pycom15g07170

isoaspartyl peptidase L-asparaginase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0008520
Physical Location & Seq
Reverse (-)
4383389 .. 4385904
2516 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 978 bp
ATGGGTGGTTGGGCAATTGCAGTTCACGGCGGCGCGGGTGTCGACCCGAATCTCCCCATGGAGCGTCAAGAGCAGGCCAAACAACTCCTCACCCGCTGCCTCAATCTCGGCATCTCTGCTCTCCGCTCCGATCTAGCCGCCATTGATGTTGTCGAGCTTGTCGTTAGGGAATTGGAAACCGATCCTTTCTTCAACTCTGGCCGTGGATCAGCTCTCACAGCGAAAGGGACGGTTGAGATGGAAGCCAGCATCATGGATGGACCCAAAAGACGATGCGGCGCCGTTTCGGGATTAACTACGGTTAAGAATCCCGTTTCACTCGCCCGCCTCGTCATGGACAAATCACCTCACTCCTACCTCGCCTTCTCCGGCGCCGAAGACTTCGCGCGACAACAGGGCGTTGAGCTCGTGGACAATGAGTATTTCATCACAGAGGAAAATGTGGGGATGCTCAAATTGGCAAAGGAGGCCAACACAATCTTGTTTGATTACAGAATTCCGTTAGGATCGGAGAGCTGCAGTGCAGGTGTGGAGAGTCCGTTGCACATGAACGGGCTCCCCATTAGCGTTTACGCACCCGAGACCGTTGGTTGTGTGGTGGTGGATAACCAAGGGCGTTGCGCTGCAGCTACATCCACCGGTGGGCTAATGAACAAAATGACTGGCCGAATCGGTGACTCGCCACTCATTGGTGCCGGTACCTACGCGTGTGACCTTTCTGGGGTCTCGTGCACGGGTGAGGGCGAGGCAATCATACGCGGGACACTCGCGCGTGAGGTGGCAGCAGTGATGGAGTACAAGGGCCTAGGCCTACAGGATGCAGTGGATTTTGTGATCAAAGAGAGGCTTGATGATGGCAAGGCCGGACTCATAGCTGTGTCTAGCAAAGGTGAAGTAGCTTATGGGTTTAATTGTATGGGGATGTTTAGGGGGTGTGCCACTGAGGATGGGTTCATGGAGGTTGGGATTTGGGAGTAG

Protein Analysis

326

Amino Acids

34.32

Weight (kDa)

4.78

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Asparaginase_2 PF01112 4 - 323 5e-100 Asparaginase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 515
Acc36I ACCTGC 1 cut(s) 515
Acc65I GGTACC 1 cut(s) 698
AccB1I GGYRCC 4 cut(s) 278, 371, 692, 698
AccB7I CCANNNNNTGG 1 cut(s) 689
AccBSI CCGCTC 1 cut(s) 126
AccI GTMKAC 1 cut(s) 42
AccII CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
AciI CCGC 8 cut(s) 30, 35, 94, 124, 138, 276, 325, 759
AclWI GGATC 3 cut(s) 176, 214, 514
AcoI YGGCCR 2 cut(s) 199, 664
AcsI RAATTY 1 cut(s) 495
AcyI GRCGYC 2 cut(s) 279, 372
AfaI GTAC 2 cut(s) 700, 797
AfiI CCNNNNNNNGG 4 cut(s) 334, 642, 689, 721
AflIII ACRYGT 1 cut(s) 705
AgeI ACCGGT 1 cut(s) 638
AgsI TTSAA 1 cut(s) 193
AluBI AGCT 7 cut(s) 157, 212, 406, 516, 629, 875, 899
AluI AGCT 7 cut(s) 157, 212, 406, 516, 629, 875, 899
Alw21I GWGCWC 2 cut(s) 408, 734
Alw26I GTCTC 2 cut(s) 575, 730
Alw44I GTGCAC 1 cut(s) 730
AlwI GGATC 3 cut(s) 176, 214, 514
Ama87I CYCGRG 1 cut(s) 578
AoxI GGCC 7 cut(s) 75, 199, 468, 664, 802, 808, 861
ApaLI GTGCAC 1 cut(s) 730
ApeKI GCWGC 5 cut(s) 96, 516, 623, 626, 782
ApoI RAATTY 1 cut(s) 495
AsiGI ACCGGT 1 cut(s) 638
Asp718I GGTACC 1 cut(s) 698
AspA2I CCTAGG 1 cut(s) 805
AspLEI GCGC 6 cut(s) 35, 281, 374, 388, 623, 772
AspS9I GGNCC 2 cut(s) 260, 802
AsuHPI GGTGA 5 cut(s) 82, 336, 686, 749, 902
AvaI CYCGRG 1 cut(s) 578
AvaII GGWCC 1 cut(s) 260
AvrII CCTAGG 1 cut(s) 805
BaeGI GKGCMC 1 cut(s) 734
BanI GGYRCC 4 cut(s) 278, 371, 692, 698
BanII GRGCYC 2 cut(s) 408, 558
BauI CACGAG 2 cut(s) 407, 727
BbsI GAAGAC 1 cut(s) 384
Bbv12I GWGCWC 2 cut(s) 408, 734
BbvI GCAGC 5 cut(s) 83, 503, 610, 638, 794
BccI CCATC 5 cut(s) 232, 251, 784, 848, 941
BceAI ACGGC 3 cut(s) 43, 186, 266
BclI TGATCA 1 cut(s) 834
BcoDI GTCTC 2 cut(s) 575, 730
BfaI CTAG 3 cut(s) 134, 806, 882
BfmI CTRYAG 3 cut(s) 517, 624, 812
BfoI RGCGCY 2 cut(s) 282, 375
BfuAI ACCTGC 1 cut(s) 515
BisI GCNGC 8 cut(s) 31, 97, 138, 277, 517, 624, 627, 783
BlnI CCTAGG 1 cut(s) 805
BlsI GCNGC 8 cut(s) 32, 98, 139, 278, 518, 625, 628, 784
Bme18I GGWCC 1 cut(s) 260
BmeT110I CYCGRG 1 cut(s) 578
BmgT120I GGNCC 2 cut(s) 260, 802
BmiI GGNNCC 6 cut(s) 262, 280, 373, 557, 694, 700
BmsI GCATC 5 cut(s) 120, 258, 263, 438, 808
BpiI GAAGAC 1 cut(s) 384
BsaHI GRCGYC 2 cut(s) 279, 372
BsaI GGTCTC 2 cut(s) 575, 730
BsaJI CCNNGG 4 cut(s) 57, 202, 610, 805
BsaWI WCCGGW 1 cut(s) 638
BsaXI ACNNNNNCTCC 2 cut(s) 524, 554
Bsc4I CCNNNNNNNGG 4 cut(s) 334, 642, 689, 721
Bse118I RCCGGY 2 cut(s) 638, 695
Bse1I ACTGG 1 cut(s) 667
BseDI CCNNGG 4 cut(s) 57, 202, 610, 805
BseGI GGATG 6 cut(s) 262, 453, 632, 823, 927, 952
BseLI CCNNNNNNNGG 4 cut(s) 334, 642, 689, 721
BseMII CTCAG 1 cut(s) 933
BseNI ACTGG 1 cut(s) 667
BseRI GAGGAG 1 cut(s) 77
BseSI GKGCMC 1 cut(s) 734
BseXI GCAGC 5 cut(s) 83, 503, 610, 638, 794
BsgI GTGCAG 1 cut(s) 543
Bsh1236I CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
BshFI GGCC 7 cut(s) 77, 201, 470, 666, 804, 810, 863
BshNI GGYRCC 4 cut(s) 278, 371, 692, 698
BshTI ACCGGT 1 cut(s) 638
BsiHKAI GWGCWC 2 cut(s) 408, 734
BsiHKCI CYCGRG 1 cut(s) 578
BsiSI CCGG 4 cut(s) 369, 639, 696, 864
BslFI GGGAC 2 cut(s) 241, 775
BslI CCNNNNNNNGG 4 cut(s) 334, 642, 689, 721
BsmAI GTCTC 2 cut(s) 575, 730
BsmFI GGGAC 2 cut(s) 241, 775
BsnI GGCC 7 cut(s) 77, 201, 470, 666, 804, 810, 863
Bso31I GGTCTC 2 cut(s) 575, 730
BsoBI CYCGRG 1 cut(s) 578
Bsp1286I GDGCHC 3 cut(s) 408, 558, 734
Bsp143I GATC 5 cut(s) 130, 181, 206, 506, 834
Bsp19I CCATGG 1 cut(s) 57
BspACI CCGC 8 cut(s) 30, 35, 94, 124, 138, 276, 325, 759
BspANI GGCC 7 cut(s) 77, 201, 470, 666, 804, 810, 863
BspCNI CTCAG 1 cut(s) 934
BspFNI CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
BspLI GGNNCC 6 cut(s) 262, 280, 373, 557, 694, 700
BspMAI CTGCAG 2 cut(s) 521, 628
BspMI ACCTGC 1 cut(s) 515
BspPI GGATC 3 cut(s) 176, 214, 514
BspT107I GGYRCC 4 cut(s) 278, 371, 692, 698
BspTNI GGTCTC 2 cut(s) 575, 730
BsrBI CCGCTC 1 cut(s) 126
BsrFI RCCGGY 2 cut(s) 638, 695
BsrI ACTGG 1 cut(s) 667
BssAI RCCGGY 2 cut(s) 638, 695
BssECI CCNNGG 4 cut(s) 57, 202, 610, 805
BssMI GATC 5 cut(s) 130, 181, 206, 506, 834
BssNI GRCGYC 2 cut(s) 279, 372
BssSI CACGAG 2 cut(s) 407, 727
BssT1I CCWWGG 3 cut(s) 57, 610, 805
Bst2BI CACGAG 2 cut(s) 407, 727
Bst4CI ACNGT 3 cut(s) 232, 301, 586
BstACI GRCGYC 2 cut(s) 279, 372
BstC8I GCNNGC 3 cut(s) 75, 247, 325
BstDEI CTNAG 1 cut(s) 942
BstDSI CCRYGG 2 cut(s) 57, 202
BstF5I GGATG 6 cut(s) 262, 453, 632, 823, 927, 952
BstFNI CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
BstH2I RGCGCY 2 cut(s) 282, 375
BstHHI GCGC 6 cut(s) 35, 281, 374, 388, 623, 772
BstKTI GATC 5 cut(s) 133, 184, 209, 509, 837
BstMAI GTCTC 2 cut(s) 575, 730
BstMBI GATC 5 cut(s) 130, 181, 206, 506, 834
BstMWI GCNNNNNNNGC 3 cut(s) 70, 218, 467
BstSFI CTRYAG 3 cut(s) 517, 624, 812
BstSLI GKGCMC 1 cut(s) 734
BstUI CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
BstV1I GCAGC 5 cut(s) 83, 503, 610, 638, 794
BstV2I GAAGAC 1 cut(s) 384
BstXI CCANNNNNNTGG 1 cut(s) 253
BsuRI GGCC 7 cut(s) 77, 201, 470, 666, 804, 810, 863
BtgI CCRYGG 2 cut(s) 57, 202
BtsCI GGATG 6 cut(s) 262, 453, 632, 823, 927, 952
BtsI GCAGTG 3 cut(s) 526, 792, 828
BtsIMutI CAGTG 4 cut(s) 526, 792, 828, 939
BveI ACCTGC 1 cut(s) 515
Cac8I GCNNGC 3 cut(s) 75, 247, 325
CfoI GCGC 6 cut(s) 35, 281, 374, 388, 623, 772
Cfr10I RCCGGY 2 cut(s) 638, 695
Cfr13I GGNCC 2 cut(s) 260, 802
CseI GACGC 1 cut(s) 53
Csp6I GTAC 2 cut(s) 699, 796
CspAI ACCGGT 1 cut(s) 638
CviAII CATG 5 cut(s) 58, 253, 334, 547, 955
CviQI GTAC 2 cut(s) 699, 796
DdeI CTNAG 1 cut(s) 942
DinI GGCGCC 2 cut(s) 280, 373
DpnI GATC 5 cut(s) 132, 183, 208, 508, 836
DpnII GATC 5 cut(s) 130, 181, 206, 506, 834
EaeI YGGCCR 2 cut(s) 199, 664
Ecl136II GAGCTC 1 cut(s) 406
Eco130I CCWWGG 3 cut(s) 57, 610, 805
Eco147I AGGCCT 1 cut(s) 810
Eco24I GRGCYC 2 cut(s) 408, 558
Eco31I GGTCTC 2 cut(s) 575, 730
Eco47I GGWCC 1 cut(s) 260
Eco53kI GAGCTC 1 cut(s) 406
Eco88I CYCGRG 1 cut(s) 578
EcoICRI GAGCTC 1 cut(s) 406
EcoO109I RGGNCCY 1 cut(s) 802
EcoRI GAATTC 1 cut(s) 495
EcoT14I CCWWGG 3 cut(s) 57, 610, 805
EcoT38I GRGCYC 2 cut(s) 408, 558
EgeI GGCGCC 2 cut(s) 280, 373
EheI GGCGCC 2 cut(s) 280, 373
ErhI CCWWGG 3 cut(s) 57, 610, 805
FaeI CATG 5 cut(s) 61, 256, 337, 550, 958
FaiI YATR 9 cut(s) 59, 254, 335, 548, 755, 872, 903, 917, 956
FalI AAGNNNNNCTT 2 cut(s) 831, 863
FaqI GGGAC 2 cut(s) 241, 775
FatI CATG 5 cut(s) 57, 252, 333, 546, 954
FauI CCCGC 4 cut(s) 28, 101, 332, 752
FbaI TGATCA 1 cut(s) 834
FblI GTMKAC 1 cut(s) 42
Fnu4HI GCNGC 8 cut(s) 31, 97, 138, 277, 517, 624, 627, 783
FokI GGATG 6 cut(s) 269, 460, 619, 830, 934, 959
FriOI GRGCYC 2 cut(s) 408, 558
Fsp4HI GCNGC 8 cut(s) 31, 97, 138, 277, 517, 624, 627, 783
FspBI CTAG 3 cut(s) 134, 806, 882
GlaI GCGC 6 cut(s) 34, 280, 373, 387, 622, 771
GluI GCNGC 8 cut(s) 31, 97, 138, 277, 517, 624, 627, 783
HaeII RGCGCY 2 cut(s) 282, 375
HaeIII GGCC 7 cut(s) 77, 201, 470, 666, 804, 810, 863
HapII CCGG 4 cut(s) 369, 639, 696, 864
HgaI GACGC 1 cut(s) 53
HhaI GCGC 6 cut(s) 35, 281, 374, 388, 623, 772
Hin1I GRCGYC 2 cut(s) 279, 372
Hin1II CATG 5 cut(s) 61, 256, 337, 550, 958
Hin6I GCGC 6 cut(s) 33, 279, 372, 386, 621, 770
HinP1I GCGC 6 cut(s) 33, 279, 372, 386, 621, 770
HincII GTYRAC 1 cut(s) 43
HindII GTYRAC 1 cut(s) 43
HinfI GANTC 6 cut(s) 49, 307, 535, 669, 677, 867
HpaII CCGG 4 cut(s) 369, 639, 696, 864
HphI GGTGA 5 cut(s) 82, 336, 686, 749, 902
Hpy166II GTNNAC 5 cut(s) 25, 43, 412, 571, 732
Hpy188I TCNGA 2 cut(s) 130, 511
Hpy188III TCNNGA 2 cut(s) 68, 288
Hpy8I GTNNAC 5 cut(s) 25, 43, 412, 571, 732
HpyAV CCTTC 1 cut(s) 373
HpyCH4III ACNGT 3 cut(s) 232, 301, 586
HpyCH4V TGCA 7 cut(s) 20, 519, 524, 544, 626, 732, 821
HpyF10VI GCNNNNNNNGC 3 cut(s) 70, 218, 467
HpyF3I CTNAG 1 cut(s) 942
Hsp92I GRCGYC 2 cut(s) 279, 372
Hsp92II CATG 5 cut(s) 61, 256, 337, 550, 958
HspAI GCGC 6 cut(s) 33, 279, 372, 386, 621, 770
KasI GGCGCC 2 cut(s) 278, 371
KpnI GGTACC 1 cut(s) 702
Ksp22I TGATCA 1 cut(s) 834
Kzo9I GATC 5 cut(s) 130, 181, 206, 506, 834
LmnI GCTCC 3 cut(s) 61, 131, 561
Lsp1109I GCAGC 5 cut(s) 83, 503, 610, 638, 794
LweI GCATC 5 cut(s) 120, 258, 263, 438, 808
MaeI CTAG 3 cut(s) 134, 806, 882
MaeIII GTNAC 2 cut(s) 674, 710
MalI GATC 5 cut(s) 132, 183, 208, 508, 836
MbiI CCGCTC 1 cut(s) 126
MboI GATC 5 cut(s) 130, 181, 206, 506, 834
MboII GAAGA 2 cut(s) 181, 389
MfeI CAATTG 1 cut(s) 15
MhlI GDGCHC 3 cut(s) 408, 558, 734
MluCI AATT 5 cut(s) 15, 170, 455, 495, 910
MluI ACGCGT 1 cut(s) 705
Mly113I GGCGCC 2 cut(s) 279, 372
MlyI GAGTC 3 cut(s) 544, 671, 861
MseI TTAA 3 cut(s) 293, 303, 909
MslI CAYNNNNRTG 1 cut(s) 875
MspA1I CMGCKG 1 cut(s) 96
MspI CCGG 4 cut(s) 369, 639, 696, 864
MunI CAATTG 1 cut(s) 15
MvnI CGCG 7 cut(s) 35, 386, 388, 707, 759, 770, 772
MwoI GCNNNNNNNGC 3 cut(s) 70, 218, 467
NarI GGCGCC 2 cut(s) 279, 372
NcoI CCATGG 1 cut(s) 57
NdeII GATC 5 cut(s) 130, 181, 206, 506, 834
NlaIII CATG 5 cut(s) 61, 256, 337, 550, 958
NlaIV GGNNCC 6 cut(s) 262, 280, 373, 557, 694, 700
NmeAIII GCCGAG 1 cut(s) 87
NmuCI GTSAC 2 cut(s) 674, 710
PaqCI CACCTGC 1 cut(s) 515
PceI AGGCCT 1 cut(s) 810
PcsI WCGNNNNNNNCGW 2 cut(s) 159, 327
PfeI GAWTC 3 cut(s) 49, 307, 669
PflMI CCANNNNNTGG 1 cut(s) 689
PinAI ACCGGT 1 cut(s) 638
PkrI GCNGC 8 cut(s) 32, 98, 139, 278, 518, 625, 628, 784
PleI GAGTC 3 cut(s) 543, 671, 861
PluTI GGCGCC 2 cut(s) 282, 375
PpsI GAGTC 3 cut(s) 543, 671, 861
Psp124BI GAGCTC 1 cut(s) 408
PspN4I GGNNCC 6 cut(s) 262, 280, 373, 557, 694, 700
PspPI GGNCC 2 cut(s) 260, 802
PstI CTGCAG 2 cut(s) 521, 628
RsaI GTAC 2 cut(s) 700, 797
RsaNI GTAC 2 cut(s) 699, 796
RseI CAYNNNNRTG 1 cut(s) 875
SacI GAGCTC 1 cut(s) 408
SalI GTCGAC 1 cut(s) 41
SaqAI TTAA 3 cut(s) 293, 303, 909
SatI GCNGC 8 cut(s) 31, 97, 138, 277, 517, 624, 627, 783
Sau3AI GATC 5 cut(s) 130, 181, 206, 506, 834
Sau96I GGNCC 2 cut(s) 260, 802
SchI GAGTC 3 cut(s) 544, 671, 861
SduI GDGCHC 3 cut(s) 408, 558, 734
SfaNI GCATC 5 cut(s) 120, 258, 263, 438, 808
SfcI CTRYAG 3 cut(s) 517, 624, 812
SfoI GGCGCC 2 cut(s) 280, 373
SgrAI CRCCGGYG 1 cut(s) 638
SinI GGWCC 1 cut(s) 260
SmiMI CAYNNNNRTG 1 cut(s) 875
Sse9I AATT 5 cut(s) 15, 170, 455, 495, 910
SseBI AGGCCT 1 cut(s) 810
SsiI CCGC 8 cut(s) 30, 35, 94, 124, 138, 276, 325, 759
SspDI GGCGCC 2 cut(s) 278, 371
SspMI CTAG 3 cut(s) 134, 806, 882
SstI GAGCTC 1 cut(s) 408
StuI AGGCCT 1 cut(s) 810
StyI CCWWGG 3 cut(s) 57, 610, 805
TaaI ACNGT 3 cut(s) 232, 301, 586
TaqI TCGA 2 cut(s) 42, 153
TasI AATT 5 cut(s) 15, 170, 455, 495, 910
TatI WGTACW 1 cut(s) 795
TauI GCSGC 3 cut(s) 33, 140, 279
TfiI GAWTC 3 cut(s) 49, 307, 669
Tru1I TTAA 3 cut(s) 293, 303, 909
Tru9I TTAA 3 cut(s) 293, 303, 909
TscAI CASTG 4 cut(s) 526, 792, 828, 946
TseFI GTSAC 2 cut(s) 674, 710
TseI GCWGC 5 cut(s) 96, 516, 623, 626, 782
Tsp45I GTSAC 2 cut(s) 674, 710
TspDTI ATGAA 4 cut(s) 415, 563, 665, 943
TspGWI ACGGA 2 cut(s) 489, 528
TspRI CASTG 4 cut(s) 526, 792, 828, 946
Van91I CCANNNNNTGG 1 cut(s) 689
VneI GTGCAC 1 cut(s) 730
VpaK11BI GGWCC 1 cut(s) 260
XapI RAATTY 1 cut(s) 495
XmaJI CCTAGG 1 cut(s) 805
XmiI GTMKAC 1 cut(s) 42
XspI CTAG 3 cut(s) 134, 806, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.