(s) 442
BseBI CCWGG 4 cut(s) 51, 84, 354, 667
BseDI CCNNGG 2 cut(s) 264, 577
BseGI GGATG 3 cut(s) 59, 530, 801
BseLI CCNNNNNNNGG 5 cut(s) 50, 270, 359, 688, 732
BseNI ACTGG 1 cut(s) 552
BseRI GAGGAG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 102
BseXI GCAGC 6 cut(s) 16, 44, 119, 388, 514, 802
BshFI GGCC 1 cut(s) 275
BshNI GGYRCC 4 cut(s) 362, 570, 599, 691
BsiHKAI GWGCWC 2 cut(s) 102, 674
BsiSI CCGG 3 cut(s) 271, 443, 705
BslFI GGGAC 1 cut(s) 322
BslI CCNNNNNNNGG 5 cut(s) 50, 270, 359, 688, 732
BsmAI GTCTC 3 cut(s) 173, 587, 705
BsmBI CGTCTC 1 cut(s) 705
BsmFI GGGAC 1 cut(s) 322
BsmI GAATGC 1 cut(s) 611
BsnI GGCC 1 cut(s) 275
Bso31I GGTCTC 1 cut(s) 587
Bsp1286I GDGCHC 2 cut(s) 102, 674
Bsp13I TCCGGA 1 cut(s) 442
Bsp143I GATC 5 cut(s) 250, 286, 302, 328, 622
BspACI CCGC 2 cut(s) 574, 793
BspANI GGCC 1 cut(s) 275
BspEI TCCGGA 1 cut(s) 442
BspLI GGNNCC 6 cut(s) 81, 364, 572, 601, 693, 703
BspMAI CTGCAG 1 cut(s) 34
BspPI GGATC 4 cut(s) 245, 281, 310, 617
BspT107I GGYRCC 4 cut(s) 362, 570, 599, 691
BspTNI GGTCTC 1 cut(s) 587
BsrFI RCCGGY 1 cut(s) 704
BsrI ACTGG 1 cut(s) 552
BssAI RCCGGY 1 cut(s) 704
BssECI CCNNGG 2 cut(s) 264, 577
BssMI GATC 5 cut(s) 250, 286, 302, 328, 622
BssNI GRCGYC 3 cut(s) 600, 692, 714
BssT1I CCWWGG 1 cut(s) 264
Bst2UI CCWGG 4 cut(s) 51, 84, 354, 667
Bst4CI ACNGT 2 cut(s) 97, 827
Bst6I CTCTTC 1 cut(s) 725
BstACI GRCGYC 3 cut(s) 600, 692, 714
BstAPI GCANNNNNTGC 1 cut(s) 38
BstC8I GCNNGC 4 cut(s) 16, 73, 376, 399
BstDEI CTNAG 1 cut(s) 232
BstDSI CCRYGG 1 cut(s) 577
BstENI CCTNNNNNAGG 1 cut(s) 730
BstF5I GGATG 3 cut(s) 59, 530, 801
BstH2I RGCGCY 2 cut(s) 603, 695
BstHHI GCGC 3 cut(s) 602, 694, 790
BstKTI GATC 5 cut(s) 253, 289, 305, 331, 625
BstMAI GTCTC 3 cut(s) 173, 587, 705
BstMBI GATC 5 cut(s) 250, 286, 302, 328, 622
BstMWI GCNNNNNNNGC 5 cut(s) 38, 77, 606, 722, 799
BstNI CCWGG 4 cut(s) 51, 84, 354, 667
BstNSI RCATGY 4 cut(s) 75, 144, 474, 788
BstSCI CCNGG 5 cut(s) 49, 82, 270, 352, 665
BstSFI CTRYAG 1 cut(s) 30
BstSLI GKGCMC 1 cut(s) 102
BstV1I GCAGC 6 cut(s) 16, 44, 119, 388, 514, 802
BstV2I GAAGAC 1 cut(s) 162
BstX2I RGATCY 3 cut(s) 250, 286, 302
BstYI RGATCY 3 cut(s) 250, 286, 302
BsuRI GGCC 1 cut(s) 275
BtgI CCRYGG 1 cut(s) 577
BtgZI GCGATG 1 cut(s) 637
BtsCI GGATG 3 cut(s) 59, 530, 801
BtsI GCAGTG 1 cut(s) 756
BtsIMutI CAGTG 1 cut(s) 756
Cac8I GCNNGC 4 cut(s) 16, 73, 376, 399
CfoI GCGC 3 cut(s) 602, 694, 790
Cfr10I RCCGGY 1 cut(s) 704
Cfr13I GGNCC 3 cut(s) 268, 273, 618
CseI GACGC 1 cut(s) 722
Csp6I GTAC 1 cut(s) 363
CviAII CATG 6 cut(s) 72, 141, 221, 428, 471, 785
CviQI GTAC 1 cut(s) 363
DdeI CTNAG 1 cut(s) 232
DinI GGCGCC 2 cut(s) 601, 693
DpnI GATC 5 cut(s) 252, 288, 304, 330, 624
DpnII GATC 5 cut(s) 250, 286, 302, 328, 622
DraI TTTAAA 2 cut(s) 174, 772
Eam1104I CTCTTC 1 cut(s) 725
EarI CTCTTC 1 cut(s) 725
Ecl136II GAGCTC 1 cut(s) 672
Eco130I CCWWGG 1 cut(s) 264
Eco24I GRGCYC 1 cut(s) 674
Eco31I GGTCTC 1 cut(s) 587
Eco47I GGWCC 2 cut(s) 268, 618
Eco53kI GAGCTC 1 cut(s) 672
Eco57I CTGAAG 1 cut(s) 267
EcoICRI GAGCTC 1 cut(s) 672
EcoNI CCTNNNNNAGG 1 cut(s) 730
EcoRII CCWGG 4 cut(s) 49, 82, 352, 665
EcoT14I CCWWGG 1 cut(s) 264
EcoT22I ATGCAT 1 cut(s) 73
EcoT38I GRGCYC 1 cut(s) 674
EgeI GGCGCC 2 cut(s) 601, 693
EheI GGCGCC 2 cut(s) 601, 693
ErhI CCWWGG 1 cut(s) 264
Esp3I CGTCTC 1 cut(s) 705
FaeI CATG 6 cut(s) 75, 144, 224, 431, 474, 788
FaiI YATR 9 cut(s) 23, 73, 116, 142, 222, 429, 472, 747, 786
FaqI GGGAC 1 cut(s) 322
FatI CATG 6 cut(s) 71, 140, 220, 427, 470, 784
Fnu4HI GCNGC 6 cut(s) 30, 33, 108, 402, 528, 791
FokI GGATG 3 cut(s) 66, 517, 808
FriOI GRGCYC 1 cut(s) 674
Fsp4HI GCNGC 6 cut(s) 30, 33, 108, 402, 528, 791
FspBI CTAG 1 cut(s) 813
FspI TGCGCA 1 cut(s) 789
GlaI GCGC 3 cut(s) 601, 693, 789
GluI GCNGC 6 cut(s) 30, 33, 108, 402, 528, 791
GsuI CTGGAG 2 cut(s) 336, 658
HaeII RGCGCY 2 cut(s) 603, 695
HaeIII GGCC 1 cut(s) 275
HapII CCGG 3 cut(s) 271, 443, 705
HgaI GACGC 1 cut(s) 722
HhaI GCGC 3 cut(s) 602, 694, 790
Hin1I GRCGYC 3 cut(s) 600, 692, 714
Hin1II CATG 6 cut(s) 75, 144, 224, 431, 474, 788
Hin6I GCGC 3 cut(s) 600, 692, 788
HinP1I GCGC 3 cut(s) 600, 692, 788
HincII GTYRAC 1 cut(s) 481
HindII GTYRAC 1 cut(s) 481
HpaI GTTAAC 1 cut(s) 481
HpaII CCGG 3 cut(s) 271, 443, 705
Hpy166II GTNNAC 5 cut(s) 100, 146, 463, 481, 780
Hpy188I TCNGA 4 cut(s) 45, 188, 411, 627
Hpy188III TCNNGA 4 cut(s) 65, 300, 443, 562
Hpy8I GTNNAC 5 cut(s) 100, 146, 463, 481, 780
HpyAV CCTTC 4 cut(s) 300, 568, 622, 635
HpyCH4III ACNGT 2 cut(s) 97, 827
HpyCH4IV ACGT 2 cut(s) 169, 776
HpyCH4V TGCA 9 cut(s) 4, 32, 71, 100, 107, 220, 320, 530, 749
HpyF10VI GCNNNNNNNGC 5 cut(s) 38, 77, 606, 722, 799
HpyF3I CTNAG 1 cut(s) 232
HpySE526I ACGT 2 cut(s) 169, 776
Hsp92I GRCGYC 3 cut(s) 600, 692, 714
Hsp92II CATG 6 cut(s) 75, 144, 224, 431, 474, 788
HspAI GCGC 3 cut(s) 600, 692, 788
KasI GGCGCC 2 cut(s) 599, 691
Kpn2I TCCGGA 1 cut(s) 442
KpnI GGTACC 1 cut(s) 366
KspAI GTTAAC 1 cut(s) 481
Kzo9I GATC 5 cut(s) 250, 286, 302, 328, 622
LmnI GCTCC 6 cut(s) 46, 85, 342, 355, 669, 677
Lsp1109I GCAGC 6 cut(s) 16, 44, 119, 388, 514, 802
LweI GCATC 2 cut(s) 58, 539
MaeI CTAG 1 cut(s) 813
MaeII ACGT 2 cut(s) 169, 776
MalI GATC 5 cut(s) 252, 288, 304, 330, 624
MboI GATC 5 cut(s) 250, 286, 302, 328, 622
MboII GAAGA 6 cut(s) 167, 260, 336, 509, 640, 742
MflI RGATCY 3 cut(s) 250, 286, 302
MhlI GDGCHC 2 cut(s) 102, 674
MluCI AATT 3 cut(s) 257, 742, 808
Mly113I GGCGCC 2 cut(s) 600, 692
MmeI TCCRAC 1 cut(s) 568
Mph1103I ATGCAT 1 cut(s) 73
MroI TCCGGA 1 cut(s) 442
MseI TTAA 8 cut(s) 165, 173, 210, 239, 390, 480, 507, 771
MslI CAYNNNNRTG 1 cut(s) 576
MspA1I CMGCKG 1 cut(s) 793
MspI CCGG 3 cut(s) 271, 443, 705
MspR9I CCNGG 5 cut(s) 51, 84, 272, 354, 667
Mva1269I GAATGC 1 cut(s) 611
MvaI CCWGG 4 cut(s) 51, 84, 354, 667
MwoI GCNNNNNNNGC 5 cut(s) 38, 77, 606, 722, 799
NarI GGCGCC 2 cut(s) 600, 692
NciI CCSGG 1 cut(s) 272
NdeII GATC 5 cut(s) 250, 286, 302, 328, 622
NlaIII CATG 6 cut(s) 75, 144, 224, 431, 474, 788
NlaIV GGNNCC 6 cut(s) 81, 364, 572, 601, 693, 703
NmeAIII GCCGAG 1 cut(s) 56
NsbI TGCGCA 1 cut(s) 789
NsiI ATGCAT 1 cut(s) 73
NspI RCATGY 4 cut(s) 75, 144, 474, 788
OliI CACNNNNGTG 1 cut(s) 576
PaeI GCATGC 1 cut(s) 75
PcsI WCGNNNNNNNCGW 2 cut(s) 501, 600
PctI GAATGC 1 cut(s) 611
PfoI TCCNGGA 2 cut(s) 82, 665
PkrI GCNGC 6 cut(s) 31, 34, 109, 403, 529, 792
PluTI GGCGCC 2 cut(s) 603, 695
Psp124BI GAGCTC 1 cut(s) 674
Psp1406I AACGTT 1 cut(s) 169
Psp6I CCWGG 4 cut(s) 49, 82, 352, 665
PspGI CCWGG 4 cut(s) 49, 82, 352, 665
PspN4I GGNNCC 6 cut(s) 81, 364, 572, 601, 693, 703
PspPI GGNCC 3 cut(s) 268, 273, 618
PstI CTGCAG 1 cut(s) 34
PsuI RGATCY 3 cut(s) 250, 286, 302
RsaI GTAC 1 cut(s) 364
RsaNI GTAC 1 cut(s) 363
RseI CAYNNNNRTG 1 cut(s) 576
SacI GAGCTC 1 cut(s) 674
SaqAI TTAA 8 cut(s) 165, 173, 210, 239, 390, 480, 507, 771
SatI GCNGC 6 cut(s) 30, 33, 108, 402, 528, 791
Sau3AI GATC 5 cut(s) 250, 286, 302, 328, 622
Sau96I GGNCC 3 cut(s) 268, 273, 618
ScrFI CCNGG 5 cut(s) 51, 84, 272, 354, 667
SduI GDGCHC 2 cut(s) 102, 674
SfaNI GCATC 2 cut(s) 58, 539
SfcI CTRYAG 1 cut(s) 30
SfoI GGCGCC 2 cut(s) 601, 693
SinI GGWCC 2 cut(s) 268, 618
SmiMI CAYNNNNRTG 1 cut(s) 576
SphI GCATGC 1 cut(s) 75
Sse9I AATT 3 cut(s) 257, 742, 808
SsiI CCGC 2 cut(s) 574, 793
SspDI GGCGCC 2 cut(s) 599, 691
SspI AATATT 1 cut(s) 208
SspMI CTAG 1 cut(s) 813
SstI GAGCTC 1 cut(s) 674
StyD4I CCNGG 5 cut(s) 49, 82, 270, 352, 665
StyI CCWWGG 1 cut(s) 264
TaaI ACNGT 2 cut(s) 97, 827
TaiI ACGT 2 cut(s) 172, 779
TaqI TCGA 3 cut(s) 489, 594, 728
TaqII GACCGA 1 cut(s) 635
TasI AATT 3 cut(s) 257, 742, 808
Tru1I TTAA 8 cut(s) 165, 173, 210, 239, 390, 480, 507, 771
Tru9I TTAA 8 cut(s) 165, 173, 210, 239, 390, 480, 507, 771
TscAI CASTG 1 cut(s) 756
TseI GCWGC 6 cut(s) 29, 32, 107, 401, 527, 790
TspDTI ATGAA 2 cut(s) 168, 640
TspRI CASTG 1 cut(s) 756
VneI GTGCAC 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 268, 618
XagI CCTNNNNNAGG 1 cut(s) 730
XceI RCATGY 4 cut(s) 75, 144, 474, 788
XspI CTAG 1 cut(s) 813
Zsp2I ATGCAT 1 cut(s) 73
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.